Rroxscaffold_5G00363580

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011638
Physical Location & Seq
Reverse (-)
44854557 .. 44860848
6292 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00363580.1

Sequence Viewer

Length: 1557 bp
ATGGGTGGGTGCTTTCCTTGCTTTGGATCATCCAACAAGGAGGGTAGTGGGGGTGGTGGTGCTGGTGTGAAGGAAGTTAGCAAGAAGGAATCAGTTAAAGAAGGCTCAGCTACTCAGTCTCACCATGTTACCAGAGTTAGCTCAGATAAATCAAAATCTCGGAATGGTTCTGATCCTAAGAAGGAACCACCTATTCCAAAAGATGTACCTACAGCACACATTGCTGCACAAACATTTACTTTCCGGGAGCTTGCAGCTGCAACAAAGAATTTTAGGCCCGAGTGTTTATTGGGTGAAGGTGGTTTTGGACGTGTCTACAAAGGTCGCTTGGAGAGCACAGGGCAGGTAGTTGCAGTGAAACAACTTGACAGGAATGGACTGCAAGGAAATCGAGAATTTTTGGTGGAGGTTCTTATGCTTAGCCTTCTGCACCATCCAAACCTTGTCAATTTGATTGGCTACTGTGCTGATGGGGATCAACGCCTTCTTGTTTATGAGTTTATGCCTTTGGGATCCTTGGAGGATCATTTACACGATATTCCAACAGAAAAGGAACCCCTCGACTGGAATACAAGAATGAAGATTGCAGCTGGTGCAGCTAAGGGGTTAGAATATTTGCATGATAAAGCAAACCCGCCCGTTATATACAGAGACTTGAAATCCTCCAACATCCTTCTTGATGAAGACTTTCACCCAAAATTGTCTGATTTTGGGCTTGCAAAGCTAGGTCCTGTTGGTGACAAAACACATGTCTCCACGCGTGTGATGGGGACATATGGCTACTGTGCTCCAGAATATGCTATGACAGGCCAGCTCACTCTGAAATCCGATGTGTACAGTTTTGGAGTTGTCTTTCTCGAACTTATAACAGGACGAAAAGCTATTGATAACACCCGTGGTCCTGGAGAGCATAATCTTGTTGCATGGGCACGACCACTTTTTAAAGATCGTAGGAAGTTCCCAAAAATGGCTGATCCACTACTACAGGGCCGTTATCCAATGCGGGGGCTTTACCAAGCTCTTGCTGTTGCAGCCATGTGCTTGCAGGAGCAAGCAGCTACAAGGCCTTTAATAGGAGATGTTGTGACTGCTCTTACATACTTGGCTTCTCAAACCTATGATCCCAATGCAGCTAGTGGTCACAGTAACAGAGTTGGTCCATCTACTCCTAGGCACAAGGACGATCGAAGGAACATGGGAGATGGGCTGGATAGCCCAGATGAGCCTGGACGAGGTGGACGGCATGGTAGCCATTCATACAGAAACTCACCTGATTTCAGAAGGAAAGATCCCAATAGGGACTTGGGTGCTGGTGTAGAATTAGGGAGGATTGAAACCGGTAGCGGCTCTGGGAGGAAATGGGGCCTAGATGGGTTAGAGCAACAAGATTCTCAGAGGGACAGCCCTGTAAGTGCTGGGAGAGCAAGGGAAACTCCGCGAAATCGTGATCTAGATAGAGAACGTGCAGTGGCTGAGGCGAAGGTTTGGGGCGAAAACTGGAGAGAAAAGAAAAGAGCAAATGTGATGGGTGGTAGCTTTGATGGTACAAATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000187 GO:0001932 GO:0001934 GO:0002218 GO:0002221 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002757 GO:0002758 GO:0002764 GO:0002831 GO:0002833 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009814 GO:0009816 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0023052 GO:0023056 GO:0031224 GO:0031225 GO:0031226 GO:0031323 GO:0031325 GO:0031347 GO:0031349 GO:0031399 GO:0031401 GO:0032101 GO:0032103 GO:0032147 GO:0032268 GO:0032270 GO:0033674 GO:0035690 GO:0036211 GO:0038023 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0043902 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0045859 GO:0045860 GO:0045937 GO:0046658 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0098542 GO:0140096 GO:1900150 GO:1900424 GO:1900426 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

518

Amino Acids

56.76

Weight (kDa)

8.9

Isoelectric Point (pI)

33.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 96 - 303 1.2e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 96 - 296 5.5e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011638)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18610 AT5G18610 AT5G18610
fragaria_vesca FvH4_4g17410 FvH4_4g17410
malus_domestica MD13G1172400.v1.1 MD16G1173800.v1.1
prunus_persica Prupe.1G140600_v2.0.a1
pyrus_communis pycom13g14790 pycom16g14560
rosa_chinensis RchiOBHm_Chr4g0421331
rosa_laevigata RLG00000007696
rosa_multiflora Rmu_sc0015713.1_g000007
rosa_roxburghii Rroxscaffold_5G00363580
rosa_rugosa Rorug04G0168900 Rorug04G0168900
rosa_samantha Rh4BG234000 Rh4DG229700
rosa_wichuraiana Rw4G020290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 866
Acc36I ACCTGC 1 cut(s) 334
AccI GTMKAC 1 cut(s) 315
AccII CGCG 2 cut(s) 760, 1438
AciI CCGC 4 cut(s) 635, 1003, 1344, 1436
AclWI GGATC 9 cut(s) 34, 167, 483, 507, 520, 531, 968, 1115, 1283
AcsI RAATTY 2 cut(s) 268, 395
AfaI GTAC 3 cut(s) 207, 836, 1546
AfiI CCNNNNNNNGG 6 cut(s) 23, 564, 967, 1004, 1073, 1232
AflIII ACRYGT 3 cut(s) 310, 748, 758
AgeI ACCGGT 1 cut(s) 1337
AgsI TTSAA 2 cut(s) 658, 1334
AjiI CACGTC 1 cut(s) 311
AjnI CCWGG 2 cut(s) 901, 1225
AjuI GAANNNNNNNTTGG 2 cut(s) 288, 320
AleI CACNNNNGTG 1 cut(s) 761
Alw21I GWGCWC 2 cut(s) 338, 790
Alw26I GTCTC 3 cut(s) 123, 645, 757
AlwI GGATC 9 cut(s) 34, 167, 483, 507, 520, 531, 968, 1115, 1283
AlwNI CAGNNNCTG 1 cut(s) 1472
Ama87I CYCGRG 1 cut(s) 278
AoxI GGCC 5 cut(s) 275, 808, 988, 1064, 1363
ApeKI GCWGC 8 cut(s) 224, 254, 257, 587, 596, 1031, 1055, 1130
ApoI RAATTY 2 cut(s) 268, 395
ArsI GACNNNNNNTTYG 2 cut(s) 735, 767
AsiGI ACCGGT 1 cut(s) 1337
Asp700I GAANNNNTTC 1 cut(s) 687
AspA2I CCTAGG 1 cut(s) 1169
AspS9I GGNCC 6 cut(s) 276, 728, 899, 988, 1157, 1363
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 5 cut(s) 113, 305, 683, 749, 1260
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 3 cut(s) 728, 899, 1157
AvrII CCTAGG 1 cut(s) 1169
BaeGI GKGCMC 1 cut(s) 931
BamHI GGATCC 1 cut(s) 512
BbsI GAAGAC 1 cut(s) 690
Bbv12I GWGCWC 2 cut(s) 338, 790
BbvCI CCTCAGC 1 cut(s) 1473
BbvI GCAGC 8 cut(s) 211, 244, 266, 599, 608, 1043, 1067, 1142
BccI CCATC 8 cut(s) 441, 464, 760, 1168, 1196, 1364, 1519, 1535
BceAI ACGGC 2 cut(s) 975, 1256
BcgI CGANNNNNNTGC 2 cut(s) 371, 405
BciT130I CCWGG 2 cut(s) 903, 1227
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 3 cut(s) 123, 645, 757
BfaI CTAG 5 cut(s) 725, 1134, 1170, 1367, 1451
BfmI CTRYAG 2 cut(s) 210, 983
BfuAI ACCTGC 1 cut(s) 334
BisI GCNGC 9 cut(s) 225, 255, 258, 588, 597, 1032, 1056, 1131, 1345
BlnI CCTAGG 1 cut(s) 1169
BlpI GCTNAGC 2 cut(s) 106, 419
BlsI GCNGC 9 cut(s) 226, 256, 259, 589, 598, 1033, 1057, 1132, 1346
Bme1390I CCNGG 3 cut(s) 245, 903, 1227
Bme18I GGWCC 3 cut(s) 728, 899, 1157
BmeT110I CYCGRG 1 cut(s) 278
BmgBI CACGTC 1 cut(s) 311
BmgT120I GGNCC 6 cut(s) 276, 728, 899, 988, 1157, 1363
BmiI GGNNCC 4 cut(s) 186, 514, 555, 1364
BmrFI CCNGG 3 cut(s) 245, 903, 1227
BpiI GAAGAC 1 cut(s) 690
BpmI CTGGAG 3 cut(s) 774, 924, 1519
Bpu10I CCTNAGC 2 cut(s) 600, 1473
Bpu1102I GCTNAGC 2 cut(s) 106, 419
BpuMI CCSGG 1 cut(s) 245
BsaBI GATNNNNATC 1 cut(s) 474
BsaJI CCNNGG 3 cut(s) 516, 895, 1169
BsaWI WCCGGW 1 cut(s) 1337
BsaXI ACNNNNNCTCC 2 cut(s) 1068, 1098
Bsc4I CCNNNNNNNGG 6 cut(s) 23, 564, 967, 1004, 1073, 1232
Bse118I RCCGGY 1 cut(s) 1337
Bse1I ACTGG 2 cut(s) 569, 1502
Bse3DI GCAATG 1 cut(s) 219
Bse8I GATNNNNATC 1 cut(s) 474
BseBI CCWGG 2 cut(s) 903, 1227
BseDI CCNNGG 3 cut(s) 516, 895, 1169
BseGI GGATG 3 cut(s) 29, 433, 669
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 6 cut(s) 23, 564, 967, 1004, 1073, 1232
BseMI GCAATG 1 cut(s) 219
BseMII CTCAG 5 cut(s) 120, 128, 156, 1406, 1464
BseNI ACTGG 2 cut(s) 569, 1502
BseSI GKGCMC 1 cut(s) 931
BseXI GCAGC 8 cut(s) 211, 244, 266, 599, 608, 1043, 1067, 1142
BseYI CCCAGC 1 cut(s) 1415
BsgI GTGCAG 4 cut(s) 210, 413, 615, 1485
Bsh1236I CGCG 2 cut(s) 760, 1438
Bsh1285I CGRYCG 1 cut(s) 1186
BshFI GGCC 5 cut(s) 277, 810, 990, 1066, 1365
BshTI ACCGGT 1 cut(s) 1337
BsiEI CGRYCG 1 cut(s) 1186
BsiHKAI GWGCWC 2 cut(s) 338, 790
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 2 cut(s) 244, 1338
BslFI GGGAC 3 cut(s) 784, 1313, 1412
BslI CCNNNNNNNGG 6 cut(s) 23, 564, 967, 1004, 1073, 1232
BsmAI GTCTC 3 cut(s) 123, 645, 757
BsmFI GGGAC 3 cut(s) 784, 1313, 1412
BsnI GGCC 5 cut(s) 277, 810, 990, 1066, 1365
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 3 cut(s) 338, 790, 931
Bsp1407I TGTACA 1 cut(s) 834
Bsp1720I GCTNAGC 2 cut(s) 106, 419
BspACI CCGC 4 cut(s) 635, 1003, 1344, 1436
BspANI GGCC 5 cut(s) 277, 810, 990, 1066, 1365
BspCNI CTCAG 5 cut(s) 119, 127, 155, 1405, 1465
BspFNI CGCG 2 cut(s) 760, 1438
BspLI GGNNCC 4 cut(s) 186, 514, 555, 1364
BspMI ACCTGC 1 cut(s) 334
BspPI GGATC 9 cut(s) 34, 167, 483, 507, 520, 531, 968, 1115, 1283
BsrDI GCAATG 1 cut(s) 219
BsrFI RCCGGY 1 cut(s) 1337
BsrGI TGTACA 1 cut(s) 834
BsrI ACTGG 2 cut(s) 569, 1502
BssAI RCCGGY 1 cut(s) 1337
BssECI CCNNGG 3 cut(s) 516, 895, 1169
BssT1I CCWWGG 2 cut(s) 516, 1169
Bst2UI CCWGG 2 cut(s) 903, 1227
Bst4CI ACNGT 4 cut(s) 464, 785, 839, 1145
BstAPI GCANNNNNTGC 2 cut(s) 221, 593
BstAUI TGTACA 1 cut(s) 834
BstC8I GCNNGC 5 cut(s) 252, 717, 812, 1043, 1053
BstDEI CTNAG 8 cut(s) 106, 114, 142, 177, 419, 600, 1392, 1473
BstDSI CCRYGG 1 cut(s) 895
BstENI CCTNNNNNAGG 2 cut(s) 1071, 1230
BstF5I GGATG 3 cut(s) 29, 433, 669
BstFNI CGCG 2 cut(s) 760, 1438
BstMAI GTCTC 3 cut(s) 123, 645, 757
BstMCI CGRYCG 1 cut(s) 1186
BstMWI GCNNNNNNNGC 8 cut(s) 18, 221, 333, 593, 596, 721, 1031, 1421
BstNI CCWGG 2 cut(s) 903, 1227
BstNSI RCATGY 1 cut(s) 752
BstSCI CCNGG 3 cut(s) 243, 901, 1225
BstSFI CTRYAG 2 cut(s) 210, 983
BstSLI GKGCMC 1 cut(s) 931
BstUI CGCG 2 cut(s) 760, 1438
BstV1I GCAGC 8 cut(s) 211, 244, 266, 599, 608, 1043, 1067, 1142
BstV2I GAAGAC 1 cut(s) 690
BstX2I RGATCY 2 cut(s) 512, 1288
BstYI RGATCY 2 cut(s) 512, 1288
BsuRI GGCC 5 cut(s) 277, 810, 990, 1066, 1365
BtgI CCRYGG 1 cut(s) 895
BtrI CACGTC 1 cut(s) 311
BtsCI GGATG 3 cut(s) 29, 433, 669
BtsI GCAGTG 2 cut(s) 360, 1473
BtsIMutI CAGTG 2 cut(s) 360, 1473
BveI ACCTGC 1 cut(s) 334
Cac8I GCNNGC 5 cut(s) 252, 717, 812, 1043, 1053
CaiI CAGNNNCTG 1 cut(s) 1472
Cfr10I RCCGGY 1 cut(s) 1337
Cfr13I GGNCC 6 cut(s) 276, 728, 899, 988, 1157, 1363
Csp6I GTAC 3 cut(s) 206, 835, 1545
CspAI ACCGGT 1 cut(s) 1337
CviAII CATG 7 cut(s) 125, 620, 749, 924, 1036, 1195, 1244
CviQI GTAC 3 cut(s) 206, 835, 1545
DdeI CTNAG 8 cut(s) 106, 114, 142, 177, 419, 600, 1392, 1473
DraI TTTAAA 1 cut(s) 943
Eco130I CCWWGG 2 cut(s) 516, 1169
Eco147I AGGCCT 1 cut(s) 1066
Eco47I GGWCC 3 cut(s) 728, 899, 1157
Eco88I CYCGRG 1 cut(s) 278
EcoNI CCTNNNNNAGG 2 cut(s) 1071, 1230
EcoO109I RGGNCCY 2 cut(s) 728, 1363
EcoRII CCWGG 2 cut(s) 901, 1225
EcoT14I CCWWGG 2 cut(s) 516, 1169
ErhI CCWWGG 2 cut(s) 516, 1169
FaeI CATG 7 cut(s) 128, 623, 752, 927, 1039, 1198, 1247
FaqI GGGAC 3 cut(s) 784, 1313, 1412
FatI CATG 7 cut(s) 124, 619, 748, 923, 1035, 1194, 1243
FauI CCCGC 2 cut(s) 642, 996
FauNDI CATATG 1 cut(s) 775
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 9 cut(s) 225, 255, 258, 588, 597, 1032, 1056, 1131, 1345
FokI GGATG 3 cut(s) 16, 420, 656
Fsp4HI GCNGC 9 cut(s) 225, 255, 258, 588, 597, 1032, 1056, 1131, 1345
FspBI CTAG 5 cut(s) 725, 1134, 1170, 1367, 1451
GluI GCNGC 9 cut(s) 225, 255, 258, 588, 597, 1032, 1056, 1131, 1345
GsaI CCCAGC 1 cut(s) 1419
GsuI CTGGAG 3 cut(s) 774, 924, 1519
HaeIII GGCC 5 cut(s) 277, 810, 990, 1066, 1365
HapII CCGG 2 cut(s) 244, 1338
Hin1II CATG 7 cut(s) 128, 623, 752, 927, 1039, 1198, 1247
HinfI GANTC 2 cut(s) 89, 1388
HpaII CCGG 2 cut(s) 244, 1338
HphI GGTGA 5 cut(s) 113, 305, 683, 749, 1260
Hpy166II GTNNAC 3 cut(s) 316, 835, 1238
Hpy188I TCNGA 8 cut(s) 145, 162, 172, 706, 822, 829, 1280, 1395
Hpy188III TCNNGA 6 cut(s) 392, 677, 791, 857, 1445, 1451
Hpy8I GTNNAC 3 cut(s) 316, 835, 1238
HpyCH4III ACNGT 4 cut(s) 464, 785, 839, 1145
HpyCH4IV ACGT 2 cut(s) 310, 1462
HpyF10VI GCNNNNNNNGC 8 cut(s) 18, 221, 333, 593, 596, 721, 1031, 1421
HpyF3I CTNAG 8 cut(s) 106, 114, 142, 177, 419, 600, 1392, 1473
HpySE526I ACGT 2 cut(s) 310, 1462
Hsp92II CATG 7 cut(s) 128, 623, 752, 927, 1039, 1198, 1247
LmnI GCTCC 3 cut(s) 247, 793, 1048
Lsp1109I GCAGC 8 cut(s) 211, 244, 266, 599, 608, 1043, 1067, 1142
MaeI CTAG 5 cut(s) 725, 1134, 1170, 1367, 1451
MaeII ACGT 2 cut(s) 310, 1462
MaeIII GTNAC 5 cut(s) 127, 737, 1084, 1139, 1145
MboII GAAGA 2 cut(s) 592, 695
MflI RGATCY 2 cut(s) 512, 1288
MhlI GDGCHC 3 cut(s) 338, 790, 931
MluCI AATT 5 cut(s) 268, 395, 448, 698, 1319
MluI ACGCGT 1 cut(s) 758
MmeI TCCRAC 3 cut(s) 57, 566, 690
MroXI GAANNNNTTC 1 cut(s) 687
MseI TTAA 3 cut(s) 96, 942, 1070
MslI CAYNNNNRTG 1 cut(s) 761
MspA1I CMGCKG 2 cut(s) 257, 590
MspI CCGG 2 cut(s) 244, 1338
MspR9I CCNGG 3 cut(s) 245, 903, 1227
MvaI CCWGG 2 cut(s) 903, 1227
MvnI CGCG 2 cut(s) 760, 1438
MwoI GCNNNNNNNGC 8 cut(s) 18, 221, 333, 593, 596, 721, 1031, 1421
NciI CCSGG 1 cut(s) 245
NdeI CATATG 1 cut(s) 775
NlaIII CATG 7 cut(s) 128, 623, 752, 927, 1039, 1198, 1247
NlaIV GGNNCC 4 cut(s) 186, 514, 555, 1364
NmuCI GTSAC 3 cut(s) 737, 1084, 1139
NspI RCATGY 1 cut(s) 752
OliI CACNNNNGTG 1 cut(s) 761
PceI AGGCCT 1 cut(s) 1066
PciI ACATGT 1 cut(s) 748
PdmI GAANNNNTTC 1 cut(s) 687
PfeI GAWTC 2 cut(s) 89, 1388
PfoI TCCNGGA 2 cut(s) 243, 901
PinAI ACCGGT 1 cut(s) 1337
PkrI GCNGC 9 cut(s) 226, 256, 259, 589, 598, 1033, 1057, 1132, 1346
Ple19I CGATCG 1 cut(s) 1186
PpuMI RGGWCCY 1 cut(s) 728
PscI ACATGT 1 cut(s) 748
PsiI TTATAA 1 cut(s) 866
Psp5II RGGWCCY 1 cut(s) 728
Psp6I CCWGG 2 cut(s) 901, 1225
PspFI CCCAGC 1 cut(s) 1415
PspGI CCWGG 2 cut(s) 901, 1225
PspN4I GGNNCC 4 cut(s) 186, 514, 555, 1364
PspPI GGNCC 6 cut(s) 276, 728, 899, 988, 1157, 1363
PspPPI RGGWCCY 1 cut(s) 728
PstNI CAGNNNCTG 1 cut(s) 1472
PsuI RGATCY 2 cut(s) 512, 1288
PvuI CGATCG 1 cut(s) 1186
PvuII CAGCTG 2 cut(s) 257, 590
RsaI GTAC 3 cut(s) 207, 836, 1546
RsaNI GTAC 3 cut(s) 206, 835, 1545
RseI CAYNNNNRTG 1 cut(s) 761
SaqAI TTAA 3 cut(s) 96, 942, 1070
SatI GCNGC 9 cut(s) 225, 255, 258, 588, 597, 1032, 1056, 1131, 1345
Sau96I GGNCC 6 cut(s) 276, 728, 899, 988, 1157, 1363
ScrFI CCNGG 3 cut(s) 245, 903, 1227
SduI GDGCHC 3 cut(s) 338, 790, 931
SfcI CTRYAG 2 cut(s) 210, 983
SinI GGWCC 3 cut(s) 728, 899, 1157
SmiMI CAYNNNNRTG 1 cut(s) 761
Sse9I AATT 5 cut(s) 268, 395, 448, 698, 1319
SseBI AGGCCT 1 cut(s) 1066
SsiI CCGC 4 cut(s) 635, 1003, 1344, 1436
SspI AATATT 1 cut(s) 614
SspMI CTAG 5 cut(s) 725, 1134, 1170, 1367, 1451
StuI AGGCCT 1 cut(s) 1066
StyD4I CCNGG 3 cut(s) 243, 901, 1225
StyI CCWWGG 2 cut(s) 516, 1169
TaaI ACNGT 4 cut(s) 464, 785, 839, 1145
TaiI ACGT 2 cut(s) 313, 1465
TaqI TCGA 4 cut(s) 391, 561, 858, 1186
TasI AATT 5 cut(s) 268, 395, 448, 698, 1319
TatI WGTACW 1 cut(s) 834
TauI GCSGC 1 cut(s) 1347
TfiI GAWTC 2 cut(s) 89, 1388
Tru1I TTAA 3 cut(s) 96, 942, 1070
Tru9I TTAA 3 cut(s) 96, 942, 1070
TscAI CASTG 2 cut(s) 360, 1473
TseFI GTSAC 3 cut(s) 737, 1084, 1139
TseI GCWGC 8 cut(s) 224, 254, 257, 587, 596, 1031, 1055, 1130
Tsp45I GTSAC 3 cut(s) 737, 1084, 1139
TspDTI ATGAA 3 cut(s) 593, 696, 1245
TspRI CASTG 2 cut(s) 360, 1473
VpaK11BI GGWCC 3 cut(s) 728, 899, 1157
XagI CCTNNNNNAGG 2 cut(s) 1071, 1230
XapI RAATTY 2 cut(s) 268, 395
XbaI TCTAGA 1 cut(s) 1450
XceI RCATGY 1 cut(s) 752
XcmI CCANNNNNNNNNTGG 2 cut(s) 763, 1300
XmaJI CCTAGG 1 cut(s) 1169
XmiI GTMKAC 1 cut(s) 315
XmnI GAANNNNTTC 1 cut(s) 687
XspI CTAG 5 cut(s) 725, 1134, 1170, 1367, 1451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.