Rh1DG114900

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0020463
Physical Location & Seq
Forward (+)
21117150 .. 21117927
778 bp
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UTR
Exon/CDS
Intron
Rh1DG114900.1

Sequence Viewer

Length: 300 bp
ATGGCCTTGGCCAGCTATCTCTTCAACACAGAAAATGCTCTGGTTAGGATTGATATGAGTGAGTACATGGAACAGCATGCAGTTTCACGTTTGGTGGGTGCTCCGCCTGGTTATGAAGAAGGTGGACAACTAACAGAAGTGCTGAACCGGGTAAAAGATGGGCTGAAGCAAAAGAAAATCGAGCTTCTTTATACAAAGGAAGCTGTGGAGCTTCTTGGTAAATTGGACTTTGATCTGAAACTTTGGAGCAAGCCCAGTCATCAAGAGGGTAGTACAGACATACACAAATGCATCCCTTAG

Protein Analysis

99

Amino Acids

11.18

Weight (kDa)

5.57

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_2 PF07724 2 - 50 6.3e-12 AAA domain (Cdc48 subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020463)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0330831
rosa_samantha Rh1AG111700 Rh1CG106000 Rh1DG114900 Rh1DG115900 Rh6CG147000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 104
AcoI YGGCCR 1 cut(s) 9
AcuI CTGAAG 1 cut(s) 185
AfaI GTAC 2 cut(s) 65, 274
AgsI TTSAA 1 cut(s) 25
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 4 cut(s) 15, 184, 203, 211
AluI AGCT 4 cut(s) 15, 184, 203, 211
Alw21I GWGCWC 1 cut(s) 103
AoxI GGCC 2 cut(s) 3, 9
AsuC2I CCSGG 1 cut(s) 149
BalI TGGCCA 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 103
BccI CCATC 1 cut(s) 152
BciT130I CCWGG 1 cut(s) 108
BcnI CCSGG 1 cut(s) 149
Bme1390I CCNGG 2 cut(s) 108, 149
BmrFI CCNGG 2 cut(s) 108, 149
BmrI ACTGGG 1 cut(s) 249
BmuI ACTGGG 1 cut(s) 249
BpuMI CCSGG 1 cut(s) 149
BsaJI CCNNGG 1 cut(s) 6
Bse1I ACTGG 1 cut(s) 255
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 1 cut(s) 291
BseNI ACTGG 1 cut(s) 255
BshFI GGCC 2 cut(s) 5, 11
BsiHKAI GWGCWC 1 cut(s) 103
BsiSI CCGG 1 cut(s) 148
BsnI GGCC 2 cut(s) 5, 11
Bsp1286I GDGCHC 1 cut(s) 103
Bsp143I GATC 1 cut(s) 232
BspACI CCGC 1 cut(s) 104
BspANI GGCC 2 cut(s) 5, 11
BsrI ACTGG 1 cut(s) 255
BssECI CCNNGG 1 cut(s) 6
BssMI GATC 1 cut(s) 232
BssT1I CCWWGG 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 108
Bst6I CTCTTC 1 cut(s) 26
BstC8I GCNNGC 3 cut(s) 13, 78, 251
BstDEI CTNAG 1 cut(s) 297
BstF5I GGATG 1 cut(s) 291
BstKTI GATC 1 cut(s) 235
BstMBI GATC 1 cut(s) 232
BstNI CCWGG 1 cut(s) 108
BstNSI RCATGY 1 cut(s) 80
BstSCI CCNGG 2 cut(s) 106, 147
BsuRI GGCC 2 cut(s) 5, 11
BtsCI GGATG 1 cut(s) 291
Cac8I GCNNGC 3 cut(s) 13, 78, 251
Csp6I GTAC 2 cut(s) 64, 273
CviAII CATG 2 cut(s) 67, 77
CviJI RGCY 8 cut(s) 5, 11, 15, 163, 184, 203, 211, 253
CviKI_1 RGCY 8 cut(s) 5, 11, 15, 163, 184, 203, 211, 253
CviQI GTAC 2 cut(s) 64, 273
DdeI CTNAG 1 cut(s) 297
DpnI GATC 1 cut(s) 234
DpnII GATC 1 cut(s) 232
EaeI YGGCCR 1 cut(s) 9
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EciI GGCGGA 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 6
EcoT22I ATGCAT 1 cut(s) 293
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 2 cut(s) 70, 80
FaiI YATR 6 cut(s) 56, 68, 78, 114, 192, 281
FatI CATG 2 cut(s) 66, 76
FokI GGATG 1 cut(s) 278
HaeIII GGCC 2 cut(s) 5, 11
HapII CCGG 1 cut(s) 148
Hin1II CATG 2 cut(s) 70, 80
HpaII CCGG 1 cut(s) 148
Hpy166II GTNNAC 1 cut(s) 125
Hpy188I TCNGA 1 cut(s) 237
Hpy188III TCNNGA 1 cut(s) 263
Hpy8I GTNNAC 1 cut(s) 125
HpyAV CCTTC 1 cut(s) 113
HpyCH4IV ACGT 1 cut(s) 88
HpyCH4V TGCA 2 cut(s) 80, 291
HpyF3I CTNAG 1 cut(s) 297
HpySE526I ACGT 1 cut(s) 88
Hsp92II CATG 2 cut(s) 70, 80
Kzo9I GATC 1 cut(s) 232
LmnI GCTCC 3 cut(s) 106, 208, 246
LpnPI CCDG 6 cut(s) 25, 26, 93, 120, 161, 268
MaeII ACGT 1 cut(s) 88
MalI GATC 1 cut(s) 234
MboI GATC 1 cut(s) 232
MboII GAAGA 2 cut(s) 13, 128
MhlI GDGCHC 1 cut(s) 103
MlsI TGGCCA 1 cut(s) 11
MluCI AATT 1 cut(s) 221
MluNI TGGCCA 1 cut(s) 11
MnlI CCTC 1 cut(s) 259
Mox20I TGGCCA 1 cut(s) 11
Mph1103I ATGCAT 1 cut(s) 293
MscI TGGCCA 1 cut(s) 11
Msp20I TGGCCA 1 cut(s) 11
MspI CCGG 1 cut(s) 148
MspR9I CCNGG 2 cut(s) 108, 149
MvaI CCWGG 1 cut(s) 108
NciI CCSGG 1 cut(s) 149
NdeII GATC 1 cut(s) 232
NlaIII CATG 2 cut(s) 70, 80
NsiI ATGCAT 1 cut(s) 293
NspI RCATGY 1 cut(s) 80
PaeI GCATGC 1 cut(s) 80
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
RsaI GTAC 2 cut(s) 65, 274
RsaNI GTAC 2 cut(s) 64, 273
Sau3AI GATC 1 cut(s) 232
ScrFI CCNGG 2 cut(s) 108, 149
SduI GDGCHC 1 cut(s) 103
SetI ASST 6 cut(s) 17, 91, 124, 186, 205, 213
SphI GCATGC 1 cut(s) 80
Sse9I AATT 1 cut(s) 221
SsiI CCGC 1 cut(s) 104
StyD4I CCNGG 2 cut(s) 106, 147
StyI CCWWGG 1 cut(s) 6
TaiI ACGT 1 cut(s) 91
TaqI TCGA 1 cut(s) 180
TasI AATT 1 cut(s) 221
TatI WGTACW 2 cut(s) 63, 272
TspDTI ATGAA 1 cut(s) 129
XceI RCATGY 1 cut(s) 80
Zsp2I ATGCAT 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.