Rroxscaffold_1G00070220

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0025683
Physical Location & Seq
Forward (+)
91037977 .. 91052737
14761 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00070220.1

Sequence Viewer

Length: 546 bp
ATGAGGTGGAGGCGTGGAGCTGCTGTCATCATATCCAAGAAAGCTCGCGATGTTCCTCGTGCCAAGGCCATGGATTACGTTGGTGGATCGATTCCAACTTCTAGGGATACTCTTCGAGAATATATTGGAAAATATGATCCATCAGAGCTATTGATCTATGATTTTGATACCATCTTAACTGCTACAGACAATTTCAGCATTACAACCAAACTCAGGCAAGGAGGCTTTGGGTATACTACAAGATGGGAAGGAAATAGCTGTAAAAGACTATCTAGTAGCTCAGGACAATGCATTGAAGAGTTCAAGAATGAGATGTTGTTGATGTCCAATCTTCAACGCAAAAATCTTGTTAGGATCACGGGTTGCTGTGTTAAAGAGGATGAGAAGTTGCTGGTTTATAAGTTCATGCCGAACAAAAAAGCTTGGACACGTTTCTATTCGGTAGTTTCACTACCACTTTATGATCTATTCGGTTCGGGACGTAACAACCTCTATGGGGGTTCTCTCGCTGAGAACAATTGTCCCCATATTGATGCATTCATTTGA

Protein Analysis

181

Amino Acids

20.62

Weight (kDa)

9.3

Isoelectric Point (pI)

49.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 88 - 138 8.5e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0025683)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8162910.1_g000001
rosa_roxburghii Rroxscaffold_1G00070220
rosa_samantha Rh4BG020800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 399
AccI GTMKAC 1 cut(s) 233
AccII CGCG 1 cut(s) 48
AclWI GGATC 3 cut(s) 94, 131, 362
AfiI CCNNNNNNNGG 2 cut(s) 213, 496
AflIII ACRYGT 1 cut(s) 428
AgsI TTSAA 3 cut(s) 296, 304, 335
AluBI AGCT 6 cut(s) 20, 44, 148, 258, 279, 422
AluI AGCT 6 cut(s) 20, 44, 148, 258, 279, 422
AlwI GGATC 3 cut(s) 94, 131, 362
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 1 cut(s) 20
BauI CACGAG 1 cut(s) 57
BbvI GCAGC 1 cut(s) 7
BccI CCATC 3 cut(s) 148, 179, 237
BciVI GTATCC 1 cut(s) 100
BfaI CTAG 2 cut(s) 102, 273
BfmI CTRYAG 1 cut(s) 183
BfuI GTATCC 1 cut(s) 100
BisI GCNGC 1 cut(s) 21
BlsI GCNGC 1 cut(s) 22
BmsI GCATC 1 cut(s) 523
Bpu10I CCTNAGC 1 cut(s) 280
Bsa29I ATCGAT 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 63, 69
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 2 cut(s) 213, 496
BseCI ATCGAT 1 cut(s) 89
BseDI CCNNGG 2 cut(s) 63, 69
BseGI GGATG 1 cut(s) 385
BseLI CCNNNNNNNGG 2 cut(s) 213, 496
BseMII CTCAG 3 cut(s) 226, 294, 501
BseXI GCAGC 1 cut(s) 7
Bsh1236I CGCG 1 cut(s) 48
BshFI GGCC 1 cut(s) 68
BshVI ATCGAT 1 cut(s) 89
BslFI GGGAC 2 cut(s) 492, 507
BslI CCNNNNNNNGG 2 cut(s) 213, 496
BsmFI GGGAC 2 cut(s) 492, 507
BsmI GAATGC 1 cut(s) 536
BsnI GGCC 1 cut(s) 68
Bsp143I GATC 5 cut(s) 86, 136, 153, 354, 463
Bsp19I CCATGG 1 cut(s) 69
Bsp68I TCGCGA 1 cut(s) 48
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 3 cut(s) 225, 293, 502
BspDI ATCGAT 1 cut(s) 89
BspFNI CGCG 1 cut(s) 48
BspPI GGATC 3 cut(s) 94, 131, 362
BssECI CCNNGG 2 cut(s) 63, 69
BssMI GATC 5 cut(s) 86, 136, 153, 354, 463
BssNAI GTATAC 1 cut(s) 234
BssSI CACGAG 1 cut(s) 57
BssT1I CCWWGG 2 cut(s) 63, 69
Bst1107I GTATAC 1 cut(s) 234
Bst2BI CACGAG 1 cut(s) 57
Bst6I CTCTTC 2 cut(s) 117, 291
BstC8I GCNNGC 1 cut(s) 46
BstDEI CTNAG 3 cut(s) 212, 280, 510
BstDSI CCRYGG 1 cut(s) 69
BstF5I GGATG 1 cut(s) 385
BstFNI CGCG 1 cut(s) 48
BstKTI GATC 5 cut(s) 89, 139, 156, 357, 466
BstMBI GATC 5 cut(s) 86, 136, 153, 354, 463
BstSFI CTRYAG 1 cut(s) 183
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 1 cut(s) 7
BstXI CCANNNNNNTGG 1 cut(s) 70
BstZ17I GTATAC 1 cut(s) 234
Bsu15I ATCGAT 1 cut(s) 89
BsuI GTATCC 1 cut(s) 100
BsuRI GGCC 1 cut(s) 68
BsuTUI ATCGAT 1 cut(s) 89
BtgI CCRYGG 1 cut(s) 69
BtgZI GCGATG 1 cut(s) 63
BtsCI GGATG 1 cut(s) 385
BtuMI TCGCGA 1 cut(s) 48
Cac8I GCNNGC 1 cut(s) 46
ClaI ATCGAT 1 cut(s) 89
CviAII CATG 2 cut(s) 70, 406
CviJI RGCY 8 cut(s) 20, 44, 68, 148, 225, 258, 279, 422
CviKI_1 RGCY 8 cut(s) 20, 44, 68, 148, 225, 258, 279, 422
DdeI CTNAG 3 cut(s) 212, 280, 510
DpnI GATC 5 cut(s) 88, 138, 155, 356, 465
DpnII GATC 5 cut(s) 86, 136, 153, 354, 463
Eam1104I CTCTTC 2 cut(s) 117, 291
EarI CTCTTC 2 cut(s) 117, 291
Eco130I CCWWGG 2 cut(s) 63, 69
EcoT14I CCWWGG 2 cut(s) 63, 69
EcoT22I ATGCAT 2 cut(s) 293, 538
ErhI CCWWGG 2 cut(s) 63, 69
FaeI CATG 2 cut(s) 73, 409
FaqI GGGAC 2 cut(s) 492, 507
FatI CATG 2 cut(s) 69, 405
FblI GTMKAC 1 cut(s) 233
Fnu4HI GCNGC 1 cut(s) 21
FokI GGATG 1 cut(s) 392
Fsp4HI GCNGC 1 cut(s) 21
FspBI CTAG 2 cut(s) 102, 273
GluI GCNGC 1 cut(s) 21
HaeIII GGCC 1 cut(s) 68
Hin1II CATG 2 cut(s) 73, 409
HindIII AAGCTT 1 cut(s) 420
HinfI GANTC 1 cut(s) 91
Hpy166II GTNNAC 1 cut(s) 234
Hpy188I TCNGA 1 cut(s) 145
Hpy188III TCNNGA 5 cut(s) 47, 116, 282, 304, 477
Hpy8I GTNNAC 1 cut(s) 234
HpyAV CCTTC 1 cut(s) 242
HpyCH4IV ACGT 3 cut(s) 78, 430, 481
HpyCH4V TGCA 2 cut(s) 291, 536
HpyF3I CTNAG 3 cut(s) 212, 280, 510
HpySE526I ACGT 3 cut(s) 78, 430, 481
Hsp92II CATG 2 cut(s) 73, 409
Kzo9I GATC 5 cut(s) 86, 136, 153, 354, 463
LmnI GCTCC 1 cut(s) 17
LpnPI CCDG 3 cut(s) 199, 267, 377
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 1 cut(s) 523
MaeI CTAG 2 cut(s) 102, 273
MaeII ACGT 3 cut(s) 78, 430, 481
MaeIII GTNAC 1 cut(s) 482
MalI GATC 5 cut(s) 88, 138, 155, 356, 465
MboI GATC 5 cut(s) 86, 136, 153, 354, 463
MboII GAAGA 3 cut(s) 104, 308, 323
MfeI CAATTG 1 cut(s) 517
MluCI AATT 2 cut(s) 190, 517
MmeI TCCRAC 1 cut(s) 119
MnlI CCTC 5 cut(s) 3, 66, 215, 370, 500
Mph1103I ATGCAT 2 cut(s) 293, 538
MseI TTAA 2 cut(s) 176, 372
MslI CAYNNNNRTG 1 cut(s) 531
MunI CAATTG 1 cut(s) 517
Mva1269I GAATGC 1 cut(s) 536
MvnI CGCG 1 cut(s) 48
NcoI CCATGG 1 cut(s) 69
NdeII GATC 5 cut(s) 86, 136, 153, 354, 463
NlaIII CATG 2 cut(s) 73, 409
NruI TCGCGA 1 cut(s) 48
NsiI ATGCAT 2 cut(s) 293, 538
PctI GAATGC 1 cut(s) 536
PfeI GAWTC 1 cut(s) 91
PkrI GCNGC 1 cut(s) 22
PsiI TTATAA 1 cut(s) 399
RruI TCGCGA 1 cut(s) 48
RseI CAYNNNNRTG 1 cut(s) 531
SaqAI TTAA 2 cut(s) 176, 372
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 5 cut(s) 86, 136, 153, 354, 463
SfaNI GCATC 1 cut(s) 523
SfcI CTRYAG 1 cut(s) 183
SmiMI CAYNNNNRTG 1 cut(s) 531
Sse9I AATT 2 cut(s) 190, 517
SspMI CTAG 2 cut(s) 102, 273
StyI CCWWGG 2 cut(s) 63, 69
TaiI ACGT 3 cut(s) 81, 433, 484
TaqI TCGA 2 cut(s) 89, 115
TasI AATT 2 cut(s) 190, 517
TfiI GAWTC 1 cut(s) 91
Tru1I TTAA 2 cut(s) 176, 372
Tru9I TTAA 2 cut(s) 176, 372
TseI GCWGC 1 cut(s) 20
TspDTI ATGAA 2 cut(s) 394, 529
XmiI GTMKAC 1 cut(s) 233
XspI CTAG 2 cut(s) 102, 273
Zsp2I ATGCAT 2 cut(s) 293, 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.