Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0010513

Physical Location & Seq

Genomic Coordinates Reverse (-)

4261291 .. 4266218

Gene Structure 4928 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG053600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1965 bp

ATGAATGCGGTGGGGAGACAGATGTCGCGATCCAATTCGGCGATGCAGCACCAGCGGCAGTATTCGGAGACCTTCTTCTCCGACGCGTCGTCGAAATGGCTCCAATCCTCCGGCCTATCACAGGATTTTGAGCTGTACGGGTCGAGATTGAACCGGAATAATCAGAGGAGCGGGAATGAGCGGTCGCCAGAGGCATCGGCGACTCCGATTGTGTCGCGGTCGTCGAGCATGAGAAGGCTGGGCGATGATCGAGTTGAGTTTAGCCCTGGATTGCTTGATCTGCATTCCTTTGATACCGAGCTCTTACCTGAGATGCATGTTCGTGGTAAAAGTTTCGATGACTCTGATCCATTTGTGTCGACCAACAAACTTACAAGCAGATCACGAGGTCTGCCTGAGAATAATCTTCTCAAGAGTGTGTCGATCGATAAAGAAAGGGCCAACAGTGTTGCCAAGATCAAAGTTGTGGTGCGCAAGAGGCCACTAAATAAAAAGGAAATTGCGAAGAAAGAGGAAGACATCATTACAATAGAACCAAATTCTAATGCTCTCACAGTTCATGAAACAAAACTTAAGGTTGACTTAACAGCATATGAGGAGAAGCATGAATTTGTTTTTGATGCCGTTCTGGATGAAGATGTTTCAAATGATGAAGTATACTTTGAAAGTGTGGAACCCATAGTTCCGCTGATTTTCCAACGAACAAAAGCAACTTGCTTTGCATATGGGCAAACAGGTAGTGGAAAGACTTATACCATGCAACCGCTGCCCATTAAAGCAGCCCAAGATATCTTAAGACTAATGCTGAATTCCTATCGAAACCAAGGCTTTCAGTTATTCGTGAGTTTCTTCGAGATATATGGAGGAAAACTTTTTGATCTCCTAAATGATCGGAAGAAACTTTTCATGAGGGAGGATGGTAAACAGCAAGTGTGTATCGTTGGGTTGCAAGAGTACAGGGTGTCAAATGTTGAGACAGTCAAGGAGTTGATTGAGAAGGGAAATTCCACAAGAAGTACTGGTACAACTGGTGCAAATGAAGAATCTTCCCGATCACATGCGATACTTCAGCTTTGTATCAAGAGATCAGCGGATGGAAATGAATCAAAGCCTGCCAGACTTGTAGGAAAGCTGTCTTTCATAGACCTTGCTGGAAGTGAACGCGGTGCAGATACAACAGATAATGATAAACAAACAAGAATGGAAGGTGCTGAAATTAATAAAAGCTTACTTGCTCTAAAAGAATGCATTAGAGCTCTGGACAACGACCAGGGTCACATTCCTTTCAGAGGGAGTAAACTCACCGAAGTTCTTAGAGATTCATTTGTAGGCGACTCACGCACAGTTATGATATCATGCATTTCACCAAGCACAGGATCATGTGAACACACTCTTAACACACTAAGATATGCTGACAGGGTGAAGAGTTTGTCGAAAGGGAGCAGCTCCAGAAAGCAACAACTATCTTCATCTTCAAACCTTAGAGAGTCAACAACATTGCCTTTGTCTTCATCTTTATCAACTGATACAACCTCTGAGGATAACACGCTATCTGTTCCTCAAGAAAAGAGCAGCTTGGGTTGGTCCAAACAGATTGACCGAGAACCCTCCCCCCAGTACGTTGTGAATCGCTTACCCAGTGGGAGGGCAGAAGGACGTTTGCCTGCATCTGTATATTCAGATTCTTACAAGGATCAGATAGGTGGTCAAAGTACCATAGGCGAAGATGACTTTGACTACTCTGATCAGATTTATGAGAAAGAGAGACCATCGTGGCCAAATAGTAAGAAGGTACAAAGCTACCAGACATCAGCTGTGGAGGAAAAGAGAAAGACTGGCAGTCAGGTGAAATGGAGGGATCAGCCTGATTCTGAGGCTATCAACTCTAATTCCGATGATGATCTAATTGCTGTATTGCAGGTAAGAAATAAGATTTCAAATATTGAGTTTAGTACAAATCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005813 GO:0005815 GO:0005856 GO:0005871 GO:0005875 GO:0005886 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0008150 GO:0009987 GO:0015630 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0065007 GO:0071840 GO:0071944 GO:1902850 GO:1903047 GO:1903338

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

654

Amino Acids

73.25

Weight (kDa)

6.4

Isoelectric Point (pI)

51.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	152 - 295	6.8e-14	Microtubule binding
Kinesin	PF00225	158 - 476	1.4e-99	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0010513)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g04820 FvH4_1g04820 FvH4_1g04820 FvH4_1g04820 FvH4_1g04820 FvH4_1g04820
malus_domestica	MD02G1047000.v1.1 MD15G1185900.v1.1
prunus_persica	Prupe.7G231000_v2.0.a1
pyrus_communis	pycom02g03930
rosa_chinensis	RchiOBHm_Chr2g0090451
rosa_laevigata	RLG00000016111
rosa_multiflora	Rmu_sc0004514.1_g000020
rosa_roxburghii	Rroxscaffold_2G00151120
rosa_rugosa	Rorug02G0008100 Rorug02G0008100
rosa_samantha	Rh2AG053600 Rh2BG053000 Rh2CG055000 Rh2DG053800
rosa_wichuraiana	Rw2G004340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	473
Acc36I	ACCTGC	1 cut(s)	1909
AccBSI	CCGCTC	2 cut(s)	171, 181
AccI	GTMKAC	2 cut(s)	359, 657
AccII	CGCG	4 cut(s)	28, 86, 217, 1164
AciI	CCGC	9 cut(s)	8, 55, 171, 181, 217, 686, 764, 1091, 1164
AclWI	GGATC	5 cut(s)	24, 341, 1384, 1701, 1866
AcoI	YGGCCR	1 cut(s)	1775
AcsI	RAATTY	4 cut(s)	538, 608, 808, 1003
AcuI	CTGAAG	1 cut(s)	1052
AfaI	GTAC	8 cut(s)	137, 956, 1018, 1024, 1619, 1714, 1794, 1954
AfiI	CCNNNNNNNGG	4 cut(s)	121, 1289, 1373, 1644
AflII	CTTAAG	2 cut(s)	572, 793
AflIII	ACRYGT	1 cut(s)	84
AgsI	TTSAA	5 cut(s)	151, 645, 665, 1476, 1938
AhdI	GACNNNNNGTC	2 cut(s)	88, 1839
AjnI	CCWGG	2 cut(s)	265, 1269
Alw21I	GWGCWC	2 cut(s)	303, 1258
Alw26I	GTCTC	4 cut(s)	10, 62, 968, 1759
AlwI	GGATC	5 cut(s)	24, 341, 1384, 1701, 1866
AoxI	GGCC	4 cut(s)	112, 438, 479, 1775
ApeKI	GCWGC	5 cut(s)	46, 766, 779, 1443, 1572
ApoI	RAATTY	4 cut(s)	538, 608, 808, 1003
AseI	ATTAAT	1 cut(s)	1218
Asp700I	GAANNNNTTC	1 cut(s)	902
AspLEI	GCGC	1 cut(s)	474
AspS9I	GGNCC	2 cut(s)	438, 1584
AsuHPI	GGTGA	4 cut(s)	1294, 1356, 1432, 1858
AvaII	GGWCC	1 cut(s)	1584
BalI	TGGCCA	1 cut(s)	1777
BanII	GRGCYC	2 cut(s)	303, 1258
BarI	GAAGNNNNNNTAC	2 cut(s)	1006, 1038
BauI	CACGAG	1 cut(s)	384
BbsI	GAAGAC	2 cut(s)	522, 1500
Bbv12I	GWGCWC	2 cut(s)	303, 1258
BbvI	GCAGC	5 cut(s)	58, 753, 791, 1455, 1584
BccI	CCATC	3 cut(s)	911, 1088, 1777
BceAI	ACGGC	1 cut(s)	608
BciT130I	CCWGG	2 cut(s)	267, 1271
BclI	TGATCA	1 cut(s)	1744
BcoDI	GTCTC	4 cut(s)	10, 62, 968, 1759
BfrI	CTTAAG	2 cut(s)	572, 793
BfuAI	ACCTGC	1 cut(s)	1909
BisI	GCNGC	6 cut(s)	47, 56, 767, 780, 1444, 1573
BlsI	GCNGC	6 cut(s)	48, 57, 768, 781, 1445, 1574
BmcAI	AGTACT	1 cut(s)	1018
Bme1390I	CCNGG	2 cut(s)	267, 1271
Bme18I	GGWCC	1 cut(s)	1584
BmeRI	GACNNNNNGTC	2 cut(s)	88, 1839
BmgT120I	GGNCC	2 cut(s)	438, 1584
BmiI	GGNNCC	2 cut(s)	101, 675
BmrFI	CCNGG	2 cut(s)	267, 1271
BmrI	ACTGGG	2 cut(s)	1609, 1632
BmsI	GCATC	5 cut(s)	33, 203, 303, 610, 1676
BmuI	ACTGGG	2 cut(s)	1609, 1632
BoxI	GACNNNNGTC	2 cut(s)	22, 1272
BpiI	GAAGAC	2 cut(s)	522, 1500
BpmI	CTGGAG	1 cut(s)	1432
BpuEI	CTTGAG	2 cut(s)	395, 1545
Bsa29I	ATCGAT	1 cut(s)	426
BsaBI	GATNNNNATC	1 cut(s)	1899
BsaI	GGTCTC	2 cut(s)	62, 1759
BsaJI	CCNNGG	3 cut(s)	265, 823, 1270
BsaWI	WCCGGW	1 cut(s)	153
Bsc4I	CCNNNNNNNGG	4 cut(s)	121, 1289, 1373, 1644
Bse1I	ACTGG	5 cut(s)	1024, 1033, 1615, 1638, 1840
Bse3DI	GCAATG	1 cut(s)	1496
Bse8I	GATNNNNATC	1 cut(s)	1899
BseBI	CCWGG	2 cut(s)	267, 1271
BseCI	ATCGAT	1 cut(s)	426
BseDI	CCNNGG	3 cut(s)	265, 823, 1270
BseGI	GGATG	3 cut(s)	637, 922, 1099
BseJI	GATNNNNATC	1 cut(s)	1899
BseLI	CCNNNNNNNGG	4 cut(s)	121, 1289, 1373, 1644
BseMI	GCAATG	1 cut(s)	1496
BseMII	CTCAG	4 cut(s)	300, 387, 1527, 1863
BseNI	ACTGG	5 cut(s)	1024, 1033, 1615, 1638, 1840
BseRI	GAGGAG	2 cut(s)	181, 611
BseXI	GCAGC	5 cut(s)	58, 753, 791, 1455, 1584
BseYI	CCCAGC	1 cut(s)	238
BsgI	GTGCAG	1 cut(s)	1188
Bsh1236I	CGCG	4 cut(s)	28, 86, 217, 1164
Bsh1285I	CGRYCG	3 cut(s)	185, 221, 426
BshFI	GGCC	4 cut(s)	114, 440, 481, 1777
BshVI	ATCGAT	1 cut(s)	426
BsiEI	CGRYCG	3 cut(s)	185, 221, 426
BsiHKAI	GWGCWC	2 cut(s)	303, 1258
BsiSI	CCGG	2 cut(s)	111, 154
BslI	CCNNNNNNNGG	4 cut(s)	121, 1289, 1373, 1644
BsmAI	GTCTC	4 cut(s)	10, 62, 968, 1759
BsmI	GAATGC	3 cut(s)	10, 283, 1250
BsnI	GGCC	4 cut(s)	114, 440, 481, 1777
Bso31I	GGTCTC	2 cut(s)	62, 1759
Bsp1286I	GDGCHC	2 cut(s)	303, 1258
Bsp68I	TCGCGA	1 cut(s)	28
BspACI	CCGC	9 cut(s)	8, 55, 171, 181, 217, 686, 764, 1091, 1164
BspANI	GGCC	4 cut(s)	114, 440, 481, 1777
BspCNI	CTCAG	4 cut(s)	301, 388, 1528, 1864
BspDI	ATCGAT	1 cut(s)	426
BspFNI	CGCG	4 cut(s)	28, 86, 217, 1164
BspHI	TCATGA	2 cut(s)	559, 906
BspLI	GGNNCC	2 cut(s)	101, 675
BspMI	ACCTGC	1 cut(s)	1909
BspPI	GGATC	5 cut(s)	24, 341, 1384, 1701, 1866
BspTI	CTTAAG	2 cut(s)	572, 793
BspTNI	GGTCTC	2 cut(s)	62, 1759
BsrBI	CCGCTC	2 cut(s)	171, 181
BsrDI	GCAATG	1 cut(s)	1496
BsrI	ACTGG	5 cut(s)	1024, 1033, 1615, 1638, 1840
BssECI	CCNNGG	3 cut(s)	265, 823, 1270
BssNAI	GTATAC	1 cut(s)	658
BssSI	CACGAG	1 cut(s)	384
BssT1I	CCWWGG	1 cut(s)	823
Bst1107I	GTATAC	1 cut(s)	658
Bst2BI	CACGAG	1 cut(s)	384
Bst2UI	CCWGG	2 cut(s)	267, 1271
Bst4CI	ACNGT	4 cut(s)	446, 556, 979, 1345
Bst6I	CTCTTC	1 cut(s)	1418
BstAFI	CTTAAG	2 cut(s)	572, 793
BstAPI	GCANNNNNTGC	1 cut(s)	766
BstC8I	GCNNGC	2 cut(s)	1113, 1665
BstDEI	CTNAG	7 cut(s)	309, 396, 1313, 1403, 1481, 1536, 1872
BstENI	CCTNNNNNAGG	2 cut(s)	119, 1287
BstF5I	GGATG	3 cut(s)	637, 922, 1099
BstFNI	CGCG	4 cut(s)	28, 86, 217, 1164
BstHHI	GCGC	1 cut(s)	474
BstMAI	GTCTC	4 cut(s)	10, 62, 968, 1759
BstMCI	CGRYCG	3 cut(s)	185, 221, 426
BstMWI	GCNNNNNNNGC	6 cut(s)	52, 55, 280, 478, 766, 1338
BstNI	CCWGG	2 cut(s)	267, 1271
BstNSI	RCATGY	2 cut(s)	320, 1061
BstPAI	GACNNNNGTC	2 cut(s)	22, 1272
BstSCI	CCNGG	2 cut(s)	265, 1269
BstUI	CGCG	4 cut(s)	28, 86, 217, 1164
BstV1I	GCAGC	5 cut(s)	58, 753, 791, 1455, 1584
BstV2I	GAAGAC	2 cut(s)	522, 1500
BstZ17I	GTATAC	1 cut(s)	658
Bsu15I	ATCGAT	1 cut(s)	426
BsuRI	GGCC	4 cut(s)	114, 440, 481, 1777
BsuTUI	ATCGAT	1 cut(s)	426
BtgZI	GCGATG	2 cut(s)	56, 258
BtsCI	GGATG	3 cut(s)	637, 922, 1099
BtsIMutI	CAGTG	2 cut(s)	451, 1645
BtuMI	TCGCGA	1 cut(s)	28
BveI	ACCTGC	1 cut(s)	1909
Cac8I	GCNNGC	2 cut(s)	1113, 1665
CciI	TCATGA	2 cut(s)	559, 906
CfoI	GCGC	1 cut(s)	474
Cfr13I	GGNCC	2 cut(s)	438, 1584
ClaI	ATCGAT	1 cut(s)	426
CseI	GACGC	2 cut(s)	75, 92
Csp6I	GTAC	8 cut(s)	136, 955, 1017, 1023, 1618, 1713, 1793, 1953
CviAII	CATG	9 cut(s)	229, 317, 560, 605, 757, 907, 1058, 1356, 1380
CviQI	GTAC	8 cut(s)	136, 955, 1017, 1023, 1618, 1713, 1793, 1953
DdeI	CTNAG	7 cut(s)	309, 396, 1313, 1403, 1481, 1536, 1872
DriI	GACNNNNNGTC	2 cut(s)	88, 1839
EaeI	YGGCCR	1 cut(s)	1775
Eam1104I	CTCTTC	1 cut(s)	1418
Eam1105I	GACNNNNNGTC	2 cut(s)	88, 1839
EarI	CTCTTC	1 cut(s)	1418
Ecl136II	GAGCTC	2 cut(s)	301, 1256
Eco130I	CCWWGG	1 cut(s)	823
Eco24I	GRGCYC	2 cut(s)	303, 1258
Eco31I	GGTCTC	2 cut(s)	62, 1759
Eco32I	GATATC	2 cut(s)	790, 1353
Eco47I	GGWCC	1 cut(s)	1584
Eco53kI	GAGCTC	2 cut(s)	301, 1256
Eco57I	CTGAAG	1 cut(s)	1052
EcoICRI	GAGCTC	2 cut(s)	301, 1256
EcoNI	CCTNNNNNAGG	2 cut(s)	119, 1287
EcoRI	GAATTC	1 cut(s)	808
EcoRII	CCWGG	2 cut(s)	265, 1269
EcoRV	GATATC	2 cut(s)	790, 1353
EcoT14I	CCWWGG	1 cut(s)	823
EcoT22I	ATGCAT	3 cut(s)	318, 1250, 1361
EcoT38I	GRGCYC	2 cut(s)	303, 1258
ErhI	CCWWGG	1 cut(s)	823
FaeI	CATG	9 cut(s)	232, 320, 563, 608, 760, 910, 1061, 1359, 1383
FalI	AAGNNNNNCTT	4 cut(s)	566, 598, 1559, 1591
FatI	CATG	9 cut(s)	228, 316, 559, 604, 756, 906, 1057, 1355, 1379
FauI	CCCGC	1 cut(s)	164
FauNDI	CATATG	2 cut(s)	592, 724
FbaI	TGATCA	1 cut(s)	1744
FblI	GTMKAC	2 cut(s)	359, 657
Fnu4HI	GCNGC	6 cut(s)	47, 56, 767, 780, 1444, 1573
FokI	GGATG	3 cut(s)	644, 929, 1106
FriOI	GRGCYC	2 cut(s)	303, 1258
Fsp4HI	GCNGC	6 cut(s)	47, 56, 767, 780, 1444, 1573
FspI	TGCGCA	1 cut(s)	473
GlaI	GCGC	1 cut(s)	473
GluI	GCNGC	6 cut(s)	47, 56, 767, 780, 1444, 1573
GsaI	CCCAGC	1 cut(s)	242
GsuI	CTGGAG	1 cut(s)	1432
HaeIII	GGCC	4 cut(s)	114, 440, 481, 1777
HapII	CCGG	2 cut(s)	111, 154
HgaI	GACGC	2 cut(s)	75, 92
HhaI	GCGC	1 cut(s)	474
Hin1II	CATG	9 cut(s)	232, 320, 563, 608, 760, 910, 1061, 1359, 1383
Hin6I	GCGC	1 cut(s)	472
HinP1I	GCGC	1 cut(s)	472
HincII	GTYRAC	3 cut(s)	360, 580, 1491
HindII	GTYRAC	3 cut(s)	360, 580, 1491
HindIII	AAGCTT	1 cut(s)	1225
HpaII	CCGG	2 cut(s)	111, 154
HphI	GGTGA	4 cut(s)	1294, 1356, 1432, 1858
Hpy166II	GTNNAC	8 cut(s)	360, 580, 658, 923, 1160, 1298, 1385, 1491
Hpy8I	GTNNAC	8 cut(s)	360, 580, 658, 923, 1160, 1298, 1385, 1491
Hpy99I	CGWCG	4 cut(s)	86, 91, 94, 226
HpyAV	CCTTC	6 cut(s)	82, 228, 991, 1199, 1646, 1783
HpyCH4III	ACNGT	4 cut(s)	446, 556, 979, 1345
HpyCH4IV	ACGT	2 cut(s)	1620, 1657
HpyF10VI	GCNNNNNNNGC	6 cut(s)	52, 55, 280, 478, 766, 1338
HpyF3I	CTNAG	7 cut(s)	309, 396, 1313, 1403, 1481, 1536, 1872
HpySE526I	ACGT	2 cut(s)	1620, 1657
Hsp92II	CATG	9 cut(s)	232, 320, 563, 608, 760, 910, 1061, 1359, 1383
HspAI	GCGC	1 cut(s)	472
Ksp22I	TGATCA	1 cut(s)	1744
LmnI	GCTCC	4 cut(s)	105, 168, 1440, 1451
Lsp1109I	GCAGC	5 cut(s)	58, 753, 791, 1455, 1584
LweI	GCATC	5 cut(s)	33, 203, 303, 610, 1676
MaeII	ACGT	2 cut(s)	1620, 1657
MaeIII	GTNAC	1 cut(s)	1274
MbiI	CCGCTC	2 cut(s)	171, 181
MhlI	GDGCHC	2 cut(s)	303, 1258
MlsI	TGGCCA	1 cut(s)	1777
MluCI	AATT	9 cut(s)	34, 498, 538, 608, 808, 1003, 1215, 1888, 1905
MluI	ACGCGT	1 cut(s)	84
MluNI	TGGCCA	1 cut(s)	1777
MlyI	GAGTC	4 cut(s)	196, 335, 1328, 1496
MmeI	TCCRAC	2 cut(s)	105, 721
Mox20I	TGGCCA	1 cut(s)	1777
Mph1103I	ATGCAT	3 cut(s)	318, 1250, 1361
MroXI	GAANNNNTTC	1 cut(s)	902
MscI	TGGCCA	1 cut(s)	1777
MseI	TTAA	6 cut(s)	573, 584, 774, 794, 1218, 1395
MslI	CAYNNNNRTG	2 cut(s)	321, 1346
Msp20I	TGGCCA	1 cut(s)	1777
MspA1I	CMGCKG	5 cut(s)	55, 688, 766, 1091, 1814
MspCI	CTTAAG	2 cut(s)	572, 793
MspI	CCGG	2 cut(s)	111, 154
MspR9I	CCNGG	2 cut(s)	267, 1271
Mva1269I	GAATGC	3 cut(s)	10, 283, 1250
MvaI	CCWGG	2 cut(s)	267, 1271
MvnI	CGCG	4 cut(s)	28, 86, 217, 1164
MwoI	GCNNNNNNNGC	6 cut(s)	52, 55, 280, 478, 766, 1338
NdeI	CATATG	2 cut(s)	592, 724
NlaIII	CATG	9 cut(s)	232, 320, 563, 608, 760, 910, 1061, 1359, 1383
NlaIV	GGNNCC	2 cut(s)	101, 675
NmuCI	GTSAC	1 cut(s)	1274
NruI	TCGCGA	1 cut(s)	28
NsbI	TGCGCA	1 cut(s)	473
NsiI	ATGCAT	3 cut(s)	318, 1250, 1361
NspI	RCATGY	2 cut(s)	320, 1061
PagI	TCATGA	2 cut(s)	559, 906
PcsI	WCGNNNNNNNCGW	2 cut(s)	203, 221
PctI	GAATGC	3 cut(s)	10, 283, 1250
PdmI	GAANNNNTTC	1 cut(s)	902
PfeI	GAWTC	6 cut(s)	1043, 1103, 1319, 1627, 1682, 1868
PkrI	GCNGC	6 cut(s)	48, 57, 768, 781, 1445, 1574
Ple19I	CGATCG	1 cut(s)	426
PleI	GAGTC	4 cut(s)	196, 335, 1328, 1495
PpsI	GAGTC	4 cut(s)	196, 335, 1328, 1495
PshAI	GACNNNNGTC	2 cut(s)	22, 1272
PshBI	ATTAAT	1 cut(s)	1218
Psp124BI	GAGCTC	2 cut(s)	303, 1258
Psp6I	CCWGG	2 cut(s)	265, 1269
PspFI	CCCAGC	1 cut(s)	238
PspGI	CCWGG	2 cut(s)	265, 1269
PspN4I	GGNNCC	2 cut(s)	101, 675
PspPI	GGNCC	2 cut(s)	438, 1584
PvuI	CGATCG	1 cut(s)	426
PvuII	CAGCTG	1 cut(s)	1814
RruI	TCGCGA	1 cut(s)	28
RsaI	GTAC	8 cut(s)	137, 956, 1018, 1024, 1619, 1714, 1794, 1954
RsaNI	GTAC	8 cut(s)	136, 955, 1017, 1023, 1618, 1713, 1793, 1953
RseI	CAYNNNNRTG	2 cut(s)	321, 1346
SacI	GAGCTC	2 cut(s)	303, 1258
SalI	GTCGAC	1 cut(s)	358
SaqAI	TTAA	6 cut(s)	573, 584, 774, 794, 1218, 1395
SatI	GCNGC	6 cut(s)	47, 56, 767, 780, 1444, 1573
Sau96I	GGNCC	2 cut(s)	438, 1584
ScaI	AGTACT	1 cut(s)	1018
SchI	GAGTC	4 cut(s)	196, 335, 1328, 1496
ScrFI	CCNGG	2 cut(s)	267, 1271
SduI	GDGCHC	2 cut(s)	303, 1258
SfaNI	GCATC	5 cut(s)	33, 203, 303, 610, 1676
SinI	GGWCC	1 cut(s)	1584
SmiMI	CAYNNNNRTG	2 cut(s)	321, 1346
SmlI	CTYRAG	4 cut(s)	410, 572, 793, 1560
SmoI	CTYRAG	4 cut(s)	410, 572, 793, 1560
Sse9I	AATT	9 cut(s)	34, 498, 538, 608, 808, 1003, 1215, 1888, 1905
SsiI	CCGC	9 cut(s)	8, 55, 171, 181, 217, 686, 764, 1091, 1164
SspI	AATATT	1 cut(s)	1942
SstI	GAGCTC	2 cut(s)	303, 1258
StyD4I	CCNGG	2 cut(s)	265, 1269
StyI	CCWWGG	1 cut(s)	823
TaaI	ACNGT	4 cut(s)	446, 556, 979, 1345
TaiI	ACGT	2 cut(s)	1623, 1660
TaqII	GACCGA	1 cut(s)	1614
TasI	AATT	9 cut(s)	34, 498, 538, 608, 808, 1003, 1215, 1888, 1905
TatI	WGTACW	3 cut(s)	954, 1016, 1952
TauI	GCSGC	1 cut(s)	58
TfiI	GAWTC	6 cut(s)	1043, 1103, 1319, 1627, 1682, 1868
Tru1I	TTAA	6 cut(s)	573, 584, 774, 794, 1218, 1395
Tru9I	TTAA	6 cut(s)	573, 584, 774, 794, 1218, 1395
TscAI	CASTG	2 cut(s)	451, 1645
TseFI	GTSAC	1 cut(s)	1274
TseI	GCWGC	5 cut(s)	46, 766, 779, 1443, 1572
Tsp45I	GTSAC	1 cut(s)	1274
TspRI	CASTG	2 cut(s)	451, 1645
Vha464I	CTTAAG	2 cut(s)	572, 793
VpaK11BI	GGWCC	1 cut(s)	1584
VspI	ATTAAT	1 cut(s)	1218
XagI	CCTNNNNNAGG	2 cut(s)	119, 1287
XapI	RAATTY	4 cut(s)	538, 608, 808, 1003
XceI	RCATGY	2 cut(s)	320, 1061
XmiI	GTMKAC	2 cut(s)	359, 657
XmnI	GAANNNNTTC	1 cut(s)	902
ZrmI	AGTACT	1 cut(s)	1018
Zsp2I	ATGCAT	3 cut(s)	318, 1250, 1361

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.