Rroxscaffold_7G00157570

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0017994
Physical Location & Seq
Forward (+)
1371110 .. 1373014
1905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00157570.1

Sequence Viewer

Length: 1371 bp
ATGGAAGGCATGGGAAACTTGCTTTATGAGGCAGCACTAGAAGGCAATCTGACAACCCTAACAGATTTGCTCAACCAAGATAGACTGGCCCTTGACAGGTTCGTTGTCGATAGTTTCTCCGAGACTCCTCTGCATGTAGCAGCCATGCTTGGCCACCTCGATTTCACGAAGGAGATTTTGCGCCGGAAACCTGAACTTGCACAAGAGCTGGATTCGAAACGATCCTCGCCTCTTCACTTGGCATCGGCAAAAGGGTATCTGGGAATAGTGATAGAGCTGTTGAGGGTTAACCCTGACATGTGCCATGCATGCGATCGAGATGGAAGAAACCCCATCCACCTTGCTGCCATGAAAGGCCGGGTTGAAGTGTTGAGGGATTTGATCAGAGAGGCTCCTAGTGCTGCCAGGGCAACTGTGGACGAGGGAGGGACTGTGTTCCATTTATGCGTGAAACATAATCAGCTGGAGGCTATTAAGGTTCTGGTGCAGATTATGGATGATTACGATCAATTGGTGAATGCTAAGGATGATTATGGAATGACCATCTTGCACCTGGCAGTTGGTGACAAACAGATTGAGACAGTAAGGTTTTTGCTTATGTATGGTAGAGCAGAGGTGAATGCTGTAAATGCAAACGGCTTCACAGCACTTGACATTCTCACACAAAGTCGTAGAGATGTGAAAGATTTCGACATCTCAGAGTGCCTCCGAGGAGCTGGAGCCTTGAGAACTAGTCCACAGAGAAGCACCAACAGTAACCGCATCATGATGAACTCGCTCTCACACAGAACTACTGAATGTACAGGCGCTAATAATACACACAACTTGAGTAAGCCTGGGCAAGTGGAAAGGATTTCAAAGAGGAGAGACGCGTTGATGGTTGTAGCATCACTGATCGCAACCATGGCGTTTCAATCTGGGCTCAACCCTCCCGGTGGGCTTTGGCAAGACGACTCGCCGGATCACCAGGCAGGACAAGCAATCATGGCTTTTAAATACCCAACACAATACTCCTACTACCTCTCTATCAACACCATTGGTTTCATTGCCTCCCTAAGCATCATTTTGCTGCTGATCACACGGTTTCCATTCCACCACAGATTTTTCATGTGGCTTTTAACAGCCATTATGAACTTGGCGATCACATCCATGGCTATTACTTACGCCGAATCGATTTTTATTTTCACGCCAAAAAGTCAGGAGAGCAGTGTAGACAAAGTGATCAAGTACGGTATAATGGCGTGGTCCTTCGTCGTGACCCTTCTGTTGCTGCTGCAAACCTTGCGTCTCCTAGAAATTCTGATTCAAAAGTTCGACAAGTTAATGCCGAAAAGAAGAGTACATACTGCCTTCACAGATAGTTTGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

456

Amino Acids

51.08

Weight (kDa)

7.24

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 8 - 97 1.9e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 86 - 163 6.5e-09 Ankyrin repeats (3 copies)
Ank_5 PF13857 169 - 219 7.1e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 173 - 224 1e-05 Ankyrin repeats (3 copies)
PGG PF13962 284 - 394 1.1e-25 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017994)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g31330
rosa_chinensis RchiOBHm_Chr6g0311491
rosa_laevigata RLG00000010401
rosa_multiflora Rmu_sc0006915.1_g000014 Rmu_sc0015790.1_g000006
rosa_roxburghii Rroxscaffold_7G00157570
rosa_rugosa Rorug06G0389300
rosa_samantha Rh6AG503900 Rh6BG514500 Rh6CG520300 Rh6DG506400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1212
AccII CGCG 1 cut(s) 872
AciI CCGC 1 cut(s) 760
AclWI GGATC 2 cut(s) 216, 969
AcoI YGGCCR 1 cut(s) 151
AcsI RAATTY 1 cut(s) 1296
AfaI GTAC 3 cut(s) 802, 1229, 1341
AfiI CCNNNNNNNGG 2 cut(s) 96, 935
AflIII ACRYGT 2 cut(s) 297, 870
AgsI TTSAA 4 cut(s) 365, 858, 914, 1307
AhlI ACTAGT 1 cut(s) 731
AjnI CCWGG 4 cut(s) 404, 552, 835, 966
AluBI AGCT 4 cut(s) 208, 277, 463, 716
AluI AGCT 4 cut(s) 208, 277, 463, 716
Alw26I GTCTC 4 cut(s) 116, 572, 861, 1292
AlwI GGATC 2 cut(s) 216, 969
AoxI GGCC 3 cut(s) 87, 151, 355
ApeKI GCWGC 7 cut(s) 32, 140, 344, 401, 1069, 1270, 1273
ApoI RAATTY 1 cut(s) 1296
ArsI GACNNNNNNTTYG 2 cut(s) 1270, 1302
Asp700I GAANNNNTTC 1 cut(s) 686
AspLEI GCGC 2 cut(s) 183, 809
AspS9I GGNCC 2 cut(s) 88, 1245
AsuC2I CCSGG 2 cut(s) 359, 933
AsuHPI GGTGA 4 cut(s) 526, 575, 628, 956
AsuII TTCGAA 1 cut(s) 215
AvaII GGWCC 1 cut(s) 1245
BalI TGGCCA 1 cut(s) 153
BanII GRGCYC 1 cut(s) 924
BarI GAAGNNNNNNTAC 2 cut(s) 1327, 1359
BbvI GCAGC 7 cut(s) 44, 152, 331, 388, 1056, 1257, 1260
BccI CCATC 4 cut(s) 314, 341, 551, 871
BceAI ACGGC 1 cut(s) 652
BciT130I CCWGG 4 cut(s) 406, 554, 837, 968
BclI TGATCA 3 cut(s) 381, 1074, 1221
BcnI CCSGG 2 cut(s) 359, 933
BcoDI GTCTC 4 cut(s) 116, 572, 861, 1292
BcuI ACTAGT 1 cut(s) 731
BfaI CTAG 4 cut(s) 38, 396, 732, 1292
BfoI RGCGCY 1 cut(s) 810
BisI GCNGC 7 cut(s) 33, 141, 345, 402, 1070, 1271, 1274
BlsI GCNGC 7 cut(s) 34, 142, 346, 403, 1071, 1272, 1275
Bme1390I CCNGG 6 cut(s) 359, 406, 554, 837, 933, 968
Bme18I GGWCC 1 cut(s) 1245
BmgT120I GGNCC 2 cut(s) 88, 1245
BmiI GGNNCC 2 cut(s) 393, 721
BmrFI CCNGG 6 cut(s) 359, 406, 554, 837, 933, 968
BmsI GCATC 4 cut(s) 251, 771, 896, 1068
BpmI CTGGAG 2 cut(s) 485, 738
Bpu10I CCTNAGC 2 cut(s) 522, 1055
Bpu14I TTCGAA 1 cut(s) 215
BpuEI CTTGAG 2 cut(s) 745, 847
BpuMI CCSGG 2 cut(s) 359, 933
Bsa29I ATCGAT 1 cut(s) 1172
BsaBI GATNNNNATC 1 cut(s) 504
BsaJI CCNNGG 5 cut(s) 405, 709, 836, 903, 1149
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 935
Bse1I ACTGG 1 cut(s) 90
Bse3DI GCAATG 1 cut(s) 1044
Bse8I GATNNNNATC 1 cut(s) 504
BseBI CCWGG 4 cut(s) 406, 554, 837, 968
BseCI ATCGAT 1 cut(s) 1172
BseDI CCNNGG 5 cut(s) 405, 709, 836, 903, 1149
BseGI GGATG 4 cut(s) 333, 502, 532, 1145
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 2 cut(s) 96, 935
BseMI GCAATG 1 cut(s) 1044
BseMII CTCAG 1 cut(s) 711
BseNI ACTGG 1 cut(s) 90
BseRI GAGGAG 3 cut(s) 117, 726, 877
BseXI GCAGC 7 cut(s) 44, 152, 331, 388, 1056, 1257, 1260
BsgI GTGCAG 1 cut(s) 506
Bsh1236I CGCG 1 cut(s) 872
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 3 cut(s) 89, 153, 357
BshVI ATCGAT 1 cut(s) 1172
BsiEI CGRYCG 1 cut(s) 316
BsiSI CCGG 4 cut(s) 184, 358, 933, 959
BslFI GGGAC 1 cut(s) 442
BslI CCNNNNNNNGG 2 cut(s) 96, 935
BsmAI GTCTC 4 cut(s) 116, 572, 861, 1292
BsmBI CGTCTC 2 cut(s) 861, 1292
BsmFI GGGAC 1 cut(s) 442
BsmI GAATGC 2 cut(s) 523, 625
BsnI GGCC 3 cut(s) 89, 153, 357
Bsp119I TTCGAA 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 924
Bsp1407I TGTACA 1 cut(s) 800
Bsp143I GATC 9 cut(s) 221, 313, 381, 505, 894, 961, 1074, 1140, 1221
Bsp19I CCATGG 2 cut(s) 903, 1149
BspACI CCGC 1 cut(s) 760
BspANI GGCC 3 cut(s) 89, 153, 357
BspCNI CTCAG 1 cut(s) 710
BspDI ATCGAT 1 cut(s) 1172
BspFNI CGCG 1 cut(s) 872
BspHI TCATGA 1 cut(s) 765
BspLI GGNNCC 2 cut(s) 393, 721
BspPI GGATC 2 cut(s) 216, 969
BspT104I TTCGAA 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 1044
BsrGI TGTACA 1 cut(s) 800
BsrI ACTGG 1 cut(s) 90
BssECI CCNNGG 5 cut(s) 405, 709, 836, 903, 1149
BssMI GATC 9 cut(s) 221, 313, 381, 505, 894, 961, 1074, 1140, 1221
BssT1I CCWWGG 2 cut(s) 903, 1149
Bst2UI CCWGG 4 cut(s) 406, 554, 837, 968
Bst4CI ACNGT 6 cut(s) 415, 433, 583, 755, 1083, 1232
Bst6I CTCTTC 2 cut(s) 237, 1330
BstAPI GCANNNNNTGC 1 cut(s) 1282
BstAUI TGTACA 1 cut(s) 800
BstBI TTCGAA 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 310
BstDEI CTNAG 3 cut(s) 522, 697, 1055
BstDSI CCRYGG 2 cut(s) 903, 1149
BstF5I GGATG 4 cut(s) 333, 502, 532, 1145
BstFNI CGCG 1 cut(s) 872
BstH2I RGCGCY 1 cut(s) 810
BstHHI GCGC 2 cut(s) 183, 809
BstKTI GATC 9 cut(s) 224, 316, 384, 508, 897, 964, 1077, 1143, 1224
BstMAI GTCTC 4 cut(s) 116, 572, 861, 1292
BstMBI GATC 9 cut(s) 221, 313, 381, 505, 894, 961, 1074, 1140, 1221
BstMCI CGRYCG 1 cut(s) 316
BstMWI GCNNNNNNNGC 8 cut(s) 309, 398, 407, 629, 905, 977, 986, 1282
BstNI CCWGG 4 cut(s) 406, 554, 837, 968
BstNSI RCATGY 3 cut(s) 137, 301, 312
BstSCI CCNGG 6 cut(s) 357, 404, 552, 835, 931, 966
BstUI CGCG 1 cut(s) 872
BstV1I GCAGC 7 cut(s) 44, 152, 331, 388, 1056, 1257, 1260
Bsu15I ATCGAT 1 cut(s) 1172
BsuRI GGCC 3 cut(s) 89, 153, 357
BsuTUI ATCGAT 1 cut(s) 1172
BtgI CCRYGG 2 cut(s) 903, 1149
BtsCI GGATG 4 cut(s) 333, 502, 532, 1145
BtsI GCAGTG 1 cut(s) 1213
BtsIMutI CAGTG 2 cut(s) 890, 1213
Cac8I GCNNGC 1 cut(s) 310
CciI TCATGA 1 cut(s) 765
CfoI GCGC 2 cut(s) 183, 809
Cfr13I GGNCC 2 cut(s) 88, 1245
ClaI ATCGAT 1 cut(s) 1172
CseI GACGC 2 cut(s) 878, 1274
Csp6I GTAC 3 cut(s) 801, 1228, 1340
CviQI GTAC 3 cut(s) 801, 1228, 1340
DdeI CTNAG 3 cut(s) 522, 697, 1055
DpnI GATC 9 cut(s) 223, 315, 383, 507, 896, 963, 1076, 1142, 1223
DpnII GATC 9 cut(s) 221, 313, 381, 505, 894, 961, 1074, 1140, 1221
DraI TTTAAA 1 cut(s) 994
EaeI YGGCCR 1 cut(s) 151
Eam1104I CTCTTC 2 cut(s) 237, 1330
EarI CTCTTC 2 cut(s) 237, 1330
Eco130I CCWWGG 2 cut(s) 903, 1149
Eco24I GRGCYC 1 cut(s) 924
Eco47I GGWCC 1 cut(s) 1245
EcoRII CCWGG 4 cut(s) 404, 552, 835, 966
EcoT14I CCWWGG 2 cut(s) 903, 1149
EcoT22I ATGCAT 1 cut(s) 310
EcoT38I GRGCYC 1 cut(s) 924
ErhI CCWWGG 2 cut(s) 903, 1149
Esp3I CGTCTC 2 cut(s) 861, 1292
FaqI GGGAC 1 cut(s) 442
FbaI TGATCA 3 cut(s) 381, 1074, 1221
FblI GTMKAC 1 cut(s) 1212
Fnu4HI GCNGC 7 cut(s) 33, 141, 345, 402, 1070, 1271, 1274
FokI GGATG 4 cut(s) 320, 509, 539, 1132
FriOI GRGCYC 1 cut(s) 924
Fsp4HI GCNGC 7 cut(s) 33, 141, 345, 402, 1070, 1271, 1274
FspBI CTAG 4 cut(s) 38, 396, 732, 1292
GlaI GCGC 2 cut(s) 182, 808
GluI GCNGC 7 cut(s) 33, 141, 345, 402, 1070, 1271, 1274
GsuI CTGGAG 2 cut(s) 485, 738
HaeII RGCGCY 1 cut(s) 810
HaeIII GGCC 3 cut(s) 89, 153, 357
HapII CCGG 4 cut(s) 184, 358, 933, 959
HgaI GACGC 2 cut(s) 878, 1274
HhaI GCGC 2 cut(s) 183, 809
Hin6I GCGC 2 cut(s) 181, 807
HinP1I GCGC 2 cut(s) 181, 807
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 5 cut(s) 124, 212, 953, 1169, 1303
HpaI GTTAAC 1 cut(s) 289
HpaII CCGG 4 cut(s) 184, 358, 933, 959
HphI GGTGA 4 cut(s) 526, 575, 628, 956
Hpy166II GTNNAC 4 cut(s) 289, 418, 737, 1213
Hpy188I TCNGA 6 cut(s) 51, 121, 386, 700, 710, 1302
Hpy188III TCNNGA 5 cut(s) 166, 317, 766, 1199, 1255
Hpy8I GTNNAC 4 cut(s) 289, 418, 737, 1213
Hpy99I CGWCG 1 cut(s) 1256
HpyAV CCTTC 5 cut(s) 35, 163, 1258, 1271, 1360
HpyCH4III ACNGT 6 cut(s) 415, 433, 583, 755, 1083, 1232
HpyCH4V TGCA 7 cut(s) 133, 200, 308, 487, 550, 632, 1276
HpyF10VI GCNNNNNNNGC 8 cut(s) 309, 398, 407, 629, 905, 977, 986, 1282
HpyF3I CTNAG 3 cut(s) 522, 697, 1055
HspAI GCGC 2 cut(s) 181, 807
Ksp22I TGATCA 3 cut(s) 381, 1074, 1221
KspAI GTTAAC 1 cut(s) 289
Kzo9I GATC 9 cut(s) 221, 313, 381, 505, 894, 961, 1074, 1140, 1221
LmnI GCTCC 3 cut(s) 397, 713, 719
Lsp1109I GCAGC 7 cut(s) 44, 152, 331, 388, 1056, 1257, 1260
LweI GCATC 4 cut(s) 251, 771, 896, 1068
MaeI CTAG 4 cut(s) 38, 396, 732, 1292
MaeIII GTNAC 3 cut(s) 563, 755, 1255
MalI GATC 9 cut(s) 223, 315, 383, 507, 896, 963, 1076, 1142, 1223
MboI GATC 9 cut(s) 221, 313, 381, 505, 894, 961, 1074, 1140, 1221
MboII GAAGA 3 cut(s) 224, 336, 1347
MfeI CAATTG 1 cut(s) 509
MhlI GDGCHC 1 cut(s) 924
MlsI TGGCCA 1 cut(s) 153
MluCI AATT 2 cut(s) 509, 1296
MluI ACGCGT 1 cut(s) 870
MluNI TGGCCA 1 cut(s) 153
MlyI GAGTC 2 cut(s) 118, 947
Mox20I TGGCCA 1 cut(s) 153
Mph1103I ATGCAT 1 cut(s) 310
MroXI GAANNNNTTC 1 cut(s) 686
MscI TGGCCA 1 cut(s) 153
MseI TTAA 5 cut(s) 288, 474, 993, 1118, 1322
MslI CAYNNNNRTG 2 cut(s) 767, 1148
Msp20I TGGCCA 1 cut(s) 153
MspA1I CMGCKG 1 cut(s) 463
MspI CCGG 4 cut(s) 184, 358, 933, 959
MspR9I CCNGG 6 cut(s) 359, 406, 554, 837, 933, 968
MunI CAATTG 1 cut(s) 509
Mva1269I GAATGC 2 cut(s) 523, 625
MvaI CCWGG 4 cut(s) 406, 554, 837, 968
MvnI CGCG 1 cut(s) 872
MwoI GCNNNNNNNGC 8 cut(s) 309, 398, 407, 629, 905, 977, 986, 1282
NciI CCSGG 2 cut(s) 359, 933
NcoI CCATGG 2 cut(s) 903, 1149
NdeII GATC 9 cut(s) 221, 313, 381, 505, 894, 961, 1074, 1140, 1221
NlaIV GGNNCC 2 cut(s) 393, 721
NmuCI GTSAC 2 cut(s) 563, 1255
NsiI ATGCAT 1 cut(s) 310
NspI RCATGY 3 cut(s) 137, 301, 312
NspV TTCGAA 1 cut(s) 215
PaeI GCATGC 1 cut(s) 312
PagI TCATGA 1 cut(s) 765
PciI ACATGT 1 cut(s) 297
PctI GAATGC 2 cut(s) 523, 625
PdmI GAANNNNTTC 1 cut(s) 686
PfeI GAWTC 3 cut(s) 212, 1169, 1303
PkrI GCNGC 7 cut(s) 34, 142, 346, 403, 1071, 1272, 1275
Ple19I CGATCG 1 cut(s) 316
PleI GAGTC 2 cut(s) 118, 947
PpsI GAGTC 2 cut(s) 118, 947
PscI ACATGT 1 cut(s) 297
Psp6I CCWGG 4 cut(s) 404, 552, 835, 966
PspGI CCWGG 4 cut(s) 404, 552, 835, 966
PspN4I GGNNCC 2 cut(s) 393, 721
PspPI GGNCC 2 cut(s) 88, 1245
PvuI CGATCG 1 cut(s) 316
PvuII CAGCTG 1 cut(s) 463
RsaI GTAC 3 cut(s) 802, 1229, 1341
RsaNI GTAC 3 cut(s) 801, 1228, 1340
RseI CAYNNNNRTG 2 cut(s) 767, 1148
SaqAI TTAA 5 cut(s) 288, 474, 993, 1118, 1322
SatI GCNGC 7 cut(s) 33, 141, 345, 402, 1070, 1271, 1274
Sau3AI GATC 9 cut(s) 221, 313, 381, 505, 894, 961, 1074, 1140, 1221
Sau96I GGNCC 2 cut(s) 88, 1245
SchI GAGTC 2 cut(s) 118, 947
ScrFI CCNGG 6 cut(s) 359, 406, 554, 837, 933, 968
SduI GDGCHC 1 cut(s) 924
SfaNI GCATC 4 cut(s) 251, 771, 896, 1068
SfuI TTCGAA 1 cut(s) 215
SinI GGWCC 1 cut(s) 1245
SmiMI CAYNNNNRTG 2 cut(s) 767, 1148
SmlI CTYRAG 2 cut(s) 724, 826
SmoI CTYRAG 2 cut(s) 724, 826
SpeI ACTAGT 1 cut(s) 731
SphI GCATGC 1 cut(s) 312
Sse9I AATT 2 cut(s) 509, 1296
SsiI CCGC 1 cut(s) 760
SspMI CTAG 4 cut(s) 38, 396, 732, 1292
StyD4I CCNGG 6 cut(s) 357, 404, 552, 835, 931, 966
StyI CCWWGG 2 cut(s) 903, 1149
TaaI ACNGT 6 cut(s) 415, 433, 583, 755, 1083, 1232
TaqI TCGA 7 cut(s) 108, 159, 215, 316, 690, 1172, 1314
TasI AATT 2 cut(s) 509, 1296
TatI WGTACW 2 cut(s) 800, 1339
TfiI GAWTC 3 cut(s) 212, 1169, 1303
Tru1I TTAA 5 cut(s) 288, 474, 993, 1118, 1322
Tru9I TTAA 5 cut(s) 288, 474, 993, 1118, 1322
TscAI CASTG 2 cut(s) 897, 1213
TseFI GTSAC 2 cut(s) 563, 1255
TseI GCWGC 7 cut(s) 32, 140, 344, 401, 1069, 1270, 1273
Tsp45I GTSAC 2 cut(s) 563, 1255
TspDTI ATGAA 5 cut(s) 365, 785, 1033, 1096, 1145
TspRI CASTG 2 cut(s) 897, 1213
VpaK11BI GGWCC 1 cut(s) 1245
XapI RAATTY 1 cut(s) 1296
XceI RCATGY 3 cut(s) 137, 301, 312
XcmI CCANNNNNNNNNTGG 3 cut(s) 412, 550, 1132
XmiI GTMKAC 1 cut(s) 1212
XmnI GAANNNNTTC 1 cut(s) 686
XspI CTAG 4 cut(s) 38, 396, 732, 1292
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.