Rh2BG152500

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0023121
Physical Location & Seq
Forward (+)
13101657 .. 13103978
2322 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG152500.1

Sequence Viewer

Length: 906 bp
ATGGCGAAACGGCAAACTGTTTTCTCAAGTCCTGGGTTGCTATTTTTCGTGGGTATCGTAACAAAATCATACATCCTAATCTCTCTCAAGCTGCATTACTTTTTCCATCGAGGGATGAGTGATGACTTGCCTGAAAAGTATGTAGCTATGATGGAAAGGAGGTGGTTTGGCGAGAAAAAAGTTGTTACAAGGAGATTTGTATTTGATGCGAAAGCCATATCCACACTTCAAGATGAAGGGAAGAGCGAGTACGTGCCCAAACGATCACGTGTTCAGGCCCTCACTGGTTTTCTCTGGAAACATCAACTCGCTGCTAATTCTCGGGCATTATCATCAGGTACTTCAACAAGATTTTCCGTAGCATCTCAGACAGTGAACTTAAGGTCAAAAATGAACATGAAAACGACGTTGGACAATGCCATTGGTAATATCTTTTTGTGGGCTTCGGCACAGCTAGATCTAAATGATACAGCACCAGGGAGCAGTGATCTTAAGTTGTGTAACTTGGTTAACTTACTCAATGAATCTATCAAAGAATTTAACAGTGATTACTTGGAGACTTTGAAGGGTAAAGAGGGATATGGAGGCATGTGTGATTTGCTAGATTTCATGGAAGAGGGGAGTTCTGTAGAACCAGCACCAGAGTTTTATTCATTCTCAAGCTGGACTAGATTTTTTGACCAAGTTGATTTTGGATGGGGGAGGCCATCTTGGGTTGGAGTCTCGGGGAGAGTTGGAAATAGAAATTTCACAACATTCGTTGAAACACAATGCGATGACGGAATTGAAGCGTGGGTGACTCTAGATGAAAAACAAATGGCTATGCTAGAACAAGATCCACAGTTTTTAGCATTTGCATCTCCAAACCCCCGAATTTCAATAACCTCTTCAGTTGGTATTGATTAA

Protein Analysis

301

Amino Acids

33.94

Weight (kDa)

6.02

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 58 - 282 1.2e-23 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023121)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0006153.1_g000053
rosa_samantha Rh2AG147300 Rh2BG152500 Rh2DG152700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 830
AcsI RAATTY 3 cut(s) 536, 745, 873
AcuI CTGAAG 1 cut(s) 873
AcvI CACGTG 1 cut(s) 269
AfaI GTAC 2 cut(s) 251, 340
AflII CTTAAG 2 cut(s) 379, 491
AflIII ACRYGT 1 cut(s) 268
AgsI TTSAA 6 cut(s) 230, 345, 565, 764, 788, 879
AjnI CCWGG 2 cut(s) 31, 475
AjuI GAANNNNNNNTTGG 2 cut(s) 392, 424
AluBI AGCT 4 cut(s) 91, 146, 454, 663
AluI AGCT 4 cut(s) 91, 146, 454, 663
Alw26I GTCTC 2 cut(s) 551, 727
AlwI GGATC 1 cut(s) 830
Ama87I CYCGRG 2 cut(s) 321, 724
AoxI GGCC 2 cut(s) 276, 704
ApeKI GCWGC 2 cut(s) 91, 311
ApoI RAATTY 3 cut(s) 536, 745, 873
AspS9I GGNCC 1 cut(s) 277
AsuHPI GGTGA 1 cut(s) 808
AvaI CYCGRG 2 cut(s) 321, 724
BaeGI GKGCMC 1 cut(s) 258
BarI GAAGNNNNNNTAC 2 cut(s) 233, 265
BbrPI CACGTG 1 cut(s) 269
BbvI GCAGC 2 cut(s) 78, 298
BccI CCATC 4 cut(s) 114, 145, 690, 715
BceAI ACGGC 1 cut(s) 26
BciT130I CCWGG 2 cut(s) 33, 477
BcoDI GTCTC 2 cut(s) 551, 727
BfaI CTAG 5 cut(s) 455, 602, 669, 803, 827
BfmI CTRYAG 1 cut(s) 627
BfrI CTTAAG 2 cut(s) 379, 491
BglII AGATCT 1 cut(s) 457
BisI GCNGC 2 cut(s) 92, 312
BlsI GCNGC 2 cut(s) 93, 313
Bme1390I CCNGG 2 cut(s) 33, 477
BmeT110I CYCGRG 2 cut(s) 321, 724
BmgT120I GGNCC 1 cut(s) 277
BmrFI CCNGG 2 cut(s) 33, 477
BmsI GCATC 3 cut(s) 196, 371, 866
BpuEI CTTGAG 3 cut(s) 10, 71, 643
BsaAI YACGTR 2 cut(s) 253, 269
BsaJI CCNNGG 2 cut(s) 32, 476
Bse1I ACTGG 1 cut(s) 289
BseBI CCWGG 2 cut(s) 33, 477
BseDI CCNNGG 2 cut(s) 32, 476
BseGI GGATG 3 cut(s) 72, 120, 701
BseMII CTCAG 1 cut(s) 380
BseNI ACTGG 1 cut(s) 289
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 2 cut(s) 78, 298
BshFI GGCC 2 cut(s) 278, 706
BsiHKCI CYCGRG 2 cut(s) 321, 724
BsmAI GTCTC 2 cut(s) 551, 727
BsnI GGCC 2 cut(s) 278, 706
BsoBI CYCGRG 2 cut(s) 321, 724
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 4 cut(s) 263, 457, 487, 835
BspANI GGCC 2 cut(s) 278, 706
BspCNI CTCAG 1 cut(s) 379
BspPI GGATC 1 cut(s) 830
BspQI GCTCTTC 1 cut(s) 236
BspTI CTTAAG 2 cut(s) 379, 491
BsrI ACTGG 1 cut(s) 289
BssECI CCNNGG 2 cut(s) 32, 476
BssMI GATC 4 cut(s) 263, 457, 487, 835
Bst2UI CCWGG 2 cut(s) 33, 477
Bst4CI ACNGT 4 cut(s) 19, 373, 545, 843
Bst6I CTCTTC 3 cut(s) 236, 609, 892
BstAFI CTTAAG 2 cut(s) 379, 491
BstBAI YACGTR 2 cut(s) 253, 269
BstDEI CTNAG 1 cut(s) 366
BstF5I GGATG 3 cut(s) 72, 120, 701
BstKTI GATC 4 cut(s) 266, 460, 490, 838
BstMAI GTCTC 2 cut(s) 551, 727
BstMBI GATC 4 cut(s) 263, 457, 487, 835
BstNI CCWGG 2 cut(s) 33, 477
BstNSI RCATGY 1 cut(s) 592
BstSCI CCNGG 2 cut(s) 31, 475
BstSFI CTRYAG 1 cut(s) 627
BstSLI GKGCMC 1 cut(s) 258
BstV1I GCAGC 2 cut(s) 78, 298
BstX2I RGATCY 2 cut(s) 457, 835
BstYI RGATCY 2 cut(s) 457, 835
BsuRI GGCC 2 cut(s) 278, 706
BtgZI GCGATG 1 cut(s) 789
BtsCI GGATG 3 cut(s) 72, 120, 701
BtsI GCAGTG 1 cut(s) 490
BtsIMutI CAGTG 4 cut(s) 282, 378, 490, 550
Cfr13I GGNCC 1 cut(s) 277
Csp6I GTAC 2 cut(s) 250, 339
CviAII CATG 3 cut(s) 397, 589, 610
CviJI RGCY 9 cut(s) 91, 146, 215, 278, 443, 454, 663, 706, 821
CviKI_1 RGCY 9 cut(s) 91, 146, 215, 278, 443, 454, 663, 706, 821
CviQI GTAC 2 cut(s) 250, 339
DdeI CTNAG 1 cut(s) 366
DpnI GATC 4 cut(s) 265, 459, 489, 837
DpnII GATC 4 cut(s) 263, 457, 487, 835
Eam1104I CTCTTC 3 cut(s) 236, 609, 892
EarI CTCTTC 3 cut(s) 236, 609, 892
Eco57I CTGAAG 1 cut(s) 873
Eco72I CACGTG 1 cut(s) 269
Eco88I CYCGRG 2 cut(s) 321, 724
EcoO109I RGGNCCY 1 cut(s) 277
EcoRII CCWGG 2 cut(s) 31, 475
FaeI CATG 3 cut(s) 400, 592, 613
FaiI YATR 9 cut(s) 70, 141, 149, 218, 398, 582, 590, 611, 824
FatI CATG 3 cut(s) 396, 588, 609
Fnu4HI GCNGC 2 cut(s) 92, 312
FokI GGATG 3 cut(s) 59, 127, 708
Fsp4HI GCNGC 2 cut(s) 92, 312
FspBI CTAG 5 cut(s) 455, 602, 669, 803, 827
GluI GCNGC 2 cut(s) 92, 312
HaeIII GGCC 2 cut(s) 278, 706
Hin1II CATG 3 cut(s) 400, 592, 613
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HinfI GANTC 3 cut(s) 524, 720, 799
HpaI GTTAAC 1 cut(s) 511
HphI GGTGA 1 cut(s) 808
Hpy166II GTNNAC 2 cut(s) 376, 511
Hpy188I TCNGA 1 cut(s) 369
Hpy188III TCNNGA 3 cut(s) 230, 295, 803
Hpy8I GTNNAC 2 cut(s) 376, 511
Hpy99I CGWCG 1 cut(s) 409
HpyAV CCTTC 2 cut(s) 230, 559
HpyCH4III ACNGT 4 cut(s) 19, 373, 545, 843
HpyCH4IV ACGT 3 cut(s) 252, 268, 407
HpyCH4V TGCA 2 cut(s) 94, 857
HpyF3I CTNAG 1 cut(s) 366
HpySE526I ACGT 3 cut(s) 252, 268, 407
Hsp92II CATG 3 cut(s) 400, 592, 613
KspAI GTTAAC 1 cut(s) 511
Kzo9I GATC 4 cut(s) 263, 457, 487, 835
LguI GCTCTTC 1 cut(s) 236
LmnI GCTCC 1 cut(s) 480
Lsp1109I GCAGC 2 cut(s) 78, 298
LweI GCATC 3 cut(s) 196, 371, 866
MaeI CTAG 5 cut(s) 455, 602, 669, 803, 827
MaeII ACGT 3 cut(s) 252, 268, 407
MaeIII GTNAC 4 cut(s) 58, 184, 500, 796
MalI GATC 4 cut(s) 265, 459, 489, 837
MboI GATC 4 cut(s) 263, 457, 487, 835
MboII GAAGA 3 cut(s) 253, 626, 879
MflI RGATCY 2 cut(s) 457, 835
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 5 cut(s) 316, 536, 745, 783, 873
MlyI GAGTC 2 cut(s) 729, 793
MmeI TCCRAC 3 cut(s) 390, 697, 715
MnlI CCTC 8 cut(s) 104, 153, 290, 568, 578, 610, 696, 895
MseI TTAA 5 cut(s) 380, 492, 510, 540, 904
MspCI CTTAAG 2 cut(s) 379, 491
MspR9I CCNGG 2 cut(s) 33, 477
MvaI CCWGG 2 cut(s) 33, 477
NdeII GATC 4 cut(s) 263, 457, 487, 835
NlaIII CATG 3 cut(s) 400, 592, 613
NmuCI GTSAC 1 cut(s) 796
NspI RCATGY 1 cut(s) 592
PciSI GCTCTTC 1 cut(s) 236
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PfeI GAWTC 1 cut(s) 524
PkrI GCNGC 2 cut(s) 93, 313
PleI GAGTC 2 cut(s) 728, 793
PmaCI CACGTG 1 cut(s) 269
PmlI CACGTG 1 cut(s) 269
PpsI GAGTC 2 cut(s) 728, 793
Ppu21I YACGTR 2 cut(s) 253, 269
Psp6I CCWGG 2 cut(s) 31, 475
PspCI CACGTG 1 cut(s) 269
PspGI CCWGG 2 cut(s) 31, 475
PspPI GGNCC 1 cut(s) 277
PsuI RGATCY 2 cut(s) 457, 835
RsaI GTAC 2 cut(s) 251, 340
RsaNI GTAC 2 cut(s) 250, 339
SapI GCTCTTC 1 cut(s) 236
SaqAI TTAA 5 cut(s) 380, 492, 510, 540, 904
SatI GCNGC 2 cut(s) 92, 312
Sau3AI GATC 4 cut(s) 263, 457, 487, 835
Sau96I GGNCC 1 cut(s) 277
SchI GAGTC 2 cut(s) 729, 793
ScrFI CCNGG 2 cut(s) 33, 477
SduI GDGCHC 1 cut(s) 258
SfaNI GCATC 3 cut(s) 196, 371, 866
SfcI CTRYAG 1 cut(s) 627
SmlI CTYRAG 5 cut(s) 25, 86, 379, 491, 658
SmoI CTYRAG 5 cut(s) 25, 86, 379, 491, 658
Sse9I AATT 5 cut(s) 316, 536, 745, 783, 873
SspMI CTAG 5 cut(s) 455, 602, 669, 803, 827
StyD4I CCNGG 2 cut(s) 31, 475
TaaI ACNGT 4 cut(s) 19, 373, 545, 843
TaiI ACGT 3 cut(s) 255, 271, 410
TaqI TCGA 1 cut(s) 109
TasI AATT 5 cut(s) 316, 536, 745, 783, 873
TfiI GAWTC 1 cut(s) 524
Tru1I TTAA 5 cut(s) 380, 492, 510, 540, 904
Tru9I TTAA 5 cut(s) 380, 492, 510, 540, 904
TscAI CASTG 4 cut(s) 289, 378, 490, 550
TseFI GTSAC 1 cut(s) 796
TseI GCWGC 2 cut(s) 91, 311
Tsp45I GTSAC 1 cut(s) 796
TspDTI ATGAA 7 cut(s) 249, 407, 413, 537, 598, 642, 822
TspGWI ACGGA 2 cut(s) 346, 795
TspRI CASTG 4 cut(s) 289, 378, 490, 550
Vha464I CTTAAG 2 cut(s) 379, 491
XapI RAATTY 3 cut(s) 536, 745, 873
XbaI TCTAGA 1 cut(s) 802
XceI RCATGY 1 cut(s) 592
XcmI CCANNNNNNNNNTGG 1 cut(s) 689
XspI CTAG 5 cut(s) 455, 602, 669, 803, 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.