Rroxscaffold_2G00134250

Auxin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0024831
Physical Location & Seq
Reverse (-)
71743129 .. 71743737
609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00134250.1

Sequence Viewer

Length: 468 bp
ATGGCCTTGGTATTTCGCTGGCTCGTACGGATTTGGCGGTTGGTAGAGTTGTTCCGATGCACACTCACCACGGAGCTTCAGAGGTCATACTTGTTGCGGAAGGCAAAGTTAATAGCTGGGTTGATTGCCTCGGATAACAAAGCTTATGTAAAAAATCTGAAGAAAGGTGATATTATGGTTTTACCTCAAGGTTTGCTTCACTTCCAAGTAAATGCAGGTGATACTCCAACCCTTGTGTTTGCTATCTTCAGTAGTGACGACCCAAGTGTGCAGGTTTTGGAGACTGAACTGTTTCAGAATCATTTACGTACTGAATTGATAGCACAGACTACTCTCCTTCACACTGCTGAGATTAAGAAACTTAAGGGTCTTCTTGGCGGCACAAAGCAGCTCAGGCACTATGGAACTGGAAACCTTTCTCTGCTAACAGAAACTGAATGGCAAAAGGAAACTAAACTAGAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0048046 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

155

Amino Acids

17.63

Weight (kDa)

9.44

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 38 - 115 5.9e-14 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024831)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0109421
rosa_laevigata RLG00000017737
rosa_roxburghii Rroxscaffold_2G00134250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 206
Acc36I ACCTGC 2 cut(s) 206, 262
AciI CCGC 3 cut(s) 37, 97, 378
AcuI CTGAAG 3 cut(s) 62, 179, 232
AfaI GTAC 2 cut(s) 27, 310
AflII CTTAAG 1 cut(s) 362
AluBI AGCT 4 cut(s) 76, 116, 143, 391
AluI AGCT 4 cut(s) 76, 116, 143, 391
Alw26I GTCTC 1 cut(s) 275
AlwNI CAGNNNCTG 1 cut(s) 434
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 388
Asp700I GAANNNNTTC 2 cut(s) 291, 415
AsuHPI GGTGA 3 cut(s) 58, 179, 230
BbsI GAAGAC 1 cut(s) 362
BbvI GCAGC 1 cut(s) 400
BcoDI GTCTC 1 cut(s) 275
BfaI CTAG 1 cut(s) 458
BfrI CTTAAG 1 cut(s) 362
BfuAI ACCTGC 2 cut(s) 206, 262
BisI GCNGC 2 cut(s) 379, 389
BlsI GCNGC 2 cut(s) 380, 390
BmsI GCATC 1 cut(s) 47
BpiI GAAGAC 1 cut(s) 362
Bpu10I CCTNAGC 1 cut(s) 392
BpuEI CTTGAG 1 cut(s) 171
BsaAI YACGTR 1 cut(s) 308
BsaJI CCNNGG 3 cut(s) 6, 69, 129
Bse1I ACTGG 1 cut(s) 412
BseDI CCNNGG 3 cut(s) 6, 69, 129
BseMII CTCAG 2 cut(s) 339, 406
BseNI ACTGG 1 cut(s) 412
BseXI GCAGC 1 cut(s) 400
BseYI CCCAGC 1 cut(s) 116
BsgI GTGCAG 1 cut(s) 290
BshFI GGCC 1 cut(s) 5
BsiWI CGTACG 1 cut(s) 25
BsmAI GTCTC 1 cut(s) 275
BsnI GGCC 1 cut(s) 5
BspACI CCGC 3 cut(s) 37, 97, 378
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 340, 405
BspMI ACCTGC 2 cut(s) 206, 262
BspTI CTTAAG 1 cut(s) 362
BsrI ACTGG 1 cut(s) 412
BssECI CCNNGG 3 cut(s) 6, 69, 129
BssT1I CCWWGG 1 cut(s) 6
Bst4CI ACNGT 1 cut(s) 291
BstAFI CTTAAG 1 cut(s) 362
BstBAI YACGTR 1 cut(s) 308
BstC8I GCNNGC 1 cut(s) 20
BstDEI CTNAG 2 cut(s) 348, 392
BstDSI CCRYGG 1 cut(s) 69
BstMAI GTCTC 1 cut(s) 275
BstMWI GCNNNNNNNGC 1 cut(s) 394
BstSNI TACGTA 1 cut(s) 308
BstV1I GCAGC 1 cut(s) 400
BstV2I GAAGAC 1 cut(s) 362
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 69
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 1 cut(s) 342
BveI ACCTGC 2 cut(s) 206, 262
Cac8I GCNNGC 1 cut(s) 20
CaiI CAGNNNCTG 1 cut(s) 434
Csp6I GTAC 2 cut(s) 26, 309
CviJI RGCY 7 cut(s) 5, 22, 76, 116, 143, 391, 464
CviKI_1 RGCY 7 cut(s) 5, 22, 76, 116, 143, 391, 464
CviQI GTAC 2 cut(s) 26, 309
DdeI CTNAG 2 cut(s) 348, 392
Eco105I TACGTA 1 cut(s) 308
Eco130I CCWWGG 1 cut(s) 6
Eco57I CTGAAG 3 cut(s) 62, 179, 232
EcoT14I CCWWGG 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 6
FaiI YATR 4 cut(s) 88, 147, 176, 402
FalI AAGNNNNNCTT 2 cut(s) 180, 212
Fnu4HI GCNGC 2 cut(s) 379, 389
Fsp4HI GCNGC 2 cut(s) 379, 389
FspBI CTAG 1 cut(s) 458
GluI GCNGC 2 cut(s) 379, 389
GsaI CCCAGC 1 cut(s) 120
HaeIII GGCC 1 cut(s) 5
HindIII AAGCTT 1 cut(s) 141
HinfI GANTC 1 cut(s) 298
HphI GGTGA 3 cut(s) 58, 179, 230
Hpy188I TCNGA 5 cut(s) 56, 81, 133, 159, 297
HpyAV CCTTC 2 cut(s) 94, 347
HpyCH4III ACNGT 1 cut(s) 291
HpyCH4IV ACGT 1 cut(s) 307
HpyCH4V TGCA 3 cut(s) 60, 215, 271
HpyF10VI GCNNNNNNNGC 1 cut(s) 394
HpyF3I CTNAG 2 cut(s) 348, 392
HpySE526I ACGT 1 cut(s) 307
LmnI GCTCC 1 cut(s) 73
LpnPI CCDG 6 cut(s) 4, 102, 201, 257, 379, 393
Lsp1109I GCAGC 1 cut(s) 400
LweI GCATC 1 cut(s) 47
MaeI CTAG 1 cut(s) 458
MaeII ACGT 1 cut(s) 307
MaeIII GTNAC 1 cut(s) 254
MboII GAAGA 3 cut(s) 172, 238, 362
MluCI AATT 1 cut(s) 314
MmeI TCCRAC 1 cut(s) 251
MnlI CCTC 4 cut(s) 75, 139, 195, 454
MroXI GAANNNNTTC 2 cut(s) 291, 415
MseI TTAA 3 cut(s) 110, 354, 363
MspCI CTTAAG 1 cut(s) 362
MwoI GCNNNNNNNGC 1 cut(s) 394
NmuCI GTSAC 1 cut(s) 254
PaqCI CACCTGC 1 cut(s) 206
PdmI GAANNNNTTC 2 cut(s) 291, 415
PfeI GAWTC 1 cut(s) 298
Pfl23II CGTACG 1 cut(s) 25
PkrI GCNGC 2 cut(s) 380, 390
Ppu21I YACGTR 1 cut(s) 308
PspFI CCCAGC 1 cut(s) 116
PspLI CGTACG 1 cut(s) 25
PstNI CAGNNNCTG 1 cut(s) 434
RsaI GTAC 2 cut(s) 27, 310
RsaNI GTAC 2 cut(s) 26, 309
SaqAI TTAA 3 cut(s) 110, 354, 363
SatI GCNGC 2 cut(s) 379, 389
SfaNI GCATC 1 cut(s) 47
SmlI CTYRAG 2 cut(s) 186, 362
SmoI CTYRAG 2 cut(s) 186, 362
SnaBI TACGTA 1 cut(s) 308
Sse9I AATT 1 cut(s) 314
SsiI CCGC 3 cut(s) 37, 97, 378
SspMI CTAG 1 cut(s) 458
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 291
TaiI ACGT 1 cut(s) 310
TasI AATT 1 cut(s) 314
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 1 cut(s) 298
Tru1I TTAA 3 cut(s) 110, 354, 363
Tru9I TTAA 3 cut(s) 110, 354, 363
TscAI CASTG 1 cut(s) 349
TseFI GTSAC 1 cut(s) 254
TseI GCWGC 1 cut(s) 388
Tsp45I GTSAC 1 cut(s) 254
TspGWI ACGGA 2 cut(s) 43, 86
TspRI CASTG 1 cut(s) 349
Vha464I CTTAAG 1 cut(s) 362
XmnI GAANNNNTTC 2 cut(s) 291, 415
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.