Rorug04G0334300

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008280
Physical Location & Seq
Reverse (-)
50858368 .. 50860783
2416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0334300.1

Sequence Viewer

Length: 1509 bp
ATGGATTTCTTGAATTGTATAATAGGTCTTTGTTTTGCCTGGATCACCAGCCAAGCGTTCTATCGTTTTGCAAGAAGAAGCAAAGCTATACCCAGAAAGCTTCTTCCACCAGGACCAAAGCCATTTCCACTGGTTGGGAATCTGTTTGAGCTTGGAGACAAACCCCACCTCTCACTTACTAATCTTTCCCAACGCTATGGCCCCATCATAAGTTTGCAACTCGGCCGATTAACCACAGTAGTCATCTCTTCACCAACTCTGGCAAAAGAAATCCTCCGAACCCATGACCAAGTCTTCTGCAACCGACCCATCCGAGATGGAATCCACGCCTGCAAACACAGCGAGTACAGCATGGCCTGGATTCCTGTGTCAGCAAGATGGAGAAATCTTCGCAAAATATGCAACTTTCAATTGTTTTCCCCCAAAGTTCTTGATGCCAACAAAGCCAACCGACGCGTAAAGGTGCAAAAGCTCATCGATAATGTCAATGAAAGCATGAGGGCTGGTGAGGCAGTAGATATCGGAAGGGCTGCATTTACAACCGCGCTCAATTTGTTGTCGCAGACTATCTTCTCTGTGGATTTAGCTGACCCCAGTAGTGAGACAGCTAGGGAGTTCAAGGAGACTGTTTGGGGTATGTTTGAAGAGACAGGGAAACCAAACTTGGCGGACTATTTTCCTTTGCTTAGGAAGCTTGACCCCCAAGGCATAAGGTGGCGCTTGACCTATCACTACCAGAAAATGATAGAGATCTTTGATCGAATGATCCATCAAAGGCTGGAATCAAGAAAAGGGGACAGTTATATCACAACCAATGATATGTTGGATACACTTTTGAACATCAGTAAAGAGAAAATGGAGGACATGGACATGCTCGAAACACAACATTTGTTCCTGGATTTATTTGCTGCCGGCACGGACACAAGTTCAGCCACATTGGAATGGGCAATGGCCGAGCTACTACGCAACCCAAAAATTCTTTCAGAAGCTCAAGCGGAACTCCAGCAAGTGATTGGAAAGGGGAAAGTAGTTGAGGAATCAGACATTGCTCGACTCCCTTACTTACAAGCAATAATCAAAGAAACATTTCGGGTGCACCCAACAGTTCCATTGCTACTTCCCCGGAAAGCAGAAGCAAACATAGAAATTGGCGGGTATGTAATCCCGAAGGGTGCTCAAGTTCTAATAAATGTTTGGGCCATAGGTAGAGACCCCATCACTTGGGAAAACCCAAATCTGTTTAAGCCGGAGAGGTTCTTGGGATTAGAAAATCAAATTGATGTTATGGGAAAAAACTTCGAGCTTACTCCGTTTGGTGGTGGGAGAAGAATATGTCCCGGACTTCCATTGGCAATAAGAATGTTACACTTGATGCTGGGTTCACTAATTAACTGCTTTGATTGGAACCTTGAAGATGGAGTTGTACCCGAGACTATGAACATGGCAGAGAAGTTTGGCCTTACCCTACAAATGGCACAGCCACTAAGAGCTGTGCCCAAGAAGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0007155 GO:0008150 GO:0012505 GO:0022610 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

502

Amino Acids

57.0

Weight (kDa)

8.91

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 36 - 487 1.3e-104 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 134, 1221
AccII CGCG 2 cut(s) 456, 545
AciI CCGC 4 cut(s) 543, 668, 995, 1152
AclWI GGATC 2 cut(s) 50, 760
AcoI YGGCCR 2 cut(s) 223, 951
AcsI RAATTY 1 cut(s) 975
AfaI GTAC 2 cut(s) 347, 1425
AfiI CCNNNNNNNGG 4 cut(s) 134, 1221, 1316, 1471
AflIII ACRYGT 1 cut(s) 454
AgsI TTSAA 6 cut(s) 13, 410, 619, 644, 838, 1412
AjnI CCWGG 4 cut(s) 38, 109, 356, 894
AjuI GAANNNNNNNTTGG 4 cut(s) 647, 679, 964, 996
Alw21I GWGCWC 2 cut(s) 1098, 1177
Alw26I GTCTC 6 cut(s) 150, 596, 617, 641, 1203, 1424
Alw44I GTGCAC 1 cut(s) 1094
AlwI GGATC 2 cut(s) 50, 760
Ama87I CYCGRG 1 cut(s) 1427
AoxI GGCC 6 cut(s) 199, 223, 354, 951, 1197, 1456
ApaLI GTGCAC 1 cut(s) 1094
ApeKI GCWGC 2 cut(s) 530, 908
ApoI RAATTY 1 cut(s) 975
Asp700I GAANNNNTTC 1 cut(s) 1086
AspLEI GCGC 2 cut(s) 547, 720
AspS9I GGNCC 3 cut(s) 113, 200, 1197
AsuC2I CCSGG 2 cut(s) 1123, 1338
AsuHPI GGTGA 3 cut(s) 37, 243, 518
AvaI CYCGRG 1 cut(s) 1427
AvaII GGWCC 1 cut(s) 113
BaeGI GKGCMC 2 cut(s) 1098, 1497
BbsI GAAGAC 1 cut(s) 286
Bbv12I GWGCWC 2 cut(s) 1098, 1177
BbvI GCAGC 2 cut(s) 517, 895
BccI CCATC 7 cut(s) 212, 311, 317, 372, 777, 1223, 1409
BciT130I CCWGG 4 cut(s) 40, 111, 358, 896
BciVI GTATCC 1 cut(s) 820
BcnI CCSGG 2 cut(s) 1123, 1338
BcoDI GTCTC 6 cut(s) 150, 596, 617, 641, 1203, 1424
BfaI CTAG 1 cut(s) 609
BfoI RGCGCY 1 cut(s) 721
BfuI GTATCC 1 cut(s) 820
BglII AGATCT 1 cut(s) 750
BisI GCNGC 2 cut(s) 531, 909
BlsI GCNGC 2 cut(s) 532, 910
Bme1390I CCNGG 6 cut(s) 40, 111, 358, 896, 1123, 1338
Bme18I GGWCC 1 cut(s) 113
BmeT110I CYCGRG 1 cut(s) 1427
BmgT120I GGNCC 3 cut(s) 113, 200, 1197
BmiI GGNNCC 2 cut(s) 202, 1406
BmrFI CCNGG 6 cut(s) 40, 111, 358, 896, 1123, 1338
BmrI ACTGGG 1 cut(s) 588
BmsI GCATC 2 cut(s) 424, 1362
BmuI ACTGGG 1 cut(s) 588
BpiI GAAGAC 1 cut(s) 286
BpmI CTGGAG 1 cut(s) 986
Bpu10I CCTNAGC 1 cut(s) 686
BpuEI CTTGAG 2 cut(s) 975, 1161
BpuMI CCSGG 2 cut(s) 1123, 1338
Bsa29I ATCGAT 1 cut(s) 477
BsaBI GATNNNNATC 1 cut(s) 749
BsaI GGTCTC 1 cut(s) 1203
BsaJI CCNNGG 2 cut(s) 703, 1121
Bsc4I CCNNNNNNNGG 4 cut(s) 134, 1221, 1316, 1471
Bse118I RCCGGY 1 cut(s) 911
Bse1I ACTGG 2 cut(s) 135, 594
Bse3DI GCAATG 3 cut(s) 954, 1044, 1109
Bse8I GATNNNNATC 1 cut(s) 749
BseBI CCWGG 4 cut(s) 40, 111, 358, 896
BseCI ATCGAT 1 cut(s) 477
BseDI CCNNGG 2 cut(s) 703, 1121
BseGI GGATG 1 cut(s) 309
BseJI GATNNNNATC 1 cut(s) 749
BseLI CCNNNNNNNGG 4 cut(s) 134, 1221, 1316, 1471
BseMI GCAATG 3 cut(s) 954, 1044, 1109
BseNI ACTGG 2 cut(s) 135, 594
BseSI GKGCMC 2 cut(s) 1098, 1497
BseX3I CGGCCG 1 cut(s) 223
BseXI GCAGC 2 cut(s) 517, 895
BseYI CCCAGC 1 cut(s) 1375
Bsh1236I CGCG 2 cut(s) 456, 545
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 6 cut(s) 201, 225, 356, 953, 1199, 1458
BshVI ATCGAT 1 cut(s) 477
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 2 cut(s) 1098, 1177
BsiHKCI CYCGRG 1 cut(s) 1427
BsiSI CCGG 4 cut(s) 912, 1123, 1247, 1338
BslFI GGGAC 2 cut(s) 809, 1320
BslI CCNNNNNNNGG 4 cut(s) 134, 1221, 1316, 1471
BsmAI GTCTC 6 cut(s) 150, 596, 617, 641, 1203, 1424
BsmFI GGGAC 2 cut(s) 809, 1320
BsnI GGCC 6 cut(s) 201, 225, 356, 953, 1199, 1458
Bso31I GGTCTC 1 cut(s) 1203
BsoBI CYCGRG 1 cut(s) 1427
Bsp1286I GDGCHC 3 cut(s) 1098, 1177, 1497
Bsp143I GATC 4 cut(s) 42, 750, 757, 765
BspACI CCGC 4 cut(s) 543, 668, 995, 1152
BspANI GGCC 6 cut(s) 201, 225, 356, 953, 1199, 1458
BspDI ATCGAT 1 cut(s) 477
BspFNI CGCG 2 cut(s) 456, 545
BspLI GGNNCC 2 cut(s) 202, 1406
BspPI GGATC 2 cut(s) 50, 760
BspTNI GGTCTC 1 cut(s) 1203
BsrDI GCAATG 3 cut(s) 954, 1044, 1109
BsrFI RCCGGY 1 cut(s) 911
BsrI ACTGG 2 cut(s) 135, 594
BssAI RCCGGY 1 cut(s) 911
BssECI CCNNGG 2 cut(s) 703, 1121
BssMI GATC 4 cut(s) 42, 750, 757, 765
BssT1I CCWWGG 1 cut(s) 703
Bst2UI CCWGG 4 cut(s) 40, 111, 358, 896
Bst4CI ACNGT 4 cut(s) 238, 628, 800, 1105
Bst6I CTCTTC 2 cut(s) 253, 639
BstAPI GCANNNNNTGC 1 cut(s) 399
BstC8I GCNNGC 2 cut(s) 331, 913
BstDEI CTNAG 2 cut(s) 686, 1484
BstF5I GGATG 1 cut(s) 309
BstFNI CGCG 2 cut(s) 456, 545
BstH2I RGCGCY 1 cut(s) 721
BstHHI GCGC 2 cut(s) 547, 720
BstKTI GATC 4 cut(s) 45, 753, 760, 768
BstMAI GTCTC 6 cut(s) 150, 596, 617, 641, 1203, 1424
BstMBI GATC 4 cut(s) 42, 750, 757, 765
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 6 cut(s) 339, 348, 399, 443, 509, 691
BstNI CCWGG 4 cut(s) 40, 111, 358, 896
BstNSI RCATGY 1 cut(s) 874
BstSCI CCNGG 6 cut(s) 38, 109, 356, 894, 1121, 1336
BstSLI GKGCMC 2 cut(s) 1098, 1497
BstUI CGCG 2 cut(s) 456, 545
BstV1I GCAGC 2 cut(s) 517, 895
BstV2I GAAGAC 1 cut(s) 286
BstX2I RGATCY 1 cut(s) 750
BstXI CCANNNNNNTGG 1 cut(s) 197
BstYI RGATCY 1 cut(s) 750
BstZI CGGCCG 1 cut(s) 223
Bsu15I ATCGAT 1 cut(s) 477
BsuI GTATCC 1 cut(s) 820
BsuRI GGCC 6 cut(s) 201, 225, 356, 953, 1199, 1458
BsuTUI ATCGAT 1 cut(s) 477
BtsCI GGATG 1 cut(s) 309
BtsIMutI CAGTG 1 cut(s) 128
Cac8I GCNNGC 2 cut(s) 331, 913
CfoI GCGC 2 cut(s) 547, 720
Cfr10I RCCGGY 1 cut(s) 911
Cfr13I GGNCC 3 cut(s) 113, 200, 1197
ClaI ATCGAT 1 cut(s) 477
CseI GACGC 1 cut(s) 462
Csp6I GTAC 2 cut(s) 346, 1424
CviAII CATG 6 cut(s) 284, 352, 496, 865, 871, 1441
CviQI GTAC 2 cut(s) 346, 1424
DdeI CTNAG 2 cut(s) 686, 1484
DpnI GATC 4 cut(s) 44, 752, 759, 767
DpnII GATC 4 cut(s) 42, 750, 757, 765
EaeI YGGCCR 2 cut(s) 223, 951
EagI CGGCCG 1 cut(s) 223
Eam1104I CTCTTC 2 cut(s) 253, 639
EarI CTCTTC 2 cut(s) 253, 639
EciI GGCGGA 1 cut(s) 683
EclXI CGGCCG 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 703
Eco31I GGTCTC 1 cut(s) 1203
Eco32I GATATC 1 cut(s) 520
Eco47I GGWCC 1 cut(s) 113
Eco52I CGGCCG 1 cut(s) 223
Eco88I CYCGRG 1 cut(s) 1427
EcoRII CCWGG 4 cut(s) 38, 109, 356, 894
EcoRV GATATC 1 cut(s) 520
EcoT14I CCWWGG 1 cut(s) 703
ErhI CCWWGG 1 cut(s) 703
FaeI CATG 6 cut(s) 287, 355, 499, 868, 874, 1444
FaqI GGGAC 2 cut(s) 809, 1320
FatI CATG 6 cut(s) 283, 351, 495, 864, 870, 1440
FauI CCCGC 1 cut(s) 1145
Fnu4HI GCNGC 2 cut(s) 531, 909
FokI GGATG 1 cut(s) 296
Fsp4HI GCNGC 2 cut(s) 531, 909
FspBI CTAG 1 cut(s) 609
GlaI GCGC 2 cut(s) 546, 719
GluI GCNGC 2 cut(s) 531, 909
GsaI CCCAGC 1 cut(s) 1379
GsuI CTGGAG 1 cut(s) 986
HaeII RGCGCY 1 cut(s) 721
HaeIII GGCC 6 cut(s) 201, 225, 356, 953, 1199, 1458
HapII CCGG 4 cut(s) 912, 1123, 1247, 1338
HgaI GACGC 1 cut(s) 462
HhaI GCGC 2 cut(s) 547, 720
Hin1II CATG 6 cut(s) 287, 355, 499, 868, 874, 1444
Hin6I GCGC 2 cut(s) 545, 718
HinP1I GCGC 2 cut(s) 545, 718
HindIII AAGCTT 2 cut(s) 98, 692
HinfI GANTC 6 cut(s) 139, 321, 361, 782, 1037, 1053
HpaII CCGG 4 cut(s) 912, 1123, 1247, 1338
HphI GGTGA 3 cut(s) 37, 243, 518
Hpy166II GTNNAC 2 cut(s) 1096, 1382
Hpy188I TCNGA 5 cut(s) 278, 314, 524, 985, 1042
Hpy188III TCNNGA 4 cut(s) 10, 431, 786, 1165
Hpy8I GTNNAC 2 cut(s) 1096, 1382
Hpy99I CGWCG 1 cut(s) 456
HpyAV CCTTC 2 cut(s) 519, 1162
HpyCH4III ACNGT 4 cut(s) 238, 628, 800, 1105
HpyCH4V TGCA 8 cut(s) 71, 217, 300, 333, 402, 466, 533, 1096
HpyF10VI GCNNNNNNNGC 6 cut(s) 339, 348, 399, 443, 509, 691
HpyF3I CTNAG 2 cut(s) 686, 1484
Hsp92II CATG 6 cut(s) 287, 355, 499, 868, 874, 1444
HspAI GCGC 2 cut(s) 545, 718
KroI GCCGGC 1 cut(s) 911
KroNI GCCGGC 1 cut(s) 913
Kzo9I GATC 4 cut(s) 42, 750, 757, 765
Lsp1109I GCAGC 2 cut(s) 517, 895
LweI GCATC 2 cut(s) 424, 1362
MaeI CTAG 1 cut(s) 609
MaeIII GTNAC 1 cut(s) 1362
MalI GATC 4 cut(s) 44, 752, 759, 767
MboI GATC 4 cut(s) 42, 750, 757, 765
MboII GAAGA 9 cut(s) 87, 95, 240, 286, 380, 562, 656, 1338, 1424
MfeI CAATTG 1 cut(s) 410
MflI RGATCY 1 cut(s) 750
MhlI GDGCHC 3 cut(s) 1098, 1177, 1497
MluCI AATT 7 cut(s) 13, 410, 550, 975, 1146, 1275, 1386
MluI ACGCGT 1 cut(s) 454
MlyI GAGTC 1 cut(s) 1047
MmeI TCCRAC 1 cut(s) 804
MnlI CCTC 7 cut(s) 179, 284, 492, 502, 853, 1027, 1245
MroNI GCCGGC 1 cut(s) 911
MroXI GAANNNNTTC 1 cut(s) 1086
MseI TTAA 3 cut(s) 230, 1242, 1389
MslI CAYNNNNRTG 2 cut(s) 869, 940
MspI CCGG 4 cut(s) 912, 1123, 1247, 1338
MspR9I CCNGG 6 cut(s) 40, 111, 358, 896, 1123, 1338
MunI CAATTG 1 cut(s) 410
MvaI CCWGG 4 cut(s) 40, 111, 358, 896
MvnI CGCG 2 cut(s) 456, 545
MwoI GCNNNNNNNGC 6 cut(s) 339, 348, 399, 443, 509, 691
NaeI GCCGGC 1 cut(s) 913
NciI CCSGG 2 cut(s) 1123, 1338
NdeII GATC 4 cut(s) 42, 750, 757, 765
NgoMIV GCCGGC 1 cut(s) 911
NlaIII CATG 6 cut(s) 287, 355, 499, 868, 874, 1444
NlaIV GGNNCC 2 cut(s) 202, 1406
NmeAIII GCCGAG 2 cut(s) 201, 979
NspI RCATGY 1 cut(s) 874
PdiI GCCGGC 1 cut(s) 913
PdmI GAANNNNTTC 1 cut(s) 1086
PfeI GAWTC 5 cut(s) 139, 321, 361, 782, 1037
PflFI GACNNNGTC 1 cut(s) 290
PflMI CCANNNNNTGG 2 cut(s) 134, 1221
PfoI TCCNGGA 2 cut(s) 894, 1336
PkrI GCNGC 2 cut(s) 532, 910
PleI GAGTC 1 cut(s) 1047
PpsI GAGTC 1 cut(s) 1047
Psp6I CCWGG 4 cut(s) 38, 109, 356, 894
PspFI CCCAGC 1 cut(s) 1375
PspGI CCWGG 4 cut(s) 38, 109, 356, 894
PspN4I GGNNCC 2 cut(s) 202, 1406
PspPI GGNCC 3 cut(s) 113, 200, 1197
PsuI RGATCY 1 cut(s) 750
PsyI GACNNNGTC 1 cut(s) 290
RsaI GTAC 2 cut(s) 347, 1425
RsaNI GTAC 2 cut(s) 346, 1424
RseI CAYNNNNRTG 2 cut(s) 869, 940
SaqAI TTAA 3 cut(s) 230, 1242, 1389
SatI GCNGC 2 cut(s) 531, 909
Sau3AI GATC 4 cut(s) 42, 750, 757, 765
Sau96I GGNCC 3 cut(s) 113, 200, 1197
SchI GAGTC 1 cut(s) 1047
ScrFI CCNGG 6 cut(s) 40, 111, 358, 896, 1123, 1338
SduI GDGCHC 3 cut(s) 1098, 1177, 1497
SfaNI GCATC 2 cut(s) 424, 1362
SinI GGWCC 1 cut(s) 113
SmiMI CAYNNNNRTG 2 cut(s) 869, 940
SmlI CTYRAG 2 cut(s) 990, 1176
SmoI CTYRAG 2 cut(s) 990, 1176
Sse9I AATT 7 cut(s) 13, 410, 550, 975, 1146, 1275, 1386
SsiI CCGC 4 cut(s) 543, 668, 995, 1152
SspMI CTAG 1 cut(s) 609
StyD4I CCNGG 6 cut(s) 38, 109, 356, 894, 1121, 1336
StyI CCWWGG 1 cut(s) 703
TaaI ACNGT 4 cut(s) 238, 628, 800, 1105
TaqI TCGA 5 cut(s) 477, 760, 876, 1051, 1299
TasI AATT 7 cut(s) 13, 410, 550, 975, 1146, 1275, 1386
TatI WGTACW 1 cut(s) 345
TfiI GAWTC 5 cut(s) 139, 321, 361, 782, 1037
Tru1I TTAA 3 cut(s) 230, 1242, 1389
Tru9I TTAA 3 cut(s) 230, 1242, 1389
TscAI CASTG 1 cut(s) 135
TseI GCWGC 2 cut(s) 530, 908
TspDTI ATGAA 2 cut(s) 504, 1451
TspGWI ACGGA 2 cut(s) 932, 1299
TspRI CASTG 1 cut(s) 135
Tth111I GACNNNGTC 1 cut(s) 290
Van91I CCANNNNNTGG 2 cut(s) 134, 1221
VneI GTGCAC 1 cut(s) 1094
VpaK11BI GGWCC 1 cut(s) 113
XapI RAATTY 1 cut(s) 975
XceI RCATGY 1 cut(s) 874
XcmI CCANNNNNNNNNTGG 2 cut(s) 820, 1010
XmnI GAANNNNTTC 1 cut(s) 1086
XspI CTAG 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.