Rw4G020290

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011638
Physical Location & Seq
Forward (+)
44113797 .. 44119968
6172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G020290.1

Sequence Viewer

Length: 1551 bp
ATGGGTGGGTGCTTTCCTTGCTTTGGATCATCCAACAAGGAGGGTAGTGGTGGTGCTGGTGTGAAGGAAGTTAGCAAGAAGGAATCAGTTAAAGAAGGCTCAGCTACTCAGTCTCACCATGTTACCAGAGCTGGCTCAGATAAATCAAAATCTCGGAATGGTTCTGATCCTAAAAAGGAACCACCTATTCCAAAAGATGTACCTACAGCACACATTGCTGCACAAACATTTACTTTCCGGGAGCTTGCAGCTGCAACAAAGAATTTTAAGCCCGAGTGTTTATTGGGTGAAGGTGGTTTTGGACGTGTCTACAAAGGTCGCTTGGAGAGCACAGGGCAGGTAGTTGCAGTGAAACAACTTGACAGGAATGGACTGCAAGGAAATCGAGAATTTTTGGTGGAGGTTCTTATGCTTAGCCTTCTGCACCATCCAAACCTTGTCAATTTGATTGGCTACTGTGCTGATGGGGATCAACGCCTTCTTGTTTATGAGTTTATGCCTTTGGGATCCTTGGAGGATCATTTACACGATATTCCAACAGAAAAGGAACCCCTCGACTGGAATACAAGAATGAAGATTGCAGCTGGTGCAGCTAAGGGGTTAGAATATTTGCATGATAAAGCAAACCCGCCCGTTATATACAGAGACTTGAAATCCTCCAACATACTTCTTGATGAAGACTTTCACCCAAAATTGTCTGATTTTGGGCTTGCAAAGCTAGGTCCAGTTGGTGACAAAACACATGTCTCCACGCGTGTGATGGGGACATATGGCTACTGTGCTCCAGAATATGCTATGACAGGCCAGCTCACTCTGAAATCCGATGTGTACAGTTTCGGAGTTGTCTTTCTCGAACTTATAACAGGACGAAAAGCTATTGATAACACCCGTGGTCCTGGAGAGCATAATCTTGTTGCATGGGCACGACCACTTTTTAAAGATCGTAGGAAGTTCCCAAAAATGGCTGATCCACTACTACAGGGCCGTTATCCAATGCGGGGGCTTTACCAAGCTCTTGCTGTTGCAGCCATGTGCTTGCAGGAGCAAGCAGCTACAAGGCCTTTAATAGGAGATGTAGTGACTGCTCTTACATACTTGGCTTCTCAAACCTATGATCCAAATGCAGCTAGTGGTCACAGTAACAGAGTTGGTCCATCTACTCCTAGGCACAAGGATGATCGAAGGAACATGGGAGATGGGCTGGATAGCCCAGATGAGCCTGGAAGAGGTGGACGGCATGGTAGCCATTCATACAGAAACTCACCTGATTTCAGAAGGAAAGATCCCAATAGGGACTTGGGTGCTGGTGTAGAATTAGGAAGGATTGAAACCGGTAACGGCTCTGGAAGGAAATGGGGTCTAGATGGGTTAGAGCAACAAGATTCTCAGAGGGACAGCCCTGTAAGTGCTGGGAGAGCAAGGGAAACTCCGCGAAATCGTGATCTAGATAGAGAACGTGCGGTGGCTGAGGCGAAGGTTTGGGGCGAAAACTGGAGAGAAAAGAAAAGAGCAAATGTGATGGGTGGTAGCTTTGATGGTACAAATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000187 GO:0001932 GO:0001934 GO:0002218 GO:0002221 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002757 GO:0002758 GO:0002764 GO:0002831 GO:0002833 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009719 GO:0009814 GO:0009816 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0023052 GO:0023056 GO:0031224 GO:0031225 GO:0031226 GO:0031323 GO:0031325 GO:0031347 GO:0031349 GO:0031399 GO:0031401 GO:0032101 GO:0032103 GO:0032147 GO:0032268 GO:0032270 GO:0033674 GO:0035690 GO:0036211 GO:0038023 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0042742 GO:0043085 GO:0043170 GO:0043207 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0043902 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0045859 GO:0045860 GO:0045937 GO:0046658 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0098542 GO:0140096 GO:1900150 GO:1900424 GO:1900426 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

516

Amino Acids

56.59

Weight (kDa)

8.89

Isoelectric Point (pI)

31.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 94 - 301 1.1e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 94 - 294 4.6e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011638)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18610 AT5G18610 AT5G18610
fragaria_vesca FvH4_4g17410 FvH4_4g17410
malus_domestica MD13G1172400.v1.1 MD16G1173800.v1.1
prunus_persica Prupe.1G140600_v2.0.a1
pyrus_communis pycom13g14790 pycom16g14560
rosa_chinensis RchiOBHm_Chr4g0421331
rosa_laevigata RLG00000007696
rosa_multiflora Rmu_sc0015713.1_g000007
rosa_roxburghii Rroxscaffold_5G00363580
rosa_rugosa Rorug04G0168900 Rorug04G0168900
rosa_samantha Rh4BG234000 Rh4DG229700
rosa_wichuraiana Rw4G020290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 860
Acc36I ACCTGC 1 cut(s) 328
AccI GTMKAC 1 cut(s) 309
AccII CGCG 2 cut(s) 754, 1432
AciI CCGC 4 cut(s) 629, 997, 1430, 1460
AclWI GGATC 9 cut(s) 34, 161, 477, 501, 514, 525, 962, 1109, 1277
AcsI RAATTY 2 cut(s) 262, 389
AfaI GTAC 3 cut(s) 201, 830, 1540
AfiI CCNNNNNNNGG 6 cut(s) 23, 558, 961, 998, 1067, 1226
AflIII ACRYGT 3 cut(s) 304, 742, 752
AgeI ACCGGT 1 cut(s) 1331
AgsI TTSAA 2 cut(s) 652, 1328
AjiI CACGTC 1 cut(s) 305
AjnI CCWGG 2 cut(s) 895, 1219
AjuI GAANNNNNNNTTGG 2 cut(s) 282, 314
AleI CACNNNNGTG 1 cut(s) 755
Alw21I GWGCWC 2 cut(s) 332, 784
Alw26I GTCTC 3 cut(s) 117, 639, 751
AlwI GGATC 9 cut(s) 34, 161, 477, 501, 514, 525, 962, 1109, 1277
Ama87I CYCGRG 1 cut(s) 272
AoxI GGCC 3 cut(s) 802, 982, 1058
ApeKI GCWGC 8 cut(s) 218, 248, 251, 581, 590, 1025, 1049, 1124
ApoI RAATTY 2 cut(s) 262, 389
ArsI GACNNNNNNTTYG 2 cut(s) 729, 761
AsiGI ACCGGT 1 cut(s) 1331
Asp700I GAANNNNTTC 1 cut(s) 681
AspA2I CCTAGG 1 cut(s) 1163
AspS9I GGNCC 4 cut(s) 722, 893, 982, 1151
AsuC2I CCSGG 1 cut(s) 239
AsuHPI GGTGA 5 cut(s) 107, 299, 677, 743, 1254
AvaI CYCGRG 1 cut(s) 272
AvaII GGWCC 3 cut(s) 722, 893, 1151
AvrII CCTAGG 1 cut(s) 1163
BaeGI GKGCMC 1 cut(s) 925
BamHI GGATCC 1 cut(s) 506
BbsI GAAGAC 1 cut(s) 684
Bbv12I GWGCWC 2 cut(s) 332, 784
BbvCI CCTCAGC 1 cut(s) 1467
BbvI GCAGC 8 cut(s) 205, 238, 260, 593, 602, 1037, 1061, 1136
BccI CCATC 8 cut(s) 435, 458, 754, 1162, 1190, 1358, 1513, 1529
BceAI ACGGC 3 cut(s) 969, 1250, 1354
BcgI CGANNNNNNTGC 2 cut(s) 365, 399
BciT130I CCWGG 2 cut(s) 897, 1221
BcnI CCSGG 1 cut(s) 239
BcoDI GTCTC 3 cut(s) 117, 639, 751
BfaI CTAG 5 cut(s) 719, 1128, 1164, 1361, 1445
BfmI CTRYAG 2 cut(s) 204, 977
BfuAI ACCTGC 1 cut(s) 328
BisI GCNGC 8 cut(s) 219, 249, 252, 582, 591, 1026, 1050, 1125
BlnI CCTAGG 1 cut(s) 1163
BlpI GCTNAGC 2 cut(s) 100, 413
BlsI GCNGC 8 cut(s) 220, 250, 253, 583, 592, 1027, 1051, 1126
Bme1390I CCNGG 3 cut(s) 239, 897, 1221
Bme18I GGWCC 3 cut(s) 722, 893, 1151
BmeT110I CYCGRG 1 cut(s) 272
BmgBI CACGTC 1 cut(s) 305
BmgT120I GGNCC 4 cut(s) 722, 893, 982, 1151
BmiI GGNNCC 3 cut(s) 180, 508, 549
BmrFI CCNGG 3 cut(s) 239, 897, 1221
BpiI GAAGAC 1 cut(s) 684
BpmI CTGGAG 3 cut(s) 768, 918, 1513
Bpu10I CCTNAGC 2 cut(s) 594, 1467
Bpu1102I GCTNAGC 2 cut(s) 100, 413
BpuMI CCSGG 1 cut(s) 239
BsaBI GATNNNNATC 1 cut(s) 468
BsaJI CCNNGG 3 cut(s) 510, 889, 1163
BsaWI WCCGGW 1 cut(s) 1331
BsaXI ACNNNNNCTCC 2 cut(s) 1062, 1092
Bsc4I CCNNNNNNNGG 6 cut(s) 23, 558, 961, 998, 1067, 1226
Bse118I RCCGGY 1 cut(s) 1331
Bse1I ACTGG 3 cut(s) 563, 725, 1496
Bse3DI GCAATG 1 cut(s) 213
Bse8I GATNNNNATC 1 cut(s) 468
BseBI CCWGG 2 cut(s) 897, 1221
BseDI CCNNGG 3 cut(s) 510, 889, 1163
BseGI GGATG 3 cut(s) 29, 427, 1180
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 6 cut(s) 23, 558, 961, 998, 1067, 1226
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 5 cut(s) 114, 122, 150, 1400, 1458
BseNI ACTGG 3 cut(s) 563, 725, 1496
BseSI GKGCMC 1 cut(s) 925
BseXI GCAGC 8 cut(s) 205, 238, 260, 593, 602, 1037, 1061, 1136
BseYI CCCAGC 1 cut(s) 1409
BsgI GTGCAG 3 cut(s) 204, 407, 609
Bsh1236I CGCG 2 cut(s) 754, 1432
BshFI GGCC 3 cut(s) 804, 984, 1060
BshTI ACCGGT 1 cut(s) 1331
BsiHKAI GWGCWC 2 cut(s) 332, 784
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 2 cut(s) 238, 1332
BslFI GGGAC 3 cut(s) 778, 1307, 1406
BslI CCNNNNNNNGG 6 cut(s) 23, 558, 961, 998, 1067, 1226
BsmAI GTCTC 3 cut(s) 117, 639, 751
BsmFI GGGAC 3 cut(s) 778, 1307, 1406
BsnI GGCC 3 cut(s) 804, 984, 1060
BsoBI CYCGRG 1 cut(s) 272
Bsp1286I GDGCHC 3 cut(s) 332, 784, 925
Bsp1407I TGTACA 1 cut(s) 828
Bsp1720I GCTNAGC 2 cut(s) 100, 413
BspACI CCGC 4 cut(s) 629, 997, 1430, 1460
BspANI GGCC 3 cut(s) 804, 984, 1060
BspCNI CTCAG 5 cut(s) 113, 121, 149, 1399, 1459
BspFNI CGCG 2 cut(s) 754, 1432
BspLI GGNNCC 3 cut(s) 180, 508, 549
BspMI ACCTGC 1 cut(s) 328
BspPI GGATC 9 cut(s) 34, 161, 477, 501, 514, 525, 962, 1109, 1277
BsrDI GCAATG 1 cut(s) 213
BsrFI RCCGGY 1 cut(s) 1331
BsrGI TGTACA 1 cut(s) 828
BsrI ACTGG 3 cut(s) 563, 725, 1496
BssAI RCCGGY 1 cut(s) 1331
BssECI CCNNGG 3 cut(s) 510, 889, 1163
BssT1I CCWWGG 2 cut(s) 510, 1163
Bst2UI CCWGG 2 cut(s) 897, 1221
Bst4CI ACNGT 4 cut(s) 458, 779, 833, 1139
Bst6I CTCTTC 1 cut(s) 1219
BstAPI GCANNNNNTGC 2 cut(s) 215, 587
BstAUI TGTACA 1 cut(s) 828
BstC8I GCNNGC 6 cut(s) 133, 246, 711, 806, 1037, 1047
BstDEI CTNAG 7 cut(s) 100, 108, 136, 413, 594, 1386, 1467
BstDSI CCRYGG 1 cut(s) 889
BstENI CCTNNNNNAGG 2 cut(s) 1065, 1224
BstF5I GGATG 3 cut(s) 29, 427, 1180
BstFNI CGCG 2 cut(s) 754, 1432
BstMAI GTCTC 3 cut(s) 117, 639, 751
BstMWI GCNNNNNNNGC 8 cut(s) 18, 215, 327, 587, 590, 715, 1025, 1415
BstNI CCWGG 2 cut(s) 897, 1221
BstNSI RCATGY 1 cut(s) 746
BstSCI CCNGG 3 cut(s) 237, 895, 1219
BstSFI CTRYAG 2 cut(s) 204, 977
BstSLI GKGCMC 1 cut(s) 925
BstUI CGCG 2 cut(s) 754, 1432
BstV1I GCAGC 8 cut(s) 205, 238, 260, 593, 602, 1037, 1061, 1136
BstV2I GAAGAC 1 cut(s) 684
BstX2I RGATCY 2 cut(s) 506, 1282
BstYI RGATCY 2 cut(s) 506, 1282
BsuRI GGCC 3 cut(s) 804, 984, 1060
BtgI CCRYGG 1 cut(s) 889
BtrI CACGTC 1 cut(s) 305
BtsCI GGATG 3 cut(s) 29, 427, 1180
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 1 cut(s) 354
BveI ACCTGC 1 cut(s) 328
Cac8I GCNNGC 6 cut(s) 133, 246, 711, 806, 1037, 1047
Cfr10I RCCGGY 1 cut(s) 1331
Cfr13I GGNCC 4 cut(s) 722, 893, 982, 1151
Csp6I GTAC 3 cut(s) 200, 829, 1539
CspAI ACCGGT 1 cut(s) 1331
CviAII CATG 7 cut(s) 119, 614, 743, 918, 1030, 1189, 1238
CviQI GTAC 3 cut(s) 200, 829, 1539
DdeI CTNAG 7 cut(s) 100, 108, 136, 413, 594, 1386, 1467
DraI TTTAAA 1 cut(s) 937
Eam1104I CTCTTC 1 cut(s) 1219
EarI CTCTTC 1 cut(s) 1219
Eco130I CCWWGG 2 cut(s) 510, 1163
Eco147I AGGCCT 1 cut(s) 1060
Eco47I GGWCC 3 cut(s) 722, 893, 1151
Eco88I CYCGRG 1 cut(s) 272
EcoNI CCTNNNNNAGG 2 cut(s) 1065, 1224
EcoRII CCWGG 2 cut(s) 895, 1219
EcoT14I CCWWGG 2 cut(s) 510, 1163
ErhI CCWWGG 2 cut(s) 510, 1163
FaeI CATG 7 cut(s) 122, 617, 746, 921, 1033, 1192, 1241
FaqI GGGAC 3 cut(s) 778, 1307, 1406
FatI CATG 7 cut(s) 118, 613, 742, 917, 1029, 1188, 1237
FauI CCCGC 2 cut(s) 636, 990
FauNDI CATATG 1 cut(s) 769
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 8 cut(s) 219, 249, 252, 582, 591, 1026, 1050, 1125
FokI GGATG 3 cut(s) 16, 414, 1187
Fsp4HI GCNGC 8 cut(s) 219, 249, 252, 582, 591, 1026, 1050, 1125
FspBI CTAG 5 cut(s) 719, 1128, 1164, 1361, 1445
GluI GCNGC 8 cut(s) 219, 249, 252, 582, 591, 1026, 1050, 1125
GsaI CCCAGC 1 cut(s) 1413
GsuI CTGGAG 3 cut(s) 768, 918, 1513
HaeIII GGCC 3 cut(s) 804, 984, 1060
HapII CCGG 2 cut(s) 238, 1332
Hin1II CATG 7 cut(s) 122, 617, 746, 921, 1033, 1192, 1241
HinfI GANTC 2 cut(s) 83, 1382
HpaII CCGG 2 cut(s) 238, 1332
HphI GGTGA 5 cut(s) 107, 299, 677, 743, 1254
Hpy166II GTNNAC 3 cut(s) 310, 829, 1232
Hpy188I TCNGA 9 cut(s) 139, 156, 166, 700, 816, 823, 839, 1274, 1389
Hpy188III TCNNGA 8 cut(s) 386, 671, 785, 851, 1344, 1361, 1439, 1445
Hpy8I GTNNAC 3 cut(s) 310, 829, 1232
HpyCH4III ACNGT 4 cut(s) 458, 779, 833, 1139
HpyCH4IV ACGT 2 cut(s) 304, 1456
HpyF10VI GCNNNNNNNGC 8 cut(s) 18, 215, 327, 587, 590, 715, 1025, 1415
HpyF3I CTNAG 7 cut(s) 100, 108, 136, 413, 594, 1386, 1467
HpySE526I ACGT 2 cut(s) 304, 1456
Hsp92II CATG 7 cut(s) 122, 617, 746, 921, 1033, 1192, 1241
LmnI GCTCC 3 cut(s) 241, 787, 1042
Lsp1109I GCAGC 8 cut(s) 205, 238, 260, 593, 602, 1037, 1061, 1136
MaeI CTAG 5 cut(s) 719, 1128, 1164, 1361, 1445
MaeII ACGT 2 cut(s) 304, 1456
MaeIII GTNAC 6 cut(s) 121, 731, 1078, 1133, 1139, 1334
MboII GAAGA 3 cut(s) 586, 689, 1236
MflI RGATCY 2 cut(s) 506, 1282
MhlI GDGCHC 3 cut(s) 332, 784, 925
MluCI AATT 5 cut(s) 262, 389, 442, 692, 1313
MluI ACGCGT 1 cut(s) 752
MmeI TCCRAC 3 cut(s) 57, 560, 684
MnlI CCTC 8 cut(s) 34, 394, 508, 563, 667, 1220, 1383, 1462
MroXI GAANNNNTTC 1 cut(s) 681
MseI TTAA 4 cut(s) 90, 267, 936, 1064
MslI CAYNNNNRTG 2 cut(s) 755, 1173
MspA1I CMGCKG 2 cut(s) 251, 584
MspI CCGG 2 cut(s) 238, 1332
MspR9I CCNGG 3 cut(s) 239, 897, 1221
MvaI CCWGG 2 cut(s) 897, 1221
MvnI CGCG 2 cut(s) 754, 1432
MwoI GCNNNNNNNGC 8 cut(s) 18, 215, 327, 587, 590, 715, 1025, 1415
NciI CCSGG 1 cut(s) 239
NdeI CATATG 1 cut(s) 769
NlaIII CATG 7 cut(s) 122, 617, 746, 921, 1033, 1192, 1241
NlaIV GGNNCC 3 cut(s) 180, 508, 549
NmuCI GTSAC 3 cut(s) 731, 1078, 1133
NspI RCATGY 1 cut(s) 746
OliI CACNNNNGTG 1 cut(s) 755
PceI AGGCCT 1 cut(s) 1060
PciI ACATGT 1 cut(s) 742
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 2 cut(s) 83, 1382
PfoI TCCNGGA 2 cut(s) 237, 895
PinAI ACCGGT 1 cut(s) 1331
PkrI GCNGC 8 cut(s) 220, 250, 253, 583, 592, 1027, 1051, 1126
PscI ACATGT 1 cut(s) 742
PsiI TTATAA 1 cut(s) 860
Psp6I CCWGG 2 cut(s) 895, 1219
PspFI CCCAGC 1 cut(s) 1409
PspGI CCWGG 2 cut(s) 895, 1219
PspN4I GGNNCC 3 cut(s) 180, 508, 549
PspPI GGNCC 4 cut(s) 722, 893, 982, 1151
PsuI RGATCY 2 cut(s) 506, 1282
PvuII CAGCTG 2 cut(s) 251, 584
RsaI GTAC 3 cut(s) 201, 830, 1540
RsaNI GTAC 3 cut(s) 200, 829, 1539
RseI CAYNNNNRTG 2 cut(s) 755, 1173
SaqAI TTAA 4 cut(s) 90, 267, 936, 1064
SatI GCNGC 8 cut(s) 219, 249, 252, 582, 591, 1026, 1050, 1125
Sau96I GGNCC 4 cut(s) 722, 893, 982, 1151
ScrFI CCNGG 3 cut(s) 239, 897, 1221
SduI GDGCHC 3 cut(s) 332, 784, 925
SfcI CTRYAG 2 cut(s) 204, 977
SinI GGWCC 3 cut(s) 722, 893, 1151
SmiMI CAYNNNNRTG 2 cut(s) 755, 1173
Sse9I AATT 5 cut(s) 262, 389, 442, 692, 1313
SseBI AGGCCT 1 cut(s) 1060
SsiI CCGC 4 cut(s) 629, 997, 1430, 1460
SspI AATATT 1 cut(s) 608
SspMI CTAG 5 cut(s) 719, 1128, 1164, 1361, 1445
StuI AGGCCT 1 cut(s) 1060
StyD4I CCNGG 3 cut(s) 237, 895, 1219
StyI CCWWGG 2 cut(s) 510, 1163
TaaI ACNGT 4 cut(s) 458, 779, 833, 1139
TaiI ACGT 2 cut(s) 307, 1459
TaqI TCGA 4 cut(s) 385, 555, 852, 1180
TasI AATT 5 cut(s) 262, 389, 442, 692, 1313
TatI WGTACW 1 cut(s) 828
TfiI GAWTC 2 cut(s) 83, 1382
Tru1I TTAA 4 cut(s) 90, 267, 936, 1064
Tru9I TTAA 4 cut(s) 90, 267, 936, 1064
TscAI CASTG 1 cut(s) 354
TseFI GTSAC 3 cut(s) 731, 1078, 1133
TseI GCWGC 8 cut(s) 218, 248, 251, 581, 590, 1025, 1049, 1124
Tsp45I GTSAC 3 cut(s) 731, 1078, 1133
TspDTI ATGAA 3 cut(s) 587, 690, 1239
TspRI CASTG 1 cut(s) 354
VpaK11BI GGWCC 3 cut(s) 722, 893, 1151
XagI CCTNNNNNAGG 2 cut(s) 1065, 1224
XapI RAATTY 2 cut(s) 262, 389
XbaI TCTAGA 2 cut(s) 1360, 1444
XceI RCATGY 1 cut(s) 746
XcmI CCANNNNNNNNNTGG 2 cut(s) 757, 1294
XmaJI CCTAGG 1 cut(s) 1163
XmiI GTMKAC 1 cut(s) 309
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 5 cut(s) 719, 1128, 1164, 1361, 1445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.