Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0013098

Physical Location & Seq

Genomic Coordinates Reverse (-)

55408014 .. 55420930

Gene Structure 12917 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00374350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1725 bp

ATGACTCCCTCTTATTGCTTATCTTCAAGAAACGAAGCATTTCTTCTCCAGAACTCTCATCTCTACCTCCCAAAACACAGACCTAGTGAATCCCAGTACTTCCCAAAAAAGGAAACAAACTTTCTCAAACCCAAGTCTTCTTTCTTCCCCATCTTGAGGCTAAACAACTCCAAACTCGCACACCCTTTTGCCTTATCTTCATTCGCAGAAGCCGGAGGCGAAGAACACGAACAACAAGATGTTCAACTGAATCAACAAACCTCAAAAACCGATGACGAGGCCGAGGAGCGTGGAATGGCTGAGGCCTTCCACATATCTTCTAGAACTGCTGCGGCCATTTCCATATGCATAGCATTCGCTGCCCTTACTCTTCCCTGCTTCATGAAGTCTTTAGGACAAGGTCTGGCCTTGAAGACCAAGCTGTTGTCGTACGTAACCCTCCTGTTTGGGTTCTACATGGCTTGGAATATCGGGGCCAATGACGTGGCAAATGCCATGGGGACTTCGGTGGGCTCAGGAGCGTTGACTCTCCGGCAGGCCGTGGTGACTGCCGCGGTGTTGGAATTCTCGGGGGCCCTTCTGATGGGGACTCATGTCACGAGCACAATGCAGAAGGGGATTCTGGTGGCAAATGTGTTTCAGGGGAAAGATACTCTGCTTTTTGCTGGCTTGCTTTCTTCTCTTGCTGCTGCTGGTACTTGGTTGCAGGTTGCATCATATTATGGCTGGCCTGTGTCAACAACGCACTGTATAGTAGGATCCATGGTTGGATTTGGACTAGTCTATGGCGGAGCAGGTGCCGTCTTCTGGAGTTCACTGGCAAGAGTCATTTCTTCATGGGTTATCTCACCATTCATGGGAGCACTAGTCTCCTTTCTCGTATACAAATGCATCCGTAGGTTTGTGTATAGTGCTCCAAATCCTGGACAAGCTGCAGCTGCGGCAGCACCAATTGCTGTGCTTGTGGGTGTAAGTGCAATCTCATTTGCAGTGCTTCCCCTTGGATCAAGCTTGCCTTTGGCTGTGGCCAAGGCTCTAGGCTGTGGAGCTGCCGGTGCGCTCCTTGTGTCCAGAATTATCCAAGGGCAGCTTGGTGATCTCCTTCTCAAGTCGAAATCATCATCGCAAACTGAACCTACAGATGTCCAGAAAGATATCGGGTTTCTCTCTGATATTGCAGGTCCAACTGGTACCCAACTGAAAATAGTATACGGGGTGTTTGGATACATGCAAGTACTCTCCGCCTGCTTCATGTCATTTGCTCATGGTGGGAATGACGTCTCCAATGCAATAGGTCCTTTGGCTGGTGCATTATCTATTCTTCAAGGCGGTGCTACTGGAGGTGACATTGTTATTCCAACCGATGTTCTTGCGTGGGGAGGATTTGGAATCGTAGCTGGTCTTACAATCTGGGGATATAGGGTTATAGCGACAATTGGGAAGAAGATCACAGAACTGACACCAACTAGAGGATTTGCAGCTGAGTTTGCAGCAGCCTCCGTGGTTCTGTTTGCGTCAAAGCTGGGACTACCCATCTCTGCAACTCATACTCTAGTGGGTGCAGTAATGGGGGTGGGATTTGCAAGGGGCTTAACAGCGTTAGAGCAGAAACTGGTGGAAATGAAGCTTTTATCAGATTATGTGCAGTGGATAGCTGGATGCGAATTTGGCATAGCTTATGGGAGGATCTTGCAGAAGCTGATCAAATATATGAAAAGCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005315 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006817 GO:0006820 GO:0008150 GO:0008509 GO:0009507 GO:0009526 GO:0009536 GO:0009673 GO:0009941 GO:0015075 GO:0015103 GO:0015114 GO:0015291 GO:0015318 GO:0015698 GO:0016020 GO:0016021 GO:0022804 GO:0022857 GO:0031224 GO:0031226 GO:0031967 GO:0031975 GO:0034220 GO:0035435 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944 GO:0098656 GO:0098660 GO:0098661

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PHO4

Protein Analysis

574

Amino Acids

60.38

Weight (kDa)

9.01

Isoelectric Point (pI)

26.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PHO4	PF01384	157 - 540	5.1e-101	Phosphate transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013098)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26570 AT3G26570
fragaria_vesca	FvH4_4g25560
malus_domestica	MD13G1101400.v1.1 MD16G1102200.v1.1
prunus_persica	Prupe.1G244400_v2.0.a1
pyrus_communis	pycom13g08850 pycom16g08650
rosa_chinensis	RchiOBHm_Chr4g0432971
rosa_laevigata	RLG00000006796
rosa_multiflora	Rmu_sc0001762.1_g000044
rosa_roxburghii	Rroxscaffold_5G00374350
rosa_rugosa	Rorug04G0264500
rosa_samantha	Rh4AG318400 Rh4BG326500 Rh4CG341800 Rh4DG321700
rosa_wichuraiana	Rw4G027680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	785
AatII	GACGTC	1 cut(s)	1281
Acc36I	ACCTGC	3 cut(s)	697, 785, 1169
Acc65I	GGTACC	1 cut(s)	1190
AccB1I	GGYRCC	2 cut(s)	797, 1190
AccB7I	CCANNNNNTGG	1 cut(s)	923
AccI	GTMKAC	2 cut(s)	882, 1209
AccII	CGCG	1 cut(s)	554
AciI	CCGC	7 cut(s)	332, 552, 554, 789, 941, 1242, 1329
AclWI	GGATC	4 cut(s)	753, 766, 1012, 1694
AcoI	YGGCCR	2 cut(s)	333, 1026
AcsI	RAATTY	2 cut(s)	563, 1664
AcyI	GRCGYC	1 cut(s)	1278
AfaI	GTAC	5 cut(s)	98, 431, 697, 1192, 1236
AfiI	CCNNNNNNNGG	8 cut(s)	109, 156, 583, 807, 857, 923, 1304, 1469
AgsI	TTSAA	4 cut(s)	27, 245, 412, 1325
AhlI	ACTAGT	2 cut(s)	778, 865
AjiI	CACGTC	1 cut(s)	484
AjnI	CCWGG	1 cut(s)	922
AjuI	GAANNNNNNNTTGG	2 cut(s)	125, 157
Alw21I	GWGCWC	3 cut(s)	605, 865, 916
Alw26I	GTCTC	2 cut(s)	874, 1285
AlwI	GGATC	4 cut(s)	753, 766, 1012, 1694
AlwNI	CAGNNNCTG	2 cut(s)	1612, 1699
Ama87I	CYCGRG	1 cut(s)	568
AoxI	GGCC	9 cut(s)	279, 303, 333, 405, 474, 537, 573, 728, 1026
ApaI	GGGCCC	1 cut(s)	577
ApoI	RAATTY	2 cut(s)	563, 1664
Asp700I	GAANNNNTTC	1 cut(s)	39
Asp718I	GGTACC	1 cut(s)	1190
AspLEI	GCGC	1 cut(s)	1060
AspS9I	GGNCC	5 cut(s)	474, 573, 574, 1181, 1295
AsuHPI	GGTGA	4 cut(s)	556, 840, 1106, 1355
AvaI	CYCGRG	1 cut(s)	568
AvaII	GGWCC	2 cut(s)	1181, 1295
BaeGI	GKGCMC	1 cut(s)	577
BalI	TGGCCA	1 cut(s)	1028
BamHI	GGATCC	1 cut(s)	758
BanI	GGYRCC	2 cut(s)	797, 1190
BanII	GRGCYC	2 cut(s)	515, 577
BauI	CACGAG	1 cut(s)	598
BbsI	GAAGAC	3 cut(s)	129, 419, 796
Bbv12I	GWGCWC	3 cut(s)	605, 865, 916
BbvCI	CCTCAGC	1 cut(s)	300
BccI	CCATC	3 cut(s)	158, 577, 1541
BceAI	ACGGC	2 cut(s)	524, 785
BcgI	CGANNNNNNTGC	6 cut(s)	337, 371, 589, 623, 1352, 1386
BciT130I	CCWGG	1 cut(s)	924
BciVI	GTATCC	1 cut(s)	1217
BclI	TGATCA	1 cut(s)	1701
BcoDI	GTCTC	2 cut(s)	874, 1285
BcuI	ACTAGT	2 cut(s)	778, 865
BfaI	CTAG	7 cut(s)	84, 321, 779, 866, 1037, 1467, 1553
BfmI	CTRYAG	2 cut(s)	933, 1137
BfuAI	ACCTGC	3 cut(s)	697, 785, 1169
BfuI	GTATCC	1 cut(s)	1217
BmcAI	AGTACT	2 cut(s)	98, 1236
Bme1390I	CCNGG	1 cut(s)	924
Bme18I	GGWCC	2 cut(s)	1181, 1295
BmeT110I	CYCGRG	1 cut(s)	568
BmgBI	CACGTC	1 cut(s)	484
BmgT120I	GGNCC	5 cut(s)	474, 573, 574, 1181, 1295
BmiI	GGNNCC	6 cut(s)	475, 574, 575, 760, 799, 1192
BmrFI	CCNGG	1 cut(s)	924
BmrI	ACTGGG	1 cut(s)	88
BmsI	GCATC	3 cut(s)	722, 900, 1649
BmuI	ACTGGG	1 cut(s)	88
BoxI	GACNNNNGTC	1 cut(s)	593
BpiI	GAAGAC	3 cut(s)	129, 419, 796
BpmI	CTGGAG	3 cut(s)	32, 829, 1359
Bpu10I	CCTNAGC	2 cut(s)	300, 514
BpuEI	CTTGAG	2 cut(s)	175, 1091
BsaAI	YACGTR	1 cut(s)	433
BsaHI	GRCGYC	1 cut(s)	1278
BsaJI	CCNNGG	9 cut(s)	282, 495, 540, 552, 762, 1000, 1029, 1081, 1500
BsaXI	ACNNNNNCTCC	2 cut(s)	852, 882
Bsc4I	CCNNNNNNNGG	8 cut(s)	109, 156, 583, 807, 857, 923, 1304, 1469
Bse118I	RCCGGY	1 cut(s)	1052
Bse1I	ACTGG	5 cut(s)	94, 822, 1192, 1342, 1617
BseBI	CCWGG	1 cut(s)	924
BseDI	CCNNGG	9 cut(s)	282, 495, 540, 552, 762, 1000, 1029, 1081, 1500
BseGI	GGATG	2 cut(s)	891, 1664
BseLI	CCNNNNNNNGG	8 cut(s)	109, 156, 583, 807, 857, 923, 1304, 1469
BseMII	CTCAG	3 cut(s)	291, 528, 1473
BseNI	ACTGG	5 cut(s)	94, 822, 1192, 1342, 1617
BseRI	GAGGAG	1 cut(s)	299
BseSI	GKGCMC	1 cut(s)	577
BseYI	CCCAGC	1 cut(s)	1522
BsgI	GTGCAG	2 cut(s)	1581, 1664
Bsh1236I	CGCG	1 cut(s)	554
BshFI	GGCC	9 cut(s)	281, 305, 335, 407, 476, 539, 575, 730, 1028
BshNI	GGYRCC	2 cut(s)	797, 1190
BsiHKAI	GWGCWC	3 cut(s)	605, 865, 916
BsiHKCI	CYCGRG	1 cut(s)	568
BsiSI	CCGG	3 cut(s)	213, 532, 1053
BsiWI	CGTACG	1 cut(s)	429
BslFI	GGGAC	3 cut(s)	514, 601, 1539
BslI	CCNNNNNNNGG	8 cut(s)	109, 156, 583, 807, 857, 923, 1304, 1469
BsmAI	GTCTC	2 cut(s)	874, 1285
BsmBI	CGTCTC	1 cut(s)	1285
BsmFI	GGGAC	3 cut(s)	514, 601, 1539
BsmI	GAATGC	1 cut(s)	353
BsnI	GGCC	9 cut(s)	281, 305, 335, 407, 476, 539, 575, 730, 1028
BsoBI	CYCGRG	1 cut(s)	568
Bsp120I	GGGCCC	1 cut(s)	573
Bsp1286I	GDGCHC	5 cut(s)	515, 577, 605, 865, 916
Bsp143I	GATC	6 cut(s)	758, 1004, 1096, 1446, 1686, 1701
Bsp19I	CCATGG	2 cut(s)	495, 762
BspACI	CCGC	7 cut(s)	332, 552, 554, 789, 941, 1242, 1329
BspANI	GGCC	9 cut(s)	281, 305, 335, 407, 476, 539, 575, 730, 1028
BspCNI	CTCAG	3 cut(s)	292, 527, 1474
BspFNI	CGCG	1 cut(s)	554
BspHI	TCATGA	1 cut(s)	381
BspLI	GGNNCC	6 cut(s)	475, 574, 575, 760, 799, 1192
BspMAI	CTGCAG	1 cut(s)	937
BspMI	ACCTGC	3 cut(s)	697, 785, 1169
BspPI	GGATC	4 cut(s)	753, 766, 1012, 1694
BspT107I	GGYRCC	2 cut(s)	797, 1190
BsrFI	RCCGGY	1 cut(s)	1052
BsrI	ACTGG	5 cut(s)	94, 822, 1192, 1342, 1617
BssAI	RCCGGY	1 cut(s)	1052
BssECI	CCNNGG	9 cut(s)	282, 495, 540, 552, 762, 1000, 1029, 1081, 1500
BssMI	GATC	6 cut(s)	758, 1004, 1096, 1446, 1686, 1701
BssNAI	GTATAC	2 cut(s)	883, 1210
BssNI	GRCGYC	1 cut(s)	1278
BssSI	CACGAG	1 cut(s)	598
BssT1I	CCWWGG	5 cut(s)	495, 762, 1000, 1029, 1081
Bst1107I	GTATAC	2 cut(s)	883, 1210
Bst2BI	CACGAG	1 cut(s)	598
Bst2UI	CCWGG	1 cut(s)	924
Bst4CI	ACNGT	1 cut(s)	749
Bst6I	CTCTTC	1 cut(s)	375
BstACI	GRCGYC	1 cut(s)	1278
BstAPI	GCANNNNNTGC	2 cut(s)	359, 953
BstBAI	YACGTR	1 cut(s)	433
BstC8I	GCNNGC	6 cut(s)	537, 667, 671, 728, 1013, 1246
BstDEI	CTNAG	3 cut(s)	300, 514, 1482
BstDSI	CCRYGG	5 cut(s)	495, 540, 552, 762, 1500
BstF5I	GGATG	2 cut(s)	891, 1664
BstFNI	CGCG	1 cut(s)	554
BstHHI	GCGC	1 cut(s)	1060
BstKTI	GATC	6 cut(s)	761, 1007, 1099, 1449, 1689, 1704
BstMAI	GTCTC	2 cut(s)	874, 1285
BstMBI	GATC	6 cut(s)	758, 1004, 1096, 1446, 1686, 1701
BstMWI	GCNNNNNNNGC	8 cut(s)	359, 938, 941, 944, 953, 1055, 1487, 1668
BstNI	CCWGG	1 cut(s)	924
BstNSI	RCATGY	1 cut(s)	1231
BstPAI	GACNNNNGTC	1 cut(s)	593
BstSCI	CCNGG	1 cut(s)	922
BstSFI	CTRYAG	2 cut(s)	933, 1137
BstSLI	GKGCMC	1 cut(s)	577
BstSNI	TACGTA	1 cut(s)	433
BstUI	CGCG	1 cut(s)	554
BstV2I	GAAGAC	3 cut(s)	129, 419, 796
BstX2I	RGATCY	2 cut(s)	758, 1686
BstXI	CCANNNNNNTGG	1 cut(s)	484
BstYI	RGATCY	2 cut(s)	758, 1686
BstZ17I	GTATAC	2 cut(s)	883, 1210
BsuI	GTATCC	1 cut(s)	1217
BsuRI	GGCC	9 cut(s)	281, 305, 335, 407, 476, 539, 575, 730, 1028
BtgI	CCRYGG	5 cut(s)	495, 540, 552, 762, 1500
BtgZI	GCGATG	1 cut(s)	1107
BtrI	CACGTC	1 cut(s)	484
BtsCI	GGATG	2 cut(s)	891, 1664
BtsI	GCAGTG	2 cut(s)	996, 1652
BtsIMutI	CAGTG	4 cut(s)	745, 815, 996, 1652
BveI	ACCTGC	3 cut(s)	697, 785, 1169
Cac8I	GCNNGC	6 cut(s)	537, 667, 671, 728, 1013, 1246
CaiI	CAGNNNCTG	2 cut(s)	1612, 1699
CciI	TCATGA	1 cut(s)	381
CfoI	GCGC	1 cut(s)	1060
Cfr10I	RCCGGY	1 cut(s)	1052
Cfr13I	GGNCC	5 cut(s)	474, 573, 574, 1181, 1295
Cfr42I	CCGCGG	1 cut(s)	555
CseI	GACGC	1 cut(s)	1503
Csp6I	GTAC	5 cut(s)	97, 430, 696, 1191, 1235
CviQI	GTAC	5 cut(s)	97, 430, 696, 1191, 1235
DdeI	CTNAG	3 cut(s)	300, 514, 1482
DpnI	GATC	6 cut(s)	760, 1006, 1098, 1448, 1688, 1703
DpnII	GATC	6 cut(s)	758, 1004, 1096, 1446, 1686, 1701
EaeI	YGGCCR	2 cut(s)	333, 1026
Eam1104I	CTCTTC	1 cut(s)	375
EarI	CTCTTC	1 cut(s)	375
EciI	GGCGGA	2 cut(s)	804, 1231
Eco105I	TACGTA	1 cut(s)	433
Eco130I	CCWWGG	5 cut(s)	495, 762, 1000, 1029, 1081
Eco147I	AGGCCT	1 cut(s)	305
Eco24I	GRGCYC	2 cut(s)	515, 577
Eco32I	GATATC	1 cut(s)	1156
Eco47I	GGWCC	2 cut(s)	1181, 1295
Eco88I	CYCGRG	1 cut(s)	568
EcoO109I	RGGNCCY	3 cut(s)	573, 574, 1295
EcoRI	GAATTC	1 cut(s)	563
EcoRII	CCWGG	1 cut(s)	922
EcoRV	GATATC	1 cut(s)	1156
EcoT14I	CCWWGG	5 cut(s)	495, 762, 1000, 1029, 1081
EcoT22I	ATGCAT	2 cut(s)	350, 893
EcoT38I	GRGCYC	2 cut(s)	515, 577
ErhI	CCWWGG	5 cut(s)	495, 762, 1000, 1029, 1081
Esp3I	CGTCTC	1 cut(s)	1285
FalI	AAGNNNNNCTT	6 cut(s)	27, 59, 1000, 1032, 1074, 1106
FaqI	GGGAC	3 cut(s)	514, 601, 1539
FauNDI	CATATG	1 cut(s)	344
FbaI	TGATCA	1 cut(s)	1701
FblI	GTMKAC	2 cut(s)	882, 1209
FokI	GGATG	2 cut(s)	878, 1671
FriOI	GRGCYC	2 cut(s)	515, 577
FspBI	CTAG	7 cut(s)	84, 321, 779, 866, 1037, 1467, 1553
GlaI	GCGC	1 cut(s)	1059
GsaI	CCCAGC	1 cut(s)	1526
GsuI	CTGGAG	3 cut(s)	32, 829, 1359
HaeIII	GGCC	9 cut(s)	281, 305, 335, 407, 476, 539, 575, 730, 1028
HapII	CCGG	3 cut(s)	213, 532, 1053
HgaI	GACGC	1 cut(s)	1503
HhaI	GCGC	1 cut(s)	1060
Hin1I	GRCGYC	1 cut(s)	1278
Hin6I	GCGC	1 cut(s)	1058
HinP1I	GCGC	1 cut(s)	1058
HincII	GTYRAC	2 cut(s)	525, 738
HindII	GTYRAC	2 cut(s)	525, 738
HindIII	AAGCTT	2 cut(s)	1009, 1625
HinfI	GANTC	8 cut(s)	4, 89, 250, 526, 589, 619, 825, 1389
HpaII	CCGG	3 cut(s)	213, 532, 1053
HphI	GGTGA	4 cut(s)	556, 840, 1106, 1355
Hpy166II	GTNNAC	5 cut(s)	525, 738, 815, 883, 1210
Hpy188I	TCNGA	3 cut(s)	582, 1171, 1636
Hpy8I	GTNNAC	5 cut(s)	525, 738, 815, 883, 1210
HpyAV	CCTTC	4 cut(s)	316, 587, 607, 1112
HpyCH4III	ACNGT	1 cut(s)	749
HpyCH4IV	ACGT	3 cut(s)	432, 483, 1278
HpyF10VI	GCNNNNNNNGC	8 cut(s)	359, 938, 941, 944, 953, 1055, 1487, 1668
HpyF3I	CTNAG	3 cut(s)	300, 514, 1482
HpySE526I	ACGT	3 cut(s)	432, 483, 1278
Hsp92I	GRCGYC	1 cut(s)	1278
HspAI	GCGC	1 cut(s)	1058
KpnI	GGTACC	1 cut(s)	1194
Ksp22I	TGATCA	1 cut(s)	1701
KspI	CCGCGG	1 cut(s)	555
Kzo9I	GATC	6 cut(s)	758, 1004, 1096, 1446, 1686, 1701
LmnI	GCTCC	7 cut(s)	286, 518, 791, 860, 919, 1046, 1065
LweI	GCATC	3 cut(s)	722, 900, 1649
MaeI	CTAG	7 cut(s)	84, 321, 779, 866, 1037, 1467, 1553
MaeII	ACGT	3 cut(s)	432, 483, 1278
MaeIII	GTNAC	4 cut(s)	433, 544, 595, 1343
MalI	GATC	6 cut(s)	760, 1006, 1098, 1448, 1688, 1703
MboI	GATC	6 cut(s)	758, 1004, 1096, 1446, 1686, 1701
MfeI	CAATTG	2 cut(s)	951, 1434
MflI	RGATCY	2 cut(s)	758, 1686
MhlI	GDGCHC	5 cut(s)	515, 577, 605, 865, 916
MlsI	TGGCCA	1 cut(s)	1028
MluCI	AATT	5 cut(s)	563, 951, 1074, 1434, 1664
MluNI	TGGCCA	1 cut(s)	1028
MlyI	GAGTC	3 cut(s)	520, 583, 834
MmeI	TCCRAC	4 cut(s)	540, 748, 1208, 1382
Mox20I	TGGCCA	1 cut(s)	1028
Mph1103I	ATGCAT	2 cut(s)	350, 893
MroXI	GAANNNNTTC	1 cut(s)	39
MscI	TGGCCA	1 cut(s)	1028
MseI	TTAA	1 cut(s)	1592
Msp20I	TGGCCA	1 cut(s)	1028
MspA1I	CMGCKG	3 cut(s)	554, 938, 1481
MspI	CCGG	3 cut(s)	213, 532, 1053
MspR9I	CCNGG	1 cut(s)	924
MunI	CAATTG	2 cut(s)	951, 1434
Mva1269I	GAATGC	1 cut(s)	353
MvaI	CCWGG	1 cut(s)	924
MvnI	CGCG	1 cut(s)	554
MwoI	GCNNNNNNNGC	8 cut(s)	359, 938, 941, 944, 953, 1055, 1487, 1668
NcoI	CCATGG	2 cut(s)	495, 762
NdeI	CATATG	1 cut(s)	344
NdeII	GATC	6 cut(s)	758, 1004, 1096, 1446, 1686, 1701
NlaIV	GGNNCC	6 cut(s)	475, 574, 575, 760, 799, 1192
NmeAIII	GCCGAG	1 cut(s)	307
NmuCI	GTSAC	3 cut(s)	544, 595, 1343
NsiI	ATGCAT	2 cut(s)	350, 893
NspI	RCATGY	1 cut(s)	1231
PagI	TCATGA	1 cut(s)	381
PaqCI	CACCTGC	1 cut(s)	785
PceI	AGGCCT	1 cut(s)	305
PctI	GAATGC	1 cut(s)	353
PdmI	GAANNNNTTC	1 cut(s)	39
PfeI	GAWTC	4 cut(s)	89, 250, 619, 1389
Pfl23II	CGTACG	1 cut(s)	429
PflFI	GACNNNGTC	1 cut(s)	399
PflMI	CCANNNNNTGG	1 cut(s)	923
PfoI	TCCNGGA	1 cut(s)	922
PleI	GAGTC	3 cut(s)	520, 583, 833
PpsI	GAGTC	3 cut(s)	520, 583, 833
Ppu21I	YACGTR	1 cut(s)	433
PpuMI	RGGWCCY	1 cut(s)	1295
PshAI	GACNNNNGTC	1 cut(s)	593
Psp5II	RGGWCCY	1 cut(s)	1295
Psp6I	CCWGG	1 cut(s)	922
PspFI	CCCAGC	1 cut(s)	1522
PspGI	CCWGG	1 cut(s)	922
PspLI	CGTACG	1 cut(s)	429
PspN4I	GGNNCC	6 cut(s)	475, 574, 575, 760, 799, 1192
PspOMI	GGGCCC	1 cut(s)	573
PspPI	GGNCC	5 cut(s)	474, 573, 574, 1181, 1295
PspPPI	RGGWCCY	1 cut(s)	1295
PstI	CTGCAG	1 cut(s)	937
PstNI	CAGNNNCTG	2 cut(s)	1612, 1699
PsuI	RGATCY	2 cut(s)	758, 1686
PsyI	GACNNNGTC	1 cut(s)	399
PvuII	CAGCTG	2 cut(s)	938, 1481
RsaI	GTAC	5 cut(s)	98, 431, 697, 1192, 1236
RsaNI	GTAC	5 cut(s)	97, 430, 696, 1191, 1235
SacII	CCGCGG	1 cut(s)	555
SaqAI	TTAA	1 cut(s)	1592
Sau3AI	GATC	6 cut(s)	758, 1004, 1096, 1446, 1686, 1701
Sau96I	GGNCC	5 cut(s)	474, 573, 574, 1181, 1295
ScaI	AGTACT	2 cut(s)	98, 1236
SchI	GAGTC	3 cut(s)	520, 583, 834
ScrFI	CCNGG	1 cut(s)	924
SduI	GDGCHC	5 cut(s)	515, 577, 605, 865, 916
SfaNI	GCATC	3 cut(s)	722, 900, 1649
SfcI	CTRYAG	2 cut(s)	933, 1137
Sfr303I	CCGCGG	1 cut(s)	555
SgrBI	CCGCGG	1 cut(s)	555
SinI	GGWCC	2 cut(s)	1181, 1295
SmlI	CTYRAG	2 cut(s)	154, 1106
SmoI	CTYRAG	2 cut(s)	154, 1106
SnaBI	TACGTA	1 cut(s)	433
SpeI	ACTAGT	2 cut(s)	778, 865
Sse9I	AATT	5 cut(s)	563, 951, 1074, 1434, 1664
SseBI	AGGCCT	1 cut(s)	305
SsiI	CCGC	7 cut(s)	332, 552, 554, 789, 941, 1242, 1329
SspMI	CTAG	7 cut(s)	84, 321, 779, 866, 1037, 1467, 1553
StuI	AGGCCT	1 cut(s)	305
StyD4I	CCNGG	1 cut(s)	922
StyI	CCWWGG	5 cut(s)	495, 762, 1000, 1029, 1081
TaaI	ACNGT	1 cut(s)	749
TaiI	ACGT	3 cut(s)	435, 486, 1281
TaqI	TCGA	1 cut(s)	1112
TasI	AATT	5 cut(s)	563, 951, 1074, 1434, 1664
TatI	WGTACW	2 cut(s)	96, 1234
TauI	GCSGC	3 cut(s)	335, 554, 944
TfiI	GAWTC	4 cut(s)	89, 250, 619, 1389
Tru1I	TTAA	1 cut(s)	1592
Tru9I	TTAA	1 cut(s)	1592
TscAI	CASTG	4 cut(s)	752, 822, 996, 1652
TseFI	GTSAC	3 cut(s)	544, 595, 1343
Tsp45I	GTSAC	3 cut(s)	544, 595, 1343
TspDTI	ATGAA	7 cut(s)	189, 370, 398, 825, 844, 1240, 1637
TspGWI	ACGGA	2 cut(s)	884, 1489
TspRI	CASTG	4 cut(s)	752, 822, 996, 1652
Tth111I	GACNNNGTC	1 cut(s)	399
Van91I	CCANNNNNTGG	1 cut(s)	923
VpaK11BI	GGWCC	2 cut(s)	1181, 1295
XapI	RAATTY	2 cut(s)	563, 1664
XbaI	TCTAGA	1 cut(s)	320
XceI	RCATGY	1 cut(s)	1231
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1088
XmiI	GTMKAC	2 cut(s)	882, 1209
XmnI	GAANNNNTTC	1 cut(s)	39
XspI	CTAG	7 cut(s)	84, 321, 779, 866, 1037, 1467, 1553
ZraI	GACGTC	1 cut(s)	1279
ZrmI	AGTACT	2 cut(s)	98, 1236
Zsp2I	ATGCAT	2 cut(s)	350, 893

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.