Rroxscaffold_4G00310870

Serine threonine-protein kinase GCN2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0025404
Physical Location & Seq
Forward (+)
33059885 .. 33061359
1475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00310870.1

Sequence Viewer

Length: 537 bp
ATGAGCGTAGATATGGATCTTCCGTCCGGCAGCAAACCTCAAGACGACGGAGAGGCCGGAGGCCACCGGGACGTCTGGGTGCCGCCGGATGAGACCAACTTTGTAACAACAGAGTGTCCACTCTCAGACGTTTTGATTCGAGACCCTATGTGCTTTGACAACCAGGTTGGTTTAAAAGTAGATTTATTTTTAAATAAATTTGAACACAAGAAACTTATTGCCTTCGGGTCTTTTATAAATTTTGACGTTTATCCACTGCAGGAAGCGAGGTTTCTTACTGACTTTGAAGAACTTGAGCCTCTTGGTATAGGTTTATTATCTGTTTCTCTTGCTTTTCCAAGTTCTCTTTTCCAGAAAGATGTTGGATCTATTCATTTTTCGTTAACTTCTCGATATATTTGTGTGCAACAAATGTCAGGAGAAGGTGGTTACGGGACAGTGGTTCGTTGTAAGTGCAATCTTGATGGTGGGATATATGCTGTGAAGAGAGTTAAGATTGGAGAAAACGGACTGCCTTATGAGACCGTAAGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

178

Amino Acids

19.79

Weight (kDa)

4.84

Isoelectric Point (pI)

34.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0025404)

Species Orthologous Gene IDs
rosa_laevigata RLG00000028986
rosa_roxburghii Rroxscaffold_4G00310870 Rroxscaffold_4G00310910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 236
AatII GACGTC 1 cut(s) 75
AccB1I GGYRCC 1 cut(s) 79
AciI CCGC 1 cut(s) 83
AclWI GGATC 2 cut(s) 24, 373
AcsI RAATTY 2 cut(s) 197, 238
AcyI GRCGYC 1 cut(s) 72
AfiI CCNNNNNNNGG 1 cut(s) 531
AgsI TTSAA 2 cut(s) 203, 287
AjnI CCWGG 1 cut(s) 162
Alw26I GTCTC 3 cut(s) 86, 135, 515
AlwI GGATC 2 cut(s) 24, 373
AoxI GGCC 2 cut(s) 54, 61
ApeKI GCWGC 1 cut(s) 30
ApoI RAATTY 2 cut(s) 197, 238
AsuC2I CCSGG 1 cut(s) 68
BanI GGYRCC 1 cut(s) 79
BbvI GCAGC 1 cut(s) 42
BccI CCATC 1 cut(s) 458
BciT130I CCWGG 1 cut(s) 164
BcnI CCSGG 1 cut(s) 68
BcoDI GTCTC 3 cut(s) 86, 135, 515
BfmI CTRYAG 1 cut(s) 257
BisI GCNGC 2 cut(s) 31, 83
BlsI GCNGC 2 cut(s) 32, 84
Bme1390I CCNGG 2 cut(s) 68, 164
BmiI GGNNCC 1 cut(s) 81
BmrFI CCNGG 2 cut(s) 68, 164
BpuEI CTTGAG 2 cut(s) 24, 314
BpuMI CCSGG 1 cut(s) 68
BsaBI GATNNNNATC 1 cut(s) 15
BsaHI GRCGYC 1 cut(s) 72
BsaI GGTCTC 3 cut(s) 86, 135, 515
Bsc4I CCNNNNNNNGG 1 cut(s) 531
Bse8I GATNNNNATC 1 cut(s) 15
BseBI CCWGG 1 cut(s) 164
BseGI GGATG 1 cut(s) 94
BseJI GATNNNNATC 1 cut(s) 15
BseLI CCNNNNNNNGG 1 cut(s) 531
BseMII CTCAG 1 cut(s) 138
BseXI GCAGC 1 cut(s) 42
BshFI GGCC 2 cut(s) 56, 63
BshNI GGYRCC 1 cut(s) 79
BsiSI CCGG 4 cut(s) 27, 57, 67, 86
BslFI GGGAC 2 cut(s) 83, 448
BslI CCNNNNNNNGG 1 cut(s) 531
BsmAI GTCTC 3 cut(s) 86, 135, 515
BsmFI GGGAC 2 cut(s) 83, 448
BsnI GGCC 2 cut(s) 56, 63
Bso31I GGTCTC 3 cut(s) 86, 135, 515
Bsp143I GATC 2 cut(s) 16, 365
BspACI CCGC 1 cut(s) 83
BspANI GGCC 2 cut(s) 56, 63
BspCNI CTCAG 1 cut(s) 137
BspLI GGNNCC 1 cut(s) 81
BspMAI CTGCAG 1 cut(s) 261
BspPI GGATC 2 cut(s) 24, 373
BspT107I GGYRCC 1 cut(s) 79
BspTNI GGTCTC 3 cut(s) 86, 135, 515
BssMI GATC 2 cut(s) 16, 365
BssNI GRCGYC 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 2 cut(s) 439, 526
Bst6I CTCTTC 1 cut(s) 479
BstACI GRCGYC 1 cut(s) 72
BstDEI CTNAG 1 cut(s) 124
BstF5I GGATG 1 cut(s) 94
BstKTI GATC 2 cut(s) 19, 368
BstMAI GTCTC 3 cut(s) 86, 135, 515
BstMBI GATC 2 cut(s) 16, 365
BstNI CCWGG 1 cut(s) 164
BstSCI CCNGG 2 cut(s) 66, 162
BstSFI CTRYAG 1 cut(s) 257
BstV1I GCAGC 1 cut(s) 42
BstX2I RGATCY 2 cut(s) 16, 365
BstYI RGATCY 2 cut(s) 16, 365
BsuRI GGCC 2 cut(s) 56, 63
BtsCI GGATG 1 cut(s) 94
BtsI GCAGTG 1 cut(s) 254
BtsIMutI CAGTG 2 cut(s) 254, 444
CsiI ACCWGGT 1 cut(s) 162
CviJI RGCY 3 cut(s) 56, 63, 298
CviKI_1 RGCY 3 cut(s) 56, 63, 298
DdeI CTNAG 1 cut(s) 124
DpnI GATC 2 cut(s) 18, 367
DpnII GATC 2 cut(s) 16, 365
DraI TTTAAA 2 cut(s) 174, 192
Eam1104I CTCTTC 1 cut(s) 479
EarI CTCTTC 1 cut(s) 479
Eco31I GGTCTC 3 cut(s) 86, 135, 515
EcoRII CCWGG 1 cut(s) 162
FaiI YATR 8 cut(s) 14, 149, 236, 308, 396, 475, 477, 519
FaqI GGGAC 2 cut(s) 83, 448
Fnu4HI GCNGC 2 cut(s) 31, 83
FokI GGATG 1 cut(s) 101
Fsp4HI GCNGC 2 cut(s) 31, 83
GluI GCNGC 2 cut(s) 31, 83
HaeIII GGCC 2 cut(s) 56, 63
HapII CCGG 4 cut(s) 27, 57, 67, 86
Hin1I GRCGYC 1 cut(s) 72
HincII GTYRAC 1 cut(s) 384
HindII GTYRAC 1 cut(s) 384
HinfI GANTC 1 cut(s) 136
HpaI GTTAAC 1 cut(s) 384
HpaII CCGG 4 cut(s) 27, 57, 67, 86
Hpy166II GTNNAC 2 cut(s) 119, 384
Hpy188I TCNGA 1 cut(s) 127
Hpy188III TCNNGA 6 cut(s) 41, 140, 352, 390, 417, 461
Hpy8I GTNNAC 2 cut(s) 119, 384
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 2 cut(s) 232, 416
HpyCH4III ACNGT 2 cut(s) 439, 526
HpyCH4IV ACGT 3 cut(s) 72, 129, 246
HpyCH4V TGCA 3 cut(s) 259, 406, 456
HpyF3I CTNAG 1 cut(s) 124
HpySE526I ACGT 3 cut(s) 72, 129, 246
Hsp92I GRCGYC 1 cut(s) 72
KspAI GTTAAC 1 cut(s) 384
Kzo9I GATC 2 cut(s) 16, 365
Lsp1109I GCAGC 1 cut(s) 42
MabI ACCWGGT 1 cut(s) 162
MaeII ACGT 3 cut(s) 72, 129, 246
MaeIII GTNAC 2 cut(s) 103, 428
MalI GATC 2 cut(s) 18, 367
MboI GATC 2 cut(s) 16, 365
MboII GAAGA 3 cut(s) 11, 299, 496
MflI RGATCY 2 cut(s) 16, 365
MluCI AATT 2 cut(s) 197, 238
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 6 cut(s) 46, 48, 53, 261, 309, 525
MseI TTAA 4 cut(s) 173, 191, 383, 492
MspI CCGG 4 cut(s) 27, 57, 67, 86
MspR9I CCNGG 2 cut(s) 68, 164
MvaI CCWGG 1 cut(s) 164
NciI CCSGG 1 cut(s) 68
NdeII GATC 2 cut(s) 16, 365
NlaIV GGNNCC 1 cut(s) 81
PfeI GAWTC 1 cut(s) 136
PkrI GCNGC 2 cut(s) 32, 84
PsiI TTATAA 1 cut(s) 236
Psp6I CCWGG 1 cut(s) 162
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 1 cut(s) 81
PstI CTGCAG 1 cut(s) 261
PsuI RGATCY 2 cut(s) 16, 365
SaqAI TTAA 4 cut(s) 173, 191, 383, 492
SatI GCNGC 2 cut(s) 31, 83
Sau3AI GATC 2 cut(s) 16, 365
ScrFI CCNGG 2 cut(s) 68, 164
SetI ASST 9 cut(s) 40, 75, 132, 168, 249, 272, 313, 427, 536
SexAI ACCWGGT 1 cut(s) 162
SfcI CTRYAG 1 cut(s) 257
SmlI CTYRAG 2 cut(s) 39, 293
SmoI CTYRAG 2 cut(s) 39, 293
Sse9I AATT 2 cut(s) 197, 238
SsiI CCGC 1 cut(s) 83
StyD4I CCNGG 2 cut(s) 66, 162
TaaI ACNGT 2 cut(s) 439, 526
TaiI ACGT 3 cut(s) 75, 132, 249
TaqI TCGA 2 cut(s) 139, 391
TasI AATT 2 cut(s) 197, 238
TauI GCSGC 1 cut(s) 85
TfiI GAWTC 1 cut(s) 136
Tru1I TTAA 4 cut(s) 173, 191, 383, 492
Tru9I TTAA 4 cut(s) 173, 191, 383, 492
TscAI CASTG 2 cut(s) 261, 444
TseI GCWGC 1 cut(s) 30
TspDTI ATGAA 1 cut(s) 362
TspGWI ACGGA 3 cut(s) 12, 63, 522
TspRI CASTG 2 cut(s) 261, 444
XapI RAATTY 2 cut(s) 197, 238
XcmI CCANNNNNNNNNTGG 1 cut(s) 359
ZraI GACGTC 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.