Rroxscaffold_1G00062840

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012036
Physical Location & Seq
Forward (+)
84870948 .. 84874997
4050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00062840.1

Sequence Viewer

Length: 1593 bp
ATGAAGTCACTCTTGATCATCATCAACACTCTCCTCCCATTCACCCCCTACCTCACCACCTTCCTCCTCCTCCTCCTCTTCCTCGAACAACTCTCCTACCTCAAAAAGAAACGCACCGTTCCCGGTCCCTCCCTCGTCCTCCCATTTCTCGGCAACGCAGTCTCCCTAGTCCGCAACCCGACCCGCTTCTGGGACCTCCAATCCTCCCTCGCCACGTCATCCGGCCTCTCCGCCAACTACATCGTCGGCAACTTCATCCTCTTCATTCGTGACACCTCTCTCTCCCACAAAATCTTCGCCAATGTACGACCCGACGCCTTCACCCTCGTCGGACACCCCTTCGGAAAGAAACTCTTCGGCGAGCACAACCTCATTTACATGACCGGGCAGGAGCACAAAGACCTACGACGTAGTATCGCCCCCAACTTCACTCCCAAAGCCCTCGCCACCTACACCGCGCTCCAGCAGATTATTATACTTGAACACCTCAAAAACTGGGTTAGCCTCACGTCTCGCACCAAGGACCCGATCGCGCTCCGGTTCTTGGTCCGAGACATGAATCTGGAGACTTCCCAGACGGTTTTCGTTGGGCCGTACTTGGCCCCCGACGCCCGCGAGAGGTTCAAGTCGGATTATAACTTCTTCAACGTTGGACTCATGAAGCTGCCCGTTGACCTGCCCGGGACCGCGTTCAGAAACGCCAGGCTGGCTGTGGCGCGCCTCGTCGAAACGCTAGCCGGCTGCGCCAAACAAAGCAAGGCGAAAATGGAAGGAGAGCAACAAGAGCCGACGTGTCTCATCGATTTCTGGATGCAGGAGACGGTAAAGGAGCTCAGCCCCGGCGCGGCTGACATCAGCGACGTCGAGGTCGGCGCTCACCTGTTCGACTTCCTCTTCGCTGCTCAGGACGCCTCCACGTCGTCGCTGCTGTGGGCGGTGGCGCTACTCGACTCGCACCCGGAGGTTCTGGCCAAGGTCAGGGAAGAGGTCGCCGGAGTTTGGGACCCGGAGTCGGACCAGCTGATCACGGCGGACCAGCTGGCGAGGATGAGGTACACGCACGCGGTGGCGCGTGAGGTGGTCAGGTACCGTGCTCCAGCCACCATGGTCCCTCACATTGCGGCGGTTGACTTTCCGTTGACCGAGGTTTACACAGTCCCGAAGGGTACCATTGTGTTTCCCTCCGCCTACGAGTCTTGCTTTCAGGGGTTCACGGAGCCGGGGCGGTTCGACCCGGACCGGTTCTCAGAGGAGAGGCAGGAGGACCGGGTGTACAGGAGGAACTACCTGGCGTTCGGGGCCGGGGCCCACCAGTGCGTGGGGCAGAGATACGCTCTGAACCACCTGGTGCTCTTCATCGCGATGTTCGCCACGTTGCTGGATTTCAAGAGGCACAGAACGGACGGTTGCGATGACATTTCGTTTGTTCCGACGATCTGCCCCAAAGACGATTGCAAGGTTTTCCTCTCAATGCGGTGCCGGCGATTCCCCGCTCTTTCTCTACAGAAAAGGAAAATCAATGAAGAAGTCAAACAACTCACACTAAAGTCAATGATCACCCATCAACTCCAAACTAAAGTCGATAGTCGATGA

Protein Analysis

530

Amino Acids

59.95

Weight (kDa)

8.85

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 41 - 456 2.5e-48 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28850 AT2G28860 AT2G34490 AT2G34500
fragaria_vesca FvH4_3g05750
malus_domestica MD05G1285000.v1.1
prunus_persica Prupe.4G079400_v2.0.a1
pyrus_communis pycom05g26430
rosa_chinensis RchiOBHm_Chr5g0013841
rosa_laevigata RLG00000032049
rosa_multiflora Rmu_sc0005005.1_g000004 Rmu_ssc0000256.1_g000017
rosa_roxburghii Rroxscaffold_1G00062840
rosa_rugosa Rorug05G0012800
rosa_samantha Rh5AG107200 Rh5BG103700 Rh5CG115400 Rh5DG102000
rosa_wichuraiana Rw5G009350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 636
AasI GACNNNNNNGTC 1 cut(s) 866
AatII GACGTC 1 cut(s) 864
Acc36I ACCTGC 1 cut(s) 684
Acc65I GGTACC 2 cut(s) 1086, 1166
AccB1I GGYRCC 3 cut(s) 1086, 1166, 1476
AccB7I CCANNNNNTGG 1 cut(s) 1318
AccBSI CCGCTC 1 cut(s) 1493
AccII CGCG 9 cut(s) 458, 533, 615, 689, 718, 845, 1064, 1072, 1361
AclI AACGTT 1 cut(s) 648
AcoI YGGCCR 1 cut(s) 969
AcyI GRCGYC 4 cut(s) 315, 609, 861, 909
AdeI CACNNNGTG 2 cut(s) 1066, 1348
AfaI GTAC 6 cut(s) 306, 596, 1055, 1088, 1168, 1274
AflIII ACRYGT 1 cut(s) 791
AgeI ACCGGT 1 cut(s) 1239
AgsI TTSAA 4 cut(s) 482, 625, 646, 1387
AhdI GACNNNNNGTC 1 cut(s) 1009
AjiI CACGTC 4 cut(s) 216, 510, 792, 918
AjnI CCWGG 3 cut(s) 701, 1287, 1344
AluBI AGCT 4 cut(s) 664, 832, 1021, 1039
AluI AGCT 4 cut(s) 664, 832, 1021, 1039
Alw21I GWGCWC 5 cut(s) 366, 396, 834, 1096, 1353
Alw26I GTCTC 6 cut(s) 166, 516, 546, 560, 800, 812
Ama87I CYCGRG 1 cut(s) 680
AoxI GGCC 6 cut(s) 223, 590, 600, 969, 1299, 1305
ApaI GGGCCC 1 cut(s) 1309
ApeKI GCWGC 4 cut(s) 664, 741, 899, 925
ArsI GACNNNNNNTTYG 4 cut(s) 878, 910, 1406, 1438
AscI GGCGCGCC 1 cut(s) 716
AsiGI ACCGGT 1 cut(s) 1239
Asp700I GAANNNNTTC 1 cut(s) 353
Asp718I GGTACC 2 cut(s) 1086, 1166
AspLEI GCGC 9 cut(s) 460, 535, 718, 720, 746, 845, 875, 943, 1072
AsuHPI GGTGA 5 cut(s) 34, 46, 313, 869, 1549
AsuNHI GCTAGC 1 cut(s) 733
AvaI CYCGRG 1 cut(s) 680
BaeGI GKGCMC 1 cut(s) 1309
BaeI ACNNNNGTAYC 2 cut(s) 1158, 1191
BalI TGGCCA 1 cut(s) 971
BanI GGYRCC 3 cut(s) 1086, 1166, 1476
BanII GRGCYC 2 cut(s) 834, 1309
Bbv12I GWGCWC 5 cut(s) 366, 396, 834, 1096, 1353
BbvI GCAGC 4 cut(s) 651, 728, 886, 912
BccI CCATC 1 cut(s) 1569
BceAI ACGGC 2 cut(s) 577, 1044
BciT130I CCWGG 3 cut(s) 703, 1289, 1346
BclI TGATCA 3 cut(s) 15, 1023, 1554
BcoDI GTCTC 6 cut(s) 166, 516, 546, 560, 800, 812
BfaI CTAG 2 cut(s) 167, 734
BfmI CTRYAG 1 cut(s) 1502
BfoI RGCGCY 2 cut(s) 876, 944
BfuAI ACCTGC 1 cut(s) 684
BglI GCCNNNNNGGC 1 cut(s) 707
BisI GCNGC 6 cut(s) 665, 742, 846, 900, 926, 1122
BlpI GCTNAGC 1 cut(s) 833
BlsI GCNGC 6 cut(s) 666, 743, 847, 901, 927, 1123
BmeRI GACNNNNNGTC 1 cut(s) 1009
BmeT110I CYCGRG 1 cut(s) 680
BmgBI CACGTC 4 cut(s) 216, 510, 792, 918
BmrI ACTGGG 1 cut(s) 505
BmsI GCATC 1 cut(s) 801
BmtI GCTAGC 1 cut(s) 737
BmuI ACTGGG 1 cut(s) 505
BplI GAGNNNNNCTC 2 cut(s) 1318, 1350
BpmI CTGGAG 3 cut(s) 446, 584, 1080
Bpu10I CCTNAGC 1 cut(s) 903
Bpu1102I GCTNAGC 1 cut(s) 833
Bsa29I ATCGAT 1 cut(s) 801
BsaBI GATNNNNATC 1 cut(s) 20
BsaHI GRCGYC 4 cut(s) 315, 609, 861, 909
BsaJI CCNNGG 8 cut(s) 519, 680, 838, 972, 1104, 1143, 1220, 1302
BsaWI WCCGGW 2 cut(s) 537, 1239
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bse118I RCCGGY 3 cut(s) 737, 1239, 1479
Bse1I ACTGG 2 cut(s) 500, 1312
Bse3DI GCAATG 1 cut(s) 1116
Bse8I GATNNNNATC 1 cut(s) 20
BseBI CCWGG 3 cut(s) 703, 1289, 1346
BseCI ATCGAT 1 cut(s) 801
BseDI CCNNGG 8 cut(s) 519, 680, 838, 972, 1104, 1143, 1220, 1302
BseGI GGATG 4 cut(s) 218, 255, 816, 1053
BseJI GATNNNNATC 1 cut(s) 20
BseMI GCAATG 1 cut(s) 1116
BseMII CTCAG 3 cut(s) 847, 917, 1260
BseNI ACTGG 2 cut(s) 500, 1312
BsePI GCGCGC 1 cut(s) 716
BseRI GAGGAG 6 cut(s) 23, 56, 59, 62, 65, 1265
BseSI GKGCMC 1 cut(s) 1309
BseXI GCAGC 4 cut(s) 651, 728, 886, 912
Bsh1236I CGCG 9 cut(s) 458, 533, 615, 689, 718, 845, 1064, 1072, 1361
Bsh1285I CGRYCG 1 cut(s) 531
BshFI GGCC 6 cut(s) 225, 592, 602, 971, 1301, 1307
BshNI GGYRCC 3 cut(s) 1086, 1166, 1476
BshTI ACCGGT 1 cut(s) 1239
BshVI ATCGAT 1 cut(s) 801
BsiEI CGRYCG 1 cut(s) 531
BsiHKAI GWGCWC 5 cut(s) 366, 396, 834, 1096, 1353
BsiHKCI CYCGRG 1 cut(s) 680
BslFI GGGAC 6 cut(s) 111, 206, 697, 1016, 1094, 1142
BsmAI GTCTC 6 cut(s) 166, 516, 546, 560, 800, 812
BsmBI CGTCTC 2 cut(s) 516, 812
BsmFI GGGAC 6 cut(s) 111, 206, 697, 1016, 1094, 1142
BsnI GGCC 6 cut(s) 225, 592, 602, 971, 1301, 1307
BsoBI CYCGRG 1 cut(s) 680
Bsp120I GGGCCC 1 cut(s) 1305
Bsp1286I GDGCHC 6 cut(s) 366, 396, 834, 1096, 1309, 1353
Bsp1407I TGTACA 1 cut(s) 1272
Bsp143I GATC 5 cut(s) 15, 528, 1023, 1434, 1554
Bsp1720I GCTNAGC 1 cut(s) 833
Bsp19I CCATGG 1 cut(s) 1104
Bsp68I TCGCGA 1 cut(s) 1361
BspANI GGCC 6 cut(s) 225, 592, 602, 971, 1301, 1307
BspCNI CTCAG 3 cut(s) 846, 916, 1259
BspDI ATCGAT 1 cut(s) 801
BspFNI CGCG 9 cut(s) 458, 533, 615, 689, 718, 845, 1064, 1072, 1361
BspHI TCATGA 1 cut(s) 657
BspMI ACCTGC 1 cut(s) 684
BspOI GCTAGC 1 cut(s) 737
BspQI GCTCTTC 1 cut(s) 1358
BspT107I GGYRCC 3 cut(s) 1086, 1166, 1476
BsrBI CCGCTC 1 cut(s) 1493
BsrDI GCAATG 1 cut(s) 1116
BsrFI RCCGGY 3 cut(s) 737, 1239, 1479
BsrGI TGTACA 1 cut(s) 1272
BsrI ACTGG 2 cut(s) 500, 1312
BssAI RCCGGY 3 cut(s) 737, 1239, 1479
BssECI CCNNGG 8 cut(s) 519, 680, 838, 972, 1104, 1143, 1220, 1302
BssHII GCGCGC 1 cut(s) 716
BssMI GATC 5 cut(s) 15, 528, 1023, 1434, 1554
BssNI GRCGYC 4 cut(s) 315, 609, 861, 909
BssT1I CCWWGG 3 cut(s) 519, 972, 1104
Bst2UI CCWGG 3 cut(s) 703, 1289, 1346
Bst4CI ACNGT 6 cut(s) 118, 580, 823, 1091, 1156, 1406
Bst6I CTCTTC 6 cut(s) 83, 266, 359, 899, 978, 1358
BstACI GRCGYC 4 cut(s) 315, 609, 861, 909
BstAUI TGTACA 1 cut(s) 1272
BstC8I GCNNGC 9 cut(s) 362, 613, 708, 718, 735, 739, 1041, 1062, 1481
BstDEI CTNAG 3 cut(s) 833, 903, 1246
BstDSI CCRYGG 1 cut(s) 1104
BstF5I GGATG 4 cut(s) 218, 255, 816, 1053
BstFNI CGCG 9 cut(s) 458, 533, 615, 689, 718, 845, 1064, 1072, 1361
BstH2I RGCGCY 2 cut(s) 876, 944
BstHHI GCGC 9 cut(s) 460, 535, 718, 720, 746, 845, 875, 943, 1072
BstKTI GATC 5 cut(s) 18, 531, 1026, 1437, 1557
BstMAI GTCTC 6 cut(s) 166, 516, 546, 560, 800, 812
BstMBI GATC 5 cut(s) 15, 528, 1023, 1434, 1554
BstMCI CGRYCG 1 cut(s) 531
BstMWI GCNNNNNNNGC 8 cut(s) 608, 707, 743, 784, 908, 1298, 1367, 1480
BstNI CCWGG 3 cut(s) 703, 1289, 1346
BstSFI CTRYAG 1 cut(s) 1502
BstSLI GKGCMC 1 cut(s) 1309
BstUI CGCG 9 cut(s) 458, 533, 615, 689, 718, 845, 1064, 1072, 1361
BstV1I GCAGC 4 cut(s) 651, 728, 886, 912
BstXI CCANNNNNNTGG 1 cut(s) 1378
Bsu15I ATCGAT 1 cut(s) 801
BsuRI GGCC 6 cut(s) 225, 592, 602, 971, 1301, 1307
BsuTUI ATCGAT 1 cut(s) 801
BtgI CCRYGG 1 cut(s) 1104
BtgZI GCGATG 3 cut(s) 1342, 1376, 1425
BtrI CACGTC 4 cut(s) 216, 510, 792, 918
BtsCI GGATG 4 cut(s) 218, 255, 816, 1053
BtsIMutI CAGTG 1 cut(s) 1319
BtuMI TCGCGA 1 cut(s) 1361
BveI ACCTGC 1 cut(s) 684
Cac8I GCNNGC 9 cut(s) 362, 613, 708, 718, 735, 739, 1041, 1062, 1481
CciI TCATGA 1 cut(s) 657
CfoI GCGC 9 cut(s) 460, 535, 718, 720, 746, 845, 875, 943, 1072
Cfr10I RCCGGY 3 cut(s) 737, 1239, 1479
Cfr9I CCCGGG 1 cut(s) 680
ClaI ATCGAT 1 cut(s) 801
CpoI CGGWCCG 1 cut(s) 1237
CseI GACGC 3 cut(s) 323, 617, 917
CsiI ACCWGGT 1 cut(s) 1344
Csp6I GTAC 6 cut(s) 305, 595, 1054, 1087, 1167, 1273
CspAI ACCGGT 1 cut(s) 1239
CspI CGGWCCG 1 cut(s) 1237
CviAII CATG 4 cut(s) 379, 556, 658, 1105
CviQI GTAC 6 cut(s) 305, 595, 1054, 1087, 1167, 1273
DdeI CTNAG 3 cut(s) 833, 903, 1246
DpnI GATC 5 cut(s) 17, 530, 1025, 1436, 1556
DpnII GATC 5 cut(s) 15, 528, 1023, 1434, 1554
DraIII CACNNNGTG 2 cut(s) 1066, 1348
DrdI GACNNNNNNGTC 1 cut(s) 866
DriI GACNNNNNGTC 1 cut(s) 1009
DseDI GACNNNNNNGTC 1 cut(s) 866
EaeI YGGCCR 1 cut(s) 969
Eam1104I CTCTTC 6 cut(s) 83, 266, 359, 899, 978, 1358
Eam1105I GACNNNNNGTC 1 cut(s) 1009
EarI CTCTTC 6 cut(s) 83, 266, 359, 899, 978, 1358
EciI GGCGGA 3 cut(s) 220, 1046, 1174
Ecl136II GAGCTC 1 cut(s) 832
Eco130I CCWWGG 3 cut(s) 519, 972, 1104
Eco24I GRGCYC 2 cut(s) 834, 1309
Eco53kI GAGCTC 1 cut(s) 832
Eco88I CYCGRG 1 cut(s) 680
EcoICRI GAGCTC 1 cut(s) 832
EcoO109I RGGNCCY 4 cut(s) 193, 523, 1003, 1305
EcoRII CCWGG 3 cut(s) 701, 1287, 1344
EcoT14I CCWWGG 3 cut(s) 519, 972, 1104
EcoT38I GRGCYC 2 cut(s) 834, 1309
ErhI CCWWGG 3 cut(s) 519, 972, 1104
Esp3I CGTCTC 2 cut(s) 516, 812
FaeI CATG 4 cut(s) 382, 559, 661, 1108
FaiI YATR 6 cut(s) 380, 476, 557, 636, 659, 1106
FalI AAGNNNNNCTT 3 cut(s) 28, 338, 370
FaqI GGGAC 6 cut(s) 111, 206, 697, 1016, 1094, 1142
FatI CATG 4 cut(s) 378, 555, 657, 1104
FauI CCCGC 3 cut(s) 191, 620, 1498
FbaI TGATCA 3 cut(s) 15, 1023, 1554
Fnu4HI GCNGC 6 cut(s) 665, 742, 846, 900, 926, 1122
FokI GGATG 4 cut(s) 205, 242, 823, 1060
FriOI GRGCYC 2 cut(s) 834, 1309
Fsp4HI GCNGC 6 cut(s) 665, 742, 846, 900, 926, 1122
FspBI CTAG 2 cut(s) 167, 734
GlaI GCGC 9 cut(s) 459, 534, 717, 719, 745, 844, 874, 942, 1071
GluI GCNGC 6 cut(s) 665, 742, 846, 900, 926, 1122
GsuI CTGGAG 3 cut(s) 446, 584, 1080
HaeII RGCGCY 2 cut(s) 876, 944
HaeIII GGCC 6 cut(s) 225, 592, 602, 971, 1301, 1307
HgaI GACGC 3 cut(s) 323, 617, 917
HhaI GCGC 9 cut(s) 460, 535, 718, 720, 746, 845, 875, 943, 1072
Hin1I GRCGYC 4 cut(s) 315, 609, 861, 909
Hin1II CATG 4 cut(s) 382, 559, 661, 1108
Hin6I GCGC 9 cut(s) 458, 533, 716, 718, 744, 843, 873, 941, 1070
HinP1I GCGC 9 cut(s) 458, 533, 716, 718, 744, 843, 873, 941, 1070
HincII GTYRAC 3 cut(s) 673, 1129, 1140
HindII GTYRAC 3 cut(s) 673, 1129, 1140
HinfI GANTC 6 cut(s) 559, 654, 950, 1010, 1193, 1485
HphI GGTGA 5 cut(s) 34, 46, 313, 869, 1549
Hpy166II GTNNAC 7 cut(s) 673, 1056, 1129, 1140, 1150, 1212, 1273
Hpy188I TCNGA 9 cut(s) 332, 344, 551, 631, 695, 1015, 1249, 1338, 1431
Hpy188III TCNNGA 9 cut(s) 13, 269, 563, 658, 808, 905, 1159, 1360, 1387
Hpy8I GTNNAC 7 cut(s) 673, 1056, 1129, 1140, 1150, 1212, 1273
HpyAV CCTTC 5 cut(s) 70, 328, 349, 764, 1156
HpyCH4III ACNGT 6 cut(s) 118, 580, 823, 1091, 1156, 1406
HpyCH4IV ACGT 8 cut(s) 215, 409, 509, 648, 791, 861, 917, 1373
HpyCH4V TGCA 2 cut(s) 814, 1455
HpyF10VI GCNNNNNNNGC 8 cut(s) 608, 707, 743, 784, 908, 1298, 1367, 1480
HpyF3I CTNAG 3 cut(s) 833, 903, 1246
HpySE526I ACGT 8 cut(s) 215, 409, 509, 648, 791, 861, 917, 1373
Hsp92I GRCGYC 4 cut(s) 315, 609, 861, 909
Hsp92II CATG 4 cut(s) 382, 559, 661, 1108
HspAI GCGC 9 cut(s) 458, 533, 716, 718, 744, 843, 873, 941, 1070
KflI GGGWCCC 1 cut(s) 1003
KpnI GGTACC 2 cut(s) 1090, 1170
KroI GCCGGC 2 cut(s) 737, 1479
KroNI GCCGGC 2 cut(s) 739, 1481
Ksp22I TGATCA 3 cut(s) 15, 1023, 1554
Kzo9I GATC 5 cut(s) 15, 528, 1023, 1434, 1554
LguI GCTCTTC 1 cut(s) 1358
LmnI GCTCC 6 cut(s) 391, 465, 540, 829, 1099, 1216
Lsp1109I GCAGC 4 cut(s) 651, 728, 886, 912
LweI GCATC 1 cut(s) 801
MabI ACCWGGT 1 cut(s) 1344
MaeI CTAG 2 cut(s) 167, 734
MaeII ACGT 8 cut(s) 215, 409, 509, 648, 791, 861, 917, 1373
MaeIII GTNAC 2 cut(s) 6, 269
MalI GATC 5 cut(s) 17, 530, 1025, 1436, 1556
MbiI CCGCTC 1 cut(s) 1493
MboI GATC 5 cut(s) 15, 528, 1023, 1434, 1554
MboII GAAGA 9 cut(s) 70, 253, 286, 346, 634, 886, 995, 1345, 1535
MhlI GDGCHC 6 cut(s) 366, 396, 834, 1096, 1309, 1353
MlsI TGGCCA 1 cut(s) 971
MluNI TGGCCA 1 cut(s) 971
MlyI GAGTC 4 cut(s) 648, 944, 1019, 1202
MmeI TCCRAC 5 cut(s) 310, 609, 631, 993, 1454
Mox20I TGGCCA 1 cut(s) 971
MroNI GCCGGC 2 cut(s) 737, 1479
MroXI GAANNNNTTC 1 cut(s) 353
MscI TGGCCA 1 cut(s) 971
MslI CAYNNNNRTG 2 cut(s) 377, 1361
Msp20I TGGCCA 1 cut(s) 971
MspA1I CMGCKG 2 cut(s) 1021, 1039
MvaI CCWGG 3 cut(s) 703, 1289, 1346
MvnI CGCG 9 cut(s) 458, 533, 615, 689, 718, 845, 1064, 1072, 1361
MwoI GCNNNNNNNGC 8 cut(s) 608, 707, 743, 784, 908, 1298, 1367, 1480
NaeI GCCGGC 2 cut(s) 739, 1481
NcoI CCATGG 1 cut(s) 1104
NdeII GATC 5 cut(s) 15, 528, 1023, 1434, 1554
NgoMIV GCCGGC 2 cut(s) 737, 1479
NheI GCTAGC 1 cut(s) 733
NlaIII CATG 4 cut(s) 382, 559, 661, 1108
NmeAIII GCCGAG 1 cut(s) 129
NmuCI GTSAC 2 cut(s) 6, 269
NruI TCGCGA 1 cut(s) 1361
PagI TCATGA 1 cut(s) 657
PalAI GGCGCGCC 1 cut(s) 716
PauI GCGCGC 1 cut(s) 716
PciSI GCTCTTC 1 cut(s) 1358
PcsI WCGNNNNNNNCGW 1 cut(s) 1427
PdiI GCCGGC 2 cut(s) 739, 1481
PdmI GAANNNNTTC 1 cut(s) 353
PfeI GAWTC 2 cut(s) 559, 1485
PflMI CCANNNNNTGG 1 cut(s) 1318
PinAI ACCGGT 1 cut(s) 1239
PkrI GCNGC 6 cut(s) 666, 743, 847, 901, 927, 1123
Ple19I CGATCG 1 cut(s) 531
PleI GAGTC 4 cut(s) 648, 944, 1018, 1201
PpsI GAGTC 4 cut(s) 648, 944, 1018, 1201
PpuMI RGGWCCY 3 cut(s) 193, 523, 1003
PsiI TTATAA 1 cut(s) 636
Psp124BI GAGCTC 1 cut(s) 834
Psp1406I AACGTT 1 cut(s) 648
Psp5II RGGWCCY 3 cut(s) 193, 523, 1003
Psp6I CCWGG 3 cut(s) 701, 1287, 1344
PspGI CCWGG 3 cut(s) 701, 1287, 1344
PspOMI GGGCCC 1 cut(s) 1305
PspPPI RGGWCCY 3 cut(s) 193, 523, 1003
PteI GCGCGC 1 cut(s) 716
PvuI CGATCG 1 cut(s) 531
PvuII CAGCTG 2 cut(s) 1021, 1039
RruI TCGCGA 1 cut(s) 1361
RsaI GTAC 6 cut(s) 306, 596, 1055, 1088, 1168, 1274
RsaNI GTAC 6 cut(s) 305, 595, 1054, 1087, 1167, 1273
RseI CAYNNNNRTG 2 cut(s) 377, 1361
Rsr2I CGGWCCG 1 cut(s) 1237
RsrII CGGWCCG 1 cut(s) 1237
SacI GAGCTC 1 cut(s) 834
SapI GCTCTTC 1 cut(s) 1358
SatI GCNGC 6 cut(s) 665, 742, 846, 900, 926, 1122
Sau3AI GATC 5 cut(s) 15, 528, 1023, 1434, 1554
SchI GAGTC 4 cut(s) 648, 944, 1019, 1202
SduI GDGCHC 6 cut(s) 366, 396, 834, 1096, 1309, 1353
SexAI ACCWGGT 1 cut(s) 1344
SfaNI GCATC 1 cut(s) 801
SfcI CTRYAG 1 cut(s) 1502
SgsI GGCGCGCC 1 cut(s) 716
SmaI CCCGGG 1 cut(s) 682
SmiMI CAYNNNNRTG 2 cut(s) 377, 1361
SspMI CTAG 2 cut(s) 167, 734
SstI GAGCTC 1 cut(s) 834
StyI CCWWGG 3 cut(s) 519, 972, 1104
TaaI ACNGT 6 cut(s) 118, 580, 823, 1091, 1156, 1406
TaiI ACGT 8 cut(s) 218, 412, 512, 651, 794, 864, 920, 1376
TaqI TCGA 9 cut(s) 84, 726, 801, 864, 885, 948, 1230, 1581, 1588
TaqII GACCGA 1 cut(s) 1157
TatI WGTACW 1 cut(s) 1272
TauI GCSGC 2 cut(s) 848, 1124
TfiI GAWTC 2 cut(s) 559, 1485
TscAI CASTG 1 cut(s) 1319
TseFI GTSAC 2 cut(s) 6, 269
TseI GCWGC 4 cut(s) 664, 741, 899, 925
Tsp45I GTSAC 2 cut(s) 6, 269
TspDTI ATGAA 7 cut(s) 17, 244, 253, 572, 674, 1345, 1536
TspGWI ACGGA 3 cut(s) 1125, 1229, 1415
TspMI CCCGGG 1 cut(s) 680
TspRI CASTG 1 cut(s) 1319
Van91I CCANNNNNTGG 1 cut(s) 1318
XcmI CCANNNNNNNNNTGG 1 cut(s) 709
XmaI CCCGGG 1 cut(s) 680
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 2 cut(s) 167, 734
ZraI GACGTC 1 cut(s) 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.