Rroxscaffold_2G00132320

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0023116
Physical Location & Seq
Forward (+)
69354092 .. 69354559
468 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_2G00132320.1

Sequence Viewer

Length: 468 bp
ATGTTTCTAGGGCGCTCTCTGCGACCCCCAGCCTTGTTAGGAAGTAACGCCCTTTCTCATGCTGGGACATACAATAGAAGAAACTCGGAAGCTGTCCCTTCAAGTCTTAGCAAATCCAAGTTGAGATACACGGTTCCAAGTATGAATAGTTCCACAGGACAACAAAAGTCTAGTAGAGACCGGTACAGGATGAACTTTGATGGTTCAAGCAATCAATCATTAGATCCAGATGATTGGAGATCTCATTCCATGTACAGAGCTCCTGCTATGGATTCATCAAGTGAGAGTTCGTCGATTTTCAGCTCCTCGGATGCAAGTTCTTGTAGCAGCGTGAGCACCACGGACTCCTCTAGCAATGAAGATTTCTCTGATTACATATTTGGTCAAGTAGGAACAAATCCATACAGCCAATTCAGGCGTTCATCAGACGTGGTTACACCTTCCTTCTGTTTCCAAACTTTGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UCH zf-MYND

Protein Analysis

155

Amino Acids

17.03

Weight (kDa)

7.79

Isoelectric Point (pI)

66.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023116)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0005791.1_g000001
rosa_roxburghii Rroxscaffold_2G00132320
rosa_rugosa Rorug02G0174200
rosa_samantha Rh2BG238700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 218
AfaI GTAC 2 cut(s) 185, 254
AgeI ACCGGT 1 cut(s) 180
AgsI TTSAA 2 cut(s) 102, 207
AjiI CACGTC 1 cut(s) 430
AluBI AGCT 3 cut(s) 92, 260, 303
AluI AGCT 3 cut(s) 92, 260, 303
Alw21I GWGCWC 2 cut(s) 262, 338
Alw26I GTCTC 1 cut(s) 171
AlwI GGATC 1 cut(s) 218
ApeKI GCWGC 1 cut(s) 327
AsiGI ACCGGT 1 cut(s) 180
AspLEI GCGC 1 cut(s) 15
BanII GRGCYC 1 cut(s) 262
Bbv12I GWGCWC 2 cut(s) 262, 338
BbvI GCAGC 1 cut(s) 339
BccI CCATC 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 3 cut(s) 8, 171, 351
BfoI RGCGCY 1 cut(s) 16
BglII AGATCT 1 cut(s) 239
BisI GCNGC 1 cut(s) 328
BlsI GCNGC 1 cut(s) 329
BmgBI CACGTC 1 cut(s) 430
BmiI GGNNCC 1 cut(s) 135
BmsI GCATC 1 cut(s) 301
BsaI GGTCTC 1 cut(s) 171
BsaJI CCNNGG 2 cut(s) 306, 339
BsaWI WCCGGW 1 cut(s) 180
Bse118I RCCGGY 1 cut(s) 180
Bse3DI GCAATG 1 cut(s) 361
BseDI CCNNGG 2 cut(s) 306, 339
BseGI GGATG 2 cut(s) 195, 316
BseMI GCAATG 1 cut(s) 361
BseRI GAGGAG 2 cut(s) 295, 337
BseXI GCAGC 1 cut(s) 339
BseYI CCCAGC 2 cut(s) 28, 62
BshTI ACCGGT 1 cut(s) 180
BsiHKAI GWGCWC 2 cut(s) 262, 338
BsiSI CCGG 1 cut(s) 181
BslFI GGGAC 2 cut(s) 79, 80
BsmAI GTCTC 1 cut(s) 171
BsmFI GGGAC 2 cut(s) 79, 80
Bso31I GGTCTC 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 262, 338
Bsp1407I TGTACA 1 cut(s) 252
Bsp143I GATC 2 cut(s) 223, 239
BspLI GGNNCC 1 cut(s) 135
BspPI GGATC 1 cut(s) 218
BspTNI GGTCTC 1 cut(s) 171
BsrDI GCAATG 1 cut(s) 361
BsrFI RCCGGY 1 cut(s) 180
BsrGI TGTACA 1 cut(s) 252
BssAI RCCGGY 1 cut(s) 180
BssECI CCNNGG 2 cut(s) 306, 339
BssMI GATC 2 cut(s) 223, 239
Bst4CI ACNGT 1 cut(s) 133
BstAUI TGTACA 1 cut(s) 252
BstDEI CTNAG 1 cut(s) 107
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 2 cut(s) 195, 316
BstH2I RGCGCY 1 cut(s) 16
BstHHI GCGC 1 cut(s) 15
BstKTI GATC 2 cut(s) 226, 242
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 2 cut(s) 223, 239
BstMWI GCNNNNNNNGC 2 cut(s) 19, 333
BstV1I GCAGC 1 cut(s) 339
BstX2I RGATCY 2 cut(s) 223, 239
BstXI CCANNNNNNTGG 1 cut(s) 234
BstYI RGATCY 2 cut(s) 223, 239
BtgI CCRYGG 1 cut(s) 339
BtrI CACGTC 1 cut(s) 430
BtsCI GGATG 2 cut(s) 195, 316
CfoI GCGC 1 cut(s) 15
Cfr10I RCCGGY 1 cut(s) 180
Csp6I GTAC 2 cut(s) 184, 253
CspAI ACCGGT 1 cut(s) 180
CviAII CATG 2 cut(s) 59, 250
CviJI RGCY 5 cut(s) 32, 92, 260, 303, 408
CviKI_1 RGCY 5 cut(s) 32, 92, 260, 303, 408
CviQI GTAC 2 cut(s) 184, 253
DdeI CTNAG 1 cut(s) 107
DpnI GATC 2 cut(s) 225, 241
DpnII GATC 2 cut(s) 223, 239
Ecl136II GAGCTC 1 cut(s) 260
Eco24I GRGCYC 1 cut(s) 262
Eco31I GGTCTC 1 cut(s) 171
Eco53kI GAGCTC 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 260
EcoT38I GRGCYC 1 cut(s) 262
FaeI CATG 2 cut(s) 62, 253
FaiI YATR 7 cut(s) 60, 70, 143, 251, 269, 377, 403
FaqI GGGAC 2 cut(s) 79, 80
FatI CATG 2 cut(s) 58, 249
Fnu4HI GCNGC 1 cut(s) 328
FokI GGATG 2 cut(s) 202, 323
FriOI GRGCYC 1 cut(s) 262
Fsp4HI GCNGC 1 cut(s) 328
FspBI CTAG 3 cut(s) 8, 171, 351
GlaI GCGC 1 cut(s) 14
GluI GCNGC 1 cut(s) 328
GsaI CCCAGC 2 cut(s) 32, 66
HaeII RGCGCY 1 cut(s) 16
HapII CCGG 1 cut(s) 181
HhaI GCGC 1 cut(s) 15
Hin1II CATG 2 cut(s) 62, 253
Hin6I GCGC 1 cut(s) 13
HinP1I GCGC 1 cut(s) 13
HinfI GANTC 2 cut(s) 272, 344
HpaII CCGG 1 cut(s) 181
Hpy188I TCNGA 4 cut(s) 88, 310, 370, 427
Hpy188III TCNNGA 1 cut(s) 227
Hpy99I CGWCG 1 cut(s) 295
HpyAV CCTTC 3 cut(s) 108, 450, 454
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4IV ACGT 1 cut(s) 429
HpyCH4V TGCA 1 cut(s) 314
HpyF10VI GCNNNNNNNGC 2 cut(s) 19, 333
HpyF3I CTNAG 1 cut(s) 107
HpySE526I ACGT 1 cut(s) 429
Hsp92II CATG 2 cut(s) 62, 253
HspAI GCGC 1 cut(s) 13
Kzo9I GATC 2 cut(s) 223, 239
LmnI GCTCC 2 cut(s) 265, 308
LpnPI CCDG 8 cut(s) 42, 48, 141, 172, 194, 240, 276, 400
Lsp1109I GCAGC 1 cut(s) 339
LweI GCATC 1 cut(s) 301
MaeI CTAG 3 cut(s) 8, 171, 351
MaeII ACGT 1 cut(s) 429
MaeIII GTNAC 2 cut(s) 44, 433
MalI GATC 2 cut(s) 225, 241
MboI GATC 2 cut(s) 223, 239
MboII GAAGA 2 cut(s) 90, 371
MflI RGATCY 2 cut(s) 223, 239
MhlI GDGCHC 2 cut(s) 262, 338
MluCI AATT 1 cut(s) 410
MlyI GAGTC 1 cut(s) 338
MnlI CCTC 2 cut(s) 316, 358
MspI CCGG 1 cut(s) 181
MwoI GCNNNNNNNGC 2 cut(s) 19, 333
NdeII GATC 2 cut(s) 223, 239
NlaIII CATG 2 cut(s) 62, 253
NlaIV GGNNCC 1 cut(s) 135
PfeI GAWTC 1 cut(s) 272
PinAI ACCGGT 1 cut(s) 180
PkrI GCNGC 1 cut(s) 329
PleI GAGTC 1 cut(s) 338
PpsI GAGTC 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 262
PspFI CCCAGC 2 cut(s) 28, 62
PspN4I GGNNCC 1 cut(s) 135
PsrI GAACNNNNNNTAC 2 cut(s) 133, 165
PsuI RGATCY 2 cut(s) 223, 239
RsaI GTAC 2 cut(s) 185, 254
RsaNI GTAC 2 cut(s) 184, 253
SacI GAGCTC 1 cut(s) 262
SatI GCNGC 1 cut(s) 328
Sau3AI GATC 2 cut(s) 223, 239
SchI GAGTC 1 cut(s) 338
SduI GDGCHC 2 cut(s) 262, 338
SetI ASST 5 cut(s) 94, 262, 305, 432, 442
SfaNI GCATC 1 cut(s) 301
Sse9I AATT 1 cut(s) 410
SspMI CTAG 3 cut(s) 8, 171, 351
SstI GAGCTC 1 cut(s) 262
TaaI ACNGT 1 cut(s) 133
TaiI ACGT 1 cut(s) 432
TaqI TCGA 1 cut(s) 293
TasI AATT 1 cut(s) 410
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 1 cut(s) 272
TseI GCWGC 1 cut(s) 327
TspDTI ATGAA 5 cut(s) 158, 206, 264, 372, 411
TspGWI ACGGA 1 cut(s) 356
XspI CTAG 3 cut(s) 8, 171, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.