Rorug01G0201300

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0022599
Physical Location & Seq
Forward (+)
28074851 .. 28075543
693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0201300.1

Sequence Viewer

Length: 693 bp
ATGCTGCTGCGCCGGAACCTCTCCCTCAAACCCTTCTCTCTCCTCCACTCAATCACCGGCGTTCCCTTCTCCACCTCCACCTCCGCCGTCGATTTCCTCCACTCCTACACCGTCACCCCTCCAATCCAAGACTGGCCCCACCACCTCCACCCAAAGCGCCTCATCTCCCTCATCACCCGCGAGCCCAAACTCGACCTCGCCCTCCAAATCTTCCACCACGCCTCCAAATACCACCCCGGATTTCGCCATAACTACCACACCTACCACGCCATCATCCGCCGCCTCCTCCGCTCCCGCGCTTTTCACCTCATCGACCCCCTCCTCTCCGACCTCCGCACCTCCAATCTCCGATGCAGCGAAGACCTCTTCATCTCCCTAATCCGCAACTACGGCGTCGTTTCACGCCCCAAACTCGCTTTCAAAACATTTATCACCATTCCGAAATTCGGCGTCCGACGATCCGTGAGGTCGTTGAATGCGCTGCTGAACGCTTTGGTTCAGAATCACGAGTACAGATTCGTTCACTCGGTGTTTCGGAACTGTAGGGAGAGGTTCGGGGTGGCGCCGAATGTGTTTAGTTGTAATATATTGATCAAAGCACTGTGTAAGAGAAATGATTTAGAGACTGCACGCCAGGTGCTCGATGAAATGCCTGCCATGGGATTTACACTAGAGTTTGATAGCTGTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

230

Amino Acids

26.64

Weight (kDa)

10.17

Isoelectric Point (pI)

50.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 186 - 217 9.4e-09 PPR repeat
PPR_2 PF13041 189 - 222 8.5e-08 PPR repeat family
PPR PF01535 192 - 222 9.9e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022599)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0349171
rosa_roxburghii Rroxscaffold_4G00306030
rosa_rugosa Rorug01G0201300
rosa_samantha Rh1AG218800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 562
AccBSI CCGCTC 1 cut(s) 291
AccII CGCG 2 cut(s) 180, 297
AciI CCGC 8 cut(s) 84, 178, 277, 280, 289, 295, 334, 382
AclWI GGATC 1 cut(s) 453
AcsI RAATTY 1 cut(s) 443
AcyI GRCGYC 3 cut(s) 393, 450, 563
AdeI CACNNNGTG 1 cut(s) 529
AfaI GTAC 1 cut(s) 512
AfiI CCNNNNNNNGG 2 cut(s) 446, 659
AgsI TTSAA 2 cut(s) 421, 475
AjnI CCWGG 1 cut(s) 633
AjuI GAANNNNNNNTTGG 2 cut(s) 401, 433
AluBI AGCT 1 cut(s) 684
AluI AGCT 1 cut(s) 684
Alw21I GWGCWC 1 cut(s) 642
Alw26I GTCTC 1 cut(s) 617
AlwI GGATC 1 cut(s) 453
AoxI GGCC 1 cut(s) 134
ApeKI GCWGC 4 cut(s) 4, 7, 354, 481
ApoI RAATTY 1 cut(s) 443
AspLEI GCGC 5 cut(s) 12, 159, 299, 481, 565
AspS9I GGNCC 1 cut(s) 135
AsuC2I CCSGG 1 cut(s) 237
AsuHPI GGTGA 5 cut(s) 46, 106, 166, 296, 424
BanI GGYRCC 1 cut(s) 562
BanII GRGCYC 1 cut(s) 186
BauI CACGAG 1 cut(s) 506
BbsI GAAGAC 1 cut(s) 366
Bbv12I GWGCWC 1 cut(s) 642
BbvI GCAGC 2 cut(s) 366, 468
BccI CCATC 1 cut(s) 278
BceAI ACGGC 2 cut(s) 71, 406
BciT130I CCWGG 1 cut(s) 635
BclI TGATCA 1 cut(s) 591
BcnI CCSGG 1 cut(s) 237
BcoDI GTCTC 1 cut(s) 617
BfaI CTAG 1 cut(s) 671
BfmI CTRYAG 1 cut(s) 541
BfoI RGCGCY 2 cut(s) 160, 566
BisI GCNGC 5 cut(s) 5, 8, 280, 355, 482
BlsI GCNGC 5 cut(s) 6, 9, 281, 356, 483
Bme1390I CCNGG 2 cut(s) 237, 635
BmgT120I GGNCC 1 cut(s) 135
BmiI GGNNCC 3 cut(s) 17, 137, 564
BmrFI CCNGG 2 cut(s) 237, 635
BmsI GCATC 1 cut(s) 341
BpiI GAAGAC 1 cut(s) 366
BpuMI CCSGG 1 cut(s) 237
BsaHI GRCGYC 3 cut(s) 393, 450, 563
BsaJI CCNNGG 2 cut(s) 235, 657
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 2 cut(s) 446, 659
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 137
BseBI CCWGG 1 cut(s) 635
BseDI CCNNGG 2 cut(s) 235, 657
BseGI GGATG 1 cut(s) 273
BseLI CCNNNNNNNGG 2 cut(s) 446, 659
BseNI ACTGG 1 cut(s) 137
BseRI GAGGAG 3 cut(s) 32, 275, 311
BseXI GCAGC 2 cut(s) 366, 468
BsgI GTGCAG 1 cut(s) 612
Bsh1236I CGCG 2 cut(s) 180, 297
BshFI GGCC 1 cut(s) 136
BshNI GGYRCC 1 cut(s) 562
BsiHKAI GWGCWC 1 cut(s) 642
BsiSI CCGG 3 cut(s) 13, 57, 237
BslI CCNNNNNNNGG 2 cut(s) 446, 659
BsmAI GTCTC 1 cut(s) 617
BsmI GAATGC 1 cut(s) 481
BsnI GGCC 1 cut(s) 136
Bsp1286I GDGCHC 2 cut(s) 186, 642
Bsp143I GATC 2 cut(s) 458, 591
Bsp19I CCATGG 1 cut(s) 657
BspACI CCGC 8 cut(s) 84, 178, 277, 280, 289, 295, 334, 382
BspANI GGCC 1 cut(s) 136
BspFNI CGCG 2 cut(s) 180, 297
BspLI GGNNCC 3 cut(s) 17, 137, 564
BspPI GGATC 1 cut(s) 453
BspT107I GGYRCC 1 cut(s) 562
BsrBI CCGCTC 1 cut(s) 291
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 1 cut(s) 137
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 2 cut(s) 235, 657
BssMI GATC 2 cut(s) 458, 591
BssNI GRCGYC 3 cut(s) 393, 450, 563
BssSI CACGAG 1 cut(s) 506
BssT1I CCWWGG 1 cut(s) 657
Bst2BI CACGAG 1 cut(s) 506
Bst2UI CCWGG 1 cut(s) 635
Bst4CI ACNGT 3 cut(s) 112, 542, 603
Bst6I CTCTTC 1 cut(s) 371
BstACI GRCGYC 3 cut(s) 393, 450, 563
BstC8I GCNNGC 3 cut(s) 182, 631, 654
BstDSI CCRYGG 1 cut(s) 657
BstF5I GGATG 1 cut(s) 273
BstFNI CGCG 2 cut(s) 180, 297
BstH2I RGCGCY 2 cut(s) 160, 566
BstHHI GCGC 5 cut(s) 12, 159, 299, 481, 565
BstKTI GATC 2 cut(s) 461, 594
BstMAI GTCTC 1 cut(s) 617
BstMBI GATC 2 cut(s) 458, 591
BstMWI GCNNNNNNNGC 2 cut(s) 288, 390
BstNI CCWGG 1 cut(s) 635
BstSCI CCNGG 2 cut(s) 235, 633
BstSFI CTRYAG 1 cut(s) 541
BstUI CGCG 2 cut(s) 180, 297
BstV1I GCAGC 2 cut(s) 366, 468
BstV2I GAAGAC 1 cut(s) 366
BsuRI GGCC 1 cut(s) 136
BtgI CCRYGG 1 cut(s) 657
BtsCI GGATG 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 599
Cac8I GCNNGC 3 cut(s) 182, 631, 654
CfoI GCGC 5 cut(s) 12, 159, 299, 481, 565
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 135
CseI GACGC 2 cut(s) 382, 439
Csp6I GTAC 1 cut(s) 511
CviAII CATG 2 cut(s) 658, 690
CviJI RGCY 3 cut(s) 136, 184, 684
CviKI_1 RGCY 3 cut(s) 136, 184, 684
CviQI GTAC 1 cut(s) 511
DinI GGCGCC 1 cut(s) 564
DpnI GATC 2 cut(s) 460, 593
DpnII GATC 2 cut(s) 458, 591
DraIII CACNNNGTG 1 cut(s) 529
Eam1104I CTCTTC 1 cut(s) 371
EarI CTCTTC 1 cut(s) 371
EciI GGCGGA 2 cut(s) 73, 266
Eco130I CCWWGG 1 cut(s) 657
Eco24I GRGCYC 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 633
EcoT14I CCWWGG 1 cut(s) 657
EcoT38I GRGCYC 1 cut(s) 186
EgeI GGCGCC 1 cut(s) 564
EheI GGCGCC 1 cut(s) 564
ErhI CCWWGG 1 cut(s) 657
FaeI CATG 2 cut(s) 661, 693
FaiI YATR 4 cut(s) 249, 587, 659, 691
FatI CATG 2 cut(s) 657, 689
FauI CCCGC 2 cut(s) 185, 302
FbaI TGATCA 1 cut(s) 591
Fnu4HI GCNGC 5 cut(s) 5, 8, 280, 355, 482
FokI GGATG 1 cut(s) 260
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 5 cut(s) 5, 8, 280, 355, 482
FspBI CTAG 1 cut(s) 671
GlaI GCGC 5 cut(s) 11, 158, 298, 480, 564
GluI GCNGC 5 cut(s) 5, 8, 280, 355, 482
HaeII RGCGCY 2 cut(s) 160, 566
HaeIII GGCC 1 cut(s) 136
HapII CCGG 3 cut(s) 13, 57, 237
HgaI GACGC 2 cut(s) 382, 439
HhaI GCGC 5 cut(s) 12, 159, 299, 481, 565
Hin1I GRCGYC 3 cut(s) 393, 450, 563
Hin1II CATG 2 cut(s) 661, 693
Hin6I GCGC 5 cut(s) 10, 157, 297, 479, 563
HinP1I GCGC 5 cut(s) 10, 157, 297, 479, 563
HinfI GANTC 2 cut(s) 502, 516
HpaII CCGG 3 cut(s) 13, 57, 237
HphI GGTGA 5 cut(s) 46, 106, 166, 296, 424
Hpy166II GTNNAC 1 cut(s) 523
Hpy188I TCNGA 6 cut(s) 328, 350, 441, 455, 501, 537
Hpy188III TCNNGA 1 cut(s) 506
Hpy8I GTNNAC 1 cut(s) 523
Hpy99I CGWCG 3 cut(s) 92, 398, 459
HpyAV CCTTC 2 cut(s) 43, 76
HpyCH4III ACNGT 3 cut(s) 112, 542, 603
HpyCH4V TGCA 3 cut(s) 354, 629, 689
HpyF10VI GCNNNNNNNGC 2 cut(s) 288, 390
Hsp92I GRCGYC 3 cut(s) 393, 450, 563
Hsp92II CATG 2 cut(s) 661, 693
HspAI GCGC 5 cut(s) 10, 157, 297, 479, 563
KasI GGCGCC 1 cut(s) 562
Ksp22I TGATCA 1 cut(s) 591
Kzo9I GATC 2 cut(s) 458, 591
LmnI GCTCC 1 cut(s) 296
LpnPI CCDG 7 cut(s) 26, 70, 118, 250, 620, 647, 666
Lsp1109I GCAGC 2 cut(s) 366, 468
LweI GCATC 1 cut(s) 341
MaeI CTAG 1 cut(s) 671
MaeIII GTNAC 1 cut(s) 112
MalI GATC 2 cut(s) 460, 593
MbiI CCGCTC 1 cut(s) 291
MboI GATC 2 cut(s) 458, 591
MboII GAAGA 3 cut(s) 202, 358, 371
MhlI GDGCHC 2 cut(s) 186, 642
MluCI AATT 1 cut(s) 443
Mly113I GGCGCC 1 cut(s) 563
MmeI TCCRAC 2 cut(s) 351, 478
MspI CCGG 3 cut(s) 13, 57, 237
MspR9I CCNGG 2 cut(s) 237, 635
Mva1269I GAATGC 1 cut(s) 481
MvaI CCWGG 1 cut(s) 635
MvnI CGCG 2 cut(s) 180, 297
MwoI GCNNNNNNNGC 2 cut(s) 288, 390
NarI GGCGCC 1 cut(s) 563
NciI CCSGG 1 cut(s) 237
NcoI CCATGG 1 cut(s) 657
NdeII GATC 2 cut(s) 458, 591
NlaIII CATG 2 cut(s) 661, 693
NlaIV GGNNCC 3 cut(s) 17, 137, 564
NmuCI GTSAC 1 cut(s) 112
PctI GAATGC 1 cut(s) 481
PfeI GAWTC 2 cut(s) 502, 516
PkrI GCNGC 5 cut(s) 6, 9, 281, 356, 483
PluTI GGCGCC 1 cut(s) 566
Psp6I CCWGG 1 cut(s) 633
PspGI CCWGG 1 cut(s) 633
PspN4I GGNNCC 3 cut(s) 17, 137, 564
PspPI GGNCC 1 cut(s) 135
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
SatI GCNGC 5 cut(s) 5, 8, 280, 355, 482
Sau3AI GATC 2 cut(s) 458, 591
Sau96I GGNCC 1 cut(s) 135
ScrFI CCNGG 2 cut(s) 237, 635
SduI GDGCHC 2 cut(s) 186, 642
SfaNI GCATC 1 cut(s) 341
SfcI CTRYAG 1 cut(s) 541
SfoI GGCGCC 1 cut(s) 564
SgrAI CRCCGGYG 1 cut(s) 56
Sse9I AATT 1 cut(s) 443
SsiI CCGC 8 cut(s) 84, 178, 277, 280, 289, 295, 334, 382
SspDI GGCGCC 1 cut(s) 562
SspMI CTAG 1 cut(s) 671
StyD4I CCNGG 2 cut(s) 235, 633
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 3 cut(s) 112, 542, 603
TaqI TCGA 4 cut(s) 90, 192, 312, 642
TasI AATT 1 cut(s) 443
TatI WGTACW 1 cut(s) 510
TauI GCSGC 1 cut(s) 282
TfiI GAWTC 2 cut(s) 502, 516
TscAI CASTG 1 cut(s) 606
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 4 cut(s) 4, 7, 354, 481
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 2 cut(s) 358, 660
TspGWI ACGGA 1 cut(s) 451
TspRI CASTG 1 cut(s) 606
XapI RAATTY 1 cut(s) 443
XcmI CCANNNNNNNNNTGG 1 cut(s) 129
XspI CTAG 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.