Rorug06G0166700

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0021460
Physical Location & Seq
Forward (+)
24096294 .. 24249478
153185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0166700.1

Sequence Viewer

Length: 1992 bp
ATGTCCCAAAAACAAAAGAACTTCATCAAGAGTTCATACCACGGTGGCATATATCTGCAGTTCCTAAAAGACACTCCAGCTAACAGTTTTTCTACTAACTTGGATGTTTTTGCACTATGGGCTTATGATGCTGCTCATGCACTGGCCATAGCAATTGAAGAAGTAACTTGGAGTACAAGTAACATTGATTTCCAAAAGTCACATACTGCTCCCAACAACTCGACAGATCTTGAAAATTTGGCGGTCTCTCAATATGGTCCAAAACTATGCAAACCACTCTCGACTACTAGATTTAATGGCATTGCTGGAGATTTTAGACTTGTTGATAGGCAACTTCAATCCTCAACTTTTCAGATTGTTAACATAAATGGTGGTGCAACAAGAACAATCGGATTTTGGACACCAGAAAATGGACTACGTAAAAAGTTGGGCTCCTCAGTAAACACAGGAAAATGTTCAATTTTCAATGGCAATCTGGGACCCATTATATGGCCAGGAGATTCTCTTTCTGTTCCCAAGGGATGGGAGATACCAACAAATGGCAAGAAGTTGAGAATTGCAGTTCCTCAGAAAGTTGGTTTTTCTGAGTTCGTTAAGATCACAAAAGATCCAAGCACAAACATAACAGATGTCTCTGGGTTCAGTATTGATGTCTTTGAGGCTGCGCTGGAGATATTGCCATATGCTCTTCCCTATGAATTTATTCCCTTTGCAAAGCCCGATGGCACCACTGCTGGCAATAACAATGATTTGTGCTATCAAGTTTATATTGGGAACTTTGATGCTATAGTGGGAGATACAACAATTACAGCAAATAGGTCCTTGTTTGTAGACTTTACCATGCCATATACAGAAGCTGGTATAGTCATGGTTGTGCCGGTCAGAGATAGCAATAGAAAGAGTGCATGGGCTTTCTTGAAGCCTTGGAGATGGGACCTGTGGTTTACAACTGCTTGTTTCTTCCTCTTCATTGGTTTCGTTGTTTGGGTTCTTGAACACCGAATTAATAAACACTTTCGAGGCCCTCCCTCACATCAAGTTGGTACCAGTATTTGGTTTTCTTTCTCCACCATGGTTTTTGCACAGACCGCAAGAGTTGTTAGCAACTGGGCTAGAGTTGTGATGATTATCTGGATATTTGTTCTACTAGTACTGACAATAAATTACACGGCCAGTCTAACTTCACTATACACCGTCCAAAAACTCCAGCCAACCGTTACTGATATAAATATTGTATTAAAGAAAGGGGATCAAGTTGGTCATGTAAGGAATTATGTTCGTGACATGTTGAAGCAAAAAGGTTTTAATGATTCAAAGCTTAAGCGTTTTACAACTGTGGAAGAAATTGATGACGCGCTTTCAAAAGGGAGTACAAATGGTGGAGTGGCTGGTGTTATTGATGAAACTCCCAACCTGAAGCTTTTTCTTGCAAAGTATTGCAACAAATATACCCTGGTTGGACCCTTCTTTAGAACTGATGGTTTTGCCTTTGCGTTTCCAAAAGGTTCCCCTTTTCTACAAGATGTTTCACAGGCAGTCCTAAACGTGACCGGGAGAGAGAAGATAATGAAAATTGAAAATAAATGGTTCAAGAAAGAAAACAGCTGTCAAGACTTGACTAACGCAAACATTAATAGCAATGGTCTTGGTGTTGATAGCTTTTGGGTCTTATTTCTCATTGTGGCGGGGGCAATACTAGCTCTGATAATATTTGTAGTTTCGTTTATTTACAGGCATAAGGTTATCTGGAGTTCAGAAGCCTCTATTTGGTGTAGGATTCAAAAAATGTTAACTATCTTCAATGAAAAAGACCTGAGCTATCATACTTTTAATATCAATGAAAAGGAAGATGAAGTGACTGCCTTACCTAACTCCTCCCCAAAAAGTTCTGTGCACCAGAATTTGAACCAGGGAAATTCAAATTCTGTATTTTCTGGTTCTGGAGAGCAACAAGCAGCAAGGGTCTCCATGAGTAGCTGCTGCAACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

663

Amino Acids

74.0

Weight (kDa)

8.95

Isoelectric Point (pI)

29.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 30 - 124 1.7e-09 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 202 - 293 9.9e-07 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 211 - 531 2.4e-09 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 311 - 564 1e-33 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021460)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0284771
rosa_rugosa Rorug06G0166700
rosa_samantha Rh6CG281600 Rh6CG281800 Rh6DG274600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1043
AccB1I GGYRCC 2 cut(s) 725, 1043
AccB7I CCANNNNNTGG 5 cut(s) 410, 489, 522, 539, 1053
AccI GTMKAC 1 cut(s) 831
AccII CGCG 1 cut(s) 1355
AciI CCGC 3 cut(s) 242, 1089, 1685
AclWI GGATC 2 cut(s) 602, 1257
AcoI YGGCCR 3 cut(s) 144, 491, 1170
AcsI RAATTY 5 cut(s) 235, 698, 1900, 1915, 1921
AcuI CTGAAG 1 cut(s) 1436
AfaI GTAC 4 cut(s) 175, 1045, 1152, 1372
AfiI CCNNNNNNNGG 6 cut(s) 410, 489, 522, 539, 1053, 1767
AflII CTTAAG 1 cut(s) 1319
AflIII ACRYGT 1 cut(s) 1284
AhlI ACTAGT 1 cut(s) 1147
AjnI CCWGG 3 cut(s) 493, 1452, 1908
AluBI AGCT 9 cut(s) 80, 857, 1318, 1420, 1605, 1659, 1700, 1818, 1977
AluI AGCT 9 cut(s) 80, 857, 1318, 1420, 1605, 1659, 1700, 1818, 1977
Alw21I GWGCWC 1 cut(s) 1896
Alw26I GTCTC 3 cut(s) 250, 637, 1969
Alw44I GTGCAC 1 cut(s) 1892
AlwI GGATC 2 cut(s) 602, 1257
AlwNI CAGNNNCTG 1 cut(s) 857
AoxI GGCC 4 cut(s) 144, 491, 1021, 1170
ApaLI GTGCAC 1 cut(s) 1892
ApeKI GCWGC 5 cut(s) 131, 662, 1955, 1977, 1980
ApoI RAATTY 5 cut(s) 235, 698, 1900, 1915, 1921
AseI ATTAAT 2 cut(s) 1005, 1632
Asp700I GAANNNNTTC 2 cut(s) 454, 702
Asp718I GGTACC 1 cut(s) 1043
AspLEI GCGC 2 cut(s) 667, 1357
AspS9I GGNCC 6 cut(s) 257, 479, 819, 934, 1022, 1460
AsuC2I CCSGG 1 cut(s) 1552
AvaII GGWCC 5 cut(s) 257, 479, 819, 934, 1460
BaeGI GKGCMC 1 cut(s) 1896
BalI TGGCCA 2 cut(s) 146, 493
BanI GGYRCC 2 cut(s) 725, 1043
BanII GRGCYC 1 cut(s) 434
Bbv12I GWGCWC 1 cut(s) 1896
BbvI GCAGC 5 cut(s) 118, 649, 1964, 1967, 1967
BccI CCATC 4 cut(s) 516, 716, 924, 1472
BceAI ACGGC 1 cut(s) 1185
BciT130I CCWGG 3 cut(s) 495, 1454, 1910
BcnI CCSGG 1 cut(s) 1552
BcoDI GTCTC 3 cut(s) 250, 637, 1969
BcuI ACTAGT 1 cut(s) 1147
BfaI CTAG 4 cut(s) 288, 1113, 1148, 1697
BfmI CTRYAG 2 cut(s) 56, 786
BfrI CTTAAG 1 cut(s) 1319
BglII AGATCT 1 cut(s) 226
BisI GCNGC 5 cut(s) 132, 663, 1956, 1978, 1981
BlsI GCNGC 5 cut(s) 133, 664, 1957, 1979, 1982
BmcAI AGTACT 1 cut(s) 1152
Bme1390I CCNGG 4 cut(s) 495, 1454, 1552, 1910
Bme18I GGWCC 5 cut(s) 257, 479, 819, 934, 1460
BmgT120I GGNCC 6 cut(s) 257, 479, 819, 934, 1022, 1460
BmiI GGNNCC 8 cut(s) 433, 480, 481, 727, 935, 1045, 1462, 1507
BmrFI CCNGG 4 cut(s) 495, 1454, 1552, 1910
BmrI ACTGGG 1 cut(s) 1117
BmsI GCATC 2 cut(s) 118, 772
BmuI ACTGGG 1 cut(s) 1117
BpmI CTGGAG 6 cut(s) 60, 327, 689, 1190, 1768, 1962
Bpu10I CCTNAGC 1 cut(s) 1814
BpuMI CCSGG 1 cut(s) 1552
BsaAI YACGTR 1 cut(s) 419
BsaBI GATNNNNATC 1 cut(s) 1127
BsaI GGTCTC 2 cut(s) 250, 1969
BsaJI CCNNGG 6 cut(s) 40, 516, 923, 1071, 1452, 1909
BsaXI ACNNNNNCTCC 2 cut(s) 163, 193
Bsc4I CCNNNNNNNGG 6 cut(s) 410, 489, 522, 539, 1053, 1767
Bse118I RCCGGY 1 cut(s) 877
Bse1I ACTGG 4 cut(s) 147, 1047, 1112, 1173
Bse3DI GCAATG 2 cut(s) 300, 1645
Bse8I GATNNNNATC 1 cut(s) 1127
BseBI CCWGG 3 cut(s) 495, 1454, 1910
BseDI CCNNGG 6 cut(s) 40, 516, 923, 1071, 1452, 1909
BseGI GGATG 2 cut(s) 109, 527
BseJI GATNNNNATC 1 cut(s) 1127
BseLI CCNNNNNNNGG 6 cut(s) 410, 489, 522, 539, 1053, 1767
BseMI GCAATG 2 cut(s) 300, 1645
BseMII CTCAG 4 cut(s) 450, 576, 581, 1805
BseNI ACTGG 4 cut(s) 147, 1047, 1112, 1173
BseRI GAGGAG 2 cut(s) 424, 1864
BseSI GKGCMC 1 cut(s) 1896
BseXI GCAGC 5 cut(s) 118, 649, 1964, 1967, 1967
Bsh1236I CGCG 1 cut(s) 1355
BshFI GGCC 4 cut(s) 146, 493, 1023, 1172
BshNI GGYRCC 2 cut(s) 725, 1043
BsiHKAI GWGCWC 1 cut(s) 1896
BsiSI CCGG 2 cut(s) 878, 1551
BslFI GGGAC 2 cut(s) 492, 947
BslI CCNNNNNNNGG 6 cut(s) 410, 489, 522, 539, 1053, 1767
BsmAI GTCTC 3 cut(s) 250, 637, 1969
BsmFI GGGAC 2 cut(s) 492, 947
BsnI GGCC 4 cut(s) 146, 493, 1023, 1172
Bso31I GGTCTC 2 cut(s) 250, 1969
Bsp1286I GDGCHC 2 cut(s) 434, 1896
Bsp143I GATC 4 cut(s) 226, 597, 607, 1249
Bsp19I CCATGG 1 cut(s) 1071
BspACI CCGC 3 cut(s) 242, 1089, 1685
BspANI GGCC 4 cut(s) 146, 493, 1023, 1172
BspCNI CTCAG 4 cut(s) 449, 577, 580, 1806
BspFNI CGCG 1 cut(s) 1355
BspLI GGNNCC 8 cut(s) 433, 480, 481, 727, 935, 1045, 1462, 1507
BspMAI CTGCAG 1 cut(s) 60
BspPI GGATC 2 cut(s) 602, 1257
BspQI GCTCTTC 1 cut(s) 693
BspT107I GGYRCC 2 cut(s) 725, 1043
BspTI CTTAAG 1 cut(s) 1319
BspTNI GGTCTC 2 cut(s) 250, 1969
BsrDI GCAATG 2 cut(s) 300, 1645
BsrFI RCCGGY 1 cut(s) 877
BsrI ACTGG 4 cut(s) 147, 1047, 1112, 1173
BssAI RCCGGY 1 cut(s) 877
BssECI CCNNGG 6 cut(s) 40, 516, 923, 1071, 1452, 1909
BssMI GATC 4 cut(s) 226, 597, 607, 1249
BssT1I CCWWGG 3 cut(s) 516, 923, 1071
Bst2UI CCWGG 3 cut(s) 495, 1454, 1910
Bst4CI ACNGT 5 cut(s) 44, 86, 1195, 1216, 1336
Bst6I CTCTTC 2 cut(s) 693, 971
BstAFI CTTAAG 1 cut(s) 1319
BstBAI YACGTR 1 cut(s) 419
BstC8I GCNNGC 1 cut(s) 736
BstDEI CTNAG 4 cut(s) 436, 567, 585, 1814
BstDSI CCRYGG 2 cut(s) 40, 1071
BstF5I GGATG 2 cut(s) 109, 527
BstFNI CGCG 1 cut(s) 1355
BstHHI GCGC 2 cut(s) 667, 1357
BstKTI GATC 4 cut(s) 229, 600, 610, 1252
BstMAI GTCTC 3 cut(s) 250, 637, 1969
BstMBI GATC 4 cut(s) 226, 597, 607, 1249
BstMWI GCNNNNNNNGC 5 cut(s) 119, 128, 137, 1088, 1697
BstNI CCWGG 3 cut(s) 495, 1454, 1910
BstNSI RCATGY 1 cut(s) 1288
BstSCI CCNGG 4 cut(s) 493, 1452, 1550, 1908
BstSFI CTRYAG 2 cut(s) 56, 786
BstSLI GKGCMC 1 cut(s) 1896
BstSNI TACGTA 1 cut(s) 419
BstUI CGCG 1 cut(s) 1355
BstV1I GCAGC 5 cut(s) 118, 649, 1964, 1967, 1967
BstX2I RGATCY 2 cut(s) 226, 607
BstYI RGATCY 2 cut(s) 226, 607
BsuRI GGCC 4 cut(s) 146, 493, 1023, 1172
BtgI CCRYGG 2 cut(s) 40, 1071
BtsCI GGATG 2 cut(s) 109, 527
BtsI GCAGTG 1 cut(s) 729
BtsIMutI CAGTG 2 cut(s) 140, 729
Cac8I GCNNGC 1 cut(s) 736
CaiI CAGNNNCTG 1 cut(s) 857
CfoI GCGC 2 cut(s) 667, 1357
Cfr10I RCCGGY 1 cut(s) 877
Cfr13I GGNCC 6 cut(s) 257, 479, 819, 934, 1022, 1460
CseI GACGC 1 cut(s) 1361
Csp6I GTAC 4 cut(s) 174, 1044, 1151, 1371
CviAII CATG 8 cut(s) 137, 841, 868, 906, 1072, 1262, 1285, 1969
CviQI GTAC 4 cut(s) 174, 1044, 1151, 1371
DdeI CTNAG 4 cut(s) 436, 567, 585, 1814
DpnI GATC 4 cut(s) 228, 599, 609, 1251
DpnII GATC 4 cut(s) 226, 597, 607, 1249
EaeI YGGCCR 3 cut(s) 144, 491, 1170
Eam1104I CTCTTC 2 cut(s) 693, 971
EarI CTCTTC 2 cut(s) 693, 971
Eco105I TACGTA 1 cut(s) 419
Eco130I CCWWGG 3 cut(s) 516, 923, 1071
Eco24I GRGCYC 1 cut(s) 434
Eco31I GGTCTC 2 cut(s) 250, 1969
Eco47I GGWCC 5 cut(s) 257, 479, 819, 934, 1460
Eco57I CTGAAG 1 cut(s) 1436
EcoO109I RGGNCCY 4 cut(s) 479, 819, 934, 1022
EcoRII CCWGG 3 cut(s) 493, 1452, 1908
EcoT14I CCWWGG 3 cut(s) 516, 923, 1071
EcoT38I GRGCYC 1 cut(s) 434
ErhI CCWWGG 3 cut(s) 516, 923, 1071
FaeI CATG 8 cut(s) 140, 844, 871, 909, 1075, 1265, 1288, 1972
FaqI GGGAC 2 cut(s) 492, 947
FatI CATG 8 cut(s) 136, 840, 867, 905, 1071, 1261, 1284, 1968
FauI CCCGC 1 cut(s) 1678
FauNDI CATATG 1 cut(s) 682
FblI GTMKAC 1 cut(s) 831
Fnu4HI GCNGC 5 cut(s) 132, 663, 1956, 1978, 1981
FokI GGATG 2 cut(s) 116, 534
FriOI GRGCYC 1 cut(s) 434
Fsp4HI GCNGC 5 cut(s) 132, 663, 1956, 1978, 1981
FspBI CTAG 4 cut(s) 288, 1113, 1148, 1697
GlaI GCGC 2 cut(s) 666, 1356
GluI GCNGC 5 cut(s) 132, 663, 1956, 1978, 1981
GsuI CTGGAG 6 cut(s) 60, 327, 689, 1190, 1768, 1962
HaeIII GGCC 4 cut(s) 146, 493, 1023, 1172
HapII CCGG 2 cut(s) 878, 1551
HgaI GACGC 1 cut(s) 1361
HhaI GCGC 2 cut(s) 667, 1357
Hin1II CATG 8 cut(s) 140, 844, 871, 909, 1075, 1265, 1288, 1972
Hin6I GCGC 2 cut(s) 665, 1355
HinP1I GCGC 2 cut(s) 665, 1355
HincII GTYRAC 2 cut(s) 361, 1791
HindII GTYRAC 2 cut(s) 361, 1791
HindIII AAGCTT 2 cut(s) 1316, 1418
HinfI GANTC 3 cut(s) 500, 1310, 1777
HpaI GTTAAC 2 cut(s) 361, 1791
HpaII CCGG 2 cut(s) 878, 1551
Hpy166II GTNNAC 6 cut(s) 361, 442, 832, 945, 1791, 1894
Hpy188I TCNGA 7 cut(s) 354, 392, 570, 586, 884, 1704, 1756
Hpy8I GTNNAC 6 cut(s) 361, 442, 832, 945, 1791, 1894
HpyAV CCTTC 1 cut(s) 1474
HpyCH4III ACNGT 5 cut(s) 44, 86, 1195, 1216, 1336
HpyCH4IV ACGT 2 cut(s) 418, 1545
HpyF10VI GCNNNNNNNGC 5 cut(s) 119, 128, 137, 1088, 1697
HpyF3I CTNAG 4 cut(s) 436, 567, 585, 1814
HpySE526I ACGT 2 cut(s) 418, 1545
Hsp92II CATG 8 cut(s) 140, 844, 871, 909, 1075, 1265, 1288, 1972
HspAI GCGC 2 cut(s) 665, 1355
KflI GGGWCCC 1 cut(s) 479
KpnI GGTACC 1 cut(s) 1047
KspAI GTTAAC 2 cut(s) 361, 1791
Kzo9I GATC 4 cut(s) 226, 597, 607, 1249
LguI GCTCTTC 1 cut(s) 693
LmnI GCTCC 2 cut(s) 214, 437
Lsp1109I GCAGC 5 cut(s) 118, 649, 1964, 1967, 1967
LweI GCATC 2 cut(s) 118, 772
MaeI CTAG 4 cut(s) 288, 1113, 1148, 1697
MaeII ACGT 2 cut(s) 418, 1545
MaeIII GTNAC 7 cut(s) 163, 179, 198, 1216, 1280, 1546, 1855
MalI GATC 4 cut(s) 228, 599, 609, 1251
MboI GATC 4 cut(s) 226, 597, 607, 1249
MboII GAAGA 8 cut(s) 170, 680, 952, 958, 1352, 1573, 1789, 1859
MfeI CAATTG 1 cut(s) 153
MflI RGATCY 2 cut(s) 226, 607
MhlI GDGCHC 2 cut(s) 434, 1896
MlsI TGGCCA 2 cut(s) 146, 493
MluNI TGGCCA 2 cut(s) 146, 493
MmeI TCCRAC 1 cut(s) 1438
Mox20I TGGCCA 2 cut(s) 146, 493
MroXI GAANNNNTTC 2 cut(s) 454, 702
MscI TGGCCA 2 cut(s) 146, 493
MslI CAYNNNNRTG 1 cut(s) 872
Msp20I TGGCCA 2 cut(s) 146, 493
MspA1I CMGCKG 1 cut(s) 1605
MspCI CTTAAG 1 cut(s) 1319
MspI CCGG 2 cut(s) 878, 1551
MspR9I CCNGG 4 cut(s) 495, 1454, 1552, 1910
MunI CAATTG 1 cut(s) 153
MvaI CCWGG 3 cut(s) 495, 1454, 1910
MvnI CGCG 1 cut(s) 1355
MwoI GCNNNNNNNGC 5 cut(s) 119, 128, 137, 1088, 1697
NciI CCSGG 1 cut(s) 1552
NcoI CCATGG 1 cut(s) 1071
NdeI CATATG 1 cut(s) 682
NdeII GATC 4 cut(s) 226, 597, 607, 1249
NlaIII CATG 8 cut(s) 140, 844, 871, 909, 1075, 1265, 1288, 1972
NlaIV GGNNCC 8 cut(s) 433, 480, 481, 727, 935, 1045, 1462, 1507
NmuCI GTSAC 4 cut(s) 198, 1280, 1546, 1855
NspI RCATGY 1 cut(s) 1288
PciI ACATGT 1 cut(s) 1284
PciSI GCTCTTC 1 cut(s) 693
PdmI GAANNNNTTC 2 cut(s) 454, 702
PfeI GAWTC 3 cut(s) 500, 1310, 1777
PflMI CCANNNNNTGG 5 cut(s) 410, 489, 522, 539, 1053
PkrI GCNGC 5 cut(s) 133, 664, 1957, 1979, 1982
Ppu21I YACGTR 1 cut(s) 419
PpuMI RGGWCCY 3 cut(s) 479, 819, 934
PscI ACATGT 1 cut(s) 1284
PshBI ATTAAT 2 cut(s) 1005, 1632
Psp5II RGGWCCY 3 cut(s) 479, 819, 934
Psp6I CCWGG 3 cut(s) 493, 1452, 1908
PspGI CCWGG 3 cut(s) 493, 1452, 1908
PspN4I GGNNCC 8 cut(s) 433, 480, 481, 727, 935, 1045, 1462, 1507
PspPI GGNCC 6 cut(s) 257, 479, 819, 934, 1022, 1460
PspPPI RGGWCCY 3 cut(s) 479, 819, 934
PstI CTGCAG 1 cut(s) 60
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 2 cut(s) 226, 607
PvuII CAGCTG 1 cut(s) 1605
RsaI GTAC 4 cut(s) 175, 1045, 1152, 1372
RsaNI GTAC 4 cut(s) 174, 1044, 1151, 1371
RseI CAYNNNNRTG 1 cut(s) 872
SapI GCTCTTC 1 cut(s) 693
SatI GCNGC 5 cut(s) 132, 663, 1956, 1978, 1981
Sau3AI GATC 4 cut(s) 226, 597, 607, 1249
Sau96I GGNCC 6 cut(s) 257, 479, 819, 934, 1022, 1460
ScaI AGTACT 1 cut(s) 1152
ScrFI CCNGG 4 cut(s) 495, 1454, 1552, 1910
SduI GDGCHC 2 cut(s) 434, 1896
SfaNI GCATC 2 cut(s) 118, 772
SfcI CTRYAG 2 cut(s) 56, 786
SinI GGWCC 5 cut(s) 257, 479, 819, 934, 1460
SmiMI CAYNNNNRTG 1 cut(s) 872
SmlI CTYRAG 1 cut(s) 1319
SmoI CTYRAG 1 cut(s) 1319
SnaBI TACGTA 1 cut(s) 419
SpeI ACTAGT 1 cut(s) 1147
SsiI CCGC 3 cut(s) 242, 1089, 1685
SspI AATATT 2 cut(s) 1231, 1710
SspMI CTAG 4 cut(s) 288, 1113, 1148, 1697
StyD4I CCNGG 4 cut(s) 493, 1452, 1550, 1908
StyI CCWWGG 3 cut(s) 516, 923, 1071
TaaI ACNGT 5 cut(s) 44, 86, 1195, 1216, 1336
TaiI ACGT 2 cut(s) 421, 1548
TaqI TCGA 3 cut(s) 221, 281, 1018
TatI WGTACW 3 cut(s) 173, 1150, 1370
TfiI GAWTC 3 cut(s) 500, 1310, 1777
TscAI CASTG 2 cut(s) 147, 736
TseFI GTSAC 4 cut(s) 198, 1280, 1546, 1855
TseI GCWGC 5 cut(s) 131, 662, 1955, 1977, 1980
Tsp45I GTSAC 4 cut(s) 198, 1280, 1546, 1855
TspDTI ATGAA 9 cut(s) 13, 24, 711, 958, 1416, 1583, 1818, 1854, 1866
TspRI CASTG 2 cut(s) 147, 736
Van91I CCANNNNNTGG 5 cut(s) 410, 489, 522, 539, 1053
Vha464I CTTAAG 1 cut(s) 1319
VneI GTGCAC 1 cut(s) 1892
VpaK11BI GGWCC 5 cut(s) 257, 479, 819, 934, 1460
VspI ATTAAT 2 cut(s) 1005, 1632
XapI RAATTY 5 cut(s) 235, 698, 1900, 1915, 1921
XceI RCATGY 1 cut(s) 1288
XmiI GTMKAC 1 cut(s) 831
XmnI GAANNNNTTC 2 cut(s) 454, 702
XspI CTAG 4 cut(s) 288, 1113, 1148, 1697
ZrmI AGTACT 1 cut(s) 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.