Rh5BG103700

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012036
Physical Location & Seq
Reverse (-)
9477973 .. 9480593
2621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG103700.1

Sequence Viewer

Length: 1509 bp
ATGAAGTCACTCTTGATCATCATCAACACTCTCCTCCCACTCACCCCCTACCTCACCACCTTCCTCCTCCTCCTCCTCTTCCTTGAACAACTCTCCTACCTCAAAAAGAAACGCACCGTTCCCGGTCCCTCCCTCGTCCTCCCATTTCTCGGCAACGCAGTCTCCCTAGTACGCAACCCGACCCGCTTCTGGGACCTCCAATCCTCCCTCGCCACGTCATCCGGCCTCTCCGCCAACTACATCGTCGGCAACTTCATCCTCTTCATCCGCGACACCTCTCTCTCCCACAAAGTCTTCGCCAATGTACGACCCGACGCCTTCACCCTCGTCGGCCACCCCTTCGGAAAGAAACTCTTCGGCGAGCACAACCTCATTTACATGACCGGCCAGGAGCACAAAGACCTACGACGTCGTATCGCCCCCAACTTCACTCCCAAAGCCCTCGCCACCTACACCGCGCTCCAGCAGATTATTATCCTTGAACACCTCAAAAACTGGGTTAGCCTCACGTCTCAGACCAAGGACCCGATCGCGCTCCGGTTCTTGGTCCGAGACATGAATCTGGAGACTTCCCAGACGGTTTTCGTTGGGCCATACTTGGCCCCCGACGCCCGCGAGAGGTTCAAGTCGGATTATAACTTCTTCAACGTTGGACTCATGAAGCTGCCCGTTGACCTGCCCGGGACCGCGTTCAGAAACGCCAGGCTGGCTGTGGTGCGCCTCGTCGAAACGCTCGCCGGCTGCGCCAAACAAAGCAGGGCGAAAATGGAAGCAGAACAACAAGAGCCGACGTGTTTAATCGATTTCTGGATGCAGGAGACGGTAAAGGAGCTCAGCGCCGGCGTGGCTGACATCAGCGACGTCGAGGTCGGCGCTCACCTGTTCGACTTTCTATTTGCTGCCCAGGACGCCTCAACTTCGTCGCTGCTGTGGGCGGTGGTGCTGCTCGACTCGCACCCGGAGGTTCTGGCCAAGGTCCGGGAAGAGGTCGCTGGAGTTTGGGACCCTGAGTCGGACGAGCTGATAACGGCGGGGCAGCTGGTGCGGATGAGGTACACGCATGCAGTGGCGCGTGAGGTGGTCAGGTACCGTGCTCCGGCCACCATGGTCCCTCACATTGCGGCGGTTGACTTTCCGTTGACCGATACGTACACAGTCCCCAAGGGTACCATTGTGTTTCCCTCCGCCTACGAGTCTTGCTTTCAGGGGTTCACGGAACCGGAGCGGTTCGACCCGGACCGGTTCTCGGAGGAGAGACAGGAGGACCGGGTGTACAGGAGGAACTACCTGGCGTTCGGGGCCGGGGCCCACCAGTGCGTGGGGCAGAGATACGCTCTGAACCACCTGGTGCTCTTCATCGCGGTGTTCGCCACGTTGCTGGATTTCAAGAGGCACAGAACGGACGGCTGCGATGACATCTCGTACGTTCCGACGATCTGCCCCAAAGACGATTGCAAGGTTTTCCTCTCGATGCGGTGCCGCCGATTCCCCGCTCTATATCTACAGTGA

Protein Analysis

502

Amino Acids

56.8

Weight (kDa)

8.04

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 41 - 456 1.8e-49 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012036)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28850 AT2G28860 AT2G34490 AT2G34500
fragaria_vesca FvH4_3g05750
malus_domestica MD05G1285000.v1.1
prunus_persica Prupe.4G079400_v2.0.a1
pyrus_communis pycom05g26430
rosa_chinensis RchiOBHm_Chr5g0013841
rosa_laevigata RLG00000032049
rosa_multiflora Rmu_sc0005005.1_g000004 Rmu_ssc0000256.1_g000017
rosa_roxburghii Rroxscaffold_1G00062840
rosa_rugosa Rorug05G0012800
rosa_samantha Rh5AG107200 Rh5BG103700 Rh5CG115400 Rh5DG102000
rosa_wichuraiana Rw5G009350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 636
AasI GACNNNNNNGTC 1 cut(s) 866
AatII GACGTC 2 cut(s) 412, 864
Acc36I ACCTGC 1 cut(s) 684
Acc65I GGTACC 2 cut(s) 1086, 1166
AccB1I GGYRCC 3 cut(s) 1086, 1166, 1476
AccB7I CCANNNNNTGG 1 cut(s) 1318
AccBSI CCGCTC 2 cut(s) 1225, 1493
AccII CGCG 7 cut(s) 270, 458, 533, 615, 689, 1072, 1361
AclI AACGTT 1 cut(s) 648
AcoI YGGCCR 4 cut(s) 331, 385, 969, 1098
AcyI GRCGYC 5 cut(s) 315, 409, 609, 861, 909
AdeI CACNNNGTG 1 cut(s) 1348
AfaI GTAC 8 cut(s) 171, 306, 1055, 1088, 1151, 1168, 1274, 1424
AflIII ACRYGT 1 cut(s) 791
AgeI ACCGGT 1 cut(s) 1239
AgsI TTSAA 5 cut(s) 86, 482, 625, 646, 1387
AhdI GACNNNNNGTC 1 cut(s) 1009
AjiI CACGTC 3 cut(s) 216, 510, 792
AjnI CCWGG 5 cut(s) 387, 701, 903, 1287, 1344
AluBI AGCT 4 cut(s) 664, 832, 1021, 1039
AluI AGCT 4 cut(s) 664, 832, 1021, 1039
Alw21I GWGCWC 5 cut(s) 366, 396, 834, 1096, 1353
Alw26I GTCTC 6 cut(s) 166, 516, 546, 560, 812, 1249
Ama87I CYCGRG 1 cut(s) 680
AoxI GGCC 9 cut(s) 223, 331, 385, 590, 600, 969, 1098, 1299, 1305
ApaI GGGCCC 1 cut(s) 1309
ApeKI GCWGC 7 cut(s) 664, 741, 899, 925, 943, 1036, 1407
ArsI GACNNNNNNTTYG 2 cut(s) 878, 910
AsiGI ACCGGT 1 cut(s) 1239
Asp700I GAANNNNTTC 1 cut(s) 353
Asp718I GGTACC 2 cut(s) 1086, 1166
AspLEI GCGC 7 cut(s) 460, 535, 720, 746, 839, 875, 1072
AsuC2I CCSGG 8 cut(s) 123, 681, 682, 959, 980, 1235, 1268, 1303
AsuHPI GGTGA 4 cut(s) 34, 46, 313, 869
AvaI CYCGRG 1 cut(s) 680
BaeGI GKGCMC 1 cut(s) 1309
BaeI ACNNNNGTAYC 2 cut(s) 1158, 1191
BalI TGGCCA 1 cut(s) 971
BanI GGYRCC 3 cut(s) 1086, 1166, 1476
BanII GRGCYC 2 cut(s) 834, 1309
BbsI GAAGAC 1 cut(s) 286
Bbv12I GWGCWC 5 cut(s) 366, 396, 834, 1096, 1353
BbvI GCAGC 7 cut(s) 651, 728, 886, 912, 930, 1048, 1394
BceAI ACGGC 2 cut(s) 1044, 1420
BciT130I CCWGG 5 cut(s) 389, 703, 905, 1289, 1346
BclI TGATCA 1 cut(s) 15
BcnI CCSGG 8 cut(s) 123, 681, 682, 959, 980, 1235, 1268, 1303
BcoDI GTCTC 6 cut(s) 166, 516, 546, 560, 812, 1249
BfaI CTAG 1 cut(s) 167
BfmI CTRYAG 1 cut(s) 1502
BfoI RGCGCY 2 cut(s) 840, 876
BfuAI ACCTGC 1 cut(s) 684
BglI GCCNNNNNGGC 2 cut(s) 707, 845
BisI GCNGC 9 cut(s) 665, 742, 900, 926, 944, 1037, 1122, 1408, 1480
BlpI GCTNAGC 1 cut(s) 833
BlsI GCNGC 9 cut(s) 666, 743, 901, 927, 945, 1038, 1123, 1409, 1481
BmeRI GACNNNNNGTC 1 cut(s) 1009
BmeT110I CYCGRG 1 cut(s) 680
BmgBI CACGTC 3 cut(s) 216, 510, 792
BmrI ACTGGG 1 cut(s) 505
BmsI GCATC 2 cut(s) 801, 1461
BmuI ACTGGG 1 cut(s) 505
BpiI GAAGAC 1 cut(s) 286
BplI GAGNNNNNCTC 2 cut(s) 1318, 1350
BpmI CTGGAG 3 cut(s) 446, 584, 1014
Bpu1102I GCTNAGC 1 cut(s) 833
BpuMI CCSGG 8 cut(s) 123, 681, 682, 959, 980, 1235, 1268, 1303
Bsa29I ATCGAT 1 cut(s) 801
BsaAI YACGTR 1 cut(s) 1149
BsaBI GATNNNNATC 2 cut(s) 20, 473
BsaHI GRCGYC 5 cut(s) 315, 409, 609, 861, 909
BsaJI CCNNGG 7 cut(s) 519, 680, 903, 972, 1104, 1161, 1302
BsaWI WCCGGW 3 cut(s) 537, 1219, 1239
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bse118I RCCGGY 4 cut(s) 383, 737, 839, 1239
Bse1I ACTGG 2 cut(s) 500, 1312
Bse3DI GCAATG 1 cut(s) 1116
Bse8I GATNNNNATC 2 cut(s) 20, 473
BseBI CCWGG 5 cut(s) 389, 703, 905, 1289, 1346
BseCI ATCGAT 1 cut(s) 801
BseDI CCNNGG 7 cut(s) 519, 680, 903, 972, 1104, 1161, 1302
BseGI GGATG 5 cut(s) 218, 255, 264, 816, 1053
BseJI GATNNNNATC 2 cut(s) 20, 473
BseMI GCAATG 1 cut(s) 1116
BseMII CTCAG 3 cut(s) 527, 847, 999
BseNI ACTGG 2 cut(s) 500, 1312
BseRI GAGGAG 6 cut(s) 23, 56, 59, 62, 65, 1265
BseSI GKGCMC 1 cut(s) 1309
BseXI GCAGC 7 cut(s) 651, 728, 886, 912, 930, 1048, 1394
Bsh1236I CGCG 7 cut(s) 270, 458, 533, 615, 689, 1072, 1361
Bsh1285I CGRYCG 1 cut(s) 531
BshFI GGCC 9 cut(s) 225, 333, 387, 592, 602, 971, 1100, 1301, 1307
BshNI GGYRCC 3 cut(s) 1086, 1166, 1476
BshTI ACCGGT 1 cut(s) 1239
BshVI ATCGAT 1 cut(s) 801
BsiEI CGRYCG 1 cut(s) 531
BsiHKAI GWGCWC 5 cut(s) 366, 396, 834, 1096, 1353
BsiHKCI CYCGRG 1 cut(s) 680
BsiWI CGTACG 1 cut(s) 1422
BslFI GGGAC 6 cut(s) 111, 206, 697, 1016, 1094, 1142
BsmAI GTCTC 6 cut(s) 166, 516, 546, 560, 812, 1249
BsmBI CGTCTC 2 cut(s) 516, 812
BsmFI GGGAC 6 cut(s) 111, 206, 697, 1016, 1094, 1142
BsnI GGCC 9 cut(s) 225, 333, 387, 592, 602, 971, 1100, 1301, 1307
BsoBI CYCGRG 1 cut(s) 680
Bsp120I GGGCCC 1 cut(s) 1305
Bsp1286I GDGCHC 6 cut(s) 366, 396, 834, 1096, 1309, 1353
Bsp1407I TGTACA 1 cut(s) 1272
Bsp143I GATC 3 cut(s) 15, 528, 1434
Bsp1720I GCTNAGC 1 cut(s) 833
Bsp19I CCATGG 1 cut(s) 1104
BspANI GGCC 9 cut(s) 225, 333, 387, 592, 602, 971, 1100, 1301, 1307
BspCNI CTCAG 3 cut(s) 526, 846, 1000
BspDI ATCGAT 1 cut(s) 801
BspFNI CGCG 7 cut(s) 270, 458, 533, 615, 689, 1072, 1361
BspHI TCATGA 1 cut(s) 657
BspMI ACCTGC 1 cut(s) 684
BspQI GCTCTTC 1 cut(s) 1358
BspT107I GGYRCC 3 cut(s) 1086, 1166, 1476
BsrBI CCGCTC 2 cut(s) 1225, 1493
BsrDI GCAATG 1 cut(s) 1116
BsrFI RCCGGY 4 cut(s) 383, 737, 839, 1239
BsrGI TGTACA 1 cut(s) 1272
BsrI ACTGG 2 cut(s) 500, 1312
BssAI RCCGGY 4 cut(s) 383, 737, 839, 1239
BssECI CCNNGG 7 cut(s) 519, 680, 903, 972, 1104, 1161, 1302
BssMI GATC 3 cut(s) 15, 528, 1434
BssNI GRCGYC 5 cut(s) 315, 409, 609, 861, 909
BssT1I CCWWGG 4 cut(s) 519, 972, 1104, 1161
Bst2UI CCWGG 5 cut(s) 389, 703, 905, 1289, 1346
Bst4CI ACNGT 6 cut(s) 118, 580, 823, 1091, 1156, 1506
Bst6I CTCTTC 5 cut(s) 83, 266, 359, 978, 1358
BstACI GRCGYC 5 cut(s) 315, 409, 609, 861, 909
BstAPI GCANNNNNTGC 1 cut(s) 1042
BstAUI TGTACA 1 cut(s) 1272
BstBAI YACGTR 1 cut(s) 1149
BstC8I GCNNGC 7 cut(s) 362, 613, 708, 735, 739, 841, 1062
BstDEI CTNAG 3 cut(s) 513, 833, 1008
BstDSI CCRYGG 1 cut(s) 1104
BstF5I GGATG 5 cut(s) 218, 255, 264, 816, 1053
BstFNI CGCG 7 cut(s) 270, 458, 533, 615, 689, 1072, 1361
BstH2I RGCGCY 2 cut(s) 840, 876
BstHHI GCGC 7 cut(s) 460, 535, 720, 746, 839, 875, 1072
BstKTI GATC 3 cut(s) 18, 531, 1437
BstMAI GTCTC 6 cut(s) 166, 516, 546, 560, 812, 1249
BstMBI GATC 3 cut(s) 15, 528, 1434
BstMCI CGRYCG 1 cut(s) 531
BstMWI GCNNNNNNNGC 9 cut(s) 608, 707, 743, 845, 908, 952, 1042, 1298, 1367
BstNI CCWGG 5 cut(s) 389, 703, 905, 1289, 1346
BstNSI RCATGY 1 cut(s) 1064
BstSFI CTRYAG 1 cut(s) 1502
BstSLI GKGCMC 1 cut(s) 1309
BstSNI TACGTA 1 cut(s) 1149
BstUI CGCG 7 cut(s) 270, 458, 533, 615, 689, 1072, 1361
BstV1I GCAGC 7 cut(s) 651, 728, 886, 912, 930, 1048, 1394
BstV2I GAAGAC 1 cut(s) 286
BstXI CCANNNNNNTGG 1 cut(s) 1378
Bsu15I ATCGAT 1 cut(s) 801
BsuRI GGCC 9 cut(s) 225, 333, 387, 592, 602, 971, 1100, 1301, 1307
BsuTUI ATCGAT 1 cut(s) 801
BtgI CCRYGG 1 cut(s) 1104
BtgZI GCGATG 2 cut(s) 1342, 1425
BtrI CACGTC 3 cut(s) 216, 510, 792
BtsCI GGATG 5 cut(s) 218, 255, 264, 816, 1053
BtsI GCAGTG 1 cut(s) 1071
BtsIMutI CAGTG 2 cut(s) 1071, 1319
BveI ACCTGC 1 cut(s) 684
Cac8I GCNNGC 7 cut(s) 362, 613, 708, 735, 739, 841, 1062
CciI TCATGA 1 cut(s) 657
CfoI GCGC 7 cut(s) 460, 535, 720, 746, 839, 875, 1072
Cfr10I RCCGGY 4 cut(s) 383, 737, 839, 1239
Cfr9I CCCGGG 1 cut(s) 680
ClaI ATCGAT 1 cut(s) 801
CpoI CGGWCCG 1 cut(s) 1237
CseI GACGC 3 cut(s) 323, 617, 917
CsiI ACCWGGT 1 cut(s) 1344
Csp6I GTAC 8 cut(s) 170, 305, 1054, 1087, 1150, 1167, 1273, 1423
CspAI ACCGGT 1 cut(s) 1239
CspI CGGWCCG 1 cut(s) 1237
CviAII CATG 5 cut(s) 379, 556, 658, 1061, 1105
CviQI GTAC 8 cut(s) 170, 305, 1054, 1087, 1150, 1167, 1273, 1423
DdeI CTNAG 3 cut(s) 513, 833, 1008
DpnI GATC 3 cut(s) 17, 530, 1436
DpnII GATC 3 cut(s) 15, 528, 1434
DraIII CACNNNGTG 1 cut(s) 1348
DrdI GACNNNNNNGTC 1 cut(s) 866
DriI GACNNNNNGTC 1 cut(s) 1009
DseDI GACNNNNNNGTC 1 cut(s) 866
EaeI YGGCCR 4 cut(s) 331, 385, 969, 1098
Eam1104I CTCTTC 5 cut(s) 83, 266, 359, 978, 1358
Eam1105I GACNNNNNGTC 1 cut(s) 1009
EarI CTCTTC 5 cut(s) 83, 266, 359, 978, 1358
EciI GGCGGA 2 cut(s) 220, 1174
Ecl136II GAGCTC 1 cut(s) 832
Eco105I TACGTA 1 cut(s) 1149
Eco130I CCWWGG 4 cut(s) 519, 972, 1104, 1161
Eco24I GRGCYC 2 cut(s) 834, 1309
Eco53kI GAGCTC 1 cut(s) 832
Eco88I CYCGRG 1 cut(s) 680
EcoICRI GAGCTC 1 cut(s) 832
EcoO109I RGGNCCY 4 cut(s) 193, 523, 1003, 1305
EcoRII CCWGG 5 cut(s) 387, 701, 903, 1287, 1344
EcoT14I CCWWGG 4 cut(s) 519, 972, 1104, 1161
EcoT38I GRGCYC 2 cut(s) 834, 1309
ErhI CCWWGG 4 cut(s) 519, 972, 1104, 1161
Esp3I CGTCTC 2 cut(s) 516, 812
FaeI CATG 5 cut(s) 382, 559, 661, 1064, 1108
FaiI YATR 8 cut(s) 380, 557, 595, 636, 659, 1062, 1106, 1498
FalI AAGNNNNNCTT 3 cut(s) 28, 338, 370
FaqI GGGAC 6 cut(s) 111, 206, 697, 1016, 1094, 1142
FatI CATG 5 cut(s) 378, 555, 657, 1060, 1104
FauI CCCGC 4 cut(s) 191, 620, 1024, 1498
FbaI TGATCA 1 cut(s) 15
Fnu4HI GCNGC 9 cut(s) 665, 742, 900, 926, 944, 1037, 1122, 1408, 1480
FokI GGATG 5 cut(s) 205, 242, 251, 823, 1060
FriOI GRGCYC 2 cut(s) 834, 1309
Fsp4HI GCNGC 9 cut(s) 665, 742, 900, 926, 944, 1037, 1122, 1408, 1480
FspBI CTAG 1 cut(s) 167
GlaI GCGC 7 cut(s) 459, 534, 719, 745, 838, 874, 1071
GluI GCNGC 9 cut(s) 665, 742, 900, 926, 944, 1037, 1122, 1408, 1480
GsuI CTGGAG 3 cut(s) 446, 584, 1014
HaeII RGCGCY 2 cut(s) 840, 876
HaeIII GGCC 9 cut(s) 225, 333, 387, 592, 602, 971, 1100, 1301, 1307
HgaI GACGC 3 cut(s) 323, 617, 917
HhaI GCGC 7 cut(s) 460, 535, 720, 746, 839, 875, 1072
Hin1I GRCGYC 5 cut(s) 315, 409, 609, 861, 909
Hin1II CATG 5 cut(s) 382, 559, 661, 1064, 1108
Hin6I GCGC 7 cut(s) 458, 533, 718, 744, 837, 873, 1070
HinP1I GCGC 7 cut(s) 458, 533, 718, 744, 837, 873, 1070
HincII GTYRAC 3 cut(s) 673, 1129, 1140
HindII GTYRAC 3 cut(s) 673, 1129, 1140
HinfI GANTC 6 cut(s) 559, 654, 950, 1010, 1193, 1485
HphI GGTGA 4 cut(s) 34, 46, 313, 869
Hpy166II GTNNAC 7 cut(s) 673, 1056, 1129, 1140, 1152, 1212, 1273
Hpy188I TCNGA 9 cut(s) 344, 516, 551, 631, 695, 1015, 1249, 1338, 1431
Hpy188III TCNNGA 6 cut(s) 13, 563, 658, 808, 1387, 1468
Hpy8I GTNNAC 7 cut(s) 673, 1056, 1129, 1140, 1152, 1212, 1273
HpyAV CCTTC 3 cut(s) 70, 328, 349
HpyCH4III ACNGT 6 cut(s) 118, 580, 823, 1091, 1156, 1506
HpyCH4IV ACGT 9 cut(s) 215, 409, 509, 648, 791, 861, 1148, 1373, 1425
HpyCH4V TGCA 3 cut(s) 814, 1064, 1455
HpyF10VI GCNNNNNNNGC 9 cut(s) 608, 707, 743, 845, 908, 952, 1042, 1298, 1367
HpyF3I CTNAG 3 cut(s) 513, 833, 1008
HpySE526I ACGT 9 cut(s) 215, 409, 509, 648, 791, 861, 1148, 1373, 1425
Hsp92I GRCGYC 5 cut(s) 315, 409, 609, 861, 909
Hsp92II CATG 5 cut(s) 382, 559, 661, 1064, 1108
HspAI GCGC 7 cut(s) 458, 533, 718, 744, 837, 873, 1070
KflI GGGWCCC 1 cut(s) 1003
KpnI GGTACC 2 cut(s) 1090, 1170
KroI GCCGGC 2 cut(s) 737, 839
KroNI GCCGGC 2 cut(s) 739, 841
Ksp22I TGATCA 1 cut(s) 15
Kzo9I GATC 3 cut(s) 15, 528, 1434
LguI GCTCTTC 1 cut(s) 1358
LmnI GCTCC 6 cut(s) 391, 465, 540, 829, 1099, 1222
Lsp1109I GCAGC 7 cut(s) 651, 728, 886, 912, 930, 1048, 1394
LweI GCATC 2 cut(s) 801, 1461
MabI ACCWGGT 1 cut(s) 1344
MaeI CTAG 1 cut(s) 167
MaeII ACGT 9 cut(s) 215, 409, 509, 648, 791, 861, 1148, 1373, 1425
MaeIII GTNAC 1 cut(s) 6
MalI GATC 3 cut(s) 17, 530, 1436
MbiI CCGCTC 2 cut(s) 1225, 1493
MboI GATC 3 cut(s) 15, 528, 1434
MboII GAAGA 7 cut(s) 70, 253, 286, 346, 634, 995, 1345
MhlI GDGCHC 6 cut(s) 366, 396, 834, 1096, 1309, 1353
MlsI TGGCCA 1 cut(s) 971
MluNI TGGCCA 1 cut(s) 971
MlyI GAGTC 4 cut(s) 648, 944, 1019, 1202
MmeI TCCRAC 4 cut(s) 609, 631, 993, 1454
Mox20I TGGCCA 1 cut(s) 971
MreI CGCCGGCG 1 cut(s) 839
MroNI GCCGGC 2 cut(s) 737, 839
MroXI GAANNNNTTC 1 cut(s) 353
MscI TGGCCA 1 cut(s) 971
MseI TTAA 1 cut(s) 797
MslI CAYNNNNRTG 2 cut(s) 377, 1361
Msp20I TGGCCA 1 cut(s) 971
MspA1I CMGCKG 1 cut(s) 1039
MvaI CCWGG 5 cut(s) 389, 703, 905, 1289, 1346
MvnI CGCG 7 cut(s) 270, 458, 533, 615, 689, 1072, 1361
MwoI GCNNNNNNNGC 9 cut(s) 608, 707, 743, 845, 908, 952, 1042, 1298, 1367
NaeI GCCGGC 2 cut(s) 739, 841
NciI CCSGG 8 cut(s) 123, 681, 682, 959, 980, 1235, 1268, 1303
NcoI CCATGG 1 cut(s) 1104
NdeII GATC 3 cut(s) 15, 528, 1434
NgoMIV GCCGGC 2 cut(s) 737, 839
NlaIII CATG 5 cut(s) 382, 559, 661, 1064, 1108
NmeAIII GCCGAG 1 cut(s) 129
NmuCI GTSAC 1 cut(s) 6
NspI RCATGY 1 cut(s) 1064
PaeI GCATGC 1 cut(s) 1064
PagI TCATGA 1 cut(s) 657
PciSI GCTCTTC 1 cut(s) 1358
PcsI WCGNNNNNNNCGW 1 cut(s) 1427
PdiI GCCGGC 2 cut(s) 739, 841
PdmI GAANNNNTTC 1 cut(s) 353
PfeI GAWTC 2 cut(s) 559, 1485
Pfl23II CGTACG 1 cut(s) 1422
PflMI CCANNNNNTGG 1 cut(s) 1318
PfoI TCCNGGA 1 cut(s) 978
PinAI ACCGGT 1 cut(s) 1239
PkrI GCNGC 9 cut(s) 666, 743, 901, 927, 945, 1038, 1123, 1409, 1481
Ple19I CGATCG 1 cut(s) 531
PleI GAGTC 4 cut(s) 648, 944, 1018, 1201
PpsI GAGTC 4 cut(s) 648, 944, 1018, 1201
Ppu21I YACGTR 1 cut(s) 1149
PpuMI RGGWCCY 3 cut(s) 193, 523, 1003
PsiI TTATAA 1 cut(s) 636
Psp124BI GAGCTC 1 cut(s) 834
Psp1406I AACGTT 1 cut(s) 648
Psp5II RGGWCCY 3 cut(s) 193, 523, 1003
Psp6I CCWGG 5 cut(s) 387, 701, 903, 1287, 1344
PspGI CCWGG 5 cut(s) 387, 701, 903, 1287, 1344
PspLI CGTACG 1 cut(s) 1422
PspOMI GGGCCC 1 cut(s) 1305
PspPPI RGGWCCY 3 cut(s) 193, 523, 1003
PvuI CGATCG 1 cut(s) 531
PvuII CAGCTG 1 cut(s) 1039
RsaI GTAC 8 cut(s) 171, 306, 1055, 1088, 1151, 1168, 1274, 1424
RsaNI GTAC 8 cut(s) 170, 305, 1054, 1087, 1150, 1167, 1273, 1423
RseI CAYNNNNRTG 2 cut(s) 377, 1361
Rsr2I CGGWCCG 1 cut(s) 1237
RsrII CGGWCCG 1 cut(s) 1237
SacI GAGCTC 1 cut(s) 834
SapI GCTCTTC 1 cut(s) 1358
SaqAI TTAA 1 cut(s) 797
SatI GCNGC 9 cut(s) 665, 742, 900, 926, 944, 1037, 1122, 1408, 1480
Sau3AI GATC 3 cut(s) 15, 528, 1434
SchI GAGTC 4 cut(s) 648, 944, 1019, 1202
SduI GDGCHC 6 cut(s) 366, 396, 834, 1096, 1309, 1353
SexAI ACCWGGT 1 cut(s) 1344
SfaNI GCATC 2 cut(s) 801, 1461
SfcI CTRYAG 1 cut(s) 1502
SgrAI CRCCGGYG 1 cut(s) 839
SmaI CCCGGG 1 cut(s) 682
SmiMI CAYNNNNRTG 2 cut(s) 377, 1361
SnaBI TACGTA 1 cut(s) 1149
SphI GCATGC 1 cut(s) 1064
SspMI CTAG 1 cut(s) 167
SstI GAGCTC 1 cut(s) 834
StyI CCWWGG 4 cut(s) 519, 972, 1104, 1161
TaaI ACNGT 6 cut(s) 118, 580, 823, 1091, 1156, 1506
TaiI ACGT 9 cut(s) 218, 412, 512, 651, 794, 864, 1151, 1376, 1428
TaqI TCGA 7 cut(s) 726, 801, 864, 885, 948, 1230, 1469
TaqII GACCGA 1 cut(s) 1157
TatI WGTACW 1 cut(s) 1272
TauI GCSGC 2 cut(s) 1124, 1482
TfiI GAWTC 2 cut(s) 559, 1485
Tru1I TTAA 1 cut(s) 797
Tru9I TTAA 1 cut(s) 797
TscAI CASTG 2 cut(s) 1071, 1319
TseFI GTSAC 1 cut(s) 6
TseI GCWGC 7 cut(s) 664, 741, 899, 925, 943, 1036, 1407
Tsp45I GTSAC 1 cut(s) 6
TspDTI ATGAA 6 cut(s) 17, 244, 253, 572, 674, 1345
TspGWI ACGGA 3 cut(s) 1125, 1229, 1415
TspMI CCCGGG 1 cut(s) 680
TspRI CASTG 2 cut(s) 1071, 1319
Van91I CCANNNNNTGG 1 cut(s) 1318
XceI RCATGY 1 cut(s) 1064
XcmI CCANNNNNNNNNTGG 1 cut(s) 709
XmaI CCCGGG 1 cut(s) 680
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 1 cut(s) 167
ZraI GACGTC 2 cut(s) 410, 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.