Rh2AG030000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0025236
Physical Location & Seq
Forward (+)
2093669 .. 2093989
321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG030000.1

Sequence Viewer

Length: 294 bp
ATGGTTCGACGGTATTCCATGGGTGGTTGCGCTCAAAGTAGTCGCAGAAAGCTTCGACTTGTGTTTTCTAGCCGCATATCGACTAACGATGTGGTTGTGCTGATCAAGACCGACAACATGGGTACTACATCGACGGTGGGCAGAGGGTTGGAGTGTGCGGTGGCGCAGTTTGGGTCGAAGGGGCAAGAGATAGGGGTGACATGGGTTGGAGCAGAGCGGGTTGGGAGGAGTCACTGGGCTTGTGGCGTACGACATGTCGTCAGGGGAGGATGGGCTGAGATCAGGCTGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

97

Amino Acids

10.57

Weight (kDa)

11.09

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0025236)

Species Orthologous Gene IDs
rosa_laevigata RLG00000007812
rosa_roxburghii Rroxscaffold_5G00362320
rosa_samantha Rh2AG030000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 217
AciI CCGC 3 cut(s) 73, 158, 217
AfaI GTAC 2 cut(s) 124, 249
AflIII ACRYGT 1 cut(s) 253
AhdI GACNNNNNGTC 1 cut(s) 257
AluBI AGCT 1 cut(s) 52
AluI AGCT 1 cut(s) 52
AspLEI GCGC 2 cut(s) 32, 166
AsuHPI GGTGA 1 cut(s) 208
BccI CCATC 1 cut(s) 264
BclI TGATCA 1 cut(s) 102
BfaI CTAG 1 cut(s) 69
BisI GCNGC 1 cut(s) 73
BlsI GCNGC 1 cut(s) 74
BmeRI GACNNNNNGTC 1 cut(s) 257
BmrI ACTGGG 1 cut(s) 244
BmuI ACTGGG 1 cut(s) 244
BsaJI CCNNGG 1 cut(s) 18
Bse1I ACTGG 1 cut(s) 239
BseDI CCNNGG 1 cut(s) 18
BseGI GGATG 1 cut(s) 275
BseMII CTCAG 1 cut(s) 267
BseNI ACTGG 1 cut(s) 239
BseRI GAGGAG 1 cut(s) 241
BsiWI CGTACG 1 cut(s) 247
Bsp143I GATC 2 cut(s) 102, 279
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 3 cut(s) 73, 158, 217
BspCNI CTCAG 1 cut(s) 268
BsrBI CCGCTC 1 cut(s) 217
BsrI ACTGG 1 cut(s) 239
BssECI CCNNGG 1 cut(s) 18
BssMI GATC 2 cut(s) 102, 279
BssT1I CCWWGG 1 cut(s) 18
Bst4CI ACNGT 2 cut(s) 12, 136
BstC8I GCNNGC 1 cut(s) 288
BstDEI CTNAG 1 cut(s) 276
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 1 cut(s) 275
BstHHI GCGC 2 cut(s) 32, 166
BstKTI GATC 2 cut(s) 105, 282
BstMBI GATC 2 cut(s) 102, 279
BstNSI RCATGY 1 cut(s) 257
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 1 cut(s) 275
BtsIMutI CAGTG 1 cut(s) 232
Cac8I GCNNGC 1 cut(s) 288
CfoI GCGC 2 cut(s) 32, 166
Csp6I GTAC 2 cut(s) 123, 248
CviAII CATG 4 cut(s) 19, 118, 201, 254
CviJI RGCY 6 cut(s) 52, 72, 239, 275, 286, 290
CviKI_1 RGCY 6 cut(s) 52, 72, 239, 275, 286, 290
CviQI GTAC 2 cut(s) 123, 248
DdeI CTNAG 1 cut(s) 276
DpnI GATC 2 cut(s) 104, 281
DpnII GATC 2 cut(s) 102, 279
DriI GACNNNNNGTC 1 cut(s) 257
Eam1105I GACNNNNNGTC 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 18
EcoT14I CCWWGG 1 cut(s) 18
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 4 cut(s) 22, 121, 204, 257
FaiI YATR 5 cut(s) 20, 77, 119, 202, 255
FatI CATG 4 cut(s) 18, 117, 200, 253
FauI CCCGC 1 cut(s) 210
FbaI TGATCA 1 cut(s) 102
Fnu4HI GCNGC 1 cut(s) 73
FokI GGATG 1 cut(s) 282
Fsp4HI GCNGC 1 cut(s) 73
FspBI CTAG 1 cut(s) 69
GlaI GCGC 2 cut(s) 31, 165
GluI GCNGC 1 cut(s) 73
HhaI GCGC 2 cut(s) 32, 166
Hin1II CATG 4 cut(s) 22, 121, 204, 257
Hin6I GCGC 2 cut(s) 30, 164
HinP1I GCGC 2 cut(s) 30, 164
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 1 cut(s) 229
HphI GGTGA 1 cut(s) 208
Hpy188III TCNNGA 1 cut(s) 106
Hpy99I CGWCG 2 cut(s) 12, 136
HpyAV CCTTC 1 cut(s) 172
HpyCH4III ACNGT 2 cut(s) 12, 136
HpyF3I CTNAG 1 cut(s) 276
Hsp92II CATG 4 cut(s) 22, 121, 204, 257
HspAI GCGC 2 cut(s) 30, 164
Ksp22I TGATCA 1 cut(s) 102
Kzo9I GATC 2 cut(s) 102, 279
LmnI GCTCC 1 cut(s) 209
LpnPI CCDG 4 cut(s) 220, 247, 268, 272
MaeI CTAG 1 cut(s) 69
MaeIII GTNAC 2 cut(s) 196, 230
MalI GATC 2 cut(s) 104, 281
MbiI CCGCTC 1 cut(s) 217
MboI GATC 2 cut(s) 102, 279
MlyI GAGTC 1 cut(s) 238
MmeI TCCRAC 2 cut(s) 129, 187
MnlI CCTC 3 cut(s) 137, 219, 260
MseI TTAA 1 cut(s) 292
NcoI CCATGG 1 cut(s) 18
NdeII GATC 2 cut(s) 102, 279
NlaIII CATG 4 cut(s) 22, 121, 204, 257
NmuCI GTSAC 2 cut(s) 196, 230
NspI RCATGY 1 cut(s) 257
PciI ACATGT 1 cut(s) 253
Pfl23II CGTACG 1 cut(s) 247
PkrI GCNGC 1 cut(s) 74
PleI GAGTC 1 cut(s) 237
PpsI GAGTC 1 cut(s) 237
PscI ACATGT 1 cut(s) 253
PspLI CGTACG 1 cut(s) 247
RsaI GTAC 2 cut(s) 124, 249
RsaNI GTAC 2 cut(s) 123, 248
SaqAI TTAA 1 cut(s) 292
SatI GCNGC 1 cut(s) 73
Sau3AI GATC 2 cut(s) 102, 279
SchI GAGTC 1 cut(s) 238
SetI ASST 1 cut(s) 54
SsiI CCGC 3 cut(s) 73, 158, 217
SspMI CTAG 1 cut(s) 69
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 2 cut(s) 12, 136
TaqI TCGA 5 cut(s) 7, 55, 80, 131, 176
TaqII GACCGA 1 cut(s) 125
TauI GCSGC 1 cut(s) 75
Tru1I TTAA 1 cut(s) 292
Tru9I TTAA 1 cut(s) 292
TscAI CASTG 1 cut(s) 239
TseFI GTSAC 2 cut(s) 196, 230
Tsp45I GTSAC 2 cut(s) 196, 230
TspRI CASTG 1 cut(s) 239
XceI RCATGY 1 cut(s) 257
XspI CTAG 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.