Rh6CG520300

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0017994
Physical Location & Seq
Reverse (-)
67619263 .. 67621607
2345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG520300.1

Sequence Viewer

Length: 1371 bp
ATGGAAGGCATGGGAAACTTGCTTTATGAGGCAGCACTAGAAGGCAATCTGACAACCCTAACAGATTTGCTCAACCAAGATAGACTGGCCCTTGACAGGTTCGTCGTCGATAGTTTCTCCGAGACTCCTCTGCACGTAGCAGCCATGCTTGGCCACCTCGATTTCACGAAGGAGCTTTTGCGCCGGAAACCTGAACTTGCACAAGAGCTGGATTTGAAACGATCCTCGCCTCTTCACTTGGCATCGGCAAAAGGGTATCTGGGAATAGTGAGAGAGCTGTTGAGGGTTAACCCTGAAATGTGCCATGCACGCGATCGAGATGGAAGAAACCCAATCCACCTTGCTGCCATGAAAGGCCGGGTTGGAGTGTTGAGGGATTTGGTCAGAGAGGCTCCTAGTGCTGCCAGGGCAACTGTGGACGAGGGAGGGACTGTGTTCCATTTATGCGTCAAACATAATCAGCTGGAGGCTATTAAGGTTCTGGTGGAGATTATGGATGATTACGATCAATTTATGAATGCTAAGGATGATTATGGAATGACCATCTTGCACCTAGCAGTTGGTGACAAACAGATTGAGACAGTAAGGTTTTTGCTTATGTATGGTAGAGCAGAGGTGAATGCTGTAAATGCAAACGGCTTCACGGAACTTGACATTCTCACACAAAGTCGTAGAGATGTGAAAGATTTCGACATCTCAGAGTGCCTCCGAGGAGCTGGAGCCTTAAGAACTAGTCCACAGAGAAGCACCAACAGTAACCACATCATGATGAACTTGCTCTCACACAGAACTACTGAATATACAGGCGCTAATAATACACACAACTTGAGTAAGACCGGACAAGTGGAAAGGATTTCAAAGAGGAGAGACGCGTTGATGGTTGTAGCATCACTGATCGCAACCATGGCTTTTCAATCTGGGCTCAACCCTCCCGGTGGGCTTTGGCAAGACGACTCGCCGGATCACCAGGCAGGACAAGCAATCATGGCTTTCAAATACCCAACACAATACTCCTACTACCTCTCTATCAACACCATTGGTTTCATTGCCTCCCTAAGCATCATTTTGCTGCTGATCACACGGTTTCCTTTCCACCACAGATTTTTCATGTGGCTTTTAACAGCCATTATGAACTTGGCGATCACATCCATGGCTATTACTTACGCCGAATCGATTTTCATTTTCACGCCAAAAAGTCAGGAGAGCAGTGTAAACAAAGTGATCAAGTACGGTATAATTGCGTGGTCCTTCGTCGTGACCCTTCTGTTGCTGCTGCAAACCTTGCGTCTCCTAGAAATTCTGATTCAAAAGTTCGACAAGTTAATGCCGAAAAGAAGAGTACATACTGCCTTCACAGATAGTTTGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

456

Amino Acids

51.31

Weight (kDa)

7.8

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 8 - 95 1.3e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 59 - 115 2.8e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 78 - 164 7.8e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 113 - 208 6.8e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 147 - 208 6.6e-08 Ankyrin repeats (3 copies)
PGG PF13962 284 - 394 1.1e-25 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017994)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g31330
rosa_chinensis RchiOBHm_Chr6g0311491
rosa_laevigata RLG00000010401
rosa_multiflora Rmu_sc0006915.1_g000014 Rmu_sc0015790.1_g000006
rosa_roxburghii Rroxscaffold_7G00157570
rosa_rugosa Rorug06G0389300
rosa_samantha Rh6AG503900 Rh6BG514500 Rh6CG520300 Rh6DG506400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 101
AccII CGCG 2 cut(s) 312, 872
AclWI GGATC 2 cut(s) 216, 969
AcoI YGGCCR 1 cut(s) 151
AcsI RAATTY 1 cut(s) 1296
AfaI GTAC 2 cut(s) 1229, 1341
AfiI CCNNNNNNNGG 2 cut(s) 96, 935
AflII CTTAAG 1 cut(s) 724
AflIII ACRYGT 1 cut(s) 870
AgsI TTSAA 5 cut(s) 217, 858, 914, 994, 1307
AhlI ACTAGT 1 cut(s) 731
AjnI CCWGG 2 cut(s) 404, 966
AluBI AGCT 5 cut(s) 175, 208, 277, 463, 716
AluI AGCT 5 cut(s) 175, 208, 277, 463, 716
Alw26I GTCTC 4 cut(s) 116, 572, 861, 1292
AlwI GGATC 2 cut(s) 216, 969
AoxI GGCC 3 cut(s) 87, 151, 355
ApeKI GCWGC 7 cut(s) 32, 140, 344, 401, 1069, 1270, 1273
ApoI RAATTY 1 cut(s) 1296
ArsI GACNNNNNNTTYG 2 cut(s) 1270, 1302
Asp700I GAANNNNTTC 1 cut(s) 686
AspLEI GCGC 2 cut(s) 183, 809
AspS9I GGNCC 2 cut(s) 88, 1245
AsuC2I CCSGG 2 cut(s) 359, 933
AsuHPI GGTGA 3 cut(s) 575, 628, 956
AvaII GGWCC 1 cut(s) 1245
BalI TGGCCA 1 cut(s) 153
BanII GRGCYC 1 cut(s) 924
BarI GAAGNNNNNNTAC 2 cut(s) 1327, 1359
BbvI GCAGC 7 cut(s) 44, 152, 331, 388, 1056, 1257, 1260
BccI CCATC 3 cut(s) 314, 551, 871
BceAI ACGGC 1 cut(s) 652
BciT130I CCWGG 2 cut(s) 406, 968
BclI TGATCA 2 cut(s) 1074, 1221
BcnI CCSGG 2 cut(s) 359, 933
BcoDI GTCTC 4 cut(s) 116, 572, 861, 1292
BcuI ACTAGT 1 cut(s) 731
BfaI CTAG 5 cut(s) 38, 396, 554, 732, 1292
BfoI RGCGCY 1 cut(s) 810
BfrI CTTAAG 1 cut(s) 724
BisI GCNGC 7 cut(s) 33, 141, 345, 402, 1070, 1271, 1274
BlsI GCNGC 7 cut(s) 34, 142, 346, 403, 1071, 1272, 1275
Bme1390I CCNGG 4 cut(s) 359, 406, 933, 968
Bme18I GGWCC 1 cut(s) 1245
BmgT120I GGNCC 2 cut(s) 88, 1245
BmiI GGNNCC 2 cut(s) 393, 721
BmrFI CCNGG 4 cut(s) 359, 406, 933, 968
BmsI GCATC 3 cut(s) 251, 896, 1068
BpmI CTGGAG 2 cut(s) 485, 738
Bpu10I CCTNAGC 2 cut(s) 522, 1055
BpuEI CTTGAG 1 cut(s) 847
BpuMI CCSGG 2 cut(s) 359, 933
Bsa29I ATCGAT 1 cut(s) 1172
BsaAI YACGTR 1 cut(s) 136
BsaBI GATNNNNATC 1 cut(s) 504
BsaJI CCNNGG 4 cut(s) 405, 709, 903, 1149
BsaWI WCCGGW 1 cut(s) 836
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 935
Bse1I ACTGG 1 cut(s) 90
Bse3DI GCAATG 1 cut(s) 1044
Bse8I GATNNNNATC 1 cut(s) 504
BseBI CCWGG 2 cut(s) 406, 968
BseCI ATCGAT 1 cut(s) 1172
BseDI CCNNGG 4 cut(s) 405, 709, 903, 1149
BseGI GGATG 3 cut(s) 502, 532, 1145
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 2 cut(s) 96, 935
BseMI GCAATG 1 cut(s) 1044
BseMII CTCAG 1 cut(s) 711
BseNI ACTGG 1 cut(s) 90
BseRI GAGGAG 3 cut(s) 117, 726, 877
BseXI GCAGC 7 cut(s) 44, 152, 331, 388, 1056, 1257, 1260
BsgI GTGCAG 1 cut(s) 116
Bsh1236I CGCG 2 cut(s) 312, 872
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 3 cut(s) 89, 153, 357
BshVI ATCGAT 1 cut(s) 1172
BsiEI CGRYCG 1 cut(s) 316
BsiSI CCGG 5 cut(s) 184, 358, 837, 933, 959
BslFI GGGAC 1 cut(s) 442
BslI CCNNNNNNNGG 2 cut(s) 96, 935
BsmAI GTCTC 4 cut(s) 116, 572, 861, 1292
BsmBI CGTCTC 2 cut(s) 861, 1292
BsmFI GGGAC 1 cut(s) 442
BsmI GAATGC 2 cut(s) 523, 625
BsnI GGCC 3 cut(s) 89, 153, 357
Bsp1286I GDGCHC 1 cut(s) 924
Bsp143I GATC 8 cut(s) 221, 313, 505, 894, 961, 1074, 1140, 1221
Bsp19I CCATGG 2 cut(s) 903, 1149
BspANI GGCC 3 cut(s) 89, 153, 357
BspCNI CTCAG 1 cut(s) 710
BspDI ATCGAT 1 cut(s) 1172
BspFNI CGCG 2 cut(s) 312, 872
BspHI TCATGA 1 cut(s) 765
BspLI GGNNCC 2 cut(s) 393, 721
BspPI GGATC 2 cut(s) 216, 969
BspTI CTTAAG 1 cut(s) 724
BsrDI GCAATG 1 cut(s) 1044
BsrI ACTGG 1 cut(s) 90
BssECI CCNNGG 4 cut(s) 405, 709, 903, 1149
BssMI GATC 8 cut(s) 221, 313, 505, 894, 961, 1074, 1140, 1221
BssT1I CCWWGG 2 cut(s) 903, 1149
Bst2UI CCWGG 2 cut(s) 406, 968
Bst4CI ACNGT 6 cut(s) 415, 433, 583, 755, 1083, 1232
Bst6I CTCTTC 2 cut(s) 237, 1330
BstAFI CTTAAG 1 cut(s) 724
BstAPI GCANNNNNTGC 1 cut(s) 1282
BstBAI YACGTR 1 cut(s) 136
BstC8I GCNNGC 1 cut(s) 310
BstDEI CTNAG 3 cut(s) 522, 697, 1055
BstDSI CCRYGG 2 cut(s) 903, 1149
BstF5I GGATG 3 cut(s) 502, 532, 1145
BstFNI CGCG 2 cut(s) 312, 872
BstH2I RGCGCY 1 cut(s) 810
BstHHI GCGC 2 cut(s) 183, 809
BstKTI GATC 8 cut(s) 224, 316, 508, 897, 964, 1077, 1143, 1224
BstMAI GTCTC 4 cut(s) 116, 572, 861, 1292
BstMBI GATC 8 cut(s) 221, 313, 505, 894, 961, 1074, 1140, 1221
BstMCI CGRYCG 1 cut(s) 316
BstMWI GCNNNNNNNGC 8 cut(s) 309, 398, 407, 629, 905, 977, 986, 1282
BstNI CCWGG 2 cut(s) 406, 968
BstSCI CCNGG 4 cut(s) 357, 404, 931, 966
BstUI CGCG 2 cut(s) 312, 872
BstV1I GCAGC 7 cut(s) 44, 152, 331, 388, 1056, 1257, 1260
Bsu15I ATCGAT 1 cut(s) 1172
BsuRI GGCC 3 cut(s) 89, 153, 357
BsuTUI ATCGAT 1 cut(s) 1172
BtgI CCRYGG 2 cut(s) 903, 1149
BtsCI GGATG 3 cut(s) 502, 532, 1145
BtsI GCAGTG 1 cut(s) 1213
BtsIMutI CAGTG 2 cut(s) 890, 1213
Cac8I GCNNGC 1 cut(s) 310
CciI TCATGA 1 cut(s) 765
CfoI GCGC 2 cut(s) 183, 809
Cfr13I GGNCC 2 cut(s) 88, 1245
ClaI ATCGAT 1 cut(s) 1172
CseI GACGC 3 cut(s) 436, 878, 1274
Csp6I GTAC 2 cut(s) 1228, 1340
CviAII CATG 9 cut(s) 10, 145, 305, 349, 766, 904, 985, 1108, 1150
CviQI GTAC 2 cut(s) 1228, 1340
DdeI CTNAG 3 cut(s) 522, 697, 1055
DpnI GATC 8 cut(s) 223, 315, 507, 896, 963, 1076, 1142, 1223
DpnII GATC 8 cut(s) 221, 313, 505, 894, 961, 1074, 1140, 1221
DrdI GACNNNNNNGTC 1 cut(s) 101
DseDI GACNNNNNNGTC 1 cut(s) 101
EaeI YGGCCR 1 cut(s) 151
Eam1104I CTCTTC 2 cut(s) 237, 1330
EarI CTCTTC 2 cut(s) 237, 1330
Eco130I CCWWGG 2 cut(s) 903, 1149
Eco24I GRGCYC 1 cut(s) 924
Eco47I GGWCC 1 cut(s) 1245
EcoRII CCWGG 2 cut(s) 404, 966
EcoT14I CCWWGG 2 cut(s) 903, 1149
EcoT38I GRGCYC 1 cut(s) 924
ErhI CCWWGG 2 cut(s) 903, 1149
Esp3I CGTCTC 2 cut(s) 861, 1292
FaeI CATG 9 cut(s) 13, 148, 308, 352, 769, 907, 988, 1111, 1153
FaqI GGGAC 1 cut(s) 442
FatI CATG 9 cut(s) 9, 144, 304, 348, 765, 903, 984, 1107, 1149
FbaI TGATCA 2 cut(s) 1074, 1221
Fnu4HI GCNGC 7 cut(s) 33, 141, 345, 402, 1070, 1271, 1274
FokI GGATG 3 cut(s) 509, 539, 1132
FriOI GRGCYC 1 cut(s) 924
Fsp4HI GCNGC 7 cut(s) 33, 141, 345, 402, 1070, 1271, 1274
FspBI CTAG 5 cut(s) 38, 396, 554, 732, 1292
GlaI GCGC 2 cut(s) 182, 808
GluI GCNGC 7 cut(s) 33, 141, 345, 402, 1070, 1271, 1274
GsuI CTGGAG 2 cut(s) 485, 738
HaeII RGCGCY 1 cut(s) 810
HaeIII GGCC 3 cut(s) 89, 153, 357
HapII CCGG 5 cut(s) 184, 358, 837, 933, 959
HgaI GACGC 3 cut(s) 436, 878, 1274
HhaI GCGC 2 cut(s) 183, 809
Hin1II CATG 9 cut(s) 13, 148, 308, 352, 769, 907, 988, 1111, 1153
Hin6I GCGC 2 cut(s) 181, 807
HinP1I GCGC 2 cut(s) 181, 807
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 4 cut(s) 124, 953, 1169, 1303
HpaI GTTAAC 1 cut(s) 289
HpaII CCGG 5 cut(s) 184, 358, 837, 933, 959
HphI GGTGA 3 cut(s) 575, 628, 956
Hpy166II GTNNAC 4 cut(s) 289, 418, 737, 1213
Hpy188I TCNGA 6 cut(s) 51, 121, 386, 700, 710, 1302
Hpy188III TCNNGA 5 cut(s) 166, 317, 766, 1199, 1255
Hpy8I GTNNAC 4 cut(s) 289, 418, 737, 1213
Hpy99I CGWCG 3 cut(s) 107, 110, 1256
HpyAV CCTTC 5 cut(s) 35, 163, 1258, 1271, 1360
HpyCH4III ACNGT 6 cut(s) 415, 433, 583, 755, 1083, 1232
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 6 cut(s) 133, 200, 308, 550, 632, 1276
HpyF10VI GCNNNNNNNGC 8 cut(s) 309, 398, 407, 629, 905, 977, 986, 1282
HpyF3I CTNAG 3 cut(s) 522, 697, 1055
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 9 cut(s) 13, 148, 308, 352, 769, 907, 988, 1111, 1153
HspAI GCGC 2 cut(s) 181, 807
Ksp22I TGATCA 2 cut(s) 1074, 1221
KspAI GTTAAC 1 cut(s) 289
Kzo9I GATC 8 cut(s) 221, 313, 505, 894, 961, 1074, 1140, 1221
LmnI GCTCC 4 cut(s) 172, 397, 713, 719
Lsp1109I GCAGC 7 cut(s) 44, 152, 331, 388, 1056, 1257, 1260
LweI GCATC 3 cut(s) 251, 896, 1068
MaeI CTAG 5 cut(s) 38, 396, 554, 732, 1292
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 3 cut(s) 563, 755, 1255
MalI GATC 8 cut(s) 223, 315, 507, 896, 963, 1076, 1142, 1223
MboI GATC 8 cut(s) 221, 313, 505, 894, 961, 1074, 1140, 1221
MboII GAAGA 3 cut(s) 224, 336, 1347
MhlI GDGCHC 1 cut(s) 924
MlsI TGGCCA 1 cut(s) 153
MluCI AATT 3 cut(s) 509, 1236, 1296
MluI ACGCGT 1 cut(s) 870
MluNI TGGCCA 1 cut(s) 153
MlyI GAGTC 2 cut(s) 118, 947
MmeI TCCRAC 1 cut(s) 343
Mox20I TGGCCA 1 cut(s) 153
MroXI GAANNNNTTC 1 cut(s) 686
MscI TGGCCA 1 cut(s) 153
MseI TTAA 5 cut(s) 288, 474, 725, 1118, 1322
MslI CAYNNNNRTG 2 cut(s) 767, 1148
Msp20I TGGCCA 1 cut(s) 153
MspA1I CMGCKG 1 cut(s) 463
MspCI CTTAAG 1 cut(s) 724
MspI CCGG 5 cut(s) 184, 358, 837, 933, 959
MspR9I CCNGG 4 cut(s) 359, 406, 933, 968
Mva1269I GAATGC 2 cut(s) 523, 625
MvaI CCWGG 2 cut(s) 406, 968
MvnI CGCG 2 cut(s) 312, 872
MwoI GCNNNNNNNGC 8 cut(s) 309, 398, 407, 629, 905, 977, 986, 1282
NciI CCSGG 2 cut(s) 359, 933
NcoI CCATGG 2 cut(s) 903, 1149
NdeII GATC 8 cut(s) 221, 313, 505, 894, 961, 1074, 1140, 1221
NlaIII CATG 9 cut(s) 13, 148, 308, 352, 769, 907, 988, 1111, 1153
NlaIV GGNNCC 2 cut(s) 393, 721
NmuCI GTSAC 2 cut(s) 563, 1255
PagI TCATGA 1 cut(s) 765
PctI GAATGC 2 cut(s) 523, 625
PdmI GAANNNNTTC 1 cut(s) 686
PfeI GAWTC 2 cut(s) 1169, 1303
PkrI GCNGC 7 cut(s) 34, 142, 346, 403, 1071, 1272, 1275
Ple19I CGATCG 1 cut(s) 316
PleI GAGTC 2 cut(s) 118, 947
PpsI GAGTC 2 cut(s) 118, 947
Ppu21I YACGTR 1 cut(s) 136
Psp6I CCWGG 2 cut(s) 404, 966
PspGI CCWGG 2 cut(s) 404, 966
PspN4I GGNNCC 2 cut(s) 393, 721
PspPI GGNCC 2 cut(s) 88, 1245
PvuI CGATCG 1 cut(s) 316
PvuII CAGCTG 1 cut(s) 463
RsaI GTAC 2 cut(s) 1229, 1341
RsaNI GTAC 2 cut(s) 1228, 1340
RseI CAYNNNNRTG 2 cut(s) 767, 1148
SaqAI TTAA 5 cut(s) 288, 474, 725, 1118, 1322
SatI GCNGC 7 cut(s) 33, 141, 345, 402, 1070, 1271, 1274
Sau3AI GATC 8 cut(s) 221, 313, 505, 894, 961, 1074, 1140, 1221
Sau96I GGNCC 2 cut(s) 88, 1245
SchI GAGTC 2 cut(s) 118, 947
ScrFI CCNGG 4 cut(s) 359, 406, 933, 968
SduI GDGCHC 1 cut(s) 924
SfaNI GCATC 3 cut(s) 251, 896, 1068
SinI GGWCC 1 cut(s) 1245
SmiMI CAYNNNNRTG 2 cut(s) 767, 1148
SmlI CTYRAG 2 cut(s) 724, 826
SmoI CTYRAG 2 cut(s) 724, 826
SpeI ACTAGT 1 cut(s) 731
Sse9I AATT 3 cut(s) 509, 1236, 1296
SspMI CTAG 5 cut(s) 38, 396, 554, 732, 1292
StyD4I CCNGG 4 cut(s) 357, 404, 931, 966
StyI CCWWGG 2 cut(s) 903, 1149
TaaI ACNGT 6 cut(s) 415, 433, 583, 755, 1083, 1232
TaiI ACGT 1 cut(s) 138
TaqI TCGA 6 cut(s) 108, 159, 316, 690, 1172, 1314
TasI AATT 3 cut(s) 509, 1236, 1296
TatI WGTACW 1 cut(s) 1339
TfiI GAWTC 2 cut(s) 1169, 1303
Tru1I TTAA 5 cut(s) 288, 474, 725, 1118, 1322
Tru9I TTAA 5 cut(s) 288, 474, 725, 1118, 1322
TscAI CASTG 2 cut(s) 897, 1213
TseFI GTSAC 2 cut(s) 563, 1255
TseI GCWGC 7 cut(s) 32, 140, 344, 401, 1069, 1270, 1273
Tsp45I GTSAC 2 cut(s) 563, 1255
TspDTI ATGAA 7 cut(s) 365, 530, 785, 1033, 1096, 1145, 1168
TspGWI ACGGA 1 cut(s) 659
TspRI CASTG 2 cut(s) 897, 1213
Vha464I CTTAAG 1 cut(s) 724
VpaK11BI GGWCC 1 cut(s) 1245
XapI RAATTY 1 cut(s) 1296
XcmI CCANNNNNNNNNTGG 2 cut(s) 412, 1132
XmnI GAANNNNTTC 1 cut(s) 686
XspI CTAG 5 cut(s) 38, 396, 554, 732, 1292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.