Rh4CG414800

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008280
Physical Location & Seq
Forward (+)
65941051 .. 65945925
4875 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG414800.1

Sequence Viewer

Length: 1704 bp
ATGCACCCATACAACCGGCTACCGAGTAGCGGACACAGCAGCCCGTCGCCGCCGCATTCCCCGCTCCGTTCGCCCCGGTTCCGCCAAACCCGGTCCAAAGCCTCGGGCCGGTTCATTAACCAGCCAGGTCGGACCCTAATCCAGCGCATCGCCTGGCACCTCCTCTCCGTCCTCATCCGCCGCCAAGGCTTCTTCCTCTTCGCTCCTCTCCTCTACATCTCCGGCATGCTCCTCTACATGGGCTCCGGCTCCTTCCCCGACGACCGCCTCCCCATCATCCCCCGCCATTTCGCTCCCGGCTCCCGCTACCGGAGCCCCCAGCTCTACGACAAGCTCCGCCCCGAAATGGACGCCGATAACTCCTCCGCCGATGCGATTTCAACTGTCTGGAAGCATTCGTTCAGTGCGTATAAGGGTGTGGAATGGAGACCCTGTGTAAACACCTCCTCTGGAGTTTTACCCGAGTCAAATGGTTATATATATGTTGAGGCAAATGGTGGTCTAAATCAGCAGAGGACTTCTATATGCAATGCGGTTGCCGTTGCAGGATATCTTAATGCAACACTTTTAATTCCCAATTTTCATTTTCATAGCATCTGGAGAGATCCAAGTAAATTTGGTGACATTTATGATGAAGAATATTTCATCAGTTCGTTGGAAAATGATGTGCGAGTGGTCAACAGGATTCCTGAGTATTTGATGGAACGATTTGATCACAATATGACCAATGTTTACAACTTCAGAATTAAAGCATGGTCGCCAATTTCGTATTATAAGGATGCGGTTCTCCCGAGGCTACTTGAAGAAAAGGTTATACGGATTTCTCCTTTTGCCAATCGGTTGTCATTTGATTCTCCTCCTGCTGTTCAACGACTTAGATGCTTGGCTAATTATAAAGCTTTAAGGTTTTCAAGTCCTATATTAACGTTGGGAGAAACTTTAGTTGCAAGAATGAAAGAACGCAGTGCAAATTACGGTGGCAAATATATTTCTGTGCATCTTCGCTTTGAAGAGGACATGGTTGCCTTTTCTTGTTGTGAATTTGATGGTGGAGCGCAAGAAAAGGAAGACATGACAGAAGCAAGGGAAAGGGGTTGGAAAGGGAAATTTAACAAACCTGGAAGGGTTATACGTCCTGGAGCAAACAGGATCAATGGAAAATGTCCCCTCACTCCTTTAGAGGTTGGCTTGATGCTTAGGGGAATGGGATTTGATAAGAACTCATTTATCTACTTGGCATCTGGAAAGATATACGATGCTGAGAGAACAATGGCTCCATTGCTGGAAATGTTTCCGAATTTGATCACAAAACAGATGATTGCTACAGATGAGGAACTTGCTCCATACAAGAATTATTCTTCCAGGATGGCTGCAATAGATTACACTGTTTGTCTTCACAGTGAGGTATTTGTGACAACTCAAGGAGGAAACTTTCCCCATTTTCTGATGGGACACAGAAGATACTTGTATGGTGGACACTCCAAGACTATTAGGCCTGACAAGCGGAAGTTAGCATTACTCTTTGATAATCCAAAAATCGGATGGAAGACTTTCAAGCGGCAATTGCTTAGTATGCGGTCTCATAGTGATTCAAAGGGGTTTGAGCTTAAGAGGCCAAATGATTCTATATATACCTTCCCTTGCCCAGATTGCATGTGCCGAACAAACAAGTCAGAGGATTCAAGACCGTCATTAGCTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0007155 GO:0008150 GO:0012505 GO:0022610 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

567

Amino Acids

64.8

Weight (kDa)

9.54

Isoelectric Point (pI)

48.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 159 - 489 2.9e-102 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 774, 894
AccB1I GGYRCC 1 cut(s) 156
AccBSI CCGCTC 1 cut(s) 64
AclI AACGTT 1 cut(s) 926
AclWI GGATC 2 cut(s) 599, 1157
AcsI RAATTY 4 cut(s) 614, 1040, 1106, 1297
AcuI CTGAAG 1 cut(s) 724
AcyI GRCGYC 1 cut(s) 351
AfiI CCNNNNNNNGG 6 cut(s) 29, 108, 238, 309, 346, 1538
AflII CTTAAG 1 cut(s) 1607
AgsI TTSAA 8 cut(s) 381, 803, 869, 912, 1010, 1555, 1593, 1683
AjnI CCWGG 5 cut(s) 124, 152, 1117, 1135, 1361
AloI GAACNNNNNNTCC 2 cut(s) 1258, 1290
AluBI AGCT 5 cut(s) 322, 334, 899, 1606, 1697
AluI AGCT 5 cut(s) 322, 334, 899, 1606, 1697
Alw26I GTCTC 2 cut(s) 421, 1584
AlwI GGATC 2 cut(s) 599, 1157
Ama87I CYCGRG 3 cut(s) 103, 461, 790
AoxI GGCC 3 cut(s) 106, 1493, 1613
ApeKI GCWGC 2 cut(s) 39, 1370
ApoI RAATTY 4 cut(s) 614, 1040, 1106, 1297
Asp700I GAANNNNTTC 2 cut(s) 1290, 1550
AspLEI GCGC 2 cut(s) 147, 1057
AspS9I GGNCC 3 cut(s) 93, 106, 132
AsuC2I CCSGG 3 cut(s) 76, 91, 297
AsuHPI GGTGA 1 cut(s) 632
AvaI CYCGRG 3 cut(s) 103, 461, 790
AvaII GGWCC 2 cut(s) 93, 132
BanI GGYRCC 1 cut(s) 156
BanII GRGCYC 2 cut(s) 245, 317
BbsI GAAGAC 3 cut(s) 1074, 1385, 1553
BbvI GCAGC 2 cut(s) 51, 1357
BccI CCATC 6 cut(s) 281, 694, 1040, 1360, 1441, 1536
BceAI ACGGC 1 cut(s) 524
BcgI CGANNNNNNTGC 2 cut(s) 861, 895
BciT130I CCWGG 5 cut(s) 126, 154, 1119, 1137, 1363
BclI TGATCA 2 cut(s) 712, 1302
BcnI CCSGG 3 cut(s) 76, 91, 297
BcoDI GTCTC 2 cut(s) 421, 1584
BfmI CTRYAG 1 cut(s) 1323
BfrI CTTAAG 1 cut(s) 1607
BglI GCCNNNNNGGC 1 cut(s) 186
BisI GCNGC 6 cut(s) 40, 50, 53, 181, 1371, 1559
BlsI GCNGC 6 cut(s) 41, 51, 54, 182, 1372, 1560
Bme1390I CCNGG 8 cut(s) 76, 91, 126, 154, 297, 1119, 1137, 1363
Bme18I GGWCC 2 cut(s) 93, 132
BmeT110I CYCGRG 3 cut(s) 103, 461, 790
BmgT120I GGNCC 3 cut(s) 93, 106, 132
BmiI GGNNCC 8 cut(s) 80, 134, 158, 244, 250, 301, 314, 1275
BmrFI CCNGG 8 cut(s) 76, 91, 126, 154, 297, 1119, 1137, 1363
BmsI GCATC 9 cut(s) 156, 361, 603, 769, 869, 1006, 1182, 1246, 1247
BpiI GAAGAC 3 cut(s) 1074, 1385, 1553
BpmI CTGGAG 3 cut(s) 471, 619, 1158
Bpu10I CCTNAGC 1 cut(s) 1196
BpuEI CTTGAG 1 cut(s) 1404
BpuMI CCSGG 3 cut(s) 76, 91, 297
BsaAI YACGTR 1 cut(s) 1701
BsaHI GRCGYC 1 cut(s) 351
BsaI GGTCTC 2 cut(s) 421, 1584
BsaJI CCNNGG 4 cut(s) 74, 102, 184, 791
BsaWI WCCGGW 1 cut(s) 309
BsaXI ACNNNNNCTCC 8 cut(s) 149, 179, 227, 257, 418, 448, 1258, 1288
Bsc4I CCNNNNNNNGG 6 cut(s) 29, 108, 238, 309, 346, 1538
Bse118I RCCGGY 2 cut(s) 15, 108
Bse3DI GCAATG 2 cut(s) 535, 1277
BseBI CCWGG 5 cut(s) 126, 154, 1119, 1137, 1363
BseDI CCNNGG 4 cut(s) 74, 102, 184, 791
BseGI GGATG 5 cut(s) 174, 276, 784, 1371, 1547
BseLI CCNNNNNNNGG 6 cut(s) 29, 108, 238, 309, 346, 1538
BseMI GCAATG 2 cut(s) 535, 1277
BseMII CTCAG 2 cut(s) 681, 1251
BseRI GAGGAG 7 cut(s) 152, 195, 200, 221, 352, 436, 846
BseXI GCAGC 2 cut(s) 51, 1357
BseYI CCCAGC 1 cut(s) 318
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 3 cut(s) 108, 1495, 1615
BshNI GGYRCC 1 cut(s) 156
BsiEI CGRYCG 1 cut(s) 265
BsiHKCI CYCGRG 3 cut(s) 103, 461, 790
BsiSI CCGG 8 cut(s) 16, 76, 91, 109, 222, 246, 297, 310
BslFI GGGAC 2 cut(s) 1149, 1464
BslI CCNNNNNNNGG 6 cut(s) 29, 108, 238, 309, 346, 1538
BsmAI GTCTC 2 cut(s) 421, 1584
BsmFI GGGAC 2 cut(s) 1149, 1464
BsmI GAATGC 2 cut(s) 55, 394
BsnI GGCC 3 cut(s) 108, 1495, 1615
Bso31I GGTCTC 2 cut(s) 421, 1584
BsoBI CYCGRG 3 cut(s) 103, 461, 790
Bsp1286I GDGCHC 2 cut(s) 245, 317
Bsp143I GATC 4 cut(s) 604, 712, 1149, 1302
BspANI GGCC 3 cut(s) 108, 1495, 1615
BspCNI CTCAG 2 cut(s) 682, 1252
BspLI GGNNCC 8 cut(s) 80, 134, 158, 244, 250, 301, 314, 1275
BspPI GGATC 2 cut(s) 599, 1157
BspT107I GGYRCC 1 cut(s) 156
BspTI CTTAAG 1 cut(s) 1607
BspTNI GGTCTC 2 cut(s) 421, 1584
BsrBI CCGCTC 1 cut(s) 64
BsrDI GCAATG 2 cut(s) 535, 1277
BsrFI RCCGGY 2 cut(s) 15, 108
BssAI RCCGGY 2 cut(s) 15, 108
BssECI CCNNGG 4 cut(s) 74, 102, 184, 791
BssMI GATC 4 cut(s) 604, 712, 1149, 1302
BssNI GRCGYC 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 184
Bst2UI CCWGG 5 cut(s) 126, 154, 1119, 1137, 1363
Bst4CI ACNGT 5 cut(s) 385, 977, 1387, 1400, 1689
Bst6I CTCTTC 2 cut(s) 203, 1005
BstACI GRCGYC 1 cut(s) 351
BstAFI CTTAAG 1 cut(s) 1607
BstBAI YACGTR 1 cut(s) 1701
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 5 cut(s) 690, 875, 1196, 1260, 1568
BstF5I GGATG 5 cut(s) 174, 276, 784, 1371, 1547
BstHHI GCGC 2 cut(s) 147, 1057
BstKTI GATC 4 cut(s) 607, 715, 1152, 1305
BstMAI GTCTC 2 cut(s) 421, 1584
BstMBI GATC 4 cut(s) 604, 712, 1149, 1302
BstMCI CGRYCG 1 cut(s) 265
BstNI CCWGG 5 cut(s) 126, 154, 1119, 1137, 1363
BstNSI RCATGY 2 cut(s) 229, 1657
BstSCI CCNGG 8 cut(s) 74, 89, 124, 152, 295, 1117, 1135, 1361
BstSFI CTRYAG 1 cut(s) 1323
BstV1I GCAGC 2 cut(s) 51, 1357
BstV2I GAAGAC 3 cut(s) 1074, 1385, 1553
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
BsuRI GGCC 3 cut(s) 108, 1495, 1615
BtgZI GCGATG 1 cut(s) 133
BtsCI GGATG 5 cut(s) 174, 276, 784, 1371, 1547
BtsI GCAGTG 1 cut(s) 970
BtsIMutI CAGTG 4 cut(s) 409, 970, 1383, 1405
Cac8I GCNNGC 1 cut(s) 227
CfoI GCGC 2 cut(s) 147, 1057
Cfr10I RCCGGY 2 cut(s) 15, 108
Cfr13I GGNCC 3 cut(s) 93, 106, 132
CseI GACGC 1 cut(s) 359
CviAII CATG 6 cut(s) 226, 238, 753, 1018, 1072, 1654
DdeI CTNAG 5 cut(s) 690, 875, 1196, 1260, 1568
DpnI GATC 4 cut(s) 606, 714, 1151, 1304
DpnII GATC 4 cut(s) 604, 712, 1149, 1302
Eam1104I CTCTTC 2 cut(s) 203, 1005
EarI CTCTTC 2 cut(s) 203, 1005
EciI GGCGGA 4 cut(s) 71, 167, 326, 355
Eco130I CCWWGG 1 cut(s) 184
Eco147I AGGCCT 1 cut(s) 1495
Eco24I GRGCYC 2 cut(s) 245, 317
Eco31I GGTCTC 2 cut(s) 421, 1584
Eco32I GATATC 1 cut(s) 551
Eco47I GGWCC 2 cut(s) 93, 132
Eco57I CTGAAG 1 cut(s) 724
Eco88I CYCGRG 3 cut(s) 103, 461, 790
EcoRII CCWGG 5 cut(s) 124, 152, 1117, 1135, 1361
EcoRV GATATC 1 cut(s) 551
EcoT14I CCWWGG 1 cut(s) 184
EcoT38I GRGCYC 2 cut(s) 245, 317
ErhI CCWWGG 1 cut(s) 184
FaeI CATG 6 cut(s) 229, 241, 756, 1021, 1075, 1657
FaqI GGGAC 2 cut(s) 1149, 1464
FatI CATG 6 cut(s) 225, 237, 752, 1017, 1071, 1653
FauI CCCGC 3 cut(s) 69, 290, 311
FbaI TGATCA 2 cut(s) 712, 1302
Fnu4HI GCNGC 6 cut(s) 40, 50, 53, 181, 1371, 1559
FokI GGATG 5 cut(s) 161, 263, 791, 1378, 1554
FriOI GRGCYC 2 cut(s) 245, 317
Fsp4HI GCNGC 6 cut(s) 40, 50, 53, 181, 1371, 1559
GlaI GCGC 2 cut(s) 146, 1056
GluI GCNGC 6 cut(s) 40, 50, 53, 181, 1371, 1559
GsaI CCCAGC 1 cut(s) 322
GsuI CTGGAG 3 cut(s) 471, 619, 1158
HaeIII GGCC 3 cut(s) 108, 1495, 1615
HapII CCGG 8 cut(s) 16, 76, 91, 109, 222, 246, 297, 310
HgaI GACGC 1 cut(s) 359
HhaI GCGC 2 cut(s) 147, 1057
Hin1I GRCGYC 1 cut(s) 351
Hin1II CATG 6 cut(s) 229, 241, 756, 1021, 1075, 1657
Hin6I GCGC 2 cut(s) 145, 1055
HinP1I GCGC 2 cut(s) 145, 1055
HincII GTYRAC 1 cut(s) 679
HindII GTYRAC 1 cut(s) 679
HindIII AAGCTT 1 cut(s) 897
HinfI GANTC 6 cut(s) 464, 685, 851, 1589, 1622, 1679
HpaII CCGG 8 cut(s) 16, 76, 91, 109, 222, 246, 297, 310
HphI GGTGA 1 cut(s) 632
Hpy166II GTNNAC 4 cut(s) 439, 679, 733, 1475
Hpy188I TCNGA 6 cut(s) 132, 743, 1296, 1446, 1541, 1675
Hpy188III TCNNGA 7 cut(s) 388, 450, 598, 689, 790, 1242, 1683
Hpy8I GTNNAC 4 cut(s) 439, 679, 733, 1475
Hpy99I CGWCG 2 cut(s) 49, 263
HpyAV CCTTC 3 cut(s) 262, 1116, 1645
HpyCH4III ACNGT 5 cut(s) 385, 977, 1387, 1400, 1689
HpyCH4IV ACGT 3 cut(s) 926, 1132, 1700
HpyCH4V TGCA 9 cut(s) 4, 528, 545, 560, 947, 968, 997, 1373, 1653
HpyF3I CTNAG 5 cut(s) 690, 875, 1196, 1260, 1568
HpySE526I ACGT 3 cut(s) 926, 1132, 1700
Hsp92I GRCGYC 1 cut(s) 351
Hsp92II CATG 6 cut(s) 229, 241, 756, 1021, 1075, 1657
HspAI GCGC 2 cut(s) 145, 1055
Ksp22I TGATCA 2 cut(s) 712, 1302
Kzo9I GATC 4 cut(s) 604, 712, 1149, 1302
Lsp1109I GCAGC 2 cut(s) 51, 1357
LweI GCATC 9 cut(s) 156, 361, 603, 769, 869, 1006, 1182, 1246, 1247
MaeII ACGT 3 cut(s) 926, 1132, 1700
MaeIII GTNAC 2 cut(s) 620, 1411
MalI GATC 4 cut(s) 606, 714, 1151, 1304
MbiI CCGCTC 1 cut(s) 64
MboI GATC 4 cut(s) 604, 712, 1149, 1302
MfeI CAATTG 1 cut(s) 1562
MflI RGATCY 1 cut(s) 604
MhlI GDGCHC 2 cut(s) 245, 317
MlyI GAGTC 1 cut(s) 473
MmeI TCCRAC 3 cut(s) 110, 636, 1076
MroXI GAANNNNTTC 2 cut(s) 1290, 1550
MseI TTAA 8 cut(s) 117, 555, 569, 747, 902, 923, 1110, 1608
MspCI CTTAAG 1 cut(s) 1607
MspI CCGG 8 cut(s) 16, 76, 91, 109, 222, 246, 297, 310
MspR9I CCNGG 8 cut(s) 76, 91, 126, 154, 297, 1119, 1137, 1363
MunI CAATTG 1 cut(s) 1562
Mva1269I GAATGC 2 cut(s) 55, 394
MvaI CCWGG 5 cut(s) 126, 154, 1119, 1137, 1363
NciI CCSGG 3 cut(s) 76, 91, 297
NdeII GATC 4 cut(s) 604, 712, 1149, 1302
NlaIII CATG 6 cut(s) 229, 241, 756, 1021, 1075, 1657
NlaIV GGNNCC 8 cut(s) 80, 134, 158, 244, 250, 301, 314, 1275
NmuCI GTSAC 2 cut(s) 620, 1411
NspI RCATGY 2 cut(s) 229, 1657
PaeI GCATGC 1 cut(s) 229
PceI AGGCCT 1 cut(s) 1495
PcsI WCGNNNNNNNCGW 2 cut(s) 404, 764
PctI GAATGC 2 cut(s) 55, 394
PdmI GAANNNNTTC 2 cut(s) 1290, 1550
PfeI GAWTC 5 cut(s) 685, 851, 1589, 1622, 1679
PfoI TCCNGGA 2 cut(s) 1135, 1361
PkrI GCNGC 6 cut(s) 41, 51, 54, 182, 1372, 1560
PleI GAGTC 1 cut(s) 472
PpsI GAGTC 1 cut(s) 472
Ppu21I YACGTR 1 cut(s) 1701
PsiI TTATAA 2 cut(s) 774, 894
Psp1406I AACGTT 1 cut(s) 926
Psp6I CCWGG 5 cut(s) 124, 152, 1117, 1135, 1361
PspFI CCCAGC 1 cut(s) 318
PspGI CCWGG 5 cut(s) 124, 152, 1117, 1135, 1361
PspN4I GGNNCC 8 cut(s) 80, 134, 158, 244, 250, 301, 314, 1275
PspPI GGNCC 3 cut(s) 93, 106, 132
PsuI RGATCY 1 cut(s) 604
SaqAI TTAA 8 cut(s) 117, 555, 569, 747, 902, 923, 1110, 1608
SatI GCNGC 6 cut(s) 40, 50, 53, 181, 1371, 1559
Sau3AI GATC 4 cut(s) 604, 712, 1149, 1302
Sau96I GGNCC 3 cut(s) 93, 106, 132
SchI GAGTC 1 cut(s) 473
ScrFI CCNGG 8 cut(s) 76, 91, 126, 154, 297, 1119, 1137, 1363
SduI GDGCHC 2 cut(s) 245, 317
SfaNI GCATC 9 cut(s) 156, 361, 603, 769, 869, 1006, 1182, 1246, 1247
SfcI CTRYAG 1 cut(s) 1323
SinI GGWCC 2 cut(s) 93, 132
SmlI CTYRAG 2 cut(s) 1419, 1607
SmoI CTYRAG 2 cut(s) 1419, 1607
SphI GCATGC 1 cut(s) 229
SseBI AGGCCT 1 cut(s) 1495
SspI AATATT 1 cut(s) 641
StuI AGGCCT 1 cut(s) 1495
StyD4I CCNGG 8 cut(s) 74, 89, 124, 152, 295, 1117, 1135, 1361
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 5 cut(s) 385, 977, 1387, 1400, 1689
TaiI ACGT 3 cut(s) 929, 1135, 1703
TauI GCSGC 4 cut(s) 52, 55, 183, 1561
TfiI GAWTC 5 cut(s) 685, 851, 1589, 1622, 1679
Tru1I TTAA 8 cut(s) 117, 555, 569, 747, 902, 923, 1110, 1608
Tru9I TTAA 8 cut(s) 117, 555, 569, 747, 902, 923, 1110, 1608
TscAI CASTG 4 cut(s) 409, 970, 1390, 1405
TseFI GTSAC 2 cut(s) 620, 1411
TseI GCWGC 2 cut(s) 39, 1370
Tsp45I GTSAC 2 cut(s) 620, 1411
TspDTI ATGAA 6 cut(s) 103, 572, 578, 634, 648, 968
TspGWI ACGGA 3 cut(s) 56, 157, 832
TspRI CASTG 4 cut(s) 409, 970, 1390, 1405
Vha464I CTTAAG 1 cut(s) 1607
VpaK11BI GGWCC 2 cut(s) 93, 132
XapI RAATTY 4 cut(s) 614, 1040, 1106, 1297
XceI RCATGY 2 cut(s) 229, 1657
XcmI CCANNNNNNNNNTGG 1 cut(s) 1539
XmnI GAANNNNTTC 2 cut(s) 1290, 1550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.