Rh1AG121900

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0019726
Physical Location & Seq
Forward (+)
22797804 .. 22798274
471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG121900.1

Sequence Viewer

Length: 471 bp
ATGAGCATTATAAGTGGATTAGCTTATGTTCTTGTGATACTAATTATAGTTCTCTCCCTTGCTTCCTACTTCTGCTCTAAACGCGCACACTTATCCGCAGACACATTTGATCATTCCTCCTCATCTATAGCCGGGGACCAAACCAGCAGTAGTGTACAAGCAGGAGGAGCTGGACTTGATGAAGCCACTATAACTAGTTACCCAAAGCTTGCTTATTCCAAAGTAAAGCTCCAATATGGAAACTCAACAGCTTGTTGCTGCTCTATTTGTTTGGCTGATTACAAGGAAACCGATATGCTCTTGGTGTTGCCTCATTGCGGTCATCTCTTCCATGTCAAGTGTGTGTATCCATGGCTTCGGCTTCACGCTACGTGCCCAATATGCAGGAACTCGCCTTCTCCACCTTCTCTTTGTATTTCTTGTAGTCAAATAACCCCCTTGCCTAACACTCCAGGAGTAATTAGCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

156

Amino Acids

16.63

Weight (kDa)

7.52

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 84 - 129 1.6e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 86 - 129 9.6e-12 Ring finger domain
zf-RING_11 PF17123 87 - 115 1.1e-09 RING-like zinc finger
zf-C3HC4 PF00097 87 - 128 3.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 87 - 128 1.7e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019726)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g05260
rosa_chinensis RchiOBHm_Chr1g0332051
rosa_laevigata RLG00000029696
rosa_roxburghii Rroxscaffold_4G00318420
rosa_samantha Rh1AG121900 Rh1BG093300 Rh1DG127600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 11
AccII CGCG 1 cut(s) 84
AciI CCGC 2 cut(s) 96, 318
AfaI GTAC 1 cut(s) 156
AfiI CCNNNNNNNGG 1 cut(s) 317
AhlI ACTAGT 1 cut(s) 194
AjnI CCWGG 1 cut(s) 451
AluBI AGCT 5 cut(s) 23, 170, 208, 229, 251
AluI AGCT 5 cut(s) 23, 170, 208, 229, 251
ApeKI GCWGC 1 cut(s) 258
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 136
AsuC2I CCSGG 1 cut(s) 133
AvaII GGWCC 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 377
BbvI GCAGC 1 cut(s) 245
BciT130I CCWGG 1 cut(s) 453
BciVI GTATCC 1 cut(s) 357
BclI TGATCA 1 cut(s) 109
BcnI CCSGG 1 cut(s) 133
BcuI ACTAGT 1 cut(s) 194
BfaI CTAG 1 cut(s) 195
BfmI CTRYAG 1 cut(s) 126
BfuI GTATCC 1 cut(s) 357
BisI GCNGC 1 cut(s) 259
BlsI GCNGC 1 cut(s) 260
Bme1390I CCNGG 2 cut(s) 133, 453
Bme18I GGWCC 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 1 cut(s) 137
BmrFI CCNGG 2 cut(s) 133, 453
BpmI CTGGAG 1 cut(s) 435
BpuMI CCSGG 1 cut(s) 133
BsaAI YACGTR 1 cut(s) 372
BsaJI CCNNGG 2 cut(s) 132, 350
Bsc4I CCNNNNNNNGG 1 cut(s) 317
Bse3DI GCAATG 1 cut(s) 313
BseBI CCWGG 1 cut(s) 453
BseDI CCNNGG 2 cut(s) 132, 350
BseLI CCNNNNNNNGG 1 cut(s) 317
BseMI GCAATG 1 cut(s) 313
BseRI GAGGAG 2 cut(s) 109, 180
BseSI GKGCMC 1 cut(s) 377
BseXI GCAGC 1 cut(s) 245
Bsh1236I CGCG 1 cut(s) 84
BsiSI CCGG 1 cut(s) 132
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 317
BsmFI GGGAC 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 377
Bsp1407I TGTACA 1 cut(s) 154
Bsp143I GATC 1 cut(s) 109
Bsp19I CCATGG 1 cut(s) 350
BspACI CCGC 2 cut(s) 96, 318
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 313
BsrGI TGTACA 1 cut(s) 154
BssECI CCNNGG 2 cut(s) 132, 350
BssMI GATC 1 cut(s) 109
BssT1I CCWWGG 1 cut(s) 350
Bst2UI CCWGG 1 cut(s) 453
Bst6I CTCTTC 1 cut(s) 332
BstAUI TGTACA 1 cut(s) 154
BstBAI YACGTR 1 cut(s) 372
BstC8I GCNNGC 1 cut(s) 210
BstDSI CCRYGG 1 cut(s) 350
BstFNI CGCG 1 cut(s) 84
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 1 cut(s) 112
BstMBI GATC 1 cut(s) 109
BstMWI GCNNNNNNNGC 3 cut(s) 81, 167, 381
BstNI CCWGG 1 cut(s) 453
BstSCI CCNGG 2 cut(s) 131, 451
BstSFI CTRYAG 1 cut(s) 126
BstSLI GKGCMC 1 cut(s) 377
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 245
BsuI GTATCC 1 cut(s) 357
BtgI CCRYGG 1 cut(s) 350
Cac8I GCNNGC 1 cut(s) 210
CfoI GCGC 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 136
Csp6I GTAC 1 cut(s) 155
CviAII CATG 2 cut(s) 332, 351
CviQI GTAC 1 cut(s) 155
DpnI GATC 1 cut(s) 111
DpnII GATC 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 332
EarI CTCTTC 1 cut(s) 332
Eco130I CCWWGG 1 cut(s) 350
Eco47I GGWCC 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 350
ErhI CCWWGG 1 cut(s) 350
FaeI CATG 2 cut(s) 335, 354
FaqI GGGAC 1 cut(s) 149
FatI CATG 2 cut(s) 331, 350
FbaI TGATCA 1 cut(s) 109
Fnu4HI GCNGC 1 cut(s) 259
Fsp4HI GCNGC 1 cut(s) 259
FspBI CTAG 1 cut(s) 195
GlaI GCGC 1 cut(s) 85
GluI GCNGC 1 cut(s) 259
GsuI CTGGAG 1 cut(s) 435
HapII CCGG 1 cut(s) 132
HhaI GCGC 1 cut(s) 86
Hin1II CATG 2 cut(s) 335, 354
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HindIII AAGCTT 1 cut(s) 206
HpaII CCGG 1 cut(s) 132
Hpy166II GTNNAC 1 cut(s) 155
Hpy8I GTNNAC 1 cut(s) 155
HpyAV CCTTC 2 cut(s) 405, 414
HpyCH4IV ACGT 1 cut(s) 371
HpyCH4V TGCA 1 cut(s) 384
HpyF10VI GCNNNNNNNGC 3 cut(s) 81, 167, 381
HpySE526I ACGT 1 cut(s) 371
Hsp92II CATG 2 cut(s) 335, 354
HspAI GCGC 1 cut(s) 84
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 1 cut(s) 109
LmnI GCTCC 2 cut(s) 167, 234
LpnPI CCDG 7 cut(s) 145, 147, 156, 157, 370, 438, 465
Lsp1109I GCAGC 1 cut(s) 245
MaeI CTAG 1 cut(s) 195
MaeII ACGT 1 cut(s) 371
MaeIII GTNAC 1 cut(s) 197
MalI GATC 1 cut(s) 111
MboI GATC 1 cut(s) 109
MboII GAAGA 1 cut(s) 319
MhlI GDGCHC 1 cut(s) 377
MluCI AATT 2 cut(s) 42, 459
MnlI CCTC 4 cut(s) 127, 130, 158, 321
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 2 cut(s) 133, 453
MvaI CCWGG 1 cut(s) 453
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 3 cut(s) 81, 167, 381
NciI CCSGG 1 cut(s) 133
NcoI CCATGG 1 cut(s) 350
NdeII GATC 1 cut(s) 109
NlaIII CATG 2 cut(s) 335, 354
NlaIV GGNNCC 1 cut(s) 137
PfoI TCCNGGA 1 cut(s) 451
PkrI GCNGC 1 cut(s) 260
Ppu21I YACGTR 1 cut(s) 372
PsiI TTATAA 1 cut(s) 11
Psp6I CCWGG 1 cut(s) 451
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 1 cut(s) 137
PspPI GGNCC 1 cut(s) 136
RsaI GTAC 1 cut(s) 156
RsaNI GTAC 1 cut(s) 155
SatI GCNGC 1 cut(s) 259
Sau3AI GATC 1 cut(s) 109
Sau96I GGNCC 1 cut(s) 136
ScrFI CCNGG 2 cut(s) 133, 453
SduI GDGCHC 1 cut(s) 377
SetI ASST 7 cut(s) 25, 172, 210, 231, 253, 374, 406
SfcI CTRYAG 1 cut(s) 126
SinI GGWCC 1 cut(s) 136
SpeI ACTAGT 1 cut(s) 194
Sse9I AATT 2 cut(s) 42, 459
SsiI CCGC 2 cut(s) 96, 318
SspMI CTAG 1 cut(s) 195
StyD4I CCNGG 2 cut(s) 131, 451
StyI CCWWGG 1 cut(s) 350
TaiI ACGT 1 cut(s) 374
TasI AATT 2 cut(s) 42, 459
TatI WGTACW 1 cut(s) 154
TseI GCWGC 1 cut(s) 258
TspDTI ATGAA 1 cut(s) 195
VpaK11BI GGWCC 1 cut(s) 136
XspI CTAG 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.