Rroxscaffold_4G00325580

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0025751
Physical Location & Seq
Forward (+)
57309058 .. 57311037
1980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325580.1

Sequence Viewer

Length: 330 bp
ATGGATTTACTTGTTGCTGTGAAGATCCTCAACAATTCAAATGAAAAGGGGGAAGATTTTATAAATGAAGTGGGAACAATGGGTCGAATTCACCATATAAATGTGGTTCGTATGGTTGGCTTAAATGTCGATGGATATATTCAAGCTCTTGTTTACGAGTTCTTACCAAATGGTTCTGTAATACCCCGAAAATTTAAATTATTTTCTGAGAACATTTTGGAAATGATTTCGTGGCCATGGGCGAAAGTACGAAACTTGGAGGAGTGGTGGAATGAGTTCGAACGAATTTATTTTCAAATCGAACGTTTTTTAGGGGGCCTCGGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

109

Amino Acids

12.79

Weight (kDa)

5.67

Isoelectric Point (pI)

20.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 4 - 60 7.5e-08 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025751)

Species Orthologous Gene IDs
rosa_roxburghii Rroxscaffold_159G00432750 Rroxscaffold_4G00325570 Rroxscaffold_4G00325580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 62
AclI AACGTT 1 cut(s) 304
AclWI GGATC 1 cut(s) 19
AcoI YGGCCR 1 cut(s) 233
AcsI RAATTY 3 cut(s) 87, 191, 285
AfaI GTAC 1 cut(s) 249
AgsI TTSAA 3 cut(s) 39, 143, 296
AluBI AGCT 1 cut(s) 146
AluI AGCT 1 cut(s) 146
AlwI GGATC 1 cut(s) 19
AoxI GGCC 2 cut(s) 233, 316
ApoI RAATTY 3 cut(s) 87, 191, 285
Asp700I GAANNNNTTC 1 cut(s) 275
AspS9I GGNCC 1 cut(s) 316
AsuHPI GGTGA 1 cut(s) 83
AsuII TTCGAA 1 cut(s) 279
BalI TGGCCA 1 cut(s) 235
BccI CCATC 1 cut(s) 125
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 1 cut(s) 317
Bpu14I TTCGAA 1 cut(s) 279
BsaJI CCNNGG 2 cut(s) 236, 319
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
BseDI CCNNGG 2 cut(s) 236, 319
BseMII CTCAG 1 cut(s) 198
BseRI GAGGAG 1 cut(s) 275
BshFI GGCC 2 cut(s) 235, 318
BsnI GGCC 2 cut(s) 235, 318
Bsp119I TTCGAA 1 cut(s) 279
Bsp143I GATC 1 cut(s) 24
Bsp19I CCATGG 1 cut(s) 236
BspANI GGCC 2 cut(s) 235, 318
BspCNI CTCAG 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 317
BspPI GGATC 1 cut(s) 19
BspT104I TTCGAA 1 cut(s) 279
BssECI CCNNGG 2 cut(s) 236, 319
BssMI GATC 1 cut(s) 24
BssT1I CCWWGG 1 cut(s) 236
BstBI TTCGAA 1 cut(s) 279
BstDEI CTNAG 1 cut(s) 207
BstDSI CCRYGG 1 cut(s) 236
BstKTI GATC 1 cut(s) 27
BstMBI GATC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 24
BstYI RGATCY 1 cut(s) 24
BsuRI GGCC 2 cut(s) 235, 318
BtgI CCRYGG 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 316
Csp6I GTAC 1 cut(s) 248
CviAII CATG 1 cut(s) 237
CviJI RGCY 4 cut(s) 120, 146, 235, 318
CviKI_1 RGCY 4 cut(s) 120, 146, 235, 318
CviQI GTAC 1 cut(s) 248
DdeI CTNAG 1 cut(s) 207
DpnI GATC 1 cut(s) 26
DpnII GATC 1 cut(s) 24
DraI TTTAAA 1 cut(s) 196
EaeI YGGCCR 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 236
EcoO109I RGGNCCY 1 cut(s) 316
EcoRI GAATTC 1 cut(s) 87
EcoT14I CCWWGG 1 cut(s) 236
ErhI CCWWGG 1 cut(s) 236
FaeI CATG 1 cut(s) 240
FaiI YATR 6 cut(s) 62, 96, 98, 113, 138, 238
FatI CATG 1 cut(s) 236
HaeIII GGCC 2 cut(s) 235, 318
Hin1II CATG 1 cut(s) 240
HphI GGTGA 1 cut(s) 83
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 2 cut(s) 208, 323
Hpy8I GTNNAC 1 cut(s) 154
HpyCH4IV ACGT 1 cut(s) 304
HpyF3I CTNAG 1 cut(s) 207
HpySE526I ACGT 1 cut(s) 304
Hsp92II CATG 1 cut(s) 240
Kzo9I GATC 1 cut(s) 24
MaeII ACGT 1 cut(s) 304
MalI GATC 1 cut(s) 26
MboI GATC 1 cut(s) 24
MboII GAAGA 2 cut(s) 34, 65
MflI RGATCY 1 cut(s) 24
MlsI TGGCCA 1 cut(s) 235
MluCI AATT 5 cut(s) 34, 87, 191, 197, 285
MluNI TGGCCA 1 cut(s) 235
MnlI CCTC 3 cut(s) 38, 253, 329
Mox20I TGGCCA 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 275
MscI TGGCCA 1 cut(s) 235
MseI TTAA 2 cut(s) 122, 195
MslI CAYNNNNRTG 1 cut(s) 99
Msp20I TGGCCA 1 cut(s) 235
NcoI CCATGG 1 cut(s) 236
NdeII GATC 1 cut(s) 24
NlaIII CATG 1 cut(s) 240
NlaIV GGNNCC 1 cut(s) 317
NspV TTCGAA 1 cut(s) 279
PdmI GAANNNNTTC 1 cut(s) 275
PsiI TTATAA 1 cut(s) 62
Psp1406I AACGTT 1 cut(s) 304
PspN4I GGNNCC 1 cut(s) 317
PspPI GGNCC 1 cut(s) 316
PsuI RGATCY 1 cut(s) 24
RsaI GTAC 1 cut(s) 249
RsaNI GTAC 1 cut(s) 248
RseI CAYNNNNRTG 1 cut(s) 99
SaqAI TTAA 2 cut(s) 122, 195
Sau3AI GATC 1 cut(s) 24
Sau96I GGNCC 1 cut(s) 316
SetI ASST 2 cut(s) 148, 307
SfuI TTCGAA 1 cut(s) 279
SgeI CNNG 8 cut(s) 23, 155, 161, 169, 198, 243, 249, 268
SmiI ATTTAAAT 1 cut(s) 196
SmiMI CAYNNNNRTG 1 cut(s) 99
Sse9I AATT 5 cut(s) 34, 87, 191, 197, 285
StyI CCWWGG 1 cut(s) 236
SwaI ATTTAAAT 1 cut(s) 196
TaiI ACGT 1 cut(s) 307
TaqI TCGA 4 cut(s) 85, 129, 279, 300
TasI AATT 5 cut(s) 34, 87, 191, 197, 285
Tru1I TTAA 2 cut(s) 122, 195
Tru9I TTAA 2 cut(s) 122, 195
TspDTI ATGAA 2 cut(s) 57, 81
XapI RAATTY 3 cut(s) 87, 191, 285
XmnI GAANNNNTTC 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.