Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0010727

Physical Location & Seq

Genomic Coordinates Reverse (-)

51079321 .. 51084872

Gene Structure 5552 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0377400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2436 bp

ATGGATTTCGTGGAATTGGAAGCCATAGAAGGCCTCCGATGGTCCTGGAACTCCTGGCCGTCTTCCAAAGCCGACCAAGCCGCTCTCGTCATCCCCCTAAGCGTCATGTGCACGCCGCTGATGCAACAAGCCGAGCTCCCACTCCTCCCCTACGACCCCGTCACCTGCTCCCAATGCGGCGCCGTTTTGAACCCCTACGCGCGCGTCGACTACACCTCCCGCATCTGGTTCTGCTCCTTGTGCCTCCACAAGAACCCCTTCCCTCACTCCTACTCCAACATCGGCGACACCAATCTCCCCGCCGAGCTCTTCCCCACTTACAGCGCCGTCGAGTACGCCCGAACCGCCCCGAGTCCCGCCCCCGCCCACAATTGGCCCAACGGCGGCCTCTCGTCCTCGTCCTCGCTGTCCTCGATGGTGTCGTCGTCGCCAAACTCCGGGTCGGACGATTCGCGAGGGATCCGACCCGCGTTTGTGTTGGTCGTCGACGTGGCCACGCCCCGGGATGAGCTGGCGGCTCTGAAGAATGAGCTGCTGCTTGTGCTGGAGCAACTGCCGGAGACTGCTCTGGTGGGTTTAGTCACGTTTGATTCCATGGTGCGCCTTCATGATCTCGAGTTTTCCGAGTGCTCCAGAGTCGTTGTGCTTCACGGAGATCGGCAGCTCTCATCGCTTCAGATTCAACAATTTCTGGGCATTAATAGGCCTAGGCAGCAGCAACTCGGAAAAATGCCTTTTTCCCAAAAGCAAGGGTTCTTGCTACCAGTATCTGAGTGTGAGTTCAACATTTCAACTGCAATTGAAGAAATCCAGTCTTCGGCAGTCAAGCCTGGTCATCGCCCAGGAAGGTCAACAGGAGCAGCAATTTCAGCCGCACTTGGACTTTTGGAAGGATGCTTGGTGAGCACTGGCTCCAGAATCATGGTCTTCACATCTGGACCTGCAACTGTAGGCCCAGGAATTATTGTAGACTCTGATCTTGGTTATTCCATCAGAACTCACCGAGATCTCATCAATGGTCATGCTCCTTACTATAGTAGATCTTGCAGCTTCTACAGACAAGTGTCACAGAGGTTAACTGATGCATCTATTGTCCTCGATTTGTTTGCTTGTTCTCTTGATCAAGCTGGCTCTGCAGAGCTTAAGGATCCTGTTGAGAGATCGGGTGGTTTCATGATGCTAGGCGAGTCCTTTGAGTCGAATCAGTTCAGAAAATGTTTGCGGCACATTTTTACTCGTGATGAAGAGGGGTATTTGAAGATGTATTTTGATGCAACTATTGAGATAGTGACCACTAAAGATGTTAAAATCTGTGGAGCCCTAGGTCCTTGTGTATCTCTTCGGAAAGTTAACAATTTAGTGAGTAGCACTGAGATTGGTGAGGGTGGTACCTATATATGGAAGTTAGGTAGTATTACCAATAAAACATGCATCGCCTTTTTCTTTGAAGTTAGTGATGAGCAGAAAGTTCAACTTGGCTCGGCATTCTTTATACAGTTCATAACACGATACCGACATGGGAACATGGGAATCCGGAAACGGGTGACAACTGCTGCAAGAAGATGGGTTGGTAACCGTTCACCAGAAATTGCTGCTGGGTTCGATCAAGAAACAGCTGCTTCTGTGATAGCCAGGCTTGCTATTCACCGAGCTGAGAATTGTTTTGCTCGAGATGTTATTGGATGGCTGGATGACACGTTGATCCATTTTGCTTCAAAATTTGGAGACTATGTGGAGGAAGATCCATCTTCTTTCCGCTTGGCATCCAACTTCTCTCTTTTTCCCCAATTCATGTATTACTTGAGGAGGTCTCAGTTTATTGATGTCTTTAATAGTAGTCCTGATGAGACAGCTTTCTTTCGGCTGATGCTGAACCGTGAGGGCGTGGTGGGTTCTCTTATCATGATCCAGCCTACACTTCTCCAATATTCCTTTGACGGTCCACCCATTCCAGTCCTCCTGGATGTTCGCTCTATCTCTCCAGATGTTATCTTGCTCTTTGATTCTTACTTTCATGTGGTTATTCACTATGGGTCCAAGATTGCTCAATGGAGGAAGCTTGGTTATGATAAGGATCCAAATCATGAGAATTTGAGGAAGCTATTGGAAGCCCCAGAAATTGATGCACAGCAGTTGGTGGCTGACCGTGTTCCTGCACCGAAGCTCATTAAATGTGACCAGCATGGTAGCCAGATGATGATTGCTAGTTTGTTATGGGATAGGCGCTACTTCAGGATCCATTGGAGGAAAATGTTAACCAACTTTGTTGAAGAGCTTCAAAGATGGTTGCTCCATGTGAATTCCACATGTGAAAGTCAAACATTTGGGAAAGATTTCCGACTGGGCAAACTGATATTGACACAGGTGAATATGATTATGCAATGTAATGGCTCGTTTCTGGACTGGACGTACGACAGAACTGGCGATTGCTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005351 GO:0005355 GO:0005402 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0008643 GO:0008645 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0015075 GO:0015077 GO:0015078 GO:0015144 GO:0015145 GO:0015149 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015318 GO:0015672 GO:0015749 GO:0022804 GO:0022857 GO:0022890 GO:0031967 GO:0031975 GO:0034219 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046323 GO:0051119 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0098655 GO:0098660 GO:0098662 GO:1902600 GO:1904659

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

811

Amino Acids

90.87

Weight (kDa)

6.09

Isoelectric Point (pI)

50.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-Sec23_Sec24	PF04810	53 - 91	1.5e-13	Sec23/Sec24 zinc finger
Sec23_trunk	PF04811	156 - 411	6.3e-50	Sec23/Sec24 trunk domain
Sec23_BS	PF08033	423 - 523	6.9e-19	Sec23/Sec24 beta-sandwich domain
Sec23_helical	PF04815	535 - 632	1.1e-23	Sec23/Sec24 helical domain
Gelsolin	PF00626	649 - 734	1.9e-07	Gelsolin repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010727)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G59250
fragaria_vesca	FvH4_3g36930 FvH4_3g36930 FvH4_3g36930
malus_domestica	MD11G1095200.v1.1
prunus_persica	Prupe.6G070700_v2.0.a1 Prupe.6G070700_v2.0.a1
pyrus_communis	pycom11g08070
rosa_chinensis	RchiOBHm_Chr5g0066331
rosa_laevigata	RLG00000035851
rosa_multiflora	Rmu_sc0006887.1_g000007
rosa_roxburghii	Rroxscaffold_1G00014800
rosa_rugosa	Rorug05G0377300 Rorug05G0377400 Rorug05G0377400
rosa_samantha	Rh5BG452100 Rh5BG452200 Rh5CG474400
rosa_wichuraiana	Rw5G040810 Rw5G040820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	173
Acc36I	ACCTGC	2 cut(s)	173, 949
Acc65I	GGTACC	1 cut(s)	1388
AccB1I	GGYRCC	2 cut(s)	179, 1388
AccBSI	CCGCTC	1 cut(s)	83
AccI	GTMKAC	3 cut(s)	207, 486, 969
AccII	CGCG	5 cut(s)	200, 202, 204, 454, 470
AccIII	TCCGGA	1 cut(s)	1533
AcoI	YGGCCR	2 cut(s)	56, 492
AcsI	RAATTY	3 cut(s)	1718, 2089, 2299
AcuI	CTGAAG	3 cut(s)	542, 659, 2215
AcyI	GRCGYC	1 cut(s)	180
AfaI	GTAC	3 cut(s)	335, 1390, 2411
AfiI	CCNNNNNNNGG	8 cut(s)	225, 372, 383, 437, 501, 817, 1398, 1540
AflII	CTTAAG	1 cut(s)	1142
AflIII	ACRYGT	2 cut(s)	1695, 2306
AjiI	CACGTC	1 cut(s)	490
AjnI	CCWGG	7 cut(s)	44, 53, 829, 841, 955, 1631, 1959
Alw21I	GWGCWC	5 cut(s)	113, 138, 309, 632, 908
Alw26I	GTCTC	4 cut(s)	554, 1719, 1815, 1841
Alw44I	GTGCAC	1 cut(s)	109
AlwNI	CAGNNNCTG	1 cut(s)	770
Ama87I	CYCGRG	4 cut(s)	349, 501, 614, 1668
Aor13HI	TCCGGA	1 cut(s)	1533
AoxI	GGCC	7 cut(s)	31, 56, 374, 385, 492, 704, 952
ApaLI	GTGCAC	1 cut(s)	109
ApoI	RAATTY	3 cut(s)	1718, 2089, 2299
ArsI	GACNNNNNNTTYG	2 cut(s)	425, 457
AseI	ATTAAT	1 cut(s)	699
Asp700I	GAANNNNTTC	4 cut(s)	257, 1205, 2274, 2333
Asp718I	GGTACC	1 cut(s)	1388
AspA2I	CCTAGG	2 cut(s)	707, 1321
AspLEI	GCGC	6 cut(s)	182, 202, 204, 326, 603, 2226
AspS9I	GGNCC	7 cut(s)	42, 375, 938, 953, 1325, 1940, 2034
AsuC2I	CCSGG	3 cut(s)	439, 502, 503
AsuHPI	GGTGA	8 cut(s)	154, 913, 992, 1391, 1555, 1572, 1637, 2377
AvaI	CYCGRG	4 cut(s)	349, 501, 614, 1668
AvaII	GGWCC	5 cut(s)	42, 938, 1325, 1940, 2034
AvrII	CCTAGG	2 cut(s)	707, 1321
BaeGI	GKGCMC	1 cut(s)	113
BalI	TGGCCA	1 cut(s)	494
BamHI	GGATCC	4 cut(s)	459, 1147, 2074, 2235
BanI	GGYRCC	2 cut(s)	179, 1388
BanII	GRGCYC	3 cut(s)	138, 309, 1321
BauI	CACGAG	1 cut(s)	1236
BbsI	GAAGAC	3 cut(s)	54, 807, 919
Bbv12I	GWGCWC	5 cut(s)	113, 138, 309, 632, 908
BccI	CCATC	7 cut(s)	33, 409, 998, 1557, 1677, 1753, 2277
BceAI	ACGGC	4 cut(s)	43, 167, 311, 397
BcgI	CGANNNNNNTGC	2 cut(s)	1088, 1122
BciT130I	CCWGG	7 cut(s)	46, 55, 831, 843, 957, 1633, 1961
BclI	TGATCA	1 cut(s)	1120
BcnI	CCSGG	3 cut(s)	439, 502, 503
BcoDI	GTCTC	4 cut(s)	554, 1719, 1815, 1841
BfaI	CTAG	4 cut(s)	708, 1181, 1322, 2205
BfmI	CTRYAG	4 cut(s)	948, 1033, 1054, 1134
BfoI	RGCGCY	3 cut(s)	183, 327, 2227
BfrI	CTTAAG	1 cut(s)	1142
BfuAI	ACCTGC	2 cut(s)	173, 949
BglII	AGATCT	2 cut(s)	1006, 1040
BlnI	CCTAGG	2 cut(s)	707, 1321
Bme18I	GGWCC	5 cut(s)	42, 938, 1325, 1940, 2034
BmeT110I	CYCGRG	4 cut(s)	349, 501, 614, 1668
BmgBI	CACGTC	1 cut(s)	490
BmgT120I	GGNCC	7 cut(s)	42, 375, 938, 953, 1325, 1940, 2034
BmiI	GGNNCC	9 cut(s)	181, 461, 913, 1149, 1318, 1390, 2035, 2076, 2237
BmrI	ACTGGG	1 cut(s)	2351
BmuI	ACTGGG	1 cut(s)	2351
BoxI	GACNNNNGTC	1 cut(s)	1063
BpiI	GAAGAC	3 cut(s)	54, 807, 919
BplI	GAGNNNNNCTC	2 cut(s)	1795, 1827
BpmI	CTGGAG	4 cut(s)	566, 616, 898, 1965
Bpu10I	CCTNAGC	1 cut(s)	98
BpuEI	CTTGAG	1 cut(s)	1822
BpuMI	CCSGG	3 cut(s)	439, 502, 503
BsaBI	GATNNNNATC	2 cut(s)	2073, 2079
BsaHI	GRCGYC	1 cut(s)	180
BsaI	GGTCTC	1 cut(s)	1815
BsaJI	CCNNGG	7 cut(s)	500, 501, 594, 707, 841, 955, 1321
BsaWI	WCCGGW	1 cut(s)	1533
BsaXI	ACNNNNNCTCC	6 cut(s)	200, 230, 257, 287, 1716, 1746
Bsc4I	CCNNNNNNNGG	8 cut(s)	225, 372, 383, 437, 501, 817, 1398, 1540
Bse1I	ACTGG	7 cut(s)	764, 811, 913, 1952, 2346, 2408, 2425
Bse3DI	GCAATG	1 cut(s)	2387
Bse8I	GATNNNNATC	2 cut(s)	2073, 2079
BseAI	TCCGGA	1 cut(s)	1533
BseBI	CCWGG	7 cut(s)	46, 55, 831, 843, 957, 1633, 1961
BseDI	CCNNGG	7 cut(s)	500, 501, 594, 707, 841, 955, 1321
BseGI	GGATG	7 cut(s)	90, 511, 899, 1688, 1696, 1763, 1969
BseJI	GATNNNNATC	2 cut(s)	2073, 2079
BseLI	CCNNNNNNNGG	8 cut(s)	225, 372, 383, 437, 501, 817, 1398, 1540
BseMI	GCAATG	1 cut(s)	2387
BseMII	CTCAG	4 cut(s)	762, 1362, 1644, 1826
BseNI	ACTGG	7 cut(s)	764, 811, 913, 1952, 2346, 2408, 2425
BsePI	GCGCGC	1 cut(s)	200
BseRI	GAGGAG	2 cut(s)	134, 1819
BseSI	GKGCMC	1 cut(s)	113
BseYI	CCCAGC	1 cut(s)	1595
BsgI	GTGCAG	1 cut(s)	2139
Bsh1236I	CGCG	5 cut(s)	200, 202, 204, 454, 470
BshFI	GGCC	7 cut(s)	33, 58, 376, 387, 494, 706, 954
BshNI	GGYRCC	2 cut(s)	179, 1388
BsiHKAI	GWGCWC	5 cut(s)	113, 138, 309, 632, 908
BsiHKCI	CYCGRG	4 cut(s)	349, 501, 614, 1668
BsiSI	CCGG	4 cut(s)	438, 502, 557, 1534
BsiWI	CGTACG	1 cut(s)	2409
BslFI	GGGAC	1 cut(s)	339
BslI	CCNNNNNNNGG	8 cut(s)	225, 372, 383, 437, 501, 817, 1398, 1540
BsmAI	GTCTC	4 cut(s)	554, 1719, 1815, 1841
BsmFI	GGGAC	1 cut(s)	339
BsmI	GAATGC	1 cut(s)	1484
BsnI	GGCC	7 cut(s)	33, 58, 376, 387, 494, 706, 954
Bso31I	GGTCTC	1 cut(s)	1815
BsoBI	CYCGRG	4 cut(s)	349, 501, 614, 1668
Bsp1286I	GDGCHC	6 cut(s)	113, 138, 309, 632, 908, 1321
Bsp13I	TCCGGA	1 cut(s)	1533
Bsp19I	CCATGG	1 cut(s)	594
Bsp68I	TCGCGA	1 cut(s)	454
BspANI	GGCC	7 cut(s)	33, 58, 376, 387, 494, 706, 954
BspCNI	CTCAG	4 cut(s)	763, 1363, 1645, 1825
BspEI	TCCGGA	1 cut(s)	1533
BspFNI	CGCG	5 cut(s)	200, 202, 204, 454, 470
BspHI	TCATGA	4 cut(s)	607, 1173, 1902, 2083
BspLI	GGNNCC	9 cut(s)	181, 461, 913, 1149, 1318, 1390, 2035, 2076, 2237
BspMAI	CTGCAG	1 cut(s)	1138
BspMI	ACCTGC	2 cut(s)	173, 949
BspQI	GCTCTTC	2 cut(s)	314, 2265
BspT107I	GGYRCC	2 cut(s)	179, 1388
BspTI	CTTAAG	1 cut(s)	1142
BspTNI	GGTCTC	1 cut(s)	1815
BsrBI	CCGCTC	1 cut(s)	83
BsrDI	GCAATG	1 cut(s)	2387
BsrI	ACTGG	7 cut(s)	764, 811, 913, 1952, 2346, 2408, 2425
BssECI	CCNNGG	7 cut(s)	500, 501, 594, 707, 841, 955, 1321
BssHII	GCGCGC	1 cut(s)	200
BssNI	GRCGYC	1 cut(s)	180
BssSI	CACGAG	1 cut(s)	1236
BssT1I	CCWWGG	3 cut(s)	594, 707, 1321
Bst2BI	CACGAG	1 cut(s)	1236
Bst2UI	CCWGG	7 cut(s)	46, 55, 831, 843, 957, 1633, 1961
Bst4CI	ACNGT	6 cut(s)	949, 1497, 1577, 1877, 1940, 2147
Bst6I	CTCTTC	4 cut(s)	314, 1239, 1344, 2265
BstACI	GRCGYC	1 cut(s)	180
BstAFI	CTTAAG	1 cut(s)	1142
BstC8I	GCNNGC	5 cut(s)	113, 202, 513, 1129, 1638
BstDEI	CTNAG	5 cut(s)	98, 771, 1371, 1653, 1812
BstDSI	CCRYGG	1 cut(s)	594
BstEII	GGTNACC	1 cut(s)	1571
BstF5I	GGATG	7 cut(s)	90, 511, 899, 1688, 1696, 1763, 1969
BstFNI	CGCG	5 cut(s)	200, 202, 204, 454, 470
BstH2I	RGCGCY	3 cut(s)	183, 327, 2227
BstHHI	GCGC	6 cut(s)	182, 202, 204, 326, 603, 2226
BstMAI	GTCTC	4 cut(s)	554, 1719, 1815, 1841
BstNI	CCWGG	7 cut(s)	46, 55, 831, 843, 957, 1633, 1961
BstNSI	RCATGY	2 cut(s)	1431, 2310
BstPAI	GACNNNNGTC	1 cut(s)	1063
BstPI	GGTNACC	1 cut(s)	1571
BstSFI	CTRYAG	4 cut(s)	948, 1033, 1054, 1134
BstSLI	GKGCMC	1 cut(s)	113
BstUI	CGCG	5 cut(s)	200, 202, 204, 454, 470
BstV2I	GAAGAC	3 cut(s)	54, 807, 919
BstX2I	RGATCY	7 cut(s)	459, 1006, 1040, 1147, 1741, 2074, 2235
BstXI	CCANNNNNNTGG	1 cut(s)	922
BstYI	RGATCY	7 cut(s)	459, 1006, 1040, 1147, 1741, 2074, 2235
BsuRI	GGCC	7 cut(s)	33, 58, 376, 387, 494, 706, 954
BtgI	CCRYGG	1 cut(s)	594
BtgZI	GCGATG	3 cut(s)	654, 821, 1417
BtrI	CACGTC	1 cut(s)	490
BtsCI	GGATG	7 cut(s)	90, 511, 899, 1688, 1696, 1763, 1969
BtsIMutI	CAGTG	2 cut(s)	906, 1368
BtuMI	TCGCGA	1 cut(s)	454
BveI	ACCTGC	2 cut(s)	173, 949
Cac8I	GCNNGC	5 cut(s)	113, 202, 513, 1129, 1638
CaiI	CAGNNNCTG	1 cut(s)	770
CciI	TCATGA	4 cut(s)	607, 1173, 1902, 2083
CfoI	GCGC	6 cut(s)	182, 202, 204, 326, 603, 2226
Cfr13I	GGNCC	7 cut(s)	42, 375, 938, 953, 1325, 1940, 2034
Cfr9I	CCCGGG	1 cut(s)	501
CseI	GACGC	2 cut(s)	91, 193
Csp6I	GTAC	3 cut(s)	334, 1389, 2410
CviQI	GTAC	3 cut(s)	334, 1389, 2410
DdeI	CTNAG	5 cut(s)	98, 771, 1371, 1653, 1812
DinI	GGCGCC	1 cut(s)	181
EaeI	YGGCCR	2 cut(s)	56, 492
Eam1104I	CTCTTC	4 cut(s)	314, 1239, 1344, 2265
EarI	CTCTTC	4 cut(s)	314, 1239, 1344, 2265
Ecl136II	GAGCTC	2 cut(s)	136, 307
Eco130I	CCWWGG	3 cut(s)	594, 707, 1321
Eco147I	AGGCCT	2 cut(s)	33, 706
Eco24I	GRGCYC	3 cut(s)	138, 309, 1321
Eco31I	GGTCTC	1 cut(s)	1815
Eco47I	GGWCC	5 cut(s)	42, 938, 1325, 1940, 2034
Eco53kI	GAGCTC	2 cut(s)	136, 307
Eco57I	CTGAAG	3 cut(s)	542, 659, 2215
Eco88I	CYCGRG	4 cut(s)	349, 501, 614, 1668
Eco91I	GGTNACC	1 cut(s)	1571
EcoICRI	GAGCTC	2 cut(s)	136, 307
EcoO109I	RGGNCCY	1 cut(s)	1325
EcoO65I	GGTNACC	1 cut(s)	1571
EcoRI	GAATTC	1 cut(s)	2299
EcoRII	CCWGG	7 cut(s)	44, 53, 829, 841, 955, 1631, 1959
EcoT14I	CCWWGG	3 cut(s)	594, 707, 1321
EcoT22I	ATGCAT	2 cut(s)	1087, 1433
EcoT38I	GRGCYC	3 cut(s)	138, 309, 1321
EgeI	GGCGCC	1 cut(s)	181
EheI	GGCGCC	1 cut(s)	181
ErhI	CCWWGG	3 cut(s)	594, 707, 1321
FalI	AAGNNNNNCTT	4 cut(s)	242, 274, 1458, 1490
FaqI	GGGAC	1 cut(s)	339
FauI	CCCGC	5 cut(s)	227, 307, 364, 370, 475
FbaI	TGATCA	1 cut(s)	1120
FblI	GTMKAC	3 cut(s)	207, 486, 969
FokI	GGATG	7 cut(s)	77, 518, 906, 1695, 1703, 1750, 1976
FriOI	GRGCYC	3 cut(s)	138, 309, 1321
FspBI	CTAG	4 cut(s)	708, 1181, 1322, 2205
GlaI	GCGC	6 cut(s)	181, 201, 203, 325, 602, 2225
GsaI	CCCAGC	1 cut(s)	1599
GsuI	CTGGAG	4 cut(s)	566, 616, 898, 1965
HaeII	RGCGCY	3 cut(s)	183, 327, 2227
HaeIII	GGCC	7 cut(s)	33, 58, 376, 387, 494, 706, 954
HapII	CCGG	4 cut(s)	438, 502, 557, 1534
HgaI	GACGC	2 cut(s)	91, 193
HhaI	GCGC	6 cut(s)	182, 202, 204, 326, 603, 2226
Hin1I	GRCGYC	1 cut(s)	180
Hin6I	GCGC	6 cut(s)	180, 200, 202, 324, 601, 2224
HinP1I	GCGC	6 cut(s)	180, 200, 202, 324, 601, 2224
HincII	GTYRAC	6 cut(s)	208, 487, 852, 1077, 1351, 2256
HindII	GTYRAC	6 cut(s)	208, 487, 852, 1077, 1351, 2256
HindIII	AAGCTT	1 cut(s)	2057
HpaI	GTTAAC	3 cut(s)	1077, 1351, 2256
HpaII	CCGG	4 cut(s)	438, 502, 557, 1534
HphI	GGTGA	8 cut(s)	154, 913, 992, 1391, 1555, 1572, 1637, 2377
Hpy99I	CGWCG	6 cut(s)	209, 332, 427, 430, 488, 491
HpyAV	CCTTC	5 cut(s)	23, 268, 614, 840, 884
HpyCH4III	ACNGT	6 cut(s)	949, 1497, 1577, 1877, 1940, 2147
HpyCH4IV	ACGT	4 cut(s)	489, 584, 1697, 2408
HpyF3I	CTNAG	5 cut(s)	98, 771, 1371, 1653, 1812
HpySE526I	ACGT	4 cut(s)	489, 584, 1697, 2408
Hsp92I	GRCGYC	1 cut(s)	180
HspAI	GCGC	6 cut(s)	180, 200, 202, 324, 601, 2224
KasI	GGCGCC	1 cut(s)	179
Kpn2I	TCCGGA	1 cut(s)	1533
KpnI	GGTACC	1 cut(s)	1392
Ksp22I	TGATCA	1 cut(s)	1120
KspAI	GTTAAC	3 cut(s)	1077, 1351, 2256
LguI	GCTCTTC	2 cut(s)	314, 2265
MaeI	CTAG	4 cut(s)	708, 1181, 1322, 2205
MaeII	ACGT	4 cut(s)	489, 584, 1697, 2408
MaeIII	GTNAC	7 cut(s)	160, 580, 1065, 1288, 1543, 1571, 2174
MauBI	CGCGCGCG	1 cut(s)	200
MbiI	CCGCTC	1 cut(s)	83
MfeI	CAATTG	2 cut(s)	370, 798
MflI	RGATCY	7 cut(s)	459, 1006, 1040, 1147, 1741, 2074, 2235
MhlI	GDGCHC	6 cut(s)	113, 138, 309, 632, 908, 1321
MlsI	TGGCCA	1 cut(s)	494
MluNI	TGGCCA	1 cut(s)	494
Mly113I	GGCGCC	1 cut(s)	180
MlyI	GAGTC	5 cut(s)	361, 645, 965, 1196, 1205
MmeI	TCCRAC	5 cut(s)	300, 423, 487, 1791, 2362
Mox20I	TGGCCA	1 cut(s)	494
Mph1103I	ATGCAT	2 cut(s)	1087, 1433
MroI	TCCGGA	1 cut(s)	1533
MroXI	GAANNNNTTC	4 cut(s)	257, 1205, 2274, 2333
MscI	TGGCCA	1 cut(s)	494
MseI	TTAA	8 cut(s)	699, 1076, 1143, 1305, 1350, 1830, 2169, 2255
Msp20I	TGGCCA	1 cut(s)	494
MspA1I	CMGCKG	2 cut(s)	118, 1616
MspCI	CTTAAG	1 cut(s)	1142
MspI	CCGG	4 cut(s)	438, 502, 557, 1534
MunI	CAATTG	2 cut(s)	370, 798
Mva1269I	GAATGC	1 cut(s)	1484
MvaI	CCWGG	7 cut(s)	46, 55, 831, 843, 957, 1633, 1961
MvnI	CGCG	5 cut(s)	200, 202, 204, 454, 470
NarI	GGCGCC	1 cut(s)	180
NciI	CCSGG	3 cut(s)	439, 502, 503
NcoI	CCATGG	1 cut(s)	594
NlaIV	GGNNCC	9 cut(s)	181, 461, 913, 1149, 1318, 1390, 2035, 2076, 2237
NmeAIII	GCCGAG	3 cut(s)	157, 328, 1460
NmuCI	GTSAC	6 cut(s)	160, 580, 1065, 1288, 1543, 2174
NruI	TCGCGA	1 cut(s)	454
NsiI	ATGCAT	2 cut(s)	1087, 1433
NspI	RCATGY	2 cut(s)	1431, 2310
PaeR7I	CTCGAG	2 cut(s)	614, 1668
PagI	TCATGA	4 cut(s)	607, 1173, 1902, 2083
PaqCI	CACCTGC	1 cut(s)	173
PauI	GCGCGC	1 cut(s)	200
PceI	AGGCCT	2 cut(s)	33, 706
PciI	ACATGT	1 cut(s)	2306
PciSI	GCTCTTC	2 cut(s)	314, 2265
PcsI	WCGNNNNNNNCGW	4 cut(s)	204, 410, 419, 621
PctI	GAATGC	1 cut(s)	1484
PdmI	GAANNNNTTC	4 cut(s)	257, 1205, 2274, 2333
PfeI	GAWTC	7 cut(s)	449, 590, 679, 918, 1201, 1530, 2003
Pfl23II	CGTACG	1 cut(s)	2409
PflFI	GACNNNGTC	1 cut(s)	158
PfoI	TCCNGGA	2 cut(s)	44, 1959
PleI	GAGTC	5 cut(s)	360, 644, 965, 1195, 1204
PluTI	GGCGCC	1 cut(s)	183
PpsI	GAGTC	5 cut(s)	360, 644, 965, 1195, 1204
PpuMI	RGGWCCY	1 cut(s)	1325
PscI	ACATGT	1 cut(s)	2306
PshAI	GACNNNNGTC	1 cut(s)	1063
PshBI	ATTAAT	1 cut(s)	699
Psp124BI	GAGCTC	2 cut(s)	138, 309
Psp5II	RGGWCCY	1 cut(s)	1325
Psp6I	CCWGG	7 cut(s)	44, 53, 829, 841, 955, 1631, 1959
PspEI	GGTNACC	1 cut(s)	1571
PspFI	CCCAGC	1 cut(s)	1595
PspGI	CCWGG	7 cut(s)	44, 53, 829, 841, 955, 1631, 1959
PspLI	CGTACG	1 cut(s)	2409
PspN4I	GGNNCC	9 cut(s)	181, 461, 913, 1149, 1318, 1390, 2035, 2076, 2237
PspPI	GGNCC	7 cut(s)	42, 375, 938, 953, 1325, 1940, 2034
PspPPI	RGGWCCY	1 cut(s)	1325
PstI	CTGCAG	1 cut(s)	1138
PstNI	CAGNNNCTG	1 cut(s)	770
PsuI	RGATCY	7 cut(s)	459, 1006, 1040, 1147, 1741, 2074, 2235
PsyI	GACNNNGTC	1 cut(s)	158
PteI	GCGCGC	1 cut(s)	200
PvuII	CAGCTG	1 cut(s)	1616
RruI	TCGCGA	1 cut(s)	454
RsaI	GTAC	3 cut(s)	335, 1390, 2411
RsaNI	GTAC	3 cut(s)	334, 1389, 2410
SacI	GAGCTC	2 cut(s)	138, 309
SalI	GTCGAC	2 cut(s)	206, 485
SapI	GCTCTTC	2 cut(s)	314, 2265
SaqAI	TTAA	8 cut(s)	699, 1076, 1143, 1305, 1350, 1830, 2169, 2255
Sau96I	GGNCC	7 cut(s)	42, 375, 938, 953, 1325, 1940, 2034
SchI	GAGTC	5 cut(s)	361, 645, 965, 1196, 1205
SduI	GDGCHC	6 cut(s)	113, 138, 309, 632, 908, 1321
SfcI	CTRYAG	4 cut(s)	948, 1033, 1054, 1134
SfoI	GGCGCC	1 cut(s)	181
Sfr274I	CTCGAG	2 cut(s)	614, 1668
SgrDI	CGTCGACG	1 cut(s)	485
SinI	GGWCC	5 cut(s)	42, 938, 1325, 1940, 2034
SlaI	CTCGAG	2 cut(s)	614, 1668
SmaI	CCCGGG	1 cut(s)	503
SmlI	CTYRAG	4 cut(s)	614, 1142, 1668, 1801
SmoI	CTYRAG	4 cut(s)	614, 1142, 1668, 1801
SseBI	AGGCCT	2 cut(s)	33, 706
SspDI	GGCGCC	1 cut(s)	179
SspI	AATATT	1 cut(s)	1928
SspMI	CTAG	4 cut(s)	708, 1181, 1322, 2205
SstI	GAGCTC	2 cut(s)	138, 309
StuI	AGGCCT	2 cut(s)	33, 706
StyI	CCWWGG	3 cut(s)	594, 707, 1321
TaaI	ACNGT	6 cut(s)	949, 1497, 1577, 1877, 1940, 2147
TaiI	ACGT	4 cut(s)	492, 587, 1700, 2411
TaqI	TCGA	9 cut(s)	207, 330, 413, 486, 615, 1098, 1199, 1602, 1669
TauI	GCSGC	7 cut(s)	83, 118, 180, 387, 518, 875, 1225
TfiI	GAWTC	7 cut(s)	449, 590, 679, 918, 1201, 1530, 2003
Tru1I	TTAA	8 cut(s)	699, 1076, 1143, 1305, 1350, 1830, 2169, 2255
Tru9I	TTAA	8 cut(s)	699, 1076, 1143, 1305, 1350, 1830, 2169, 2255
TscAI	CASTG	2 cut(s)	913, 1375
TseFI	GTSAC	6 cut(s)	160, 580, 1065, 1288, 1543, 2174
Tsp45I	GTSAC	6 cut(s)	160, 580, 1065, 1288, 1543, 2174
TspDTI	ATGAA	6 cut(s)	596, 1162, 1257, 1489, 1780, 2003
TspGWI	ACGGA	1 cut(s)	666
TspMI	CCCGGG	1 cut(s)	501
TspRI	CASTG	2 cut(s)	913, 1375
Tth111I	GACNNNGTC	1 cut(s)	158
Vha464I	CTTAAG	1 cut(s)	1142
VneI	GTGCAC	1 cut(s)	109
VpaK11BI	GGWCC	5 cut(s)	42, 938, 1325, 1940, 2034
VspI	ATTAAT	1 cut(s)	699
XapI	RAATTY	3 cut(s)	1718, 2089, 2299
XceI	RCATGY	2 cut(s)	1431, 2310
XhoI	CTCGAG	2 cut(s)	614, 1668
XmaI	CCCGGG	1 cut(s)	501
XmaJI	CCTAGG	2 cut(s)	707, 1321
XmiI	GTMKAC	3 cut(s)	207, 486, 969
XmnI	GAANNNNTTC	4 cut(s)	257, 1205, 2274, 2333
XspI	CTAG	4 cut(s)	708, 1181, 1322, 2205
Zsp2I	ATGCAT	2 cut(s)	1087, 1433

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.