Rorug04G0426600

Secretory carrier-associated membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0021420
Physical Location & Seq
Reverse (-)
58542818 .. 58550117
7300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0426600.1

Sequence Viewer

Length: 5220 bp
ATGGCAATCGAAGGTGGGAAGAAATCTAGGAGAGATCGTCTCAAAGCGATTGAGGAGAAGGCTCAAAAGTTGTGGGAAGAAAATGGTGTTTTCAGGGCTGAATCTTGTGAAAAGCCCCCTGAACCAGGGGAGAAGTTCTTTGGGAACTTCCCTCTTCCTTACATGAATGGTTTTTTGCATCTAGGGCATGCATTCTCACTCTCGAAGCTAGAGTTTTCTGCTCGTTTTCATAGGTTAAGAGGTGCTCATGTGCTACTCCCGGTTGGTTTCCACCTCACTGGCATGCCCATCAAAAGTATTGGCCTGTCTGCCATTGATATATCAGAACTCCAGAATCCATCGAACTGGTTGAGCTACTTCCCTCGAGTTGCAGTGAAAGAGCTCAAGGCATTTGGCTTGGGCTGTGACTGGAGGCGCTCCTTTATTACCACAGACATGAACCCATACTTTGACAAGTTTCTGAGATGGCAGATGAGGAAACTAAAATCTATGGGTAAGATTGTGAAGGATATCCGTTACACAATCTACTCTCCTTTGGATGGCCAGCCATGTGCAGATCATGATAGGGCAACTGGTGAAGGAGTTCGGCCCCAAGAGTACACCATCATCAAAATGGAGGTGGTGGCACCTTTTCCTCCTAAACTGGGAGTGTTGAAGGGAAAGAAAGTCTTCCTTGCTGCCGCAACATTGAGACCTGAGACTTCTTATGGTCAAACAAATGCATGGGTGTTGCCTGATGGCAAGTATGGAGCCTTTGAAATCAATGAAACAGATGTCTTCATTCTTACGCAGAGAGCAGCACTTAATCTTGCCTATCAGAAGTACTCTAGGGTTCCAGAAAAGCCTTCTTGCTTGGTTGAGATGACTGGCTATGATTTGATTGGCCTTCCATTGAAGTCTCCACTTGCACTCAATCAGATCGTTTATGCCCTTCCTATGTTGACCGTCCTAACAGACAAAGGTACTGGGATATTGACTAGTGTACCTAGTGATGCTCCTGATGATTATATGGCCTTGGAAGATTTAAAATTGAAACCAGCGTTTAGAGAAAAATATGGTGTGAAGGATGAATGGGTCATGCCCTTTGATATCATTCCAATAATCAAAATTCCGGAATATGGAGACAAGGCTGCTAAAAAGGTTGTGGAAGATCTCAAAATCAAAAGTCAGAATGAGATAGAGAAGCTAGCAGAAGCCAAGAGGTTGACATCCTTGAAAGGTTTAACTGAAGGAAAACTGCTTGTGGGAGAATTCAAAGAAAGGATAGTCCAGGAGGCAAAACCATTGATAAGGAGCAGGCTATTAGAGGCAGGTGATGCAATCATGTATAGCGAGCCTGAAAAGCAGGTAGTTTCACGATCTGGTGATGAATGTGTTGTTGCTCTTATAGATCAATGGTGCATCACATATGGGGAAGCAGAATGGAAAAAAACTGCTCAGGAGTGCTTGTCATGCATAAATTTATATTCTGACACGACACGACATGGATTTGTACACACATTGAATTGGTTGAGTCAGTGGGCTTGTTCTCGATCTTTTGGGCTTGGTACACGCATTCCCTGGGATGAAGAATACTTGGTCGAGCCGTTATCCGATTCAACTCTTTACATGGCTTATTACACCATTGCTCACCTGTTACACAATGAAGACATGTATGGCAGCTCCAACAGCACATCTGCAGTAAGAGCTGAACAAATGACCGATGAGGTTTGGGATTTTATTTTCTGTGATGGCTCGTATCCACAATCATCTGATATCACACCATCAATTCTTAACAAGATGAAGCAGGAGTTTGAATATTGGTATCCTTTTGATCTTGAGGTGTCTGGTAAGGATCTAATCCAGAATCATACCTTCTGCATTTACAACCATACTGCACTTTTGCCCAAGAAGCACTGGCCTAGTGCGTTTAGATGCAATGGGCACCTGATGCTCAAATCCACGAAGAATGCTAATGGTGCTGGAAATTTTATATCATTGAGACACGCAATTGATCACTATTCTGCTGATGCCACGCGGTTTGCTTTGGCTGATATTAGTGATGGTATTGACGATGCATACTTTTCATTTGAAACTGCAAATGCGGCCATCGTACGTCTCACTAAGGAGATAGATTGGATTGATCGGATGAAAGATAAAGATTTGGGTAAAGACTCTTCTCTGAGAATAGACTCCCCTTGTACCTTCTCTGACCGGGTCTTTCTGAATGAAATAAATATTGCTGTGAAGAGGACTGAGCAAAATTACCAGGGTTGCATGTTCAGGGAAGCCCTAAAGACTGGCTTTTACGATCTTCAAGCTGCCAGGGATTGGTACAGGATTTCATGTGGTGGTACTAATGCAATGAACCACGATTTGGTGTGGCGTTTTATAGATGTGCAGACCCGTCTTATTGCTCCAATTTGTCCCCACTATGCAGAACATGTCTGGAGGGAACTTTTAGAGAGGGAAGGATTTGCAGTAAATGCAGGCTGGCCCGCGGCTGATGCTCCAGATCTAACACTCCAGAGTGCGAATAAGTATTTGCAAGACTCAATTGATTCAATAAGGAAGCTGCATAATAAACAACTACCAACTTCAAAGAAAACCAATATGGGTGCTTCAGCTACTTCTTTGACAGAAGCGTTGATATATGTTAAGGAGAAATATGACGGATGGAAAGCAGAATGCTTAAGAATACTCCAACATGACTTAAATAGAGATACTCTTACTTCTGCTCACGAGGACAAGGAGATATTTGAGGCATTGAAGGCTAGTTCTTTTGGTCAAGATGATGATTTTAGCCAAAAGAATTGTATGGAATTTTTGAAGTTCAAGAAGGAGAAGGCAGTTGCACTTGGGATTCAGGCATTGGACTTGAAGCTACCTTTCGGAGAGATTGAAGTCCTTGAACAGAACTTGGACTTGATTAAAAAACAAATACATGGTCTTGAAGAGGTACAAGTTTTGTCAGCTATCAATGCTGCTGATATTGCTAGAGCTGGTCCTCATGCTAGACTGCTAGAGAAGAATCCTCCCTCTCCTGGAAGCCCAACTACGGTATCCAAAGGTGATAACATTCTCCTAGAGATTAAGATTCTCCTAGAGATTGAAGCAGAGGTTCGAGAATGGTGGGAAGAGAAAGGCGTTTTTGTCAATGCTGATCAATCTCCTGACAACCCTCACTATCCTAACGATGAACTGGGGGATGATCGGAAGAAGTGGTTTGAAAACTATCTGTTTGGTGGTTTATCGCGACGGGCATTCCTGATATCTAAGCTAGAGTGTTGTGCAGCTTATCATAGATTAAGAGATGACAATGTGCCATGGCAAATCAAAGCCTCAGGCGCAGAGGAACTTCCGGAGAAGATTATAAATCTGTTGGTTGGAGAACCACCAGTATTTCCAACAGTTTGGAGAGCTGCCTATTCTACTGATGCCATGAGACTCTCCTTAGCTTGTTCTTGTGATGGTGATGATTCTCCGAGAATTTTATTTAATACTGCAAATTCTGCTATCCGAAAGCTCACTAAAGAAATGTCATGGATTAGTGAACAACTAGCTTATATGGCTGCTGCTGCAGACTCTTCTTCTTTTGTGAGAAGAATCAGAAGAGATGGCCCCCCCAGTACTTTTGCGGACAAGGTGTTTGCGAATGAGATCAATATTGCTGTGCATAACAGTGATAACTATTACCGGGCTTGCATGTTTCAAGAAGCCCTCATTTCTGGCTTTCTTCAACTTCAGGCTGCCAGGGACTCGTATAGAATTTCATGTGGTCCTCATGGCATGAACCATGATTTGGTGCTGCGCTATATTGATGTGCATACACGTCTAATTGCTCCAATCTGTCCACACTATGCTGAATATGTCTGGCGGGAATATTTAAAAAAGGAGGGGTTTGTGGTAATTAAGTCAGGCTGGCCTGCCGCTGATGCCCCGGATTTGATACTCCAAAGTGCCAATAAGTATTTGCAAGATCTAATTGAACTTATGAGGGAGCTTGAAAAGAAGTTTCGAGCTTTAAATGATGGTAATTGTGATGTGACAGAAGAAGAAACGAAGGCAGTAGGTTTGATATATGTCAAGGAGGAAATTGATGGATGGGAGGGTAAGTGCTTGAGGATACTTCAAGATAACTTTGACAGCGAGACAAACACCTTTTTTGCTCAGGACGAGGACATGCTAGACGCATTAATATCGTACATATATGGGGATTTTACCATTCGTCAGGATATAGATTATAGGCAAACTGAACAGCTCTGTATGTCTTTTCTGAAGTTGAAGAAGGATGAAGCACTTAATTTTGGGGCTGATGCCTTAGAGTTGAAGCTACCATTTGGAGAGATTGAGGTCGTTCAAGAAAACTTGGACTTGATTAAGAGAGAAATTGGCCTTCATGAGGTTAAGGTACAAGTTTTCTCTGGTACCACCGGTCTTGAAGATTTTACACTGGATAGTCCTCCATCCCCTGGAAGGCCAGCTGCTGCCTTGGTGAGCCGTGACTTCTATGAGAAAATGGAACGTTCTGCTCCGGGAGTATGGGAATTGAAACGTCGTCTTCAGTTATCAGATAAAGTTCTTACTCCCTCGTCTCGTCCTCCCTCTGAGCTATTGTACCAGAAGAAAATGGAACATCCGTTTAAGTTGTTATTCCAGGAGAAAACGGAACATTCTGACCTTGGAGAACAGGAACCGAATGATGAACAATCCCCAGATAATATTCTTGCTCCATTATATCATCCTCCCTATGAGCTATTGTTTCAGGGCTCTTTCAAAAAGGCCAAACGTACTGCTTCTGCCCAGGACAAATGGCTCCTCGTGAACTTGCAATCGAGTAAAGAATTCAGCTCACATCTGATTAATCAAGACACCTGGGCTAATGAAGCTGTCTCGAAAATCATCAGCACTAATTTTATCTTCTGGCAGGCGTATGATGATTCAACTGAGGTCAAGAAGATTTGCAGATTCTACAACTTGGTATATACACCTGTAGTGCTTATCATTGACCCCATAAATGGTGAGAATATGCGTTCGTGGTATGGAATGGTTCAACCAGAACGTCTGCTGGAGGATCTGTTAGTACCATTTTTGGAAAATAGCCCCAAGGATCACCATCATCCAGGAGAAGAAAGTTCTCAGCCTCAGCCAGAGGAAAGTGAAGATGATTCGGTTGGTGACAAAACTGATGAAGAAGATGAGGAATTGCAGTGGGCATTGGAAGCTTCCATGAAAGCATGA

Protein Analysis

1739

Amino Acids

197.92

Weight (kDa)

5.32

Isoelectric Point (pI)

49.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 22 - 686 6.9e-20 tRNA synthetases class I (I, L, M and V)
Anticodon_1 PF08264 731 - 855 1.4e-16 Anticodon-binding domain of tRNA ligase
Anticodon_1 PF08264 1207 - 1335 1.8e-10 Anticodon-binding domain of tRNA ligase
Thioredoxin_7 PF13899 1584 - 1664 9e-13 Thioredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0021420)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0007161
rosa_multiflora Rmu_sc0003826.1_g000002
rosa_rugosa Rorug04G0426600
rosa_samantha Rh5AG054400 Rh5CG062200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3353
AarI CACCTGC 1 cut(s) 1301
Acc36I ACCTGC 2 cut(s) 1301, 1336
Acc65I GGTACC 1 cut(s) 4406
AccB1I GGYRCC 3 cut(s) 625, 1923, 4406
AccB7I CCANNNNNTGG 4 cut(s) 2310, 2356, 3781, 4451
AccII CGCG 3 cut(s) 2017, 2480, 3235
AccIII TCCGGA 2 cut(s) 1111, 3340
AciI CCGC 8 cut(s) 681, 2017, 2084, 2478, 2480, 3617, 3856, 3909
AclI AACGTT 1 cut(s) 4504
AclWI GGATC 3 cut(s) 1842, 5061, 5097
AcoI YGGCCR 2 cut(s) 541, 2085
AcsI RAATTY 9 cut(s) 1107, 1250, 1459, 1966, 2801, 3468, 3487, 3747, 4823
AcuI CTGAAG 5 cut(s) 1248, 2586, 3707, 4277, 4526
AflII CTTAAG 1 cut(s) 2671
AflIII ACRYGT 3 cut(s) 1650, 2422, 3809
AgeI ACCGGT 1 cut(s) 4412
AhlI ACTAGT 1 cut(s) 977
AjiI CACGTC 1 cut(s) 3812
AjuI GAANNNNNNNTTGG 4 cut(s) 2339, 2371, 3764, 3796
Alw21I GWGCWC 2 cut(s) 247, 384
AlwI GGATC 3 cut(s) 1842, 5061, 5097
AlwNI CAGNNNCTG 1 cut(s) 4466
Ama87I CYCGRG 1 cut(s) 363
Aor13HI TCCGGA 2 cut(s) 1111, 3340
ApoI RAATTY 9 cut(s) 1107, 1250, 1459, 1966, 2801, 3468, 3487, 3747, 4823
ArsI GACNNNNNNTTYG 4 cut(s) 2911, 2943, 3611, 3643
AseI ATTAAT 2 cut(s) 4175, 4842
AsiGI ACCGGT 1 cut(s) 4412
Asp700I GAANNNNTTC 2 cut(s) 582, 668
Asp718I GGTACC 1 cut(s) 4406
AspLEI GCGC 3 cut(s) 417, 3329, 3792
AspS9I GGNCC 5 cut(s) 588, 2475, 2984, 3599, 3758
AsuC2I CCSGG 5 cut(s) 260, 2195, 3677, 3920, 4515
AsuNHI GCTAGC 1 cut(s) 1186
AvaI CYCGRG 1 cut(s) 363
AvaII GGWCC 2 cut(s) 2984, 3758
AxyI CCTNAGG 1 cut(s) 3322
BaeGI GKGCMC 1 cut(s) 1926
BaeI ACNNNNGTAYC 4 cut(s) 966, 999, 3024, 3057
BalI TGGCCA 1 cut(s) 543
BanI GGYRCC 3 cut(s) 625, 1923, 4406
BanII GRGCYC 2 cut(s) 384, 4751
BarI GAAGNNNNNNTAC 4 cut(s) 2140, 2172, 3019, 3051
BauI CACGAG 2 cut(s) 2720, 4799
BbsI GAAGAC 4 cut(s) 661, 769, 1653, 4532
Bbv12I GWGCWC 2 cut(s) 247, 384
BbvCI CCTCAGC 1 cut(s) 5124
BceAI ACGGC 2 cut(s) 1570, 4464
BcgI CGANNNNNNTGC 2 cut(s) 4161, 4195
BciVI GTATCC 4 cut(s) 1749, 1815, 3052, 4098
BclI TGATCA 2 cut(s) 1993, 3142
BcnI CCSGG 5 cut(s) 260, 2195, 3677, 3920, 4515
BcuI ACTAGT 1 cut(s) 977
BfmI CTRYAG 3 cut(s) 1677, 3558, 4971
BfoI RGCGCY 1 cut(s) 418
BfrI CTTAAG 1 cut(s) 2671
BfuAI ACCTGC 2 cut(s) 1301, 1336
BfuI GTATCC 4 cut(s) 1749, 1815, 3052, 4098
BglII AGATCT 3 cut(s) 1150, 2494, 3958
BmcAI AGTACT 2 cut(s) 824, 3610
Bme18I GGWCC 2 cut(s) 2984, 3758
BmeT110I CYCGRG 1 cut(s) 363
BmgBI CACGTC 1 cut(s) 3812
BmgT120I GGNCC 5 cut(s) 588, 2475, 2984, 3599, 3758
BmiI GGNNCC 9 cut(s) 590, 627, 751, 834, 1925, 3601, 4408, 4674, 4796
BmrI ACTGGG 4 cut(s) 653, 975, 3191, 3600
BmtI GCTAGC 1 cut(s) 1190
BmuI ACTGGG 4 cut(s) 653, 975, 3191, 3600
BpiI GAAGAC 4 cut(s) 661, 769, 1653, 4532
BplI GAGNNNNNCTC 2 cut(s) 24, 56
BpmI CTGGAG 6 cut(s) 314, 430, 2449, 2475, 2489, 5069
Bpu10I CCTNAGC 4 cut(s) 1437, 3433, 4149, 5124
BpuEI CTTGAG 3 cut(s) 368, 1838, 4120
BpuMI CCSGG 5 cut(s) 260, 2195, 3677, 3920, 4515
BsaBI GATNNNNATC 1 cut(s) 5094
BsaI GGTCTC 1 cut(s) 685
BsaWI WCCGGW 3 cut(s) 1111, 3340, 4412
BsaXI ACNNNNNCTCC 8 cut(s) 22, 52, 1433, 1463, 4079, 4109, 5042, 5072
Bse118I RCCGGY 1 cut(s) 4412
Bse21I CCTNAGG 1 cut(s) 3322
Bse3DI GCAATG 3 cut(s) 1623, 1924, 2349
Bse8I GATNNNNATC 1 cut(s) 5094
BseAI TCCGGA 2 cut(s) 1111, 3340
BseJI GATNNNNATC 1 cut(s) 5094
BseMI GCAATG 3 cut(s) 1623, 1924, 2349
BseRI GAGGAG 2 cut(s) 68, 4787
BseSI GKGCMC 1 cut(s) 1926
BsgI GTGCAG 4 cut(s) 573, 1860, 2399, 3291
Bsh1236I CGCG 3 cut(s) 2017, 2480, 3235
BshNI GGYRCC 3 cut(s) 625, 1923, 4406
BshTI ACCGGT 1 cut(s) 4412
BsiHKAI GWGCWC 2 cut(s) 247, 384
BsiHKCI CYCGRG 1 cut(s) 363
BsiSI CCGG 8 cut(s) 260, 1112, 2194, 3341, 3676, 3920, 4413, 4514
BsiWI CGTACG 1 cut(s) 2092
BslFI GGGAC 2 cut(s) 2391, 3749
BsmBI CGTCTC 3 cut(s) 44, 2102, 4578
BsmFI GGGAC 2 cut(s) 2391, 3749
BsmI GAATGC 5 cut(s) 191, 1554, 1954, 2672, 3242
Bso31I GGTCTC 1 cut(s) 685
BsoBI CYCGRG 1 cut(s) 363
Bsp1286I GDGCHC 4 cut(s) 247, 384, 1926, 4751
Bsp13I TCCGGA 2 cut(s) 1111, 3340
Bsp1407I TGTACA 1 cut(s) 1492
Bsp19I CCATGG 1 cut(s) 3305
Bsp68I TCGCGA 1 cut(s) 3235
BspACI CCGC 8 cut(s) 681, 2017, 2084, 2478, 2480, 3617, 3856, 3909
BspEI TCCGGA 2 cut(s) 1111, 3340
BspFNI CGCG 3 cut(s) 2017, 2480, 3235
BspHI TCATGA 2 cut(s) 559, 4378
BspLI GGNNCC 9 cut(s) 590, 627, 751, 834, 1925, 3601, 4408, 4674, 4796
BspMAI CTGCAG 2 cut(s) 1681, 3562
BspMI ACCTGC 2 cut(s) 1301, 1336
BspOI GCTAGC 1 cut(s) 1190
BspPI GGATC 3 cut(s) 1842, 5061, 5097
BspT107I GGYRCC 3 cut(s) 625, 1923, 4406
BspTI CTTAAG 1 cut(s) 2671
BspTNI GGTCTC 1 cut(s) 685
BsrDI GCAATG 3 cut(s) 1623, 1924, 2349
BsrFI RCCGGY 1 cut(s) 4412
BsrGI TGTACA 1 cut(s) 1492
BssAI RCCGGY 1 cut(s) 4412
BssSI CACGAG 2 cut(s) 2720, 4799
BssT1I CCWWGG 5 cut(s) 1014, 3305, 4470, 4660, 5085
Bst2BI CACGAG 2 cut(s) 2720, 4799
Bst4CI ACNGT 4 cut(s) 946, 3040, 3391, 3662
Bst6I CTCTTC 7 cut(s) 159, 2161, 2222, 2928, 3111, 3571, 3586
BstAFI CTTAAG 1 cut(s) 2671
BstAPI GCANNNNNTGC 4 cut(s) 1316, 1930, 2465, 3491
BstAUI TGTACA 1 cut(s) 1492
BstDSI CCRYGG 2 cut(s) 2478, 3305
BstENI CCTNNNNNAGG 2 cut(s) 123, 4379
BstFNI CGCG 3 cut(s) 2017, 2480, 3235
BstH2I RGCGCY 1 cut(s) 418
BstHHI GCGC 3 cut(s) 417, 3329, 3792
BstNSI RCATGY 7 cut(s) 191, 286, 1654, 2260, 2426, 3688, 4165
BstSFI CTRYAG 3 cut(s) 1677, 3558, 4971
BstSLI GKGCMC 1 cut(s) 1926
BstUI CGCG 3 cut(s) 2017, 2480, 3235
BstV2I GAAGAC 4 cut(s) 661, 769, 1653, 4532
BstX2I RGATCY 5 cut(s) 1150, 1834, 2494, 3958, 5053
BstXI CCANNNNNNTGG 3 cut(s) 278, 345, 3393
BstYI RGATCY 5 cut(s) 1150, 1834, 2494, 3958, 5053
Bsu36I CCTNAGG 1 cut(s) 3322
BsuI GTATCC 4 cut(s) 1749, 1815, 3052, 4098
BtgI CCRYGG 2 cut(s) 2478, 3305
BtrI CACGTC 1 cut(s) 3812
BtsI GCAGTG 2 cut(s) 378, 5195
BtsIMutI CAGTG 7 cut(s) 276, 378, 1523, 1894, 3667, 4430, 5195
BtuMI TCGCGA 1 cut(s) 3235
BveI ACCTGC 2 cut(s) 1301, 1336
CaiI CAGNNNCTG 1 cut(s) 4466
CciI TCATGA 2 cut(s) 559, 4378
CfoI GCGC 3 cut(s) 417, 3329, 3792
Cfr10I RCCGGY 1 cut(s) 4412
Cfr13I GGNCC 5 cut(s) 588, 2475, 2984, 3599, 3758
Cfr42I CCGCGG 1 cut(s) 2481
CseI GACGC 1 cut(s) 4178
CspAI ACCGGT 1 cut(s) 4412
CspCI CAANNNNNGTGG 6 cut(s) 53, 88, 2002, 2037, 4399, 4434
DraI TTTAAA 3 cut(s) 1026, 3867, 4005
EaeI YGGCCR 2 cut(s) 541, 2085
Eam1104I CTCTTC 7 cut(s) 159, 2161, 2222, 2928, 3111, 3571, 3586
EarI CTCTTC 7 cut(s) 159, 2161, 2222, 2928, 3111, 3571, 3586
Ecl136II GAGCTC 1 cut(s) 382
Eco130I CCWWGG 5 cut(s) 1014, 3305, 4470, 4660, 5085
Eco24I GRGCYC 2 cut(s) 384, 4751
Eco31I GGTCTC 1 cut(s) 685
Eco32I GATATC 4 cut(s) 511, 1090, 1756, 3252
Eco47I GGWCC 2 cut(s) 2984, 3758
Eco53kI GAGCTC 1 cut(s) 382
Eco57I CTGAAG 5 cut(s) 1248, 2586, 3707, 4277, 4526
Eco81I CCTNAGG 1 cut(s) 3322
Eco88I CYCGRG 1 cut(s) 363
EcoICRI GAGCTC 1 cut(s) 382
EcoNI CCTNNNNNAGG 2 cut(s) 123, 4379
EcoRI GAATTC 2 cut(s) 1250, 4823
EcoRV GATATC 4 cut(s) 511, 1090, 1756, 3252
EcoT14I CCWWGG 5 cut(s) 1014, 3305, 4470, 4660, 5085
EcoT22I ATGCAT 4 cut(s) 193, 724, 1457, 2059
EcoT38I GRGCYC 2 cut(s) 384, 4751
ErhI CCWWGG 5 cut(s) 1014, 3305, 4470, 4660, 5085
Esp3I CGTCTC 3 cut(s) 44, 2102, 4578
FalI AAGNNNNNCTT 6 cut(s) 653, 685, 657, 689, 2267, 2299
FaqI GGGAC 2 cut(s) 2391, 3749
FauI CCCGC 2 cut(s) 2485, 3849
FauNDI CATATG 1 cut(s) 1408
FbaI TGATCA 2 cut(s) 1993, 3142
FriOI GRGCYC 2 cut(s) 384, 4751
GlaI GCGC 3 cut(s) 416, 3328, 3791
GsuI CTGGAG 6 cut(s) 314, 430, 2449, 2475, 2489, 5069
HaeII RGCGCY 1 cut(s) 418
HapII CCGG 8 cut(s) 260, 1112, 2194, 3341, 3676, 3920, 4413, 4514
HgaI GACGC 1 cut(s) 4178
HhaI GCGC 3 cut(s) 417, 3329, 3792
Hin6I GCGC 3 cut(s) 415, 3327, 3790
HinP1I GCGC 3 cut(s) 415, 3327, 3790
HincII GTYRAC 2 cut(s) 942, 1206
HindII GTYRAC 2 cut(s) 942, 1206
HindIII AAGCTT 1 cut(s) 5202
HpaII CCGG 8 cut(s) 260, 1112, 2194, 3341, 3676, 3920, 4413, 4514
Hpy166II GTNNAC 9 cut(s) 600, 942, 983, 1206, 1495, 1550, 3533, 3833, 4804
Hpy8I GTNNAC 9 cut(s) 600, 942, 983, 1206, 1495, 1550, 3533, 3833, 4804
Hpy99I CGWCG 2 cut(s) 3240, 4539
HpyCH4III ACNGT 4 cut(s) 946, 3040, 3391, 3662
HpyCH4IV ACGT 6 cut(s) 2095, 3811, 4504, 4534, 4768, 5041
HpySE526I ACGT 6 cut(s) 2095, 3811, 4504, 4534, 4768, 5041
HspAI GCGC 3 cut(s) 415, 3327, 3790
Kpn2I TCCGGA 2 cut(s) 1111, 3340
KpnI GGTACC 1 cut(s) 4410
Ksp22I TGATCA 2 cut(s) 1993, 3142
KspI CCGCGG 1 cut(s) 2481
MaeII ACGT 6 cut(s) 2095, 3811, 4504, 4534, 4768, 5041
MaeIII GTNAC 6 cut(s) 404, 515, 1635, 4024, 4481, 5156
MfeI CAATTG 2 cut(s) 1989, 2535
MflI RGATCY 5 cut(s) 1150, 1834, 2494, 3958, 5053
MhlI GDGCHC 4 cut(s) 247, 384, 1926, 4751
MlsI TGGCCA 1 cut(s) 543
MluNI TGGCCA 1 cut(s) 543
MlyI GAGTC 7 cut(s) 1522, 2147, 2165, 2525, 3420, 3557, 3731
MmeI TCCRAC 4 cut(s) 1689, 2707, 3346, 3410
Mox20I TGGCCA 1 cut(s) 543
Mph1103I ATGCAT 4 cut(s) 193, 724, 1457, 2059
MroI TCCGGA 2 cut(s) 1111, 3340
MroXI GAANNNNTTC 2 cut(s) 582, 668
MscI TGGCCA 1 cut(s) 543
MslI CAYNNNNRTG 4 cut(s) 281, 434, 611, 3660
Msp20I TGGCCA 1 cut(s) 543
MspA1I CMGCKG 3 cut(s) 2480, 3911, 4463
MspCI CTTAAG 1 cut(s) 2671
MspI CCGG 8 cut(s) 260, 1112, 2194, 3341, 3676, 3920, 4413, 4514
MunI CAATTG 2 cut(s) 1989, 2535
Mva1269I GAATGC 5 cut(s) 191, 1554, 1954, 2672, 3242
MvnI CGCG 3 cut(s) 2017, 2480, 3235
NciI CCSGG 5 cut(s) 260, 2195, 3677, 3920, 4515
NcoI CCATGG 1 cut(s) 3305
NdeI CATATG 1 cut(s) 1408
NheI GCTAGC 1 cut(s) 1186
NlaIV GGNNCC 9 cut(s) 590, 627, 751, 834, 1925, 3601, 4408, 4674, 4796
NmuCI GTSAC 4 cut(s) 404, 4024, 4481, 5156
NruI TCGCGA 1 cut(s) 3235
NsiI ATGCAT 4 cut(s) 193, 724, 1457, 2059
NspI RCATGY 7 cut(s) 191, 286, 1654, 2260, 2426, 3688, 4165
PaeI GCATGC 2 cut(s) 191, 286
PaeR7I CTCGAG 1 cut(s) 363
PagI TCATGA 2 cut(s) 559, 4378
PaqCI CACCTGC 1 cut(s) 1301
PasI CCCWGGG 1 cut(s) 1560
PciI ACATGT 2 cut(s) 1650, 2422
PctI GAATGC 5 cut(s) 191, 1554, 1954, 2672, 3242
PdmI GAANNNNTTC 2 cut(s) 582, 668
Pfl23II CGTACG 1 cut(s) 2092
PflFI GACNNNGTC 1 cut(s) 2195
PflMI CCANNNNNTGG 4 cut(s) 2310, 2356, 3781, 4451
PfoI TCCNGGA 5 cut(s) 1269, 3022, 4513, 4635, 5101
PinAI ACCGGT 1 cut(s) 4412
PleI GAGTC 7 cut(s) 1521, 2147, 2165, 2525, 3420, 3557, 3731
PpsI GAGTC 7 cut(s) 1521, 2147, 2165, 2525, 3420, 3557, 3731
PscI ACATGT 2 cut(s) 1650, 2422
PshBI ATTAAT 2 cut(s) 4175, 4842
PsiI TTATAA 1 cut(s) 3353
Psp124BI GAGCTC 1 cut(s) 384
Psp1406I AACGTT 1 cut(s) 4504
PspLI CGTACG 1 cut(s) 2092
PspN4I GGNNCC 9 cut(s) 590, 627, 751, 834, 1925, 3601, 4408, 4674, 4796
PspPI GGNCC 5 cut(s) 588, 2475, 2984, 3599, 3758
PspXI VCTCGAGB 1 cut(s) 363
PstI CTGCAG 2 cut(s) 1681, 3562
PstNI CAGNNNCTG 1 cut(s) 4466
PsuI RGATCY 5 cut(s) 1150, 1834, 2494, 3958, 5053
PsyI GACNNNGTC 1 cut(s) 2195
PvuII CAGCTG 1 cut(s) 4463
RruI TCGCGA 1 cut(s) 3235
RseI CAYNNNNRTG 4 cut(s) 281, 434, 611, 3660
SacI GAGCTC 1 cut(s) 384
SacII CCGCGG 1 cut(s) 2481
Sau96I GGNCC 5 cut(s) 588, 2475, 2984, 3599, 3758
ScaI AGTACT 2 cut(s) 824, 3610
SchI GAGTC 7 cut(s) 1522, 2147, 2165, 2525, 3420, 3557, 3731
SduI GDGCHC 4 cut(s) 247, 384, 1926, 4751
SfcI CTRYAG 3 cut(s) 1677, 3558, 4971
Sfr274I CTCGAG 1 cut(s) 363
Sfr303I CCGCGG 1 cut(s) 2481
SgrBI CCGCGG 1 cut(s) 2481
SinI GGWCC 2 cut(s) 2984, 3758
SlaI CTCGAG 1 cut(s) 363
SmiMI CAYNNNNRTG 4 cut(s) 281, 434, 611, 3660
SmlI CTYRAG 5 cut(s) 363, 383, 1817, 2671, 4099
SmoI CTYRAG 5 cut(s) 363, 383, 1817, 2671, 4099
SpeI ACTAGT 1 cut(s) 977
SphI GCATGC 2 cut(s) 191, 286
SsiI CCGC 8 cut(s) 681, 2017, 2084, 2478, 2480, 3617, 3856, 3909
SspI AATATT 5 cut(s) 1799, 2218, 3646, 3863, 4702
SstI GAGCTC 1 cut(s) 384
StyI CCWWGG 5 cut(s) 1014, 3305, 4470, 4660, 5085
TaaI ACNGT 4 cut(s) 946, 3040, 3391, 3662
TaiI ACGT 6 cut(s) 2098, 3814, 4507, 4537, 4771, 5044
TaqII GACCGA 1 cut(s) 1715
TatI WGTACW 4 cut(s) 597, 822, 1492, 3608
TauI GCSGC 4 cut(s) 683, 2087, 2483, 3911
TscAI CASTG 7 cut(s) 283, 378, 1523, 1901, 3667, 4437, 5195
TseFI GTSAC 4 cut(s) 404, 4024, 4481, 5156
Tsp45I GTSAC 4 cut(s) 404, 4024, 4481, 5156
TspGWI ACGGA 4 cut(s) 503, 2667, 4608, 4661
TspRI CASTG 7 cut(s) 283, 378, 1523, 1901, 3667, 4437, 5195
Tth111I GACNNNGTC 1 cut(s) 2195
Van91I CCANNNNNTGG 4 cut(s) 2310, 2356, 3781, 4451
Vha464I CTTAAG 1 cut(s) 2671
VpaK11BI GGWCC 2 cut(s) 2984, 3758
VspI ATTAAT 2 cut(s) 4175, 4842
XagI CCTNNNNNAGG 2 cut(s) 123, 4379
XapI RAATTY 9 cut(s) 1107, 1250, 1459, 1966, 2801, 3468, 3487, 3747, 4823
XceI RCATGY 7 cut(s) 191, 286, 1654, 2260, 2426, 3688, 4165
XcmI CCANNNNNNNNNTGG 1 cut(s) 610
XhoI CTCGAG 1 cut(s) 363
XmnI GAANNNNTTC 2 cut(s) 582, 668
ZrmI AGTACT 2 cut(s) 824, 3610
Zsp2I ATGCAT 4 cut(s) 193, 724, 1457, 2059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.