Rroxscaffold_7G00195530

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0015537
Physical Location & Seq
Reverse (-)
37952636 .. 37955893
3258 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00195530.1

Sequence Viewer

Length: 1236 bp
ATGGACGAGGCTCTCAAATATGGTGGATTAACAACATTGTCGGATTCTTATGCTATCAATGGCCAGCCAGGAGATTTTTACGAATGTTCCAACGAAACAACATTTCGATTGGCGGTTGATCATGGGAGTACCTATCTTCTCCGTTTAGTGAATTCGGTACAAAACACAGACATGTTCTTCGCCATAGCAGATCATAATCTCACACTTGTGGGCGTGGATGGTAACTACGTCAAACCTGTAGTTACCAACTACCTAATGATAACCCCAGGAGAAACAATGGACATTTTGGTCACAGCAACCCAACCTCCTGGCCATTACTACATGCTTATAAGCCCTTATTTCGATGGACAAGCTGATGACTTCGACAAAAGTGTCACCAGCGCAATATTTCAGTACAACGGCAACTACACTCCTACGTCATCACCCATCTACCAAGACCACATTCCTGGCTTCTATGACGTTGACGCTGCACGCAACTTTGACAGACAGTTGAAGAGTTTGGCATCACCGGAGCACCCTGCAGACGTTCCTCAACATATTACCACCAGGATGTTTATTTCCCTTTCCATTAGCATGCTTCGTTGTCCCAACGACACATGTGATGGACCTGATGGAAATCGGCTTGCATCTGCAATGAACAACATCAGCTTTGCCAACCCTTCCATTGATGTGCTGCAAGCATACTACAGGAACATAACTGGATATTTCGAAGCAGATTTCCCAGACAAGCCGCCTACTCTATTCAACTTCACCTCAGATGATTTGATGACTGACAACGTTACGTTAAGTGACCAAGGAACCAGGGTGAAGATGTTGAATTACAACGAGACTGTGGAAATAGTATTCCAAGGGACAAATGTCATGAACTCAGGAGAGAATCATCCCGTGCATTTGCATGGGTTCAAGTTTTACGTGGTTGGAGCTGGTGTTGGGAATTTCGACAATGTCACTGATCCCTTAACTTACAATTTGATTGATCCACCACAAGTTAATACCTTCCCAGTTCCTAAGGATGGATGGGCCACCATCAGATTCGTAGCCGACAACCCAGGGGTATGGTTCATGCACTGCCATTACGATCGGCATATGAGTTGGGGCATGGATACCGCTTTCATAGTGCGGAATGGTGACACAGAGGAGACGAGTGTTCGCCCTCCACCTGATTACATGCCGTCTTGTGGCGAAAATTCCATATACGGTGGTGCTGAGCAGTCAATGTTTCAGTTGGAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

411

Amino Acids

45.91

Weight (kDa)

4.45

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase PF00394 8 - 135 1.1e-33 Multicopper oxidase
Cu-oxidase_2 PF07731 239 - 376 7.3e-41 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015537)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12620 FvH4_2g12620 FvH4_2g12620
malus_domestica MD00G1014900.v1.1
prunus_persica Prupe.8G047000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0273241
rosa_laevigata RLG00000013628
rosa_multiflora Rmu_sc0000239.1_g000030
rosa_roxburghii Rroxscaffold_7G00195530 Rroxscaffold_7G00195540
rosa_rugosa Rorug06G0073700
rosa_samantha Rh6AG189200 Rh6BG192400 Rh6DG183200
rosa_wichuraiana Rw6G016400 Rw6G016460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 329
AasI GACNNNNNNGTC 2 cut(s) 287, 371
AciI CCGC 4 cut(s) 113, 731, 1107, 1120
AclI AACGTT 1 cut(s) 777
AclWI GGATC 2 cut(s) 947, 971
AcoI YGGCCR 2 cut(s) 61, 310
AcsI RAATTY 3 cut(s) 151, 934, 1186
AfaI GTAC 3 cut(s) 130, 159, 395
AfiI CCNNNNNNNGG 2 cut(s) 1013, 1178
AflIII ACRYGT 2 cut(s) 171, 596
AgsI TTSAA 4 cut(s) 493, 745, 817, 904
AjnI CCWGG 7 cut(s) 67, 265, 307, 445, 545, 800, 1048
AjuI GAANNNNNNNTTGG 2 cut(s) 426, 458
AleI CACNNNNGTG 1 cut(s) 206
AluBI AGCT 3 cut(s) 353, 648, 923
AluI AGCT 3 cut(s) 353, 648, 923
Alw21I GWGCWC 1 cut(s) 516
Alw26I GTCTC 2 cut(s) 821, 1133
AlwI GGATC 2 cut(s) 947, 971
AoxI GGCC 3 cut(s) 61, 310, 1020
ApeKI GCWGC 2 cut(s) 467, 673
ApoI RAATTY 3 cut(s) 151, 934, 1186
ArsI GACNNNNNNTTYG 2 cut(s) 161, 193
AspLEI GCGC 1 cut(s) 383
AspS9I GGNCC 2 cut(s) 605, 1020
AsuHPI GGTGA 6 cut(s) 367, 414, 498, 742, 817, 1139
AsuII TTCGAA 1 cut(s) 708
AvaII GGWCC 1 cut(s) 605
AxyI CCTNAGG 1 cut(s) 1008
BalI TGGCCA 2 cut(s) 63, 312
Bbv12I GWGCWC 1 cut(s) 516
BbvI GCAGC 2 cut(s) 454, 660
BccI CCATC 8 cut(s) 212, 338, 434, 596, 605, 1007, 1011, 1034
BceAI ACGGC 2 cut(s) 415, 1156
BciT130I CCWGG 7 cut(s) 69, 267, 309, 447, 547, 802, 1050
BciVI GTATCC 1 cut(s) 1096
BclI TGATCA 1 cut(s) 118
BcoDI GTCTC 2 cut(s) 821, 1133
BfmI CTRYAG 3 cut(s) 237, 519, 685
BfuI GTATCC 1 cut(s) 1096
BisI GCNGC 3 cut(s) 468, 674, 731
BlpI GCTNAGC 1 cut(s) 1206
BlsI GCNGC 3 cut(s) 469, 675, 732
Bme1390I CCNGG 7 cut(s) 69, 267, 309, 447, 547, 802, 1050
Bme18I GGWCC 1 cut(s) 605
BmgT120I GGNCC 2 cut(s) 605, 1020
BmiI GGNNCC 1 cut(s) 799
BmrFI CCNGG 7 cut(s) 69, 267, 309, 447, 547, 802, 1050
BmrI ACTGGG 1 cut(s) 995
BmsI GCATC 2 cut(s) 512, 635
BmuI ACTGGG 1 cut(s) 995
BoxI GACNNNNGTC 1 cut(s) 857
Bpu1102I GCTNAGC 1 cut(s) 1206
Bpu14I TTCGAA 1 cut(s) 708
BsaAI YACGTR 1 cut(s) 913
BsaBI GATNNNNATC 2 cut(s) 195, 615
BsaJI CCNNGG 6 cut(s) 265, 793, 801, 847, 1048, 1049
BsaWI WCCGGW 1 cut(s) 508
BsaXI ACNNNNNCTCC 4 cut(s) 289, 319, 912, 942
Bsc4I CCNNNNNNNGG 2 cut(s) 1013, 1178
Bse1I ACTGG 2 cut(s) 703, 1001
Bse21I CCTNAGG 1 cut(s) 1008
Bse3DI GCAATG 1 cut(s) 639
Bse8I GATNNNNATC 2 cut(s) 195, 615
BseBI CCWGG 7 cut(s) 69, 267, 309, 447, 547, 802, 1050
BseDI CCNNGG 6 cut(s) 265, 793, 801, 847, 1048, 1049
BseGI GGATG 5 cut(s) 223, 555, 880, 1018, 1022
BseJI GATNNNNATC 2 cut(s) 195, 615
BseLI CCNNNNNNNGG 2 cut(s) 1013, 1178
BseMI GCAATG 1 cut(s) 639
BseMII CTCAG 3 cut(s) 768, 882, 1197
BseNI ACTGG 2 cut(s) 703, 1001
BseRI GAGGAG 1 cut(s) 1151
BseXI GCAGC 2 cut(s) 454, 660
BsgI GTGCAG 1 cut(s) 453
Bsh1285I CGRYCG 1 cut(s) 1081
BshFI GGCC 3 cut(s) 63, 312, 1022
BsiEI CGRYCG 1 cut(s) 1081
BsiHKAI GWGCWC 1 cut(s) 516
BsiSI CCGG 1 cut(s) 509
BslFI GGGAC 2 cut(s) 570, 865
BslI CCNNNNNNNGG 2 cut(s) 1013, 1178
BsmAI GTCTC 2 cut(s) 821, 1133
BsmBI CGTCTC 1 cut(s) 1133
BsmFI GGGAC 2 cut(s) 570, 865
BsnI GGCC 3 cut(s) 63, 312, 1022
Bsp119I TTCGAA 1 cut(s) 708
Bsp1286I GDGCHC 1 cut(s) 516
Bsp143I GATC 5 cut(s) 118, 190, 952, 976, 1078
Bsp1720I GCTNAGC 1 cut(s) 1206
BspACI CCGC 4 cut(s) 113, 731, 1107, 1120
BspANI GGCC 3 cut(s) 63, 312, 1022
BspCNI CTCAG 3 cut(s) 767, 881, 1198
BspHI TCATGA 1 cut(s) 861
BspLI GGNNCC 1 cut(s) 799
BspMAI CTGCAG 1 cut(s) 523
BspPI GGATC 2 cut(s) 947, 971
BspT104I TTCGAA 1 cut(s) 708
BsrDI GCAATG 1 cut(s) 639
BsrI ACTGG 2 cut(s) 703, 1001
BssECI CCNNGG 6 cut(s) 265, 793, 801, 847, 1048, 1049
BssMI GATC 5 cut(s) 118, 190, 952, 976, 1078
BssT1I CCWWGG 2 cut(s) 793, 847
Bst2UI CCWGG 7 cut(s) 69, 267, 309, 447, 547, 802, 1050
Bst4CI ACNGT 3 cut(s) 489, 832, 1199
Bst6I CTCTTC 1 cut(s) 488
BstBAI YACGTR 1 cut(s) 913
BstBI TTCGAA 1 cut(s) 708
BstC8I GCNNGC 5 cut(s) 65, 472, 575, 624, 678
BstDEI CTNAG 4 cut(s) 754, 868, 1008, 1206
BstF5I GGATG 5 cut(s) 223, 555, 880, 1018, 1022
BstHHI GCGC 1 cut(s) 383
BstKTI GATC 5 cut(s) 121, 193, 955, 979, 1081
BstMAI GTCTC 2 cut(s) 821, 1133
BstMBI GATC 5 cut(s) 118, 190, 952, 976, 1078
BstMCI CGRYCG 1 cut(s) 1081
BstNI CCWGG 7 cut(s) 69, 267, 309, 447, 547, 802, 1050
BstNSI RCATGY 5 cut(s) 175, 325, 577, 600, 1171
BstPAI GACNNNNGTC 1 cut(s) 857
BstSCI CCNGG 7 cut(s) 67, 265, 307, 445, 545, 800, 1048
BstSFI CTRYAG 3 cut(s) 237, 519, 685
BstV1I GCAGC 2 cut(s) 454, 660
BstXI CCANNNNNNTGG 3 cut(s) 308, 446, 1056
Bsu36I CCTNAGG 1 cut(s) 1008
BsuI GTATCC 1 cut(s) 1096
BsuRI GGCC 3 cut(s) 63, 312, 1022
BtsCI GGATG 5 cut(s) 223, 555, 880, 1018, 1022
BtsI GCAGTG 1 cut(s) 1066
BtsIMutI CAGTG 2 cut(s) 948, 1066
Cac8I GCNNGC 5 cut(s) 65, 472, 575, 624, 678
CciI TCATGA 1 cut(s) 861
CfoI GCGC 1 cut(s) 383
Cfr13I GGNCC 2 cut(s) 605, 1020
CseI GACGC 1 cut(s) 473
Csp6I GTAC 3 cut(s) 129, 158, 394
CspCI CAANNNNNGTGG 2 cut(s) 4, 39
CviQI GTAC 3 cut(s) 129, 158, 394
DdeI CTNAG 4 cut(s) 754, 868, 1008, 1206
DpnI GATC 5 cut(s) 120, 192, 954, 978, 1080
DpnII GATC 5 cut(s) 118, 190, 952, 976, 1078
DrdI GACNNNNNNGTC 2 cut(s) 287, 371
DseDI GACNNNNNNGTC 2 cut(s) 287, 371
EaeI YGGCCR 2 cut(s) 61, 310
Eam1104I CTCTTC 1 cut(s) 488
EarI CTCTTC 1 cut(s) 488
Eco130I CCWWGG 2 cut(s) 793, 847
Eco47I GGWCC 1 cut(s) 605
Eco81I CCTNAGG 1 cut(s) 1008
EcoRI GAATTC 1 cut(s) 151
EcoRII CCWGG 7 cut(s) 67, 265, 307, 445, 545, 800, 1048
EcoT14I CCWWGG 2 cut(s) 793, 847
ErhI CCWWGG 2 cut(s) 793, 847
Esp3I CGTCTC 1 cut(s) 1133
FaqI GGGAC 2 cut(s) 570, 865
FauNDI CATATG 1 cut(s) 1086
FbaI TGATCA 1 cut(s) 118
Fnu4HI GCNGC 3 cut(s) 468, 674, 731
FokI GGATG 5 cut(s) 230, 562, 867, 1025, 1029
Fsp4HI GCNGC 3 cut(s) 468, 674, 731
GlaI GCGC 1 cut(s) 382
GluI GCNGC 3 cut(s) 468, 674, 731
HaeIII GGCC 3 cut(s) 63, 312, 1022
HapII CCGG 1 cut(s) 509
HgaI GACGC 1 cut(s) 473
HhaI GCGC 1 cut(s) 383
Hin6I GCGC 1 cut(s) 381
HinP1I GCGC 1 cut(s) 381
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HinfI GANTC 3 cut(s) 44, 877, 1032
HpaII CCGG 1 cut(s) 509
HphI GGTGA 6 cut(s) 367, 414, 498, 742, 817, 1139
Hpy166II GTNNAC 1 cut(s) 463
Hpy188I TCNGA 3 cut(s) 43, 757, 1031
Hpy188III TCNNGA 2 cut(s) 862, 870
Hpy8I GTNNAC 1 cut(s) 463
HpyAV CCTTC 2 cut(s) 669, 1006
HpyCH4III ACNGT 3 cut(s) 489, 832, 1199
HpyCH4IV ACGT 7 cut(s) 228, 416, 459, 525, 777, 782, 912
HpyCH4V TGCA 8 cut(s) 470, 521, 626, 632, 676, 889, 895, 1066
HpyF3I CTNAG 4 cut(s) 754, 868, 1008, 1206
HpySE526I ACGT 7 cut(s) 228, 416, 459, 525, 777, 782, 912
HspAI GCGC 1 cut(s) 381
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 5 cut(s) 118, 190, 952, 976, 1078
LmnI GCTCC 2 cut(s) 511, 920
Lsp1109I GCAGC 2 cut(s) 454, 660
LweI GCATC 2 cut(s) 512, 635
MaeII ACGT 7 cut(s) 228, 416, 459, 525, 777, 782, 912
MaeIII GTNAC 8 cut(s) 221, 241, 289, 373, 778, 788, 946, 1127
MalI GATC 5 cut(s) 120, 192, 954, 978, 1080
MboI GATC 5 cut(s) 118, 190, 952, 976, 1078
MboII GAAGA 4 cut(s) 128, 169, 505, 820
MhlI GDGCHC 1 cut(s) 516
MlsI TGGCCA 2 cut(s) 63, 312
MluCI AATT 5 cut(s) 151, 817, 934, 967, 1186
MluNI TGGCCA 2 cut(s) 63, 312
MmeI TCCRAC 4 cut(s) 21, 114, 898, 1206
MnlI CCTC 6 cut(s) 315, 540, 763, 1129, 1164, 1222
Mox20I TGGCCA 2 cut(s) 63, 312
MscI TGGCCA 2 cut(s) 63, 312
MseI TTAA 4 cut(s) 29, 785, 959, 990
MslI CAYNNNNRTG 6 cut(s) 170, 206, 548, 572, 668, 894
Msp20I TGGCCA 2 cut(s) 63, 312
MspI CCGG 1 cut(s) 509
MspR9I CCNGG 7 cut(s) 69, 267, 309, 447, 547, 802, 1050
MvaI CCWGG 7 cut(s) 69, 267, 309, 447, 547, 802, 1050
NdeI CATATG 1 cut(s) 1086
NdeII GATC 5 cut(s) 118, 190, 952, 976, 1078
NlaIV GGNNCC 1 cut(s) 799
NmuCI GTSAC 5 cut(s) 289, 373, 788, 946, 1127
NspI RCATGY 5 cut(s) 175, 325, 577, 600, 1171
NspV TTCGAA 1 cut(s) 708
OliI CACNNNNGTG 1 cut(s) 206
PaeI GCATGC 1 cut(s) 577
PagI TCATGA 1 cut(s) 861
PasI CCCWGGG 1 cut(s) 1049
PciI ACATGT 2 cut(s) 171, 596
PfeI GAWTC 3 cut(s) 44, 877, 1032
PflFI GACNNNGTC 1 cut(s) 944
PkrI GCNGC 3 cut(s) 469, 675, 732
Ple19I CGATCG 1 cut(s) 1081
Ppu21I YACGTR 1 cut(s) 913
PscI ACATGT 2 cut(s) 171, 596
PshAI GACNNNNGTC 1 cut(s) 857
PsiI TTATAA 1 cut(s) 329
Psp1406I AACGTT 1 cut(s) 777
Psp6I CCWGG 7 cut(s) 67, 265, 307, 445, 545, 800, 1048
PspGI CCWGG 7 cut(s) 67, 265, 307, 445, 545, 800, 1048
PspN4I GGNNCC 1 cut(s) 799
PspPI GGNCC 2 cut(s) 605, 1020
PstI CTGCAG 1 cut(s) 523
PsyI GACNNNGTC 1 cut(s) 944
PvuI CGATCG 1 cut(s) 1081
RsaI GTAC 3 cut(s) 130, 159, 395
RsaNI GTAC 3 cut(s) 129, 158, 394
RseI CAYNNNNRTG 6 cut(s) 170, 206, 548, 572, 668, 894
SaqAI TTAA 4 cut(s) 29, 785, 959, 990
SatI GCNGC 3 cut(s) 468, 674, 731
Sau3AI GATC 5 cut(s) 118, 190, 952, 976, 1078
Sau96I GGNCC 2 cut(s) 605, 1020
ScrFI CCNGG 7 cut(s) 69, 267, 309, 447, 547, 802, 1050
SduI GDGCHC 1 cut(s) 516
SfaNI GCATC 2 cut(s) 512, 635
SfcI CTRYAG 3 cut(s) 237, 519, 685
SfuI TTCGAA 1 cut(s) 708
SinI GGWCC 1 cut(s) 605
SmiMI CAYNNNNRTG 6 cut(s) 170, 206, 548, 572, 668, 894
SphI GCATGC 1 cut(s) 577
Sse9I AATT 5 cut(s) 151, 817, 934, 967, 1186
SsiI CCGC 4 cut(s) 113, 731, 1107, 1120
SspI AATATT 1 cut(s) 387
StyD4I CCNGG 7 cut(s) 67, 265, 307, 445, 545, 800, 1048
StyI CCWWGG 2 cut(s) 793, 847
TaaI ACNGT 3 cut(s) 489, 832, 1199
TaiI ACGT 7 cut(s) 231, 419, 462, 528, 780, 785, 915
TaqI TCGA 5 cut(s) 106, 342, 363, 708, 939
TasI AATT 5 cut(s) 151, 817, 934, 967, 1186
TatI WGTACW 1 cut(s) 393
TauI GCSGC 1 cut(s) 733
TfiI GAWTC 3 cut(s) 44, 877, 1032
Tru1I TTAA 4 cut(s) 29, 785, 959, 990
Tru9I TTAA 4 cut(s) 29, 785, 959, 990
TscAI CASTG 2 cut(s) 955, 1073
TseFI GTSAC 5 cut(s) 289, 373, 788, 946, 1127
TseI GCWGC 2 cut(s) 467, 673
Tsp45I GTSAC 5 cut(s) 289, 373, 788, 946, 1127
TspDTI ATGAA 4 cut(s) 650, 878, 1051, 1102
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 2 cut(s) 955, 1073
Tth111I GACNNNGTC 1 cut(s) 944
VpaK11BI GGWCC 1 cut(s) 605
XapI RAATTY 3 cut(s) 151, 934, 1186
XceI RCATGY 5 cut(s) 175, 325, 577, 600, 1171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.