Rh1AG218800

Spindle and kinetochore-associated protein 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0022599
Physical Location & Seq
Forward (+)
41579809 .. 41580989
1181 bp
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UTR
Exon/CDS
Intron
Rh1AG218800.1

Sequence Viewer

Length: 294 bp
ATGAGAGGAAGGCTTACACTAGAGAAGGTCAATGCAGCTATTAGTGACATGGCAACATATGCTGAAGCAAATTATCAGCTCATAGCTGCTCCAAAGAAGAATGAAATAAGAGATATAGCAATGCATGAAGCAGTAAAGGGAAAACACTTCTTTCTTGAAACTGACATGAAGGGACCATCCTTGAAGCTTGACTACACTGGAAAAGCAATACTAACTGTCCTTCGTCACCTTGGTCGTGTAAGTGAGACACGGATTGGTCATCACCGGGTGATCATTCTTTTGAAGCCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SKA1

Protein Analysis

97

Amino Acids

10.96

Weight (kDa)

9.85

Isoelectric Point (pI)

22.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 1 - 87 3.5e-34 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022599)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0349171
rosa_roxburghii Rroxscaffold_4G00306030
rosa_rugosa Rorug01G0201300
rosa_samantha Rh1AG218800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 84
AdeI CACNNNGTG 1 cut(s) 268
AgsI TTSAA 3 cut(s) 158, 184, 283
AluBI AGCT 4 cut(s) 38, 79, 86, 187
AluI AGCT 4 cut(s) 38, 79, 86, 187
Alw26I GTCTC 1 cut(s) 239
ApeKI GCWGC 2 cut(s) 35, 86
AspS9I GGNCC 1 cut(s) 173
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 3 cut(s) 218, 254, 280
AvaII GGWCC 1 cut(s) 173
BarI GAAGNNNNNNTAC 2 cut(s) 176, 208
BbvI GCAGC 2 cut(s) 47, 73
BccI CCATC 1 cut(s) 184
BclI TGATCA 1 cut(s) 270
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 1 cut(s) 20
BisI GCNGC 2 cut(s) 36, 87
BlsI GCNGC 2 cut(s) 37, 88
Bme1390I CCNGG 1 cut(s) 266
Bme18I GGWCC 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 173
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 266
BpuMI CCSGG 1 cut(s) 266
BsaJI CCNNGG 1 cut(s) 229
Bse1I ACTGG 1 cut(s) 202
Bse3DI GCAATG 1 cut(s) 126
BseDI CCNNGG 1 cut(s) 229
BseGI GGATG 1 cut(s) 176
BseMI GCAATG 1 cut(s) 126
BseNI ACTGG 1 cut(s) 202
BseXI GCAGC 2 cut(s) 47, 73
BsiSI CCGG 1 cut(s) 265
BslFI GGGAC 1 cut(s) 186
BsmAI GTCTC 1 cut(s) 239
BsmFI GGGAC 1 cut(s) 186
Bsp143I GATC 1 cut(s) 270
BspLI GGNNCC 1 cut(s) 174
BsrDI GCAATG 1 cut(s) 126
BsrI ACTGG 1 cut(s) 202
BssECI CCNNGG 1 cut(s) 229
BssMI GATC 1 cut(s) 270
BssT1I CCWWGG 1 cut(s) 229
Bst4CI ACNGT 1 cut(s) 217
BstAPI GCANNNNNTGC 1 cut(s) 59
BstF5I GGATG 1 cut(s) 176
BstKTI GATC 1 cut(s) 273
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 1 cut(s) 270
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstSCI CCNGG 1 cut(s) 264
BstV1I GCAGC 2 cut(s) 47, 73
BtsCI GGATG 1 cut(s) 176
BtsIMutI CAGTG 2 cut(s) 195, 289
Cfr13I GGNCC 1 cut(s) 173
CviAII CATG 3 cut(s) 49, 125, 166
CviJI RGCY 6 cut(s) 13, 38, 79, 86, 187, 286
CviKI_1 RGCY 6 cut(s) 13, 38, 79, 86, 187, 286
DpnI GATC 1 cut(s) 272
DpnII GATC 1 cut(s) 270
DraIII CACNNNGTG 1 cut(s) 268
Eco130I CCWWGG 1 cut(s) 229
Eco47I GGWCC 1 cut(s) 173
Eco57I CTGAAG 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 229
EcoT22I ATGCAT 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 229
FaeI CATG 3 cut(s) 52, 128, 169
FaiI YATR 7 cut(s) 50, 58, 60, 83, 116, 126, 167
FaqI GGGAC 1 cut(s) 186
FatI CATG 3 cut(s) 48, 124, 165
FauNDI CATATG 1 cut(s) 58
FbaI TGATCA 1 cut(s) 270
Fnu4HI GCNGC 2 cut(s) 36, 87
FokI GGATG 1 cut(s) 163
Fsp4HI GCNGC 2 cut(s) 36, 87
FspBI CTAG 1 cut(s) 20
GluI GCNGC 2 cut(s) 36, 87
HapII CCGG 1 cut(s) 265
Hin1II CATG 3 cut(s) 52, 128, 169
HindIII AAGCTT 1 cut(s) 185
HpaII CCGG 1 cut(s) 265
HphI GGTGA 3 cut(s) 218, 254, 280
Hpy188III TCNNGA 1 cut(s) 155
HpyAV CCTTC 4 cut(s) 3, 19, 163, 230
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4V TGCA 2 cut(s) 35, 124
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
Hsp92II CATG 3 cut(s) 52, 128, 169
Ksp22I TGATCA 1 cut(s) 270
Kzo9I GATC 1 cut(s) 270
LmnI GCTCC 1 cut(s) 94
LpnPI CCDG 2 cut(s) 183, 278
Lsp1109I GCAGC 2 cut(s) 47, 73
MaeI CTAG 1 cut(s) 20
MaeIII GTNAC 2 cut(s) 44, 224
MalI GATC 1 cut(s) 272
MboI GATC 1 cut(s) 270
MboII GAAGA 1 cut(s) 109
MluCI AATT 1 cut(s) 70
Mph1103I ATGCAT 1 cut(s) 126
MspI CCGG 1 cut(s) 265
MspR9I CCNGG 1 cut(s) 266
MwoI GCNNNNNNNGC 1 cut(s) 59
NciI CCSGG 1 cut(s) 266
NdeI CATATG 1 cut(s) 58
NdeII GATC 1 cut(s) 270
NlaIII CATG 3 cut(s) 52, 128, 169
NlaIV GGNNCC 1 cut(s) 174
NmuCI GTSAC 2 cut(s) 44, 224
NsiI ATGCAT 1 cut(s) 126
PkrI GCNGC 2 cut(s) 37, 88
PspN4I GGNNCC 1 cut(s) 174
PspPI GGNCC 1 cut(s) 173
SatI GCNGC 2 cut(s) 36, 87
Sau3AI GATC 1 cut(s) 270
Sau96I GGNCC 1 cut(s) 173
ScrFI CCNGG 1 cut(s) 266
SetI ASST 6 cut(s) 30, 40, 81, 88, 189, 231
SinI GGWCC 1 cut(s) 173
Sse9I AATT 1 cut(s) 70
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 1 cut(s) 264
StyI CCWWGG 1 cut(s) 229
TaaI ACNGT 1 cut(s) 217
TasI AATT 1 cut(s) 70
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 2 cut(s) 44, 224
TseI GCWGC 2 cut(s) 35, 86
Tsp45I GTSAC 2 cut(s) 44, 224
TspDTI ATGAA 3 cut(s) 117, 141, 182
TspGWI ACGGA 1 cut(s) 265
TspRI CASTG 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 173
XspI CTAG 1 cut(s) 20
Zsp2I ATGCAT 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.