Rorug02G0446600

membrane protein At3g27390

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013566
Physical Location & Seq
Reverse (-)
57311720 .. 57312841
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0446600.1

Sequence Viewer

Length: 387 bp
ATGGCGAAGACAGGGAAGCTGACGAAGCTCAAGTCGGCCATAAAGAGATGGCCCTCCTTTACCAAACTGGCTCGCACCACAAGCTCCATAGCCGCCACAAACGACGATTCGTCCTCCGACCAATACGTCGACAACGACAGGGCAGTGGTCTCCAAGCAGCTTCACGCCGTCTACGTGGGGAAGTCGCGGCGCCGGTACATGGTGAGCTCCGACGTGGTGGAGCACCCCATAATTCAAGAGCTGGTGGACAAGTCTTGTGAGGAAGAAGAAGGGGTTGTGGTGGTGACTTGCGAGGTCGTGCTTTTCGAGCACTTGCTGTGGATGCTTGAGAACGCCGAGACTCAGTTGGGTTCCATGGACGAGCTGGTTGAGTTCTACACTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

128

Amino Acids

14.46

Weight (kDa)

5.19

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 10 - 109 1.5e-20 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AccB1I GGYRCC 1 cut(s) 189
AccI GTMKAC 2 cut(s) 129, 171
AccII CGCG 1 cut(s) 187
AciI CCGC 2 cut(s) 93, 187
AcoI YGGCCR 1 cut(s) 36
AcyI GRCGYC 1 cut(s) 190
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 1 cut(s) 199
AgsI TTSAA 1 cut(s) 236
AhdI GACNNNNNGTC 1 cut(s) 109
AjiI CACGTC 1 cut(s) 214
AluBI AGCT 7 cut(s) 19, 28, 84, 160, 207, 241, 364
AluI AGCT 7 cut(s) 19, 28, 84, 160, 207, 241, 364
Alw21I GWGCWC 3 cut(s) 209, 225, 312
Alw26I GTCTC 2 cut(s) 154, 332
AoxI GGCC 2 cut(s) 36, 50
ApeKI GCWGC 1 cut(s) 157
ArsI GACNNNNNNTTYG 2 cut(s) 17, 49
AspLEI GCGC 1 cut(s) 192
AspS9I GGNCC 1 cut(s) 51
AsuHPI GGTGA 2 cut(s) 214, 295
BanI GGYRCC 1 cut(s) 189
BanII GRGCYC 1 cut(s) 209
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 3 cut(s) 209, 225, 312
BbvI GCAGC 1 cut(s) 169
BccI CCATC 1 cut(s) 42
BceAI ACGGC 1 cut(s) 152
BcoDI GTCTC 2 cut(s) 154, 332
BfoI RGCGCY 1 cut(s) 193
BisI GCNGC 3 cut(s) 93, 158, 188
BlsI GCNGC 3 cut(s) 94, 159, 189
BmeRI GACNNNNNGTC 1 cut(s) 109
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 51
BmiI GGNNCC 2 cut(s) 191, 352
BmsI GCATC 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 14
BpuEI CTTGAG 2 cut(s) 14, 347
BsaAI YACGTR 1 cut(s) 175
BsaHI GRCGYC 1 cut(s) 190
BsaI GGTCTC 1 cut(s) 154
BsaJI CCNNGG 1 cut(s) 354
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse118I RCCGGY 1 cut(s) 192
Bse1I ACTGG 1 cut(s) 72
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 1 cut(s) 327
BseLI CCNNNNNNNGG 1 cut(s) 199
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 1 cut(s) 72
BseXI GCAGC 1 cut(s) 169
Bsh1236I CGCG 1 cut(s) 187
BshFI GGCC 2 cut(s) 38, 52
BshNI GGYRCC 1 cut(s) 189
BsiHKAI GWGCWC 3 cut(s) 209, 225, 312
BsiSI CCGG 1 cut(s) 193
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 2 cut(s) 154, 332
BsnI GGCC 2 cut(s) 38, 52
Bso31I GGTCTC 1 cut(s) 154
Bsp1286I GDGCHC 3 cut(s) 209, 225, 312
Bsp19I CCATGG 1 cut(s) 354
BspACI CCGC 2 cut(s) 93, 187
BspANI GGCC 2 cut(s) 38, 52
BspCNI CTCAG 1 cut(s) 355
BspFNI CGCG 1 cut(s) 187
BspLI GGNNCC 2 cut(s) 191, 352
BspT107I GGYRCC 1 cut(s) 189
BspTNI GGTCTC 1 cut(s) 154
BsrFI RCCGGY 1 cut(s) 192
BsrI ACTGG 1 cut(s) 72
BssAI RCCGGY 1 cut(s) 192
BssECI CCNNGG 1 cut(s) 354
BssNI GRCGYC 1 cut(s) 190
BssT1I CCWWGG 1 cut(s) 354
BstACI GRCGYC 1 cut(s) 190
BstBAI YACGTR 1 cut(s) 175
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 1 cut(s) 342
BstDSI CCRYGG 1 cut(s) 354
BstF5I GGATG 1 cut(s) 327
BstFNI CGCG 1 cut(s) 187
BstH2I RGCGCY 1 cut(s) 193
BstHHI GCGC 1 cut(s) 192
BstMAI GTCTC 2 cut(s) 154, 332
BstMWI GCNNNNNNNGC 4 cut(s) 25, 81, 307, 322
BstUI CGCG 1 cut(s) 187
BstV1I GCAGC 1 cut(s) 169
BstV2I GAAGAC 1 cut(s) 14
BsuRI GGCC 2 cut(s) 38, 52
BtgI CCRYGG 1 cut(s) 354
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 1 cut(s) 327
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 1 cut(s) 150
Cac8I GCNNGC 1 cut(s) 73
CfoI GCGC 1 cut(s) 192
Cfr10I RCCGGY 1 cut(s) 192
Cfr13I GGNCC 1 cut(s) 51
Csp6I GTAC 1 cut(s) 196
CviAII CATG 2 cut(s) 199, 355
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 342
DinI GGCGCC 1 cut(s) 191
DrdI GACNNNNNNGTC 1 cut(s) 125
DriI GACNNNNNGTC 1 cut(s) 109
DseDI GACNNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 1 cut(s) 36
Eam1105I GACNNNNNGTC 1 cut(s) 109
Ecl136II GAGCTC 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 209
Eco31I GGTCTC 1 cut(s) 154
Eco53kI GAGCTC 1 cut(s) 207
EcoICRI GAGCTC 1 cut(s) 207
EcoT14I CCWWGG 1 cut(s) 354
EcoT38I GRGCYC 1 cut(s) 209
EgeI GGCGCC 1 cut(s) 191
EheI GGCGCC 1 cut(s) 191
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 2 cut(s) 202, 358
FaiI YATR 5 cut(s) 41, 89, 200, 230, 356
FatI CATG 2 cut(s) 198, 354
FblI GTMKAC 2 cut(s) 129, 171
Fnu4HI GCNGC 3 cut(s) 93, 158, 188
FokI GGATG 1 cut(s) 334
FriOI GRGCYC 1 cut(s) 209
Fsp4HI GCNGC 3 cut(s) 93, 158, 188
GlaI GCGC 1 cut(s) 191
GluI GCNGC 3 cut(s) 93, 158, 188
HaeII RGCGCY 1 cut(s) 193
HaeIII GGCC 2 cut(s) 38, 52
HapII CCGG 1 cut(s) 193
HhaI GCGC 1 cut(s) 192
Hin1I GRCGYC 1 cut(s) 190
Hin1II CATG 2 cut(s) 202, 358
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 2 cut(s) 107, 340
HpaII CCGG 1 cut(s) 193
HphI GGTGA 2 cut(s) 214, 295
Hpy166II GTNNAC 3 cut(s) 130, 172, 247
Hpy188I TCNGA 2 cut(s) 118, 211
Hpy188III TCNNGA 1 cut(s) 236
Hpy8I GTNNAC 3 cut(s) 130, 172, 247
Hpy99I CGWCG 3 cut(s) 107, 131, 215
HpyAV CCTTC 1 cut(s) 263
HpyCH4IV ACGT 3 cut(s) 126, 174, 213
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 81, 307, 322
HpyF3I CTNAG 1 cut(s) 342
HpySE526I ACGT 3 cut(s) 126, 174, 213
Hsp92I GRCGYC 1 cut(s) 190
Hsp92II CATG 2 cut(s) 202, 358
HspAI GCGC 1 cut(s) 190
KasI GGCGCC 1 cut(s) 189
LmnI GCTCC 3 cut(s) 89, 212, 220
LpnPI CCDG 5 cut(s) 53, 124, 206, 227, 350
Lsp1109I GCAGC 1 cut(s) 169
LweI GCATC 1 cut(s) 312
MaeII ACGT 3 cut(s) 126, 174, 213
MaeIII GTNAC 1 cut(s) 283
MboII GAAGA 3 cut(s) 19, 275, 278
MhlI GDGCHC 3 cut(s) 209, 225, 312
MluCI AATT 1 cut(s) 231
Mly113I GGCGCC 1 cut(s) 190
MlyI GAGTC 1 cut(s) 334
MmeI TCCRAC 2 cut(s) 141, 234
MnlI CCTC 4 cut(s) 64, 124, 253, 286
MspI CCGG 1 cut(s) 193
MvnI CGCG 1 cut(s) 187
MwoI GCNNNNNNNGC 4 cut(s) 25, 81, 307, 322
NarI GGCGCC 1 cut(s) 190
NcoI CCATGG 1 cut(s) 354
NlaIII CATG 2 cut(s) 202, 358
NlaIV GGNNCC 2 cut(s) 191, 352
NmeAIII GCCGAG 1 cut(s) 361
NmuCI GTSAC 1 cut(s) 283
PcsI WCGNNNNNNNCGW 3 cut(s) 132, 171, 303
PfeI GAWTC 1 cut(s) 107
PkrI GCNGC 3 cut(s) 94, 159, 189
PleI GAGTC 1 cut(s) 334
PluTI GGCGCC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 334
Ppu21I YACGTR 1 cut(s) 175
Psp124BI GAGCTC 1 cut(s) 209
PspN4I GGNNCC 2 cut(s) 191, 352
PspPI GGNCC 1 cut(s) 51
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SacI GAGCTC 1 cut(s) 209
SalI GTCGAC 1 cut(s) 128
SatI GCNGC 3 cut(s) 93, 158, 188
Sau96I GGNCC 1 cut(s) 51
SchI GAGTC 1 cut(s) 334
SduI GDGCHC 3 cut(s) 209, 225, 312
SfaNI GCATC 1 cut(s) 312
SfoI GGCGCC 1 cut(s) 191
SmlI CTYRAG 2 cut(s) 29, 326
SmoI CTYRAG 2 cut(s) 29, 326
Sse9I AATT 1 cut(s) 231
SsiI CCGC 2 cut(s) 93, 187
SspDI GGCGCC 1 cut(s) 189
SstI GAGCTC 1 cut(s) 209
StyI CCWWGG 1 cut(s) 354
TaiI ACGT 3 cut(s) 129, 177, 216
TaqI TCGA 2 cut(s) 129, 306
TasI AATT 1 cut(s) 231
TauI GCSGC 2 cut(s) 95, 190
TfiI GAWTC 1 cut(s) 107
TscAI CASTG 1 cut(s) 150
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 1 cut(s) 157
Tsp45I GTSAC 1 cut(s) 283
TspRI CASTG 1 cut(s) 150
XcmI CCANNNNNNNNNTGG 1 cut(s) 361
XmiI GTMKAC 2 cut(s) 129, 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.