Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0005718

Physical Location & Seq

Genomic Coordinates Forward (+)

39144380 .. 39148529

Gene Structure 4150 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00314920.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1551 bp

ATGGTTTCAGAGTCGAGCAGCAGTACAAGTAGCTGCCCCATCAAAACGGTGGTGGTTCTGGTCCAAGAGAACCGCTCCTTCGACCACATGCTCGGGTGGATGAAGTCCCTAAACCCTGACATCAACGGCGTCACCGAATCCGAGTCAAACCTCATTTCCACCTCCGACCCTAACTCCAAACGTGTCTTCTTCGGCGACAACTCCGCCTACGTCGACCCTGACCCAGGCCACTCCATCCAAGCTGTCTACGAGCAAGTCTTCGGCGAGCCATGGTCGGAGGCCTCGGCGTCCAAGAACCTCGCCCCAACAATGGAGGGATTCGCTCAAAACGCCGAGACAACGCAGGCGGGGTTGGCTGAGACGGTGATGAACGGGTTCAAGCCGGAGAACATTCCAGTGTATAGGGAGCTGGTGAAAGAGTTTGCAGTGTGTGACAGGTGGTTTGCTTCTGTGCCGGCGTCGACGCAGCCGAACCGGCAGTACGTGCACTCGGCGACGTCGCATGGACTTTCCGGGACTGATACGAAGTTGCTGATTGAAGGGCTGCCACAGAAGACTATATTTGAGTCCATGGAGGAGGCTGGCCATTCTTTTGGAATTTACTATCAGACCCCTCCTGCCACACTTTTGTACAGGAATCTAAGAAAGTTGAAGTACATCGATAACTTCCATCAGTTTGATCTACATTTCAAGAAGCACTGCGAGGAGGGCAAATTACCAAACTATACAGTTGTGGAACAAAGATTCTTCGATCTATTGTCCCTCCCTGCCAACGATGATCACCCGTCTCATGACGTCTCAGAGGGCCAAAAATTCGTAAAGCAAGTGTACGAAGCATTACGATCAAGTCCTCAGTGGAATGAAATGTTGTTCATAATTGTATACGACGAGCATGGCGGATTCTATGATCATGTTCCGACGCCTGTTAAAGATGTTCCTAGTCCTGATGACATCACCGGTCCTGCACCCTACAACTTTAAATTCGATCGTCTTGGTGTTAGGGTTCCGGCCTTCCTAATTTCTCCCTGGATTGAGCGTGGAACAGTGTTGCATGGGCCTTCGGGGCCATATCCTTCTTCAGAGTTTGAGCACTCCTCAATTGCAGCAACTGTTAAGAAGATTTTCAATCTAAAAGAGTTTCTCACAAAGCGTGATGCTTGGGCAGGAACCTTTGATGTTATTTTGACTAGAACAACTCCAAGAACTGATTGCCCAGTTACTTTACCCGAGCCAATCAAACTACGAGAAGCTGGATCTAAAGATGATGCAAAGATCAGCGACTTTCAAGAAGAACTAGTACAATTGGCTGCAGCACTAAACGGGGATCACAGGAAGGATATATATCCCCACAAACTGGTTGAGAATATGATTGTTTCAGAGGCTCTCAAATATGTGGAAGGAGCGTTTAAAATGTTTCAAGATGAATGTCAAAAAGCCCGGGAAAGTGGAACTGACGAATCTCAAATTATCGTCTGCGCAACCTCTCCTATGCCGCAATCATCAAAGTCATTTATTCATAAGATATTCTCTTGTTTGGTTTGTGATAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003993 GO:0004620 GO:0004629 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005911 GO:0006629 GO:0006644 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0008081 GO:0008150 GO:0008152 GO:0009056 GO:0009267 GO:0009395 GO:0009405 GO:0009506 GO:0009605 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016042 GO:0016298 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0030054 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0034480 GO:0035821 GO:0042578 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044003 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0046434 GO:0050896 GO:0051701 GO:0051704 GO:0051716 GO:0051817 GO:0052008 GO:0052043 GO:0052111 GO:0052185 GO:0052188 GO:0052368 GO:0052642 GO:0055044 GO:0071496 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00562 ko00564 ko00565 ko01100 ko01110 ko02024 ko04919 map00562 map00564 map00565 map01100 map01110 map02024 map04919

Pfam Domains

Protein Families

Phosphoesterase

Protein Analysis

516

Amino Acids

57.84

Weight (kDa)

5.32

Isoelectric Point (pI)

44.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phosphoesterase	PF04185	14 - 378	5.2e-107	Phosphoesterase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005718)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03520 AT3G03520 AT3G03530
fragaria_vesca	FvH4_7g07140
malus_domestica	MD07G1077400.v1.1 MD07G1077500.v1.1
prunus_persica	Prupe.2G098600_v2.0.a1 Prupe.2G098700_v2.0.a1
pyrus_communis	pycom07g06100 pycom07g06110
rosa_chinensis	RchiOBHm_Chr1g0337121 RchiOBHm_Chr1g0337171
rosa_laevigata	RLG00000029302 RLG00000029303
rosa_multiflora	Rmu_sc0002160.1_g000021
rosa_roxburghii	Rroxscaffold_4G00314910 Rroxscaffold_4G00314920
rosa_rugosa	Rorug01G0131900.1
rosa_samantha	Rh1AG152000 Rh1AG152500 Rh1BG121200 Rh1BG121300 Rh1CG143200 Rh1CG143500 Rh1DG159200 Rh1DG159800
rosa_wichuraiana	Rw1G012440 Rw1G012460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	500, 798
Acc16I	TGCGCA	1 cut(s)	1477
AccB7I	CCANNNNNTGG	1 cut(s)	1354
AccBSI	CCGCTC	1 cut(s)	75
AccI	GTMKAC	4 cut(s)	213, 246, 461, 882
AciI	CCGC	5 cut(s)	73, 204, 347, 897, 1493
AclWI	GGATC	2 cut(s)	1261, 1332
AcoI	YGGCCR	1 cut(s)	583
AcsI	RAATTY	3 cut(s)	597, 812, 980
AcuI	CTGAAG	1 cut(s)	1062
AcyI	GRCGYC	6 cut(s)	129, 287, 458, 497, 795, 920
AfaI	GTAC	6 cut(s)	25, 482, 632, 656, 830, 1299
AfiI	CCNNNNNNNGG	3 cut(s)	224, 310, 1354
AflIII	ACRYGT	1 cut(s)	181
AgeI	ACCGGT	1 cut(s)	956
AgsI	TTSAA	7 cut(s)	379, 539, 652, 691, 1126, 1286, 1418
AhlI	ACTAGT	1 cut(s)	1294
AjnI	CCWGG	2 cut(s)	223, 1025
AjuI	GAANNNNNNNTTGG	2 cut(s)	170, 202
AluBI	AGCT	4 cut(s)	33, 242, 409, 1250
AluI	AGCT	4 cut(s)	33, 242, 409, 1250
Alw21I	GWGCWC	2 cut(s)	489, 1092
Alw26I	GTCTC	4 cut(s)	329, 353, 792, 802
Alw44I	GTGCAC	1 cut(s)	485
AlwI	GGATC	2 cut(s)	1261, 1332
AlwNI	CAGNNNCTG	1 cut(s)	1109
Ama87I	CYCGRG	3 cut(s)	92, 1226, 1437
AoxI	GGCC	7 cut(s)	226, 279, 583, 805, 1008, 1055, 1064
ApaLI	GTGCAC	1 cut(s)	485
ApeKI	GCWGC	7 cut(s)	18, 33, 466, 544, 1103, 1307, 1310
ApoI	RAATTY	3 cut(s)	597, 812, 980
ArsI	GACNNNNNNTTYG	2 cut(s)	425, 457
AsiGI	ACCGGT	1 cut(s)	956
Asp700I	GAANNNNTTC	2 cut(s)	374, 1121
AspLEI	GCGC	1 cut(s)	1478
AspS9I	GGNCC	5 cut(s)	61, 805, 959, 1055, 1064
AsuC2I	CCSGG	3 cut(s)	514, 1438, 1439
AsuHPI	GGTGA	5 cut(s)	124, 376, 424, 773, 946
AvaI	CYCGRG	3 cut(s)	92, 1226, 1437
AvaII	GGWCC	2 cut(s)	61, 959
BaeGI	GKGCMC	1 cut(s)	489
BalI	TGGCCA	1 cut(s)	585
BarI	GAAGNNNNNNTAC	2 cut(s)	1281, 1313
BbsI	GAAGAC	3 cut(s)	178, 250, 560
Bbv12I	GWGCWC	2 cut(s)	489, 1092
BbvI	GCAGC	7 cut(s)	20, 30, 478, 531, 1115, 1294, 1322
BccI	CCATC	3 cut(s)	47, 242, 678
BceAI	ACGGC	1 cut(s)	142
BciT130I	CCWGG	2 cut(s)	225, 1027
BclI	TGATCA	2 cut(s)	778, 907
BcnI	CCSGG	3 cut(s)	514, 1438, 1439
BcoDI	GTCTC	4 cut(s)	329, 353, 792, 802
BcuI	ACTAGT	1 cut(s)	1294
BfaI	CTAG	3 cut(s)	939, 1188, 1295
BfmI	CTRYAG	1 cut(s)	1308
BglI	GCCNNNNNGGC	2 cut(s)	475, 1063
BisI	GCNGC	8 cut(s)	19, 34, 467, 545, 1104, 1308, 1311, 1493
BlsI	GCNGC	8 cut(s)	20, 35, 468, 546, 1105, 1309, 1312, 1494
Bme1390I	CCNGG	5 cut(s)	225, 514, 1027, 1438, 1439
Bme18I	GGWCC	2 cut(s)	61, 959
BmeT110I	CYCGRG	3 cut(s)	92, 1226, 1437
BmgT120I	GGNCC	5 cut(s)	61, 805, 959, 1055, 1064
BmiI	GGNNCC	3 cut(s)	1005, 1065, 1168
BmrFI	CCNGG	5 cut(s)	225, 514, 1027, 1438, 1439
BmrI	ACTGGG	1 cut(s)	1208
BmsI	GCATC	2 cut(s)	1144, 1255
BmuI	ACTGGG	1 cut(s)	1208
BpiI	GAAGAC	3 cut(s)	178, 250, 560
BplI	GAGNNNNNCTC	4 cut(s)	59, 91, 1079, 1111
BpuMI	CCSGG	3 cut(s)	514, 1438, 1439
Bsa29I	ATCGAT	1 cut(s)	660
BsaAI	YACGTR	1 cut(s)	484
BsaBI	GATNNNNATC	1 cut(s)	1341
BsaHI	GRCGYC	6 cut(s)	129, 287, 458, 497, 795, 920
BsaJI	CCNNGG	6 cut(s)	223, 269, 282, 570, 1025, 1437
BsaWI	WCCGGW	1 cut(s)	956
BsaXI	ACNNNNNCTCC	2 cut(s)	158, 188
Bsc4I	CCNNNNNNNGG	3 cut(s)	224, 310, 1354
Bse118I	RCCGGY	3 cut(s)	454, 474, 956
Bse1I	ACTGG	3 cut(s)	395, 1214, 1359
Bse8I	GATNNNNATC	1 cut(s)	1341
BseBI	CCWGG	2 cut(s)	225, 1027
BseCI	ATCGAT	1 cut(s)	660
BseDI	CCNNGG	6 cut(s)	223, 269, 282, 570, 1025, 1437
BseGI	GGATG	2 cut(s)	105, 234
BseJI	GATNNNNATC	1 cut(s)	1341
BseLI	CCNNNNNNNGG	3 cut(s)	224, 310, 1354
BseMII	CTCAG	3 cut(s)	348, 813, 866
BseNI	ACTGG	3 cut(s)	395, 1214, 1359
BseRI	GAGGAG	3 cut(s)	590, 719, 1084
BseSI	GKGCMC	1 cut(s)	489
BseXI	GCAGC	7 cut(s)	20, 30, 478, 531, 1115, 1294, 1322
BsgI	GTGCAG	1 cut(s)	948
Bsh1285I	CGRYCG	1 cut(s)	988
BshFI	GGCC	7 cut(s)	228, 281, 585, 807, 1010, 1057, 1066
BshTI	ACCGGT	1 cut(s)	956
BshVI	ATCGAT	1 cut(s)	660
BsiEI	CGRYCG	1 cut(s)	988
BsiHKAI	GWGCWC	2 cut(s)	489, 1092
BsiHKCI	CYCGRG	3 cut(s)	92, 1226, 1437
BsiSI	CCGG	7 cut(s)	383, 455, 475, 513, 957, 1007, 1438
BslFI	GGGAC	3 cut(s)	91, 529, 745
BslI	CCNNNNNNNGG	3 cut(s)	224, 310, 1354
BsmAI	GTCTC	4 cut(s)	329, 353, 792, 802
BsmBI	CGTCTC	3 cut(s)	353, 792, 802
BsmFI	GGGAC	3 cut(s)	91, 529, 745
BsnI	GGCC	7 cut(s)	228, 281, 585, 807, 1010, 1057, 1066
BsoBI	CYCGRG	3 cut(s)	92, 1226, 1437
Bsp1286I	GDGCHC	2 cut(s)	489, 1092
Bsp1407I	TGTACA	1 cut(s)	630
Bsp143I	GATC	9 cut(s)	679, 751, 778, 842, 907, 985, 1253, 1272, 1324
Bsp19I	CCATGG	2 cut(s)	269, 570
BspACI	CCGC	5 cut(s)	73, 204, 347, 897, 1493
BspANI	GGCC	7 cut(s)	228, 281, 585, 807, 1010, 1057, 1066
BspCNI	CTCAG	3 cut(s)	349, 812, 865
BspDI	ATCGAT	1 cut(s)	660
BspHI	TCATGA	1 cut(s)	790
BspLI	GGNNCC	3 cut(s)	1005, 1065, 1168
BspMAI	CTGCAG	1 cut(s)	1312
BspPI	GGATC	2 cut(s)	1261, 1332
BsrBI	CCGCTC	1 cut(s)	75
BsrFI	RCCGGY	3 cut(s)	454, 474, 956
BsrGI	TGTACA	1 cut(s)	630
BsrI	ACTGG	3 cut(s)	395, 1214, 1359
BssAI	RCCGGY	3 cut(s)	454, 474, 956
BssECI	CCNNGG	6 cut(s)	223, 269, 282, 570, 1025, 1437
BssMI	GATC	9 cut(s)	679, 751, 778, 842, 907, 985, 1253, 1272, 1324
BssNAI	GTATAC	1 cut(s)	883
BssNI	GRCGYC	6 cut(s)	129, 287, 458, 497, 795, 920
BssT1I	CCWWGG	2 cut(s)	269, 570
Bst1107I	GTATAC	1 cut(s)	883
Bst2UI	CCWGG	2 cut(s)	225, 1027
Bst4CI	ACNGT	5 cut(s)	49, 364, 730, 1045, 1111
BstACI	GRCGYC	6 cut(s)	129, 287, 458, 497, 795, 920
BstAPI	GCANNNNNTGC	1 cut(s)	484
BstAUI	TGTACA	1 cut(s)	630
BstBAI	YACGTR	1 cut(s)	484
BstC8I	GCNNGC	4 cut(s)	266, 345, 456, 583
BstDEI	CTNAG	4 cut(s)	357, 641, 799, 852
BstDSI	CCRYGG	2 cut(s)	269, 570
BstENI	CCTNNNNNAGG	1 cut(s)	222
BstF5I	GGATG	2 cut(s)	105, 234
BstHHI	GCGC	1 cut(s)	1478
BstKTI	GATC	9 cut(s)	682, 754, 781, 845, 910, 988, 1256, 1275, 1327
BstMAI	GTCTC	4 cut(s)	329, 353, 792, 802
BstMBI	GATC	9 cut(s)	679, 751, 778, 842, 907, 985, 1253, 1272, 1324
BstMCI	CGRYCG	1 cut(s)	988
BstMWI	GCNNNNNNNGC	6 cut(s)	329, 353, 475, 484, 708, 1063
BstNI	CCWGG	2 cut(s)	225, 1027
BstNSI	RCATGY	1 cut(s)	91
BstSCI	CCNGG	5 cut(s)	223, 512, 1025, 1436, 1437
BstSFI	CTRYAG	1 cut(s)	1308
BstSLI	GKGCMC	1 cut(s)	489
BstV1I	GCAGC	7 cut(s)	20, 30, 478, 531, 1115, 1294, 1322
BstV2I	GAAGAC	3 cut(s)	178, 250, 560
BstX2I	RGATCY	1 cut(s)	1253
BstXI	CCANNNNNNTGG	1 cut(s)	593
BstYI	RGATCY	1 cut(s)	1253
BstZ17I	GTATAC	1 cut(s)	883
Bsu15I	ATCGAT	1 cut(s)	660
BsuRI	GGCC	7 cut(s)	228, 281, 585, 807, 1010, 1057, 1066
BsuTUI	ATCGAT	1 cut(s)	660
BtgI	CCRYGG	2 cut(s)	269, 570
BtsCI	GGATG	2 cut(s)	105, 234
BtsI	GCAGTG	2 cut(s)	432, 697
BtsIMutI	CAGTG	5 cut(s)	402, 432, 697, 860, 1050
Cac8I	GCNNGC	4 cut(s)	266, 345, 456, 583
CaiI	CAGNNNCTG	1 cut(s)	1109
CciI	TCATGA	1 cut(s)	790
CfoI	GCGC	1 cut(s)	1478
Cfr10I	RCCGGY	3 cut(s)	454, 474, 956
Cfr13I	GGNCC	5 cut(s)	61, 805, 959, 1055, 1064
Cfr9I	CCCGGG	1 cut(s)	1437
ClaI	ATCGAT	1 cut(s)	660
CseI	GACGC	5 cut(s)	118, 276, 447, 472, 928
Csp6I	GTAC	6 cut(s)	24, 481, 631, 655, 829, 1298
CspAI	ACCGGT	1 cut(s)	956
CviAII	CATG	8 cut(s)	88, 270, 503, 571, 791, 893, 911, 1052
CviQI	GTAC	6 cut(s)	24, 481, 631, 655, 829, 1298
DdeI	CTNAG	4 cut(s)	357, 641, 799, 852
DpnI	GATC	9 cut(s)	681, 753, 780, 844, 909, 987, 1255, 1274, 1326
DpnII	GATC	9 cut(s)	679, 751, 778, 842, 907, 985, 1253, 1272, 1324
DraI	TTTAAA	2 cut(s)	979, 1408
EaeI	YGGCCR	1 cut(s)	583
EciI	GGCGGA	2 cut(s)	193, 912
Eco130I	CCWWGG	2 cut(s)	269, 570
Eco147I	AGGCCT	1 cut(s)	281
Eco47I	GGWCC	2 cut(s)	61, 959
Eco57I	CTGAAG	1 cut(s)	1062
Eco88I	CYCGRG	3 cut(s)	92, 1226, 1437
EcoNI	CCTNNNNNAGG	1 cut(s)	222
EcoRII	CCWGG	2 cut(s)	223, 1025
EcoT14I	CCWWGG	2 cut(s)	269, 570
ErhI	CCWWGG	2 cut(s)	269, 570
Esp3I	CGTCTC	3 cut(s)	353, 792, 802
FaeI	CATG	8 cut(s)	91, 273, 506, 574, 794, 896, 914, 1055
FaqI	GGGAC	3 cut(s)	91, 529, 745
FatI	CATG	8 cut(s)	87, 269, 502, 570, 790, 892, 910, 1051
FauI	CCCGC	1 cut(s)	340
FbaI	TGATCA	2 cut(s)	778, 907
FblI	GTMKAC	4 cut(s)	213, 246, 461, 882
Fnu4HI	GCNGC	8 cut(s)	19, 34, 467, 545, 1104, 1308, 1311, 1493
FokI	GGATG	2 cut(s)	112, 221
Fsp4HI	GCNGC	8 cut(s)	19, 34, 467, 545, 1104, 1308, 1311, 1493
FspBI	CTAG	3 cut(s)	939, 1188, 1295
FspI	TGCGCA	1 cut(s)	1477
GlaI	GCGC	1 cut(s)	1477
GluI	GCNGC	8 cut(s)	19, 34, 467, 545, 1104, 1308, 1311, 1493
HaeIII	GGCC	7 cut(s)	228, 281, 585, 807, 1010, 1057, 1066
HapII	CCGG	7 cut(s)	383, 455, 475, 513, 957, 1007, 1438
HgaI	GACGC	5 cut(s)	118, 276, 447, 472, 928
HhaI	GCGC	1 cut(s)	1478
Hin1I	GRCGYC	6 cut(s)	129, 287, 458, 497, 795, 920
Hin1II	CATG	8 cut(s)	91, 273, 506, 574, 794, 896, 914, 1055
Hin6I	GCGC	1 cut(s)	1476
HinP1I	GCGC	1 cut(s)	1476
HincII	GTYRAC	2 cut(s)	214, 462
HindII	GTYRAC	2 cut(s)	214, 462
HinfI	GANTC	9 cut(s)	11, 137, 143, 318, 566, 637, 744, 900, 1457
HpaII	CCGG	7 cut(s)	383, 455, 475, 513, 957, 1007, 1438
HphI	GGTGA	5 cut(s)	124, 376, 424, 773, 946
Hpy166II	GTNNAC	6 cut(s)	214, 247, 462, 487, 829, 883
Hpy188I	TCNGA	9 cut(s)	10, 142, 166, 277, 609, 802, 918, 1081, 1378
Hpy188III	TCNNGA	5 cut(s)	691, 791, 944, 1286, 1418
Hpy8I	GTNNAC	6 cut(s)	214, 247, 462, 487, 829, 883
Hpy99I	CGWCG	7 cut(s)	215, 463, 466, 499, 502, 890, 922
HpyAV	CCTTC	7 cut(s)	88, 533, 1021, 1068, 1083, 1327, 1391
HpyCH4III	ACNGT	5 cut(s)	49, 364, 730, 1045, 1111
HpyCH4IV	ACGT	5 cut(s)	181, 210, 483, 497, 795
HpyCH4V	TGCA	7 cut(s)	425, 487, 965, 1051, 1103, 1268, 1310
HpyF10VI	GCNNNNNNNGC	6 cut(s)	329, 353, 475, 484, 708, 1063
HpyF3I	CTNAG	4 cut(s)	357, 641, 799, 852
HpySE526I	ACGT	5 cut(s)	181, 210, 483, 497, 795
Hsp92I	GRCGYC	6 cut(s)	129, 287, 458, 497, 795, 920
Hsp92II	CATG	8 cut(s)	91, 273, 506, 574, 794, 896, 914, 1055
HspAI	GCGC	1 cut(s)	1476
KroI	GCCGGC	1 cut(s)	454
KroNI	GCCGGC	1 cut(s)	456
Ksp22I	TGATCA	2 cut(s)	778, 907
Kzo9I	GATC	9 cut(s)	679, 751, 778, 842, 907, 985, 1253, 1272, 1324
LmnI	GCTCC	3 cut(s)	80, 406, 1400
Lsp1109I	GCAGC	7 cut(s)	20, 30, 478, 531, 1115, 1294, 1322
LweI	GCATC	2 cut(s)	1144, 1255
MaeI	CTAG	3 cut(s)	939, 1188, 1295
MaeII	ACGT	5 cut(s)	181, 210, 483, 497, 795
MaeIII	GTNAC	3 cut(s)	130, 431, 1216
MalI	GATC	9 cut(s)	681, 753, 780, 844, 909, 987, 1255, 1274, 1326
MbiI	CCGCTC	1 cut(s)	75
MboI	GATC	9 cut(s)	679, 751, 778, 842, 907, 985, 1253, 1272, 1324
MboII	GAAGA	8 cut(s)	178, 181, 250, 565, 739, 1068, 1129, 1301
MfeI	CAATTG	2 cut(s)	1098, 1301
MflI	RGATCY	1 cut(s)	1253
MhlI	GDGCHC	2 cut(s)	489, 1092
MlsI	TGGCCA	1 cut(s)	585
MluCI	AATT	9 cut(s)	597, 713, 812, 876, 980, 1017, 1098, 1301, 1464
MluNI	TGGCCA	1 cut(s)	585
MlyI	GAGTC	3 cut(s)	20, 152, 575
MmeI	TCCRAC	3 cut(s)	189, 255, 941
Mox20I	TGGCCA	1 cut(s)	585
MroNI	GCCGGC	1 cut(s)	454
MroXI	GAANNNNTTC	2 cut(s)	374, 1121
MscI	TGGCCA	1 cut(s)	585
MseI	TTAA	4 cut(s)	927, 978, 1113, 1407
MslI	CAYNNNNRTG	1 cut(s)	395
Msp20I	TGGCCA	1 cut(s)	585
MspI	CCGG	7 cut(s)	383, 455, 475, 513, 957, 1007, 1438
MspR9I	CCNGG	5 cut(s)	225, 514, 1027, 1438, 1439
MunI	CAATTG	2 cut(s)	1098, 1301
MvaI	CCWGG	2 cut(s)	225, 1027
MwoI	GCNNNNNNNGC	6 cut(s)	329, 353, 475, 484, 708, 1063
NaeI	GCCGGC	1 cut(s)	456
NciI	CCSGG	3 cut(s)	514, 1438, 1439
NcoI	CCATGG	2 cut(s)	269, 570
NdeII	GATC	9 cut(s)	679, 751, 778, 842, 907, 985, 1253, 1272, 1324
NgoMIV	GCCGGC	1 cut(s)	454
NlaIII	CATG	8 cut(s)	91, 273, 506, 574, 794, 896, 914, 1055
NlaIV	GGNNCC	3 cut(s)	1005, 1065, 1168
NmeAIII	GCCGAG	3 cut(s)	263, 358, 470
NmuCI	GTSAC	2 cut(s)	130, 431
NsbI	TGCGCA	1 cut(s)	1477
NspI	RCATGY	1 cut(s)	91
PagI	TCATGA	1 cut(s)	790
PceI	AGGCCT	1 cut(s)	281
PcsI	WCGNNNNNNNCGW	2 cut(s)	132, 467
PdiI	GCCGGC	1 cut(s)	456
PdmI	GAANNNNTTC	2 cut(s)	374, 1121
PfeI	GAWTC	6 cut(s)	137, 318, 637, 744, 900, 1457
PflMI	CCANNNNNTGG	1 cut(s)	1354
PfoI	TCCNGGA	1 cut(s)	512
PinAI	ACCGGT	1 cut(s)	956
PkrI	GCNGC	8 cut(s)	20, 35, 468, 546, 1105, 1309, 1312, 1494
Ple19I	CGATCG	1 cut(s)	988
PleI	GAGTC	3 cut(s)	19, 151, 574
PpsI	GAGTC	3 cut(s)	19, 151, 574
Ppu21I	YACGTR	1 cut(s)	484
Psp6I	CCWGG	2 cut(s)	223, 1025
PspGI	CCWGG	2 cut(s)	223, 1025
PspN4I	GGNNCC	3 cut(s)	1005, 1065, 1168
PspPI	GGNCC	5 cut(s)	61, 805, 959, 1055, 1064
PsrI	GAACNNNNNNTAC	2 cut(s)	464, 496
PstI	CTGCAG	1 cut(s)	1312
PstNI	CAGNNNCTG	1 cut(s)	1109
PsuI	RGATCY	1 cut(s)	1253
PvuI	CGATCG	1 cut(s)	988
RsaI	GTAC	6 cut(s)	25, 482, 632, 656, 830, 1299
RsaNI	GTAC	6 cut(s)	24, 481, 631, 655, 829, 1298
RseI	CAYNNNNRTG	1 cut(s)	395
SalI	GTCGAC	2 cut(s)	212, 460
SaqAI	TTAA	4 cut(s)	927, 978, 1113, 1407
SatI	GCNGC	8 cut(s)	19, 34, 467, 545, 1104, 1308, 1311, 1493
Sau3AI	GATC	9 cut(s)	679, 751, 778, 842, 907, 985, 1253, 1272, 1324
Sau96I	GGNCC	5 cut(s)	61, 805, 959, 1055, 1064
SchI	GAGTC	3 cut(s)	20, 152, 575
ScrFI	CCNGG	5 cut(s)	225, 514, 1027, 1438, 1439
SduI	GDGCHC	2 cut(s)	489, 1092
SfaNI	GCATC	2 cut(s)	1144, 1255
SfcI	CTRYAG	1 cut(s)	1308
SfiI	GGCCNNNNNGGCC	1 cut(s)	1063
SgrDI	CGTCGACG	1 cut(s)	460
SinI	GGWCC	2 cut(s)	61, 959
SmaI	CCCGGG	1 cut(s)	1439
SmiMI	CAYNNNNRTG	1 cut(s)	395
SpeI	ACTAGT	1 cut(s)	1294
Sse9I	AATT	9 cut(s)	597, 713, 812, 876, 980, 1017, 1098, 1301, 1464
SseBI	AGGCCT	1 cut(s)	281
SsiI	CCGC	5 cut(s)	73, 204, 347, 897, 1493
SspMI	CTAG	3 cut(s)	939, 1188, 1295
StuI	AGGCCT	1 cut(s)	281
StyD4I	CCNGG	5 cut(s)	223, 512, 1025, 1436, 1437
StyI	CCWWGG	2 cut(s)	269, 570
TaaI	ACNGT	5 cut(s)	49, 364, 730, 1045, 1111
TaiI	ACGT	5 cut(s)	184, 213, 486, 500, 798
TaqI	TCGA	7 cut(s)	14, 81, 213, 461, 660, 750, 984
TasI	AATT	9 cut(s)	597, 713, 812, 876, 980, 1017, 1098, 1301, 1464
TatI	WGTACW	4 cut(s)	23, 630, 654, 1297
TauI	GCSGC	1 cut(s)	1495
TfiI	GAWTC	6 cut(s)	137, 318, 637, 744, 900, 1457
Tru1I	TTAA	4 cut(s)	927, 978, 1113, 1407
Tru9I	TTAA	4 cut(s)	927, 978, 1113, 1407
TscAI	CASTG	5 cut(s)	402, 432, 704, 860, 1050
TseFI	GTSAC	2 cut(s)	130, 431
TseI	GCWGC	7 cut(s)	18, 33, 466, 544, 1103, 1307, 1310
Tsp45I	GTSAC	2 cut(s)	130, 431
TspDTI	ATGAA	6 cut(s)	116, 383, 862, 876, 1437, 1505
TspMI	CCCGGG	1 cut(s)	1437
TspRI	CASTG	5 cut(s)	402, 432, 704, 860, 1050
Van91I	CCANNNNNTGG	1 cut(s)	1354
VneI	GTGCAC	1 cut(s)	485
VpaK11BI	GGWCC	2 cut(s)	61, 959
XagI	CCTNNNNNAGG	1 cut(s)	222
XapI	RAATTY	3 cut(s)	597, 812, 980
XceI	RCATGY	1 cut(s)	91
XcmI	CCANNNNNNNNNTGG	1 cut(s)	46
XmaI	CCCGGG	1 cut(s)	1437
XmiI	GTMKAC	4 cut(s)	213, 246, 461, 882
XmnI	GAANNNNTTC	2 cut(s)	374, 1121
XspI	CTAG	3 cut(s)	939, 1188, 1295
ZraI	GACGTC	2 cut(s)	498, 796

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.