Rorug05G0430200

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0023906
Physical Location & Seq
Forward (+)
59815890 .. 59816938
1049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0430200.1

Sequence Viewer

Length: 1002 bp
ATGGCGGCGGAGGAGGGCGGCGTCTGTAAGCGAGCTCACACCGAGTTCGACGACGGCGAAATCATTTCAGAGATTCTGGCGAGGCTACCGGTCAAATGTGTGATGCGATTCCGGTGCGTATCCAAGTGGTGGAATTCTCTGATCTCCGATCCTTACTTCACTAAGAATCACTTGAGGTACGCAGAGATGGGCATCACCGAGAACAGCTCCAGGCTCCTTTATTCGATGGACCCTCCGCAATCCCTAGACCACGAAGCATTGAAGGATTTGAAGGATCATAGTGATGTTTATTTTGCCAACAGAGAGCTTGGTCTTCCGGTAATATTGGCGGGCTACTCCAATTGCTATAAAATTGTTGTTGGTAGTTGCAATGGGTTAATATGTATTTTTGTTGAAGGAGTCGACTTTATCTTATGGAATCCATGTACTGGATACTCCAAGTTGTTACCAAGAACTCCATTGCCCGTTTTTAATCCACGTGGTTGTTGCAATAGTGGGTTTGGTTATGATTCTGCTAATGATGATTACAAGGTATTAGTGGGGACTAAAGAGATGGGTGAAGAAAATTCCATTCAGGTTTTCACTCTGAAAACGAGTTCATGGAAGGTGCTCCCCATTACCAATGCTCTTAACATCTCAGGTCGGGGGTTGTTTTTTAATGGAGCTTTGCACTGGTTGAATCGCCAAACAATTTTGTCTTTTAATTTGGTGGAGGACAAGTTTCATCAGATGATTCCATTACCCTATGATGATGATGATGATGATGCTTACTTTCGAGGTATGATGATCCATGGAAATTGTCTTTGTGTGTCTGATGTTGGCCGGGATGGTCTTAACGCAATATGGTTGATGAAGGAGTATGGGGTCAAGCAATCTTGGACTCAAGTTATAAAGTTTCCTACAGCGTACAGCCACGCCTGCTTGTGGCCTATTTGTATTCTAGAGAGAGGAGAACTTTTGGTTAACAATGGGCGTACAGGTTTCTTGGTGTTATATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

333

Amino Acids

37.67

Weight (kDa)

5.64

Isoelectric Point (pI)

30.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 20 - 55 1.7e-06 F-box domain
FBA_3 PF08268 115 - 299 3.8e-19 F-box associated beta propeller domain
FBA_1 PF07734 120 - 298 1e-23 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023906)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34015
prunus_persica Prupe.4G019300_v2.0.a1
rosa_rugosa Rorug05G0430200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 890
AccB7I CCANNNNNTGG 2 cut(s) 129, 428
AccI GTMKAC 1 cut(s) 402
AciI CCGC 5 cut(s) 5, 8, 18, 236, 329
AclWI GGATC 3 cut(s) 143, 282, 781
AcoI YGGCCR 1 cut(s) 820
AcsI RAATTY 2 cut(s) 133, 565
AcvI CACGTG 1 cut(s) 479
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 4 cut(s) 179, 427, 908, 976
AfiI CCNNNNNNNGG 3 cut(s) 129, 428, 924
AgeI ACCGGT 1 cut(s) 88
AgsI TTSAA 4 cut(s) 262, 271, 395, 679
AjnI CCWGG 1 cut(s) 209
AluBI AGCT 4 cut(s) 35, 207, 307, 665
AluI AGCT 4 cut(s) 35, 207, 307, 665
Alw21I GWGCWC 2 cut(s) 37, 612
AlwI GGATC 3 cut(s) 143, 282, 781
AoxI GGCC 2 cut(s) 820, 926
ApoI RAATTY 2 cut(s) 133, 565
AsiGI ACCGGT 1 cut(s) 88
AspS9I GGNCC 1 cut(s) 229
AsuC2I CCSGG 1 cut(s) 824
AsuHPI GGTGA 2 cut(s) 187, 569
AvaII GGWCC 1 cut(s) 229
BanII GRGCYC 1 cut(s) 37
BbrPI CACGTG 1 cut(s) 479
BbsI GAAGAC 1 cut(s) 305
Bbv12I GWGCWC 2 cut(s) 37, 612
BccI CCATC 4 cut(s) 181, 220, 547, 821
BceAI ACGGC 1 cut(s) 70
BcgI CGANNNNNNTGC 2 cut(s) 96, 130
BciT130I CCWGG 1 cut(s) 211
BciVI GTATCC 2 cut(s) 130, 425
BcnI CCSGG 1 cut(s) 824
BfaI CTAG 2 cut(s) 245, 941
BfmI CTRYAG 1 cut(s) 900
BfuI GTATCC 2 cut(s) 130, 425
BisI GCNGC 2 cut(s) 6, 19
BlsI GCNGC 2 cut(s) 7, 20
Bme1390I CCNGG 2 cut(s) 211, 824
Bme18I GGWCC 1 cut(s) 229
BmgT120I GGNCC 1 cut(s) 229
BmiI GGNNCC 2 cut(s) 215, 231
BmrFI CCNGG 2 cut(s) 211, 824
BmsI GCATC 3 cut(s) 93, 201, 754
BpiI GAAGAC 1 cut(s) 305
BplI GAGNNNNNCTC 2 cut(s) 191, 223
BpmI CTGGAG 1 cut(s) 193
BpuEI CTTGAG 2 cut(s) 193, 867
BpuMI CCSGG 1 cut(s) 824
BsaAI YACGTR 1 cut(s) 479
BsaBI GATNNNNATC 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 1 cut(s) 790
BsaWI WCCGGW 3 cut(s) 88, 111, 316
BsaXI ACNNNNNCTCC 2 cut(s) 704, 734
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 428, 924
Bse118I RCCGGY 1 cut(s) 88
Bse1I ACTGG 2 cut(s) 433, 677
Bse3DI GCAATG 2 cut(s) 376, 458
Bse8I GATNNNNATC 1 cut(s) 191
BseBI CCWGG 1 cut(s) 211
BseDI CCNNGG 1 cut(s) 790
BseGI GGATG 1 cut(s) 832
BseJI GATNNNNATC 1 cut(s) 191
BseLI CCNNNNNNNGG 3 cut(s) 129, 428, 924
BseMI GCAATG 2 cut(s) 376, 458
BseMII CTCAG 1 cut(s) 651
BseNI ACTGG 2 cut(s) 433, 677
BseRI GAGGAG 2 cut(s) 26, 963
BshFI GGCC 2 cut(s) 822, 928
BshTI ACCGGT 1 cut(s) 88
BsiHKAI GWGCWC 2 cut(s) 37, 612
BsiSI CCGG 4 cut(s) 89, 112, 317, 823
BslFI GGGAC 1 cut(s) 556
BslI CCNNNNNNNGG 3 cut(s) 129, 428, 924
BsmFI GGGAC 1 cut(s) 556
BsnI GGCC 2 cut(s) 822, 928
Bsp1286I GDGCHC 2 cut(s) 37, 612
Bsp143I GATC 4 cut(s) 141, 148, 274, 786
Bsp19I CCATGG 1 cut(s) 790
BspACI CCGC 5 cut(s) 5, 8, 18, 236, 329
BspANI GGCC 2 cut(s) 822, 928
BspCNI CTCAG 1 cut(s) 650
BspLI GGNNCC 2 cut(s) 215, 231
BspPI GGATC 3 cut(s) 143, 282, 781
BsrDI GCAATG 2 cut(s) 376, 458
BsrFI RCCGGY 1 cut(s) 88
BsrI ACTGG 2 cut(s) 433, 677
BssAI RCCGGY 1 cut(s) 88
BssECI CCNNGG 1 cut(s) 790
BssMI GATC 4 cut(s) 141, 148, 274, 786
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 1 cut(s) 790
Bst2UI CCWGG 1 cut(s) 211
BstACI GRCGYC 1 cut(s) 21
BstBAI YACGTR 1 cut(s) 479
BstC8I GCNNGC 3 cut(s) 33, 331, 919
BstDEI CTNAG 2 cut(s) 162, 637
BstDSI CCRYGG 1 cut(s) 790
BstF5I GGATG 1 cut(s) 832
BstKTI GATC 4 cut(s) 144, 151, 277, 789
BstMBI GATC 4 cut(s) 141, 148, 274, 786
BstMWI GCNNNNNNNGC 1 cut(s) 918
BstNI CCWGG 1 cut(s) 211
BstSCI CCNGG 2 cut(s) 209, 822
BstSFI CTRYAG 1 cut(s) 900
BstV2I GAAGAC 1 cut(s) 305
BsuI GTATCC 2 cut(s) 130, 425
BsuRI GGCC 2 cut(s) 822, 928
BtgI CCRYGG 1 cut(s) 790
BtsCI GGATG 1 cut(s) 832
BtsIMutI CAGTG 1 cut(s) 670
Cac8I GCNNGC 3 cut(s) 33, 331, 919
Cfr10I RCCGGY 1 cut(s) 88
Cfr13I GGNCC 1 cut(s) 229
CseI GACGC 1 cut(s) 10
Csp6I GTAC 4 cut(s) 178, 426, 907, 975
CspAI ACCGGT 1 cut(s) 88
CviAII CATG 3 cut(s) 423, 600, 791
CviQI GTAC 4 cut(s) 178, 426, 907, 975
DdeI CTNAG 2 cut(s) 162, 637
DpnI GATC 4 cut(s) 143, 150, 276, 788
DpnII GATC 4 cut(s) 141, 148, 274, 786
EaeI YGGCCR 1 cut(s) 820
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 790
Eco24I GRGCYC 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 229
Eco53kI GAGCTC 1 cut(s) 35
Eco72I CACGTG 1 cut(s) 479
EcoICRI GAGCTC 1 cut(s) 35
EcoRI GAATTC 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 209
EcoT14I CCWWGG 1 cut(s) 790
EcoT38I GRGCYC 1 cut(s) 37
ErhI CCWWGG 1 cut(s) 790
FaeI CATG 3 cut(s) 426, 603, 794
FalI AAGNNNNNCTT 2 cut(s) 155, 187
FaqI GGGAC 1 cut(s) 556
FatI CATG 3 cut(s) 422, 599, 790
FauI CCCGC 1 cut(s) 322
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 2 cut(s) 6, 19
FokI GGATG 1 cut(s) 839
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 2 cut(s) 6, 19
FspBI CTAG 2 cut(s) 245, 941
GluI GCNGC 2 cut(s) 6, 19
GsuI CTGGAG 1 cut(s) 193
HaeIII GGCC 2 cut(s) 822, 928
HapII CCGG 4 cut(s) 89, 112, 317, 823
HgaI GACGC 1 cut(s) 10
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 3 cut(s) 426, 603, 794
HincII GTYRAC 2 cut(s) 403, 964
HindII GTYRAC 2 cut(s) 403, 964
HinfI GANTC 9 cut(s) 73, 108, 166, 399, 418, 509, 679, 733, 880
HpaI GTTAAC 1 cut(s) 964
HpaII CCGG 4 cut(s) 89, 112, 317, 823
HphI GGTGA 2 cut(s) 187, 569
Hpy166II GTNNAC 2 cut(s) 403, 964
Hpy188I TCNGA 6 cut(s) 70, 141, 148, 588, 729, 814
Hpy188III TCNNGA 1 cut(s) 941
Hpy8I GTNNAC 2 cut(s) 403, 964
Hpy99I CGWCG 2 cut(s) 53, 56
HpyAV CCTTC 5 cut(s) 256, 265, 389, 598, 847
HpyCH4IV ACGT 1 cut(s) 478
HpyCH4V TGCA 3 cut(s) 369, 489, 670
HpyF10VI GCNNNNNNNGC 1 cut(s) 918
HpyF3I CTNAG 2 cut(s) 162, 637
HpySE526I ACGT 1 cut(s) 478
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 3 cut(s) 426, 603, 794
KspAI GTTAAC 1 cut(s) 964
Kzo9I GATC 4 cut(s) 141, 148, 274, 786
LmnI GCTCC 4 cut(s) 212, 219, 615, 662
LweI GCATC 3 cut(s) 93, 201, 754
MaeI CTAG 2 cut(s) 245, 941
MaeII ACGT 1 cut(s) 478
MaeIII GTNAC 1 cut(s) 444
MalI GATC 4 cut(s) 143, 150, 276, 788
MboI GATC 4 cut(s) 141, 148, 274, 786
MboII GAAGA 2 cut(s) 305, 572
MfeI CAATTG 1 cut(s) 340
MhlI GDGCHC 2 cut(s) 37, 612
MluCI AATT 7 cut(s) 133, 340, 351, 565, 690, 703, 796
MlyI GAGTC 2 cut(s) 408, 874
MnlI CCTC 8 cut(s) 4, 7, 75, 168, 243, 706, 770, 941
MseI TTAA 7 cut(s) 377, 471, 630, 657, 702, 834, 963
MslI CAYNNNNRTG 1 cut(s) 282
MspI CCGG 4 cut(s) 89, 112, 317, 823
MspR9I CCNGG 2 cut(s) 211, 824
MunI CAATTG 1 cut(s) 340
MvaI CCWGG 1 cut(s) 211
MwoI GCNNNNNNNGC 1 cut(s) 918
NciI CCSGG 1 cut(s) 824
NcoI CCATGG 1 cut(s) 790
NdeII GATC 4 cut(s) 141, 148, 274, 786
NlaIII CATG 3 cut(s) 426, 603, 794
NlaIV GGNNCC 2 cut(s) 215, 231
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PfeI GAWTC 7 cut(s) 73, 108, 166, 418, 509, 679, 733
PflMI CCANNNNNTGG 2 cut(s) 129, 428
PinAI ACCGGT 1 cut(s) 88
PkrI GCNGC 2 cut(s) 7, 20
PleI GAGTC 2 cut(s) 407, 874
PmaCI CACGTG 1 cut(s) 479
PmlI CACGTG 1 cut(s) 479
PpsI GAGTC 2 cut(s) 407, 874
Ppu21I YACGTR 1 cut(s) 479
PsiI TTATAA 1 cut(s) 890
Psp124BI GAGCTC 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 209
PspCI CACGTG 1 cut(s) 479
PspGI CCWGG 1 cut(s) 209
PspN4I GGNNCC 2 cut(s) 215, 231
PspPI GGNCC 1 cut(s) 229
RsaI GTAC 4 cut(s) 179, 427, 908, 976
RsaNI GTAC 4 cut(s) 178, 426, 907, 975
RseI CAYNNNNRTG 1 cut(s) 282
SacI GAGCTC 1 cut(s) 37
SalI GTCGAC 1 cut(s) 401
SaqAI TTAA 7 cut(s) 377, 471, 630, 657, 702, 834, 963
SatI GCNGC 2 cut(s) 6, 19
Sau3AI GATC 4 cut(s) 141, 148, 274, 786
Sau96I GGNCC 1 cut(s) 229
SchI GAGTC 2 cut(s) 408, 874
ScrFI CCNGG 2 cut(s) 211, 824
SduI GDGCHC 2 cut(s) 37, 612
SfaNI GCATC 3 cut(s) 93, 201, 754
SfcI CTRYAG 1 cut(s) 900
SinI GGWCC 1 cut(s) 229
SmiMI CAYNNNNRTG 1 cut(s) 282
SmlI CTYRAG 2 cut(s) 172, 882
SmoI CTYRAG 2 cut(s) 172, 882
Sse9I AATT 7 cut(s) 133, 340, 351, 565, 690, 703, 796
SsiI CCGC 5 cut(s) 5, 8, 18, 236, 329
SspI AATATT 1 cut(s) 324
SspMI CTAG 2 cut(s) 245, 941
SstI GAGCTC 1 cut(s) 37
StyD4I CCNGG 2 cut(s) 209, 822
StyI CCWWGG 1 cut(s) 790
TaiI ACGT 1 cut(s) 481
TaqI TCGA 4 cut(s) 48, 224, 402, 775
TasI AATT 7 cut(s) 133, 340, 351, 565, 690, 703, 796
TatI WGTACW 1 cut(s) 425
TauI GCSGC 2 cut(s) 8, 21
TfiI GAWTC 7 cut(s) 73, 108, 166, 418, 509, 679, 733
Tru1I TTAA 7 cut(s) 377, 471, 630, 657, 702, 834, 963
Tru9I TTAA 7 cut(s) 377, 471, 630, 657, 702, 834, 963
TscAI CASTG 1 cut(s) 677
TspDTI ATGAA 3 cut(s) 588, 713, 866
TspRI CASTG 1 cut(s) 677
Van91I CCANNNNNTGG 2 cut(s) 129, 428
VpaK11BI GGWCC 1 cut(s) 229
XapI RAATTY 2 cut(s) 133, 565
XbaI TCTAGA 1 cut(s) 940
XmiI GTMKAC 1 cut(s) 402
XspI CTAG 2 cut(s) 245, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.