Rorug05G0206400

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0020728
Physical Location & Seq
Forward (+)
20597357 .. 20598425
1069 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0206400.1

Sequence Viewer

Length: 993 bp
ATGAAATGCACCGCAGTTTGCAAGTCATGGATGTCCCTGATCAAGTCCTCCACCTTCATTCGCCTCCACCTCAGCCGCACGATCCAAGACAACGACGCTCGCCTCCTTCTACTGAACGCGTTCTCCTCGTACCCGGAGATGAGTAGCAAGTCTTACGCTCTGCTCTGGGATGACCCTGTTTTTGCTCAGAGTCTCTTTGTCATTGGGACTTGTAACGGTCTCGTATGTCTTGCTCCTGACAGTTATGTTGATTCTCCCGTTTTAATTTGGAACCCATCTATTAGAAAGATTGTGATTTTGCCTAGCCCACCATCTAGTAGGAGTAGTGGTAAAAGATATGCTTTTGGTTACGATTCGCGTACCAATGACTATAAGGTGTTGATAATTATGATAGTTGAAACTCGAGGTGAGATTTACTCGCTAGCTAGGGGCACCTGGAAGAGTCTGAGTGCAGCTGCTACTCCTGTAGATTTTGTGCCTCCTGGAATGCATAATATTGCACGGGCATTGGTCAATGTCAATGGCTCTCCACATTGGTCTGGTGGAATGTATCGCCCCTGGGGAAACAGGGGCAACTGCATTATGTCGTTTGATATGGTCAGTGAAGTGTTTTGCAAGATAGAGGTGCCTGAAGCTTTGAGGGAACAGACATGCTGGGTTTCAAAACATGGGGACTGCCTCGCCTTGTATATGCTCGACAGGCTTTCCCCAAGGCCATCGTTTGACTTCAACATTTGGGTGATGAAAGAGTATGGTGTGGCGGAATCATGGACTAAGTTAACCCTACGGCAGGAGGGATATCTTTATATGCATGAATTCTTGTATTGTAGGCGTGAAGATCTCGTGTTCGAAAGATATAATGGAAATTTGCATACTGTGGATTGTAGGACTGGTCGAGTAAAAGATTATGGAATTCGTGCAGCGTACTACTTCATGGATTATTTTGTAGAGAGCATTCTCTTACTTGGCCAAGCCAATGCTATATCGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

330

Amino Acids

37.69

Weight (kDa)

8.45

Isoelectric Point (pI)

55.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 64 - 261 2.1e-16 F-box associated beta propeller domain
FBA_1 PF07734 65 - 262 9.2e-19 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020728)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000898.1_g000027
rosa_rugosa Rorug04G0459200 Rorug05G0206400
rosa_samantha Rh7AG292700 Rh7CG312800 Rh7DG296200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 431, 625
AccII CGCG 2 cut(s) 119, 358
AciI CCGC 3 cut(s) 12, 76, 761
AclWI GGATC 1 cut(s) 76
AcoI YGGCCR 1 cut(s) 967
AcsI RAATTY 3 cut(s) 815, 865, 912
AcuI CTGAAG 1 cut(s) 651
AfaI GTAC 4 cut(s) 131, 361, 926, 989
AfiI CCNNNNNNNGG 1 cut(s) 790
AflIII ACRYGT 1 cut(s) 117
AgsI TTSAA 3 cut(s) 398, 663, 730
AjnI CCWGG 3 cut(s) 434, 481, 557
AloI GAACNNNNNNTCC 2 cut(s) 107, 139
AluBI AGCT 3 cut(s) 425, 455, 635
AluI AGCT 3 cut(s) 425, 455, 635
Alw26I GTCTC 2 cut(s) 197, 224
AlwI GGATC 1 cut(s) 76
Ama87I CYCGRG 1 cut(s) 402
AoxI GGCC 2 cut(s) 713, 967
ApeKI GCWGC 3 cut(s) 452, 455, 920
ApoI RAATTY 3 cut(s) 815, 865, 912
ArsI GACNNNNNNTTYG 2 cut(s) 164, 196
Asp700I GAANNNNTTC 1 cut(s) 119
AsuC2I CCSGG 1 cut(s) 134
AsuHPI GGTGA 2 cut(s) 419, 751
AsuII TTCGAA 1 cut(s) 849
AsuNHI GCTAGC 1 cut(s) 421
AvaI CYCGRG 1 cut(s) 402
BaeGI GKGCMC 1 cut(s) 434
BalI TGGCCA 1 cut(s) 969
BanI GGYRCC 2 cut(s) 431, 625
BauI CACGAG 1 cut(s) 842
BbvCI CCTCAGC 1 cut(s) 71
BbvI GCAGC 3 cut(s) 442, 464, 932
BccI CCATC 3 cut(s) 283, 319, 724
BceAI ACGGC 1 cut(s) 803
BciT130I CCWGG 3 cut(s) 436, 483, 559
BclI TGATCA 1 cut(s) 39
BcnI CCSGG 1 cut(s) 134
BcoDI GTCTC 2 cut(s) 197, 224
BfaI CTAG 4 cut(s) 303, 315, 422, 426
BfmI CTRYAG 1 cut(s) 465
BglII AGATCT 1 cut(s) 838
BisI GCNGC 4 cut(s) 76, 453, 456, 921
BlsI GCNGC 4 cut(s) 77, 454, 457, 922
Bme1390I CCNGG 4 cut(s) 134, 436, 483, 559
BmeT110I CYCGRG 1 cut(s) 402
BmiI GGNNCC 3 cut(s) 272, 433, 627
BmrFI CCNGG 4 cut(s) 134, 436, 483, 559
BmtI GCTAGC 1 cut(s) 425
BplI GAGNNNNNCTC 2 cut(s) 401, 433
Bpu10I CCTNAGC 1 cut(s) 71
Bpu14I TTCGAA 1 cut(s) 849
BpuMI CCSGG 1 cut(s) 134
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 3 cut(s) 557, 558, 710
BsaXI ACNNNNNCTCC 4 cut(s) 107, 137, 313, 343
Bsc4I CCNNNNNNNGG 1 cut(s) 790
Bse1I ACTGG 1 cut(s) 895
BseBI CCWGG 3 cut(s) 436, 483, 559
BseDI CCNNGG 3 cut(s) 557, 558, 710
BseGI GGATG 2 cut(s) 36, 175
BseLI CCNNNNNNNGG 1 cut(s) 790
BseMII CTCAG 3 cut(s) 85, 200, 437
BseNI ACTGG 1 cut(s) 895
BseRI GAGGAG 1 cut(s) 115
BseSI GKGCMC 1 cut(s) 434
BseXI GCAGC 3 cut(s) 442, 464, 932
BseYI CCCAGC 1 cut(s) 654
BsgI GTGCAG 2 cut(s) 471, 939
Bsh1236I CGCG 2 cut(s) 119, 358
BshFI GGCC 2 cut(s) 715, 969
BshNI GGYRCC 2 cut(s) 431, 625
BsiHKCI CYCGRG 1 cut(s) 402
BsiSI CCGG 1 cut(s) 134
BslFI GGGAC 3 cut(s) 19, 220, 686
BslI CCNNNNNNNGG 1 cut(s) 790
BsmAI GTCTC 2 cut(s) 197, 224
BsmFI GGGAC 3 cut(s) 19, 220, 686
BsmI GAATGC 2 cut(s) 492, 954
BsnI GGCC 2 cut(s) 715, 969
Bso31I GGTCTC 1 cut(s) 224
BsoBI CYCGRG 1 cut(s) 402
Bsp119I TTCGAA 1 cut(s) 849
Bsp1286I GDGCHC 1 cut(s) 434
Bsp143I GATC 3 cut(s) 39, 81, 838
BspACI CCGC 3 cut(s) 12, 76, 761
BspANI GGCC 2 cut(s) 715, 969
BspCNI CTCAG 3 cut(s) 84, 199, 438
BspFNI CGCG 2 cut(s) 119, 358
BspLI GGNNCC 3 cut(s) 272, 433, 627
BspOI GCTAGC 1 cut(s) 425
BspPI GGATC 1 cut(s) 76
BspT104I TTCGAA 1 cut(s) 849
BspT107I GGYRCC 2 cut(s) 431, 625
BspTNI GGTCTC 1 cut(s) 224
BsrI ACTGG 1 cut(s) 895
BssECI CCNNGG 3 cut(s) 557, 558, 710
BssMI GATC 3 cut(s) 39, 81, 838
BssSI CACGAG 1 cut(s) 842
BssT1I CCWWGG 1 cut(s) 710
Bst2BI CACGAG 1 cut(s) 842
Bst2UI CCWGG 3 cut(s) 436, 483, 559
Bst4CI ACNGT 3 cut(s) 218, 242, 877
Bst6I CTCTTC 1 cut(s) 434
BstBI TTCGAA 1 cut(s) 849
BstC8I GCNNGC 2 cut(s) 100, 423
BstDEI CTNAG 4 cut(s) 71, 186, 446, 774
BstENI CCTNNNNNAGG 1 cut(s) 788
BstF5I GGATG 2 cut(s) 36, 175
BstFNI CGCG 2 cut(s) 119, 358
BstKTI GATC 3 cut(s) 42, 84, 841
BstMAI GTCTC 2 cut(s) 197, 224
BstMBI GATC 3 cut(s) 39, 81, 838
BstMWI GCNNNNNNNGC 1 cut(s) 700
BstNI CCWGG 3 cut(s) 436, 483, 559
BstNSI RCATGY 1 cut(s) 654
BstSCI CCNGG 4 cut(s) 132, 434, 481, 557
BstSFI CTRYAG 1 cut(s) 465
BstSLI GKGCMC 1 cut(s) 434
BstUI CGCG 2 cut(s) 119, 358
BstV1I GCAGC 3 cut(s) 442, 464, 932
BstX2I RGATCY 1 cut(s) 838
BstYI RGATCY 1 cut(s) 838
BsuRI GGCC 2 cut(s) 715, 969
BtsCI GGATG 2 cut(s) 36, 175
BtsIMutI CAGTG 1 cut(s) 607
Cac8I GCNNGC 2 cut(s) 100, 423
CseI GACGC 1 cut(s) 104
Csp6I GTAC 4 cut(s) 130, 360, 925, 988
CviAII CATG 6 cut(s) 27, 651, 668, 768, 812, 934
CviQI GTAC 4 cut(s) 130, 360, 925, 988
DdeI CTNAG 4 cut(s) 71, 186, 446, 774
DpnI GATC 3 cut(s) 41, 83, 840
DpnII GATC 3 cut(s) 39, 81, 838
EaeI YGGCCR 1 cut(s) 967
Eam1104I CTCTTC 1 cut(s) 434
EarI CTCTTC 1 cut(s) 434
EciI GGCGGA 1 cut(s) 776
Eco130I CCWWGG 1 cut(s) 710
Eco31I GGTCTC 1 cut(s) 224
Eco32I GATATC 1 cut(s) 800
Eco57I CTGAAG 1 cut(s) 651
Eco88I CYCGRG 1 cut(s) 402
EcoNI CCTNNNNNAGG 1 cut(s) 788
EcoRI GAATTC 2 cut(s) 815, 912
EcoRII CCWGG 3 cut(s) 434, 481, 557
EcoRV GATATC 1 cut(s) 800
EcoT14I CCWWGG 1 cut(s) 710
EcoT22I ATGCAT 2 cut(s) 492, 813
ErhI CCWWGG 1 cut(s) 710
FaeI CATG 6 cut(s) 30, 654, 671, 771, 815, 937
FalI AAGNNNNNCTT 2 cut(s) 325, 357
FaqI GGGAC 3 cut(s) 19, 220, 686
FatI CATG 6 cut(s) 26, 650, 667, 767, 811, 933
FbaI TGATCA 1 cut(s) 39
Fnu4HI GCNGC 4 cut(s) 76, 453, 456, 921
FokI GGATG 2 cut(s) 43, 182
Fsp4HI GCNGC 4 cut(s) 76, 453, 456, 921
FspBI CTAG 4 cut(s) 303, 315, 422, 426
GluI GCNGC 4 cut(s) 76, 453, 456, 921
GsaI CCCAGC 1 cut(s) 658
HaeIII GGCC 2 cut(s) 715, 969
HapII CCGG 1 cut(s) 134
HgaI GACGC 1 cut(s) 104
Hin1II CATG 6 cut(s) 30, 654, 671, 771, 815, 937
HincII GTYRAC 1 cut(s) 780
HindII GTYRAC 1 cut(s) 780
HindIII AAGCTT 1 cut(s) 633
HinfI GANTC 5 cut(s) 190, 251, 353, 442, 764
HpaI GTTAAC 1 cut(s) 780
HpaII CCGG 1 cut(s) 134
HphI GGTGA 2 cut(s) 419, 751
Hpy166II GTNNAC 1 cut(s) 780
Hpy188I TCNGA 2 cut(s) 189, 447
Hpy188III TCNNGA 1 cut(s) 236
Hpy8I GTNNAC 1 cut(s) 780
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 2 cut(s) 64, 116
HpyCH4III ACNGT 3 cut(s) 218, 242, 877
HpyF10VI GCNNNNNNNGC 1 cut(s) 700
HpyF3I CTNAG 4 cut(s) 71, 186, 446, 774
Hsp92II CATG 6 cut(s) 30, 654, 671, 771, 815, 937
Ksp22I TGATCA 1 cut(s) 39
KspAI GTTAAC 1 cut(s) 780
Kzo9I GATC 3 cut(s) 39, 81, 838
LmnI GCTCC 1 cut(s) 238
Lsp1109I GCAGC 3 cut(s) 442, 464, 932
MaeI CTAG 4 cut(s) 303, 315, 422, 426
MaeIII GTNAC 2 cut(s) 212, 347
MalI GATC 3 cut(s) 41, 83, 840
MboI GATC 3 cut(s) 39, 81, 838
MboII GAAGA 2 cut(s) 451, 848
MflI RGATCY 1 cut(s) 838
MhlI GDGCHC 1 cut(s) 434
MlsI TGGCCA 1 cut(s) 969
MluCI AATT 5 cut(s) 264, 384, 815, 865, 912
MluI ACGCGT 1 cut(s) 117
MluNI TGGCCA 1 cut(s) 969
MlyI GAGTC 2 cut(s) 199, 451
Mox20I TGGCCA 1 cut(s) 969
Mph1103I ATGCAT 2 cut(s) 492, 813
MroXI GAANNNNTTC 1 cut(s) 119
MscI TGGCCA 1 cut(s) 969
MseI TTAA 2 cut(s) 263, 779
MslI CAYNNNNRTG 1 cut(s) 737
Msp20I TGGCCA 1 cut(s) 969
MspA1I CMGCKG 1 cut(s) 455
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 4 cut(s) 134, 436, 483, 559
Mva1269I GAATGC 2 cut(s) 492, 954
MvaI CCWGG 3 cut(s) 436, 483, 559
MvnI CGCG 2 cut(s) 119, 358
MwoI GCNNNNNNNGC 1 cut(s) 700
NciI CCSGG 1 cut(s) 134
NdeII GATC 3 cut(s) 39, 81, 838
NheI GCTAGC 1 cut(s) 421
NlaIII CATG 6 cut(s) 30, 654, 671, 771, 815, 937
NlaIV GGNNCC 3 cut(s) 272, 433, 627
NsiI ATGCAT 2 cut(s) 492, 813
NspI RCATGY 1 cut(s) 654
NspV TTCGAA 1 cut(s) 849
PaeR7I CTCGAG 1 cut(s) 402
PasI CCCWGGG 1 cut(s) 558
PctI GAATGC 2 cut(s) 492, 954
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 3 cut(s) 251, 353, 764
PfoI TCCNGGA 1 cut(s) 481
PkrI GCNGC 4 cut(s) 77, 454, 457, 922
PleI GAGTC 2 cut(s) 198, 450
PpsI GAGTC 2 cut(s) 198, 450
Psp6I CCWGG 3 cut(s) 434, 481, 557
PspFI CCCAGC 1 cut(s) 654
PspGI CCWGG 3 cut(s) 434, 481, 557
PspN4I GGNNCC 3 cut(s) 272, 433, 627
PspXI VCTCGAGB 1 cut(s) 402
PsuI RGATCY 1 cut(s) 838
PvuII CAGCTG 1 cut(s) 455
RsaI GTAC 4 cut(s) 131, 361, 926, 989
RsaNI GTAC 4 cut(s) 130, 360, 925, 988
RseI CAYNNNNRTG 1 cut(s) 737
SaqAI TTAA 2 cut(s) 263, 779
SatI GCNGC 4 cut(s) 76, 453, 456, 921
Sau3AI GATC 3 cut(s) 39, 81, 838
SchI GAGTC 2 cut(s) 199, 451
ScrFI CCNGG 4 cut(s) 134, 436, 483, 559
SduI GDGCHC 1 cut(s) 434
SetI ASST 9 cut(s) 56, 72, 378, 409, 427, 437, 457, 627, 637
SfcI CTRYAG 1 cut(s) 465
Sfr274I CTCGAG 1 cut(s) 402
SfuI TTCGAA 1 cut(s) 849
SlaI CTCGAG 1 cut(s) 402
SmiMI CAYNNNNRTG 1 cut(s) 737
SmlI CTYRAG 1 cut(s) 402
SmoI CTYRAG 1 cut(s) 402
Sse9I AATT 5 cut(s) 264, 384, 815, 865, 912
SsiI CCGC 3 cut(s) 12, 76, 761
SspI AATATT 1 cut(s) 496
SspMI CTAG 4 cut(s) 303, 315, 422, 426
StyD4I CCNGG 4 cut(s) 132, 434, 481, 557
StyI CCWWGG 1 cut(s) 710
TaaI ACNGT 3 cut(s) 218, 242, 877
TaqI TCGA 4 cut(s) 403, 696, 849, 895
TasI AATT 5 cut(s) 264, 384, 815, 865, 912
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 3 cut(s) 251, 353, 764
Tru1I TTAA 2 cut(s) 263, 779
Tru9I TTAA 2 cut(s) 263, 779
TscAI CASTG 1 cut(s) 607
TseI GCWGC 3 cut(s) 452, 455, 920
TspDTI ATGAA 5 cut(s) 17, 46, 758, 828, 922
TspRI CASTG 1 cut(s) 607
XagI CCTNNNNNAGG 1 cut(s) 788
XapI RAATTY 3 cut(s) 815, 865, 912
XceI RCATGY 1 cut(s) 654
XhoI CTCGAG 1 cut(s) 402
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 4 cut(s) 303, 315, 422, 426
Zsp2I ATGCAT 2 cut(s) 492, 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.