Rh2DG152700

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0023121
Physical Location & Seq
Forward (+)
12817762 .. 12819101
1340 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG152700.1

Sequence Viewer

Length: 759 bp
ATGATGGAAAGGAGGTGGTTTGGCGAGAAAAAAGTTGTTACAAGGAGATTTGTATTTGATGCGAAAGCCATATCCACACTTCAAGATGAAGGGAAGAGCGAGTACGTGCCCAAACGATCACGTGTTCAGGCCCTCACTGGTTTTCTCTGGAAACATCAACTCGCTGCTAATTCTCGGGCATTATCATCAGGTACTTCAACAAGATTTTCCGTAGCATCTCAGACAGTGAACTTAAGGTCAAAAATGAACATGAAAACGACGTTGGACAATGCCATTGGTAATATCTTTTTGTGGGCTTCGGCACAGCTAGATCTAAATGATACAGCACCAGGGAGCAGTGATCTTAAGTTGTGTAACTTGGTTAACTTACTCAATGAATCTATCAAAGAATTTAACAGTGATTACTTGGAGACTTTGAAGGGTAAAGAGGGATATGGAGGCATGTGTGATTTGCTAGATTTCATGGAAGAGGGGAGTTCTGTAGAACCAGCACCAGAGTTTTATTCATTCTCAAGCTGGACTAGATTTTTTGACCAAGTTGATTTTGGATGGGGGAGGCCATCTTGGGTTGGAGTCTCGGGGAGAGTTGGAAATAGAAATTTCACAACATTCGTTGAAACACAATGCGATGACGGAATTGAAGCGTGGGTGACTCTAGATGAAAAACAAATGGCTATGCTAGAACAAGATCCACAGTTTTTAGCATTTGCATCTCCAAACCCCCGAATTTCAATAACCTCTTCAGTTGGTATTGATTAA

Protein Analysis

252

Amino Acids

28.3

Weight (kDa)

5.14

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 10 - 233 8.9e-24 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023121)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0006153.1_g000053
rosa_samantha Rh2AG147300 Rh2BG152500 Rh2DG152700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 683
AcsI RAATTY 3 cut(s) 389, 598, 726
AcuI CTGAAG 1 cut(s) 726
AcvI CACGTG 1 cut(s) 122
AfaI GTAC 2 cut(s) 104, 193
AflII CTTAAG 2 cut(s) 232, 344
AflIII ACRYGT 1 cut(s) 121
AgsI TTSAA 6 cut(s) 83, 198, 418, 617, 641, 732
AjnI CCWGG 1 cut(s) 328
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
AluBI AGCT 2 cut(s) 307, 516
AluI AGCT 2 cut(s) 307, 516
Alw26I GTCTC 2 cut(s) 404, 580
AlwI GGATC 1 cut(s) 683
Ama87I CYCGRG 2 cut(s) 174, 577
AoxI GGCC 2 cut(s) 129, 557
ApeKI GCWGC 1 cut(s) 164
ApoI RAATTY 3 cut(s) 389, 598, 726
AspS9I GGNCC 1 cut(s) 130
AsuHPI GGTGA 1 cut(s) 661
AvaI CYCGRG 2 cut(s) 174, 577
BaeGI GKGCMC 1 cut(s) 111
BarI GAAGNNNNNNTAC 2 cut(s) 86, 118
BbrPI CACGTG 1 cut(s) 122
BbvI GCAGC 1 cut(s) 151
BccI CCATC 2 cut(s) 543, 568
BciT130I CCWGG 1 cut(s) 330
BcoDI GTCTC 2 cut(s) 404, 580
BfaI CTAG 5 cut(s) 308, 455, 522, 656, 680
BfmI CTRYAG 1 cut(s) 480
BfrI CTTAAG 2 cut(s) 232, 344
BglII AGATCT 1 cut(s) 310
BisI GCNGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 330
BmeT110I CYCGRG 2 cut(s) 174, 577
BmgT120I GGNCC 1 cut(s) 130
BmrFI CCNGG 1 cut(s) 330
BmsI GCATC 3 cut(s) 49, 224, 719
BpuEI CTTGAG 1 cut(s) 496
BsaAI YACGTR 2 cut(s) 106, 122
BsaJI CCNNGG 1 cut(s) 329
Bse1I ACTGG 1 cut(s) 142
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 1 cut(s) 329
BseGI GGATG 1 cut(s) 554
BseMII CTCAG 1 cut(s) 233
BseNI ACTGG 1 cut(s) 142
BseSI GKGCMC 1 cut(s) 111
BseXI GCAGC 1 cut(s) 151
BshFI GGCC 2 cut(s) 131, 559
BsiHKCI CYCGRG 2 cut(s) 174, 577
BsmAI GTCTC 2 cut(s) 404, 580
BsnI GGCC 2 cut(s) 131, 559
BsoBI CYCGRG 2 cut(s) 174, 577
Bsp1286I GDGCHC 1 cut(s) 111
Bsp143I GATC 4 cut(s) 116, 310, 340, 688
BspANI GGCC 2 cut(s) 131, 559
BspCNI CTCAG 1 cut(s) 232
BspPI GGATC 1 cut(s) 683
BspQI GCTCTTC 1 cut(s) 89
BspTI CTTAAG 2 cut(s) 232, 344
BsrI ACTGG 1 cut(s) 142
BssECI CCNNGG 1 cut(s) 329
BssMI GATC 4 cut(s) 116, 310, 340, 688
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 3 cut(s) 226, 398, 696
Bst6I CTCTTC 3 cut(s) 89, 462, 745
BstAFI CTTAAG 2 cut(s) 232, 344
BstBAI YACGTR 2 cut(s) 106, 122
BstDEI CTNAG 1 cut(s) 219
BstF5I GGATG 1 cut(s) 554
BstKTI GATC 4 cut(s) 119, 313, 343, 691
BstMAI GTCTC 2 cut(s) 404, 580
BstMBI GATC 4 cut(s) 116, 310, 340, 688
BstNI CCWGG 1 cut(s) 330
BstNSI RCATGY 1 cut(s) 445
BstSCI CCNGG 1 cut(s) 328
BstSFI CTRYAG 1 cut(s) 480
BstSLI GKGCMC 1 cut(s) 111
BstV1I GCAGC 1 cut(s) 151
BstX2I RGATCY 2 cut(s) 310, 688
BstYI RGATCY 2 cut(s) 310, 688
BsuRI GGCC 2 cut(s) 131, 559
BtgZI GCGATG 1 cut(s) 642
BtsCI GGATG 1 cut(s) 554
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 4 cut(s) 135, 231, 343, 403
Cfr13I GGNCC 1 cut(s) 130
Csp6I GTAC 2 cut(s) 103, 192
CviAII CATG 3 cut(s) 250, 442, 463
CviJI RGCY 7 cut(s) 68, 131, 296, 307, 516, 559, 674
CviKI_1 RGCY 7 cut(s) 68, 131, 296, 307, 516, 559, 674
CviQI GTAC 2 cut(s) 103, 192
DdeI CTNAG 1 cut(s) 219
DpnI GATC 4 cut(s) 118, 312, 342, 690
DpnII GATC 4 cut(s) 116, 310, 340, 688
Eam1104I CTCTTC 3 cut(s) 89, 462, 745
EarI CTCTTC 3 cut(s) 89, 462, 745
Eco57I CTGAAG 1 cut(s) 726
Eco72I CACGTG 1 cut(s) 122
Eco88I CYCGRG 2 cut(s) 174, 577
EcoO109I RGGNCCY 1 cut(s) 130
EcoRII CCWGG 1 cut(s) 328
FaeI CATG 3 cut(s) 253, 445, 466
FaiI YATR 6 cut(s) 71, 251, 435, 443, 464, 677
FatI CATG 3 cut(s) 249, 441, 462
Fnu4HI GCNGC 1 cut(s) 165
FokI GGATG 1 cut(s) 561
Fsp4HI GCNGC 1 cut(s) 165
FspBI CTAG 5 cut(s) 308, 455, 522, 656, 680
GluI GCNGC 1 cut(s) 165
HaeIII GGCC 2 cut(s) 131, 559
Hin1II CATG 3 cut(s) 253, 445, 466
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 3 cut(s) 377, 573, 652
HpaI GTTAAC 1 cut(s) 364
HphI GGTGA 1 cut(s) 661
Hpy166II GTNNAC 2 cut(s) 229, 364
Hpy188I TCNGA 1 cut(s) 222
Hpy188III TCNNGA 3 cut(s) 83, 148, 656
Hpy8I GTNNAC 2 cut(s) 229, 364
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 2 cut(s) 83, 412
HpyCH4III ACNGT 3 cut(s) 226, 398, 696
HpyCH4IV ACGT 3 cut(s) 105, 121, 260
HpyCH4V TGCA 1 cut(s) 710
HpyF3I CTNAG 1 cut(s) 219
HpySE526I ACGT 3 cut(s) 105, 121, 260
Hsp92II CATG 3 cut(s) 253, 445, 466
KspAI GTTAAC 1 cut(s) 364
Kzo9I GATC 4 cut(s) 116, 310, 340, 688
LguI GCTCTTC 1 cut(s) 89
LmnI GCTCC 1 cut(s) 333
LpnPI CCDG 9 cut(s) 113, 123, 133, 174, 315, 342, 501, 502, 507
Lsp1109I GCAGC 1 cut(s) 151
LweI GCATC 3 cut(s) 49, 224, 719
MaeI CTAG 5 cut(s) 308, 455, 522, 656, 680
MaeII ACGT 3 cut(s) 105, 121, 260
MaeIII GTNAC 3 cut(s) 37, 353, 649
MalI GATC 4 cut(s) 118, 312, 342, 690
MboI GATC 4 cut(s) 116, 310, 340, 688
MboII GAAGA 3 cut(s) 106, 479, 732
MflI RGATCY 2 cut(s) 310, 688
MhlI GDGCHC 1 cut(s) 111
MluCI AATT 5 cut(s) 169, 389, 598, 636, 726
MlyI GAGTC 2 cut(s) 582, 646
MmeI TCCRAC 3 cut(s) 243, 550, 568
MnlI CCTC 7 cut(s) 6, 143, 421, 431, 463, 549, 748
MseI TTAA 5 cut(s) 233, 345, 363, 393, 757
MspCI CTTAAG 2 cut(s) 232, 344
MspR9I CCNGG 1 cut(s) 330
MvaI CCWGG 1 cut(s) 330
NdeII GATC 4 cut(s) 116, 310, 340, 688
NlaIII CATG 3 cut(s) 253, 445, 466
NmuCI GTSAC 1 cut(s) 649
NspI RCATGY 1 cut(s) 445
PciSI GCTCTTC 1 cut(s) 89
PfeI GAWTC 1 cut(s) 377
PkrI GCNGC 1 cut(s) 166
PleI GAGTC 2 cut(s) 581, 646
PmaCI CACGTG 1 cut(s) 122
PmlI CACGTG 1 cut(s) 122
PpsI GAGTC 2 cut(s) 581, 646
Ppu21I YACGTR 2 cut(s) 106, 122
Psp6I CCWGG 1 cut(s) 328
PspCI CACGTG 1 cut(s) 122
PspGI CCWGG 1 cut(s) 328
PspPI GGNCC 1 cut(s) 130
PsuI RGATCY 2 cut(s) 310, 688
RsaI GTAC 2 cut(s) 104, 193
RsaNI GTAC 2 cut(s) 103, 192
SapI GCTCTTC 1 cut(s) 89
SaqAI TTAA 5 cut(s) 233, 345, 363, 393, 757
SatI GCNGC 1 cut(s) 165
Sau3AI GATC 4 cut(s) 116, 310, 340, 688
Sau96I GGNCC 1 cut(s) 130
SchI GAGTC 2 cut(s) 582, 646
ScrFI CCNGG 1 cut(s) 330
SduI GDGCHC 1 cut(s) 111
SetI ASST 9 cut(s) 17, 108, 124, 193, 239, 263, 309, 518, 740
SfaNI GCATC 3 cut(s) 49, 224, 719
SfcI CTRYAG 1 cut(s) 480
SmlI CTYRAG 3 cut(s) 232, 344, 511
SmoI CTYRAG 3 cut(s) 232, 344, 511
Sse9I AATT 5 cut(s) 169, 389, 598, 636, 726
SspMI CTAG 5 cut(s) 308, 455, 522, 656, 680
StyD4I CCNGG 1 cut(s) 328
TaaI ACNGT 3 cut(s) 226, 398, 696
TaiI ACGT 3 cut(s) 108, 124, 263
TasI AATT 5 cut(s) 169, 389, 598, 636, 726
TfiI GAWTC 1 cut(s) 377
Tru1I TTAA 5 cut(s) 233, 345, 363, 393, 757
Tru9I TTAA 5 cut(s) 233, 345, 363, 393, 757
TscAI CASTG 4 cut(s) 142, 231, 343, 403
TseFI GTSAC 1 cut(s) 649
TseI GCWGC 1 cut(s) 164
Tsp45I GTSAC 1 cut(s) 649
TspDTI ATGAA 7 cut(s) 102, 260, 266, 390, 451, 495, 675
TspGWI ACGGA 2 cut(s) 199, 648
TspRI CASTG 4 cut(s) 142, 231, 343, 403
Vha464I CTTAAG 2 cut(s) 232, 344
XapI RAATTY 3 cut(s) 389, 598, 726
XbaI TCTAGA 1 cut(s) 655
XceI RCATGY 1 cut(s) 445
XcmI CCANNNNNNNNNTGG 1 cut(s) 542
XspI CTAG 5 cut(s) 308, 455, 522, 656, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.