Rroxscaffold_7G00176000

HUA2-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0025528
Physical Location & Seq
Forward (+)
15469021 .. 15469953
933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00176000.1

Sequence Viewer

Length: 270 bp
ATGCCGGCGAATCCCTCCCGGAGATTTTCCACAATTGTTCAATTCCTTGGGCGATTGCGGCACATAGCTCAGTTCCGCAGGAGGAAAGGAGCTAGCAAAGCTGCTCAGGCCGCCGCGGCTCGCCGCCAGTGGAAGGTCGGCGATCTTGTTCTTGCTAAAGTCAAAGGGTTCCCTGCTTGGCCTGCTACGGTAAGTGAGCCAGAGAAGTGGGGTTACTCTGCTGATTGGAAGAAAGTACTTGTTTTCTTCTTTGGAACACAACAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CTD_bind GSHPx PWWP

Protein Analysis

89

Amino Acids

10.13

Weight (kDa)

11.73

Isoelectric Point (pI)

58.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 47 - 87 2e-10 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025528)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002226.1_g000001
rosa_roxburghii Rroxscaffold_7G00176000
rosa_samantha Rh2BG253800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 116
AciI CCGC 6 cut(s) 58, 76, 111, 114, 116, 124
AfaI GTAC 1 cut(s) 237
AfiI CCNNNNNNNGG 1 cut(s) 133
AgsI TTSAA 1 cut(s) 41
AluBI AGCT 3 cut(s) 68, 92, 101
AluI AGCT 3 cut(s) 68, 92, 101
AoxI GGCC 2 cut(s) 108, 179
ApeKI GCWGC 1 cut(s) 101
AsuC2I CCSGG 1 cut(s) 19
AsuNHI GCTAGC 1 cut(s) 92
BarI GAAGNNNNNNTAC 2 cut(s) 197, 229
BbvI GCAGC 1 cut(s) 88
BcnI CCSGG 1 cut(s) 19
BfaI CTAG 1 cut(s) 93
BglI GCCNNNNNGGC 1 cut(s) 116
BisI GCNGC 6 cut(s) 59, 102, 111, 114, 117, 124
BlsI GCNGC 6 cut(s) 60, 103, 112, 115, 118, 125
BmcAI AGTACT 1 cut(s) 237
Bme1390I CCNGG 1 cut(s) 19
BmiI GGNNCC 1 cut(s) 170
BmrFI CCNGG 1 cut(s) 19
BmtI GCTAGC 1 cut(s) 96
Bpu10I CCTNAGC 1 cut(s) 105
BpuMI CCSGG 1 cut(s) 19
BsaJI CCNNGG 2 cut(s) 46, 114
Bsc4I CCNNNNNNNGG 1 cut(s) 133
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 1 cut(s) 127
BseDI CCNNGG 2 cut(s) 46, 114
BseLI CCNNNNNNNGG 1 cut(s) 133
BseMII CTCAG 2 cut(s) 83, 119
BseNI ACTGG 1 cut(s) 127
BseXI GCAGC 1 cut(s) 88
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 2 cut(s) 110, 181
BsiSI CCGG 2 cut(s) 5, 19
BslI CCNNNNNNNGG 1 cut(s) 133
BsnI GGCC 2 cut(s) 110, 181
Bsp143I GATC 1 cut(s) 142
BspACI CCGC 6 cut(s) 58, 76, 111, 114, 116, 124
BspANI GGCC 2 cut(s) 110, 181
BspCNI CTCAG 2 cut(s) 82, 118
BspFNI CGCG 1 cut(s) 116
BspLI GGNNCC 1 cut(s) 170
BspOI GCTAGC 1 cut(s) 96
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 1 cut(s) 127
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 2 cut(s) 46, 114
BssMI GATC 1 cut(s) 142
BssT1I CCWWGG 1 cut(s) 46
Bst4CI ACNGT 1 cut(s) 190
BstC8I GCNNGC 4 cut(s) 6, 94, 121, 183
BstDEI CTNAG 2 cut(s) 69, 105
BstDSI CCRYGG 1 cut(s) 114
BstFNI CGCG 1 cut(s) 116
BstKTI GATC 1 cut(s) 145
BstMBI GATC 1 cut(s) 142
BstMWI GCNNNNNNNGC 6 cut(s) 58, 98, 107, 110, 116, 182
BstSCI CCNGG 1 cut(s) 17
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 1 cut(s) 88
BstXI CCANNNNNNTGG 1 cut(s) 207
BsuRI GGCC 2 cut(s) 110, 181
BtgI CCRYGG 1 cut(s) 114
BtsIMutI CAGTG 1 cut(s) 134
Cac8I GCNNGC 4 cut(s) 6, 94, 121, 183
Cfr10I RCCGGY 1 cut(s) 4
Cfr42I CCGCGG 1 cut(s) 117
Csp6I GTAC 1 cut(s) 236
CviJI RGCY 7 cut(s) 68, 92, 101, 110, 119, 181, 199
CviKI_1 RGCY 7 cut(s) 68, 92, 101, 110, 119, 181, 199
CviQI GTAC 1 cut(s) 236
DdeI CTNAG 2 cut(s) 69, 105
DpnI GATC 1 cut(s) 144
DpnII GATC 1 cut(s) 142
Eco130I CCWWGG 1 cut(s) 46
EcoT14I CCWWGG 1 cut(s) 46
ErhI CCWWGG 1 cut(s) 46
FaiI YATR 1 cut(s) 65
Fnu4HI GCNGC 6 cut(s) 59, 102, 111, 114, 117, 124
Fsp4HI GCNGC 6 cut(s) 59, 102, 111, 114, 117, 124
FspBI CTAG 1 cut(s) 93
GluI GCNGC 6 cut(s) 59, 102, 111, 114, 117, 124
HaeIII GGCC 2 cut(s) 110, 181
HapII CCGG 2 cut(s) 5, 19
HinfI GANTC 1 cut(s) 10
HpaII CCGG 2 cut(s) 5, 19
HpyAV CCTTC 1 cut(s) 127
HpyCH4III ACNGT 1 cut(s) 190
HpyF10VI GCNNNNNNNGC 6 cut(s) 58, 98, 107, 110, 116, 182
HpyF3I CTNAG 2 cut(s) 69, 105
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
KspI CCGCGG 1 cut(s) 117
Kzo9I GATC 1 cut(s) 142
LmnI GCTCC 1 cut(s) 89
LpnPI CCDG 8 cut(s) 18, 32, 64, 92, 140, 186, 195, 213
Lsp1109I GCAGC 1 cut(s) 88
MaeI CTAG 1 cut(s) 93
MaeIII GTNAC 1 cut(s) 212
MalI GATC 1 cut(s) 144
MboI GATC 1 cut(s) 142
MboII GAAGA 2 cut(s) 238, 241
MfeI CAATTG 1 cut(s) 33
MluCI AATT 2 cut(s) 33, 41
MnlI CCTC 2 cut(s) 25, 75
MroNI GCCGGC 1 cut(s) 4
MspA1I CMGCKG 1 cut(s) 116
MspI CCGG 2 cut(s) 5, 19
MspR9I CCNGG 1 cut(s) 19
MunI CAATTG 1 cut(s) 33
MvnI CGCG 1 cut(s) 116
MwoI GCNNNNNNNGC 6 cut(s) 58, 98, 107, 110, 116, 182
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 1 cut(s) 19
NdeII GATC 1 cut(s) 142
NgoMIV GCCGGC 1 cut(s) 4
NheI GCTAGC 1 cut(s) 92
NlaIV GGNNCC 1 cut(s) 170
PdiI GCCGGC 1 cut(s) 6
PfeI GAWTC 1 cut(s) 10
PfoI TCCNGGA 1 cut(s) 17
PkrI GCNGC 6 cut(s) 60, 103, 112, 115, 118, 125
PspN4I GGNNCC 1 cut(s) 170
RsaI GTAC 1 cut(s) 237
RsaNI GTAC 1 cut(s) 236
SacII CCGCGG 1 cut(s) 117
SatI GCNGC 6 cut(s) 59, 102, 111, 114, 117, 124
Sau3AI GATC 1 cut(s) 142
ScaI AGTACT 1 cut(s) 237
ScrFI CCNGG 1 cut(s) 19
SetI ASST 4 cut(s) 70, 94, 103, 138
Sfr303I CCGCGG 1 cut(s) 117
SgrBI CCGCGG 1 cut(s) 117
Sse9I AATT 2 cut(s) 33, 41
SsiI CCGC 6 cut(s) 58, 76, 111, 114, 116, 124
SspMI CTAG 1 cut(s) 93
StyD4I CCNGG 1 cut(s) 17
StyI CCWWGG 1 cut(s) 46
TaaI ACNGT 1 cut(s) 190
TasI AATT 2 cut(s) 33, 41
TatI WGTACW 1 cut(s) 235
TauI GCSGC 5 cut(s) 61, 113, 116, 119, 126
TfiI GAWTC 1 cut(s) 10
TscAI CASTG 1 cut(s) 134
TseI GCWGC 1 cut(s) 101
TspRI CASTG 1 cut(s) 134
XspI CTAG 1 cut(s) 93
ZrmI AGTACT 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.