Rroxscaffold_3G00226850

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0025182
Physical Location & Seq
Reverse (-)
10648146 .. 10650660
2515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00226850.1

Sequence Viewer

Length: 558 bp
ATGATGATGTGCGCATGGTTGGAATTTGGGGTATGGGTGGAAGTGGGAAAACATCAGTTGCAAAAAGCTGTCTATAACAAATTTTATCATACCTTTGAAAGTAAAAGTTTCCTTGGAAATGTTAGGGAAACTGCAAAGGACTCAAATGGTATGATAACTTTGCAAGAGAGACTTGTTTCTGATGTCTTAAACCCAACCAAGATTGAGGTAGGTAATGTTCCTAGAGGGATCAATGTGAATAAACAAAGGCTTGGAAGCAAAAAGGTACTTGACATTGTTGATGATGTAGATGATGAAGACCAACTAACTGCATTAGCTATAAGGCATGATTCATTTGGTTGGGGAAGTAGGATTATTAGAACGACAAGAGAGCTACATTTGCTAGAGCTACTTAAAGTGGATACAGTACATCTGACTCGAGAAATGAAGGAAGAAGAAGCTCTTCAGCTCTTTAGTTGGCGTGCGTTTCGAAATCCTTGTCCTCATTTAGGATTTTTGGAACTCACAAGGAGAGTGGTTACATACTGTGGAGGTTTACCACTGGCTCTTGAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

185

Amino Acids

21.27

Weight (kDa)

6.6

Isoelectric Point (pI)

30.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 15 - 158 1.2e-07 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0025182)

Species Orthologous Gene IDs
rosa_laevigata RLG00000001234
rosa_roxburghii Rroxscaffold_3G00226850
rosa_rugosa Rorug07G0284200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 13
AclWI GGATC 1 cut(s) 236
AcsI RAATTY 2 cut(s) 23, 80
AcuI CTGAAG 1 cut(s) 428
AfaI GTAC 2 cut(s) 267, 408
AfiI CCNNNNNNNGG 1 cut(s) 488
AgsI TTSAA 2 cut(s) 98, 551
AluBI AGCT 6 cut(s) 68, 317, 373, 388, 440, 448
AluI AGCT 6 cut(s) 68, 317, 373, 388, 440, 448
Alw26I GTCTC 1 cut(s) 163
AlwI GGATC 1 cut(s) 236
Ama87I CYCGRG 1 cut(s) 417
ApoI RAATTY 2 cut(s) 23, 80
ArsI GACNNNNNNTTYG 2 cut(s) 463, 495
Asp700I GAANNNNTTC 1 cut(s) 441
AspLEI GCGC 1 cut(s) 14
AsuII TTCGAA 1 cut(s) 469
AvaI CYCGRG 1 cut(s) 417
BbsI GAAGAC 1 cut(s) 303
BciVI GTATCC 1 cut(s) 394
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 2 cut(s) 222, 383
BfuI GTATCC 1 cut(s) 394
BmeT110I CYCGRG 1 cut(s) 417
BpiI GAAGAC 1 cut(s) 303
Bpu14I TTCGAA 1 cut(s) 469
BsaJI CCNNGG 1 cut(s) 112
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse1I ACTGG 1 cut(s) 546
BseDI CCNNGG 1 cut(s) 112
BseLI CCNNNNNNNGG 1 cut(s) 488
BseNI ACTGG 1 cut(s) 546
BsiHKCI CYCGRG 1 cut(s) 417
BslI CCNNNNNNNGG 1 cut(s) 488
BsmAI GTCTC 1 cut(s) 163
BsoBI CYCGRG 1 cut(s) 417
Bsp119I TTCGAA 1 cut(s) 469
Bsp143I GATC 1 cut(s) 228
BspPI GGATC 1 cut(s) 236
BspQI GCTCTTC 1 cut(s) 447
BspT104I TTCGAA 1 cut(s) 469
BsrI ACTGG 1 cut(s) 546
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 1 cut(s) 228
BssT1I CCWWGG 1 cut(s) 112
Bst4CI ACNGT 2 cut(s) 406, 527
Bst6I CTCTTC 1 cut(s) 447
BstBI TTCGAA 1 cut(s) 469
BstC8I GCNNGC 1 cut(s) 462
BstENI CCTNNNNNAGG 1 cut(s) 486
BstHHI GCGC 1 cut(s) 14
BstKTI GATC 1 cut(s) 231
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 1 cut(s) 228
BstMWI GCNNNNNNNGC 1 cut(s) 379
BstV2I GAAGAC 1 cut(s) 303
BsuI GTATCC 1 cut(s) 394
BtsIMutI CAGTG 1 cut(s) 539
Cac8I GCNNGC 1 cut(s) 462
CfoI GCGC 1 cut(s) 14
Csp6I GTAC 2 cut(s) 266, 407
CspCI CAANNNNNGTGG 2 cut(s) 495, 530
CviAII CATG 2 cut(s) 15, 326
CviJI RGCY 8 cut(s) 68, 250, 317, 373, 388, 440, 448, 545
CviKI_1 RGCY 8 cut(s) 68, 250, 317, 373, 388, 440, 448, 545
CviQI GTAC 2 cut(s) 266, 407
DpnI GATC 1 cut(s) 230
DpnII GATC 1 cut(s) 228
Eam1104I CTCTTC 1 cut(s) 447
EarI CTCTTC 1 cut(s) 447
Eco130I CCWWGG 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 428
Eco88I CYCGRG 1 cut(s) 417
EcoNI CCTNNNNNAGG 1 cut(s) 486
EcoT14I CCWWGG 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 112
FaeI CATG 2 cut(s) 18, 329
FaiI YATR 8 cut(s) 16, 34, 75, 90, 152, 320, 327, 523
FalI AAGNNNNNCTT 4 cut(s) 156, 188, 426, 458
FatI CATG 2 cut(s) 14, 325
FspAI RTGCGCAY 1 cut(s) 13
FspBI CTAG 2 cut(s) 222, 383
FspI TGCGCA 1 cut(s) 13
GlaI GCGC 1 cut(s) 13
HhaI GCGC 1 cut(s) 14
Hin1II CATG 2 cut(s) 18, 329
Hin6I GCGC 1 cut(s) 12
HinP1I GCGC 1 cut(s) 12
HinfI GANTC 3 cut(s) 140, 329, 415
Hpy166II GTNNAC 1 cut(s) 536
Hpy188I TCNGA 2 cut(s) 181, 414
Hpy188III TCNNGA 2 cut(s) 419, 548
Hpy8I GTNNAC 1 cut(s) 536
HpyAV CCTTC 1 cut(s) 421
HpyCH4III ACNGT 2 cut(s) 406, 527
HpyCH4V TGCA 4 cut(s) 61, 134, 163, 311
HpyF10VI GCNNNNNNNGC 1 cut(s) 379
Hsp92II CATG 2 cut(s) 18, 329
HspAI GCGC 1 cut(s) 12
Kzo9I GATC 1 cut(s) 228
LguI GCTCTTC 1 cut(s) 447
LpnPI CCDG 1 cut(s) 527
MaeI CTAG 2 cut(s) 222, 383
MaeIII GTNAC 1 cut(s) 517
MalI GATC 1 cut(s) 230
MboI GATC 1 cut(s) 228
MboII GAAGA 4 cut(s) 308, 434, 443, 446
MluCI AATT 2 cut(s) 23, 80
MlyI GAGTC 2 cut(s) 134, 409
MnlI CCTC 4 cut(s) 199, 218, 492, 524
MroXI GAANNNNTTC 1 cut(s) 441
MseI TTAA 3 cut(s) 188, 393, 556
MwoI GCNNNNNNNGC 1 cut(s) 379
NdeII GATC 1 cut(s) 228
NlaIII CATG 2 cut(s) 18, 329
NsbI TGCGCA 1 cut(s) 13
NspV TTCGAA 1 cut(s) 469
PaeR7I CTCGAG 1 cut(s) 417
PciSI GCTCTTC 1 cut(s) 447
PdmI GAANNNNTTC 1 cut(s) 441
PfeI GAWTC 1 cut(s) 329
PleI GAGTC 2 cut(s) 134, 409
PpsI GAGTC 2 cut(s) 134, 409
PsrI GAACNNNNNNTAC 2 cut(s) 201, 233
RsaI GTAC 2 cut(s) 267, 408
RsaNI GTAC 2 cut(s) 266, 407
SapI GCTCTTC 1 cut(s) 447
SaqAI TTAA 3 cut(s) 188, 393, 556
Sau3AI GATC 1 cut(s) 228
SchI GAGTC 2 cut(s) 134, 409
Sfr274I CTCGAG 1 cut(s) 417
SfuI TTCGAA 1 cut(s) 469
SlaI CTCGAG 1 cut(s) 417
SmlI CTYRAG 1 cut(s) 417
SmoI CTYRAG 1 cut(s) 417
Sse9I AATT 2 cut(s) 23, 80
SspMI CTAG 2 cut(s) 222, 383
StyI CCWWGG 1 cut(s) 112
TaaI ACNGT 2 cut(s) 406, 527
TaqI TCGA 2 cut(s) 418, 469
TasI AATT 2 cut(s) 23, 80
TatI WGTACW 1 cut(s) 406
TfiI GAWTC 1 cut(s) 329
Tru1I TTAA 3 cut(s) 188, 393, 556
Tru9I TTAA 3 cut(s) 188, 393, 556
TscAI CASTG 1 cut(s) 546
TspDTI ATGAA 3 cut(s) 309, 321, 440
TspRI CASTG 1 cut(s) 546
XagI CCTNNNNNAGG 1 cut(s) 486
XapI RAATTY 2 cut(s) 23, 80
XhoI CTCGAG 1 cut(s) 417
XmnI GAANNNNTTC 1 cut(s) 441
XspI CTAG 2 cut(s) 222, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.