Rorug01G0421100

At4g00950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007943
Physical Location & Seq
Forward (+)
52240024 .. 52242317
2294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0421100.1

Sequence Viewer

Length: 855 bp
ATGGAAGGGCATTGTGAAAGGAAAACATGGCTAAACAATTCGTCATACATTGCTAATGAAACGGAGAACTCGAATTTACGCTTTTATGCTAGGGTTAAAGGAAGTTTTGTGCTTAGATTTGGTTGTTATGAGAGGCCCAACTTGACGGCTTGTAACCCGAACCCACCACTAAAACCCTTACTTAAAAACCCTACAAGCTCAGAGCTCACGGCGGGCAAGAGACTCAGAGACACTGCGACAATGGGGGCTTACAAGTACGTATCGGAGATATGGAGGAAGAAGCAGTCGGACGTTATGAACTTCGTTTCCAGAATCCGAGTTTGGCAGTACCGCCAGGAACCCTCCATTGTCCGCGTCACTCGTCCCACTCGCCCTGACAAGGCTCGTCGCTTGGGCTACAAGGCCAAGCAGGGGTACGTTATCTACCGGGTGCGTGTGAGGCGTGGTGGACGCAAGAGACCAGTTGCGAAGGGTATTGTGTATGGCAAGCCCACAAACCAGGGTGTTACTCAATTGAAGTTCCAACGCAGCAAGCGCTCAGTTGCAGAGGAGCGTGCAGGTCGCAAACTAGGTGGCCTTAAGGTTCTCAATTCTTACTGGGTTAATGAGGATTCTACATACAAATATTTTGAGGTGATCTTGGTTGATGCTGCCCATATGACCATCCAAAATGACCGAAGGATTAATTGGATCTGCAATCCTGTTCACAAGCACAGAGAGCTTCGTGGACTCACTTCTGCTGGAAAGAAATATAGGGGTCTGCAAGGAAAGGGTCACTTGCACCACAAGGCACGACCTTCAAGGAGGGCCAACTGGAAGAGGAACAACACCCTTTCTCTTCGGCGTTACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF688

Protein Analysis

284

Amino Acids

33.22

Weight (kDa)

10.95

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L15e PF00827 82 - 271 8.2e-90 Ribosomal L15
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 548
AccII CGCG 1 cut(s) 354
AciI CCGC 3 cut(s) 212, 331, 352
AclWI GGATC 1 cut(s) 698
AcsI RAATTY 1 cut(s) 73
AfaI GTAC 3 cut(s) 257, 329, 416
AfeI AGCGCT 1 cut(s) 536
AfiI CCNNNNNNNGG 2 cut(s) 379, 411
AflII CTTAAG 1 cut(s) 578
AgsI TTSAA 2 cut(s) 517, 801
AjnI CCWGG 2 cut(s) 333, 498
AjuI GAANNNNNNNTTGG 4 cut(s) 304, 336, 670, 702
AloI GAACNNNNNNTCC 2 cut(s) 290, 322
AluBI AGCT 3 cut(s) 198, 205, 721
AluI AGCT 3 cut(s) 198, 205, 721
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 3 cut(s) 214, 222, 451
AlwI GGATC 1 cut(s) 698
Aor51HI AGCGCT 1 cut(s) 536
AoxI GGCC 4 cut(s) 134, 402, 574, 807
ApeKI GCWGC 2 cut(s) 528, 650
ApoI RAATTY 1 cut(s) 73
AseI ATTAAT 1 cut(s) 684
AspLEI GCGC 1 cut(s) 537
AspS9I GGNCC 2 cut(s) 135, 807
AsuC2I CCSGG 1 cut(s) 428
AsuHPI GGTGA 1 cut(s) 646
BanII GRGCYC 1 cut(s) 207
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 2 cut(s) 540, 637
BccI CCATC 1 cut(s) 671
BceAI ACGGC 2 cut(s) 162, 225
BciT130I CCWGG 2 cut(s) 335, 500
BcnI CCSGG 1 cut(s) 428
BcoDI GTCTC 3 cut(s) 214, 222, 451
BfaI CTAG 2 cut(s) 90, 569
BfoI RGCGCY 1 cut(s) 538
BfrI CTTAAG 1 cut(s) 578
BfuAI ACCTGC 1 cut(s) 548
BisI GCNGC 2 cut(s) 529, 651
BlsI GCNGC 2 cut(s) 530, 652
Bme1390I CCNGG 3 cut(s) 335, 428, 500
BmgT120I GGNCC 2 cut(s) 135, 807
BmiI GGNNCC 1 cut(s) 339
BmrFI CCNGG 3 cut(s) 335, 428, 500
BmrI ACTGGG 1 cut(s) 607
BmsI GCATC 1 cut(s) 637
BmuI ACTGGG 1 cut(s) 607
BpuMI CCSGG 1 cut(s) 428
BsaAI YACGTR 1 cut(s) 259
BsaI GGTCTC 1 cut(s) 451
BsaJI CCNNGG 1 cut(s) 499
Bsc4I CCNNNNNNNGG 2 cut(s) 379, 411
Bse1I ACTGG 3 cut(s) 461, 602, 818
Bse3DI GCAATG 1 cut(s) 48
BseBI CCWGG 2 cut(s) 335, 500
BseDI CCNNGG 1 cut(s) 499
BseGI GGATG 1 cut(s) 663
BseLI CCNNNNNNNGG 2 cut(s) 379, 411
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 3 cut(s) 213, 238, 552
BseNI ACTGG 3 cut(s) 461, 602, 818
BseRI GAGGAG 1 cut(s) 563
BseXI GCAGC 2 cut(s) 540, 637
BsgI GTGCAG 1 cut(s) 576
Bsh1236I CGCG 1 cut(s) 354
BshFI GGCC 4 cut(s) 136, 404, 576, 809
BsiHKAI GWGCWC 1 cut(s) 207
BsiSI CCGG 1 cut(s) 427
BslFI GGGAC 1 cut(s) 348
BslI CCNNNNNNNGG 2 cut(s) 379, 411
BsmAI GTCTC 3 cut(s) 214, 222, 451
BsmFI GGGAC 1 cut(s) 348
BsnI GGCC 4 cut(s) 136, 404, 576, 809
Bso31I GGTCTC 1 cut(s) 451
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 2 cut(s) 636, 690
BspACI CCGC 3 cut(s) 212, 331, 352
BspANI GGCC 4 cut(s) 136, 404, 576, 809
BspCNI CTCAG 3 cut(s) 212, 237, 551
BspFNI CGCG 1 cut(s) 354
BspLI GGNNCC 1 cut(s) 339
BspMI ACCTGC 1 cut(s) 548
BspPI GGATC 1 cut(s) 698
BspTI CTTAAG 1 cut(s) 578
BspTNI GGTCTC 1 cut(s) 451
BsrDI GCAATG 1 cut(s) 48
BsrI ACTGG 3 cut(s) 461, 602, 818
BssECI CCNNGG 1 cut(s) 499
BssMI GATC 2 cut(s) 636, 690
Bst2UI CCWGG 2 cut(s) 335, 500
Bst4CI ACNGT 1 cut(s) 851
Bst6I CTCTTC 2 cut(s) 812, 843
BstAFI CTTAAG 1 cut(s) 578
BstBAI YACGTR 1 cut(s) 259
BstC8I GCNNGC 4 cut(s) 214, 488, 533, 555
BstDEI CTNAG 4 cut(s) 113, 199, 224, 538
BstF5I GGATG 1 cut(s) 663
BstFNI CGCG 1 cut(s) 354
BstH2I RGCGCY 1 cut(s) 538
BstHHI GCGC 1 cut(s) 537
BstKTI GATC 2 cut(s) 639, 693
BstMAI GTCTC 3 cut(s) 214, 222, 451
BstMBI GATC 2 cut(s) 636, 690
BstMWI GCNNNNNNNGC 3 cut(s) 439, 534, 718
BstNI CCWGG 2 cut(s) 335, 500
BstSCI CCNGG 3 cut(s) 333, 426, 498
BstSNI TACGTA 1 cut(s) 259
BstUI CGCG 1 cut(s) 354
BstV1I GCAGC 2 cut(s) 540, 637
BstX2I RGATCY 1 cut(s) 690
BstYI RGATCY 1 cut(s) 690
BsuRI GGCC 4 cut(s) 136, 404, 576, 809
BtsCI GGATG 1 cut(s) 663
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 1 cut(s) 231
BveI ACCTGC 1 cut(s) 548
Cac8I GCNNGC 4 cut(s) 214, 488, 533, 555
CfoI GCGC 1 cut(s) 537
Cfr13I GGNCC 2 cut(s) 135, 807
CseI GACGC 2 cut(s) 343, 459
Csp6I GTAC 3 cut(s) 256, 328, 415
CspCI CAANNNNNGTGG 2 cut(s) 553, 588
CviAII CATG 1 cut(s) 27
CviQI GTAC 3 cut(s) 256, 328, 415
DdeI CTNAG 4 cut(s) 113, 199, 224, 538
DpnI GATC 2 cut(s) 638, 692
DpnII GATC 2 cut(s) 636, 690
Eam1104I CTCTTC 2 cut(s) 812, 843
EarI CTCTTC 2 cut(s) 812, 843
Ecl136II GAGCTC 1 cut(s) 205
Eco105I TACGTA 1 cut(s) 259
Eco24I GRGCYC 1 cut(s) 207
Eco31I GGTCTC 1 cut(s) 451
Eco47III AGCGCT 1 cut(s) 536
Eco53kI GAGCTC 1 cut(s) 205
EcoICRI GAGCTC 1 cut(s) 205
EcoRII CCWGG 2 cut(s) 333, 498
EcoT38I GRGCYC 1 cut(s) 207
FaeI CATG 1 cut(s) 30
FalI AAGNNNNNCTT 2 cut(s) 761, 793
FaqI GGGAC 1 cut(s) 348
FatI CATG 1 cut(s) 26
FauI CCCGC 1 cut(s) 205
FauNDI CATATG 1 cut(s) 657
Fnu4HI GCNGC 2 cut(s) 529, 651
FokI GGATG 1 cut(s) 650
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 2 cut(s) 529, 651
FspBI CTAG 2 cut(s) 90, 569
GlaI GCGC 1 cut(s) 536
GluI GCNGC 2 cut(s) 529, 651
HaeII RGCGCY 1 cut(s) 538
HaeIII GGCC 4 cut(s) 136, 404, 576, 809
HapII CCGG 1 cut(s) 427
HgaI GACGC 2 cut(s) 343, 459
HhaI GCGC 1 cut(s) 537
Hin1II CATG 1 cut(s) 30
Hin6I GCGC 1 cut(s) 535
HinP1I GCGC 1 cut(s) 535
HinfI GANTC 4 cut(s) 222, 312, 611, 729
HpaII CCGG 1 cut(s) 427
HphI GGTGA 1 cut(s) 646
Hpy166II GTNNAC 3 cut(s) 449, 706, 728
Hpy188I TCNGA 5 cut(s) 202, 227, 265, 289, 317
Hpy188III TCNNGA 1 cut(s) 309
Hpy8I GTNNAC 3 cut(s) 449, 706, 728
Hpy99I CGWCG 1 cut(s) 390
HpyAV CCTTC 3 cut(s) 463, 672, 807
HpyCH4III ACNGT 1 cut(s) 851
HpyCH4IV ACGT 3 cut(s) 258, 291, 417
HpyCH4V TGCA 5 cut(s) 545, 557, 696, 763, 781
HpyF10VI GCNNNNNNNGC 3 cut(s) 439, 534, 718
HpyF3I CTNAG 4 cut(s) 113, 199, 224, 538
HpySE526I ACGT 3 cut(s) 258, 291, 417
Hsp92II CATG 1 cut(s) 30
HspAI GCGC 1 cut(s) 535
Kzo9I GATC 2 cut(s) 636, 690
LmnI GCTCC 1 cut(s) 550
Lsp1109I GCAGC 2 cut(s) 540, 637
LweI GCATC 1 cut(s) 637
MaeI CTAG 2 cut(s) 90, 569
MaeII ACGT 3 cut(s) 258, 291, 417
MaeIII GTNAC 5 cut(s) 152, 355, 505, 773, 845
MalI GATC 2 cut(s) 638, 692
MboI GATC 2 cut(s) 636, 690
MboII GAAGA 3 cut(s) 289, 829, 830
MfeI CAATTG 1 cut(s) 512
MflI RGATCY 1 cut(s) 690
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 5 cut(s) 37, 73, 512, 589, 685
MlyI GAGTC 2 cut(s) 216, 723
MmeI TCCRAC 2 cut(s) 267, 547
MnlI CCTC 9 cut(s) 126, 267, 352, 432, 541, 601, 625, 798, 813
MseI TTAA 5 cut(s) 96, 183, 579, 603, 684
MspCI CTTAAG 1 cut(s) 578
MspI CCGG 1 cut(s) 427
MspR9I CCNGG 3 cut(s) 335, 428, 500
MunI CAATTG 1 cut(s) 512
MvaI CCWGG 2 cut(s) 335, 500
MvnI CGCG 1 cut(s) 354
MwoI GCNNNNNNNGC 3 cut(s) 439, 534, 718
NciI CCSGG 1 cut(s) 428
NdeI CATATG 1 cut(s) 657
NdeII GATC 2 cut(s) 636, 690
NlaIII CATG 1 cut(s) 30
NlaIV GGNNCC 1 cut(s) 339
NmuCI GTSAC 2 cut(s) 355, 773
PcsI WCGNNNNNNNCGW 2 cut(s) 68, 847
PfeI GAWTC 2 cut(s) 312, 611
PkrI GCNGC 2 cut(s) 530, 652
PleI GAGTC 2 cut(s) 216, 723
PpsI GAGTC 2 cut(s) 216, 723
Ppu21I YACGTR 1 cut(s) 259
PshBI ATTAAT 1 cut(s) 684
Psp124BI GAGCTC 1 cut(s) 207
Psp6I CCWGG 2 cut(s) 333, 498
PspGI CCWGG 2 cut(s) 333, 498
PspN4I GGNNCC 1 cut(s) 339
PspPI GGNCC 2 cut(s) 135, 807
PsuI RGATCY 1 cut(s) 690
RsaI GTAC 3 cut(s) 257, 329, 416
RsaNI GTAC 3 cut(s) 256, 328, 415
SacI GAGCTC 1 cut(s) 207
SaqAI TTAA 5 cut(s) 96, 183, 579, 603, 684
SatI GCNGC 2 cut(s) 529, 651
Sau3AI GATC 2 cut(s) 636, 690
Sau96I GGNCC 2 cut(s) 135, 807
SchI GAGTC 2 cut(s) 216, 723
ScrFI CCNGG 3 cut(s) 335, 428, 500
SduI GDGCHC 1 cut(s) 207
SfaNI GCATC 1 cut(s) 637
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
SnaBI TACGTA 1 cut(s) 259
Sse9I AATT 5 cut(s) 37, 73, 512, 589, 685
SsiI CCGC 3 cut(s) 212, 331, 352
SspI AATATT 1 cut(s) 626
SspMI CTAG 2 cut(s) 90, 569
SstI GAGCTC 1 cut(s) 207
StyD4I CCNGG 3 cut(s) 333, 426, 498
TaaI ACNGT 1 cut(s) 851
TaiI ACGT 3 cut(s) 261, 294, 420
TaqI TCGA 1 cut(s) 71
TaqII GACCGA 1 cut(s) 690
TasI AATT 5 cut(s) 37, 73, 512, 589, 685
TfiI GAWTC 2 cut(s) 312, 611
Tru1I TTAA 5 cut(s) 96, 183, 579, 603, 684
Tru9I TTAA 5 cut(s) 96, 183, 579, 603, 684
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 2 cut(s) 355, 773
TseI GCWGC 2 cut(s) 528, 650
Tsp45I GTSAC 2 cut(s) 355, 773
TspDTI ATGAA 2 cut(s) 72, 311
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 1 cut(s) 238
Vha464I CTTAAG 1 cut(s) 578
VspI ATTAAT 1 cut(s) 684
XapI RAATTY 1 cut(s) 73
XspI CTAG 2 cut(s) 90, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.