Rh5AG107200

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0012036
Physical Location & Seq
Reverse (-)
9811349 .. 9812852
1504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG107200.1

Sequence Viewer

Length: 747 bp
ATGCTTGCCTGCTGCGCCAAACAAAGCAAGGCGAAAATGGAAGGAGAGCAACAAGAGCCGACGTGTCTCATCGATTTCTGGATGCAGGAGACAGTAAAGGAGCTCAGCGCCGGCGCGGCTGACATCAGTGACGTCGAGGTAGGCGCTCACCTGTTCGACTTCCTCTTCGCTGCTCAGGACGCCTCCACGTCGTCTCTGCTGTGGGCGGTGGCACTACTCGACTCGCACCAGGAGGTTCTGGCCAAGGTCCGGGAAGAGGTCGCTGGAGTTTGGGACCCCGAGTCGGACGAGCTGATCACAGCTGAACAGCTGGCGCGGATGAGGTACACGCACTCGGTGGCACGTGAGGTGGTCAGGTACCGTGCTCCAGCCACCATGGTCCCTCACATTGCAGCGGTTGACTTTCCGTTGACCGAGGCGTACACAGTCCCGAAGGGTACCATTGTGTTTCCCTCCGCCTACGAGTCTTGCTTTCAGGGGTTCCCGGAGCCGGAGAGGTTCGACCCGGACCGGTTCTCGGAGGAGAGGCAGGAGGACCGGCTGTACAGGAGGAACTACCTGGCGTTTGGGGCCGGGGCCCACCAGTGCCTGGGGCAGAGATACGCTCTGAACCACCTGGTGCTCTTCATCGCGATGTTCGCCACGTTGCTGGATTTCAAGAGGCACAGGACGGACGGCTGCGATGACATTTCGTTTGTTCCGACGATCTGCCCCAAAGACGATTGCAAGTTTCATACATATCGTTAA

Protein Analysis

248

Amino Acids

28.1

Weight (kDa)

5.04

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 5 - 214 1.7e-40 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28850 AT2G28860 AT2G34490 AT2G34500
fragaria_vesca FvH4_3g05750
malus_domestica MD05G1285000.v1.1
prunus_persica Prupe.4G079400_v2.0.a1
pyrus_communis pycom05g26430
rosa_chinensis RchiOBHm_Chr5g0013841
rosa_laevigata RLG00000032049
rosa_multiflora Rmu_sc0005005.1_g000004 Rmu_ssc0000256.1_g000017
rosa_roxburghii Rroxscaffold_1G00062840
rosa_rugosa Rorug05G0012800
rosa_samantha Rh5AG107200 Rh5BG103700 Rh5CG115400 Rh5DG102000
rosa_wichuraiana Rw5G009350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 135
Acc65I GGTACC 2 cut(s) 357, 437
AccB1I GGYRCC 2 cut(s) 357, 437
AccB7I CCANNNNNTGG 1 cut(s) 589
AccII CGCG 3 cut(s) 116, 316, 632
AciI CCGC 5 cut(s) 116, 206, 316, 395, 456
AcoI YGGCCR 1 cut(s) 240
AcvI CACGTG 1 cut(s) 344
AcyI GRCGYC 2 cut(s) 132, 180
AdeI CACNNNGTG 2 cut(s) 337, 619
AfaI GTAC 5 cut(s) 326, 359, 422, 439, 545
AfiI CCNNNNNNNGG 6 cut(s) 249, 256, 283, 490, 517, 589
AflIII ACRYGT 1 cut(s) 62
AgeI ACCGGT 1 cut(s) 510
AgsI TTSAA 1 cut(s) 658
AhdI GACNNNNNGTC 1 cut(s) 280
AjiI CACGTC 2 cut(s) 63, 189
AjnI CCWGG 4 cut(s) 228, 558, 588, 615
AluBI AGCT 4 cut(s) 103, 292, 302, 310
AluI AGCT 4 cut(s) 103, 292, 302, 310
Alw21I GWGCWC 3 cut(s) 105, 367, 624
Alw26I GTCTC 3 cut(s) 71, 83, 198
AlwNI CAGNNNCTG 1 cut(s) 589
Ama87I CYCGRG 1 cut(s) 278
AoxI GGCC 3 cut(s) 240, 570, 576
ApaI GGGCCC 1 cut(s) 580
ApeKI GCWGC 4 cut(s) 12, 170, 392, 678
ArsI GACNNNNNNTTYG 4 cut(s) 149, 181, 677, 709
AsiGI ACCGGT 1 cut(s) 510
Asp718I GGTACC 2 cut(s) 357, 437
AspLEI GCGC 5 cut(s) 17, 110, 116, 146, 316
AspS9I GGNCC 8 cut(s) 247, 274, 379, 508, 535, 570, 576, 577
AsuC2I CCSGG 4 cut(s) 251, 485, 506, 574
AsuHPI GGTGA 1 cut(s) 140
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 5 cut(s) 247, 274, 379, 508, 535
BaeGI GKGCMC 1 cut(s) 580
BaeI ACNNNNGTAYC 2 cut(s) 429, 462
BalI TGGCCA 1 cut(s) 242
BanI GGYRCC 2 cut(s) 357, 437
BanII GRGCYC 2 cut(s) 105, 580
BbrPI CACGTG 1 cut(s) 344
Bbv12I GWGCWC 3 cut(s) 105, 367, 624
BbvI GCAGC 3 cut(s) 157, 404, 665
BceAI ACGGC 1 cut(s) 691
BciT130I CCWGG 4 cut(s) 230, 560, 590, 617
BclI TGATCA 1 cut(s) 294
BcnI CCSGG 4 cut(s) 251, 485, 506, 574
BcoDI GTCTC 3 cut(s) 71, 83, 198
BfoI RGCGCY 2 cut(s) 111, 147
BglI GCCNNNNNGGC 1 cut(s) 116
BisI GCNGC 5 cut(s) 13, 117, 171, 393, 679
BlpI GCTNAGC 1 cut(s) 104
BlsI GCNGC 5 cut(s) 14, 118, 172, 394, 680
Bme1390I CCNGG 8 cut(s) 230, 251, 485, 506, 560, 574, 590, 617
Bme18I GGWCC 5 cut(s) 247, 274, 379, 508, 535
BmeRI GACNNNNNGTC 1 cut(s) 280
BmeT110I CYCGRG 1 cut(s) 278
BmgBI CACGTC 2 cut(s) 63, 189
BmgT120I GGNCC 8 cut(s) 247, 274, 379, 508, 535, 570, 576, 577
BmrFI CCNGG 8 cut(s) 230, 251, 485, 506, 560, 574, 590, 617
BmsI GCATC 1 cut(s) 72
BplI GAGNNNNNCTC 2 cut(s) 589, 621
BpmI CTGGAG 2 cut(s) 285, 351
Bpu10I CCTNAGC 1 cut(s) 174
Bpu1102I GCTNAGC 1 cut(s) 104
BpuMI CCSGG 4 cut(s) 251, 485, 506, 574
Bsa29I ATCGAT 1 cut(s) 72
BsaAI YACGTR 1 cut(s) 344
BsaHI GRCGYC 2 cut(s) 132, 180
BsaJI CCNNGG 5 cut(s) 243, 375, 414, 573, 589
BsaWI WCCGGW 1 cut(s) 510
Bsc4I CCNNNNNNNGG 6 cut(s) 249, 256, 283, 490, 517, 589
Bse118I RCCGGY 3 cut(s) 110, 510, 537
Bse1I ACTGG 1 cut(s) 583
Bse3DI GCAATG 1 cut(s) 387
BseBI CCWGG 4 cut(s) 230, 560, 590, 617
BseCI ATCGAT 1 cut(s) 72
BseDI CCNNGG 5 cut(s) 243, 375, 414, 573, 589
BseGI GGATG 2 cut(s) 87, 324
BseLI CCNNNNNNNGG 6 cut(s) 249, 256, 283, 490, 517, 589
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 2 cut(s) 118, 188
BseNI ACTGG 1 cut(s) 583
BseRI GAGGAG 1 cut(s) 536
BseSI GKGCMC 1 cut(s) 580
BseXI GCAGC 3 cut(s) 157, 404, 665
Bsh1236I CGCG 3 cut(s) 116, 316, 632
BshFI GGCC 3 cut(s) 242, 572, 578
BshNI GGYRCC 2 cut(s) 357, 437
BshTI ACCGGT 1 cut(s) 510
BshVI ATCGAT 1 cut(s) 72
BsiHKAI GWGCWC 3 cut(s) 105, 367, 624
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 8 cut(s) 111, 250, 485, 491, 506, 511, 538, 573
BslFI GGGAC 3 cut(s) 287, 365, 413
BslI CCNNNNNNNGG 6 cut(s) 249, 256, 283, 490, 517, 589
BsmAI GTCTC 3 cut(s) 71, 83, 198
BsmBI CGTCTC 1 cut(s) 198
BsmFI GGGAC 3 cut(s) 287, 365, 413
BsnI GGCC 3 cut(s) 242, 572, 578
BsoBI CYCGRG 1 cut(s) 278
Bsp120I GGGCCC 1 cut(s) 576
Bsp1286I GDGCHC 4 cut(s) 105, 367, 580, 624
Bsp1407I TGTACA 1 cut(s) 543
Bsp143I GATC 2 cut(s) 294, 705
Bsp1720I GCTNAGC 1 cut(s) 104
Bsp19I CCATGG 1 cut(s) 375
Bsp68I TCGCGA 1 cut(s) 632
BspACI CCGC 5 cut(s) 116, 206, 316, 395, 456
BspANI GGCC 3 cut(s) 242, 572, 578
BspCNI CTCAG 2 cut(s) 117, 187
BspDI ATCGAT 1 cut(s) 72
BspFNI CGCG 3 cut(s) 116, 316, 632
BspQI GCTCTTC 1 cut(s) 629
BspT107I GGYRCC 2 cut(s) 357, 437
BsrDI GCAATG 1 cut(s) 387
BsrFI RCCGGY 3 cut(s) 110, 510, 537
BsrGI TGTACA 1 cut(s) 543
BsrI ACTGG 1 cut(s) 583
BssAI RCCGGY 3 cut(s) 110, 510, 537
BssECI CCNNGG 5 cut(s) 243, 375, 414, 573, 589
BssMI GATC 2 cut(s) 294, 705
BssNI GRCGYC 2 cut(s) 132, 180
BssT1I CCWWGG 2 cut(s) 243, 375
Bst2UI CCWGG 4 cut(s) 230, 560, 590, 617
Bst4CI ACNGT 3 cut(s) 94, 362, 427
Bst6I CTCTTC 3 cut(s) 170, 249, 629
BstACI GRCGYC 2 cut(s) 132, 180
BstAUI TGTACA 1 cut(s) 543
BstBAI YACGTR 1 cut(s) 344
BstC8I GCNNGC 4 cut(s) 6, 10, 112, 312
BstDEI CTNAG 2 cut(s) 104, 174
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 2 cut(s) 87, 324
BstFNI CGCG 3 cut(s) 116, 316, 632
BstH2I RGCGCY 2 cut(s) 111, 147
BstHHI GCGC 5 cut(s) 17, 110, 116, 146, 316
BstKTI GATC 2 cut(s) 297, 708
BstMAI GTCTC 3 cut(s) 71, 83, 198
BstMBI GATC 2 cut(s) 294, 705
BstMWI GCNNNNNNNGC 6 cut(s) 14, 55, 116, 179, 569, 638
BstNI CCWGG 4 cut(s) 230, 560, 590, 617
BstSCI CCNGG 8 cut(s) 228, 249, 483, 504, 558, 572, 588, 615
BstSLI GKGCMC 1 cut(s) 580
BstUI CGCG 3 cut(s) 116, 316, 632
BstV1I GCAGC 3 cut(s) 157, 404, 665
BstXI CCANNNNNNTGG 1 cut(s) 649
Bsu15I ATCGAT 1 cut(s) 72
BsuRI GGCC 3 cut(s) 242, 572, 578
BsuTUI ATCGAT 1 cut(s) 72
BtgI CCRYGG 1 cut(s) 375
BtgZI GCGATG 3 cut(s) 613, 647, 696
BtrI CACGTC 2 cut(s) 63, 189
BtsCI GGATG 2 cut(s) 87, 324
BtsIMutI CAGTG 2 cut(s) 133, 590
BtuMI TCGCGA 1 cut(s) 632
Cac8I GCNNGC 4 cut(s) 6, 10, 112, 312
CaiI CAGNNNCTG 1 cut(s) 589
CfoI GCGC 5 cut(s) 17, 110, 116, 146, 316
Cfr10I RCCGGY 3 cut(s) 110, 510, 537
Cfr13I GGNCC 8 cut(s) 247, 274, 379, 508, 535, 570, 576, 577
ClaI ATCGAT 1 cut(s) 72
CpoI CGGWCCG 1 cut(s) 508
CseI GACGC 1 cut(s) 188
CsiI ACCWGGT 1 cut(s) 615
Csp6I GTAC 5 cut(s) 325, 358, 421, 438, 544
CspAI ACCGGT 1 cut(s) 510
CspI CGGWCCG 1 cut(s) 508
CviAII CATG 1 cut(s) 376
CviQI GTAC 5 cut(s) 325, 358, 421, 438, 544
DdeI CTNAG 2 cut(s) 104, 174
DpnI GATC 2 cut(s) 296, 707
DpnII GATC 2 cut(s) 294, 705
DraIII CACNNNGTG 2 cut(s) 337, 619
DriI GACNNNNNGTC 1 cut(s) 280
EaeI YGGCCR 1 cut(s) 240
Eam1104I CTCTTC 3 cut(s) 170, 249, 629
Eam1105I GACNNNNNGTC 1 cut(s) 280
EarI CTCTTC 3 cut(s) 170, 249, 629
EciI GGCGGA 1 cut(s) 445
Ecl136II GAGCTC 1 cut(s) 103
Eco130I CCWWGG 2 cut(s) 243, 375
Eco24I GRGCYC 2 cut(s) 105, 580
Eco47I GGWCC 5 cut(s) 247, 274, 379, 508, 535
Eco53kI GAGCTC 1 cut(s) 103
Eco72I CACGTG 1 cut(s) 344
Eco88I CYCGRG 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 103
EcoO109I RGGNCCY 2 cut(s) 274, 576
EcoRII CCWGG 4 cut(s) 228, 558, 588, 615
EcoT14I CCWWGG 2 cut(s) 243, 375
EcoT38I GRGCYC 2 cut(s) 105, 580
ErhI CCWWGG 2 cut(s) 243, 375
Esp3I CGTCTC 1 cut(s) 198
FaeI CATG 1 cut(s) 379
FaiI YATR 3 cut(s) 377, 735, 739
FaqI GGGAC 3 cut(s) 287, 365, 413
FatI CATG 1 cut(s) 375
FbaI TGATCA 1 cut(s) 294
Fnu4HI GCNGC 5 cut(s) 13, 117, 171, 393, 679
FokI GGATG 2 cut(s) 94, 331
FriOI GRGCYC 2 cut(s) 105, 580
Fsp4HI GCNGC 5 cut(s) 13, 117, 171, 393, 679
GlaI GCGC 5 cut(s) 16, 109, 115, 145, 315
GluI GCNGC 5 cut(s) 13, 117, 171, 393, 679
GsuI CTGGAG 2 cut(s) 285, 351
HaeII RGCGCY 2 cut(s) 111, 147
HaeIII GGCC 3 cut(s) 242, 572, 578
HapII CCGG 8 cut(s) 111, 250, 485, 491, 506, 511, 538, 573
HgaI GACGC 1 cut(s) 188
HhaI GCGC 5 cut(s) 17, 110, 116, 146, 316
Hin1I GRCGYC 2 cut(s) 132, 180
Hin1II CATG 1 cut(s) 379
Hin6I GCGC 5 cut(s) 15, 108, 114, 144, 314
HinP1I GCGC 5 cut(s) 15, 108, 114, 144, 314
HincII GTYRAC 2 cut(s) 400, 411
HindII GTYRAC 2 cut(s) 400, 411
HinfI GANTC 3 cut(s) 221, 281, 464
HpaII CCGG 8 cut(s) 111, 250, 485, 491, 506, 511, 538, 573
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 4 cut(s) 327, 400, 411, 423
Hpy188I TCNGA 4 cut(s) 286, 520, 609, 702
Hpy188III TCNNGA 5 cut(s) 79, 176, 430, 631, 658
Hpy8I GTNNAC 4 cut(s) 327, 400, 411, 423
Hpy99I CGWCG 4 cut(s) 64, 137, 193, 706
HpyAV CCTTC 2 cut(s) 35, 427
HpyCH4III ACNGT 3 cut(s) 94, 362, 427
HpyCH4IV ACGT 5 cut(s) 62, 132, 188, 343, 644
HpyCH4V TGCA 3 cut(s) 85, 392, 726
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 55, 116, 179, 569, 638
HpyF3I CTNAG 2 cut(s) 104, 174
HpySE526I ACGT 5 cut(s) 62, 132, 188, 343, 644
Hsp92I GRCGYC 2 cut(s) 132, 180
Hsp92II CATG 1 cut(s) 379
HspAI GCGC 5 cut(s) 15, 108, 114, 144, 314
KflI GGGWCCC 1 cut(s) 274
KpnI GGTACC 2 cut(s) 361, 441
KroI GCCGGC 1 cut(s) 110
KroNI GCCGGC 1 cut(s) 112
Ksp22I TGATCA 1 cut(s) 294
Kzo9I GATC 2 cut(s) 294, 705
LguI GCTCTTC 1 cut(s) 629
LmnI GCTCC 3 cut(s) 100, 370, 487
Lsp1109I GCAGC 3 cut(s) 157, 404, 665
LweI GCATC 1 cut(s) 72
MabI ACCWGGT 1 cut(s) 615
MaeII ACGT 5 cut(s) 62, 132, 188, 343, 644
MaeIII GTNAC 1 cut(s) 128
MalI GATC 2 cut(s) 296, 707
MboI GATC 2 cut(s) 294, 705
MboII GAAGA 3 cut(s) 157, 266, 616
MhlI GDGCHC 4 cut(s) 105, 367, 580, 624
MlsI TGGCCA 1 cut(s) 242
MluNI TGGCCA 1 cut(s) 242
MlyI GAGTC 3 cut(s) 215, 290, 473
MmeI TCCRAC 2 cut(s) 264, 725
Mox20I TGGCCA 1 cut(s) 242
MreI CGCCGGCG 1 cut(s) 110
MroNI GCCGGC 1 cut(s) 110
MscI TGGCCA 1 cut(s) 242
MseI TTAA 1 cut(s) 745
MslI CAYNNNNRTG 1 cut(s) 632
Msp20I TGGCCA 1 cut(s) 242
MspA1I CMGCKG 3 cut(s) 302, 310, 395
MspI CCGG 8 cut(s) 111, 250, 485, 491, 506, 511, 538, 573
MspR9I CCNGG 8 cut(s) 230, 251, 485, 506, 560, 574, 590, 617
MvaI CCWGG 4 cut(s) 230, 560, 590, 617
MvnI CGCG 3 cut(s) 116, 316, 632
MwoI GCNNNNNNNGC 6 cut(s) 14, 55, 116, 179, 569, 638
NaeI GCCGGC 1 cut(s) 112
NciI CCSGG 4 cut(s) 251, 485, 506, 574
NcoI CCATGG 1 cut(s) 375
NdeII GATC 2 cut(s) 294, 705
NgoMIV GCCGGC 1 cut(s) 110
NlaIII CATG 1 cut(s) 379
NmuCI GTSAC 1 cut(s) 128
NruI TCGCGA 1 cut(s) 632
PciSI GCTCTTC 1 cut(s) 629
PcsI WCGNNNNNNNCGW 1 cut(s) 698
PdiI GCCGGC 1 cut(s) 112
PflMI CCANNNNNTGG 1 cut(s) 589
PfoI TCCNGGA 2 cut(s) 249, 483
PinAI ACCGGT 1 cut(s) 510
PkrI GCNGC 5 cut(s) 14, 118, 172, 394, 680
PleI GAGTC 3 cut(s) 215, 289, 472
PmaCI CACGTG 1 cut(s) 344
PmlI CACGTG 1 cut(s) 344
PpsI GAGTC 3 cut(s) 215, 289, 472
Ppu21I YACGTR 1 cut(s) 344
PpuMI RGGWCCY 1 cut(s) 274
Psp124BI GAGCTC 1 cut(s) 105
Psp5II RGGWCCY 1 cut(s) 274
Psp6I CCWGG 4 cut(s) 228, 558, 588, 615
PspCI CACGTG 1 cut(s) 344
PspGI CCWGG 4 cut(s) 228, 558, 588, 615
PspOMI GGGCCC 1 cut(s) 576
PspPI GGNCC 8 cut(s) 247, 274, 379, 508, 535, 570, 576, 577
PspPPI RGGWCCY 1 cut(s) 274
PstNI CAGNNNCTG 1 cut(s) 589
PvuII CAGCTG 2 cut(s) 302, 310
RruI TCGCGA 1 cut(s) 632
RsaI GTAC 5 cut(s) 326, 359, 422, 439, 545
RsaNI GTAC 5 cut(s) 325, 358, 421, 438, 544
RseI CAYNNNNRTG 1 cut(s) 632
Rsr2I CGGWCCG 1 cut(s) 508
RsrII CGGWCCG 1 cut(s) 508
SacI GAGCTC 1 cut(s) 105
SapI GCTCTTC 1 cut(s) 629
SaqAI TTAA 1 cut(s) 745
SatI GCNGC 5 cut(s) 13, 117, 171, 393, 679
Sau3AI GATC 2 cut(s) 294, 705
Sau96I GGNCC 8 cut(s) 247, 274, 379, 508, 535, 570, 576, 577
SchI GAGTC 3 cut(s) 215, 290, 473
ScrFI CCNGG 8 cut(s) 230, 251, 485, 506, 560, 574, 590, 617
SduI GDGCHC 4 cut(s) 105, 367, 580, 624
SexAI ACCWGGT 1 cut(s) 615
SfaNI GCATC 1 cut(s) 72
SgrAI CRCCGGYG 1 cut(s) 110
SinI GGWCC 5 cut(s) 247, 274, 379, 508, 535
SmiMI CAYNNNNRTG 1 cut(s) 632
SsiI CCGC 5 cut(s) 116, 206, 316, 395, 456
SstI GAGCTC 1 cut(s) 105
StyD4I CCNGG 8 cut(s) 228, 249, 483, 504, 558, 572, 588, 615
StyI CCWWGG 2 cut(s) 243, 375
TaaI ACNGT 3 cut(s) 94, 362, 427
TaiI ACGT 5 cut(s) 65, 135, 191, 346, 647
TaqI TCGA 5 cut(s) 72, 135, 156, 219, 501
TaqII GACCGA 1 cut(s) 428
TatI WGTACW 1 cut(s) 543
TauI GCSGC 1 cut(s) 119
Tru1I TTAA 1 cut(s) 745
Tru9I TTAA 1 cut(s) 745
TscAI CASTG 2 cut(s) 133, 590
TseFI GTSAC 1 cut(s) 128
TseI GCWGC 4 cut(s) 12, 170, 392, 678
Tsp45I GTSAC 1 cut(s) 128
TspDTI ATGAA 2 cut(s) 616, 722
TspGWI ACGGA 2 cut(s) 396, 686
TspRI CASTG 2 cut(s) 133, 590
Van91I CCANNNNNTGG 1 cut(s) 589
VpaK11BI GGWCC 5 cut(s) 247, 274, 379, 508, 535
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.