Rroxscaffold_4G00310910

Serine threonine-protein kinase GCN2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0025404
Physical Location & Seq
Forward (+)
33085891 .. 33087352
1462 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_4G00310910.1

Sequence Viewer

Length: 525 bp
ATGGATCTTCCGTCCGGCAGCAAACCTCAAGACGACGGAGAGGCCGGAGGCCACCAGGACGTCCGGGTGCCGCCGGATGAGACCAACTTTGTAACAACAGAGTGTCCACTCTCAGACGTTTTGATTCGAGACCCTATGTGCTTTGACAACCAGGTTGGTTTAAAAGTAGATTTATTTTTAAATAAATTTGAACACAAGAAACTTATTGCCTTCGGGTCTTTTATAAATTTTGACGTTTATCCACTGCAGGAAGCGAGGTTTCTTACTGACTTTGAAGAACTTGAGCCTCTTGGTATAGGTTTATTATCTGTTTCTCTTGCTTTTCCAAGTTCTCTTTTCCAGAAAGATGTTGGATCTATTCATTTTTCGTTAACTTCTCGATATATTTGTGTGCAACAAATGTCAGGAGAAGGTGGTTACGGGACAGTGGTTCGTTGTAAGTGCAATCTTGATGGTGGGATATATGCTGTGAAGAGAGTTAAGATTGGAGAAAACGGACTGCCTTATGAGACCGTAAGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

174

Amino Acids

19.3

Weight (kDa)

4.93

Isoelectric Point (pI)

34.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025404)

Species Orthologous Gene IDs
rosa_laevigata RLG00000028986
rosa_roxburghii Rroxscaffold_4G00310870 Rroxscaffold_4G00310910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 224
AatII GACGTC 1 cut(s) 63
AccB1I GGYRCC 1 cut(s) 67
AciI CCGC 1 cut(s) 71
AclWI GGATC 2 cut(s) 12, 361
AcsI RAATTY 2 cut(s) 185, 226
AcyI GRCGYC 1 cut(s) 60
AfiI CCNNNNNNNGG 1 cut(s) 519
AgsI TTSAA 2 cut(s) 191, 275
AjnI CCWGG 2 cut(s) 54, 150
Alw26I GTCTC 3 cut(s) 74, 123, 503
AlwI GGATC 2 cut(s) 12, 361
AoxI GGCC 2 cut(s) 42, 49
ApeKI GCWGC 1 cut(s) 18
ApoI RAATTY 2 cut(s) 185, 226
AsuC2I CCSGG 1 cut(s) 65
BanI GGYRCC 1 cut(s) 67
BbvI GCAGC 1 cut(s) 30
BccI CCATC 1 cut(s) 446
BciT130I CCWGG 2 cut(s) 56, 152
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 3 cut(s) 74, 123, 503
BfmI CTRYAG 1 cut(s) 245
BisI GCNGC 2 cut(s) 19, 71
BlsI GCNGC 2 cut(s) 20, 72
Bme1390I CCNGG 3 cut(s) 56, 65, 152
BmiI GGNNCC 1 cut(s) 69
BmrFI CCNGG 3 cut(s) 56, 65, 152
BpuEI CTTGAG 2 cut(s) 12, 302
BpuMI CCSGG 1 cut(s) 65
BsaHI GRCGYC 1 cut(s) 60
BsaI GGTCTC 3 cut(s) 74, 123, 503
Bsc4I CCNNNNNNNGG 1 cut(s) 519
BseBI CCWGG 2 cut(s) 56, 152
BseGI GGATG 1 cut(s) 82
BseLI CCNNNNNNNGG 1 cut(s) 519
BseMII CTCAG 1 cut(s) 126
BseXI GCAGC 1 cut(s) 30
BshFI GGCC 2 cut(s) 44, 51
BshNI GGYRCC 1 cut(s) 67
BsiSI CCGG 4 cut(s) 15, 45, 64, 74
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 1 cut(s) 519
BsmAI GTCTC 3 cut(s) 74, 123, 503
BsmFI GGGAC 1 cut(s) 436
BsnI GGCC 2 cut(s) 44, 51
Bso31I GGTCTC 3 cut(s) 74, 123, 503
Bsp143I GATC 2 cut(s) 4, 353
BspACI CCGC 1 cut(s) 71
BspANI GGCC 2 cut(s) 44, 51
BspCNI CTCAG 1 cut(s) 125
BspLI GGNNCC 1 cut(s) 69
BspMAI CTGCAG 1 cut(s) 249
BspPI GGATC 2 cut(s) 12, 361
BspT107I GGYRCC 1 cut(s) 67
BspTNI GGTCTC 3 cut(s) 74, 123, 503
BssMI GATC 2 cut(s) 4, 353
BssNI GRCGYC 1 cut(s) 60
Bst2UI CCWGG 2 cut(s) 56, 152
Bst4CI ACNGT 2 cut(s) 427, 514
Bst6I CTCTTC 1 cut(s) 467
BstACI GRCGYC 1 cut(s) 60
BstDEI CTNAG 1 cut(s) 112
BstF5I GGATG 1 cut(s) 82
BstKTI GATC 2 cut(s) 7, 356
BstMAI GTCTC 3 cut(s) 74, 123, 503
BstMBI GATC 2 cut(s) 4, 353
BstNI CCWGG 2 cut(s) 56, 152
BstSCI CCNGG 3 cut(s) 54, 63, 150
BstSFI CTRYAG 1 cut(s) 245
BstV1I GCAGC 1 cut(s) 30
BstX2I RGATCY 2 cut(s) 4, 353
BstYI RGATCY 2 cut(s) 4, 353
BsuRI GGCC 2 cut(s) 44, 51
BtsCI GGATG 1 cut(s) 82
BtsI GCAGTG 1 cut(s) 242
BtsIMutI CAGTG 2 cut(s) 242, 432
CsiI ACCWGGT 1 cut(s) 150
CviJI RGCY 3 cut(s) 44, 51, 286
CviKI_1 RGCY 3 cut(s) 44, 51, 286
DdeI CTNAG 1 cut(s) 112
DpnI GATC 2 cut(s) 6, 355
DpnII GATC 2 cut(s) 4, 353
DraI TTTAAA 2 cut(s) 162, 180
Eam1104I CTCTTC 1 cut(s) 467
EarI CTCTTC 1 cut(s) 467
Eco31I GGTCTC 3 cut(s) 74, 123, 503
EcoRII CCWGG 2 cut(s) 54, 150
FaiI YATR 7 cut(s) 137, 224, 296, 384, 463, 465, 507
FaqI GGGAC 1 cut(s) 436
Fnu4HI GCNGC 2 cut(s) 19, 71
FokI GGATG 1 cut(s) 89
Fsp4HI GCNGC 2 cut(s) 19, 71
GluI GCNGC 2 cut(s) 19, 71
HaeIII GGCC 2 cut(s) 44, 51
HapII CCGG 4 cut(s) 15, 45, 64, 74
Hin1I GRCGYC 1 cut(s) 60
HincII GTYRAC 1 cut(s) 372
HindII GTYRAC 1 cut(s) 372
HinfI GANTC 1 cut(s) 124
HpaI GTTAAC 1 cut(s) 372
HpaII CCGG 4 cut(s) 15, 45, 64, 74
Hpy166II GTNNAC 2 cut(s) 107, 372
Hpy188I TCNGA 1 cut(s) 115
Hpy188III TCNNGA 6 cut(s) 29, 128, 340, 378, 405, 449
Hpy8I GTNNAC 2 cut(s) 107, 372
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 2 cut(s) 220, 404
HpyCH4III ACNGT 2 cut(s) 427, 514
HpyCH4IV ACGT 3 cut(s) 60, 117, 234
HpyCH4V TGCA 3 cut(s) 247, 394, 444
HpyF3I CTNAG 1 cut(s) 112
HpySE526I ACGT 3 cut(s) 60, 117, 234
Hsp92I GRCGYC 1 cut(s) 60
KspAI GTTAAC 1 cut(s) 372
Kzo9I GATC 2 cut(s) 4, 353
Lsp1109I GCAGC 1 cut(s) 30
MabI ACCWGGT 1 cut(s) 150
MaeII ACGT 3 cut(s) 60, 117, 234
MaeIII GTNAC 2 cut(s) 91, 416
MalI GATC 2 cut(s) 6, 355
MboI GATC 2 cut(s) 4, 353
MboII GAAGA 2 cut(s) 287, 484
MflI RGATCY 2 cut(s) 4, 353
MluCI AATT 2 cut(s) 185, 226
MmeI TCCRAC 1 cut(s) 331
MnlI CCTC 6 cut(s) 34, 36, 41, 249, 297, 513
MseI TTAA 4 cut(s) 161, 179, 371, 480
MspI CCGG 4 cut(s) 15, 45, 64, 74
MspR9I CCNGG 3 cut(s) 56, 65, 152
MvaI CCWGG 2 cut(s) 56, 152
NciI CCSGG 1 cut(s) 65
NdeII GATC 2 cut(s) 4, 353
NlaIV GGNNCC 1 cut(s) 69
PfeI GAWTC 1 cut(s) 124
PkrI GCNGC 2 cut(s) 20, 72
PsiI TTATAA 1 cut(s) 224
Psp6I CCWGG 2 cut(s) 54, 150
PspGI CCWGG 2 cut(s) 54, 150
PspN4I GGNNCC 1 cut(s) 69
PstI CTGCAG 1 cut(s) 249
PsuI RGATCY 2 cut(s) 4, 353
SaqAI TTAA 4 cut(s) 161, 179, 371, 480
SatI GCNGC 2 cut(s) 19, 71
Sau3AI GATC 2 cut(s) 4, 353
ScrFI CCNGG 3 cut(s) 56, 65, 152
SetI ASST 9 cut(s) 28, 63, 120, 156, 237, 260, 301, 415, 524
SexAI ACCWGGT 1 cut(s) 150
SfcI CTRYAG 1 cut(s) 245
SmlI CTYRAG 2 cut(s) 27, 281
SmoI CTYRAG 2 cut(s) 27, 281
Sse9I AATT 2 cut(s) 185, 226
SsiI CCGC 1 cut(s) 71
StyD4I CCNGG 3 cut(s) 54, 63, 150
TaaI ACNGT 2 cut(s) 427, 514
TaiI ACGT 3 cut(s) 63, 120, 237
TaqI TCGA 2 cut(s) 127, 379
TasI AATT 2 cut(s) 185, 226
TauI GCSGC 1 cut(s) 73
TfiI GAWTC 1 cut(s) 124
Tru1I TTAA 4 cut(s) 161, 179, 371, 480
Tru9I TTAA 4 cut(s) 161, 179, 371, 480
TscAI CASTG 2 cut(s) 249, 432
TseI GCWGC 1 cut(s) 18
TspDTI ATGAA 1 cut(s) 350
TspGWI ACGGA 2 cut(s) 51, 510
TspRI CASTG 2 cut(s) 249, 432
XapI RAATTY 2 cut(s) 185, 226
XcmI CCANNNNNNNNNTGG 1 cut(s) 347
ZraI GACGTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.