Rroxscaffold_2G00120880

Belongs to the mitochondrial carrier (TC 2.A.29) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0021333
Physical Location & Seq
Reverse (-)
53330027 .. 53331151
1125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00120880.1

Sequence Viewer

Length: 669 bp
ATGTATAGAATCATCTATGGAGGAATACTAAAGGATGGGGAAGAGGAGTTAGGCTATGAAGAGGAGGATGGGGAAGGAGTGGAGCCCACCACTCATGGTCTATTTAAATTGAATTGTGATGGTCCCTCTAATTCACAGTTTGATAGATTTGGAGTTGGTGTAGTGCTTAGGGATGAACATTGTACTTTGATTGTGGGAGACAACTCTATTGTGTATTATGGTCTATACTTTGGAATGTATGATTCTTTGAAGCTTGTGCTCCTCACCGGAAAGATGCGGGATAGTTTCTTTTTTAGCTTTGCTCTTGGTTGGGTTATCACCAATGGTGTTGGTCTTGCATTTTACCCAATTGACATTGTCCGTAGAAGAATGATGACGACTTCTAGTGAAGCCGTCAAATACAAGCACTCGCCCGATTGCTTCAATCAAATCCTTAAGAATGAGGGTGCAAAGTCTTTCTTCAAGGGTGCCGGTGCAAATATTCTTCGTGCAATTGCTGGTCTTTGGTGTGCTTGCACGTTATTACAAGCTTTAGGTGCTAGTGTTTGGAAGAAGTATAGTTCGGTTGAGCTTAAGCAAAAAATGATCGTTTTTTGTGGGGCTTCGATAAAAGTGAATGACCCTATGAACATTGTTTTCATAACCATCTTGGCTATAAGAGCTGTTTAG

Protein Analysis

222

Amino Acids

24.68

Weight (kDa)

6.82

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 96 - 167 1e-13 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0021333)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0123151
rosa_multiflora Rmu_co8334221.1_g000001 Rmu_sc0005573.1_g000007
rosa_roxburghii Rroxscaffold_2G00120880
rosa_samantha Rh2BG310800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 467
AciI CCGC 1 cut(s) 277
AfaI GTAC 1 cut(s) 184
AflII CTTAAG 2 cut(s) 434, 572
AgsI TTSAA 4 cut(s) 112, 250, 424, 463
AluBI AGCT 5 cut(s) 253, 297, 530, 571, 662
AluI AGCT 5 cut(s) 253, 297, 530, 571, 662
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 1 cut(s) 192
AspS9I GGNCC 1 cut(s) 122
AsuHPI GGTGA 2 cut(s) 256, 310
AvaII GGWCC 1 cut(s) 122
BanI GGYRCC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 261
BccI CCATC 4 cut(s) 29, 62, 113, 653
BceAI ACGGC 1 cut(s) 377
BcoDI GTCTC 1 cut(s) 192
BfaI CTAG 2 cut(s) 384, 540
BfrI CTTAAG 2 cut(s) 434, 572
Bme18I GGWCC 1 cut(s) 122
BmgT120I GGNCC 1 cut(s) 122
BmiI GGNNCC 3 cut(s) 84, 124, 469
BmsI GCATC 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 167
BsaWI WCCGGW 1 cut(s) 266
BsaXI ACNNNNNCTCC 2 cut(s) 144, 174
Bse118I RCCGGY 1 cut(s) 470
BseGI GGATG 3 cut(s) 40, 73, 178
BseRI GAGGAG 3 cut(s) 59, 77, 251
BshNI GGYRCC 1 cut(s) 467
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 2 cut(s) 267, 471
BslFI GGGAC 1 cut(s) 108
BsmAI GTCTC 1 cut(s) 192
BsmFI GGGAC 1 cut(s) 108
Bsp1286I GDGCHC 2 cut(s) 87, 261
Bsp143I GATC 1 cut(s) 585
BspACI CCGC 1 cut(s) 277
BspLI GGNNCC 3 cut(s) 84, 124, 469
BspT107I GGYRCC 1 cut(s) 467
BspTI CTTAAG 2 cut(s) 434, 572
BsrFI RCCGGY 1 cut(s) 470
BssAI RCCGGY 1 cut(s) 470
BssMI GATC 1 cut(s) 585
Bst4CI ACNGT 1 cut(s) 138
Bst6I CTCTTC 2 cut(s) 36, 54
BstAFI CTTAAG 2 cut(s) 434, 572
BstC8I GCNNGC 1 cut(s) 514
BstDEI CTNAG 1 cut(s) 167
BstF5I GGATG 3 cut(s) 40, 73, 178
BstKTI GATC 1 cut(s) 588
BstMAI GTCTC 1 cut(s) 192
BstMBI GATC 1 cut(s) 585
BstMWI GCNNNNNNNGC 2 cut(s) 536, 659
BtsCI GGATG 3 cut(s) 40, 73, 178
Cac8I GCNNGC 1 cut(s) 514
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 1 cut(s) 122
Csp6I GTAC 1 cut(s) 183
CviAII CATG 1 cut(s) 95
CviQI GTAC 1 cut(s) 183
DdeI CTNAG 1 cut(s) 167
DpnI GATC 1 cut(s) 587
DpnII GATC 1 cut(s) 585
DraI TTTAAA 1 cut(s) 106
Eam1104I CTCTTC 2 cut(s) 36, 54
EarI CTCTTC 2 cut(s) 36, 54
Eco24I GRGCYC 1 cut(s) 87
Eco47I GGWCC 1 cut(s) 122
EcoT38I GRGCYC 1 cut(s) 87
FaeI CATG 1 cut(s) 98
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FaqI GGGAC 1 cut(s) 108
FatI CATG 1 cut(s) 94
FauI CCCGC 1 cut(s) 270
FokI GGATG 3 cut(s) 47, 80, 185
FriOI GRGCYC 1 cut(s) 87
FspBI CTAG 2 cut(s) 384, 540
HapII CCGG 2 cut(s) 267, 471
Hin1II CATG 1 cut(s) 98
HindIII AAGCTT 2 cut(s) 251, 528
HinfI GANTC 2 cut(s) 9, 242
HpaII CCGG 2 cut(s) 267, 471
HphI GGTGA 2 cut(s) 256, 310
HpyAV CCTTC 1 cut(s) 68
HpyCH4III ACNGT 1 cut(s) 138
HpyCH4IV ACGT 1 cut(s) 518
HpyCH4V TGCA 5 cut(s) 338, 449, 476, 491, 516
HpyF10VI GCNNNNNNNGC 2 cut(s) 536, 659
HpyF3I CTNAG 1 cut(s) 167
HpySE526I ACGT 1 cut(s) 518
Hsp92II CATG 1 cut(s) 98
Kzo9I GATC 1 cut(s) 585
LmnI GCTCC 2 cut(s) 82, 264
LpnPI CCDG 3 cut(s) 280, 483, 484
LweI GCATC 1 cut(s) 264
MaeI CTAG 2 cut(s) 384, 540
MaeII ACGT 1 cut(s) 518
MalI GATC 1 cut(s) 587
MboI GATC 1 cut(s) 585
MboII GAAGA 6 cut(s) 53, 71, 378, 451, 476, 562
MfeI CAATTG 2 cut(s) 348, 492
MhlI GDGCHC 2 cut(s) 87, 261
MluCI AATT 5 cut(s) 107, 112, 130, 348, 492
MnlI CCTC 7 cut(s) 14, 37, 55, 58, 136, 272, 436
MseI TTAA 3 cut(s) 105, 435, 573
MspCI CTTAAG 2 cut(s) 434, 572
MspI CCGG 2 cut(s) 267, 471
MunI CAATTG 2 cut(s) 348, 492
MwoI GCNNNNNNNGC 2 cut(s) 536, 659
NdeII GATC 1 cut(s) 585
NlaIII CATG 1 cut(s) 98
NlaIV GGNNCC 3 cut(s) 84, 124, 469
PfeI GAWTC 2 cut(s) 9, 242
PflFI GACNNNGTC 1 cut(s) 356
PspN4I GGNNCC 3 cut(s) 84, 124, 469
PspPI GGNCC 1 cut(s) 122
PsyI GACNNNGTC 1 cut(s) 356
RsaI GTAC 1 cut(s) 184
RsaNI GTAC 1 cut(s) 183
SaqAI TTAA 3 cut(s) 105, 435, 573
Sau3AI GATC 1 cut(s) 585
Sau96I GGNCC 1 cut(s) 122
SduI GDGCHC 2 cut(s) 87, 261
SetI ASST 7 cut(s) 255, 299, 521, 532, 538, 573, 664
SfaNI GCATC 1 cut(s) 264
SinI GGWCC 1 cut(s) 122
SmiI ATTTAAAT 1 cut(s) 106
SmlI CTYRAG 2 cut(s) 434, 572
SmoI CTYRAG 2 cut(s) 434, 572
Sse9I AATT 5 cut(s) 107, 112, 130, 348, 492
SsiI CCGC 1 cut(s) 277
SspI AATATT 1 cut(s) 481
SspMI CTAG 2 cut(s) 384, 540
SwaI ATTTAAAT 1 cut(s) 106
TaaI ACNGT 1 cut(s) 138
TaiI ACGT 1 cut(s) 521
TaqI TCGA 1 cut(s) 605
TasI AATT 5 cut(s) 107, 112, 130, 348, 492
TatI WGTACW 1 cut(s) 182
TfiI GAWTC 2 cut(s) 9, 242
Tru1I TTAA 3 cut(s) 105, 435, 573
Tru9I TTAA 3 cut(s) 105, 435, 573
TspDTI ATGAA 4 cut(s) 72, 189, 628, 641
TspGWI ACGGA 1 cut(s) 350
Tth111I GACNNNGTC 1 cut(s) 356
Vha464I CTTAAG 2 cut(s) 434, 572
VpaK11BI GGWCC 1 cut(s) 122
XspI CTAG 2 cut(s) 384, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.