Rh2CG650300

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0023100
Physical Location & Seq
Forward (+)
81837135 .. 81839316
2182 bp
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UTR
Exon/CDS
Intron
Rh2CG650300.1

Sequence Viewer

Length: 225 bp
ATGATGGCGGCTTCTGCGGCGGTGACTTACAAGAATGGGAAATCGGAGATTCACTCTGTCCCCGATTCGCCGATGCTCGAGAACTCGTCGTCGTTTGGGTTAACATCGTCATCTCCGTCACTGGCCAGTTTGCCTCCGATTCGGATCCACAGTGGCAATAGAACGCAGGTGAGTCCTATTTGCAGTCTCGTGGAAACAGCAAGCGAGGGAGCAGAGAAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

74

Amino Acids

7.7

Weight (kDa)

6.02

Isoelectric Point (pI)

52.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0023100)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003835.1_g000013
rosa_samantha Rh2AG365000 Rh2CG650300 Rh2DG701200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 157
Acc36I ACCTGC 1 cut(s) 157
AciI CCGC 3 cut(s) 8, 17, 20
AclWI GGATC 2 cut(s) 139, 152
AcoI YGGCCR 1 cut(s) 123
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 2 cut(s) 139, 152
Ama87I CYCGRG 1 cut(s) 77
AoxI GGCC 1 cut(s) 123
AsuHPI GGTGA 2 cut(s) 34, 181
AvaI CYCGRG 1 cut(s) 77
BalI TGGCCA 1 cut(s) 125
BamHI GGATCC 1 cut(s) 144
BauI CACGAG 1 cut(s) 188
BcoDI GTCTC 1 cut(s) 191
BfaI CTAG 1 cut(s) 223
BfuAI ACCTGC 1 cut(s) 157
BisI GCNGC 2 cut(s) 9, 18
BlsI GCNGC 2 cut(s) 10, 19
BmeT110I CYCGRG 1 cut(s) 77
BmiI GGNNCC 1 cut(s) 146
BmsI GCATC 1 cut(s) 63
BplI GAGNNNNNCTC 2 cut(s) 38, 70
BsaBI GATNNNNATC 1 cut(s) 143
Bse1I ACTGG 2 cut(s) 126, 126
Bse8I GATNNNNATC 1 cut(s) 143
BseJI GATNNNNATC 1 cut(s) 143
BseNI ACTGG 2 cut(s) 126, 126
BshFI GGCC 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 77
BslFI GGGAC 1 cut(s) 44
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 44
BsnI GGCC 1 cut(s) 125
BsoBI CYCGRG 1 cut(s) 77
Bsp143I GATC 1 cut(s) 144
BspACI CCGC 3 cut(s) 8, 17, 20
BspANI GGCC 1 cut(s) 125
BspLI GGNNCC 1 cut(s) 146
BspMI ACCTGC 1 cut(s) 157
BspPI GGATC 2 cut(s) 139, 152
BsrI ACTGG 2 cut(s) 126, 126
BssMI GATC 1 cut(s) 144
BssSI CACGAG 1 cut(s) 188
Bst2BI CACGAG 1 cut(s) 188
Bst4CI ACNGT 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 202
BstKTI GATC 1 cut(s) 147
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 1 cut(s) 144
BstMWI GCNNNNNNNGC 2 cut(s) 14, 17
BstX2I RGATCY 1 cut(s) 144
BstYI RGATCY 1 cut(s) 144
BsuRI GGCC 1 cut(s) 125
BtsIMutI CAGTG 2 cut(s) 119, 157
BveI ACCTGC 1 cut(s) 157
Cac8I GCNNGC 1 cut(s) 202
CviJI RGCY 2 cut(s) 11, 125
CviKI_1 RGCY 2 cut(s) 11, 125
DpnI GATC 1 cut(s) 146
DpnII GATC 1 cut(s) 144
EaeI YGGCCR 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 77
FaqI GGGAC 1 cut(s) 44
Fnu4HI GCNGC 2 cut(s) 9, 18
Fsp4HI GCNGC 2 cut(s) 9, 18
FspBI CTAG 1 cut(s) 223
GluI GCNGC 2 cut(s) 9, 18
HaeIII GGCC 1 cut(s) 125
HincII GTYRAC 1 cut(s) 102
HindII GTYRAC 1 cut(s) 102
HinfI GANTC 4 cut(s) 49, 65, 139, 172
HpaI GTTAAC 1 cut(s) 102
HphI GGTGA 2 cut(s) 34, 181
Hpy166II GTNNAC 1 cut(s) 102
Hpy188I TCNGA 3 cut(s) 46, 138, 144
Hpy188III TCNNGA 1 cut(s) 79
Hpy8I GTNNAC 1 cut(s) 102
Hpy99I CGWCG 2 cut(s) 91, 94
HpyCH4III ACNGT 1 cut(s) 152
HpyCH4V TGCA 1 cut(s) 183
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 17
KspAI GTTAAC 1 cut(s) 102
Kzo9I GATC 1 cut(s) 144
LmnI GCTCC 1 cut(s) 209
LpnPI CCDG 3 cut(s) 107, 139, 152
LweI GCATC 1 cut(s) 63
MaeI CTAG 1 cut(s) 223
MaeIII GTNAC 2 cut(s) 22, 117
MalI GATC 1 cut(s) 146
MboI GATC 1 cut(s) 144
MflI RGATCY 1 cut(s) 144
MlsI TGGCCA 1 cut(s) 125
MluNI TGGCCA 1 cut(s) 125
MlyI GAGTC 1 cut(s) 181
MnlI CCTC 2 cut(s) 144, 199
Mox20I TGGCCA 1 cut(s) 125
MscI TGGCCA 1 cut(s) 125
MseI TTAA 1 cut(s) 101
Msp20I TGGCCA 1 cut(s) 125
MwoI GCNNNNNNNGC 2 cut(s) 14, 17
NdeII GATC 1 cut(s) 144
NlaIV GGNNCC 1 cut(s) 146
NmuCI GTSAC 2 cut(s) 22, 117
PaeR7I CTCGAG 1 cut(s) 77
PaqCI CACCTGC 1 cut(s) 157
PcsI WCGNNNNNNNCGW 1 cut(s) 113
PfeI GAWTC 3 cut(s) 49, 65, 139
PkrI GCNGC 2 cut(s) 10, 19
PleI GAGTC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 180
PspN4I GGNNCC 1 cut(s) 146
PsuI RGATCY 1 cut(s) 144
SaqAI TTAA 1 cut(s) 101
SatI GCNGC 2 cut(s) 9, 18
Sau3AI GATC 1 cut(s) 144
SchI GAGTC 1 cut(s) 181
SetI ASST 1 cut(s) 171
SfaNI GCATC 1 cut(s) 63
Sfr274I CTCGAG 1 cut(s) 77
SlaI CTCGAG 1 cut(s) 77
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
SsiI CCGC 3 cut(s) 8, 17, 20
SspMI CTAG 1 cut(s) 223
TaaI ACNGT 1 cut(s) 152
TaqI TCGA 1 cut(s) 78
TauI GCSGC 2 cut(s) 11, 20
TfiI GAWTC 3 cut(s) 49, 65, 139
Tru1I TTAA 1 cut(s) 101
Tru9I TTAA 1 cut(s) 101
TscAI CASTG 2 cut(s) 126, 157
TseFI GTSAC 2 cut(s) 22, 117
Tsp45I GTSAC 2 cut(s) 22, 117
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 2 cut(s) 126, 157
XhoI CTCGAG 1 cut(s) 77
XspI CTAG 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.