Rorug06G0465500

AtCASP,CASP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0014481
Physical Location & Seq
Reverse (-)
59024308 .. 59025793
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0465500.1

Sequence Viewer

Length: 1071 bp
ATGGCGAAAAAATTAGGGAAAGTTTCTTTGCTTTGCGATGAAGAACTTGCCCTAATCCTCACCAAGCTTCTCGACCCAGCTGATAGAAAATCCTTCTCCCAGGTCTGCAAACAATGGCTTAGAGTCGAGGGTCTAAACCGATCATCAATTCGTCTTCTCCGACCCGATTTTCTCCGTCAGGCACTGCCTAGGTTCCCAAACATAGTCACCTTCGAAACACCCAACTCTATCTCCGACACCGATCTCGAGTTCATATCCCAGACATGTCCCAAAATCGAGGTCCTCAACCTTAATTTGGTGACCACGTCGTGGGATCTTGGTCTCAAATGTTTAGGTCTACCGGCTCTGGCAAATGGGTGTCCGAAATTGTCCAAGGTTATACTTACAGGCAGAAGCTCTATTACGGATCAGATATCTGTTTCGCTAATAAACTTGGCGAAAAATTTGACGTATTTGGACTTGGGGTGTTGTTTGGAGGTCTCGGACAAATCACTTGAAGCTATTGGGTTTTGTAGTTGGATTCGTTATTTGAGTTTGGAATGGTGTTGTCAGATCACGGATAAAGGATTGGGTTTTCTTGCAAATGGGTCTTGCTCGAAAACCCTCAAGACATTGATCCTTGCTTGGTGTGAAGGGATTACAGATGTTGGGGTATCGCATTTGCAGAGAATGCATTGCTTGGAACACCTTAATTTGGCCAATTGCGGCCGAAGGATCACGGACACTGGAGGTGTGGCAATCTCGGCCATTTCAACCCTCAAGAAATTAAGCTTGATTGCGCTGGTAAAAGTGTCGGACCGCACCATTGTTGCTCTCGCTGAGAACTGCCTCAACTTGGAAGTTATTGATTTAAGTGATTGTAGACTGACTGGAGTTAGTATACGTGCATTTTCGAGTCACAAGTTGTTGCAATCTATTGTTCTGCGGTTCTGTAATGTTAGTGGGCATAATTTGGAACACTTGGTGCGTGGATGCCCGTCACTGAAATCTGTTGTGCTGGATTCTGAGACTTTAATTCAGATGAAGCCTCAGCTGGGAAAAAGAACTAGAAAAGTTGTGACAATGTGTTAG

Protein Analysis

356

Amino Acids

39.21

Weight (kDa)

8.81

Isoelectric Point (pI)

31.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box_5 PF18511 13 - 48 1.9e-06 F-box
DUF7885 PF25372 98 - 221 2.8e-09 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18480
fragaria_vesca FvH4_5g12492 FvH4_5g12492 FvH4_5g12492
malus_domestica MD10G1081900.v1.1
prunus_persica Prupe.8G104400_v2.0.a1
pyrus_communis pycom05g06100
rosa_chinensis RchiOBHm_Chr7g0184861
rosa_laevigata RLG00000004948
rosa_multiflora Rmu_ssc0000289.1_g000012
rosa_roxburghii Rroxscaffold_3G00269320
rosa_rugosa Rorug06G0465500
rosa_samantha Rh7AG077400 Rh7BG069900 Rh7CG070700 Rh7DG070100
rosa_wichuraiana Rw7G005800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 309
AccI GTMKAC 3 cut(s) 337, 862, 880
AciI CCGC 3 cut(s) 705, 799, 925
AclWI GGATC 4 cut(s) 321, 414, 610, 722
AcoI YGGCCR 3 cut(s) 696, 706, 744
AcsI RAATTY 1 cut(s) 442
AdeI CACNNNGTG 2 cut(s) 309, 964
AfiI CCNNNNNNNGG 5 cut(s) 295, 309, 694, 835, 1034
AflIII ACRYGT 1 cut(s) 263
AgsI TTSAA 2 cut(s) 497, 753
AjiI CACGTC 1 cut(s) 306
AjnI CCWGG 1 cut(s) 99
AluBI AGCT 6 cut(s) 67, 80, 396, 500, 771, 1033
AluI AGCT 6 cut(s) 67, 80, 396, 500, 771, 1033
Alw26I GTCTC 3 cut(s) 326, 484, 1001
AlwI GGATC 4 cut(s) 321, 414, 610, 722
AlwNI CAGNNNCTG 1 cut(s) 184
Ama87I CYCGRG 1 cut(s) 245
AoxI GGCC 3 cut(s) 696, 706, 744
ApoI RAATTY 1 cut(s) 442
ArsI GACNNNNNNTTYG 2 cut(s) 264, 296
AspA2I CCTAGG 1 cut(s) 188
AspLEI GCGC 1 cut(s) 781
AspS9I GGNCC 2 cut(s) 280, 796
AsuHPI GGTGA 3 cut(s) 52, 199, 310
AsuII TTCGAA 1 cut(s) 213
AvaI CYCGRG 1 cut(s) 245
AvaII GGWCC 2 cut(s) 280, 796
AvrII CCTAGG 1 cut(s) 188
BalI TGGCCA 1 cut(s) 698
BarI GAAGNNNNNNTAC 2 cut(s) 385, 417
BbsI GAAGAC 1 cut(s) 146
BbvCI CCTCAGC 1 cut(s) 1029
BciT130I CCWGG 1 cut(s) 101
BcoDI GTCTC 3 cut(s) 326, 484, 1001
BfaI CTAG 2 cut(s) 189, 1047
BisI GCNGC 1 cut(s) 706
BlnI CCTAGG 1 cut(s) 188
BlsI GCNGC 1 cut(s) 707
Bme1390I CCNGG 1 cut(s) 101
Bme18I GGWCC 2 cut(s) 280, 796
BmeT110I CYCGRG 1 cut(s) 245
BmgBI CACGTC 1 cut(s) 306
BmgT120I GGNCC 2 cut(s) 280, 796
BmiI GGNNCC 1 cut(s) 194
BmrFI CCNGG 1 cut(s) 101
BmsI GCATC 1 cut(s) 962
BpiI GAAGAC 1 cut(s) 146
BpmI CTGGAG 2 cut(s) 747, 891
Bpu10I CCTNAGC 1 cut(s) 1029
Bpu14I TTCGAA 1 cut(s) 213
BpuEI CTTGAG 2 cut(s) 590, 743
BsaAI YACGTR 1 cut(s) 884
BsaI GGTCTC 2 cut(s) 326, 484
BsaJI CCNNGG 3 cut(s) 99, 188, 372
BsaXI ACNNNNNCTCC 2 cut(s) 215, 245
Bsc4I CCNNNNNNNGG 5 cut(s) 295, 309, 694, 835, 1034
Bse118I RCCGGY 1 cut(s) 340
Bse1I ACTGG 2 cut(s) 730, 874
Bse3DI GCAATG 1 cut(s) 673
BseBI CCWGG 1 cut(s) 101
BseDI CCNNGG 3 cut(s) 99, 188, 372
BseGI GGATG 1 cut(s) 977
BseLI CCNNNNNNNGG 5 cut(s) 295, 309, 694, 835, 1034
BseMI GCAATG 1 cut(s) 673
BseMII CTCAG 3 cut(s) 810, 996, 1043
BseNI ACTGG 2 cut(s) 730, 874
BseX3I CGGCCG 1 cut(s) 706
BseYI CCCAGC 2 cut(s) 76, 1033
Bsh1285I CGRYCG 1 cut(s) 709
BshFI GGCC 3 cut(s) 698, 708, 746
BsiEI CGRYCG 1 cut(s) 709
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 1 cut(s) 341
BslFI GGGAC 1 cut(s) 252
BslI CCNNNNNNNGG 5 cut(s) 295, 309, 694, 835, 1034
BsmAI GTCTC 3 cut(s) 326, 484, 1001
BsmFI GGGAC 1 cut(s) 252
BsmI GAATGC 1 cut(s) 675
BsnI GGCC 3 cut(s) 698, 708, 746
Bso31I GGTCTC 2 cut(s) 326, 484
BsoBI CYCGRG 1 cut(s) 245
Bsp119I TTCGAA 1 cut(s) 213
Bsp143I GATC 7 cut(s) 140, 241, 313, 406, 552, 615, 714
BspACI CCGC 3 cut(s) 705, 799, 925
BspANI GGCC 3 cut(s) 698, 708, 746
BspCNI CTCAG 3 cut(s) 811, 997, 1042
BspLI GGNNCC 1 cut(s) 194
BspPI GGATC 4 cut(s) 321, 414, 610, 722
BspT104I TTCGAA 1 cut(s) 213
BspTNI GGTCTC 2 cut(s) 326, 484
BsrDI GCAATG 1 cut(s) 673
BsrFI RCCGGY 1 cut(s) 340
BsrI ACTGG 2 cut(s) 730, 874
BssAI RCCGGY 1 cut(s) 340
BssECI CCNNGG 3 cut(s) 99, 188, 372
BssMI GATC 7 cut(s) 140, 241, 313, 406, 552, 615, 714
BssNAI GTATAC 1 cut(s) 881
BssT1I CCWWGG 2 cut(s) 188, 372
Bst1107I GTATAC 1 cut(s) 881
Bst2UI CCWGG 1 cut(s) 101
BstAPI GCANNNNNTGC 1 cut(s) 670
BstBAI YACGTR 1 cut(s) 884
BstBI TTCGAA 1 cut(s) 213
BstDEI CTNAG 4 cut(s) 119, 819, 1005, 1029
BstEII GGTNACC 1 cut(s) 298
BstF5I GGATG 1 cut(s) 977
BstHHI GCGC 1 cut(s) 781
BstKTI GATC 7 cut(s) 143, 244, 316, 409, 555, 618, 717
BstMAI GTCTC 3 cut(s) 326, 484, 1001
BstMBI GATC 7 cut(s) 140, 241, 313, 406, 552, 615, 714
BstMCI CGRYCG 1 cut(s) 709
BstMWI GCNNNNNNNGC 2 cut(s) 670, 743
BstNI CCWGG 1 cut(s) 101
BstNSI RCATGY 1 cut(s) 267
BstPI GGTNACC 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 99
BstV2I GAAGAC 1 cut(s) 146
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BstZ17I GTATAC 1 cut(s) 881
BstZI CGGCCG 1 cut(s) 706
BsuRI GGCC 3 cut(s) 698, 708, 746
BtgZI GCGATG 1 cut(s) 51
BtrI CACGTC 1 cut(s) 306
BtsCI GGATG 1 cut(s) 977
BtsI GCAGTG 1 cut(s) 182
BtsIMutI CAGTG 3 cut(s) 182, 723, 980
CaiI CAGNNNCTG 1 cut(s) 184
CfoI GCGC 1 cut(s) 781
Cfr10I RCCGGY 1 cut(s) 340
Cfr13I GGNCC 2 cut(s) 280, 796
CpoI CGGWCCG 1 cut(s) 796
CspI CGGWCCG 1 cut(s) 796
CviAII CATG 1 cut(s) 264
DdeI CTNAG 4 cut(s) 119, 819, 1005, 1029
DpnI GATC 7 cut(s) 142, 243, 315, 408, 554, 617, 716
DpnII GATC 7 cut(s) 140, 241, 313, 406, 552, 615, 714
DraIII CACNNNGTG 2 cut(s) 309, 964
EaeI YGGCCR 3 cut(s) 696, 706, 744
EagI CGGCCG 1 cut(s) 706
EclXI CGGCCG 1 cut(s) 706
Eco130I CCWWGG 2 cut(s) 188, 372
Eco31I GGTCTC 2 cut(s) 326, 484
Eco32I GATATC 1 cut(s) 414
Eco47I GGWCC 2 cut(s) 280, 796
Eco52I CGGCCG 1 cut(s) 706
Eco88I CYCGRG 1 cut(s) 245
Eco91I GGTNACC 1 cut(s) 298
EcoO109I RGGNCCY 1 cut(s) 280
EcoO65I GGTNACC 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 99
EcoRV GATATC 1 cut(s) 414
EcoT14I CCWWGG 2 cut(s) 188, 372
EcoT22I ATGCAT 1 cut(s) 675
ErhI CCWWGG 2 cut(s) 188, 372
FaeI CATG 1 cut(s) 267
FaiI YATR 6 cut(s) 203, 254, 265, 380, 881, 948
FaqI GGGAC 1 cut(s) 252
FatI CATG 1 cut(s) 263
FblI GTMKAC 3 cut(s) 337, 862, 880
Fnu4HI GCNGC 1 cut(s) 706
FokI GGATG 1 cut(s) 984
Fsp4HI GCNGC 1 cut(s) 706
FspBI CTAG 2 cut(s) 189, 1047
GlaI GCGC 1 cut(s) 780
GluI GCNGC 1 cut(s) 706
GsaI CCCAGC 2 cut(s) 80, 1037
GsuI CTGGAG 2 cut(s) 747, 891
HaeIII GGCC 3 cut(s) 698, 708, 746
HapII CCGG 1 cut(s) 341
HhaI GCGC 1 cut(s) 781
Hin1II CATG 1 cut(s) 267
Hin6I GCGC 1 cut(s) 779
HinP1I GCGC 1 cut(s) 779
HindIII AAGCTT 2 cut(s) 65, 769
HinfI GANTC 4 cut(s) 123, 520, 895, 1001
HpaII CCGG 1 cut(s) 341
HphI GGTGA 3 cut(s) 52, 199, 310
Hpy166II GTNNAC 3 cut(s) 338, 863, 881
Hpy188I TCNGA 9 cut(s) 161, 235, 363, 411, 484, 552, 796, 1006, 1020
Hpy188III TCNNGA 4 cut(s) 71, 245, 607, 760
Hpy8I GTNNAC 3 cut(s) 338, 863, 881
Hpy99I CGWCG 1 cut(s) 310
HpyAV CCTTC 4 cut(s) 103, 220, 626, 705
HpyCH4IV ACGT 3 cut(s) 305, 449, 883
HpyCH4V TGCA 6 cut(s) 108, 581, 664, 673, 887, 910
HpyF10VI GCNNNNNNNGC 2 cut(s) 670, 743
HpyF3I CTNAG 4 cut(s) 119, 819, 1005, 1029
HpySE526I ACGT 3 cut(s) 305, 449, 883
Hsp92II CATG 1 cut(s) 267
HspAI GCGC 1 cut(s) 779
Kzo9I GATC 7 cut(s) 140, 241, 313, 406, 552, 615, 714
LweI GCATC 1 cut(s) 962
MaeI CTAG 2 cut(s) 189, 1047
MaeII ACGT 3 cut(s) 305, 449, 883
MaeIII GTNAC 5 cut(s) 205, 298, 896, 978, 1057
MalI GATC 7 cut(s) 142, 243, 315, 408, 554, 617, 716
MboI GATC 7 cut(s) 140, 241, 313, 406, 552, 615, 714
MboII GAAGA 2 cut(s) 53, 146
MfeI CAATTG 1 cut(s) 700
MflI RGATCY 1 cut(s) 313
MlsI TGGCCA 1 cut(s) 698
MluNI TGGCCA 1 cut(s) 698
MlyI GAGTC 2 cut(s) 132, 904
MmeI TCCRAC 4 cut(s) 184, 258, 497, 774
Mox20I TGGCCA 1 cut(s) 698
Mph1103I ATGCAT 1 cut(s) 675
MscI TGGCCA 1 cut(s) 698
MseI TTAA 5 cut(s) 291, 690, 767, 851, 1013
Msp20I TGGCCA 1 cut(s) 698
MspA1I CMGCKG 2 cut(s) 80, 1033
MspI CCGG 1 cut(s) 341
MspR9I CCNGG 1 cut(s) 101
MunI CAATTG 1 cut(s) 700
Mva1269I GAATGC 1 cut(s) 675
MvaI CCWGG 1 cut(s) 101
MwoI GCNNNNNNNGC 2 cut(s) 670, 743
NdeII GATC 7 cut(s) 140, 241, 313, 406, 552, 615, 714
NlaIII CATG 1 cut(s) 267
NlaIV GGNNCC 1 cut(s) 194
NmeAIII GCCGAG 1 cut(s) 722
NmuCI GTSAC 5 cut(s) 205, 298, 896, 978, 1057
NsiI ATGCAT 1 cut(s) 675
NspI RCATGY 1 cut(s) 267
NspV TTCGAA 1 cut(s) 213
PaeR7I CTCGAG 1 cut(s) 245
PciI ACATGT 1 cut(s) 263
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PctI GAATGC 1 cut(s) 675
PfeI GAWTC 2 cut(s) 520, 1001
PflFI GACNNNGTC 1 cut(s) 304
PflMI CCANNNNNTGG 1 cut(s) 309
PkrI GCNGC 1 cut(s) 707
PleI GAGTC 2 cut(s) 131, 903
PpsI GAGTC 2 cut(s) 131, 903
Ppu21I YACGTR 1 cut(s) 884
PpuMI RGGWCCY 1 cut(s) 280
PscI ACATGT 1 cut(s) 263
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 99
PspEI GGTNACC 1 cut(s) 298
PspFI CCCAGC 2 cut(s) 76, 1033
PspGI CCWGG 1 cut(s) 99
PspN4I GGNNCC 1 cut(s) 194
PspPI GGNCC 2 cut(s) 280, 796
PspPPI RGGWCCY 1 cut(s) 280
PstNI CAGNNNCTG 1 cut(s) 184
PsuI RGATCY 1 cut(s) 313
PsyI GACNNNGTC 1 cut(s) 304
PvuII CAGCTG 2 cut(s) 80, 1033
Rsr2I CGGWCCG 1 cut(s) 796
RsrII CGGWCCG 1 cut(s) 796
SaqAI TTAA 5 cut(s) 291, 690, 767, 851, 1013
SatI GCNGC 1 cut(s) 706
Sau3AI GATC 7 cut(s) 140, 241, 313, 406, 552, 615, 714
Sau96I GGNCC 2 cut(s) 280, 796
SchI GAGTC 2 cut(s) 132, 904
ScrFI CCNGG 1 cut(s) 101
SfaNI GCATC 1 cut(s) 962
Sfr274I CTCGAG 1 cut(s) 245
SfuI TTCGAA 1 cut(s) 213
SinI GGWCC 2 cut(s) 280, 796
SlaI CTCGAG 1 cut(s) 245
SmlI CTYRAG 3 cut(s) 245, 605, 758
SmoI CTYRAG 3 cut(s) 245, 605, 758
SsiI CCGC 3 cut(s) 705, 799, 925
SspMI CTAG 2 cut(s) 189, 1047
StyD4I CCNGG 1 cut(s) 99
StyI CCWWGG 2 cut(s) 188, 372
TaiI ACGT 3 cut(s) 308, 452, 886
TaqI TCGA 7 cut(s) 72, 126, 213, 246, 276, 596, 893
TauI GCSGC 1 cut(s) 708
TfiI GAWTC 2 cut(s) 520, 1001
Tru1I TTAA 5 cut(s) 291, 690, 767, 851, 1013
Tru9I TTAA 5 cut(s) 291, 690, 767, 851, 1013
TscAI CASTG 3 cut(s) 189, 730, 987
TseFI GTSAC 5 cut(s) 205, 298, 896, 978, 1057
Tsp45I GTSAC 5 cut(s) 205, 298, 896, 978, 1057
TspDTI ATGAA 3 cut(s) 54, 241, 1037
TspGWI ACGGA 4 cut(s) 164, 419, 572, 734
TspRI CASTG 3 cut(s) 189, 730, 987
Tth111I GACNNNGTC 1 cut(s) 304
Van91I CCANNNNNTGG 1 cut(s) 309
VpaK11BI GGWCC 2 cut(s) 280, 796
XapI RAATTY 1 cut(s) 442
XceI RCATGY 1 cut(s) 267
XhoI CTCGAG 1 cut(s) 245
XmaJI CCTAGG 1 cut(s) 188
XmiI GTMKAC 3 cut(s) 337, 862, 880
XspI CTAG 2 cut(s) 189, 1047
Zsp2I ATGCAT 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.