Rroxscaffold_5G00334240

RING-like zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0019308
Physical Location & Seq
Forward (+)
1575971 .. 1576528
558 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_5G00334240.1

Sequence Viewer

Length: 438 bp
ATGGCTGGGTTTGGAAGGCTACCTGGTGTTGGTCTGCCTCCAAGAAGAAGATCAAACCACACCCACCACCGTCAAGAAGGAAATCAATGGTTCAAGCCATCAATGACTTCCATGGATATTCTGGATGATGAAGCAGCTCATAAAGCCCGGGAAAGACTAGAGAAAAGGCTTGGCCGTTTCAGACCCTGCTCAAGCAACCATTATGCCAAAATGGGTTCAAGTTCAAAGCAATTAGGGAGGCCTTCACGGGGATCCTTCTTCTCTCTCAGCAGAAATGAAATAGATGTTTGTGCTATTTGCTTGGAGAATTTTGAGGCAGGAGAAGAGGCCATGGAGCTCGCGTGTGCCCATAAATTTCATTCCCACTGTCTTTCGTCTTGGCTTCATGCCCATTCGGATTGCCCCTATTGTCGGACCAATGTTTCTGTTTGTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

145

Amino Acids

16.37

Weight (kDa)

8.88

Isoelectric Point (pI)

59.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 96 - 138 2.4e-14 Ring finger domain
zf-RING_11 PF17123 97 - 124 6.3e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 97 - 137 9.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 97 - 137 1.3e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019308)

Species Orthologous Gene IDs
prunus_persica Prupe.1G020100_v2.0.a1 Prupe.1G020100_v2.0.a1
pyrus_communis pycom13g17220
rosa_roxburghii Rroxscaffold_5G00334240
rosa_rugosa Rorug03G0310700
rosa_samantha Rh4AG016800 Rh4BG011600 Rh4DG012500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 341
AclWI GGATC 2 cut(s) 246, 259
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 2 cut(s) 307, 353
AfiI CCNNNNNNNGG 3 cut(s) 29, 248, 411
AgsI TTSAA 3 cut(s) 94, 219, 225
AjnI CCWGG 1 cut(s) 22
AluBI AGCT 2 cut(s) 137, 337
AluI AGCT 2 cut(s) 137, 337
Alw21I GWGCWC 1 cut(s) 339
AlwI GGATC 2 cut(s) 246, 259
AlwNI CAGNNNCTG 1 cut(s) 186
Ama87I CYCGRG 1 cut(s) 147
AoxI GGCC 3 cut(s) 172, 239, 327
ApeKI GCWGC 1 cut(s) 134
ApoI RAATTY 2 cut(s) 307, 353
AspS9I GGNCC 1 cut(s) 414
AsuC2I CCSGG 2 cut(s) 148, 149
AvaI CYCGRG 1 cut(s) 147
AvaII GGWCC 1 cut(s) 414
BaeGI GKGCMC 1 cut(s) 349
BamHI GGATCC 1 cut(s) 251
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 1 cut(s) 146
BccI CCATC 1 cut(s) 106
BceAI ACGGC 1 cut(s) 159
BciT130I CCWGG 1 cut(s) 24
BcnI CCSGG 2 cut(s) 148, 149
BfaI CTAG 1 cut(s) 158
BisI GCNGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 136
Bme1390I CCNGG 3 cut(s) 24, 148, 149
Bme18I GGWCC 1 cut(s) 414
BmeT110I CYCGRG 1 cut(s) 147
BmgT120I GGNCC 1 cut(s) 414
BmiI GGNNCC 1 cut(s) 253
BmrFI CCNGG 3 cut(s) 24, 148, 149
BpuEI CTTGAG 1 cut(s) 175
BpuMI CCSGG 2 cut(s) 148, 149
BsaJI CCNNGG 3 cut(s) 111, 147, 330
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 248, 411
BseBI CCWGG 1 cut(s) 24
BseDI CCNNGG 3 cut(s) 111, 147, 330
BseGI GGATG 1 cut(s) 130
BseLI CCNNNNNNNGG 3 cut(s) 29, 248, 411
BseMII CTCAG 1 cut(s) 280
BseSI GKGCMC 1 cut(s) 349
BseXI GCAGC 1 cut(s) 146
BseYI CCCAGC 1 cut(s) 5
Bsh1236I CGCG 1 cut(s) 341
BshFI GGCC 3 cut(s) 174, 241, 329
BsiHKAI GWGCWC 1 cut(s) 339
BsiHKCI CYCGRG 1 cut(s) 147
BsiSI CCGG 1 cut(s) 148
BslI CCNNNNNNNGG 3 cut(s) 29, 248, 411
BsnI GGCC 3 cut(s) 174, 241, 329
BsoBI CYCGRG 1 cut(s) 147
Bsp1286I GDGCHC 2 cut(s) 339, 349
Bsp143I GATC 2 cut(s) 50, 251
Bsp19I CCATGG 2 cut(s) 111, 330
BspANI GGCC 3 cut(s) 174, 241, 329
BspCNI CTCAG 1 cut(s) 279
BspFNI CGCG 1 cut(s) 341
BspLI GGNNCC 1 cut(s) 253
BspPI GGATC 2 cut(s) 246, 259
BssECI CCNNGG 3 cut(s) 111, 147, 330
BssMI GATC 2 cut(s) 50, 251
BssT1I CCWWGG 2 cut(s) 111, 330
Bst2UI CCWGG 1 cut(s) 24
Bst4CI ACNGT 2 cut(s) 71, 368
Bst6I CTCTTC 1 cut(s) 318
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 1 cut(s) 266
BstDSI CCRYGG 2 cut(s) 111, 330
BstF5I GGATG 1 cut(s) 130
BstFNI CGCG 1 cut(s) 341
BstKTI GATC 2 cut(s) 53, 254
BstMBI GATC 2 cut(s) 50, 251
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstNI CCWGG 1 cut(s) 24
BstSCI CCNGG 3 cut(s) 22, 146, 147
BstSLI GKGCMC 1 cut(s) 349
BstUI CGCG 1 cut(s) 341
BstV1I GCAGC 1 cut(s) 146
BstX2I RGATCY 1 cut(s) 251
BstYI RGATCY 1 cut(s) 251
BsuRI GGCC 3 cut(s) 174, 241, 329
BtgI CCRYGG 2 cut(s) 111, 330
BtsCI GGATG 1 cut(s) 130
BtsIMutI CAGTG 1 cut(s) 364
Cac8I GCNNGC 1 cut(s) 339
CaiI CAGNNNCTG 1 cut(s) 186
Cfr13I GGNCC 1 cut(s) 414
Cfr9I CCCGGG 1 cut(s) 147
CsiI ACCWGGT 1 cut(s) 22
CviAII CATG 3 cut(s) 112, 331, 386
DdeI CTNAG 1 cut(s) 266
DpnI GATC 2 cut(s) 52, 253
DpnII GATC 2 cut(s) 50, 251
EaeI YGGCCR 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 2 cut(s) 111, 330
Eco147I AGGCCT 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 339
Eco47I GGWCC 1 cut(s) 414
Eco53kI GAGCTC 1 cut(s) 337
Eco88I CYCGRG 1 cut(s) 147
EcoICRI GAGCTC 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 22
EcoT14I CCWWGG 2 cut(s) 111, 330
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 2 cut(s) 111, 330
FaeI CATG 3 cut(s) 115, 334, 389
FaiI YATR 6 cut(s) 113, 141, 204, 332, 351, 387
FatI CATG 3 cut(s) 111, 330, 385
Fnu4HI GCNGC 1 cut(s) 135
FokI GGATG 1 cut(s) 137
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 1 cut(s) 135
FspBI CTAG 1 cut(s) 158
GluI GCNGC 1 cut(s) 135
GsaI CCCAGC 1 cut(s) 9
HaeIII GGCC 3 cut(s) 174, 241, 329
HapII CCGG 1 cut(s) 148
Hin1II CATG 3 cut(s) 115, 334, 389
HpaII CCGG 1 cut(s) 148
Hpy188I TCNGA 3 cut(s) 182, 397, 414
Hpy188III TCNNGA 2 cut(s) 74, 122
HpyAV CCTTC 4 cut(s) 9, 71, 252, 265
HpyCH4III ACNGT 2 cut(s) 71, 368
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 1 cut(s) 266
Hsp92II CATG 3 cut(s) 115, 334, 389
Kzo9I GATC 2 cut(s) 50, 251
LmnI GCTCC 1 cut(s) 334
LpnPI CCDG 6 cut(s) 9, 36, 107, 161, 199, 303
Lsp1109I GCAGC 1 cut(s) 146
MabI ACCWGGT 1 cut(s) 22
MaeI CTAG 1 cut(s) 158
MalI GATC 2 cut(s) 52, 253
MboI GATC 2 cut(s) 50, 251
MboII GAAGA 4 cut(s) 57, 60, 250, 335
MflI RGATCY 1 cut(s) 251
MhlI GDGCHC 2 cut(s) 339, 349
MluCI AATT 3 cut(s) 230, 307, 353
MmeI TCCRAC 1 cut(s) 392
MnlI CCTC 4 cut(s) 48, 231, 307, 319
MspI CCGG 1 cut(s) 148
MspR9I CCNGG 3 cut(s) 24, 148, 149
MvaI CCWGG 1 cut(s) 24
MvnI CGCG 1 cut(s) 341
MwoI GCNNNNNNNGC 1 cut(s) 143
NciI CCSGG 2 cut(s) 148, 149
NcoI CCATGG 2 cut(s) 111, 330
NdeII GATC 2 cut(s) 50, 251
NlaIII CATG 3 cut(s) 115, 334, 389
NlaIV GGNNCC 1 cut(s) 253
PceI AGGCCT 1 cut(s) 241
PkrI GCNGC 1 cut(s) 136
Psp124BI GAGCTC 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 22
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 1 cut(s) 22
PspN4I GGNNCC 1 cut(s) 253
PspPI GGNCC 1 cut(s) 414
PstNI CAGNNNCTG 1 cut(s) 186
PsuI RGATCY 1 cut(s) 251
SacI GAGCTC 1 cut(s) 339
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 2 cut(s) 50, 251
Sau96I GGNCC 1 cut(s) 414
ScrFI CCNGG 3 cut(s) 24, 148, 149
SduI GDGCHC 2 cut(s) 339, 349
SetI ASST 3 cut(s) 25, 139, 339
SexAI ACCWGGT 1 cut(s) 22
SinI GGWCC 1 cut(s) 414
SmaI CCCGGG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 190
SmoI CTYRAG 1 cut(s) 190
Sse9I AATT 3 cut(s) 230, 307, 353
SseBI AGGCCT 1 cut(s) 241
SspMI CTAG 1 cut(s) 158
SstI GAGCTC 1 cut(s) 339
StuI AGGCCT 1 cut(s) 241
StyD4I CCNGG 3 cut(s) 22, 146, 147
StyI CCWWGG 2 cut(s) 111, 330
TaaI ACNGT 2 cut(s) 71, 368
TasI AATT 3 cut(s) 230, 307, 353
TscAI CASTG 1 cut(s) 371
TseI GCWGC 1 cut(s) 134
TspDTI ATGAA 4 cut(s) 144, 291, 347, 374
TspMI CCCGGG 1 cut(s) 147
TspRI CASTG 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 414
XapI RAATTY 2 cut(s) 307, 353
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
XmaI CCCGGG 1 cut(s) 147
XspI CTAG 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.