Rh2CG369300

Lys-63-specific deubiquitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0021289
Physical Location & Seq
Reverse (-)
50178353 .. 50180420
2068 bp
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UTR
Exon/CDS
Intron
Rh2CG369300.1

Sequence Viewer

Length: 258 bp
ATGTCCTTAACGTGTGTGACAATGTCGGAGGACGTCTGGTTAACTTGTATGACTCATGCATTGTCCACCGAGACCGAGGAGATCATGGGCCTCCTTCTTGGTGATATTGAGAACTCTGCAAATGGGGGTGTGACTGCCGTGATTTGGGGAGCGTCACCTCAAACTCGATCAGACCGGAGGAAGGATCGTGTTGAAACTAATCCTGAGCAGTTGGCTGCTGCATCAGCTCAAGCTGAAATATCCTTGATGCTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0003674 GO:0003824 GO:0004843 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006282 GO:0006302 GO:0006325 GO:0006464 GO:0006508 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0008237 GO:0009314 GO:0009628 GO:0009893 GO:0009987 GO:0010212 GO:0010564 GO:0010604 GO:0010948 GO:0016043 GO:0016569 GO:0016570 GO:0016578 GO:0016579 GO:0016787 GO:0019219 GO:0019222 GO:0019538 GO:0019783 GO:0023052 GO:0031323 GO:0031325 GO:0031570 GO:0031572 GO:0031593 GO:0032991 GO:0033554 GO:0034641 GO:0035556 GO:0036211 GO:0036459 GO:0042770 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045739 GO:0045786 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051276 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0070011 GO:0070531 GO:0070536 GO:0070537 GO:0070552 GO:0070646 GO:0070647 GO:0071704 GO:0071840 GO:0072395 GO:0072401 GO:0072422 GO:0072425 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0101005 GO:0140030 GO:0140096 GO:1901360 GO:1901564 GO:1901987 GO:1901988 GO:1902749 GO:1902750 GO:2001020 GO:2001022
KEGG Pathways
Metabolic & Signaling
ko03440 ko04621 map03440 map04621
Pfam Domains
Protein Families
JAB

Protein Analysis

85

Amino Acids

9.17

Weight (kDa)

4.34

Isoelectric Point (pI)

62.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JAB PF01398 3 - 67 6.3e-06 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021289)

Species Orthologous Gene IDs
pyrus_communis pycom17g20820
rosa_chinensis RchiOBHm_Chr2g0136631
rosa_multiflora Rmu_sc0006440.1_g000006
rosa_samantha Rh2BG389600 Rh2CG369300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 36
AclWI GGATC 1 cut(s) 192
AcyI GRCGYC 1 cut(s) 33
AfiI CCNNNNNNNGG 2 cut(s) 144, 181
AflIII ACRYGT 1 cut(s) 11
AgsI TTSAA 1 cut(s) 194
AluBI AGCT 2 cut(s) 227, 233
AluI AGCT 2 cut(s) 227, 233
Alw26I GTCTC 1 cut(s) 65
AlwI GGATC 1 cut(s) 192
AoxI GGCC 1 cut(s) 88
ApeKI GCWGC 2 cut(s) 215, 218
AspS9I GGNCC 1 cut(s) 88
AsuHPI GGTGA 2 cut(s) 113, 147
BbvI GCAGC 2 cut(s) 202, 205
BceAI ACGGC 1 cut(s) 122
BcoDI GTCTC 1 cut(s) 65
BisI GCNGC 2 cut(s) 216, 219
BlsI GCNGC 2 cut(s) 217, 220
BmgT120I GGNCC 1 cut(s) 88
BmsI GCATC 2 cut(s) 230, 237
Bpu10I CCTNAGC 1 cut(s) 204
BpuEI CTTGAG 1 cut(s) 213
BsaHI GRCGYC 1 cut(s) 33
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 75
BsaWI WCCGGW 1 cut(s) 174
Bsc4I CCNNNNNNNGG 2 cut(s) 144, 181
BseDI CCNNGG 1 cut(s) 75
BseLI CCNNNNNNNGG 2 cut(s) 144, 181
BseMII CTCAG 1 cut(s) 195
BseRI GAGGAG 1 cut(s) 92
BseXI GCAGC 2 cut(s) 202, 205
BshFI GGCC 1 cut(s) 90
BsiSI CCGG 1 cut(s) 175
BslI CCNNNNNNNGG 2 cut(s) 144, 181
BsmAI GTCTC 1 cut(s) 65
BsnI GGCC 1 cut(s) 90
Bso31I GGTCTC 1 cut(s) 65
Bsp143I GATC 3 cut(s) 81, 167, 184
BspANI GGCC 1 cut(s) 90
BspCNI CTCAG 1 cut(s) 196
BspPI GGATC 1 cut(s) 192
BspTNI GGTCTC 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 75
BssMI GATC 3 cut(s) 81, 167, 184
BssNI GRCGYC 1 cut(s) 33
BstACI GRCGYC 1 cut(s) 33
BstDEI CTNAG 1 cut(s) 204
BstKTI GATC 3 cut(s) 84, 170, 187
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 3 cut(s) 81, 167, 184
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstV1I GCAGC 2 cut(s) 202, 205
BsuRI GGCC 1 cut(s) 90
Cfr13I GGNCC 1 cut(s) 88
CseI GACGC 1 cut(s) 141
CviAII CATG 2 cut(s) 56, 85
CviJI RGCY 4 cut(s) 90, 215, 227, 233
CviKI_1 RGCY 4 cut(s) 90, 215, 227, 233
DdeI CTNAG 1 cut(s) 204
DpnI GATC 3 cut(s) 83, 169, 186
DpnII GATC 3 cut(s) 81, 167, 184
Eco31I GGTCTC 1 cut(s) 65
EcoT22I ATGCAT 1 cut(s) 61
FaeI CATG 2 cut(s) 59, 88
FaiI YATR 3 cut(s) 50, 57, 86
FatI CATG 2 cut(s) 55, 84
Fnu4HI GCNGC 2 cut(s) 216, 219
Fsp4HI GCNGC 2 cut(s) 216, 219
GluI GCNGC 2 cut(s) 216, 219
HaeIII GGCC 1 cut(s) 90
HapII CCGG 1 cut(s) 175
HgaI GACGC 1 cut(s) 141
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 2 cut(s) 59, 88
HincII GTYRAC 1 cut(s) 42
HindII GTYRAC 1 cut(s) 42
HinfI GANTC 1 cut(s) 52
HpaI GTTAAC 1 cut(s) 42
HpaII CCGG 1 cut(s) 175
HphI GGTGA 2 cut(s) 113, 147
Hpy166II GTNNAC 2 cut(s) 42, 66
Hpy188I TCNGA 2 cut(s) 28, 172
Hpy188III TCNNGA 1 cut(s) 203
Hpy8I GTNNAC 2 cut(s) 42, 66
HpyAV CCTTC 2 cut(s) 104, 175
HpyCH4IV ACGT 2 cut(s) 11, 33
HpyCH4V TGCA 3 cut(s) 59, 119, 221
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpyF3I CTNAG 1 cut(s) 204
HpySE526I ACGT 2 cut(s) 11, 33
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 2 cut(s) 59, 88
KspAI GTTAAC 1 cut(s) 42
Kzo9I GATC 3 cut(s) 81, 167, 184
LmnI GCTCC 1 cut(s) 149
LpnPI CCDG 3 cut(s) 22, 188, 216
Lsp1109I GCAGC 2 cut(s) 202, 205
LweI GCATC 2 cut(s) 230, 237
MaeII ACGT 2 cut(s) 11, 33
MaeIII GTNAC 3 cut(s) 16, 130, 153
MalI GATC 3 cut(s) 83, 169, 186
MboI GATC 3 cut(s) 81, 167, 184
MlyI GAGTC 1 cut(s) 46
MmeI TCCRAC 1 cut(s) 6
MnlI CCTC 5 cut(s) 22, 70, 101, 168, 171
Mph1103I ATGCAT 1 cut(s) 61
MseI TTAA 3 cut(s) 8, 41, 256
MspI CCGG 1 cut(s) 175
MwoI GCNNNNNNNGC 1 cut(s) 224
NdeII GATC 3 cut(s) 81, 167, 184
NlaIII CATG 2 cut(s) 59, 88
NmuCI GTSAC 3 cut(s) 16, 130, 153
NsiI ATGCAT 1 cut(s) 61
PflFI GACNNNGTC 1 cut(s) 22
PkrI GCNGC 2 cut(s) 217, 220
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
PspPI GGNCC 1 cut(s) 88
PsyI GACNNNGTC 1 cut(s) 22
SaqAI TTAA 3 cut(s) 8, 41, 256
SatI GCNGC 2 cut(s) 216, 219
Sau3AI GATC 3 cut(s) 81, 167, 184
Sau96I GGNCC 1 cut(s) 88
SchI GAGTC 1 cut(s) 46
SetI ASST 5 cut(s) 14, 36, 160, 229, 235
SfaNI GCATC 2 cut(s) 230, 237
SmlI CTYRAG 1 cut(s) 228
SmoI CTYRAG 1 cut(s) 228
TaiI ACGT 2 cut(s) 14, 36
TaqI TCGA 1 cut(s) 166
TaqII GACCGA 1 cut(s) 89
Tru1I TTAA 3 cut(s) 8, 41, 256
Tru9I TTAA 3 cut(s) 8, 41, 256
TseFI GTSAC 3 cut(s) 16, 130, 153
TseI GCWGC 2 cut(s) 215, 218
Tsp45I GTSAC 3 cut(s) 16, 130, 153
Tth111I GACNNNGTC 1 cut(s) 22
ZraI GACGTC 1 cut(s) 34
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.