Rh4BG011600

RING-like zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0019308
Physical Location & Seq
Forward (+)
1635130 .. 1635854
725 bp
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UTR
Exon/CDS
Intron
Rh4BG011600.1

Sequence Viewer

Length: 450 bp
ATGGCTGGGTTTGGAAGGCTACCTGGTGTTGGTCTGCCTGCAAGAAGAAGATCAAACCACACCCACCACCGTCAAGAAGGAAATCAATGGTTCAAGCCATCAGTGACTTCCATGGATATTCTGGATGATGAAGCAGCTCATAAAGCCCGGGAAAGACTAGAGAAAAGGCTTGGCCGTTTCAGACCCTGCTCAAGGAGTAGAAGCAGCAACCATTATGCCAAAATGGGTTCAAGTTCAAAGCAATTAGGGAGGCCTTCACGGGGATCCTTCTTCTCTCTCAGCAGAAATGAAATAGATGTTTGTGCTATTTGCTTGGAGAATTTTGAGGCAGGAGAAGAGGCCATGGAGCTCGCGTGTGCCCACAAATTTCATTCCCATTGTCTTTCGCCTTGGCTTCATGCCCATTCGGATTGCCCCTATTGTCGGACTAATGTTTCTGTTTGTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

149

Amino Acids

16.81

Weight (kDa)

9.2

Isoelectric Point (pI)

68.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 100 - 142 2.5e-14 Ring finger domain
zf-RING_11 PF17123 101 - 128 6.6e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 101 - 141 8.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 101 - 141 3.9e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019308)

Species Orthologous Gene IDs
prunus_persica Prupe.1G020100_v2.0.a1 Prupe.1G020100_v2.0.a1
pyrus_communis pycom13g17220
rosa_roxburghii Rroxscaffold_5G00334240
rosa_rugosa Rorug03G0310700
rosa_samantha Rh4AG016800 Rh4BG011600 Rh4DG012500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 353
AclWI GGATC 2 cut(s) 258, 271
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 2 cut(s) 319, 365
AfiI CCNNNNNNNGG 4 cut(s) 29, 192, 260, 423
AgsI TTSAA 3 cut(s) 94, 231, 237
AjnI CCWGG 1 cut(s) 22
AluBI AGCT 2 cut(s) 137, 349
AluI AGCT 2 cut(s) 137, 349
Alw21I GWGCWC 1 cut(s) 351
AlwI GGATC 2 cut(s) 258, 271
AlwNI CAGNNNCTG 1 cut(s) 186
Ama87I CYCGRG 1 cut(s) 147
AoxI GGCC 3 cut(s) 172, 251, 339
ApeKI GCWGC 2 cut(s) 134, 204
ApoI RAATTY 2 cut(s) 319, 365
AsuC2I CCSGG 2 cut(s) 148, 149
AvaI CYCGRG 1 cut(s) 147
BaeGI GKGCMC 1 cut(s) 361
BamHI GGATCC 1 cut(s) 263
BanII GRGCYC 1 cut(s) 351
Bbv12I GWGCWC 1 cut(s) 351
BbvI GCAGC 2 cut(s) 146, 216
BccI CCATC 1 cut(s) 106
BceAI ACGGC 1 cut(s) 159
BciT130I CCWGG 1 cut(s) 24
BcnI CCSGG 2 cut(s) 148, 149
BfaI CTAG 1 cut(s) 158
BisI GCNGC 2 cut(s) 135, 205
BlsI GCNGC 2 cut(s) 136, 206
Bme1390I CCNGG 3 cut(s) 24, 148, 149
BmeT110I CYCGRG 1 cut(s) 147
BmiI GGNNCC 1 cut(s) 265
BmrFI CCNGG 3 cut(s) 24, 148, 149
BpuEI CTTGAG 1 cut(s) 175
BpuMI CCSGG 2 cut(s) 148, 149
BsaJI CCNNGG 4 cut(s) 111, 147, 342, 389
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bsc4I CCNNNNNNNGG 4 cut(s) 29, 192, 260, 423
BseBI CCWGG 1 cut(s) 24
BseDI CCNNGG 4 cut(s) 111, 147, 342, 389
BseGI GGATG 1 cut(s) 130
BseLI CCNNNNNNNGG 4 cut(s) 29, 192, 260, 423
BseMII CTCAG 1 cut(s) 292
BseSI GKGCMC 1 cut(s) 361
BseXI GCAGC 2 cut(s) 146, 216
BseYI CCCAGC 1 cut(s) 5
Bsh1236I CGCG 1 cut(s) 353
BshFI GGCC 3 cut(s) 174, 253, 341
BsiHKAI GWGCWC 1 cut(s) 351
BsiHKCI CYCGRG 1 cut(s) 147
BsiSI CCGG 1 cut(s) 148
BslI CCNNNNNNNGG 4 cut(s) 29, 192, 260, 423
BsnI GGCC 3 cut(s) 174, 253, 341
BsoBI CYCGRG 1 cut(s) 147
Bsp1286I GDGCHC 2 cut(s) 351, 361
Bsp143I GATC 2 cut(s) 50, 263
Bsp19I CCATGG 2 cut(s) 111, 342
BspANI GGCC 3 cut(s) 174, 253, 341
BspCNI CTCAG 1 cut(s) 291
BspFNI CGCG 1 cut(s) 353
BspLI GGNNCC 1 cut(s) 265
BspPI GGATC 2 cut(s) 258, 271
BssECI CCNNGG 4 cut(s) 111, 147, 342, 389
BssMI GATC 2 cut(s) 50, 263
BssT1I CCWWGG 3 cut(s) 111, 342, 389
Bst2UI CCWGG 1 cut(s) 24
Bst4CI ACNGT 1 cut(s) 71
Bst6I CTCTTC 1 cut(s) 330
BstC8I GCNNGC 2 cut(s) 39, 351
BstDEI CTNAG 1 cut(s) 278
BstDSI CCRYGG 2 cut(s) 111, 342
BstENI CCTNNNNNAGG 1 cut(s) 190
BstF5I GGATG 1 cut(s) 130
BstFNI CGCG 1 cut(s) 353
BstKTI GATC 2 cut(s) 53, 266
BstMBI GATC 2 cut(s) 50, 263
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstNI CCWGG 1 cut(s) 24
BstSCI CCNGG 3 cut(s) 22, 146, 147
BstSLI GKGCMC 1 cut(s) 361
BstUI CGCG 1 cut(s) 353
BstV1I GCAGC 2 cut(s) 146, 216
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
BsuRI GGCC 3 cut(s) 174, 253, 341
BtgI CCRYGG 2 cut(s) 111, 342
BtsCI GGATG 1 cut(s) 130
BtsIMutI CAGTG 1 cut(s) 108
Cac8I GCNNGC 2 cut(s) 39, 351
CaiI CAGNNNCTG 1 cut(s) 186
Cfr9I CCCGGG 1 cut(s) 147
CsiI ACCWGGT 1 cut(s) 22
CviAII CATG 3 cut(s) 112, 343, 398
DdeI CTNAG 1 cut(s) 278
DpnI GATC 2 cut(s) 52, 265
DpnII GATC 2 cut(s) 50, 263
EaeI YGGCCR 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Ecl136II GAGCTC 1 cut(s) 349
Eco130I CCWWGG 3 cut(s) 111, 342, 389
Eco147I AGGCCT 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 351
Eco53kI GAGCTC 1 cut(s) 349
Eco88I CYCGRG 1 cut(s) 147
EcoICRI GAGCTC 1 cut(s) 349
EcoNI CCTNNNNNAGG 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 22
EcoT14I CCWWGG 3 cut(s) 111, 342, 389
EcoT38I GRGCYC 1 cut(s) 351
ErhI CCWWGG 3 cut(s) 111, 342, 389
FaeI CATG 3 cut(s) 115, 346, 401
FaiI YATR 5 cut(s) 113, 141, 216, 344, 399
FatI CATG 3 cut(s) 111, 342, 397
Fnu4HI GCNGC 2 cut(s) 135, 205
FokI GGATG 1 cut(s) 137
FriOI GRGCYC 1 cut(s) 351
Fsp4HI GCNGC 2 cut(s) 135, 205
FspBI CTAG 1 cut(s) 158
GluI GCNGC 2 cut(s) 135, 205
GsaI CCCAGC 1 cut(s) 9
HaeIII GGCC 3 cut(s) 174, 253, 341
HapII CCGG 1 cut(s) 148
Hin1II CATG 3 cut(s) 115, 346, 401
HpaII CCGG 1 cut(s) 148
Hpy188I TCNGA 3 cut(s) 182, 409, 426
Hpy188III TCNNGA 2 cut(s) 74, 122
HpyAV CCTTC 4 cut(s) 9, 71, 264, 277
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4V TGCA 1 cut(s) 41
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 1 cut(s) 278
Hsp92II CATG 3 cut(s) 115, 346, 401
Kzo9I GATC 2 cut(s) 50, 263
LmnI GCTCC 1 cut(s) 346
LpnPI CCDG 7 cut(s) 9, 36, 51, 107, 161, 199, 315
Lsp1109I GCAGC 2 cut(s) 146, 216
MabI ACCWGGT 1 cut(s) 22
MaeI CTAG 1 cut(s) 158
MaeIII GTNAC 1 cut(s) 103
MalI GATC 2 cut(s) 52, 265
MboI GATC 2 cut(s) 50, 263
MboII GAAGA 4 cut(s) 57, 60, 262, 347
MflI RGATCY 1 cut(s) 263
MhlI GDGCHC 2 cut(s) 351, 361
MluCI AATT 3 cut(s) 242, 319, 365
MmeI TCCRAC 1 cut(s) 404
MnlI CCTC 3 cut(s) 243, 319, 331
MspI CCGG 1 cut(s) 148
MspR9I CCNGG 3 cut(s) 24, 148, 149
MvaI CCWGG 1 cut(s) 24
MvnI CGCG 1 cut(s) 353
MwoI GCNNNNNNNGC 1 cut(s) 143
NciI CCSGG 2 cut(s) 148, 149
NcoI CCATGG 2 cut(s) 111, 342
NdeII GATC 2 cut(s) 50, 263
NlaIII CATG 3 cut(s) 115, 346, 401
NlaIV GGNNCC 1 cut(s) 265
NmuCI GTSAC 1 cut(s) 103
PceI AGGCCT 1 cut(s) 253
PkrI GCNGC 2 cut(s) 136, 206
Psp124BI GAGCTC 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 22
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 1 cut(s) 22
PspN4I GGNNCC 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 186
PsuI RGATCY 1 cut(s) 263
SacI GAGCTC 1 cut(s) 351
SatI GCNGC 2 cut(s) 135, 205
Sau3AI GATC 2 cut(s) 50, 263
ScrFI CCNGG 3 cut(s) 24, 148, 149
SduI GDGCHC 2 cut(s) 351, 361
SetI ASST 3 cut(s) 25, 139, 351
SexAI ACCWGGT 1 cut(s) 22
SmaI CCCGGG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 190
SmoI CTYRAG 1 cut(s) 190
Sse9I AATT 3 cut(s) 242, 319, 365
SseBI AGGCCT 1 cut(s) 253
SspMI CTAG 1 cut(s) 158
SstI GAGCTC 1 cut(s) 351
StuI AGGCCT 1 cut(s) 253
StyD4I CCNGG 3 cut(s) 22, 146, 147
StyI CCWWGG 3 cut(s) 111, 342, 389
TaaI ACNGT 1 cut(s) 71
TasI AATT 3 cut(s) 242, 319, 365
TscAI CASTG 1 cut(s) 108
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 2 cut(s) 134, 204
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 4 cut(s) 144, 303, 359, 386
TspMI CCCGGG 1 cut(s) 147
TspRI CASTG 1 cut(s) 108
XagI CCTNNNNNAGG 1 cut(s) 190
XapI RAATTY 2 cut(s) 319, 365
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
XmaI CCCGGG 1 cut(s) 147
XspI CTAG 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.