Rorug02G0036900

Chloroplastic group IIA intron splicing facilitator CRS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010924
Physical Location & Seq
Reverse (-)
2901127 .. 2905102
3976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0036900.1

Sequence Viewer

Length: 435 bp
ATGCGTAACTTTACCATTCATCCAGGAAATAGCTTCTCAAAATTTCATCCCAGCTCTTCTAACTTCTATCATTCCATTTATTCATCTACTACCACTACACGTACTAATACTCTTGCAATGGCAAAGAATGTGGCTCAGTCTCCCATGGAGAAACGCAGCCTGACTTCATTTGACCAGCAGTTGGCTAAGCGCTGTTCTCGTGAGGGTGTAAAAGCTGGAGCTAAGGCAGCTGTTGTTGCTAGCATTGCCACTGCAATTCCAACTTTTGCTAGTGTGAGGATGCTGCCATGGGCAAAAGCCAATCTGAATCCCACTGCTCAGGCTCTCATAATCTCCACAGCGGCTGGAATGGCATATTTCATAGTGGCTGACAAGACTATTTTGGCAACCGCAAGGAAGAACTCATTCCACCAAGGATCTAACTATGAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

144

Amino Acids

15.56

Weight (kDa)

10.34

Isoelectric Point (pI)

32.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENOD93 PF03386 61 - 135 1.2e-39 Early nodulin 93 ENOD93 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010924)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29750 AT4G29750
fragaria_vesca FvH4_1g07640 FvH4_1g07640 FvH4_1g07640 FvH4_1g07640
malus_domestica MD15G1208500.v1.1
prunus_persica Prupe.7G208300_v2.0.a1 Prupe.7G208300_v2.0.a1
pyrus_communis pycom15g18460
rosa_chinensis RchiOBHm_Chr2g0093501
rosa_laevigata RLG00000016412
rosa_multiflora Rmu_sc0004423.1_g000007
rosa_roxburghii Rroxscaffold_2G00148030
rosa_rugosa Rorug02G0036900
rosa_samantha Rh2AG083300 Rh2BG084000 Rh2CG086200 Rh2DG082100
rosa_wichuraiana Rw2G006410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 181
AciI CCGC 2 cut(s) 341, 390
AclWI GGATC 1 cut(s) 424
AcsI RAATTY 1 cut(s) 41
AfaI GTAC 1 cut(s) 103
AfeI AGCGCT 1 cut(s) 191
AfiI CCNNNNNNNGG 1 cut(s) 181
AflIII ACRYGT 1 cut(s) 98
AjnI CCWGG 1 cut(s) 22
AluBI AGCT 5 cut(s) 33, 54, 215, 221, 230
AluI AGCT 5 cut(s) 33, 54, 215, 221, 230
Alw26I GTCTC 1 cut(s) 144
AlwI GGATC 1 cut(s) 424
AlwNI CAGNNNCTG 1 cut(s) 344
Aor51HI AGCGCT 1 cut(s) 191
ApeKI GCWGC 3 cut(s) 156, 227, 283
ApoI RAATTY 1 cut(s) 41
Asp700I GAANNNNTTC 1 cut(s) 404
AspLEI GCGC 1 cut(s) 192
AsuNHI GCTAGC 1 cut(s) 239
BauI CACGAG 1 cut(s) 198
BbvI GCAGC 3 cut(s) 168, 239, 270
BciT130I CCWGG 1 cut(s) 24
BcoDI GTCTC 1 cut(s) 144
BfaI CTAG 2 cut(s) 240, 270
BfoI RGCGCY 1 cut(s) 193
BisI GCNGC 4 cut(s) 157, 228, 284, 342
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 4 cut(s) 158, 229, 285, 343
Bme1390I CCNGG 1 cut(s) 24
BmrFI CCNGG 1 cut(s) 24
BmsI GCATC 1 cut(s) 270
BmtI GCTAGC 1 cut(s) 243
BpmI CTGGAG 1 cut(s) 237
Bpu10I CCTNAGC 2 cut(s) 222, 318
Bpu1102I GCTNAGC 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 101
BsaJI CCNNGG 3 cut(s) 144, 287, 412
Bsc4I CCNNNNNNNGG 1 cut(s) 181
Bse3DI GCAATG 2 cut(s) 123, 243
BseBI CCWGG 1 cut(s) 24
BseDI CCNNGG 3 cut(s) 144, 287, 412
BseGI GGATG 3 cut(s) 19, 46, 285
BseLI CCNNNNNNNGG 1 cut(s) 181
BseMI GCAATG 2 cut(s) 123, 243
BseMII CTCAG 2 cut(s) 149, 332
BseXI GCAGC 3 cut(s) 168, 239, 270
BseYI CCCAGC 1 cut(s) 50
BslI CCNNNNNNNGG 1 cut(s) 181
BsmAI GTCTC 1 cut(s) 144
Bsp143I GATC 1 cut(s) 416
Bsp1720I GCTNAGC 1 cut(s) 186
Bsp19I CCATGG 2 cut(s) 144, 287
BspACI CCGC 2 cut(s) 341, 390
BspCNI CTCAG 2 cut(s) 148, 331
BspOI GCTAGC 1 cut(s) 243
BspPI GGATC 1 cut(s) 424
BspQI GCTCTTC 1 cut(s) 61
BsrDI GCAATG 2 cut(s) 123, 243
BssECI CCNNGG 3 cut(s) 144, 287, 412
BssMI GATC 1 cut(s) 416
BssSI CACGAG 1 cut(s) 198
BssT1I CCWWGG 3 cut(s) 144, 287, 412
Bst2BI CACGAG 1 cut(s) 198
Bst2UI CCWGG 1 cut(s) 24
Bst6I CTCTTC 1 cut(s) 61
BstBAI YACGTR 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 4 cut(s) 135, 186, 222, 318
BstDSI CCRYGG 2 cut(s) 144, 287
BstF5I GGATG 3 cut(s) 19, 46, 285
BstH2I RGCGCY 1 cut(s) 193
BstHHI GCGC 1 cut(s) 192
BstKTI GATC 1 cut(s) 419
BstMAI GTCTC 1 cut(s) 144
BstMBI GATC 1 cut(s) 416
BstMWI GCNNNNNNNGC 4 cut(s) 227, 236, 245, 350
BstNI CCWGG 1 cut(s) 24
BstSCI CCNGG 1 cut(s) 22
BstV1I GCAGC 3 cut(s) 168, 239, 270
BstX2I RGATCY 1 cut(s) 416
BstYI RGATCY 1 cut(s) 416
BtgI CCRYGG 2 cut(s) 144, 287
BtsCI GGATG 3 cut(s) 19, 46, 285
BtsI GCAGTG 2 cut(s) 249, 312
BtsIMutI CAGTG 2 cut(s) 249, 312
Cac8I GCNNGC 1 cut(s) 241
CaiI CAGNNNCTG 1 cut(s) 344
CfoI GCGC 1 cut(s) 192
Csp6I GTAC 1 cut(s) 102
CspCI CAANNNNNGTGG 2 cut(s) 111, 146
CviAII CATG 2 cut(s) 145, 288
CviQI GTAC 1 cut(s) 102
DdeI CTNAG 4 cut(s) 135, 186, 222, 318
DpnI GATC 1 cut(s) 418
DpnII GATC 1 cut(s) 416
Eam1104I CTCTTC 1 cut(s) 61
EarI CTCTTC 1 cut(s) 61
Eco130I CCWWGG 3 cut(s) 144, 287, 412
Eco47III AGCGCT 1 cut(s) 191
EcoRII CCWGG 1 cut(s) 22
EcoT14I CCWWGG 3 cut(s) 144, 287, 412
ErhI CCWWGG 3 cut(s) 144, 287, 412
FaeI CATG 2 cut(s) 148, 291
FaiI YATR 7 cut(s) 146, 289, 329, 355, 362, 426, 433
FatI CATG 2 cut(s) 144, 287
Fnu4HI GCNGC 4 cut(s) 157, 228, 284, 342
FokI GGATG 3 cut(s) 6, 33, 292
Fsp4HI GCNGC 4 cut(s) 157, 228, 284, 342
FspBI CTAG 2 cut(s) 240, 270
GlaI GCGC 1 cut(s) 191
GluI GCNGC 4 cut(s) 157, 228, 284, 342
GsaI CCCAGC 1 cut(s) 54
GsuI CTGGAG 1 cut(s) 237
HaeII RGCGCY 1 cut(s) 193
HhaI GCGC 1 cut(s) 192
Hin1II CATG 2 cut(s) 148, 291
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HinfI GANTC 1 cut(s) 307
Hpy188I TCNGA 1 cut(s) 306
Hpy188III TCNNGA 1 cut(s) 200
HpyCH4IV ACGT 1 cut(s) 100
HpyCH4V TGCA 2 cut(s) 116, 254
HpyF10VI GCNNNNNNNGC 4 cut(s) 227, 236, 245, 350
HpyF3I CTNAG 4 cut(s) 135, 186, 222, 318
HpySE526I ACGT 1 cut(s) 100
Hsp92II CATG 2 cut(s) 148, 291
HspAI GCGC 1 cut(s) 190
Kzo9I GATC 1 cut(s) 416
LguI GCTCTTC 1 cut(s) 61
LmnI GCTCC 1 cut(s) 218
LpnPI CCDG 8 cut(s) 9, 36, 64, 173, 188, 201, 305, 330
Lsp1109I GCAGC 3 cut(s) 168, 239, 270
LweI GCATC 1 cut(s) 270
MaeI CTAG 2 cut(s) 240, 270
MaeII ACGT 1 cut(s) 100
MaeIII GTNAC 1 cut(s) 5
MalI GATC 1 cut(s) 418
MboI GATC 1 cut(s) 416
MboII GAAGA 2 cut(s) 48, 409
MflI RGATCY 1 cut(s) 416
MluCI AATT 2 cut(s) 41, 255
MmeI TCCRAC 1 cut(s) 284
MnlI CCTC 2 cut(s) 196, 270
MroXI GAANNNNTTC 1 cut(s) 404
MspA1I CMGCKG 2 cut(s) 230, 341
MspR9I CCNGG 1 cut(s) 24
MvaI CCWGG 1 cut(s) 24
MwoI GCNNNNNNNGC 4 cut(s) 227, 236, 245, 350
NcoI CCATGG 2 cut(s) 144, 287
NdeII GATC 1 cut(s) 416
NheI GCTAGC 1 cut(s) 239
NlaIII CATG 2 cut(s) 148, 291
PciSI GCTCTTC 1 cut(s) 61
PdmI GAANNNNTTC 1 cut(s) 404
PfeI GAWTC 1 cut(s) 307
PflMI CCANNNNNTGG 1 cut(s) 181
PfoI TCCNGGA 1 cut(s) 22
PkrI GCNGC 4 cut(s) 158, 229, 285, 343
Ppu21I YACGTR 1 cut(s) 101
Psp6I CCWGG 1 cut(s) 22
PspFI CCCAGC 1 cut(s) 50
PspGI CCWGG 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 344
PsuI RGATCY 1 cut(s) 416
PvuII CAGCTG 1 cut(s) 230
RsaI GTAC 1 cut(s) 103
RsaNI GTAC 1 cut(s) 102
SapI GCTCTTC 1 cut(s) 61
SatI GCNGC 4 cut(s) 157, 228, 284, 342
Sau3AI GATC 1 cut(s) 416
ScrFI CCNGG 1 cut(s) 24
SetI ASST 6 cut(s) 35, 56, 103, 217, 223, 232
SfaNI GCATC 1 cut(s) 270
Sse9I AATT 2 cut(s) 41, 255
SsiI CCGC 2 cut(s) 341, 390
SspMI CTAG 2 cut(s) 240, 270
StyD4I CCNGG 1 cut(s) 22
StyI CCWWGG 3 cut(s) 144, 287, 412
TaiI ACGT 1 cut(s) 103
TasI AATT 2 cut(s) 41, 255
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 1 cut(s) 307
TscAI CASTG 2 cut(s) 256, 319
TseI GCWGC 3 cut(s) 156, 227, 283
TspDTI ATGAA 5 cut(s) 8, 35, 72, 156, 349
TspRI CASTG 2 cut(s) 256, 319
Van91I CCANNNNNTGG 1 cut(s) 181
XapI RAATTY 1 cut(s) 41
XmnI GAANNNNTTC 1 cut(s) 404
XspI CTAG 2 cut(s) 240, 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.