Rorug02G0060400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0017964
Physical Location & Seq
Forward (+)
4674800 .. 4679623
4824 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0060400.1

Sequence Viewer

Length: 2964 bp
ATGGCCCAGATTATCAGAGACAGAGTTTTCGGCTCCTTTGGTGGTTCCAGACGACACAGCAGAGTCCCTATTCCAATTCCAATTCCAGAACCCGAACCCGAACGCGTCGTCATGCCCGTTTACCCGGTCGAAGACCTGCCCAACCCGTTTGGGGACTTGGGCCCGCATCTCTCCGACTCGGACCTCCGAGAAACCGTCTACGAGATCCTAGTCGGAGCCTGCCGGAGCTCCGGCCCGAAACCGCTCACCTACACCCCGCAGTCGGAGAAGATCACCGTAGACCGGAATTCCCAGTCTTCTTCTTCTTCTTCTTCCTCGCTGTCGTCACTTCAGAGGTCGCTGACGTCATCGGCGAAGAAGGCTCTGGGTTTGAAGCAAACGGCGTCGTCTAAGAGGCTCGGTGGGAGTAGTAAACGGTCCGGGTCGGTTTTTGAGCTCATGCGGGTCCAGATGAGGGTTTCGGAGCAGACCGATACCCGAATCAGGAGAGCCCTTCTCAGAGTTGCTGCTGGTCAGCTCGGACGAAAGATAGAGAGCATGGTTTTGCCTCTGGAGCTGTTACAGCATTTCAGGTCGTTAGATTTTGCGAATCAGCAGGAGTATGAAGCTTGGCAGAGGAGGAATTTGAAGGTTCTTGAAGTGGGACTTATGGTGTATCCTCATATGCCGTTGGATAGAAAAGACACCGCTCCTCAACAGCTCCGGAAGATTATACGTGGGGCCTTGGAGAAACCCATGGAAACTGGAAAGAACACCGAGACAATGCAAGTTCTCCGCAGTCTTGTTATGTCTCTTGCCTGCAGATCGTTTGATGGTACTGTTTCCGACACATGCCACTGGGCAGATGGGTTTCCGTTGAATCTCAGGCTCTACCAAAAGCTCCTGGAATCATGTTTTGACCTGAATGAAGAAACTTCTATTATTGAAGAGCTTGATGAGGTTTTAGAAGTCATGAAGAAGACCTGGGTAGTCCTTGGAATAAACCAAATTCTGCATAATCTGTGTTTTGCATGGGTTTTATTTCATCGATATGTTACAACTGGCCAAGTGGATAATGACCTGCTGTTTGCAGCCAATAACCTCTTGGCGGAAGTTCAGCAAGATGCCAATGCCACAAAAGATCCATCTTATTTGAAGATCGTAAGTTCAACATTGAATACAATACTGGGTTGGACTGAGAGAAGGCTTCTTGCCTACCGTGATGTTTTCAACACTGGCAATATTGAGTCGATGCAAAACATTGTCTCCCTGGGGGTATTATCAGCAAAAGTACTAGTAGAGGATATCTCTCACGAGTATCGTAGGAAGAGAAAAGAAGTTGATGTGGCACGTGACAGGGTTGACACCTACATAAGATCATCAATACGCACCGCTTTTGCTCAGAAACTGGAAAAAGTGGGCTCCAACAAGCGCCTATCAAAGAGCCAAAAGAATCGTATTCCTACCTTGTCCGTCCTTGCAACAGAAGTTAGCGAACTGGCCTTTAGTGAGAAAGATATATTTGGTCCAGTGTTGAAGAGATGGCACCCTTTTGCAACGGGTGTTGCTATGGCTACGCTGCATTCTTGCTATGGAGACGAGCTCAAGCAATTTGTGGATGGTATCAGTGAGTTGACTCCTGATACTGTACAAGTACTAAAAGCTGCTGACAAATTGGAGAAAGATCTTGTGCAGATTGCTGTCGAAGATTCAGTAGATAGTGAGGATGGCGGGAAATCCATCATACAGGAGATGCCTCCTTTCGAGGCTGAAGCTGTAATGGCTGAGCTCGCGAAGGCTTGGATAAGGACAAGATTGGACAGGCTGAAGGAATGGGTTGACAGAAATCTGCAACTAGAGGTCTGGAATCCACAAGCGAACAAAGAACGCTTTGCTCCTTCTGCCATTGAAGTTTTACGCATTGTAGATGAAACATTGGAAGCATTCTTTATGTTACCTATACCAATGCATCCTGGCTTGATTCCAGAATTGAAGACTGGTCTTGATAGATGTCTTCAATACTATGTATCGAAGGCCAAGACTGGCTGTGGAACCCGAATTACCTTCATTCCCACTATGCCTGCTTTGACTAGATGTTCAGCGGGATCAAAATTTCATGGTGTATTCAAGAAGAAGGAAAGGTCACATATAAACCAGAGGAGGAAATCTCAGGTTGGAAGTACAAATGGGGATGGTTCGTTTGGGATTCCGCAACTTTGTGTTCGCATTAATACTCTGCAGCGTATTCGAATGGAGTTGGGAGTTTTTGAGAAGAGGATTGTTGCTCATCTTGGGAACTTGGAATCCACTCAAAAGGGAGATTTTGCGAATGGGATGGGCAAAATGTTTGAGCTGTCAGCATCTGCTTGTTTAGAAGGGATCCAACAACTTTGTGAGGCAACAGCTTACAAGGTTGTTTTCCATGAACTAAGCCATGTTCTTTGGGATGGCTTGTATAATGTGGATGCTCCCTCATGTAGAATTGAGCCTTTTCTTCAGGAACTCGAGCAATTTTTGGAGATTATCTCGTCAACAGTTCACGACAGAGTTAGAACGCGTCTTATTACGGATGTCATGAAAGCTTCTTTTGACGGGTTCTTGTTGGTTTTGCTAGCTGGAGGCCCTTCCCGCAGTTTCACACTCAGAGATTCTAACATAATAGAGGAGGATTTTAAATTTCTGACTGATTTGTTCTGGTCAAACGGGGATGGATTACCAGCTGATTTAATAGCTAAGTTGTCAACCACTGTTAAGGACATCCTTCCTCTCTACCGTACTGATACTGATAGCCTTATTGAAAAGTTCAAGCGCGCGACTCTGGAGAACTATGGCTCGTCTGCGAAGTCCCACCTGCCATTGCCTCCAACTTCCAATGAGTGGAATTCCAATGACCCAAATACACTTCTACGTGTCTTATGTCATAGGAATGATGAGACTGCAGCCAAGTTTCTGAAGAAGACTTACAACTTGCCTACAAAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

987

Amino Acids

111.13

Weight (kDa)

8.33

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_PATROL1 PF25761 588 - 986 6e-167 PATROL1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017964)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09950
prunus_persica Prupe.7G187700_v2.0.a1
pyrus_communis pycom02g08680
rosa_chinensis RchiOBHm_Chr2g0096741
rosa_laevigata RLG00000016666
rosa_roxburghii Rroxscaffold_2G00145360
rosa_rugosa Rorug02G0060400
rosa_samantha Rh2AG107600 Rh2BG110000 Rh2CG111900 Rh2DG111300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2838
AatII GACGTC 1 cut(s) 347
Acc36I ACCTGC 3 cut(s) 144, 1068, 2838
AccB1I GGYRCC 1 cut(s) 1524
AccB7I CCANNNNNTGG 1 cut(s) 2856
AccBSI CCGCTC 2 cut(s) 244, 689
AccI GTMKAC 2 cut(s) 198, 279
AccII CGCG 5 cut(s) 105, 1772, 2536, 2790, 2792
AccIII TCCGGA 1 cut(s) 702
AclWI GGATC 5 cut(s) 199, 1115, 2092, 2353, 2366
AcoI YGGCCR 1 cut(s) 1042
AcsI RAATTY 6 cut(s) 286, 622, 987, 2090, 2654, 2860
AcuI CTGAAG 5 cut(s) 314, 1770, 1826, 2459, 2951
AcvI CACGTG 1 cut(s) 1331
AcyI GRCGYC 2 cut(s) 344, 383
AfaI GTAC 6 cut(s) 817, 1272, 1629, 1635, 2161, 2755
AfiI CCNNNNNNNGG 7 cut(s) 151, 262, 282, 454, 483, 1087, 2856
AflIII ACRYGT 3 cut(s) 103, 2534, 2887
AhlI ACTAGT 1 cut(s) 1273
AjnI CCWGG 4 cut(s) 882, 962, 1248, 1951
AloI GAACNNNNNNTCC 2 cut(s) 2267, 2299
Alw21I GWGCWC 4 cut(s) 230, 438, 1584, 1770
Alw26I GTCTC 6 cut(s) 12, 752, 795, 1249, 1569, 2906
AlwI GGATC 5 cut(s) 199, 1115, 2092, 2353, 2366
AlwNI CAGNNNCTG 2 cut(s) 1387, 2342
Ama87I CYCGRG 1 cut(s) 2483
Aor13HI TCCGGA 1 cut(s) 702
AoxI GGCC 8 cut(s) 3, 160, 232, 720, 1042, 1479, 2013, 2599
ApaI GGGCCC 1 cut(s) 164
ApeKI GCWGC 6 cut(s) 506, 1070, 1558, 1643, 2218, 2918
ApoI RAATTY 6 cut(s) 286, 622, 987, 2090, 2654, 2860
ArsI GACNNNNNNTTYG 6 cut(s) 11, 43, 93, 125, 1049, 1081
AseI ATTAAT 1 cut(s) 2208
AspLEI GCGC 3 cut(s) 1413, 2790, 2792
AsuC2I CCSGG 2 cut(s) 125, 421
AsuHPI GGTGA 2 cut(s) 238, 265
AsuII TTCGAA 1 cut(s) 2227
AsuNHI GCTAGC 1 cut(s) 2590
AvaI CYCGRG 1 cut(s) 2483
AvaII GGWCC 4 cut(s) 181, 417, 445, 1505
BaeGI GKGCMC 1 cut(s) 164
BalI TGGCCA 1 cut(s) 1044
BamHI GGATCC 1 cut(s) 2358
BanI GGYRCC 1 cut(s) 1524
BanII GRGCYC 7 cut(s) 164, 230, 438, 493, 1403, 1584, 1770
BarI GAAGNNNNNNTAC 2 cut(s) 2924, 2956
BauI CACGAG 1 cut(s) 1292
BbrPI CACGTG 1 cut(s) 1331
BbsI GAAGAC 6 cut(s) 138, 288, 965, 1979, 1985, 2942
Bbv12I GWGCWC 4 cut(s) 230, 438, 1584, 1770
BbvI GCAGC 6 cut(s) 493, 1082, 1545, 1630, 2230, 2930
BceAI ACGGC 2 cut(s) 396, 652
BcgI CGANNNNNNTGC 2 cut(s) 2207, 2241
BciT130I CCWGG 4 cut(s) 884, 964, 1250, 1953
BciVI GTATCC 1 cut(s) 666
BcnI CCSGG 2 cut(s) 125, 421
BcoDI GTCTC 6 cut(s) 12, 752, 795, 1249, 1569, 2906
BcuI ACTAGT 1 cut(s) 1273
BfaI CTAG 5 cut(s) 209, 1274, 1835, 2070, 2591
BfmI CTRYAG 3 cut(s) 799, 2216, 2916
BfoI RGCGCY 1 cut(s) 1414
BfuAI ACCTGC 3 cut(s) 144, 1068, 2838
BfuI GTATCC 1 cut(s) 666
BglII AGATCT 1 cut(s) 1663
BisI GCNGC 6 cut(s) 507, 1071, 1559, 1644, 2219, 2919
BlpI GCTNAGC 1 cut(s) 1764
BlsI GCNGC 6 cut(s) 508, 1072, 1560, 1645, 2220, 2920
BmcAI AGTACT 2 cut(s) 1272, 1635
Bme1390I CCNGG 6 cut(s) 125, 421, 884, 964, 1250, 1953
Bme18I GGWCC 4 cut(s) 181, 417, 445, 1505
BmeT110I CYCGRG 1 cut(s) 2483
BmrFI CCNGG 6 cut(s) 125, 421, 884, 964, 1250, 1953
BmrI ACTGGG 3 cut(s) 286, 847, 1175
BmsI GCATC 7 cut(s) 175, 1093, 1221, 1722, 1957, 2348, 2434
BmtI GCTAGC 1 cut(s) 2594
BmuI ACTGGG 3 cut(s) 286, 847, 1175
BpiI GAAGAC 6 cut(s) 138, 288, 965, 1979, 1985, 2942
BpmI CTGGAG 3 cut(s) 572, 2616, 2819
Bpu1102I GCTNAGC 1 cut(s) 1764
Bpu14I TTCGAA 1 cut(s) 2227
BpuEI CTTGAG 1 cut(s) 1568
BpuMI CCSGG 2 cut(s) 125, 421
Bsa29I ATCGAT 1 cut(s) 1027
BsaAI YACGTR 3 cut(s) 716, 1331, 2888
BsaHI GRCGYC 2 cut(s) 344, 383
BsaJI CCNNGG 6 cut(s) 723, 735, 963, 973, 1248, 1249
BsaWI WCCGGW 2 cut(s) 282, 702
BsaXI ACNNNNNCTCC 2 cut(s) 1229, 1259
Bsc4I CCNNNNNNNGG 7 cut(s) 151, 262, 282, 454, 483, 1087, 2856
Bse3DI GCAATG 1 cut(s) 2834
BseAI TCCGGA 1 cut(s) 702
BseBI CCWGG 4 cut(s) 884, 964, 1250, 1953
BseCI ATCGAT 1 cut(s) 1027
BseDI CCNNGG 6 cut(s) 723, 735, 963, 973, 1248, 1249
BseLI CCNNNNNNNGG 7 cut(s) 151, 262, 282, 454, 483, 1087, 2856
BseMI GCAATG 1 cut(s) 2834
BseMII CTCAG 7 cut(s) 511, 877, 1167, 1394, 1755, 2162, 2635
BsePI GCGCGC 1 cut(s) 2788
BseRI GAGGAG 4 cut(s) 631, 681, 2152, 2657
BseSI GKGCMC 1 cut(s) 164
BseXI GCAGC 6 cut(s) 493, 1082, 1545, 1630, 2230, 2930
BsgI GTGCAG 1 cut(s) 1691
Bsh1236I CGCG 5 cut(s) 105, 1772, 2536, 2790, 2792
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 8 cut(s) 5, 162, 234, 722, 1044, 1481, 2015, 2601
BshNI GGYRCC 1 cut(s) 1524
BshVI ATCGAT 1 cut(s) 1027
BsiEI CGRYCG 1 cut(s) 129
BsiHKAI GWGCWC 4 cut(s) 230, 438, 1584, 1770
BsiHKCI CYCGRG 1 cut(s) 2483
BsiSI CCGG 6 cut(s) 125, 223, 231, 283, 420, 703
BslFI GGGAC 4 cut(s) 50, 167, 657, 2809
BslI CCNNNNNNNGG 7 cut(s) 151, 262, 282, 454, 483, 1087, 2856
BsmAI GTCTC 6 cut(s) 12, 752, 795, 1249, 1569, 2906
BsmBI CGTCTC 1 cut(s) 1569
BsmFI GGGAC 4 cut(s) 50, 167, 657, 2809
BsmI GAATGC 2 cut(s) 1561, 1922
BsnI GGCC 8 cut(s) 5, 162, 234, 722, 1044, 1481, 2015, 2601
BsoBI CYCGRG 1 cut(s) 2483
Bsp119I TTCGAA 1 cut(s) 2227
Bsp120I GGGCCC 1 cut(s) 160
Bsp1286I GDGCHC 7 cut(s) 164, 230, 438, 493, 1403, 1584, 1770
Bsp13I TCCGGA 1 cut(s) 702
Bsp1407I TGTACA 1 cut(s) 1627
Bsp143I GATC 9 cut(s) 204, 270, 803, 1120, 1137, 1355, 1663, 2084, 2358
Bsp1720I GCTNAGC 1 cut(s) 1764
Bsp19I CCATGG 1 cut(s) 735
Bsp68I TCGCGA 1 cut(s) 1772
BspANI GGCC 8 cut(s) 5, 162, 234, 722, 1044, 1481, 2015, 2601
BspCNI CTCAG 7 cut(s) 510, 876, 1168, 1393, 1756, 2161, 2634
BspDI ATCGAT 1 cut(s) 1027
BspEI TCCGGA 1 cut(s) 702
BspFNI CGCG 5 cut(s) 105, 1772, 2536, 2790, 2792
BspHI TCATGA 2 cut(s) 951, 2553
BspMAI CTGCAG 3 cut(s) 803, 2220, 2920
BspMI ACCTGC 3 cut(s) 144, 1068, 2838
BspOI GCTAGC 1 cut(s) 2594
BspPI GGATC 5 cut(s) 199, 1115, 2092, 2353, 2366
BspQI GCTCTTC 1 cut(s) 921
BspT104I TTCGAA 1 cut(s) 2227
BspT107I GGYRCC 1 cut(s) 1524
BsrBI CCGCTC 2 cut(s) 244, 689
BsrDI GCAATG 1 cut(s) 2834
BsrGI TGTACA 1 cut(s) 1627
BssECI CCNNGG 6 cut(s) 723, 735, 963, 973, 1248, 1249
BssHII GCGCGC 1 cut(s) 2788
BssMI GATC 9 cut(s) 204, 270, 803, 1120, 1137, 1355, 1663, 2084, 2358
BssNI GRCGYC 2 cut(s) 344, 383
BssSI CACGAG 1 cut(s) 1292
BssT1I CCWWGG 3 cut(s) 723, 735, 973
Bst2BI CACGAG 1 cut(s) 1292
Bst2UI CCWGG 4 cut(s) 884, 964, 1250, 1953
Bst4CI ACNGT 9 cut(s) 196, 277, 417, 820, 1199, 1627, 2515, 2728, 2753
Bst6I CTCTTC 4 cut(s) 921, 1301, 1511, 2246
BstACI GRCGYC 2 cut(s) 344, 383
BstAUI TGTACA 1 cut(s) 1627
BstBAI YACGTR 3 cut(s) 716, 1331, 2888
BstBI TTCGAA 1 cut(s) 2227
BstC8I GCNNGC 7 cut(s) 164, 220, 799, 1770, 2061, 2592, 2790
BstDSI CCRYGG 1 cut(s) 735
BstFNI CGCG 5 cut(s) 105, 1772, 2536, 2790, 2792
BstH2I RGCGCY 1 cut(s) 1414
BstHHI GCGC 3 cut(s) 1413, 2790, 2792
BstKTI GATC 9 cut(s) 207, 273, 806, 1123, 1140, 1358, 1666, 2087, 2361
BstMAI GTCTC 6 cut(s) 12, 752, 795, 1249, 1569, 2906
BstMBI GATC 9 cut(s) 204, 270, 803, 1120, 1137, 1355, 1663, 2084, 2358
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 8 cut(s) 359, 553, 562, 1760, 1769, 1880, 2607, 2956
BstNI CCWGG 4 cut(s) 884, 964, 1250, 1953
BstNSI RCATGY 1 cut(s) 834
BstSCI CCNGG 6 cut(s) 123, 419, 882, 962, 1248, 1951
BstSFI CTRYAG 3 cut(s) 799, 2216, 2916
BstSLI GKGCMC 1 cut(s) 164
BstUI CGCG 5 cut(s) 105, 1772, 2536, 2790, 2792
BstV1I GCAGC 6 cut(s) 493, 1082, 1545, 1630, 2230, 2930
BstV2I GAAGAC 6 cut(s) 138, 288, 965, 1979, 1985, 2942
BstX2I RGATCY 4 cut(s) 204, 1120, 1663, 2358
BstYI RGATCY 4 cut(s) 204, 1120, 1663, 2358
Bsu15I ATCGAT 1 cut(s) 1027
BsuI GTATCC 1 cut(s) 666
BsuRI GGCC 8 cut(s) 5, 162, 234, 722, 1044, 1481, 2015, 2601
BsuTUI ATCGAT 1 cut(s) 1027
BtgI CCRYGG 1 cut(s) 735
BtsIMutI CAGTG 5 cut(s) 835, 1212, 1515, 1612, 2724
BtuMI TCGCGA 1 cut(s) 1772
BveI ACCTGC 3 cut(s) 144, 1068, 2838
Cac8I GCNNGC 7 cut(s) 164, 220, 799, 1770, 2061, 2592, 2790
CaiI CAGNNNCTG 2 cut(s) 1387, 2342
CciI TCATGA 2 cut(s) 951, 2553
CfoI GCGC 3 cut(s) 1413, 2790, 2792
ClaI ATCGAT 1 cut(s) 1027
CpoI CGGWCCG 1 cut(s) 417
CseI GACGC 3 cut(s) 94, 372, 2525
Csp6I GTAC 6 cut(s) 816, 1271, 1628, 1634, 2160, 2754
CspI CGGWCCG 1 cut(s) 417
CviQI GTAC 6 cut(s) 816, 1271, 1628, 1634, 2160, 2754
DpnI GATC 9 cut(s) 206, 272, 805, 1122, 1139, 1357, 1665, 2086, 2360
DpnII GATC 9 cut(s) 204, 270, 803, 1120, 1137, 1355, 1663, 2084, 2358
DraI TTTAAA 1 cut(s) 2653
EaeI YGGCCR 1 cut(s) 1042
Eam1104I CTCTTC 4 cut(s) 921, 1301, 1511, 2246
EarI CTCTTC 4 cut(s) 921, 1301, 1511, 2246
EciI GGCGGA 1 cut(s) 1103
Ecl136II GAGCTC 4 cut(s) 228, 436, 1582, 1768
Eco130I CCWWGG 3 cut(s) 723, 735, 973
Eco24I GRGCYC 7 cut(s) 164, 230, 438, 493, 1403, 1584, 1770
Eco32I GATATC 1 cut(s) 1285
Eco47I GGWCC 4 cut(s) 181, 417, 445, 1505
Eco53kI GAGCTC 4 cut(s) 228, 436, 1582, 1768
Eco57I CTGAAG 5 cut(s) 314, 1770, 1826, 2459, 2951
Eco72I CACGTG 1 cut(s) 1331
Eco88I CYCGRG 1 cut(s) 2483
EcoICRI GAGCTC 4 cut(s) 228, 436, 1582, 1768
EcoO109I RGGNCCY 2 cut(s) 720, 2600
EcoRI GAATTC 2 cut(s) 286, 2860
EcoRII CCWGG 4 cut(s) 882, 962, 1248, 1951
EcoRV GATATC 1 cut(s) 1285
EcoT14I CCWWGG 3 cut(s) 723, 735, 973
EcoT22I ATGCAT 1 cut(s) 1950
EcoT38I GRGCYC 7 cut(s) 164, 230, 438, 493, 1403, 1584, 1770
ErhI CCWWGG 3 cut(s) 723, 735, 973
Esp3I CGTCTC 1 cut(s) 1569
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FaqI GGGAC 4 cut(s) 50, 167, 657, 2809
FauI CCCGC 6 cut(s) 171, 264, 435, 1703, 2074, 2615
FauNDI CATATG 1 cut(s) 663
FblI GTMKAC 2 cut(s) 198, 279
Fnu4HI GCNGC 6 cut(s) 507, 1071, 1559, 1644, 2219, 2919
FriOI GRGCYC 7 cut(s) 164, 230, 438, 493, 1403, 1584, 1770
Fsp4HI GCNGC 6 cut(s) 507, 1071, 1559, 1644, 2219, 2919
FspBI CTAG 5 cut(s) 209, 1274, 1835, 2070, 2591
GlaI GCGC 3 cut(s) 1412, 2789, 2791
GluI GCNGC 6 cut(s) 507, 1071, 1559, 1644, 2219, 2919
GsuI CTGGAG 3 cut(s) 572, 2616, 2819
HaeII RGCGCY 1 cut(s) 1414
HaeIII GGCC 8 cut(s) 5, 162, 234, 722, 1044, 1481, 2015, 2601
HapII CCGG 6 cut(s) 125, 223, 231, 283, 420, 703
HgaI GACGC 3 cut(s) 94, 372, 2525
HhaI GCGC 3 cut(s) 1413, 2790, 2792
Hin1I GRCGYC 2 cut(s) 344, 383
Hin6I GCGC 3 cut(s) 1411, 2788, 2790
HinP1I GCGC 3 cut(s) 1411, 2788, 2790
HincII GTYRAC 5 cut(s) 1342, 1614, 1819, 2511, 2721
HindII GTYRAC 5 cut(s) 1342, 1614, 1819, 2511, 2721
HindIII AAGCTT 2 cut(s) 606, 2559
HpaII CCGG 6 cut(s) 125, 223, 231, 283, 420, 703
HphI GGTGA 2 cut(s) 238, 265
Hpy99I CGWCG 2 cut(s) 110, 388
HpyCH4III ACNGT 9 cut(s) 196, 277, 417, 820, 1199, 1627, 2515, 2728, 2753
HpyCH4IV ACGT 4 cut(s) 344, 715, 1330, 2887
HpyF10VI GCNNNNNNNGC 8 cut(s) 359, 553, 562, 1760, 1769, 1880, 2607, 2956
HpySE526I ACGT 4 cut(s) 344, 715, 1330, 2887
Hsp92I GRCGYC 2 cut(s) 344, 383
HspAI GCGC 3 cut(s) 1411, 2788, 2790
Kpn2I TCCGGA 1 cut(s) 702
Kzo9I GATC 9 cut(s) 204, 270, 803, 1120, 1137, 1355, 1663, 2084, 2358
LguI GCTCTTC 1 cut(s) 921
Lsp1109I GCAGC 6 cut(s) 493, 1082, 1545, 1630, 2230, 2930
LweI GCATC 7 cut(s) 175, 1093, 1221, 1722, 1957, 2348, 2434
MaeI CTAG 5 cut(s) 209, 1274, 1835, 2070, 2591
MaeII ACGT 4 cut(s) 344, 715, 1330, 2887
MaeIII GTNAC 6 cut(s) 324, 558, 1033, 1331, 1932, 2121
MalI GATC 9 cut(s) 206, 272, 805, 1122, 1139, 1357, 1665, 2086, 2360
MbiI CCGCTC 2 cut(s) 244, 689
MboI GATC 9 cut(s) 204, 270, 803, 1120, 1137, 1355, 1663, 2084, 2358
MflI RGATCY 4 cut(s) 204, 1120, 1663, 2358
MhlI GDGCHC 7 cut(s) 164, 230, 438, 493, 1403, 1584, 1770
MlsI TGGCCA 1 cut(s) 1044
MluI ACGCGT 2 cut(s) 103, 2534
MluNI TGGCCA 1 cut(s) 1044
MlyI GAGTC 5 cut(s) 72, 170, 1235, 1609, 2788
Mox20I TGGCCA 1 cut(s) 1044
Mph1103I ATGCAT 1 cut(s) 1950
MroI TCCGGA 1 cut(s) 702
MscI TGGCCA 1 cut(s) 1044
MseI TTAA 4 cut(s) 2208, 2652, 2705, 2730
MslI CAYNNNNRTG 2 cut(s) 1029, 2904
Msp20I TGGCCA 1 cut(s) 1044
MspA1I CMGCKG 2 cut(s) 2081, 2699
MspI CCGG 6 cut(s) 125, 223, 231, 283, 420, 703
MspR9I CCNGG 6 cut(s) 125, 421, 884, 964, 1250, 1953
Mva1269I GAATGC 2 cut(s) 1561, 1922
MvaI CCWGG 4 cut(s) 884, 964, 1250, 1953
MvnI CGCG 5 cut(s) 105, 1772, 2536, 2790, 2792
MwoI GCNNNNNNNGC 8 cut(s) 359, 553, 562, 1760, 1769, 1880, 2607, 2956
NciI CCSGG 2 cut(s) 125, 421
NcoI CCATGG 1 cut(s) 735
NdeI CATATG 1 cut(s) 663
NdeII GATC 9 cut(s) 204, 270, 803, 1120, 1137, 1355, 1663, 2084, 2358
NheI GCTAGC 1 cut(s) 2590
NmuCI GTSAC 3 cut(s) 324, 1331, 2121
NruI TCGCGA 1 cut(s) 1772
NsiI ATGCAT 1 cut(s) 1950
NspI RCATGY 1 cut(s) 834
NspV TTCGAA 1 cut(s) 2227
PaeR7I CTCGAG 1 cut(s) 2483
PagI TCATGA 2 cut(s) 951, 2553
PaqCI CACCTGC 1 cut(s) 2838
PasI CCCWGGG 1 cut(s) 1249
PauI GCGCGC 1 cut(s) 2788
PciSI GCTCTTC 1 cut(s) 921
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 350
PctI GAATGC 2 cut(s) 1561, 1922
PflMI CCANNNNNTGG 1 cut(s) 2856
PfoI TCCNGGA 1 cut(s) 882
PkrI GCNGC 6 cut(s) 508, 1072, 1560, 1645, 2220, 2920
PleI GAGTC 5 cut(s) 71, 170, 1234, 1609, 2788
PmaCI CACGTG 1 cut(s) 1331
PmlI CACGTG 1 cut(s) 1331
PpsI GAGTC 5 cut(s) 71, 170, 1234, 1609, 2788
Ppu21I YACGTR 3 cut(s) 716, 1331, 2888
PshBI ATTAAT 1 cut(s) 2208
Psp124BI GAGCTC 4 cut(s) 230, 438, 1584, 1770
Psp6I CCWGG 4 cut(s) 882, 962, 1248, 1951
PspCI CACGTG 1 cut(s) 1331
PspGI CCWGG 4 cut(s) 882, 962, 1248, 1951
PspOMI GGGCCC 1 cut(s) 160
PspXI VCTCGAGB 1 cut(s) 2483
PsrI GAACNNNNNNTAC 2 cut(s) 2023, 2055
PstI CTGCAG 3 cut(s) 803, 2220, 2920
PstNI CAGNNNCTG 2 cut(s) 1387, 2342
PsuI RGATCY 4 cut(s) 204, 1120, 1663, 2358
PteI GCGCGC 1 cut(s) 2788
PvuII CAGCTG 1 cut(s) 2699
RruI TCGCGA 1 cut(s) 1772
RsaI GTAC 6 cut(s) 817, 1272, 1629, 1635, 2161, 2755
RsaNI GTAC 6 cut(s) 816, 1271, 1628, 1634, 2160, 2754
RseI CAYNNNNRTG 2 cut(s) 1029, 2904
Rsr2I CGGWCCG 1 cut(s) 417
RsrII CGGWCCG 1 cut(s) 417
SacI GAGCTC 4 cut(s) 230, 438, 1584, 1770
SapI GCTCTTC 1 cut(s) 921
SaqAI TTAA 4 cut(s) 2208, 2652, 2705, 2730
SatI GCNGC 6 cut(s) 507, 1071, 1559, 1644, 2219, 2919
Sau3AI GATC 9 cut(s) 204, 270, 803, 1120, 1137, 1355, 1663, 2084, 2358
ScaI AGTACT 2 cut(s) 1272, 1635
SchI GAGTC 5 cut(s) 72, 170, 1235, 1609, 2788
ScrFI CCNGG 6 cut(s) 125, 421, 884, 964, 1250, 1953
SduI GDGCHC 7 cut(s) 164, 230, 438, 493, 1403, 1584, 1770
SfaNI GCATC 7 cut(s) 175, 1093, 1221, 1722, 1957, 2348, 2434
SfcI CTRYAG 3 cut(s) 799, 2216, 2916
Sfr274I CTCGAG 1 cut(s) 2483
SfuI TTCGAA 1 cut(s) 2227
SinI GGWCC 4 cut(s) 181, 417, 445, 1505
SlaI CTCGAG 1 cut(s) 2483
SmiMI CAYNNNNRTG 2 cut(s) 1029, 2904
SmlI CTYRAG 2 cut(s) 1583, 2483
SmoI CTYRAG 2 cut(s) 1583, 2483
SpeI ACTAGT 1 cut(s) 1273
SspI AATATT 1 cut(s) 1222
SspMI CTAG 5 cut(s) 209, 1274, 1835, 2070, 2591
SstI GAGCTC 4 cut(s) 230, 438, 1584, 1770
StyD4I CCNGG 6 cut(s) 123, 419, 882, 962, 1248, 1951
StyI CCWWGG 3 cut(s) 723, 735, 973
TaaI ACNGT 9 cut(s) 196, 277, 417, 820, 1199, 1627, 2515, 2728, 2753
TaiI ACGT 4 cut(s) 347, 718, 1333, 2890
TaqI TCGA 8 cut(s) 129, 1027, 1229, 1683, 1743, 2009, 2227, 2484
TaqII GACCGA 1 cut(s) 485
TatI WGTACW 4 cut(s) 1270, 1627, 1633, 2159
Tru1I TTAA 4 cut(s) 2208, 2652, 2705, 2730
Tru9I TTAA 4 cut(s) 2208, 2652, 2705, 2730
TscAI CASTG 5 cut(s) 842, 1219, 1515, 1612, 2731
TseFI GTSAC 3 cut(s) 324, 1331, 2121
TseI GCWGC 6 cut(s) 506, 1070, 1558, 1643, 2218, 2918
Tsp45I GTSAC 3 cut(s) 324, 1331, 2121
TspDTI ATGAA 9 cut(s) 618, 921, 968, 1013, 1923, 2035, 2084, 2418, 2570
TspGWI ACGGA 3 cut(s) 843, 1441, 2561
TspRI CASTG 5 cut(s) 842, 1219, 1515, 1612, 2731
Van91I CCANNNNNTGG 1 cut(s) 2856
VpaK11BI GGWCC 4 cut(s) 181, 417, 445, 1505
VspI ATTAAT 1 cut(s) 2208
XapI RAATTY 6 cut(s) 286, 622, 987, 2090, 2654, 2860
XceI RCATGY 1 cut(s) 834
XcmI CCANNNNNNNNNTGG 2 cut(s) 842, 1081
XhoI CTCGAG 1 cut(s) 2483
XmiI GTMKAC 2 cut(s) 198, 279
XspI CTAG 5 cut(s) 209, 1274, 1835, 2070, 2591
ZraI GACGTC 1 cut(s) 345
ZrmI AGTACT 2 cut(s) 1272, 1635
Zsp2I ATGCAT 1 cut(s) 1950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.