Rroxscaffold_3G00272610

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0025814
Physical Location & Seq
Forward (+)
64655190 .. 64662389
7200 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00272610.1

Sequence Viewer

Length: 738 bp
ATGGGTGTTAATTATAGAAAGACTGAAATAGAGTCCGAAAAGTGCATTGTTGGAACCGGGTTGGAATGCCAAGTGCCTTTAGATTCCGGGGTTCCCACTTCTTCCTATAACAGCCTCAACAAATCCGACTCATTTTTCGATGTAACCGCAAATGGGTTTACTCTCCTCAACAATTTCACCGCCTCCATCACCGCCGAAACTCTCACGTCATGCACCAGACAAACAAATCAATATACAACTTTTACTCACAAACTTTTCTCTTGCTATCTCATAGCTCGAAGTACGTCGTCAAACTCGCGAAGGATCAACAAGCACCAAGCAAACGTGCCGATCCATCCGCCATCAAAGCCATACTCGCCATGTGACATTGCTCATGGTTCAAATCTAATCAAGTTCAAGCCTCTGCAGCCCTTGATCATCCGTCGTCTTCAAGCTGATCGTCAACAAAACAAAGCTTCTACCAAGTTCTTCATCAATCTCGTGGAGAATCAACAAGCACCAAGCACACGTGCCGATTCATCCACCATCAAAGATAAAGAACACTTACCACGTGACACCGCTCGTGGTTCAAATCGATTAAGAGCAAGCCTCTCGCATCCCTTGATCAACCGCCTTTTACAAATCGTCAACAAAGTAAATCTTCTACAAGTTCTTCATCAAGCTCGTGGAGAATCAACAAGCACCAAACACACGTGCCGGTTTATCCCCTACCAAAGTCGAAGAACTCTCGCCATGTGA

Protein Analysis

245

Amino Acids

27.54

Weight (kDa)

9.95

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0025814)

Species Orthologous Gene IDs
rosa_roxburghii Rroxscaffold_3G00272610 Rroxscaffold_4G00315420
rosa_samantha Rh1DG155000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 560
AccII CGCG 1 cut(s) 298
AciI CCGC 6 cut(s) 147, 180, 192, 338, 558, 610
AclWI GGATC 2 cut(s) 311, 325
AcvI CACGTG 3 cut(s) 509, 551, 693
AfaI GTAC 1 cut(s) 283
AfiI CCNNNNNNNGG 1 cut(s) 153
AflIII ACRYGT 2 cut(s) 506, 690
AgsI TTSAA 4 cut(s) 381, 397, 431, 570
AjiI CACGTC 1 cut(s) 207
AluBI AGCT 4 cut(s) 275, 434, 455, 662
AluI AGCT 4 cut(s) 275, 434, 455, 662
AlwI GGATC 2 cut(s) 311, 325
ApeKI GCWGC 1 cut(s) 406
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
AsuC2I CCSGG 2 cut(s) 58, 88
AsuHPI GGTGA 2 cut(s) 169, 181
BauI CACGAG 3 cut(s) 479, 561, 663
BbrPI CACGTG 3 cut(s) 509, 551, 693
BbsI GAAGAC 1 cut(s) 419
BbvI GCAGC 1 cut(s) 418
BccI CCATC 4 cut(s) 194, 342, 349, 533
BcgI CGANNNNNNTGC 2 cut(s) 573, 607
BclI TGATCA 2 cut(s) 414, 603
BcnI CCSGG 2 cut(s) 58, 88
BfmI CTRYAG 1 cut(s) 404
BisI GCNGC 1 cut(s) 407
BlsI GCNGC 1 cut(s) 408
Bme1390I CCNGG 2 cut(s) 58, 88
BmgBI CACGTC 1 cut(s) 207
BmiI GGNNCC 2 cut(s) 55, 93
BmrFI CCNGG 2 cut(s) 58, 88
BmsI GCATC 1 cut(s) 604
BpiI GAAGAC 1 cut(s) 419
BplI GAGNNNNNCTC 2 cut(s) 573, 605
BpuMI CCSGG 2 cut(s) 58, 88
Bsa29I ATCGAT 1 cut(s) 574
BsaAI YACGTR 3 cut(s) 509, 551, 693
BsaJI CCNNGG 1 cut(s) 87
Bsc4I CCNNNNNNNGG 1 cut(s) 153
Bse118I RCCGGY 1 cut(s) 696
Bse3DI GCAATG 1 cut(s) 366
BseCI ATCGAT 1 cut(s) 574
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 4 cut(s) 334, 417, 518, 595
BseLI CCNNNNNNNGG 1 cut(s) 153
BseMI GCAATG 1 cut(s) 366
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 1 cut(s) 418
Bsh1236I CGCG 1 cut(s) 298
BshVI ATCGAT 1 cut(s) 574
BsiSI CCGG 3 cut(s) 57, 87, 697
BslI CCNNNNNNNGG 1 cut(s) 153
BsmI GAATGC 1 cut(s) 71
Bsp143I GATC 5 cut(s) 303, 330, 414, 436, 603
Bsp68I TCGCGA 1 cut(s) 298
BspACI CCGC 6 cut(s) 147, 180, 192, 338, 558, 610
BspDI ATCGAT 1 cut(s) 574
BspFNI CGCG 1 cut(s) 298
BspLI GGNNCC 2 cut(s) 55, 93
BspMAI CTGCAG 1 cut(s) 408
BspPI GGATC 2 cut(s) 311, 325
BsrBI CCGCTC 1 cut(s) 560
BsrDI GCAATG 1 cut(s) 366
BsrFI RCCGGY 1 cut(s) 696
BssAI RCCGGY 1 cut(s) 696
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 5 cut(s) 303, 330, 414, 436, 603
BssSI CACGAG 3 cut(s) 479, 561, 663
Bst2BI CACGAG 3 cut(s) 479, 561, 663
BstBAI YACGTR 3 cut(s) 509, 551, 693
BstC8I GCNNGC 1 cut(s) 586
BstF5I GGATG 4 cut(s) 334, 417, 518, 595
BstFNI CGCG 1 cut(s) 298
BstKTI GATC 5 cut(s) 306, 333, 417, 439, 606
BstMBI GATC 5 cut(s) 303, 330, 414, 436, 603
BstMWI GCNNNNNNNGC 3 cut(s) 346, 355, 406
BstSCI CCNGG 2 cut(s) 56, 86
BstSFI CTRYAG 1 cut(s) 404
BstUI CGCG 1 cut(s) 298
BstV1I GCAGC 1 cut(s) 418
BstV2I GAAGAC 1 cut(s) 419
Bsu15I ATCGAT 1 cut(s) 574
BsuTUI ATCGAT 1 cut(s) 574
BtrI CACGTC 1 cut(s) 207
BtsCI GGATG 4 cut(s) 334, 417, 518, 595
BtuMI TCGCGA 1 cut(s) 298
Cac8I GCNNGC 1 cut(s) 586
Cfr10I RCCGGY 1 cut(s) 696
ClaI ATCGAT 1 cut(s) 574
Csp6I GTAC 1 cut(s) 282
CviAII CATG 4 cut(s) 210, 360, 374, 733
CviJI RGCY 9 cut(s) 114, 275, 349, 400, 409, 434, 455, 588, 662
CviKI_1 RGCY 9 cut(s) 114, 275, 349, 400, 409, 434, 455, 588, 662
CviQI GTAC 1 cut(s) 282
DpnI GATC 5 cut(s) 305, 332, 416, 438, 605
DpnII GATC 5 cut(s) 303, 330, 414, 436, 603
EciI GGCGGA 1 cut(s) 327
Eco72I CACGTG 3 cut(s) 509, 551, 693
FaeI CATG 4 cut(s) 213, 363, 377, 736
FaiI YATR 9 cut(s) 15, 108, 211, 234, 272, 352, 361, 375, 734
FalI AAGNNNNNCTT 2 cut(s) 624, 656
FatI CATG 4 cut(s) 209, 359, 373, 732
FbaI TGATCA 2 cut(s) 414, 603
Fnu4HI GCNGC 1 cut(s) 407
FokI GGATG 4 cut(s) 321, 404, 505, 582
Fsp4HI GCNGC 1 cut(s) 407
GluI GCNGC 1 cut(s) 407
HapII CCGG 3 cut(s) 57, 87, 697
Hin1II CATG 4 cut(s) 213, 363, 377, 736
HincII GTYRAC 2 cut(s) 443, 628
HindII GTYRAC 2 cut(s) 443, 628
HindIII AAGCTT 1 cut(s) 453
HinfI GANTC 6 cut(s) 32, 83, 128, 487, 515, 671
HpaII CCGG 3 cut(s) 57, 87, 697
HphI GGTGA 2 cut(s) 169, 181
Hpy166II GTNNAC 3 cut(s) 159, 443, 628
Hpy188I TCNGA 2 cut(s) 37, 127
Hpy188III TCNNGA 1 cut(s) 297
Hpy8I GTNNAC 3 cut(s) 159, 443, 628
Hpy99I CGWCG 2 cut(s) 289, 426
HpyAV CCTTC 1 cut(s) 294
HpyCH4IV ACGT 6 cut(s) 206, 284, 324, 508, 550, 692
HpyCH4V TGCA 3 cut(s) 45, 213, 406
HpyF10VI GCNNNNNNNGC 3 cut(s) 346, 355, 406
HpySE526I ACGT 6 cut(s) 206, 284, 324, 508, 550, 692
Hsp92II CATG 4 cut(s) 213, 363, 377, 736
Ksp22I TGATCA 2 cut(s) 414, 603
Kzo9I GATC 5 cut(s) 303, 330, 414, 436, 603
LpnPI CCDG 4 cut(s) 70, 100, 229, 710
Lsp1109I GCAGC 1 cut(s) 418
LweI GCATC 1 cut(s) 604
MaeII ACGT 6 cut(s) 206, 284, 324, 508, 550, 692
MaeIII GTNAC 3 cut(s) 142, 362, 551
MalI GATC 5 cut(s) 305, 332, 416, 438, 605
MbiI CCGCTC 1 cut(s) 560
MboI GATC 5 cut(s) 303, 330, 414, 436, 603
MboII GAAGA 6 cut(s) 93, 419, 460, 632, 644, 732
MluCI AATT 2 cut(s) 10, 172
MlyI GAGTC 2 cut(s) 41, 122
MmeI TCCRAC 3 cut(s) 31, 42, 150
MnlI CCTC 5 cut(s) 125, 176, 193, 411, 599
MseI TTAA 2 cut(s) 9, 578
MspI CCGG 3 cut(s) 57, 87, 697
MspR9I CCNGG 2 cut(s) 58, 88
Mva1269I GAATGC 1 cut(s) 71
MvnI CGCG 1 cut(s) 298
MwoI GCNNNNNNNGC 3 cut(s) 346, 355, 406
NciI CCSGG 2 cut(s) 58, 88
NdeII GATC 5 cut(s) 303, 330, 414, 436, 603
NlaIII CATG 4 cut(s) 213, 363, 377, 736
NlaIV GGNNCC 2 cut(s) 55, 93
NmuCI GTSAC 2 cut(s) 362, 551
NruI TCGCGA 1 cut(s) 298
PctI GAATGC 1 cut(s) 71
PfeI GAWTC 4 cut(s) 83, 487, 515, 671
PkrI GCNGC 1 cut(s) 408
PleI GAGTC 2 cut(s) 40, 122
PmaCI CACGTG 3 cut(s) 509, 551, 693
PmlI CACGTG 3 cut(s) 509, 551, 693
PpsI GAGTC 2 cut(s) 40, 122
Ppu21I YACGTR 3 cut(s) 509, 551, 693
PspCI CACGTG 3 cut(s) 509, 551, 693
PspN4I GGNNCC 2 cut(s) 55, 93
PstI CTGCAG 1 cut(s) 408
RruI TCGCGA 1 cut(s) 298
RsaI GTAC 1 cut(s) 283
RsaNI GTAC 1 cut(s) 282
SaqAI TTAA 2 cut(s) 9, 578
SatI GCNGC 1 cut(s) 407
Sau3AI GATC 5 cut(s) 303, 330, 414, 436, 603
SchI GAGTC 2 cut(s) 41, 122
ScrFI CCNGG 2 cut(s) 58, 88
SfaNI GCATC 1 cut(s) 604
SfcI CTRYAG 1 cut(s) 404
Sse9I AATT 2 cut(s) 10, 172
SsiI CCGC 6 cut(s) 147, 180, 192, 338, 558, 610
StyD4I CCNGG 2 cut(s) 56, 86
TaiI ACGT 6 cut(s) 209, 287, 327, 511, 553, 695
TaqI TCGA 4 cut(s) 138, 277, 574, 718
TasI AATT 2 cut(s) 10, 172
TfiI GAWTC 4 cut(s) 83, 487, 515, 671
Tru1I TTAA 2 cut(s) 9, 578
Tru9I TTAA 2 cut(s) 9, 578
TseFI GTSAC 2 cut(s) 362, 551
TseI GCWGC 1 cut(s) 406
Tsp45I GTSAC 2 cut(s) 362, 551
TspDTI ATGAA 3 cut(s) 460, 507, 644
TspGWI ACGGA 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.