Rorug05G0119000

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0019936
Physical Location & Seq
Reverse (-)
10500937 .. 10501413
477 bp
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UTR
Exon/CDS
Intron
Rorug05G0119000.1

Sequence Viewer

Length: 477 bp
ATGGAATTTTGTAAGATTTCATCAGTCTGGATTTCTCTCGTAGCCTTATCCTTACTCCATTCCATTTATGCACAGGACAGAATCATAGACTTCCTGAACGGCCACAACTTTGCTCGAAGGGAGGTAGGTAACAAAGACCTGGAGTGGGATCCTGTACTAGAGCGTTATGCTACTGACTATGCCAAAAAACACATTCATGACTGCCAACTTATCCACACCGGTGGTCCATACGGTGAAAACCTTGCCTCGAGCCCTATTGAGATGTTCGGCTTAGATGCTGTGAGCATGTGGGTGTATGAGAAGAAAAATTACAACTACGAGACGAAATCTTGCGTCGGTGGCGAATGTGGGCATTATTTGCAGGTCATTTGGAATACCACGACTCATCTGGGGTGTGCCAAATTGAGGTGCAACAATGGGGGTACTTGGATCGGGTGCAACTATAATCCCCCTCCACAGGGAGACTTGCCTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

158

Amino Acids

17.95

Weight (kDa)

5.93

Isoelectric Point (pI)

38.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAP PF00188 32 - 148 1.6e-16 Cysteine-rich secretory protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019936)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0030641
rosa_multiflora Rmu_co8427705.1_g000001
rosa_roxburghii Rroxscaffold_2G00123230
rosa_rugosa Rorug05G0119000
rosa_samantha Rh5BG210300 Rh5CG234000 Rh5DG215300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 352
AclWI GGATC 3 cut(s) 143, 156, 437
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 1 cut(s) 5
AfaI GTAC 2 cut(s) 156, 424
AfiI CCNNNNNNNGG 4 cut(s) 145, 405, 457, 458
AgeI ACCGGT 1 cut(s) 218
AjnI CCWGG 1 cut(s) 138
AleI CACNNNNGTG 1 cut(s) 219
Alw26I GTCTC 2 cut(s) 314, 456
AlwI GGATC 3 cut(s) 143, 156, 437
Ama87I CYCGRG 1 cut(s) 247
AoxI GGCC 1 cut(s) 100
ApoI RAATTY 1 cut(s) 5
AsiGI ACCGGT 1 cut(s) 218
AspS9I GGNCC 1 cut(s) 224
AsuHPI GGTGA 1 cut(s) 245
AvaI CYCGRG 1 cut(s) 247
AvaII GGWCC 1 cut(s) 224
BamHI GGATCC 1 cut(s) 148
BanII GRGCYC 1 cut(s) 254
BarI GAAGNNNNNNTAC 2 cut(s) 293, 325
BceAI ACGGC 1 cut(s) 115
BciT130I CCWGG 1 cut(s) 140
BcoDI GTCTC 2 cut(s) 314, 456
BfaI CTAG 1 cut(s) 158
BfuAI ACCTGC 1 cut(s) 352
Bme1390I CCNGG 1 cut(s) 140
Bme18I GGWCC 1 cut(s) 224
BmeT110I CYCGRG 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 224
BmiI GGNNCC 1 cut(s) 150
BmrFI CCNGG 1 cut(s) 140
BmsI GCATC 1 cut(s) 265
BpmI CTGGAG 1 cut(s) 161
BsaWI WCCGGW 1 cut(s) 218
Bsc4I CCNNNNNNNGG 4 cut(s) 145, 405, 457, 458
Bse118I RCCGGY 1 cut(s) 218
BseBI CCWGG 1 cut(s) 140
BseLI CCNNNNNNNGG 4 cut(s) 145, 405, 457, 458
BshFI GGCC 1 cut(s) 102
BshTI ACCGGT 1 cut(s) 218
BsiHKCI CYCGRG 1 cut(s) 247
BsiSI CCGG 1 cut(s) 219
BslI CCNNNNNNNGG 4 cut(s) 145, 405, 457, 458
BsmAI GTCTC 2 cut(s) 314, 456
BsmBI CGTCTC 1 cut(s) 314
BsnI GGCC 1 cut(s) 102
BsoBI CYCGRG 1 cut(s) 247
Bsp1286I GDGCHC 1 cut(s) 254
Bsp143I GATC 2 cut(s) 148, 429
BspANI GGCC 1 cut(s) 102
BspHI TCATGA 1 cut(s) 196
BspLI GGNNCC 1 cut(s) 150
BspMI ACCTGC 1 cut(s) 352
BspPI GGATC 3 cut(s) 143, 156, 437
BsrFI RCCGGY 1 cut(s) 218
BssAI RCCGGY 1 cut(s) 218
BssMI GATC 2 cut(s) 148, 429
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 233
BstAPI GCANNNNNTGC 1 cut(s) 358
BstDEI CTNAG 1 cut(s) 271
BstKTI GATC 2 cut(s) 151, 432
BstMAI GTCTC 2 cut(s) 314, 456
BstMBI GATC 2 cut(s) 148, 429
BstMWI GCNNNNNNNGC 2 cut(s) 339, 358
BstNI CCWGG 1 cut(s) 140
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 1 cut(s) 138
BstX2I RGATCY 1 cut(s) 148
BstXI CCANNNNNNTGG 1 cut(s) 221
BstYI RGATCY 1 cut(s) 148
BsuRI GGCC 1 cut(s) 102
BveI ACCTGC 1 cut(s) 352
CciI TCATGA 1 cut(s) 196
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 1 cut(s) 224
CseI GACGC 1 cut(s) 322
Csp6I GTAC 2 cut(s) 155, 423
CspAI ACCGGT 1 cut(s) 218
CviAII CATG 2 cut(s) 197, 286
CviJI RGCY 4 cut(s) 44, 102, 252, 270
CviKI_1 RGCY 4 cut(s) 44, 102, 252, 270
CviQI GTAC 2 cut(s) 155, 423
DdeI CTNAG 1 cut(s) 271
DpnI GATC 2 cut(s) 150, 431
DpnII GATC 2 cut(s) 148, 429
EaeI YGGCCR 1 cut(s) 100
Eco24I GRGCYC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 224
Eco88I CYCGRG 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 138
EcoT38I GRGCYC 1 cut(s) 254
Esp3I CGTCTC 1 cut(s) 314
FaeI CATG 2 cut(s) 200, 289
FaiI YATR 9 cut(s) 69, 86, 168, 180, 198, 229, 287, 297, 444
FatI CATG 2 cut(s) 196, 285
FriOI GRGCYC 1 cut(s) 254
FspBI CTAG 1 cut(s) 158
GsuI CTGGAG 1 cut(s) 161
HaeIII GGCC 1 cut(s) 102
HapII CCGG 1 cut(s) 219
HgaI GACGC 1 cut(s) 322
Hin1II CATG 2 cut(s) 200, 289
HinfI GANTC 2 cut(s) 81, 382
HpaII CCGG 1 cut(s) 219
HphI GGTGA 1 cut(s) 245
Hpy188III TCNNGA 3 cut(s) 28, 94, 197
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 1 cut(s) 111
HpyCH4III ACNGT 1 cut(s) 233
HpyCH4V TGCA 4 cut(s) 71, 361, 411, 438
HpyF10VI GCNNNNNNNGC 2 cut(s) 339, 358
HpyF3I CTNAG 1 cut(s) 271
Hsp92II CATG 2 cut(s) 200, 289
Kzo9I GATC 2 cut(s) 148, 429
LweI GCATC 1 cut(s) 265
MaeI CTAG 1 cut(s) 158
MaeIII GTNAC 1 cut(s) 128
MalI GATC 2 cut(s) 150, 431
MboI GATC 2 cut(s) 148, 429
MboII GAAGA 1 cut(s) 313
MflI RGATCY 1 cut(s) 148
MhlI GDGCHC 1 cut(s) 254
MluCI AATT 3 cut(s) 5, 307, 401
MlyI GAGTC 1 cut(s) 376
MnlI CCTC 4 cut(s) 115, 256, 399, 462
MslI CAYNNNNRTG 3 cut(s) 195, 219, 290
MspI CCGG 1 cut(s) 219
MspR9I CCNGG 1 cut(s) 140
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 2 cut(s) 339, 358
NdeII GATC 2 cut(s) 148, 429
NlaIII CATG 2 cut(s) 200, 289
NlaIV GGNNCC 1 cut(s) 150
NspI RCATGY 1 cut(s) 289
OliI CACNNNNGTG 1 cut(s) 219
PaeR7I CTCGAG 1 cut(s) 247
PagI TCATGA 1 cut(s) 196
PfeI GAWTC 1 cut(s) 81
PinAI ACCGGT 1 cut(s) 218
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 1 cut(s) 150
PspPI GGNCC 1 cut(s) 224
PspXI VCTCGAGB 1 cut(s) 247
PsuI RGATCY 1 cut(s) 148
RsaI GTAC 2 cut(s) 156, 424
RsaNI GTAC 2 cut(s) 155, 423
RseI CAYNNNNRTG 3 cut(s) 195, 219, 290
Sau3AI GATC 2 cut(s) 148, 429
Sau96I GGNCC 1 cut(s) 224
SchI GAGTC 1 cut(s) 376
ScrFI CCNGG 1 cut(s) 140
SduI GDGCHC 1 cut(s) 254
SetI ASST 6 cut(s) 126, 130, 141, 243, 366, 410
SfaNI GCATC 1 cut(s) 265
Sfr274I CTCGAG 1 cut(s) 247
SgrAI CRCCGGYG 1 cut(s) 218
SinI GGWCC 1 cut(s) 224
SlaI CTCGAG 1 cut(s) 247
SmiMI CAYNNNNRTG 3 cut(s) 195, 219, 290
SmlI CTYRAG 1 cut(s) 247
SmoI CTYRAG 1 cut(s) 247
Sse9I AATT 3 cut(s) 5, 307, 401
SspMI CTAG 1 cut(s) 158
StyD4I CCNGG 1 cut(s) 138
TaaI ACNGT 1 cut(s) 233
TaqI TCGA 2 cut(s) 115, 248
TasI AATT 3 cut(s) 5, 307, 401
TatI WGTACW 1 cut(s) 154
TfiI GAWTC 1 cut(s) 81
TspDTI ATGAA 2 cut(s) 9, 185
VpaK11BI GGWCC 1 cut(s) 224
XapI RAATTY 1 cut(s) 5
XceI RCATGY 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 385
XhoI CTCGAG 1 cut(s) 247
XspI CTAG 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.