Rroxscaffold_1G00070330

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0025002
Physical Location & Seq
Forward (+)
91210315 .. 91228769
18455 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00070330.1

Sequence Viewer

Length: 747 bp
ATGATCAGGTTGCTCGGCTACTGTGACTATAGATGTGACTTGGCTTTTCATATTTCTGGTTCAAGGGTTTGCTGGGAGACGACGAACAAGGATCGACAAATAAACGATTGGCCTTTTGTATTTCTGGGGTATTGTAAGAGTTCTGTTACGTTTTGGATATGTCCAGGGACTTCATCCCAATGTTTGTGTTTTCAGAGTTGTCAGAAGGTTTTGTTGATCGCCAGACTTCAACCCAGAAATTGCTCTAGTATTATAAAAAAGGTGTCCAGCTCGATGGAAACCCTAGAATTTCAAAGCTTCGATTCGTCCTCCACAAGTGAAATCGCTCCAAGCCACTTTGGGAAAAGTATCTACGTCCTCCGAGCTCCAAAAGCTCTCAAGAATCACCGGCAAAGAGCCGAGCTTCTCCATGTTTTCGATGTGGGACGATGCGCTTTAGTCCCCGCCGGAGCTTCTCGCCGCTCTCTTGGCGAGGCGAGTGGCGGCGCGTCGGCGGTGATCCGGGGTGAGCCGGGCTCGTGCCGTAGCCCGCCTGGCCATCCAATGAAGAGAGCAATGCTTGATTGGGCTACGCGCTTTTATATTATTCAGGATATTGCTAGAAAGCTTCTTTATCTCCATCATGATTCCTCCGTGAAGGTGATACATAGAGATTTAAAAGTCAGTAACATTCTCTTGGATGAGAAAATGAATCCAAAAATCTCAGATTTTGGATTTGCACGTATAGTTGAAGAAACACGAGTCTAG

Protein Analysis

248

Amino Acids

27.93

Weight (kDa)

9.33

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 185 - 245 8.9e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 195 - 246 1.5e-12 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0025002)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0005401 RchiOBHm_Chr5g0005481
rosa_roxburghii Rroxscaffold_1G00070330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 254
AccBSI CCGCTC 1 cut(s) 462
AccII CGCG 2 cut(s) 488, 574
AciI CCGC 5 cut(s) 444, 460, 483, 494, 530
AclWI GGATC 2 cut(s) 99, 493
AcoI YGGCCR 1 cut(s) 535
AcsI RAATTY 1 cut(s) 287
AgsI TTSAA 4 cut(s) 63, 230, 293, 731
AjnI CCWGG 2 cut(s) 163, 532
AluBI AGCT 7 cut(s) 270, 297, 365, 374, 403, 452, 607
AluI AGCT 7 cut(s) 270, 297, 365, 374, 403, 452, 607
Alw21I GWGCWC 1 cut(s) 367
Alw26I GTCTC 1 cut(s) 71
AlwI GGATC 2 cut(s) 99, 493
AoxI GGCC 2 cut(s) 110, 535
ApoI RAATTY 1 cut(s) 287
AspLEI GCGC 3 cut(s) 434, 488, 576
AsuC2I CCSGG 2 cut(s) 503, 513
AsuHPI GGTGA 4 cut(s) 377, 508, 518, 652
BalI TGGCCA 1 cut(s) 537
BanII GRGCYC 2 cut(s) 367, 518
BauI CACGAG 2 cut(s) 517, 738
Bbv12I GWGCWC 1 cut(s) 367
BccI CCATC 3 cut(s) 268, 546, 627
BceAI ACGGC 1 cut(s) 507
BciT130I CCWGG 2 cut(s) 165, 534
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 2 cut(s) 503, 513
BcoDI GTCTC 1 cut(s) 71
BfaI CTAG 4 cut(s) 246, 284, 600, 745
BfmI CTRYAG 1 cut(s) 28
BglI GCCNNNNNGGC 1 cut(s) 534
BisI GCNGC 2 cut(s) 460, 484
BlsI GCNGC 2 cut(s) 461, 485
Bme1390I CCNGG 4 cut(s) 165, 503, 513, 534
BmrFI CCNGG 4 cut(s) 165, 503, 513, 534
BmsI GCATC 1 cut(s) 419
BplI GAGNNNNNCTC 2 cut(s) 500, 532
BpuEI CTTGAG 1 cut(s) 362
BpuMI CCSGG 2 cut(s) 503, 513
BsaAI YACGTR 1 cut(s) 722
BsaJI CCNNGG 2 cut(s) 164, 502
Bse118I RCCGGY 1 cut(s) 387
Bse3DI GCAATG 1 cut(s) 561
BseBI CCWGG 2 cut(s) 165, 534
BseDI CCNNGG 2 cut(s) 164, 502
BseGI GGATG 3 cut(s) 173, 538, 685
BseMI GCAATG 1 cut(s) 561
BseMII CTCAG 1 cut(s) 717
BseYI CCCAGC 1 cut(s) 72
Bsh1236I CGCG 2 cut(s) 488, 574
BshFI GGCC 2 cut(s) 112, 537
BsiHKAI GWGCWC 1 cut(s) 367
BsiSI CCGG 4 cut(s) 388, 447, 502, 512
BslFI GGGAC 3 cut(s) 181, 425, 438
BsmAI GTCTC 1 cut(s) 71
BsmBI CGTCTC 1 cut(s) 71
BsmFI GGGAC 3 cut(s) 181, 425, 438
BsnI GGCC 2 cut(s) 112, 537
Bsp1286I GDGCHC 2 cut(s) 367, 518
Bsp143I GATC 4 cut(s) 3, 91, 216, 498
BspACI CCGC 5 cut(s) 444, 460, 483, 494, 530
BspANI GGCC 2 cut(s) 112, 537
BspCNI CTCAG 1 cut(s) 716
BspFNI CGCG 2 cut(s) 488, 574
BspHI TCATGA 1 cut(s) 622
BspPI GGATC 2 cut(s) 99, 493
BsrBI CCGCTC 1 cut(s) 462
BsrDI GCAATG 1 cut(s) 561
BsrFI RCCGGY 1 cut(s) 387
BssAI RCCGGY 1 cut(s) 387
BssECI CCNNGG 2 cut(s) 164, 502
BssMI GATC 4 cut(s) 3, 91, 216, 498
BssSI CACGAG 2 cut(s) 517, 738
Bst2BI CACGAG 2 cut(s) 517, 738
Bst2UI CCWGG 2 cut(s) 165, 534
Bst4CI ACNGT 1 cut(s) 23
Bst6I CTCTTC 1 cut(s) 542
BstBAI YACGTR 1 cut(s) 722
BstC8I GCNNGC 1 cut(s) 530
BstDEI CTNAG 1 cut(s) 703
BstF5I GGATG 3 cut(s) 173, 538, 685
BstFNI CGCG 2 cut(s) 488, 574
BstHHI GCGC 3 cut(s) 434, 488, 576
BstKTI GATC 4 cut(s) 6, 94, 219, 501
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 4 cut(s) 3, 91, 216, 498
BstMWI GCNNNNNNNGC 3 cut(s) 371, 468, 534
BstNI CCWGG 2 cut(s) 165, 534
BstSCI CCNGG 4 cut(s) 163, 501, 511, 532
BstSFI CTRYAG 1 cut(s) 28
BstUI CGCG 2 cut(s) 488, 574
BstXI CCANNNNNNTGG 1 cut(s) 274
BsuRI GGCC 2 cut(s) 112, 537
BtsCI GGATG 3 cut(s) 173, 538, 685
Cac8I GCNNGC 1 cut(s) 530
CciI TCATGA 1 cut(s) 622
CfoI GCGC 3 cut(s) 434, 488, 576
Cfr10I RCCGGY 1 cut(s) 387
CseI GACGC 1 cut(s) 477
CviAII CATG 2 cut(s) 410, 623
DdeI CTNAG 1 cut(s) 703
DpnI GATC 4 cut(s) 5, 93, 218, 500
DpnII GATC 4 cut(s) 3, 91, 216, 498
DraI TTTAAA 1 cut(s) 657
EaeI YGGCCR 1 cut(s) 535
Eam1104I CTCTTC 1 cut(s) 542
EarI CTCTTC 1 cut(s) 542
Ecl136II GAGCTC 1 cut(s) 365
Eco24I GRGCYC 2 cut(s) 367, 518
Eco53kI GAGCTC 1 cut(s) 365
EcoICRI GAGCTC 1 cut(s) 365
EcoRII CCWGG 2 cut(s) 163, 532
EcoT38I GRGCYC 2 cut(s) 367, 518
Esp3I CGTCTC 1 cut(s) 71
FaeI CATG 2 cut(s) 413, 626
FaiI YATR 9 cut(s) 30, 51, 160, 254, 411, 582, 624, 648, 725
FaqI GGGAC 3 cut(s) 181, 425, 438
FatI CATG 2 cut(s) 409, 622
FauI CCCGC 2 cut(s) 451, 537
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 2 cut(s) 460, 484
FokI GGATG 3 cut(s) 160, 525, 692
FriOI GRGCYC 2 cut(s) 367, 518
Fsp4HI GCNGC 2 cut(s) 460, 484
FspBI CTAG 4 cut(s) 246, 284, 600, 745
GlaI GCGC 3 cut(s) 433, 487, 575
GluI GCNGC 2 cut(s) 460, 484
GsaI CCCAGC 1 cut(s) 76
HaeIII GGCC 2 cut(s) 112, 537
HapII CCGG 4 cut(s) 388, 447, 502, 512
HgaI GACGC 1 cut(s) 477
HhaI GCGC 3 cut(s) 434, 488, 576
Hin1II CATG 2 cut(s) 413, 626
Hin6I GCGC 3 cut(s) 432, 486, 574
HinP1I GCGC 3 cut(s) 432, 486, 574
HindIII AAGCTT 2 cut(s) 295, 605
HinfI GANTC 5 cut(s) 302, 382, 626, 691, 741
HpaII CCGG 4 cut(s) 388, 447, 502, 512
HphI GGTGA 4 cut(s) 377, 508, 518, 652
Hpy188I TCNGA 4 cut(s) 195, 204, 362, 706
Hpy188III TCNNGA 3 cut(s) 379, 590, 623
Hpy99I CGWCG 2 cut(s) 85, 493
HpyAV CCTTC 2 cut(s) 199, 631
HpyCH4III ACNGT 1 cut(s) 23
HpyCH4IV ACGT 3 cut(s) 149, 354, 721
HpyCH4V TGCA 1 cut(s) 719
HpyF10VI GCNNNNNNNGC 3 cut(s) 371, 468, 534
HpyF3I CTNAG 1 cut(s) 703
HpySE526I ACGT 3 cut(s) 149, 354, 721
Hsp92II CATG 2 cut(s) 413, 626
HspAI GCGC 3 cut(s) 432, 486, 574
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 4 cut(s) 3, 91, 216, 498
LmnI GCTCC 3 cut(s) 331, 370, 449
LweI GCATC 1 cut(s) 419
MaeI CTAG 4 cut(s) 246, 284, 600, 745
MaeII ACGT 3 cut(s) 149, 354, 721
MaeIII GTNAC 4 cut(s) 23, 35, 145, 665
MalI GATC 4 cut(s) 5, 93, 218, 500
MbiI CCGCTC 1 cut(s) 462
MboI GATC 4 cut(s) 3, 91, 216, 498
MboII GAAGA 2 cut(s) 559, 743
MhlI GDGCHC 2 cut(s) 367, 518
MlsI TGGCCA 1 cut(s) 537
MluCI AATT 2 cut(s) 238, 287
MluNI TGGCCA 1 cut(s) 537
MnlI CCTC 4 cut(s) 319, 368, 466, 640
Mox20I TGGCCA 1 cut(s) 537
MscI TGGCCA 1 cut(s) 537
MseI TTAA 1 cut(s) 656
MslI CAYNNNNRTG 1 cut(s) 178
Msp20I TGGCCA 1 cut(s) 537
MspI CCGG 4 cut(s) 388, 447, 502, 512
MspR9I CCNGG 4 cut(s) 165, 503, 513, 534
MvaI CCWGG 2 cut(s) 165, 534
MvnI CGCG 2 cut(s) 488, 574
MwoI GCNNNNNNNGC 3 cut(s) 371, 468, 534
NciI CCSGG 2 cut(s) 503, 513
NdeII GATC 4 cut(s) 3, 91, 216, 498
NlaIII CATG 2 cut(s) 413, 626
NmeAIII GCCGAG 1 cut(s) 424
NmuCI GTSAC 2 cut(s) 23, 35
PagI TCATGA 1 cut(s) 622
PfeI GAWTC 4 cut(s) 302, 382, 626, 691
PkrI GCNGC 2 cut(s) 461, 485
Ppu21I YACGTR 1 cut(s) 722
PsiI TTATAA 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 367
Psp6I CCWGG 2 cut(s) 163, 532
PspFI CCCAGC 1 cut(s) 72
PspGI CCWGG 2 cut(s) 163, 532
RseI CAYNNNNRTG 1 cut(s) 178
SacI GAGCTC 1 cut(s) 367
SaqAI TTAA 1 cut(s) 656
SatI GCNGC 2 cut(s) 460, 484
Sau3AI GATC 4 cut(s) 3, 91, 216, 498
ScrFI CCNGG 4 cut(s) 165, 503, 513, 534
SduI GDGCHC 2 cut(s) 367, 518
SfaNI GCATC 1 cut(s) 419
SfcI CTRYAG 1 cut(s) 28
SmiMI CAYNNNNRTG 1 cut(s) 178
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 2 cut(s) 238, 287
SsiI CCGC 5 cut(s) 444, 460, 483, 494, 530
SspMI CTAG 4 cut(s) 246, 284, 600, 745
SstI GAGCTC 1 cut(s) 367
StyD4I CCNGG 4 cut(s) 163, 501, 511, 532
TaaI ACNGT 1 cut(s) 23
TaiI ACGT 3 cut(s) 152, 357, 724
TaqI TCGA 4 cut(s) 94, 272, 300, 417
TasI AATT 2 cut(s) 238, 287
TauI GCSGC 2 cut(s) 462, 486
TfiI GAWTC 4 cut(s) 302, 382, 626, 691
Tru1I TTAA 1 cut(s) 656
Tru9I TTAA 1 cut(s) 656
TseFI GTSAC 2 cut(s) 23, 35
Tsp45I GTSAC 2 cut(s) 23, 35
TspDTI ATGAA 4 cut(s) 38, 162, 560, 704
TspGWI ACGGA 1 cut(s) 622
XapI RAATTY 1 cut(s) 287
XspI CTAG 4 cut(s) 246, 284, 600, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.