Rroxscaffold_2G00082180

Glucose / Sorbosone dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0017056
Physical Location & Seq
Reverse (-)
5045736 .. 5049690
3955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00082180.1

Sequence Viewer

Length: 1656 bp
ATGACGCACCGCCCGGTGACAGTGGAAACATTTTTTATTTCTATAGTTGCTGATTGGTTTCTTTGCCCTCTGCGTCAATATTGGAATAATATAGGGTCACCCTTCAAGCCAAAGACCCCACTTTCGTTCTGTCAATACAATGGAAGTGTTTGCTGTAATTCCACTGATGATATGCAATTGCAAACTCTATTTAAGGCTATGAATGTTTCTGATCTCAGTTGTGCTTCTCTTCTGAAATCTACACTTTGCTCGAGATGTGATCAGTTTTCCGCAGATCTATACAGAACGGAATCAGTGCCTCGTGAAGTTCCTGTTCTCTGCAACTCAACTGTTTCATCAAACTCAACCCAATACCAACACAGTGGAGCTGACTTTTGTACCAAGGTTTGGGATGAATGCCAAAATGTATCTGTATTGAGTTCTCCATTTGCATTGCAAGGTAGAGGTCGAACACCATTTAATTCTACTTCCAAGCTGAGTGATATATGGGAGTCAAAAAGTGCTTTCTGTGATACATTTGGTGGATCTTCTGATGAGGAATTGGTCTGTTTTGACGGTGGACCTGTGTTGCTGAACAACACTGAAATTCCAAGTCCCCCAAGTGGCATGTGCCTTGAAAAAATTGGAAATGGATCTTATCTTAACATGGTACCTCATCCAGATGGGTCTAATCGCGTGTTCTTATCTGATCAACCGGGGAAAGTATGGCTGGCAACCGTTCCTGAGGAAGGATCAGGAGAAATGTTGGTGATTGATGATTCAAATCCGTTTCTTGATCTTACTGATCAAGTGTATGCTGACACTGAGTTTGGAATGATGGGATTAGCATTCCATCCTAATTATCTACAGAATGGCCGTTTCTTTGCTTCATTTAACTGTGATAAGGTTAGATGGCCAGAATGTTCAGGAAGATGTTCATGCAACTCAGATGTGGGATGTGATCCTTCCAAGCTTGGTTCTGATAATGGTGCTCAGCCATGTCAATATCACAGTATCATAGCAGAGTTCAGTGCTAATGGGACCGCATCACAACCTTCTTCGGTCACTAGTGTTAAACCATTGGAAGTCAGAAGAATTTTCACTATGGGACTTCCATTTACTTCCCATCATGGAGGTCAAATTCTTTTTGGACCCGAAGACGGGTATTTGTACTTCATGATGGGAGATGGTGGTAGCGACAAATATGAGGAGGTTGATATAGTCACCAAGGGTGGAAACTACGGATGGCGTGCATATGAGGGTGCTTTTCCATACCATCCCCCAAAGTCTCCAGGAGGAAATACATCTACGAGTTCTATCAACCCGATTTTCCCTGTAATGGGATACAACCACTCTGATGTCAACAAGGCTGAAGGTTCTGCATCAATCACTGGTGGCTACTTTTACCGGTCCATGACAGATCCATGCATGTATGGAAGGTATCTTTATGCGGATTTGTATGCTGGTCACATATGGGCAGGTACAGAAACTCCTGAAGATAGCGGAAACTTCACAAGTACTATAGTTCCTGTCAGTTGTGCTCTGGACTCTCCTATCCAGTGCAGAGCAGAGGCAGGAAGTTCTTTTCCTGCATTGGGGTTCATATTCTCCTTTGGTCAGGATAACAGGAAAGACATGCTCTGCTCCTTCTCCTTCTCCCTCCACTTCAAGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GSDH

Protein Analysis

551

Amino Acids

60.38

Weight (kDa)

4.88

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017056)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G39970 AT5G39970 AT5G39970
fragaria_vesca FvH4_6g49480
malus_domestica MD17G1046100.v1.1
prunus_persica Prupe.3G273500_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0169421
rosa_laevigata RLG00000021860
rosa_multiflora Rmu_sc0000648.1_g000012
rosa_roxburghii Rroxscaffold_2G00082180
rosa_rugosa Rorug02G0539700
rosa_samantha Rh2BG624600
rosa_wichuraiana Rw2G050700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1448
Acc65I GGTACC 1 cut(s) 649
AccB1I GGYRCC 1 cut(s) 649
AccB7I CCANNNNNTGG 1 cut(s) 387
AccII CGCG 1 cut(s) 675
AciI CCGC 5 cut(s) 10, 270, 1023, 1430, 1482
AclWI GGATC 5 cut(s) 532, 640, 739, 935, 1394
AcoI YGGCCR 2 cut(s) 853, 893
AcsI RAATTY 3 cut(s) 585, 1074, 1119
AcuI CTGAAG 2 cut(s) 1371, 1494
AfaI GTAC 5 cut(s) 379, 651, 1151, 1462, 1498
AfiI CCNNNNNNNGG 8 cut(s) 387, 602, 728, 1139, 1140, 1318, 1319, 1574
AgeI ACCGGT 1 cut(s) 1386
AgsI TTSAA 4 cut(s) 106, 617, 762, 1648
AhlI ACTAGT 1 cut(s) 1046
AjnI CCWGG 1 cut(s) 1270
AluBI AGCT 3 cut(s) 368, 475, 952
AluI AGCT 3 cut(s) 368, 475, 952
Alw21I GWGCWC 2 cut(s) 973, 1522
Alw26I GTCTC 1 cut(s) 1272
AlwI GGATC 5 cut(s) 532, 640, 739, 935, 1394
Ama87I CYCGRG 1 cut(s) 250
AoxI GGCC 2 cut(s) 853, 893
ApoI RAATTY 3 cut(s) 585, 1074, 1119
ArsI GACNNNNNNTTYG 4 cut(s) 106, 138, 791, 823
AsiGI ACCGGT 1 cut(s) 1386
Asp700I GAANNNNTTC 1 cut(s) 913
Asp718I GGTACC 1 cut(s) 649
AspS9I GGNCC 4 cut(s) 560, 1020, 1130, 1389
AsuC2I CCSGG 2 cut(s) 14, 696
AsuHPI GGTGA 4 cut(s) 28, 90, 760, 1195
AvaI CYCGRG 1 cut(s) 250
AvaII GGWCC 4 cut(s) 560, 1020, 1130, 1389
AxyI CCTNAGG 1 cut(s) 723
BaeI ACNNNNGTAYC 2 cut(s) 361, 394
BalI TGGCCA 1 cut(s) 895
BanI GGYRCC 1 cut(s) 649
BarI GAAGNNNNNNTAC 2 cut(s) 1136, 1168
BauI CACGAG 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 1143
Bbv12I GWGCWC 2 cut(s) 973, 1522
BccI CCATC 9 cut(s) 656, 811, 840, 885, 1113, 1153, 1160, 1218, 1263
BceAI ACGGC 1 cut(s) 840
BciT130I CCWGG 1 cut(s) 1272
BciVI GTATCC 1 cut(s) 1316
BclI TGATCA 3 cut(s) 259, 688, 784
BcnI CCSGG 2 cut(s) 14, 696
BcoDI GTCTC 1 cut(s) 1272
BcuI ACTAGT 1 cut(s) 1046
BfaI CTAG 2 cut(s) 1047, 1654
BfmI CTRYAG 3 cut(s) 42, 845, 1500
BfuAI ACCTGC 1 cut(s) 1448
BfuI GTATCC 1 cut(s) 1316
BglII AGATCT 1 cut(s) 274
BlpI GCTNAGC 1 cut(s) 972
BmcAI AGTACT 1 cut(s) 1498
Bme1390I CCNGG 3 cut(s) 14, 696, 1272
Bme18I GGWCC 4 cut(s) 560, 1020, 1130, 1389
BmeT110I CYCGRG 1 cut(s) 250
BmgT120I GGNCC 4 cut(s) 560, 1020, 1130, 1389
BmiI GGNNCC 3 cut(s) 651, 1021, 1132
BmrFI CCNGG 3 cut(s) 14, 696, 1272
BmsI GCATC 2 cut(s) 1034, 1370
BpiI GAAGAC 1 cut(s) 1143
BpmI CTGGAG 1 cut(s) 1254
Bpu1102I GCTNAGC 1 cut(s) 972
BpuMI CCSGG 2 cut(s) 14, 696
BsaBI GATNNNNATC 1 cut(s) 762
BsaJI CCNNGG 3 cut(s) 381, 695, 1206
BsaWI WCCGGW 1 cut(s) 1386
BsaXI ACNNNNNCTCC 2 cut(s) 1453, 1483
Bsc4I CCNNNNNNNGG 8 cut(s) 387, 602, 728, 1139, 1140, 1318, 1319, 1574
Bse118I RCCGGY 1 cut(s) 1386
Bse1I ACTGG 2 cut(s) 1375, 1537
Bse21I CCTNAGG 1 cut(s) 723
Bse3DI GCAATG 1 cut(s) 431
Bse8I GATNNNNATC 1 cut(s) 762
BseBI CCWGG 1 cut(s) 1272
BseDI CCNNGG 3 cut(s) 381, 695, 1206
BseGI GGATG 6 cut(s) 397, 655, 832, 941, 1229, 1255
BseJI GATNNNNATC 1 cut(s) 762
BseLI CCNNNNNNNGG 8 cut(s) 387, 602, 728, 1139, 1140, 1318, 1319, 1574
BseMI GCAATG 1 cut(s) 431
BseMII CTCAG 6 cut(s) 229, 467, 714, 795, 939, 986
BseNI ACTGG 2 cut(s) 1375, 1537
BseRI GAGGAG 1 cut(s) 1202
BsgI GTGCAG 1 cut(s) 1561
Bsh1236I CGCG 1 cut(s) 675
BshFI GGCC 2 cut(s) 855, 895
BshNI GGYRCC 1 cut(s) 649
BshTI ACCGGT 1 cut(s) 1386
BsiHKAI GWGCWC 2 cut(s) 973, 1522
BsiHKCI CYCGRG 1 cut(s) 250
BsiSI CCGG 3 cut(s) 14, 695, 1387
BslFI GGGAC 3 cut(s) 579, 1033, 1101
BslI CCNNNNNNNGG 8 cut(s) 387, 602, 728, 1139, 1140, 1318, 1319, 1574
BsmAI GTCTC 1 cut(s) 1272
BsmFI GGGAC 3 cut(s) 579, 1033, 1101
BsmI GAATGC 2 cut(s) 401, 827
BsnI GGCC 2 cut(s) 855, 895
BsoBI CYCGRG 1 cut(s) 250
Bsp1286I GDGCHC 2 cut(s) 973, 1522
Bsp1720I GCTNAGC 1 cut(s) 972
BspACI CCGC 5 cut(s) 10, 270, 1023, 1430, 1482
BspANI GGCC 2 cut(s) 855, 895
BspCNI CTCAG 6 cut(s) 228, 468, 715, 796, 938, 985
BspFNI CGCG 1 cut(s) 675
BspHI TCATGA 1 cut(s) 1155
BspLI GGNNCC 3 cut(s) 651, 1021, 1132
BspMI ACCTGC 1 cut(s) 1448
BspPI GGATC 5 cut(s) 532, 640, 739, 935, 1394
BspT107I GGYRCC 1 cut(s) 649
BsrDI GCAATG 1 cut(s) 431
BsrFI RCCGGY 1 cut(s) 1386
BsrI ACTGG 2 cut(s) 1375, 1537
BssAI RCCGGY 1 cut(s) 1386
BssECI CCNNGG 3 cut(s) 381, 695, 1206
BssSI CACGAG 1 cut(s) 300
BssT1I CCWWGG 2 cut(s) 381, 1206
Bst2BI CACGAG 1 cut(s) 300
Bst2UI CCWGG 1 cut(s) 1272
Bst4CI ACNGT 7 cut(s) 22, 331, 362, 557, 718, 878, 992
Bst6I CTCTTC 1 cut(s) 234
BstC8I GCNNGC 2 cut(s) 711, 1230
BstDEI CTNAG 6 cut(s) 215, 476, 723, 804, 925, 972
BstEII GGTNACC 1 cut(s) 96
BstENI CCTNNNNNAGG 1 cut(s) 726
BstF5I GGATG 6 cut(s) 397, 655, 832, 941, 1229, 1255
BstFNI CGCG 1 cut(s) 675
BstMAI GTCTC 1 cut(s) 1272
BstNI CCWGG 1 cut(s) 1272
BstNSI RCATGY 3 cut(s) 610, 1411, 1618
BstPI GGTNACC 1 cut(s) 96
BstSCI CCNGG 3 cut(s) 12, 694, 1270
BstSFI CTRYAG 3 cut(s) 42, 845, 1500
BstUI CGCG 1 cut(s) 675
BstV2I GAAGAC 1 cut(s) 1143
BstX2I RGATCY 4 cut(s) 274, 524, 632, 1399
BstXI CCANNNNNNTGG 1 cut(s) 362
BstYI RGATCY 4 cut(s) 274, 524, 632, 1399
Bsu36I CCTNAGG 1 cut(s) 723
BsuI GTATCC 1 cut(s) 1316
BsuRI GGCC 2 cut(s) 855, 895
BtsCI GGATG 6 cut(s) 397, 655, 832, 941, 1229, 1255
BtsIMutI CAGTG 9 cut(s) 27, 162, 300, 367, 579, 801, 1015, 1368, 1544
BveI ACCTGC 1 cut(s) 1448
Cac8I GCNNGC 2 cut(s) 711, 1230
CciI TCATGA 1 cut(s) 1155
Cfr10I RCCGGY 1 cut(s) 1386
Cfr13I GGNCC 4 cut(s) 560, 1020, 1130, 1389
CseI GACGC 2 cut(s) 13, 62
Csp6I GTAC 5 cut(s) 378, 650, 1150, 1461, 1497
CspAI ACCGGT 1 cut(s) 1386
CviQI GTAC 5 cut(s) 378, 650, 1150, 1461, 1497
DdeI CTNAG 6 cut(s) 215, 476, 723, 804, 925, 972
EaeI YGGCCR 2 cut(s) 853, 893
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Eco130I CCWWGG 2 cut(s) 381, 1206
Eco47I GGWCC 4 cut(s) 560, 1020, 1130, 1389
Eco57I CTGAAG 2 cut(s) 1371, 1494
Eco81I CCTNAGG 1 cut(s) 723
Eco88I CYCGRG 1 cut(s) 250
Eco91I GGTNACC 1 cut(s) 96
EcoNI CCTNNNNNAGG 1 cut(s) 726
EcoO65I GGTNACC 1 cut(s) 96
EcoRII CCWGG 1 cut(s) 1270
EcoT14I CCWWGG 2 cut(s) 381, 1206
EcoT22I ATGCAT 1 cut(s) 1409
ErhI CCWWGG 2 cut(s) 381, 1206
FaqI GGGAC 3 cut(s) 579, 1033, 1101
FauNDI CATATG 2 cut(s) 1234, 1451
FbaI TGATCA 3 cut(s) 259, 688, 784
FokI GGATG 6 cut(s) 404, 642, 819, 948, 1236, 1242
FspBI CTAG 2 cut(s) 1047, 1654
GsuI CTGGAG 1 cut(s) 1254
HaeIII GGCC 2 cut(s) 855, 895
HapII CCGG 3 cut(s) 14, 695, 1387
HgaI GACGC 2 cut(s) 13, 62
HincII GTYRAC 1 cut(s) 1342
HindII GTYRAC 1 cut(s) 1342
HindIII AAGCTT 1 cut(s) 950
HinfI GANTC 4 cut(s) 290, 491, 758, 1526
HpaII CCGG 3 cut(s) 14, 695, 1387
HphI GGTGA 4 cut(s) 28, 90, 760, 1195
Hpy166II GTNNAC 2 cut(s) 560, 1342
Hpy188I TCNGA 8 cut(s) 211, 234, 532, 688, 928, 961, 1070, 1336
Hpy8I GTNNAC 2 cut(s) 560, 1342
HpyAV CCTTC 8 cut(s) 112, 722, 954, 1044, 1346, 1410, 1636, 1642
HpyCH4III ACNGT 7 cut(s) 22, 331, 362, 557, 718, 878, 992
HpyF3I CTNAG 6 cut(s) 215, 476, 723, 804, 925, 972
KpnI GGTACC 1 cut(s) 653
Ksp22I TGATCA 3 cut(s) 259, 688, 784
LmnI GCTCC 2 cut(s) 365, 1628
LweI GCATC 2 cut(s) 1034, 1370
MaeI CTAG 2 cut(s) 1047, 1654
MaeIII GTNAC 5 cut(s) 16, 96, 1042, 1201, 1445
MboII GAAGA 7 cut(s) 221, 519, 921, 1029, 1083, 1148, 1487
MfeI CAATTG 1 cut(s) 176
MflI RGATCY 4 cut(s) 274, 524, 632, 1399
MhlI GDGCHC 2 cut(s) 973, 1522
MlsI TGGCCA 1 cut(s) 895
MluCI AATT 9 cut(s) 157, 176, 460, 539, 585, 621, 838, 1074, 1119
MluNI TGGCCA 1 cut(s) 895
MlyI GAGTC 2 cut(s) 500, 1520
Mox20I TGGCCA 1 cut(s) 895
Mph1103I ATGCAT 1 cut(s) 1409
MroXI GAANNNNTTC 1 cut(s) 913
MscI TGGCCA 1 cut(s) 895
MseI TTAA 5 cut(s) 192, 459, 642, 873, 1053
MslI CAYNNNNRTG 2 cut(s) 660, 1335
Msp20I TGGCCA 1 cut(s) 895
MspI CCGG 3 cut(s) 14, 695, 1387
MspR9I CCNGG 3 cut(s) 14, 696, 1272
MunI CAATTG 1 cut(s) 176
Mva1269I GAATGC 2 cut(s) 401, 827
MvaI CCWGG 1 cut(s) 1272
MvnI CGCG 1 cut(s) 675
NciI CCSGG 2 cut(s) 14, 696
NdeI CATATG 2 cut(s) 1234, 1451
NlaIV GGNNCC 3 cut(s) 651, 1021, 1132
NmuCI GTSAC 5 cut(s) 16, 96, 1042, 1201, 1445
NsiI ATGCAT 1 cut(s) 1409
NspI RCATGY 3 cut(s) 610, 1411, 1618
PaeR7I CTCGAG 1 cut(s) 250
PagI TCATGA 1 cut(s) 1155
PctI GAATGC 2 cut(s) 401, 827
PdmI GAANNNNTTC 1 cut(s) 913
PfeI GAWTC 2 cut(s) 290, 758
PflMI CCANNNNNTGG 1 cut(s) 387
PfoI TCCNGGA 1 cut(s) 1270
PinAI ACCGGT 1 cut(s) 1386
PleI GAGTC 2 cut(s) 499, 1520
PpsI GAGTC 2 cut(s) 499, 1520
Psp6I CCWGG 1 cut(s) 1270
PspEI GGTNACC 1 cut(s) 96
PspGI CCWGG 1 cut(s) 1270
PspN4I GGNNCC 3 cut(s) 651, 1021, 1132
PspPI GGNCC 4 cut(s) 560, 1020, 1130, 1389
PsuI RGATCY 4 cut(s) 274, 524, 632, 1399
RsaI GTAC 5 cut(s) 379, 651, 1151, 1462, 1498
RsaNI GTAC 5 cut(s) 378, 650, 1150, 1461, 1497
RseI CAYNNNNRTG 2 cut(s) 660, 1335
SaqAI TTAA 5 cut(s) 192, 459, 642, 873, 1053
Sau96I GGNCC 4 cut(s) 560, 1020, 1130, 1389
ScaI AGTACT 1 cut(s) 1498
SchI GAGTC 2 cut(s) 500, 1520
ScrFI CCNGG 3 cut(s) 14, 696, 1272
SduI GDGCHC 2 cut(s) 973, 1522
SfaNI GCATC 2 cut(s) 1034, 1370
SfcI CTRYAG 3 cut(s) 42, 845, 1500
Sfr274I CTCGAG 1 cut(s) 250
SinI GGWCC 4 cut(s) 560, 1020, 1130, 1389
SlaI CTCGAG 1 cut(s) 250
SmiMI CAYNNNNRTG 2 cut(s) 660, 1335
SmlI CTYRAG 1 cut(s) 250
SmoI CTYRAG 1 cut(s) 250
SpeI ACTAGT 1 cut(s) 1046
Sse9I AATT 9 cut(s) 157, 176, 460, 539, 585, 621, 838, 1074, 1119
SsiI CCGC 5 cut(s) 10, 270, 1023, 1430, 1482
SspI AATATT 1 cut(s) 80
SspMI CTAG 2 cut(s) 1047, 1654
StyD4I CCNGG 3 cut(s) 12, 694, 1270
StyI CCWWGG 2 cut(s) 381, 1206
TaaI ACNGT 7 cut(s) 22, 331, 362, 557, 718, 878, 992
TaqI TCGA 2 cut(s) 251, 448
TaqII GACCGA 1 cut(s) 1030
TasI AATT 9 cut(s) 157, 176, 460, 539, 585, 621, 838, 1074, 1119
TatI WGTACW 2 cut(s) 1149, 1496
TfiI GAWTC 2 cut(s) 290, 758
Tru1I TTAA 5 cut(s) 192, 459, 642, 873, 1053
Tru9I TTAA 5 cut(s) 192, 459, 642, 873, 1053
TscAI CASTG 9 cut(s) 27, 169, 300, 367, 586, 808, 1015, 1375, 1544
TseFI GTSAC 5 cut(s) 16, 96, 1042, 1201, 1445
Tsp45I GTSAC 5 cut(s) 16, 96, 1042, 1201, 1445
TspDTI ATGAA 7 cut(s) 215, 324, 408, 858, 906, 1144, 1570
TspGWI ACGGA 3 cut(s) 302, 756, 1236
TspRI CASTG 9 cut(s) 27, 169, 300, 367, 586, 808, 1015, 1375, 1544
Van91I CCANNNNNTGG 1 cut(s) 387
VpaK11BI GGWCC 4 cut(s) 560, 1020, 1130, 1389
XagI CCTNNNNNAGG 1 cut(s) 726
XapI RAATTY 3 cut(s) 585, 1074, 1119
XceI RCATGY 3 cut(s) 610, 1411, 1618
XhoI CTCGAG 1 cut(s) 250
XmnI GAANNNNTTC 1 cut(s) 913
XspI CTAG 2 cut(s) 1047, 1654
ZrmI AGTACT 1 cut(s) 1498
Zsp2I ATGCAT 1 cut(s) 1409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.