Rorug05G0549600

Receptor-like protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0024329
Physical Location & Seq
Forward (+)
73796947 .. 73798359
1413 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0549600.1

Sequence Viewer

Length: 1413 bp
ATGGTGAAGAGCACCGAACGCGTTTGCTACTATGAGACCTTGGGCCTCGAACCCGTCTGCTCGATGGATGATATCAGACATGCTTACCATGAACTGTCCAAAATATACCACCCGGACAAATACAACTCCAGTTGCGGTGTGCCAAAAGATGAAGCCTCCGCCAAATTTCTAGCAATCAAAGACGCCTACGACATCCTCAAAGAACCCCATGAGCGAGCCTTCTACGACTCCTACCTCCAGACCTACGCTGTGCCAGAGGCTTTCAACCCCGACCTCGAAGCCGCCTTCGACAACATCGACTTCAACGGCTACTCCGACTCCGGCCGCGGCTTCTATAAGGTCTACTCCGAAGTTTTTGAAAGGATTTACGCCAACGAGCGTGCTTTTCAAGAAAGGAATGACCTGCCGTGGGGTTCTGTTCACAGCCCTCCGCGCATGGGAAATCTGGACAGCCCGTATCCTGAGGTTGTTCAGTTTTATAACTACTGGCTCAACTTTAGTTCCATTATGGATTTTTGCTGGGAAGAACCTCATGATGAGTACGATCTGAGTCAGGTCTCCCGCAGAAGAGTGAAGCTGTGGTCCCGCCAAAACATGAAAGCTAGGAAGAAGGCCAAGAAGGAGTACAACAACAAGGTGCGGAGTTTGGCACAAAATTCCAAGAGGCTTGACAGGAGGGTTATGCAGATGACGGCGAAGAGGGAGGAGGAGAGAAAGAGGGAGATGGAAGAGGAGAACGAGAGGAGGAAGCGGTTAAAGAAAGAGAAGTTGGAGAAAGCGGCTAGGGAGTACGAGGAGCCGGAGTGGACCAAAGTGGTTGAGAGGAGGAGAAAATATGGTGATCCTGAGGAGGAGAAGATAAAGGAGATAGAGGAATGGGAGTGTGTTGTTTGTAGGAAGGGATTTAGGAGTGAGAAGCAGTGCAGGAATCACGAGCAGTCGAAGAAGCACCTTCGTAGGGTTTCAGAGTTAATAGAGTTGCAATCTAACCATTGTGAAATTGTTGTTGAGTCGATGGAGGACAAGAGGGATTACGATGAGCTTGATAAAGAAGAGGTTTCGGTGAAAGCAAAAGGGGATGAAGTGGCTGGAGAGAGTGATGAGTTTTCTGATTGTGTCAGTGACAATAGGTGGAAAAACTTTAGTGAAGCAGTTAAATATGATGAGGAGGAGGAGGAGGAGGTTGTGGATGAGATGGGTGTTCTTCTAGCCATGGTGGCAAAGCAAAAGACTATGGAAAAGGTTGGTAAACCAGTTTTTGAAGCAATGGCTGAGTATATGGCGACCGGTAGTCATGTGGATAACGATGCCGATGAGGTAGCCATAAAACATGATCAGCTAAAGCAAATGAATAATAATGGAGGAGACAGGAAACCGAAGAGAAGGAGGAGGGTGAAGACTGAAGAGTACTAA

Protein Analysis

470

Amino Acids

55.86

Weight (kDa)

5.49

Isoelectric Point (pI)

66.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 10 - 76 1.6e-15 DnaJ domain
ZUO1-like_ZHD PF21884 111 - 227 8.5e-17 Zuotin-like, zuotin homology domain
zf-C2H2_jaz PF12171 293 - 317 5.9e-07 Zinc-finger double-stranded RNA-binding
zf-met PF12874 293 - 317 1.6e-06 Zinc-finger of C2H2 type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024329)

Species Orthologous Gene IDs
malus_domestica MD03G1131400.v1.1
rosa_rugosa Rorug05G0468600 Rorug05G0549600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 480
Acc36I ACCTGC 1 cut(s) 411
AccI GTMKAC 1 cut(s) 342
AccII CGCG 3 cut(s) 21, 327, 433
AclWI GGATC 1 cut(s) 836
AcoI YGGCCR 1 cut(s) 322
AcsI RAATTY 2 cut(s) 164, 655
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 4 cut(s) 542, 626, 791, 1409
AfiI CCNNNNNNNGG 3 cut(s) 409, 437, 958
AflIII ACRYGT 1 cut(s) 19
AgeI ACCGGT 1 cut(s) 1286
AgsI TTSAA 5 cut(s) 265, 304, 359, 389, 1262
AhdI GACNNNNNGTC 1 cut(s) 1290
AjuI GAANNNNNNNTTGG 2 cut(s) 752, 784
AluBI AGCT 4 cut(s) 577, 602, 1042, 1339
AluI AGCT 4 cut(s) 577, 602, 1042, 1339
Alw21I GWGCWC 1 cut(s) 14
Alw26I GTCTC 3 cut(s) 29, 562, 1359
AlwI GGATC 1 cut(s) 836
AoxI GGCC 3 cut(s) 43, 322, 612
ApoI RAATTY 2 cut(s) 164, 655
AsiGI ACCGGT 1 cut(s) 1286
AspLEI GCGC 1 cut(s) 435
AspS9I GGNCC 3 cut(s) 43, 582, 807
AsuC2I CCSGG 1 cut(s) 113
AsuHPI GGTGA 4 cut(s) 16, 851, 1075, 1405
AvaII GGWCC 2 cut(s) 582, 807
AxyI CCTNAGG 2 cut(s) 462, 846
BauI CACGAG 1 cut(s) 932
BbsI GAAGAC 1 cut(s) 1403
Bbv12I GWGCWC 1 cut(s) 14
BccI CCATC 4 cut(s) 58, 718, 1009, 1189
BceAI ACGGC 3 cut(s) 322, 391, 708
BciVI GTATCC 1 cut(s) 468
BclI TGATCA 1 cut(s) 1333
BcnI CCSGG 1 cut(s) 113
BcoDI GTCTC 3 cut(s) 29, 562, 1359
BfaI CTAG 4 cut(s) 170, 603, 783, 1208
BfuAI ACCTGC 1 cut(s) 411
BfuI GTATCC 1 cut(s) 468
BglI GCCNNNNNGGC 1 cut(s) 1217
BisI GCNGC 4 cut(s) 282, 325, 328, 780
BlsI GCNGC 4 cut(s) 283, 326, 329, 781
BmcAI AGTACT 1 cut(s) 1409
Bme1390I CCNGG 1 cut(s) 113
Bme18I GGWCC 2 cut(s) 582, 807
BmeRI GACNNNNNGTC 1 cut(s) 1290
BmgT120I GGNCC 3 cut(s) 43, 582, 807
BmiI GGNNCC 2 cut(s) 584, 798
BmrFI CCNGG 1 cut(s) 113
BmsI GCATC 1 cut(s) 1297
BpiI GAAGAC 1 cut(s) 1403
BpmI CTGGAG 3 cut(s) 112, 221, 1110
BpuMI CCSGG 1 cut(s) 113
BsaHI GRCGYC 1 cut(s) 183
BsaI GGTCTC 2 cut(s) 29, 562
BsaJI CCNNGG 4 cut(s) 39, 325, 407, 1212
BsaWI WCCGGW 1 cut(s) 1286
BsaXI ACNNNNNCTCC 6 cut(s) 296, 302, 326, 332, 788, 818
Bsc4I CCNNNNNNNGG 3 cut(s) 409, 437, 958
Bse118I RCCGGY 1 cut(s) 1286
Bse1I ACTGG 3 cut(s) 129, 491, 1253
Bse21I CCTNAGG 2 cut(s) 462, 846
Bse3DI GCAATG 1 cut(s) 1272
BseDI CCNNGG 4 cut(s) 39, 325, 407, 1212
BseGI GGATG 4 cut(s) 73, 192, 1084, 1195
BseLI CCNNNNNNNGG 3 cut(s) 409, 437, 958
BseMI GCAATG 1 cut(s) 1272
BseMII CTCAG 4 cut(s) 453, 539, 837, 1263
BseNI ACTGG 3 cut(s) 129, 491, 1253
BseX3I CGGCCG 1 cut(s) 322
BseYI CCCAGC 1 cut(s) 519
BsgI GTGCAG 1 cut(s) 943
Bsh1236I CGCG 3 cut(s) 21, 327, 433
Bsh1285I CGRYCG 2 cut(s) 325, 1287
BshFI GGCC 3 cut(s) 45, 324, 614
BshTI ACCGGT 1 cut(s) 1286
BsiEI CGRYCG 2 cut(s) 325, 1287
BsiHKAI GWGCWC 1 cut(s) 14
BsiSI CCGG 4 cut(s) 113, 321, 800, 1287
BslFI GGGAC 1 cut(s) 568
BslI CCNNNNNNNGG 3 cut(s) 409, 437, 958
BsmAI GTCTC 3 cut(s) 29, 562, 1359
BsmFI GGGAC 1 cut(s) 568
BsnI GGCC 3 cut(s) 45, 324, 614
Bso31I GGTCTC 2 cut(s) 29, 562
Bsp1286I GDGCHC 1 cut(s) 14
Bsp143I GATC 3 cut(s) 544, 841, 1333
Bsp19I CCATGG 1 cut(s) 1212
BspANI GGCC 3 cut(s) 45, 324, 614
BspCNI CTCAG 4 cut(s) 454, 540, 838, 1264
BspFNI CGCG 3 cut(s) 21, 327, 433
BspHI TCATGA 1 cut(s) 532
BspLI GGNNCC 2 cut(s) 584, 798
BspMI ACCTGC 1 cut(s) 411
BspPI GGATC 1 cut(s) 836
BspQI GCTCTTC 1 cut(s) 2
BspTNI GGTCTC 2 cut(s) 29, 562
BsrDI GCAATG 1 cut(s) 1272
BsrFI RCCGGY 1 cut(s) 1286
BsrI ACTGG 3 cut(s) 129, 491, 1253
BssAI RCCGGY 1 cut(s) 1286
BssECI CCNNGG 4 cut(s) 39, 325, 407, 1212
BssMI GATC 3 cut(s) 544, 841, 1333
BssNI GRCGYC 1 cut(s) 183
BssSI CACGAG 1 cut(s) 932
BssT1I CCWWGG 2 cut(s) 39, 1212
Bst2BI CACGAG 1 cut(s) 932
Bst4CI ACNGT 1 cut(s) 96
Bst6I CTCTTC 7 cut(s) 2, 562, 692, 723, 1047, 1373, 1398
BstACI GRCGYC 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 216, 381
BstDEI CTNAG 4 cut(s) 462, 548, 846, 1272
BstDSI CCRYGG 3 cut(s) 325, 407, 1212
BstF5I GGATG 4 cut(s) 73, 192, 1084, 1195
BstFNI CGCG 3 cut(s) 21, 327, 433
BstHHI GCGC 1 cut(s) 435
BstKTI GATC 3 cut(s) 547, 844, 1336
BstMAI GTCTC 3 cut(s) 29, 562, 1359
BstMBI GATC 3 cut(s) 544, 841, 1333
BstMCI CGRYCG 2 cut(s) 325, 1287
BstMWI GCNNNNNNNGC 3 cut(s) 18, 432, 1217
BstNSI RCATGY 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 111
BstUI CGCG 3 cut(s) 21, 327, 433
BstV2I GAAGAC 1 cut(s) 1403
BstZI CGGCCG 1 cut(s) 322
Bsu36I CCTNAGG 2 cut(s) 462, 846
BsuI GTATCC 1 cut(s) 468
BsuRI GGCC 3 cut(s) 45, 324, 614
BtgI CCRYGG 3 cut(s) 325, 407, 1212
BtsCI GGATG 4 cut(s) 73, 192, 1084, 1195
BtsI GCAGTG 1 cut(s) 926
BtsIMutI CAGTG 2 cut(s) 926, 1126
BveI ACCTGC 1 cut(s) 411
Cac8I GCNNGC 2 cut(s) 216, 381
CciI TCATGA 1 cut(s) 532
CfoI GCGC 1 cut(s) 435
Cfr10I RCCGGY 1 cut(s) 1286
Cfr13I GGNCC 3 cut(s) 43, 582, 807
Cfr42I CCGCGG 1 cut(s) 328
CseI GACGC 1 cut(s) 191
Csp6I GTAC 4 cut(s) 541, 625, 790, 1408
CspAI ACCGGT 1 cut(s) 1286
CviAII CATG 9 cut(s) 80, 89, 209, 436, 533, 595, 1213, 1295, 1331
CviQI GTAC 4 cut(s) 541, 625, 790, 1408
DdeI CTNAG 4 cut(s) 462, 548, 846, 1272
DpnI GATC 3 cut(s) 546, 843, 1335
DpnII GATC 3 cut(s) 544, 841, 1333
DriI GACNNNNNGTC 1 cut(s) 1290
EaeI YGGCCR 1 cut(s) 322
EagI CGGCCG 1 cut(s) 322
Eam1104I CTCTTC 7 cut(s) 2, 562, 692, 723, 1047, 1373, 1398
Eam1105I GACNNNNNGTC 1 cut(s) 1290
EarI CTCTTC 7 cut(s) 2, 562, 692, 723, 1047, 1373, 1398
EciI GGCGGA 1 cut(s) 148
EclXI CGGCCG 1 cut(s) 322
Eco130I CCWWGG 2 cut(s) 39, 1212
Eco31I GGTCTC 2 cut(s) 29, 562
Eco32I GATATC 1 cut(s) 73
Eco47I GGWCC 2 cut(s) 582, 807
Eco52I CGGCCG 1 cut(s) 322
Eco81I CCTNAGG 2 cut(s) 462, 846
EcoRV GATATC 1 cut(s) 73
EcoT14I CCWWGG 2 cut(s) 39, 1212
ErhI CCWWGG 2 cut(s) 39, 1212
FaeI CATG 9 cut(s) 83, 92, 212, 439, 536, 598, 1216, 1298, 1334
FaqI GGGAC 1 cut(s) 568
FatI CATG 9 cut(s) 79, 88, 208, 435, 532, 594, 1212, 1294, 1330
FauI CCCGC 2 cut(s) 569, 593
FbaI TGATCA 1 cut(s) 1333
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 4 cut(s) 282, 325, 328, 780
FokI GGATG 4 cut(s) 80, 179, 1091, 1202
Fsp4HI GCNGC 4 cut(s) 282, 325, 328, 780
FspBI CTAG 4 cut(s) 170, 603, 783, 1208
GlaI GCGC 1 cut(s) 434
GluI GCNGC 4 cut(s) 282, 325, 328, 780
GsaI CCCAGC 1 cut(s) 523
GsuI CTGGAG 3 cut(s) 112, 221, 1110
HaeIII GGCC 3 cut(s) 45, 324, 614
HapII CCGG 4 cut(s) 113, 321, 800, 1287
HgaI GACGC 1 cut(s) 191
HhaI GCGC 1 cut(s) 435
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 9 cut(s) 83, 92, 212, 439, 536, 598, 1216, 1298, 1334
Hin6I GCGC 1 cut(s) 433
HinP1I GCGC 1 cut(s) 433
HinfI GANTC 5 cut(s) 227, 317, 550, 928, 1010
HpaII CCGG 4 cut(s) 113, 321, 800, 1287
HphI GGTGA 4 cut(s) 16, 851, 1075, 1405
Hpy166II GTNNAC 4 cut(s) 343, 421, 807, 1250
Hpy188I TCNGA 6 cut(s) 77, 316, 349, 549, 967, 1111
Hpy188III TCNNGA 7 cut(s) 238, 389, 446, 461, 533, 845, 932
Hpy8I GTNNAC 4 cut(s) 343, 421, 807, 1250
HpyAV CCTTC 7 cut(s) 229, 295, 604, 613, 892, 962, 1377
HpyCH4III ACNGT 1 cut(s) 96
HpyCH4V TGCA 3 cut(s) 685, 924, 982
HpyF10VI GCNNNNNNNGC 3 cut(s) 18, 432, 1217
HpyF3I CTNAG 4 cut(s) 462, 548, 846, 1272
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 9 cut(s) 83, 92, 212, 439, 536, 598, 1216, 1298, 1334
HspAI GCGC 1 cut(s) 433
Ksp22I TGATCA 1 cut(s) 1333
KspI CCGCGG 1 cut(s) 328
Kzo9I GATC 3 cut(s) 544, 841, 1333
LguI GCTCTTC 1 cut(s) 2
LmnI GCTCC 1 cut(s) 796
LweI GCATC 1 cut(s) 1297
MaeI CTAG 4 cut(s) 170, 603, 783, 1208
MaeIII GTNAC 1 cut(s) 1121
MalI GATC 3 cut(s) 546, 843, 1335
MboI GATC 3 cut(s) 544, 841, 1333
MhlI GDGCHC 1 cut(s) 14
MluCI AATT 3 cut(s) 164, 655, 999
MluI ACGCGT 1 cut(s) 19
MlyI GAGTC 4 cut(s) 221, 311, 559, 1019
MmeI TCCRAC 2 cut(s) 339, 750
MseI TTAA 3 cut(s) 755, 971, 1155
MspA1I CMGCKG 1 cut(s) 327
MspI CCGG 4 cut(s) 113, 321, 800, 1287
MspR9I CCNGG 1 cut(s) 113
MvnI CGCG 3 cut(s) 21, 327, 433
MwoI GCNNNNNNNGC 3 cut(s) 18, 432, 1217
NciI CCSGG 1 cut(s) 113
NcoI CCATGG 1 cut(s) 1212
NdeII GATC 3 cut(s) 544, 841, 1333
NlaIII CATG 9 cut(s) 83, 92, 212, 439, 536, 598, 1216, 1298, 1334
NlaIV GGNNCC 2 cut(s) 584, 798
NmuCI GTSAC 1 cut(s) 1121
NspI RCATGY 1 cut(s) 83
PagI TCATGA 1 cut(s) 532
PciSI GCTCTTC 1 cut(s) 2
PcsI WCGNNNNNNNCGW 2 cut(s) 294, 312
PfeI GAWTC 1 cut(s) 928
PinAI ACCGGT 1 cut(s) 1286
PkrI GCNGC 4 cut(s) 283, 326, 329, 781
PleI GAGTC 4 cut(s) 221, 311, 558, 1018
PpsI GAGTC 4 cut(s) 221, 311, 558, 1018
PsiI TTATAA 1 cut(s) 480
PspFI CCCAGC 1 cut(s) 519
PspN4I GGNNCC 2 cut(s) 584, 798
PspPI GGNCC 3 cut(s) 43, 582, 807
RsaI GTAC 4 cut(s) 542, 626, 791, 1409
RsaNI GTAC 4 cut(s) 541, 625, 790, 1408
SacII CCGCGG 1 cut(s) 328
SapI GCTCTTC 1 cut(s) 2
SaqAI TTAA 3 cut(s) 755, 971, 1155
SatI GCNGC 4 cut(s) 282, 325, 328, 780
Sau3AI GATC 3 cut(s) 544, 841, 1333
Sau96I GGNCC 3 cut(s) 43, 582, 807
ScaI AGTACT 1 cut(s) 1409
SchI GAGTC 4 cut(s) 221, 311, 559, 1019
ScrFI CCNGG 1 cut(s) 113
SduI GDGCHC 1 cut(s) 14
SfaNI GCATC 1 cut(s) 1297
Sfr303I CCGCGG 1 cut(s) 328
SgrBI CCGCGG 1 cut(s) 328
SinI GGWCC 2 cut(s) 582, 807
Sse9I AATT 3 cut(s) 164, 655, 999
SspMI CTAG 4 cut(s) 170, 603, 783, 1208
StyD4I CCNGG 1 cut(s) 111
StyI CCWWGG 2 cut(s) 39, 1212
TaaI ACNGT 1 cut(s) 96
TaqI TCGA 7 cut(s) 48, 62, 276, 288, 297, 941, 1013
TasI AATT 3 cut(s) 164, 655, 999
TatI WGTACW 2 cut(s) 624, 1407
TauI GCSGC 4 cut(s) 284, 327, 330, 782
TfiI GAWTC 1 cut(s) 928
Tru1I TTAA 3 cut(s) 755, 971, 1155
Tru9I TTAA 3 cut(s) 755, 971, 1155
TscAI CASTG 2 cut(s) 926, 1126
TseFI GTSAC 1 cut(s) 1121
Tsp45I GTSAC 1 cut(s) 1121
TspDTI ATGAA 5 cut(s) 105, 165, 611, 1095, 1364
TspRI CASTG 2 cut(s) 926, 1126
VpaK11BI GGWCC 2 cut(s) 582, 807
XapI RAATTY 2 cut(s) 164, 655
XceI RCATGY 1 cut(s) 83
XmiI GTMKAC 1 cut(s) 342
XspI CTAG 4 cut(s) 170, 603, 783, 1208
ZrmI AGTACT 1 cut(s) 1409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.