Rroxscaffold_4G00306030

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0022599
Physical Location & Seq
Reverse (-)
26828105 .. 26831736
3632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00306030.1

Sequence Viewer

Length: 294 bp
ATGGCAACATATGCTGAAGCAAATTATCAGCTCATAGCTGCTCCAAAGAAGAATGAAATAAGAGATATAGCAATGCATGAAGCAGTAAAGGGAAAACACTTCTTTCTTGAAACTGACATGAAGGGACCATCCTTGAAGCTTGACAACACTGGAAAAGCAATACTAACTGTCCTTCGTCACCTTGGTCGTGTAAGTGAGACACGGATTGGTCATCATCGGGAAACAAAAGAGTTGGCAATAGAAGATGCAAGGAAGGGAATACAAGTATGTCATGAAACTGATATGAAACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

97

Amino Acids

10.98

Weight (kDa)

8.76

Isoelectric Point (pI)

31.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 1 - 71 7.1e-24 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022599)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0349171
rosa_roxburghii Rroxscaffold_4G00306030
rosa_rugosa Rorug01G0201300
rosa_samantha Rh1AG218800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 36
AgsI TTSAA 2 cut(s) 110, 136
AluBI AGCT 3 cut(s) 31, 38, 139
AluI AGCT 3 cut(s) 31, 38, 139
Alw26I GTCTC 1 cut(s) 191
ApeKI GCWGC 1 cut(s) 38
AspS9I GGNCC 1 cut(s) 125
AsuHPI GGTGA 1 cut(s) 170
AvaII GGWCC 1 cut(s) 125
BbvI GCAGC 1 cut(s) 25
BccI CCATC 1 cut(s) 136
BcoDI GTCTC 1 cut(s) 191
BisI GCNGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 40
Bme18I GGWCC 1 cut(s) 125
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 1 cut(s) 126
BmsI GCATC 1 cut(s) 235
BsaJI CCNNGG 1 cut(s) 181
Bse1I ACTGG 1 cut(s) 154
Bse3DI GCAATG 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 181
BseGI GGATG 1 cut(s) 128
BseMI GCAATG 1 cut(s) 78
BseNI ACTGG 1 cut(s) 154
BseXI GCAGC 1 cut(s) 25
BslFI GGGAC 1 cut(s) 138
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 138
BspHI TCATGA 1 cut(s) 271
BspLI GGNNCC 1 cut(s) 126
BsrDI GCAATG 1 cut(s) 78
BsrI ACTGG 1 cut(s) 154
BssECI CCNNGG 1 cut(s) 181
BssT1I CCWWGG 1 cut(s) 181
Bst4CI ACNGT 1 cut(s) 169
BstAPI GCANNNNNTGC 1 cut(s) 11
BstF5I GGATG 1 cut(s) 128
BstMAI GTCTC 1 cut(s) 191
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstV1I GCAGC 1 cut(s) 25
BtsCI GGATG 1 cut(s) 128
BtsIMutI CAGTG 1 cut(s) 147
CciI TCATGA 1 cut(s) 271
Cfr13I GGNCC 1 cut(s) 125
CviAII CATG 3 cut(s) 77, 118, 272
CviJI RGCY 3 cut(s) 31, 38, 139
CviKI_1 RGCY 3 cut(s) 31, 38, 139
Eco130I CCWWGG 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 125
Eco57I CTGAAG 1 cut(s) 36
EcoT14I CCWWGG 1 cut(s) 181
EcoT22I ATGCAT 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 181
FaeI CATG 3 cut(s) 80, 121, 275
FaqI GGGAC 1 cut(s) 138
FatI CATG 3 cut(s) 76, 117, 271
FauNDI CATATG 1 cut(s) 10
Fnu4HI GCNGC 1 cut(s) 39
FokI GGATG 1 cut(s) 115
Fsp4HI GCNGC 1 cut(s) 39
GluI GCNGC 1 cut(s) 39
Hin1II CATG 3 cut(s) 80, 121, 275
HindIII AAGCTT 1 cut(s) 137
HphI GGTGA 1 cut(s) 170
Hpy188III TCNNGA 3 cut(s) 107, 218, 272
HpyAV CCTTC 3 cut(s) 115, 182, 247
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4V TGCA 2 cut(s) 76, 248
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
Hsp92II CATG 3 cut(s) 80, 121, 275
LmnI GCTCC 1 cut(s) 46
LpnPI CCDG 1 cut(s) 135
Lsp1109I GCAGC 1 cut(s) 25
LweI GCATC 1 cut(s) 235
MaeIII GTNAC 1 cut(s) 176
MboII GAAGA 2 cut(s) 61, 254
MluCI AATT 1 cut(s) 22
Mph1103I ATGCAT 1 cut(s) 78
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeI CATATG 1 cut(s) 10
NlaIII CATG 3 cut(s) 80, 121, 275
NlaIV GGNNCC 1 cut(s) 126
NmuCI GTSAC 1 cut(s) 176
NsiI ATGCAT 1 cut(s) 78
PagI TCATGA 1 cut(s) 271
PkrI GCNGC 1 cut(s) 40
PspN4I GGNNCC 1 cut(s) 126
PspPI GGNCC 1 cut(s) 125
SatI GCNGC 1 cut(s) 39
Sau96I GGNCC 1 cut(s) 125
SetI ASST 4 cut(s) 33, 40, 141, 183
SfaNI GCATC 1 cut(s) 235
SinI GGWCC 1 cut(s) 125
Sse9I AATT 1 cut(s) 22
StyI CCWWGG 1 cut(s) 181
TaaI ACNGT 1 cut(s) 169
TasI AATT 1 cut(s) 22
TscAI CASTG 1 cut(s) 154
TseFI GTSAC 1 cut(s) 176
TseI GCWGC 1 cut(s) 38
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 4 cut(s) 69, 93, 134, 288
TspGWI ACGGA 1 cut(s) 217
TspRI CASTG 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 125
Zsp2I ATGCAT 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.