Rorug07G0284200

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0025182
Physical Location & Seq
Reverse (-)
27590653 .. 27593764
3112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0284200.1

Sequence Viewer

Length: 1167 bp
ATGCGACTGAACATGGCTGCTGGTATGGATTTTTCGCCTCCGACTACAATATTTGAAGGTGTTTGCTACAATGACAACATGTCAGCCTTGGAAGATGATGATGAGAATATCAACGGTTTGAAGCATATTGCGAATGGGAAACCTCCGAGGCATCTCTCGGTAATGCGGCACAGTTTGAGCTCCATGAGGATGCTCGCCCCTGAGGATTTGTTGGAAGTTGGGGTTGTAGCCAGTAAGTCACCTTCGAATGAGAGTTCAAACTTTCTCCCAGTGTTCCGATCAGGAAGCTGTGCTGAAAGAGGACCTAAACAGTACATGGAGGATGAACACGTTTGCGTAGATAATCTTGTTGAACATCTGGGTGGAACTTCTGCCTTCCCTTCACCTGGTGCTTTCTATGGGGTATTTGATGGCCATGGAGGCACAGATGCAGCATCGTTTATCAGAAGCAACATTCTTAGATTCATAGTTGAGGATTCTCATTTTCCAACTTGTTTGGAGAGGGCTATTAAGAGTGCCTACGTGAAAGCTGATCATGCATTTGCAGATGAAAGTTCTCTGGATATCTCCTCTGGCACCACTGCTCTGACTGCCCTTGTTTCGGGAAGGACCCTGATCATTGCTAATGCTGGAGACTGTCGAGCTGTAATGGGGAAACGGGGTAGAGCAATAGAGATGTCAAACGACCACAAACCAAATTGTGCATCAGAAAGGCTAAGAATTGAGAAACTTGGTGGTGTGATATATGACGGGTACCTCAATGGACAATTATCTGTGGCTCGTGCTCTAGGAGACTGGCACATGAAGGGACCGAAAGGCTCTGCCTGCCCTCTAAGTGCAGAGCCTGAGTTGAAGGAGACATGCCTGACTGAGGAAGATGAGTTTTTAATAATGGGGTGTGATGGTTTGTGGGATGTAATGAGTAGCCAATGTGCTGTAACGATGGCGAGGAAAGAACTGATGATCCATAATGATCCTGAAAGATGCTCAAGAGAACTGGTCAGAGAGGCACTCAAGCGGCACACATGTGATAATCTAACTGTTATCGTGGTTTGTTTTTCCCCAGATGCACCCCCTCGGATAGAAATACCTCAAAGCCGAGTCCGGAGGAGCATTTCTGCAGAAGGGCTTAATTTGTTGAAGGGTGTACTGGACTGCAACTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

388

Amino Acids

42.28

Weight (kDa)

5.43

Isoelectric Point (pI)

54.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 94 - 345 1.6e-72 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025182)

Species Orthologous Gene IDs
rosa_laevigata RLG00000001234
rosa_roxburghii Rroxscaffold_3G00226850
rosa_rugosa Rorug07G0284200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 753
AccB1I GGYRCC 2 cut(s) 575, 753
AccIII TCCGGA 1 cut(s) 1104
AciI CCGC 2 cut(s) 166, 1018
AclWI GGATC 2 cut(s) 958, 968
AcoI YGGCCR 1 cut(s) 412
AdeI CACNNNGTG 1 cut(s) 389
AfaI GTAC 3 cut(s) 314, 755, 1149
AfiI CCNNNNNNNGG 3 cut(s) 386, 601, 871
AflIII ACRYGT 3 cut(s) 78, 328, 1025
AgsI TTSAA 6 cut(s) 56, 121, 258, 353, 853, 1141
AhdI GACNNNNNGTC 1 cut(s) 79
AjnI CCWGG 1 cut(s) 385
AleI CACNNNNGTG 1 cut(s) 1026
AloI GAACNNNNNNTCC 2 cut(s) 948, 980
AluBI AGCT 4 cut(s) 180, 288, 530, 644
AluI AGCT 4 cut(s) 180, 288, 530, 644
Alw21I GWGCWC 2 cut(s) 182, 787
Alw26I GTCTC 3 cut(s) 627, 786, 851
AlwI GGATC 2 cut(s) 958, 968
AlwNI CAGNNNCTG 1 cut(s) 845
Aor13HI TCCGGA 1 cut(s) 1104
AoxI GGCC 1 cut(s) 412
ApeKI GCWGC 2 cut(s) 17, 431
Asp718I GGTACC 1 cut(s) 753
AspS9I GGNCC 3 cut(s) 302, 609, 809
AsuHPI GGTGA 2 cut(s) 231, 375
AsuII TTCGAA 1 cut(s) 245
AvaII GGWCC 3 cut(s) 302, 609, 809
AxyI CCTNAGG 1 cut(s) 201
BalI TGGCCA 1 cut(s) 414
BanI GGYRCC 2 cut(s) 575, 753
BanII GRGCYC 1 cut(s) 182
BarI GAAGNNNNNNTAC 2 cut(s) 226, 258
BauI CACGAG 1 cut(s) 780
Bbv12I GWGCWC 2 cut(s) 182, 787
BbvI GCAGC 2 cut(s) 4, 443
BccI CCATC 3 cut(s) 404, 896, 937
BciT130I CCWGG 1 cut(s) 387
BclI TGATCA 2 cut(s) 532, 615
BcoDI GTCTC 3 cut(s) 627, 786, 851
BfaI CTAG 1 cut(s) 788
BfmI CTRYAG 1 cut(s) 1119
BglI GCCNNNNNGGC 1 cut(s) 420
BisI GCNGC 4 cut(s) 18, 167, 432, 1019
BlsI GCNGC 4 cut(s) 19, 168, 433, 1020
Bme1390I CCNGG 1 cut(s) 387
Bme18I GGWCC 3 cut(s) 302, 609, 809
BmeRI GACNNNNNGTC 1 cut(s) 79
BmgT120I GGNCC 3 cut(s) 302, 609, 809
BmiI GGNNCC 4 cut(s) 577, 611, 755, 810
BmrFI CCNGG 1 cut(s) 387
BmrI ACTGGG 1 cut(s) 263
BmsI GCATC 7 cut(s) 160, 180, 418, 443, 713, 974, 1057
BmuI ACTGGG 1 cut(s) 263
BplI GAGNNNNNCTC 2 cut(s) 996, 1028
BpmI CTGGAG 1 cut(s) 651
Bpu14I TTCGAA 1 cut(s) 245
BpuEI CTTGAG 2 cut(s) 973, 998
BsaAI YACGTR 1 cut(s) 523
BsaJI CCNNGG 4 cut(s) 87, 146, 415, 1076
BsaWI WCCGGW 1 cut(s) 1104
Bsc4I CCNNNNNNNGG 3 cut(s) 386, 601, 871
Bse1I ACTGG 5 cut(s) 231, 269, 800, 1002, 1155
Bse21I CCTNAGG 1 cut(s) 201
Bse3DI GCAATG 1 cut(s) 618
BseAI TCCGGA 1 cut(s) 1104
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 4 cut(s) 87, 146, 415, 1076
BseGI GGATG 3 cut(s) 195, 328, 919
BseLI CCNNNNNNNGG 3 cut(s) 386, 601, 871
BseMI GCAATG 1 cut(s) 618
BseMII CTCAG 3 cut(s) 192, 837, 861
BseNI ACTGG 5 cut(s) 231, 269, 800, 1002, 1155
BseRI GAGGAG 2 cut(s) 559, 1123
BseXI GCAGC 2 cut(s) 4, 443
BsgI GTGCAG 1 cut(s) 858
BshFI GGCC 1 cut(s) 414
BshNI GGYRCC 2 cut(s) 575, 753
BsiHKAI GWGCWC 2 cut(s) 182, 787
BsiSI CCGG 1 cut(s) 1105
BslFI GGGAC 1 cut(s) 822
BslI CCNNNNNNNGG 3 cut(s) 386, 601, 871
BsmAI GTCTC 3 cut(s) 627, 786, 851
BsmFI GGGAC 1 cut(s) 822
BsnI GGCC 1 cut(s) 414
Bsp119I TTCGAA 1 cut(s) 245
Bsp1286I GDGCHC 2 cut(s) 182, 787
Bsp13I TCCGGA 1 cut(s) 1104
Bsp143I GATC 5 cut(s) 278, 532, 615, 963, 973
Bsp19I CCATGG 1 cut(s) 415
BspACI CCGC 2 cut(s) 166, 1018
BspANI GGCC 1 cut(s) 414
BspCNI CTCAG 3 cut(s) 193, 838, 862
BspEI TCCGGA 1 cut(s) 1104
BspHI TCATGA 1 cut(s) 1163
BspLI GGNNCC 4 cut(s) 577, 611, 755, 810
BspMAI CTGCAG 1 cut(s) 1123
BspPI GGATC 2 cut(s) 958, 968
BspT104I TTCGAA 1 cut(s) 245
BspT107I GGYRCC 2 cut(s) 575, 753
BsrDI GCAATG 1 cut(s) 618
BsrI ACTGG 5 cut(s) 231, 269, 800, 1002, 1155
BssECI CCNNGG 4 cut(s) 87, 146, 415, 1076
BssMI GATC 5 cut(s) 278, 532, 615, 963, 973
BssSI CACGAG 1 cut(s) 780
BssT1I CCWWGG 2 cut(s) 87, 415
Bst2BI CACGAG 1 cut(s) 780
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 5 cut(s) 116, 173, 312, 638, 1042
BstBAI YACGTR 1 cut(s) 523
BstBI TTCGAA 1 cut(s) 245
BstC8I GCNNGC 2 cut(s) 195, 826
BstDEI CTNAG 6 cut(s) 201, 458, 716, 833, 846, 870
BstDSI CCRYGG 1 cut(s) 415
BstENI CCTNNNNNAGG 1 cut(s) 869
BstF5I GGATG 3 cut(s) 195, 328, 919
BstKTI GATC 5 cut(s) 281, 535, 618, 966, 976
BstMAI GTCTC 3 cut(s) 627, 786, 851
BstMBI GATC 5 cut(s) 278, 532, 615, 963, 973
BstMWI GCNNNNNNNGC 4 cut(s) 420, 536, 590, 825
BstNI CCWGG 1 cut(s) 387
BstNSI RCATGY 3 cut(s) 82, 864, 1029
BstSCI CCNGG 1 cut(s) 385
BstSFI CTRYAG 1 cut(s) 1119
BstV1I GCAGC 2 cut(s) 4, 443
Bsu36I CCTNAGG 1 cut(s) 201
BsuRI GGCC 1 cut(s) 414
BtgI CCRYGG 1 cut(s) 415
BtsCI GGATG 3 cut(s) 195, 328, 919
BtsI GCAGTG 1 cut(s) 579
BtsIMutI CAGTG 2 cut(s) 276, 579
Cac8I GCNNGC 2 cut(s) 195, 826
CaiI CAGNNNCTG 1 cut(s) 845
CciI TCATGA 1 cut(s) 1163
Cfr13I GGNCC 3 cut(s) 302, 609, 809
CsiI ACCWGGT 1 cut(s) 385
Csp6I GTAC 3 cut(s) 313, 754, 1148
CviQI GTAC 3 cut(s) 313, 754, 1148
DdeI CTNAG 6 cut(s) 201, 458, 716, 833, 846, 870
DpnI GATC 5 cut(s) 280, 534, 617, 965, 975
DpnII GATC 5 cut(s) 278, 532, 615, 963, 973
DraIII CACNNNGTG 1 cut(s) 389
DriI GACNNNNNGTC 1 cut(s) 79
EaeI YGGCCR 1 cut(s) 412
Eam1105I GACNNNNNGTC 1 cut(s) 79
Ecl136II GAGCTC 1 cut(s) 180
Eco130I CCWWGG 2 cut(s) 87, 415
Eco24I GRGCYC 1 cut(s) 182
Eco32I GATATC 1 cut(s) 565
Eco47I GGWCC 3 cut(s) 302, 609, 809
Eco53kI GAGCTC 1 cut(s) 180
Eco81I CCTNAGG 1 cut(s) 201
EcoICRI GAGCTC 1 cut(s) 180
EcoNI CCTNNNNNAGG 1 cut(s) 869
EcoO109I RGGNCCY 2 cut(s) 302, 609
EcoRII CCWGG 1 cut(s) 385
EcoRV GATATC 1 cut(s) 565
EcoT14I CCWWGG 2 cut(s) 87, 415
EcoT22I ATGCAT 1 cut(s) 541
EcoT38I GRGCYC 1 cut(s) 182
ErhI CCWWGG 2 cut(s) 87, 415
FaqI GGGAC 1 cut(s) 822
FbaI TGATCA 2 cut(s) 532, 615
Fnu4HI GCNGC 4 cut(s) 18, 167, 432, 1019
FokI GGATG 3 cut(s) 202, 335, 926
FriOI GRGCYC 1 cut(s) 182
Fsp4HI GCNGC 4 cut(s) 18, 167, 432, 1019
FspBI CTAG 1 cut(s) 788
GluI GCNGC 4 cut(s) 18, 167, 432, 1019
GsuI CTGGAG 1 cut(s) 651
HaeIII GGCC 1 cut(s) 414
HapII CCGG 1 cut(s) 1105
HinfI GANTC 3 cut(s) 462, 476, 1101
HpaII CCGG 1 cut(s) 1105
HphI GGTGA 2 cut(s) 231, 375
Hpy166II GTNNAC 1 cut(s) 1148
Hpy188I TCNGA 8 cut(s) 42, 147, 278, 446, 588, 709, 1004, 1080
Hpy188III TCNNGA 7 cut(s) 282, 560, 603, 977, 990, 1105, 1164
Hpy8I GTNNAC 1 cut(s) 1148
HpyAV CCTTC 9 cut(s) 50, 252, 385, 390, 600, 799, 847, 1118, 1135
HpyCH4III ACNGT 5 cut(s) 116, 173, 312, 638, 1042
HpyCH4IV ACGT 2 cut(s) 330, 522
HpyCH4V TGCA 8 cut(s) 431, 539, 545, 704, 839, 1070, 1121, 1158
HpyF10VI GCNNNNNNNGC 4 cut(s) 420, 536, 590, 825
HpyF3I CTNAG 6 cut(s) 201, 458, 716, 833, 846, 870
HpySE526I ACGT 2 cut(s) 330, 522
Kpn2I TCCGGA 1 cut(s) 1104
KpnI GGTACC 1 cut(s) 757
Ksp22I TGATCA 2 cut(s) 532, 615
Kzo9I GATC 5 cut(s) 278, 532, 615, 963, 973
LmnI GCTCC 2 cut(s) 185, 1110
Lsp1109I GCAGC 2 cut(s) 4, 443
LweI GCATC 7 cut(s) 160, 180, 418, 443, 713, 974, 1057
MabI ACCWGGT 1 cut(s) 385
MaeI CTAG 1 cut(s) 788
MaeII ACGT 2 cut(s) 330, 522
MaeIII GTNAC 2 cut(s) 237, 937
MalI GATC 5 cut(s) 280, 534, 617, 965, 975
MboI GATC 5 cut(s) 278, 532, 615, 963, 973
MboII GAAGA 2 cut(s) 104, 887
MhlI GDGCHC 2 cut(s) 182, 787
MlsI TGGCCA 1 cut(s) 414
MluCI AATT 4 cut(s) 697, 720, 767, 1132
MluNI TGGCCA 1 cut(s) 414
MlyI GAGTC 1 cut(s) 1110
MmeI TCCRAC 3 cut(s) 65, 192, 512
Mox20I TGGCCA 1 cut(s) 414
Mph1103I ATGCAT 1 cut(s) 541
MroI TCCGGA 1 cut(s) 1104
MscI TGGCCA 1 cut(s) 414
MseI TTAA 3 cut(s) 510, 887, 1131
MslI CAYNNNNRTG 3 cut(s) 188, 360, 1026
Msp20I TGGCCA 1 cut(s) 414
MspI CCGG 1 cut(s) 1105
MspR9I CCNGG 1 cut(s) 387
MvaI CCWGG 1 cut(s) 387
MwoI GCNNNNNNNGC 4 cut(s) 420, 536, 590, 825
NcoI CCATGG 1 cut(s) 415
NdeII GATC 5 cut(s) 278, 532, 615, 963, 973
NlaIV GGNNCC 4 cut(s) 577, 611, 755, 810
NmeAIII GCCGAG 1 cut(s) 1124
NmuCI GTSAC 1 cut(s) 237
NsiI ATGCAT 1 cut(s) 541
NspI RCATGY 3 cut(s) 82, 864, 1029
NspV TTCGAA 1 cut(s) 245
OliI CACNNNNGTG 1 cut(s) 1026
PagI TCATGA 1 cut(s) 1163
PciI ACATGT 2 cut(s) 78, 1025
PfeI GAWTC 2 cut(s) 462, 476
PkrI GCNGC 4 cut(s) 19, 168, 433, 1020
PleI GAGTC 1 cut(s) 1109
PpsI GAGTC 1 cut(s) 1109
Ppu21I YACGTR 1 cut(s) 523
PpuMI RGGWCCY 2 cut(s) 302, 609
PscI ACATGT 2 cut(s) 78, 1025
Psp124BI GAGCTC 1 cut(s) 182
Psp5II RGGWCCY 2 cut(s) 302, 609
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 4 cut(s) 577, 611, 755, 810
PspPI GGNCC 3 cut(s) 302, 609, 809
PspPPI RGGWCCY 2 cut(s) 302, 609
PstI CTGCAG 1 cut(s) 1123
PstNI CAGNNNCTG 1 cut(s) 845
RsaI GTAC 3 cut(s) 314, 755, 1149
RsaNI GTAC 3 cut(s) 313, 754, 1148
RseI CAYNNNNRTG 3 cut(s) 188, 360, 1026
SacI GAGCTC 1 cut(s) 182
SaqAI TTAA 3 cut(s) 510, 887, 1131
SatI GCNGC 4 cut(s) 18, 167, 432, 1019
Sau3AI GATC 5 cut(s) 278, 532, 615, 963, 973
Sau96I GGNCC 3 cut(s) 302, 609, 809
SchI GAGTC 1 cut(s) 1110
ScrFI CCNGG 1 cut(s) 387
SduI GDGCHC 2 cut(s) 182, 787
SexAI ACCWGGT 1 cut(s) 385
SfaNI GCATC 7 cut(s) 160, 180, 418, 443, 713, 974, 1057
SfcI CTRYAG 1 cut(s) 1119
SfuI TTCGAA 1 cut(s) 245
SinI GGWCC 3 cut(s) 302, 609, 809
SmiMI CAYNNNNRTG 3 cut(s) 188, 360, 1026
SmlI CTYRAG 2 cut(s) 988, 1013
SmoI CTYRAG 2 cut(s) 988, 1013
Sse9I AATT 4 cut(s) 697, 720, 767, 1132
SsiI CCGC 2 cut(s) 166, 1018
SspI AATATT 1 cut(s) 51
SspMI CTAG 1 cut(s) 788
SstI GAGCTC 1 cut(s) 182
StyD4I CCNGG 1 cut(s) 385
StyI CCWWGG 2 cut(s) 87, 415
TaaI ACNGT 5 cut(s) 116, 173, 312, 638, 1042
TaiI ACGT 2 cut(s) 333, 525
TaqI TCGA 2 cut(s) 245, 640
TaqII GACCGA 1 cut(s) 826
TasI AATT 4 cut(s) 697, 720, 767, 1132
TatI WGTACW 2 cut(s) 312, 1147
TauI GCSGC 2 cut(s) 169, 1021
TfiI GAWTC 2 cut(s) 462, 476
Tru1I TTAA 3 cut(s) 510, 887, 1131
Tru9I TTAA 3 cut(s) 510, 887, 1131
TscAI CASTG 2 cut(s) 276, 586
TseFI GTSAC 1 cut(s) 237
TseI GCWGC 2 cut(s) 17, 431
Tsp45I GTSAC 1 cut(s) 237
TspDTI ATGAA 4 cut(s) 339, 454, 564, 818
TspRI CASTG 2 cut(s) 276, 586
VpaK11BI GGWCC 3 cut(s) 302, 609, 809
XagI CCTNNNNNAGG 1 cut(s) 869
XceI RCATGY 3 cut(s) 82, 864, 1029
XspI CTAG 1 cut(s) 788
Zsp2I ATGCAT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.